Title of Invention	A PROCESS OF PRODUCING A BIOTIN VITAMER
Abstract	Abstract A process is disclosed for the increased production of biotin and the biotin precursor dethiobiotin using a bacterium that produces a lysine-utilizing DAPA aminotransferase. The process involves the use of a bacterium that is either grown in the presence of lysine or deregulated for lysine biosynthesis.

Full Text

Accordingly the present invention provides a process of producing a biotin vitamer by: (c) culturing a bacterium comprising a lysine-utilizing DAPA aminotransferase whereby either said culturing takes place in an environment enriched for lysine, a lysine analog, or a lysine precursor or whereby said bacterium is deregulated with respect to lysine production and in each case (d) recovering said biotin vitamer. In the following a brief description of the accompanying drawings and tables is given: Accordingly the present invention provides a process of producing a biotin vitamer by: (c)culturing a bacterium comprising a lysine-utilizing DAPA aminotransferase whereby either said culturing takes place in an environment enriched for lysine, a lysine analog, or a lysine precursor or whereby said bacterium is deregulated with respect to lysine production and in each case (d) recovering said biotin vitamer. In the following a brief description of the accompanying drawings and tables is given: WE CLAIM: 1. A process of producing a biotin vitamer by: (a)culturing a bacterium comprising a lysine-utilizing DAPA aminotransferase whereby either said culturing takes place in an environment enriched for lysine, a lysine analog, or a lysine precursor or whereby said bacterium is deregulated with respect to lysine production and in each case (b) recovering said biotin vitamer. 2. The process as claimed in claim 1, in which the bacterium is engineered to overproduce a lysine-utilizing DAPA aminotransferase. 3. The process as claimed in claim 1 or 2, in which lysine, a lysine analog, or a lysine precursor is exogeneously added to the culture. 4. The process of any one of claims 1 to 3, in which the biotin vitamer is biotin, 7,8-diaminopelargonic acid (DAPA) or dethiobiotin and more specifically after recovering the dethiobiotin, the method further comprises converting the recovered dethiobiotin to biotin by a separate fermentation, biochemical reaction, or chemical reaction and recovering biotin. 5. The process as claimed in any one of claims 1 to 4, in which the bacterium is resistant to a lysine analog, e.g. S-2-aminoethyl-L-cysteine (AEC). 6. The process as claimed in claim 1, in which the bacterium is deregulated with respect to at least one biotin synthetic pathway step in addition to bioA expression. 7. The process as claimed in any one of claims 1 to 5, in which the bacterium is further engineered to produce a SAM-utilizing DAPA aminotransferase. 8. The process as claimed in claim 7, in which methionine, S- adenosylmethionine (SAM), or an analog of SAM is added to the culture. 9. A process of producing a biotin vitamer substantially as herein above described and illustrated with reference to the accompanying drawings.

Documents:

1547-mas-98 claims granted.pdf

1547-mas-98 description(complete) gratned.pdf

1547-mas-98 drawings granted.pdf

1547-ms-1998 abstract.pdf

1547-ms-1998 claims.pdf

1547-ms-1998 correspondnece-others.pdf

1547-ms-1998 correspondnece-po.pdf

1547-ms-1998 description(complete).pdf

1547-ms-1998 form-2.pdf

1547-ms-1998 form-26.pdf

1547-ms-1998 form-3.pdf

1547-ms-1998 form-4.pdf

1547-ms-1998 form-6.pdf

1547-ms-1998 petition.pdf