Title of Invention

METHOD OF PRODUCING A HEALTHY FOOD

Abstract Method of producing a healthy food involves obtaining metabolite by culturing mixed cells of a purple photosynthetic bacterium (for example, Rhodopseudomonas capsulate FERMBP- 7434 strain) with a lactic acid bacterium (for example, lactobacillus). These rhealth foods contribute to the maintenance of human health in good conditions or regain thereof even at a small dose causing little burden.
Full Text The present invention has an object to provide a strain of the red photosynthetic bacterium and the health food prepared with same, which are effective for maintaining and recovering the health,
DISCLOSURE OF INVENTION
The inventors of the present invention, as a result of intensive study on the red photosynthetic bacterium which was incubated in various methods, in order to attain the foregoing objects, found out that the red photosynthetic bacterium, which is incubated under a specific condition, is effective for maintaining or recovering health, to accomplish the present invention.
In short, the red photosynthetic bacterium of the present invention is characterized by being Rhodopseudomonas capsulate FERMBP-7434 strain, in order to attain the object.
With the above arrangement, use of FERMBP-7434 strain achieves stable production of a healthy food that is excellently effective for maintaining or recovering the health.
The healthy food of the present invention, in order to attain the foregoing objects, is characterized by including a metabolic product obtained by incubation of the photosynthetic bacterium so as to make the photosynthetic bacterium produce a viscous material.
With the above arrangement, ingestion of the metabolic product, which is obtained by incubation of the photosynthetic bacterium so as to make the photosynthetic bacterium produce a viscous material, can recover or maintain health of a person who ingests the metabolic product.

specific incubation substances (NH4)2S04, KH2p04, MgS04 7H20, NaCI, NaHCOg, and yeast extract (including the above mentioned growth factors) . In case of the incubation of the non-sulfur bacterium, low fatty acids such as acetic acid, propionic acid, and lactic acid, which were in a form of Na salt, were added into the base medium, so as to prepare the liquid medium (for example, at pH 7,0). Moreover, in case of the incubation of the red sulfur bacterium, NajS-QHaO was added into the base medium and adjusted by using KOH solution so as to prepare a liquid medium (at between pH 8.2 and 8.5)-
Next, the liquid medium was transferred from the mixing tank for nutrition to a sealed and illuminated growth tank. Then, as the photosynthetic bacterium, for example, Rhodopseudomonas capsulatas FERMBP-7434 strain, which was a red non-sulfur bacterium (Athiorhodaceae), was inoculated into the sealed and
illuminated growth tank.
In this case, human waste or waste liquid of shochu (Japanese distilled white liquor), in which organic acids had been generated, might be poured directly into the sealed and illuminated growth tank, instead of the liquid medium. Note that, the photosynthetic bacterium of this type also metabolizes starch, glucose, sucrose, alcohol, and other high molecular carbohydrates, thereby growing well, if various heterotrophic bacteria coexist, besides the organic acids that form the liquid medium. Because of this, it is more effective to inoculate, in the sealed arvd illuminated growth tank, various heterotrophic bacteria, such as the above-mentioned lactic acid bacteria, together with the photosynthetic bacterium, while adding those high molecular carbohydrates into the liquid medium. In addition, hydrogen gas generated during the incubation of the photosynthetic bacterium can be stored in a tank so as to be used as a fuel.
Consequently, the bacterial solution, which had been incubated to an optimum level in the sealed and illuminated growth tank, was converted into the

concentrated liiomass by gathering the bacteria by means of a continuous centrifugal separator. Thereafter, the concentrated biomass was freeze-dried so as to obtain a dehydrated biomass. In the above process, when the incubated bacterial soiution is transferred into the continuous centrifugal separator, it is possible to continuously obtain the identical photosynthetic bacterium, when, for example. 20% of the whole solution is always left in the growth tanit so that the liquid medium prepared in the mixing tank for nutrition is added to the 20% of the liquid medium.
Note that, the reason why the sealed and illuminated growth tank was used in this method was because the photosynthetic bacterium grows optimally in the anaerobic atmosphere and under the illuminated condition (between 3000 lux and 10000 lux). Moreover, a stirring apparatus for stirring the liquid medium may be provided in the sealed and illuminated growth tank. The provision of the stirring apparatus can improve grovirth speed of the bacteria.
[EXAMPLE]
Described below is an example of the incubation of the photosynthetic bacterium. To begin with, in the mixing tank for nutrition, added Into water of 1 x cm^ (1 litre) were:
(NH4) 2SO4 0.3g
KHJPOA 0.5g
MgS047H20 0.2g
NaCI 0.5g
NaHCOs 0.2g
Yeast Extract 0.01g
The respective above-listed nutrition were mixed into the water to prepare the

base medium. Further, acetic acid 0.4 weight %, in the form of Na salt, and sucrose 5 weight % were added into the base medium, whicti was further adjusted to pH 7.0, for example, to prepare the liquid medium. Then, the liquid medium was transferred into the sealed and illuminated growth tank.
The growth tank, made of a transparent material, such as glass, in a cylinder-like shape, was illuminated by fluorescent lamps arranged in periphery of the growth tank at regular interval so as to illuminate evenly an inside of the growth tank. IVleanwhile, the growth tank was provided with a stirring device that has blades of a size of a radius of the growrth tank, in the growth tank. Therefore, the growrth tank was capable of incubating the photosynthetic bacterium in a large quantity and with ease in the anaerobic atmosphere.
Next, a solution (bacterial concentration 10^ cell/cm^) of Rhodopseudomonus capsulatas FERMBP-7434 strain was inoculated in a 20% ratio (v/v) over the total of the solution in the growth tank, then, a small quantity of a lactic acid bacterium (Lactobacillus bulgalicus, bacterial concentration 10^ cell/cm^) was inoculated. The liquid medium was stirred at a rotation speed 13 times per minute at an incubation temperature 30 °C under illumination of 10000 lux. Alter 8 hours, the growth of the photosynthetic bacterium attained its optimum (stationary phase). Here, in the growth tank, a large quantity of the viscous material had been produced, while the photosynthetic bacterium was grown.
This liquid medium was transferred into a continuous centrifugal separator (sharp less type) so as to gather and concentrate the bacteria. The concentrated btomass was subjected to the freeze-drying. so that the biomass was obtained. The Thus obtained biomass could be inoculated and obtained in a ratio of about 5 g per 1 X 10^ (1 litre) of the liquid medium. As discussed later, the obtained biomass was quite active.

In the following, explained is a process of the freeze-drying. To begin with, the thus obtained concentrated biomass (about 10" cell/cm^) was freezed to store in a freezer, once. At the time of the freeze-drying, for example, 4x10^ cm3 (4 litre) was naturally thawed (about 12 hours), then poured and divided into 9 sucking bottles for 1.2 x 10^ cm^ approximately evenly (about 440 cm^ each bottle).
Consequently, in a preliminary freezing tank (- 45 °C), which had been filled in advance with an anti-freezing solution such as methanol, a bottom of the sucking bottles was touched with the antl-freezing solution by means of a prefreezer, while the sucking tiottles were rotated, so that the concentrated tiiomass in the sucking bottle was freezed again so as to form a thin film along an inner wall of the sucking bottle (it was arranged that a thickness of the freezed biomass in the sucking bottle was about 8 mm, and the freezing time was about 20 minutes) . The freezed concentrated biomass was stored in the freezer until the freezing of all the 9 bottles were completed.
After that, an inside of a trap of a freeze-drler was cooled down (-45 °C). After one hour since then (that is. when the cooling in the trap was completed), a vacuum pump was operated. After it was confirmed that a vacuum gauge of the vacuum pump was lowered below 26 Pa, preferably 4 to 6 Pa, the respective sucking bottles were connected with the trap. Consequently, the respective sucking bottles and the vacuum pump were linked via the trap. Then, the drying of the freezed biomass inside the respective sucking bottles was started at a room temperature (20 °C to 30 °C). The drying time, while the drying time depends on the room temperature, was about 40 hours. Note that, even though the above example used the freeze-drying method as the drying method of the concentrated biomass, it was also possible to use spray drying as another drying method.
The thus obtained dried biomass was milled, for example, by using a crushing

apparatus of a propeller-type {sample mill), where the rotation of the propellers was about 15000 rpm, so as to powder the dried biomass. Other powdering methods were, for example, a jet mill method or a ball mill method.
The powdered dried biomass may be used as the healthy food (tFK-RC) as it stands. Alternatively, the powdered dried biomass may be processed into a form of a tablet for the sake of easy ingestion. For example, a tablet-making machine of a high-speed rotation type may be used for making the tablet. At the time of the tablet making process, it is possible to make the tablet without using an excipient. such as lactose, a binder, and a releasing agent such as magnesium stearate. Note that, if necessary, it may be possible to use an excipient for adjusting a dose.
In the above, explained was the example where Rhodopseudomonas capsulatas FERI\flBP-7434 strain was used. However, it may be possible to use other photosynthetic bacteria, such as Chromatium vinosum in a Thiorhodaceae family, or Rhodospirillum Rubrum in an Athiorhodaceae family.
Each quantification method for bacteriochlorophyll and carotenoid materials in the healthy food (TKF-RC) were carried out, based on "photosynthetic researching method" (by Sakae Kato, Kyoritsu Publishing Company, 1981).
Described below is the qualification method for the bacteriochlorophyll. To begin with, about 100 mg of a sample of the dried healthy food (TFK-RC) was taken and measured, and suspended in a physiological saline solution, 100 mm^ (^tl). Further, 4.9 cm^ of acetone:methanol [7:2 (v/v)] was added. Then, the bacteriochlorophyll was extracted. Then, the extract was appropriately diluted. Absorbancy of the diluted solution at 770 nm was measured. A concentration of the bacteriochlorophyll was calculated out by the following equation (1):

Bacteriochlorophyll (ng/cm ) = 12,15 A77Q {1)
In the following, 5 lots of samples 1 through 5 of the bacteriochlorophyll of the present healthy food (TFK-RC), which was manufactured by the above method, were quantified, respectively. A result of the quantification is presented in Table 1. TABLE 1

SAMPLE NO SAMPLE (mg) WEIGHT A7T0 CONTENT (mg/g)
1 11,3 2,52 13,5
2 10,4 2,05 12,0
3 9.9 2,10 12,9
4 10,6 2.58 14.8
5 10.3 1.48 8,7
Note that, the absorbancy (770nm) indicated by A770
in Table 1 is a conversion value to an extracted stock solution (5 cm"), The result showed that the contents (weight %) of the bacteriochlorophyll in the healthy food (TFK-RC) were between 0.2 and 3.0, preferably, between 0.6 and 1.9.
In addition, because the measurement of the absorbancy was carried out at 770 nm (red region) in the quantification of the bacteriochlorophyll, it was noted that the measurement result of the bacteriochlorophyll was not affected at all, even if the carotenoid materials were contained in the diluted solution.
Next, the quantification method of the carotenoid materials is explained. To begin with, about 10 mg of a sample of the dried healthy food was taken and measured, and was suspended in methanol, boiled to extract for one minute, and

cooled down by ice, A supernatant was recovered by means of centrifugal separation, A precipitate was again suspended in the methanol. The extraction was repeated until a colorless extract was obtained, for example, for three limes.
Ether in an equal quantity, and water in a double quantity with respect to the methanol extract were added into the methanol extract, and ether extraction was carried out. Then, an ether solution, which was separated out, was dehydrated. The thus obtained ether solution was measured to make 6 cm^ of the ether solution. Then, absorption spectrum of the ether solution was measured.
An absorption maximum wavelength within a range from 400 to 550 nm of the absorption spectrum was determined, and absorbancy at the absorption maximum wavelength was measured. Using the absorbancy, the contents of the carotenoid materials were calculated out by the following equation (2):
C = Dv/r4x10^ (2)
c: content of carotenoid material (mol) D: absorbency at the absorption maximum wavelength, v: volume of ether solution (10^ cm^, that is, one litre),
1.4x10^ : an average molecular absorption coefficient of carotenoid material.
Because the maximum absorption of the carotenoid materials exists within the range from 400 to 550 nm, the absorption maximum wavelength within the range was measured from the absorption spectrum (see Figure 1) of the ether solution. The carotenoid materials were quantified, based on the absorbancy at the absorption maximum wavelength.

Shown below in Table 2, are each quantification result of the carotenoid material as to the respective samples 1 to 5 discussed above. Moreover, absorption spectrum of the sample 1 is illustrated in Figure 1. Absorption spectrums of the other samples 2 to 5 also showed a same pattern.
The result in Table 2 explains that contents {^mol/g) of the carotenoid materials in the healthy food (TFK-RC) were between 0.5 and 7.5, preferably between 2.4
and 4.0.
TABLE 2

SAMPLE NO SAMPLE WEIGHT (mg) A77Q CONTENT (mg/g)
1 9,4 0.756 3.45
2 10.8 0.868 3.44
3 11.4 0.782 2,94
4 9.6 0.666 2,97
5 10-4 0.733 3.02
ABBREVATION: A.M.W stands for absorbancy maximum wavelength.
Moreover, according to the result in Table 1, since no absorbancy was measured above 600nm in a visible region, it was found out that, at most, less than a quantity of a detection limit of the bacteriochlorophyll was contained in the ether extract. Therefore, as to the quantification method of the carotenoid materials, it was found out that the quantification of the carotenoid materials was not affected at all, even though the bacteriochlorophyll was contained in the healthy food (TFK-RC).

Next, as to the respective samples 1 to 5 of the healthy food, and water-washed samples of the samples 1 to 5, were respectively subjected to acid hydrolysis, and then quantified in terms of the following respective neutral monosaccharides, by means of a high performance liquid chromatographic method.
The quantification method is described below. To begin with, as to preparation of the water-washed samples, about 0.5g of each sample was weighed and placed in a centrifugal tube. 25 cm^ of water was added into the centrifugal tube and stirred, then, was subjected to ultrasonic extraction for 3 minutes, then was subjected to centrifugal separation (12,000 rpm, 5 minutes) so as to remove a supernatant. 25 cm^ of water was added into a reside in the centrifugal tube and the water-washing process was processed again for two times in the same manner.
The reside, to which 25 cm^ of Acetone was added in order to remove water, was stirred, then was subjected to centrifugal separation (12,000 rpm, 5 minutes) so as to remove a supernatant- After acetone remained in the centrifugal tube was volatilized under a nitrogen stream, the reside was air-dried to be the water-washed sample.
Next, explained is preparation of a test solution. To begin with, after 0.3 g to 0.6 g of each sample or 0.3 g to 0.6 g of each water-washed sample were weighed, 4 cm^ of 72 % sulfuric acid was added to the samples and the water-washed samples. Then, the samples were stirred for one hour at a room temperature (the water-washed samples were stirred for two hours)
Consequently, the samples and the water-washed samples were diluted with 112 cm^ of water (sulfuric acid concentration: 4%), and were subjected to hydrolysis for one hour in an autoclave (121 °C). After the samples and the water-washed samples were cooled down to the room temperature, and neutralized by a

sodium hydroxide solution of 30 w/v%, their volumes were adjusted to 200 cm^ with water. Then, the samples and water-washed samples were filtered (No. 5B, supplied from Advantech Toyo Co,, Ltd.), and further filtered with a membrane fitter having a pore diameter of 0,45 jim, thereby obtaining a filtrate as the test solution.
The contents of monosaccharides {glucose, ribose, rhamnose, and fucose) were measured by the liquid chromatographic method. A result of the measurement is shown in Table 3. The measurement result indicates the contents (g) per 100 g of the healthy food (TFK-RC)
TABLE 3

1 2 3 4 5
GLUCOSE BEFORE WASHING 1 AFTER WASHING 5.1 2.1 4Z 2.2 4.S 2,2 5.0^ 2.2 5,1 1,7
RIBOSE BEFORE WASHING AFTER WASHING 0.7
0.5 0.7 0.6 0,7 0,5 0.7 0.6 0,7 0,6
RHAMNOSE BEFORE WASHING AFTER WASHING 2,0 0.9 2.0 0.8 2.0 0.8 2,0
0,9 2,2
1,0
FUCOSE BEFORE WASHING AFTER WASHING 1,2 0.2 1.2 ND 1.2 ND 1,2 ND 1.7 0.3

BEFORE WASHINC

In Table 3, ND indicates that the content was less than the detection limit (0,2g/100g),
According to the result in Table 3, it was found out that, in the acid hydrolyzed samples of the healthy food (TFK-RC) before washing, the contents (weight %) of glucose were in a range between 2,4 and 7.5, more preferably between 3.5 and 6,5, the contents (weight %) of ribose were in a range between 0,3 and 1,1,

more preferably between 0.4 and 1.0, the contents (weight %) of rhamnose were in a range between 1,0 and 3.3, more preferably between 1.2 and 3.0, the contents (weight %) of fucose were in a range between 0.6 and 2.6, more preferably 0.8 and 2,4,
Moreover, according to the result in Table 3, it was found out that, in the acid hydrolyzed sample of the healthy food (TFK-RC) after washing, the contents (weight %) of glucose were in a range between 0.8 and 3.3, more preferably between 1.0 and 3.0, the contents {weight %) of ribose were in a range between 0.2 and 1.0, more preferably between 0.3 and 0,9, the contents (weight %) of rhamnose were in a range between 0.4 and 2.0, more preferably between 0,5 and 1,6, the contents (weight %-) of fucose were less than 0.6, more preferably less than 0,5,
Next, as to the healthy food {TFK-RC) of the present invention, an acute oral toxicity test (limit test) was carried out. In short, the acute oral toxicity test (limit test) as to samples of the healthy food (TFK-RC) was carried out by using mice, in accordance with OECD (Organization for Economic Cooperation and Development) chemical substance test guide (1987).
A test group of male and female mice was subjected to single-time oral administration of 2,000mg/i The test is explained below. To begin with, the sample of the healthy food (TFK-RC) was suspended in purified water to prepare 100 mg/cm^of a test solution.

The tested animal was as follows. To being with, ICR-type male and female mice of 4 week old were purchased from Japan SLC Co., Ltd. After the mice were preliminarily kept for about one week for checking their general condition was not abnormal, the mice were used for the test. The tested animals were put in cages made of polycarbonate, which respectively contained 5 of the tested animals, and were kept in a breeding room in which a room temperature was set at 23 i 2 °C and illumination time was set at 12 hours per day. Feed (solid feed for mice and rats; lab MR stock, made by Japan agricultural products industry Co., Ltd.) and drinking water (tap water) were freely given.
The testing method was as follows. To begin with, both the tested group and the control group had 10 of the male and the female mice, respectively. Before the administration, the tested animals were fasted for about 4 hours. After their body weight was measured, the tested group, both the males and the females, was subjected to a forcible single-time oral administration of the test solution whose dosage, a sample administration amount, was 2,000 mg/kg, by using a stomach sonde. As to the control group, 0.6 cm^ of the purified water was administered to the males, and 0.5 cm^ of the purified water was administered to the females, in the same manner.
The observation period was 14 days. Observation was carried out frequently on the day of the administration. The obsen/ation was carried out once a day from the following day. On 7 days and 14 days since the administration, the body weight was measured, and a comparison between the groups was carried out by inspection with a 5% level of significance. At an end of the observation period, all the tested animals were anatomized. A result of the test was as shown in Table 4. In parentheses in Table 4, shown is a number of the animals.

TABLE 4

BEFORE AD AFTER AD (DAY)
ADMINISTRATED GROUPS

7 14
MALE TESTED G 28.2 + 0.8 33.9 + 1.3 37.7 + 2.0
£10) 110) £10)

CONTROL G 28.1 ±0.8 33,8 +0.8 36.8 + 1.8
£10) £10) £10)
FEMALE TESTED G 24.3 ± 0.6 27.0+ .2 28,9 + 1.4
£10) £10) £10)

CONTROL G 24.0 + 0.5 27,4+1.6 29.3+ 1.9
110) £10) £10)
ABBF DEVIATION: AD. STANDS . FORADMINISl rRATION.
G. STANDS FOR GROUP.
In the above test, no expiry was observed for both the males and the females during the observation period. No abnormality was observed for both the males and the females during the observation period. As to the body weight measurement on 7 days and 14 days since the administration, no difference between the groups in terms of weight gain was observed as for both the males and the females, as shown in Table 4. In the anatomy after the observation period, no abnormality was found in main internal organs of all the tested animals for both the males and the females.

According to the OECD chemical substance test guide (1987), it is instructed that an intensive test for determining an LDjo value is necessary in case expiry is observed with dosage of 2000 mg/kg.
However, in the above test result, no expiry was observed with this dosage, and no abnormality was found at the anatomy, too. Therefore, it was judged that the LDso value of the single-time oral administration to the tested mice was more than or equal to 2000mg/kg for both the males and the females.
Because of this, it was proved that the healthy food (TKF-RC) of the present
invention does not adversely affect a human body even in case of regular intake of the healthy food (TKF-RC).
In the following, morphological characteristics, growth conditions, and physiological characteristics of Rhodopseudomonas capsulatas are described.
a. Morphological Characteristics
Rhodopseudomonas capsulatas has a flagellum and is quite motile. Generally, they are short bacilli (width 0.5 ji. x length 1.0 ^.), while some are long bacilli (width 0.5 fi. to 0.7 n, x length 6.0 ji.), depending on a type of liquid media and incubation periods. In other words, they show polymorphism.
b. Growth Conditions
The growth result (anaerobic and under illumination) on various media
are described below.
Meat Extract + Lactic Acid ++
Peptone Water +++ Succinic Acid +
Potato Medium - Malic Acid +

Thiosulfate - Butyric Acid ++
Alanine + Crotonic Acid +
Leucine - Pyruvic Acid ++
Asparagine + Ethanol +
Aspartic Acid - Mannitol
Glutamic Acid + Sorbitol
Tartaric Acid - Mannose
Citric Acid - Fructose
Glutaric Acid + Glycerol
Acetic Acid +
Propionic Acid +++
(All the substrates were used in 0.2 weight % concentration.) Note: +++ Growth was good.
+ Growth was possible.
Growth was impossible.
c. Physiolcgical Characteristics 1) Optimal Growth Condition
pH 7.2. temperature 27 °C.
anaerobic illumination 10,000 lux
2) Condition which allows the growth
pH 6.0 to pH 8.5, temperature 23 °C to 39°C. aerobic to anaerobic dark condition to illumination condition

3) Gram Staining Characteristics
Negative
4) Anti-acid Characteristics Positive
5) Indole production Negative
6) Hydrogen Sulfide Production Negative
7) Ability for Nitrogen Gas Fixation Positive
8) It also carries out denitrification in a nitrate medium, in which nitric acid is
reduced and converted to a gas of N2, on contrary to the nitrogen fixafion.
9) Catalase Production Positive
10) Gelatine Liquefaction Negative
11) Starch Hydrolysis Negative
12) Ability to oxidize Methylene Blue of a reduction type. Methyl (or Benzyl)
Biorodien pigment of a reduction type
Positive

13) It requires Biotin, Thiamin, and Nicotinic Acid as growth factors.
4
The invention being thus described, it will be obvious that the same way may be varied in many ways. Such variations are not to be regarded as a departure from the spirit and scope of the invention, and all such modifications as would be obvious to one skilled in the art are intended to be included within the scope of the claims made.
INDUSTRIAL APPLICABILITY
A red photosynthetic bacterium of the present invention is, as discussed above, Rhodopseudomonas capsulatas FERMBP-7434 strain.
Therefore, because the red photosynthetic bacterium is FERMBP-7434 strain, the above arrangement can provide stably a healthy food that has an excellent
function for maintaining and recovering health.
The healthy food of the present invention is arranged to have a metabolic product obtained by incubating the photosynthetic bacterium so that a viscous material is produced from the photosynthetic bacterium.
Therefore, because the healthy food has the metabolic product obtained by incubating the photosynthetic bacterium so that a viscous material is produced from the photosynthetic bacterium, the above arrangement can provide the healthy food, administration of which can maintain or recover the health.



We claim:
1. A method of producing a healthy food, comprising the steps of: incubating a photosynthetic bacterium in the presence of light so that a viscous material is produced from the photosynthetic bacterium and obtaining a metabolic product from the photosynthetic bacteria, the photosynthetic bacteria being Rhodopseudomonus capsulatas, acid hydrolysis of the metabolic product giving a product having glucose contents (weight %) ranging from 0.6 to 2.6 and the acid hydroiysis of the metabolic product after being water - washed giving a product having glucose contents {weight %) ranging from 0,8 to 3.3, ribose contents (weight %) ranging from 0.2 to 1.0, rhamnose contents (weight %0 ranging from 0.4 to 2.0, and focus contents {weight %) less than 0.6.
2. The method as set forth in claim 1, wherein the photosynthetic bacteria are PERM BP-7434 strain.
3. The method as set forth in claim 1, comprising the step of: incubating the photosynthetic bacteria together with lactic acid.
4. The method as set forth in claim 1, wherein: the metabolic product contains bacteriochlorophyll in a range between 0.2 and 0.3 (weight %)
5. The method as set forth in claim 1, wherein: the metabolic product contains corticoid materials in a range between 0.2 and 7.5{|j mol/g)
6. The method as set forth in claim 1, wherein: acid hydrolysis of the metabolic product before being water-washed gives a product having

glucose contents (weight %) ranging from 3.5 to 6.5, ribose contents {weight%) ranging from 0,4 to 0.1, rhamnose contents (weight %) ranging from 1.2 to 3.0, and fucose contents (weight %) ranging from 0.8 to 2.4.
7. The method as set forth in claim 1, wherein: acid hydrolysis of the metabolic product after being water - washed gives a product having glucose contents (weight%) ranging from 1.0 to 3.0, ribose contents (weight %) ranging from 0.3 to 0.9 rhamnose contents (weight %) ranging from 0.5 to 1.6, and fucose contents (weight %} of 0.5 or less.

Documents:

in-pct-2001-1601-che abstract duplicate.pdf

in-pct-2001-1601-che abstract.pdf

in-pct-2001-1601-che claims duplicate.pdf

in-pct-2001-1601-che claims.pdf

in-pct-2001-1601-che correspondence others.pdf

in-pct-2001-1601-che correspondence po.pdf

in-pct-2001-1601-che description (complete) duplicate.pdf

in-pct-2001-1601-che description (complete).pdf

in-pct-2001-1601-che drawing duplicate.pdf

in-pct-2001-1601-che drawing.pdf

in-pct-2001-1601-che form-1.pdf

in-pct-2001-1601-che form-19.pdf

in-pct-2001-1601-che form-3.pdf

in-pct-2001-1601-che others-1.pdf

in-pct-2001-1601-che others.pdf

in-pct-2001-1601-che pct.pdf


Patent Number 201073
Indian Patent Application Number IN/PCT/2001/1601/CHE
PG Journal Number 08/2007
Publication Date 23-Feb-2007
Grant Date 26-Jun-2006
Date of Filing 16-Nov-2001
Name of Patentee M/S. BIOCHEM INDUSTRIAL CO., LTD
Applicant Address 2-25-D-407, Wadayamadori 1-chome, Hyogo-ku Kobe-shi, Hyogo 652-0884
Inventors:
# Inventor's Name Inventor's Address
1 NA NA
PCT International Classification Number C12N 1/20
PCT International Application Number PCT/JP2001/001100
PCT International Filing date 2001-02-15
PCT Conventions:
# PCT Application Number Date of Convention Priority Country
1 2000-40218 2000-02-17 Japan