Title of Invention	A PROCESS FOR PREPARING A 4-[PHENYL-PIPERIDIN-4-YLIDENE-METHYL)-BENZAMIDE DERIVATIVES
Abstract	. A process for preparing a compound of formula I, wherein PG is a urethane protecting group such as Boc or CBZ or a benzyl or substituted benzyl protecting group, such as 2,4-dimethoxybenzyl, with 3-aminophenyl boronic acid, using a palladium catalyst, e.g. Pd(PPh3)4, in the presence of a base, e.g. Na2C03, to give the compounds of general formula III, -26-

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FORM 2 THE PATENTS ACT 1970 [39 OF 1970] COMPLETE SPECIFICATION [See Section 10; rule 13] "A PROCESS FOR PREPARING A 4-[PHENYL-PIPERIDIN-4-YLIDENE-METHYL)-BENZAMIDE DERIVATIVES" AstraZeneca AB, a Swedish company of S-151 85 Sodertalje, Sweden, The following specification particularly describes the nature of the invention and the manner in which it is to be performed :- Field of the Invention The present invention is directed to a process for preparing a compound and more particularly to novel compounds, to a process for their preparation, their use and pharmaceutical compositions comprising the novel compounds. The novel compounds are useful in therapy, and in particular for the treatment of pain, anxiety and functional gastrointestinal disorders. Background of the Invention The 5 receptor has been identified as having a role in many bodily functions such as circulatory and pain systems. Ligands for the 5 receptor may therefore find potential use as analgesics, and/or as antihypertensive agents. Ligands for the 8 receptor have also been shown to possess immunomodulatory activities. The identification of at least three different populations of opioid receptors (µ, δ and K) is now well established and all three axe apparent in both central and peripheral nervous systems of many species including man. Analgesia has been observed in various animal models when one or more of these receptors has been activated. With few exceptions, currently available selective opioid 8 ligands are peptidic in nature and are unsuitable for administration by systemic routes. One example of a non-peptidic 5-agonist is SNC80 (BibkyEJ. et al, Journal of Pharmacology and Experimental Therapeutics, 273(1), pp. 359-366 (1995)). Thereis however still a need for selective δ-agonists having not only improved selectivity, but also an improved side-effect profile- Thus, the problem underlymg the present invention was to find new analgesics having improved analgesic effects, but also with an improved side-effect profile over current µ agonists, as well as having improved systemic efficacy. . 2 Analgesics that have been identified and are existing in the prior art have many disadvantages in that they suffer from poor pharmacokinetics and are not analgesic when administered by systemic routes. Also, it has been documented that preferred 8 agonist compounds, described within the prior art, show significant convulsive effects when administered systemically. We have now found certain compounds that exhibit surprisingly improved properties, i.a. improved 6-agonist potency, in vivo potency, pharmacokinetic, bioavailability, in vitro stability and/or lower toxicity. Outline of the invention The novel compounds according to the present invention are defined by the formula I wherein R is selected from any one of (i) phenyl; (ii) pyridinyl - 3 - (iii) thienyl (iv) furanyl (v) imidazoly] (vi) fcriazolyl (vii) pyrrolyl (viii) thiazolyl 4 (ix) pyridyl-N-oxide where each R phenyl ring and R heteroaromatic ring may optionally and independently be further substituted by 1, 2 or 3 substituents independently selected from straight and branched C1-C6 alkyl, NO2, CF3, C1-C6 alkoxy, chloro, fluoro, bromo, and iodo. The substitutions on the phenyl ring and on the heteroaromatic ring may take place in any position on said ring systems; A further embodiment of the present invention is a compound according to figure I wherein R is as defined above and each R phenyl ring and R heteroaromatic ring may independently be further substituted by a methyl group A further embodiment of the present invention is a compound according to figure I wherein R is phenyl, pyrrolyl, pyridinyl, thienyl or furanyl, optionally with 1 or 2 of the preferred substituents on the R phenyl or R heteroaromatic ring. Another embodiment of the present invention is a compound according to figure I wherein R is phenyl, pyrrolyl or pyridinyl, optionally with 1 or 2 of the preferred substituents on the R phenyl or R heteroaromatic ring. Another embodiment of the present invention is a compound according to figure I wherein R is thienyl or furanyl, optionally with 1 or 2 of the preferred substituents on the R heteroaromatic ring. When the R phenyl ring and the R heteroaromatic ring(s) are substituted, the preferred substituents are independently selected from anyone of CF3, methyl, iodo, bromo, fluoro and chloro. 5 Reaction step g in Scheme 1, vide infra, is performed by reacting an intermediate compound of the general formula II wherein PG is a urethane or urethane protecting group, such as Boc and CBZ or benzyl or substituted benzyl protecting group, such as 2,4-dimethoxybenzyl, with 3-aminophenyl boronic acid, using a palladium catalyst, e.g. Pd(PPh.3)4, in the presence of a base, e.g. Na2C03, to give the compounds of general formula III, which is thereafter deprotected, under standard conditions and alkylated under reductive conditions with a compound of the general formula R -CHO to give compounds of the general formula I. The novel compounds of the present invention are useful in therapy, especially for the treatment of various pain conditions such as chronic pain, neuropathic pain, acute pain, cancer pain, pain caused by rheumatoid arthritis, migraine, visceral pain etc. This list should however not be interpreted as exhaustive. -6- Compounds of the invention are useful as immunomodulators, especially for autoimmune diseases, such as arthritis, for skin grafts, organ transplants and similar surgical needs, for collagen diseases, various allergies, for use as anti-tumour agents and anti viral agents. Compounds of the invention are useful in disease states where degeneration or dysfunction of opioid receptors is present or implicated in that paradigm. This may involve the use of isotopically labelled versions of the compounds of the invention in diagnostic techniques and imaging applications such as positron emission tomography (PET). Compounds of the invention are useful for the treatment of diarrhoea, depression, anxiety and stress-related disorders such as post-traumatic stress disorders, panic disorder, generalized anxiety disorder, social phobia, and obsessive compulsive disorder; urinary incontinence, various mental illnesses, cough, lung oedema, various gastro-intestinal disorders, e.g. constipation, functional gastrointestinal disorders such as Irritable Bowel Syndrome and Functional Dyspepsia, Parkinson's disease and other motor disorders, traumatic brain injury, stroke, cardioprotection following miocardial infarction, spinal injury and drug addiction, including the treatment of alcohol, nicotine, opioid and other drug abuse and for disorders of the sympathetic nervous system for example hypertension. Compounds of the invention are useful as an analgesic agent for use during general anaesthesia and monitored anaesthesia care. Combinations of agents with different properties are often used to achieve a balance of effects needed to maintain the anaesthetic state (eg. amnesia, analgesia, muscle relaxation and sedation). Included in this combination are inhaled anaesthetics, hypnotics, anxiolytics, neuromuscular blockers and opioids. Also within the scope of the invention is the use of any of the compounds according to the formula I above, for the manufacture of a medicament for the treatment of any of the conditions discussed above. 7 A. further aspect of the invention is a method for the treatment of a subject suffering from any of the conditions discussed above, whereby an effective amount of a compound according to the formula I above, is administered to a patient in need of such treatment. A further aspect of the present invention is intermediates of the general formula II and III, wherein PG is a urethane protecting group such as Boc or CBZ, or a benzyl or substituted benzyl protecting group, such as 2,4-dimethoxybenzyl. Methods of preparation EXAMPLES The invention will now be described in more detail by the following Schemes and Examples, which are not to be construed as limiting the invention. Example 1: N,N-diethyl-4-(3-aminolphenvl-piDeridin-4-vlidene-methyl)-benzamide (compound 7) (i) Preparation of 4-(4-Methoxvcarbonvl-ben2vlidene)-piperidine-i-carboxylic acid tert- butyl ester. A mixture of starting material 1 (11.2 g, 49 mmol) and trimethyl phosphite (25 mL) was refluxed under N2 for 5 hrs. Excess trimethyl phosphite was removed by co-distillation with toluene to give compound 2 in quantitative yield: (ii) 4-(4-Methoxycarbonyl-benzylidene)-piperidine-l-carboxylic acid terf-butyl ester (compound 3) To a solution of 2 in dry THF (200 mL) was added dropwise lithium diisopropylamide (32.7 mL 1.5 M in hexanes, 49 mmol) at -78 °C. The reaction mixture was then allowed to warm to room temperature prior to addition of N-tert-butoxycarbonyl-4-piperidone (9.76 g, 49 mmol in 100 mL dry THF). After 12 hrs, the reaction mixture was quenched with water (300 mL) and extracted with ethyl acetate (3 x 300 mL). The combined organic phases were dried over MgS04 and evaporated to give a crude product, which was purified by flash to provide 3 as a white solid (5.64 g, 35%): (iii) Preparation of 4-Bromo-4-[bromo-(4-methoxycarbonyl-phenyl)-methyl]':piperidine-l-carboxylic acid tert-buryl ester (compound 4) -10- To a mixture of 3 (5.2 g, 16 mmol) and K2CO3 (1.0 g) in dry dichloromethane (200 mL) was added a solution of bromine (2.9 g, 18 mmol) in 30 mL CH2CI2 at 0 °C. after 1.5 hrs at room temperature, the solution after filtration of K2CO3 was condensed. The residue was then dissolved in ethyl acetate (200 mL), washed with water (200 mL), 0.5 MHCl (200 mL) and brine (200 mL), and dried over MgSO4. Removal of solvents provided a crude product, which was recrystallized from methanol to give 4 as a white solid (6.07 g, 78%): (iv) 4-fbromo-f4-carboxv-t)henv])-methvlene1-piperidine-l-carboxvlic acid tert-butyl ester (compound 5) A solution of 4 (5.4 g 11 mmol) in methanol (300 mL) and 2.0 M NaOH (100 mL) was heated at 40 °C for 3 hrs. The solid.was collected by filtration, and dried overnight under vacuum. The dry salt was dissolved in 40% acetonitrile/water, and was adjusted to pH 2 using concentrated HC1. Product 5 (3.8 g, 87%) was isolated as a white powder by filtration: (v) 4-rbromo-(4-diethvlcarbamoyl-phenvl')-methvlenel-piperidme-l-carboxvlic acid tert-butyl ester (compound 6) To a solution of compound 5 (1.0 g, 2.5 mmol) in dry dichloromethane (10 mL) at - 20 °C was added isoburylchloroformate (450 mg, 3.3 mmol). After 20 min at -20 °C diethylamine (4 mL) was added and the reaction was allowed to warm to room temperature. After 1.5 hrs -11- the solvents were evaporated and the residue was partitioned between ethyl acetate and water. The organic phase was washed with brine and dried over MgSO4. Removal of solvents provided a crude product, which was purified by flash chromatography to give compound 6 as"white needles (800 mg, 73%): IR(NaCl) 30517297571694,163331416, 1281,1168,1115 cm"1; !H NMR (CDC13) δ 1.13 (br, 3H, CH3), 1.22 (br, 3H, CH3), 1.44 (s,. 9H, 'Bu); 2.22 (t, J=5.5 Hz, 2H), 2.62 (t, J=5.5 Hz, 2H), 3.33 (m, 4H), 3.55 (m, 4H), 1.31 (d,7=8.0 Hz, 2H, Ar-H), 7.36 (d, J=8.0 Hz, 2H, Ar-H); 13C NMR (CDC13) δ 12.71,14.13,28.3,31.5,34.2, 39.1, 43.2, 79.7,115.9,126.3, 129.3,136.8,137.1,140.6,154.6, 170.5. (vi) Preparation of N,N-diethvl-4-(3'hydroxvlp'henvl-piperidin-4-vlidene-methvlV" benzamide (compound 7) To a flask containing vinyl bromide (6) (8.5g, 18.9mM) is added xylene (120mL), ethanol (80mL) and 3-aminophenylboronic acid (1.5eq). The solution is degassed for 30 minutes, then aqueous sodium carbonate (2N, 29mL, 3.0 eq, degassed for 30 minutes) is added via cannula. Then palladium tetrakistriphenylphosphine (0.075eq) is added. The reaction mixture is degassed for 10 minutes and heated to 80°C overnight. The reaction is cooled, diluted with water and filtered through a pad of diatomaceous earth.. The organics are removed and the aqueous extracted with ether (2 x 50mL). The combined organic extract is dried with anhydrous magnesium sulfate, filtered and concentrated. The residue was used crude for the next transformations. Example 2: N,N-diemvl-4-(3-aminophenyl-N-benzvl-piperidm--4-vlidene.-methvl) benzamide (compound 9) (i) Preparation of N,N-diemvl-4-( bromo-A^benzvl-piperidin-4-vlidene-methvl')-benzamide (compound 8) Compound 6, prepared in Example l(v) above (6.122 g, 13.4 mmoL), was treated with TFA (13 mL) in dichloromethane (80 mL) at room temperature. After 2 h, the reaction mixture was washed with 2M sodium hydroxide (25mL) and the organic layer was separated. The organic layer was dried (MgSO), filtered and concentrated. The residue -12- was dissolved in dichloromethane (120mL), cooled to 0 C and benzyl bromide (1.8mL, 15.1mmol) and triethylamine (5.7mL, 41 .Ommol) were added. The reaction was gradually warmed to room temperature and after 20 hours the reaction was washed with water (1 x lOOmL). The organic layer was dried (MgS04), filtered and cohcehtrated. Purification by flash chromatography, eluting 50 to 60% ethyl acetate in hexanes gave 3.80g of product (64% yield). 1HNMR (CDCLj) 8 1.13 (br, 3H, CH3), 1.23 (br, 3H, CH3), 2.28 (m, 2H), 2.37 (m, 2H), 2.55 (m, 2H), 2.69 (m, 2H), 3.27 (m, 2H), 3.53 (br, 4H), 7.20-7.40 (m, 9H, Ar-H). rii)N,N-diethyl-4-(3-aminophenvl-N-benzvlrpiperidin-4-vlidene-methvlVbenzamide (compound 9) To a flask containing 8.5g of vinyl bromide (8) was added 120mL xylene, 80mL ethanol and 3.96g 3-aminophenyl boronic acid (1.5eq). The solution was degassed for 30 minutes then 29.0mL 2N sodium carbonate (3.0eq) (degassed for 30 minutes) was added via cannula. Then palladium tetrakistriphenylphosphine (1.67g, 0.075eq) was added. The reaction mixture was degassed for 10 minutes, then warmed to 80°C for 17 hours. The • mixture was then cooled, diluted with water and filtered through diatomaceous earth. The organics were removed and the aqueous extracted with ether (2X). The combined organics were dried with anhydrous magnesium sulfate, filtered and concentrated. Residue was purified by flash chromatography eluting with 2% methanol to 4% methanol in dichloromethane. The product (8.14g, 93%) was obtained as as orange foam. Residue was dissolved in 80mL ether and 40mL HCl/ether was added. The suspension was concentrated after 30 minutes and solid was dried under high vacuum. Examples 3-9 Additional Examples 3-9 were prepared by following the general synthetic procedure below. To a solution of compound 7 in dry tetrahydrofuran (THF) is added the aldehyde (1-1.5eq), followed by sodium triacetoxyborohydride (1 -1,6eq). The reaction is stirred at room temperature under a nitrogen atmosphere, for an extended period of time (6-48 hours) to ensure complete reaction. The reaction mixture is then subjected to a standard work-up procedure and standard purification. The amount of THF is not crucial. An amount corresponding to about 30mL per gram of amine is preferred. The procedure described below for Example 3 is typical. Example 3: To a solution of amine 7 (540 mg, 1.48 mmol, 1.0 equiv.) in tetrahydrofuran (20 ml) at room temperature was added 2-pyridine carboxaldehyde (170 µl, 1.38 mmol, 1.2 equiv.). After stirring for 10 minutes sodium triacetoxyborohydride (410 rag, 1.93 mmol, 1.3 equiv.) was added to the solution. After stirring overnight, the reaction mixture was diluted with dichloromethane (10 ml) and 2M aqueous sodium hydroxide solution (15 ml). The phases were separated and the aqueous phase is back-extracted with dichloromethane three -14- times (15 ml). The organic phases were combined, dried with sodium sulfate, filtered and • concentrated under reduced pressure. The crude product was purified by reverse phase - preparative HPLC (gradient :10% to 50% B in A, A;01%TFA.mwater; B: 0.1%TFA in acetonitrile). The fraction was concentrated under reduced pressure and neutralized to pH=l1 with 2M aqueous sodium hydroxyde solution. The mixture is then extracted twice with ethyl acetate (30 ml). The organic phases are combined, dried with sodium sulfate, filtered. To this mixture was added IM HCl solution in diethyl ether (4 ml, ca. 3.5 equiv.). The resulting mixture was then concentrated under reduced pressure. The white solid was triturated with diethyl ether and concentrated under reduced pressure to yield 135 mg, (19% yield) Additional examples were prepared analogously. Analytical data for the synthetic Examples is shown in Table 1 below. -15- Table 1: Analytical data for synthetic Examples. Pharmaceutical compositions The novel compounds according to the present invention may be administered orally, sublingually, intramuscularly, subcutaneously, topically, intranasally, intraperitoneally, intrathoracially, intravenously, epidurally, intrathecally, intracerebroventricularly and by injection into the joints. A preferred route of administration is orally, intravenously or intramuscularly. The dosage will depend on the route of administration, the severity of the disease, age and weight of the patient and other factors normally considered by the attending physician, when determining the individual regimen and dosage level as the most appropriate for a particular patient. For preparing pharmaceutical compositions from the compounds of this invention, inert, pharmaceutically acceptable carriers can be either solid or liquid. Solid form preparations include powders, tablets, dispersible granules, capsules, cachets, and suppositories. A solid carrier can be one or more substances which may also act as diluents, flavoring agents, solubilizers, lubricants, suspending agents, binders, or tablet disintegrating agents; it can also be an encapsulating material. In powders, the carrier is a finely divided solid which is in a mixture with the finely divided active component. In tablets, the active component is mixed with the carrier having the necessary binding properties in suitable proportions and compacted in the shape and size desired. For preparing suppository compositions, a low-melting wax such as a mixture of fatty acid glycerides and cocoa butter is first melted and the active ingredient is dispersed therein by, for example, stirring. The molten homogeneous mixture is then poured into convenient sized molds and allowed to cool and solidify. Suitable carriers are magnesium carbonate, magnesium stearate, talc, lactose, sugar, pectin, dextrin, starch, tragacanth, methyl cellulose, sodium carboxymethyl cellulose, a low-melting wax, cocoa butter, and the like. Salts include, but are not limited to, pharmaceutically acceptable salts. Examples of pharmaceutically acceptable salts within the scope of the present invention include: acetate, benzenesulfonate, benzoate, bicarbonate, bitartrate, bromide, calcium acetate, camsylate, carbonate, chloride, citrate, dihydrochloride, edetate, edisylate, estolate, esylate, fumarate, glucaptate, gluconate, glutamate, glycollylarsanilate, hexylresorcinate, hydrabamine, hydrobromide, hydrochloride, hydroxynaphthoate, isethionate, lactate, lactobionate, malate, maleate, mandelate, mesylate, methylbromide, methylnitrate, methylsulfate, mucate, napsylate, nitrate, pamoate (embonate), pantothenate, phosphate/diphosphate, polygalacturonate, salicylate, stearate, subacetate, succinate, sulfate, tannate, tartrate, teoclate, triethiodide, and benzathine. Examples of pharmaceutically unacceptable salts within the scope of the present invention include: hydroiodide, perchlorate, tetrafiuoroborate. Pharmaceutically unacceptable salts could be of use because of their advantageous physical and/or chemical properties, such as crystallinity. Preferred pharmaceutically acceptable salts are hydrochlorides, sulfates and bitartrates. The hydrochloride and sulfate salts are particularly preferred. The term composition is intended to include the formulation of the active component with encapsulating material as a carrier providing a capsule in which the active component (with -18- or without other carriers) is surrounded by a carrier which is thus in association with it. Similarly, cachets are included. Tablets, powders, cachets, and capsules can be used as solid dosage forms suitable for oral administration. Liquid from compositions include solutions, suspensions, and emulsions. Sterile water or water-propylene glycol solutions of the active compounds may be mentioned as an example of liquid preparations suitable for parenteral administration. Liquid compositions can also be formulated in solution in aqueous polyethylene glycol solution. Aqueous solutions fororal administration can be prepared by dissolving the active component in water and adding suitable colorants, flavoring agents, stabilizers, and thickening agents as desired. Aqueous suspensions for oral use can be made by dispersing the finely divided active component in water together with a viscous material such as natural synthetic gums, resins, methyl cellulose, sodium carboxymethyl cellulose, and other suspending agents known to the pharmaceutical formulation art Preferably the pharmaceutical compositions is in unit dosage form. In such form, the composition is divided into unit doses containing appropriate quantities of the active component. The unit dosage form can be a packaged preparation, the package containing discrete quantities of the preparations, for example, packeted tablets, capsules, and powders in vials or ampoules. The unit dosage form can also be a capsule, cachet, or tablet itself, or it can be the appropriate number of any of these packaged forms. BIOLOGICAL EVALUATION In vitro model Cell culture A. Human 293 S cells expressing cloned human µ, 6, and K receptors and neomycin resistance were grown in suspension at 37°C and 5% C02 in shaker flasks containing calcium-free DMEM10% FBS, 5% BCS, 0.1% Pluronic F-68, and 600 ug/ml geneticin. -19- B. Mouse and rat brains were weighed and rinsed in ice-cold PBS (containing 2.5mM EDTA, pH 7.4). The brains .were, homogenized with a ppjyfron for 15 sec (mouse) or 30 sec (rat) in ice-cold lysis buffer (50mM Tris, pH 7.0,2.5mM EDTA, with phenylmethylsulfonyl fluoride added just prior us.e to 0.5MmM from a 0.5M stock in DMSO.-ethanoI). Membrane preparation Cells were pelleted and resuspended in lysis buffer (50 mM Tris, pH 7.0, 2.5 mM EDTA, with PMSF added just prior to use to 0.1 mM from a 0.1 M stock in ethanol), incubated on ice for 15 min, then homogenized with a polytron for 30 sec. The suspension was spun at lOOOg (max) for 10 min at 4°C. The supernatant was saved on ice and the pellets resuspended and spun as before. The superaatants from both spins were combined and spun at 46,000 g(max) for 30 min. The pellets were resuspended in cold Tris buffer (50 mM Tris/Cl, pH 7.0) and spun again. The final pellets were resuspended in membrane buffer ( 50 mM Tris, 0.32 M sucrose, pH 7.0). Aliquots (1 ml) in polypropylene tubes were frozen in dry ice/ethanol and stored at -70°C until use. The protein concentrations were determined by a modified Lowry assay with SDS. Binding assays Membranes were thawed at 37°C, cooled on ice, passed 3 times through a 25-gauge needle, and diluted into binding buffer (50 mM Tris, 3 mM MgCl2, 1 mg/ml BSA (Sigma A-7888), pH 7.4, which was stored at 4°C after filtration through a 0.22 m filter, and to which had been freshly added 5 ug/ml aprotinin, 10 uM bestatin, 10 µM diprotin A, no DTT). Aliquots of 100 ul were added to iced 12x75 mm polypropylene tubes containing 100 µl of the appropriate radioligand and 100 µl of test compound at various concentrations. Total (TB) and nonspecific (NS) binding were determined in the absence and presence of 10 uM naloxone respectively. The tubes were vortexed and incubated at 25°C for 60-75 min, after which time the contents are rapidly vacuum-filtered and washed with about 12 ml/tube iced wash buffer (50 mM Tris, pH 7.0, 3 mM MgCl2) through GF/B -20- filters (Whatman) presoaked for at least 2h in 0.1 % polyethyleneimine. The radioactivity (dpm) retained on the filters was measured with a beta counter after soaking the filters for at least I2h in mmivialscontaining 6-7 ml scintillation fluid If the assay is set up in 96-place deep well plates, the filtration is over 96-place PEI-soaked unifilters, which were washed with 3 x 1 ml wash buffer, and dried in an oven at 55°C for 2h. The filter plates were counted in a TopCount (Packard) after adding 50 µl MS-20 scintillation fluid/well. Functional Assays The agonist activity of, the compounds is measured by determining the degree to which the compounds receptor complex activates the binding of GTP to G-proteins to which the receptors are coupled. In the GTP binding assay, GTP[γ]35S is combined with test compounds and membranes from HEK:293S cells expressing the cloned human opioid receptors or from homogenised rat and mouse brain. Agonists stimulate GTP[γ]35S binding in these membranes. The EC50 and Emax values of compounds are determined from dose-response curves. Right shifts of the dose response curve by the delta antagonist naltrindole are performed to verify that agonist activity is mediated through delta receptors. Procedure for rat brain GTP Rat brain membranes are thawed at 37°C, passed 3 times through a 25-gauge blunt-end needle and diluted in the GTPyS binding (50 mM Hepes, 20 mM NaOH, 100 mM NaCI, 1 mM EDTA, 5 mM MgCl2,pH 7.4, Add fresh: 1 mM DTT, 0.1% BSA). 120µM GDP final is added membranes dilutions. The EC50 and Emax of compounds are evaluated from 10-point dose-response curves done in 300uf with the appropriate amount of membrane protein (20ng/well) and 100000-130000 dpm of GTPγ35S per well (0.11 -0.14nM).The basal and maximal stimulated binding are determined in absence and presence of 3µM SNC-80 Data analysis The specific binding (SB) was calculated as TB-NS, and the SB in the presence of various test compounds was expressed as percentage of control SB. Values of IC50 and Hill -21- coefficient (nH) for ligands in displacing specifically bound radioligand were calculated from logit plots or curve fitting programs such as Ligand, GraphPad Prism, SigmaPlot, or RecepiorFit. Values of Ki were calculated from the.Cheng-Prussoff equation. Mean ± S.E.M. values of IC50, Ki and nH were reported for ligands tested in at least three displacement curves. Biological activity of the compounds of the present invention is indicated in Table 2. Table 2: Biological Data. Ex. HDELTA (nM) RAT BRAIN (nM) MOUSE BRAIN (nM) IC50 EC50 %EMax EC50 %EMax EC50 %EMax 1-8 0.22-2.18 0.55-13.4 93-106 5.57-106 . 67-155 9.08-207.5 73-144 Receptor Saturation Experiments Radioligand Kδ values were determined by performing the binding assays on cell membranes with the appropriate radioligands at concentrations ranging from 0.2 to 5 times me estimated Kδ (up to 10 times if amounts of radioligand required are feasible). The specific radioligand binding was expressed as pmole/mg membrane protein. Values of Kδ and Bmax from individual experiments were obtained from nonlinear fits of specifically bound (B) vs. nM free (F) radioligand from individual according to a one-site model. Determination Of Mechano-Allodvnia Using Von Frey Testing Testing was performed between 08:00 and 16:00h using the method described by Chaplan et al. (1994). Rats were placed in Plexiglas cages on top of a wire mesh bottom which allowed access to the paw, and were left to habituate for 10-15 min. The area tested was the mid-plantar left hind paw, avoiding the less sensitive foot pads. The paw was touched with a series of 8 Von Frey hairs with logarithmically incremental stiffness (0.41, 0.69, 1.20, 2.04, 3.63, 5.50, 8.51, and 15.14 grams; Stoeltmg, III, USA). The von Frey hair was applied from underneath the mesh floor perpendicular to the plantar surface with sufficient -22- force to cause a slight buckling against the paw, and held for approximately 6-8 seconds. A positive response was noted if the paw was sharply withdrawn. Flinching immediately upon removal-of-the hair was also considered a positive response. Ambulation was considered an ambiguous response, and in such cases the stimulus was repeated. Testing Protocol The animals were tested on postoperative day 1 for the FCA-treated group. The 50% withdrawal threshold was determined using the up-down method of Dixon (1980). Testing was started with the 2.04 g hair, in the middle of the series. Stimuli were always presented in a consecutive way, whether aspending or descending. In the absence of a paw withdrawal response to the initially selected hair, a stronger stimulus Was presented; in the event of paw withdrawal, the next weaker stimulus was chosen. Optimal threshold calculation by this method requires 6 responses in the immediate vicinity of the 50% threshold, and counting of these 6 responses: began when the first change in response occurred, e.g. the threshold was first crossed. In cases where thresholds fell outside the range of stimuli, values of 15.14 (normal sensitivity) or 0.41 (maximally allodynic) were respectively assigned. The resulting partem of positive and negative responses was tabulated using the convention, X = no withdrawal; O = withdrawal, and the 50% withdrawal threshold was interpolated using the formula: 50% g threshold = 10(xf + kS) / 10,000 where Xf - value of the last von Frey hair used (log units); k = tabular value (from Chaplan et al. (1994)) for the pattern of positive / negative responses; and 5 = mean difference between stimuli (log units). Here 5 = 0.224. Von Frey thresholds were converted to percent of maximum possible effect (% MPE), according to Chaplan et al. 1994. The following equation was used to compute % MPE: % MPE = Drug treated threshold f (g) - allodvnia threshold (p) X 300 Control threshold (g) - allodynia threshold (g) -23- Administration Of Test Substance Rats were injected (subcutaneously, intraperitoneally, intravenously or orally) with a test substance prior to von Frey testing, the time between administration of test compound and the von Frey test varied depending upon the nature of the test compound. Writhing Test Acetic acid will bring abdominal contractions when administered intraperitoneally in mice. These will then extend their body in a typical pattern. When analgesic drugs are administered, this described movement is less frequently observed and the drug selected as a potential good candidate. A complete and typical Writhing reflex is considered only when the following elements are present: the animal is not in movement; the lower back is slightly depressed; the plantar aspect of both paws is observable. In this assay, compounds of the present invention demonstrate significant inhibition of writhing responses after oral dosing of l-100µmol/kg. (i) Solutions preparation Acetic acid (AcOH): 120 µL of Acetic Acid is added to 19.88 ml of distilled water in order to obtain a final volume of 20 ml with a final concentration of 0.6% AcOH. The solution is then mixed (vortex) and ready for injection. Compound (drug"): Each compound is prepared and dissolved in the most suitable vehicle according to standard procedures. (ii) Solutions administration The compound (drug) is administered orally, intraperitoneally (i.p.), subcutaneously (s.c.) or intravenously (i.v.)) at 10 ml/kg (considering the average mice body weight) 20, 30 or 40 minutes (according to the class of compound and its characteristics) prior to testing. When the compound is delivered centrally: Intraventricularly (i.c.v.) or intrathecally (i.t.) a volume of 5 µL is administered. -24- The AcOH is administered intraperitoneally (i.p.) in two sites at 10 ml/kg (considering the average mice body weight) -immediately prior to testing. — (iii) Testing The animal (mouse) is observed for a period of 20 minutes and the number of occasions (Writhing reflex) noted and compiled at the end of the experiment. Mice are kept in individual "shoe box" cages with contact bedding. A total of 4 mice are usually observed at the same time: one control and three doses of drug. For the anxiety and anxiety-like indications, efficacy has been established in the geller-seifter conflict test in the rat. For the functional gastrointestina disorder indication, efficacy can be established in the assay described by Coutinho SV et al, in American Journal of Physiology - Gastrointestinal & Liver Physiology. 282(2):G307-16, 2002 Feb, in the rat. . -25- CLAIM:- comprising reacting a compound of the general formula II 1. A process for preparing a compound of formula I, wherein PG is a urethane protecting group such as Boc or CBZ or a benzyl or substituted benzyl protecting group, such as 2,4-dimethoxybenzyl, with 3-aminophenyl boronic acid, using a palladium catalyst, e.g. Pd(PPh3)4, in the presence of a base, e.g. Na2C03, to give the compounds of general formula III, -26- which is thereafter deprotected, under standard conditions and, alkylated under reductive conditions with a compound of the general formula R1-CHO to give compounds of the general formula wherein R1 is selected from phenyl; pyridinyl; thienyl; furanyl; imidazolyl; triazolyl; pyrrolyl; thiazolyl; pyridyl-N-oxide, wherein each R1 phenyl ring or R1 heteroaromatic ring may independently be further substituted by 1, 2 or 3 substituents independently selected from straight and branched C1-C6 alkyl, NO2, CF3, C1-C6 alkoxy, chloro, fluoro, bromo, and iodo, as well as salts thereof. Dated this 3rd day November, 2003. -27-

Documents:

01010-mumnp-2003-cancelled page(6-5-2004).pdf

01010-mumnp-2003-claim(granted)-(6-5-2004).doc

01010-mumnp-2003-claim(granted)-(6-5-2004).pdf

01010-mumnp-2003-correspondence(4-1-2005).pdf

01010-mumnp-2003-correspondence(ipo)-(2-4-2007).pdf

01010-mumnp-2003-description(granted)-(6-5-2004).doc

01010-mumnp-2003-form 19(3-11-2003).pdf

01010-mumnp-2003-form 1a(3-11-2003).pdf

01010-mumnp-2003-form 1a(5-1-2005).pdf

01010-mumnp-2003-form 2(granted)-(6-5-2004).doc

01010-mumnp-2003-form 2(granted)-(6-5-2004).pdf

01010-mumnp-2003-form 3(13-7-2004).pdf

01010-mumnp-2003-form 3(3-11-2003).pdf

01010-mumnp-2003-form 3(6-5-2004).pdf

01010-mumnp-2003-form 5(6-5-2004).pdf

01010-mumnp-2003-form-pct-ipea-409(3-11-2003).pdf

01010-mumnp-2003-form-pct-isa-210(3-11-2003).pdf

01010-mumnp-2003-petition under rule 137(6-5-2004).pdf

01010-mumnp-2003-petition under rule 138(6-5-2004).pdf

01010-mumnp-2003-power of authority(14-10-2003).pdf

1010-mumnp-2003-abstract(6-5-2004).pdf

1010-mumnp-2003-abstract(granted)-(2-4-2007).pdf

1010-mumnp-2003-claims(complete)-(3-11-2003).pdf

1010-mumnp-2003-claims(granted)-(2-4-2007).pdf

1010-mumnp-2003-correspondence(ipo)-(10-5-2007).pdf

1010-mumnp-2003-description(complete)-(3-11-2003).pdf

1010-mumnp-2003-description(granted)-(2-4-2007).pdf

1010-mumnp-2003-form 2(complete)-(3-11-2003).pdf

1010-mumnp-2003-form 2(granted)-(2-4-2007).pdf

1010-mumnp-2003-form 2(title page)-(complete)-(3-11-2003).pdf

1010-mumnp-2003-form 2(title page)-(granted)-(2-4-2007).pdf

1010-mumnp-2003-form 5(3-11-2003).pdf

1010-mumnp-2003-specification(amended)-(5-1-2005).pdf

1010-mumnp-2003-specification(amended)-(6-5-2004).pdf

1010-mumnp-2003-wo international publication report(3-11-2003).pdf