Title of Invention	A PROTOCOL FOR MICROPROPAGATION OF BAMBOO FROM EXPLANTS
Abstract	A protocol for micropropagation of bamboo from explants, which comprises in the steps of: (a) treating explants with a fungicide, and then washing for removal of the said fungicide; (b) inoculating the explants with Murashige & Skoog culture medium having a growth regulator, i.e. benzyl amino purine in concentration of 3.0 to 5.0 mg/1 to initiate shoot culture; (c) subjecting the shoots to the step of shoot multiplication in a culture environment and exposed to atleast 1600 lux light and at 22 to 24°C tempetature; and (d) subjecting the said shoots to the step of rooting in the presence of Murashige & Skoog culture medium containing a growth regulator, i.e. naphthyl acetic acid in an amount of 3.0 mg/1 and atleast 1600 lux light and 22 to 24°C temperature.

Title of Invention

A PROTOCOL FOR MICROPROPAGATION OF BAMBOO FROM EXPLANTS

Abstract

A protocol for micropropagation of bamboo from explants, which comprises in the steps of: (a) treating explants with a fungicide, and then washing for removal of the said fungicide; (b) inoculating the explants with Murashige & Skoog culture medium having a growth regulator, i.e. benzyl amino purine in concentration of 3.0 to 5.0 mg/1 to initiate shoot culture; (c) subjecting the shoots to the step of shoot multiplication in a culture environment and exposed to atleast 1600 lux light and at 22 to 24°C tempetature; and (d) subjecting the said shoots to the step of rooting in the presence of Murashige & Skoog culture medium containing a growth regulator, i.e. naphthyl acetic acid in an amount of 3.0 mg/1 and atleast 1600 lux light and 22 to 24°C temperature.

Full Text	THE PATENTS ACT. 1970 COMPLETE SPECIFICATION SECTION 10 TITLE "A protocol for micropropagation of bamboo from explants" APPLICANT DIRECTOR of Tropical Forest Research Institute, Jabalpur, M.P., India, an Indian National. The following Specification Particularly describes and ascertains the nature of this invention and the manner in which is to be performed. GRANTED 18-12-2000 FIELD OF INVENTION This invention relates to a protocol for micropropagation of bamboo from explants. PRIOR ART Protocol or process for micropropagation of teak from explants is known in the art. However, due to the importance of bamboo, the present invention ' recognizes the necessity of a protocol or process for micropropagation of bamboo from explants. OBJECTS OF THE INVENTION An object of this invention is to propose an improved process for micropropagation of bamboo from explants. Yet another object of this invention is to propose an improved process for micropropagation of bamboo from explants which is efficient. Yet another object of this invention is to propose an improved process for micropropagation of bamboo from explants which has a high multiplication rate. Further objects and advantages of this invention will be more apparent from the ensuing description; ,j DESCRIPTION OF INVENTION According to this invention there is provided a protocol for micropropagation of bamboo from explants which comprises in the steps of: a) treating the explant with a known fungicide, and then Washing for removal of said fungicide, b) inoculating the explant with a culture medium having a growth regulator to initiate shoot culture, c) subjecting the shoot to the step of shoot multiplication in a culture environment and exposed to light, d) Subjecting the shoots to the step of rooting in the presence of a culture medium containing a growth regulator.literary In accordance with the process the present invention, the explants are treated with any known funcigicide for removal of fungal infections. Any known fungicide, such as "Bavistin" may be used for this purpose. The treated explants are then washed first with water and then distilled water for removal of any fungicide that may still be adhering to the explant. Reference to explants is intended to imply matured ripened seeds and buds. Further, such explants may be bamboo species, Dendrocalamus asper, Bambusa bambos and Dendro-calmus membranaceus. The treated explants are then subjected to a second step of inoculation with a culture medium. Such a step of inoculation is carried out in an inoculation chamber or environment having a controlled temperature of 22° to 24°C and under laminar flow and presence of UV light. The treatment under lamina flow is preferred as it avoids the possibility of the culture being infected by any infection. Such a step of inoculation initiates or supports the culture. The medium consists of a known culture medium and having a regulator such as benzyl amino purine, which is a hormone present in a concentration of 3.0 to 5.0 mal The next step in the process consists in shoot multiplication. For this purpose, the shoot in the same medium is transferred to a culture room and exposed to light having an intensity of atleast 1600 in lux and prefereably between 1500 to 1800 lux and at a temperature of 22° to 24° C for a period of 14 to 18 hours. The final step consists of rooting in a culture medium MS with a plant regulator napthyal acetic acid and present in a concentration of 3.0 mal. Such a step of rooting is carried in light having an intensity of atleast 1600 in lux and prefereably between 1500 to 1800 lux at a temperature of 22 to 24°C. Such a step of rooting induces the growth of roots to the shoots for further growth upon field transfer. Table 1 shows the results of the three specific species of bamboo subjected to the protocol of the present invention. Table 1 ; Protocol for Micropropagation of Bamboo S. Name Explant Shoot Shoot Rooting Har N. Of the Initiation Multipli- Media den O. Species And Establish cation ing Ment medium Medi Rate (fold) Medium Succe succ ess 1. Dendrocala Seed& M.S+ MS 10-12 MS+ 90-95% 90% mus Axillary 3.0-5.0 3.0- 3.0 asper buds, BAP BAP NAA Bambusa Seed& MS + MS 6 MS+ 90% 90% 2. Bambos Axillary 3.0-5.0 3.0 3.0 Buds BAP BAP NAA 3. Dendrocal- Axillary MS + MS 10-12 MS + 95% 90% amus Buds 3.0 2.0 2.0 Membrana-ceus BAP BAP NAA We claim 1. A protocol for micropropagation of bamboo from explants, which comprises in the steps of: (a) treating explants with a fungicide, and then washing for removal of the said fungicide; (b) inoculating the explants with Murashige & Skoog culture medium having a growth regulator, i.e. benzyl amino purine in concentration of 3.0 to 5.0 mg/1 to initiate shoot culture; (c) subjecting the shoots to the step of shoot multiplication in a culture environment and exposed to atleast 1600 lux light and at 22 to 24°C tempetature; and (d) subjecting the said shoots to the step of rooting in the presence of Murashige & Skoog culture medium containing a growth regulator, i.e. naphthyl acetic acid in an amount of 3.0 mg/1 and atleast 1600 lux light and 22 to 24°C temperature. Date: 15th Day of December 2000 (A.K.Mandal) Director

Full Text

THE PATENTS ACT. 1970
COMPLETE
SPECIFICATION

SECTION 10
TITLE
"A protocol for micropropagation of bamboo from explants"

APPLICANT
DIRECTOR of Tropical Forest Research Institute, Jabalpur, M.P., India, an Indian
National.

The following Specification Particularly describes and ascertains the nature of this invention and the manner in which is to be performed.

GRANTED
18-12-2000

FIELD OF INVENTION
This invention relates to a protocol for micropropagation of bamboo from explants.
PRIOR ART
Protocol or process for micropropagation of teak from explants is known in the art. However, due to the importance of bamboo, the present invention
'
recognizes the necessity of a protocol or process for micropropagation of bamboo from explants.
OBJECTS OF THE INVENTION
An object of this invention is to propose an improved process for micropropagation of bamboo from explants.
Yet another object of this invention is to propose an improved process for micropropagation of bamboo from explants which is efficient.
Yet another object of this invention is to propose an improved process for micropropagation of bamboo from explants which has a high multiplication rate.
Further objects and advantages of this invention will be more apparent from the ensuing description;
,j
DESCRIPTION OF INVENTION
According to this invention there is provided a protocol for micropropagation of bamboo from explants which comprises in the steps of: a) treating the explant with a known fungicide, and then Washing for
removal of said fungicide,

b) inoculating the explant with a culture medium having a growth regulator to initiate shoot culture,
c) subjecting the shoot to the step of shoot multiplication in a culture environment and exposed to light,
d) Subjecting the shoots to the step of rooting in the presence of a culture medium containing a growth regulator.literary
In accordance with the process the present invention, the explants are treated with any known funcigicide for removal of fungal infections. Any known fungicide, such as "Bavistin" may be used for this purpose. The treated explants are then washed first with water and then distilled water for removal of any fungicide that may still be adhering to the explant.
Reference to explants is intended to imply matured ripened seeds and buds. Further, such explants may be bamboo species, Dendrocalamus asper, Bambusa bambos and Dendro-calmus membranaceus.
The treated explants are then subjected to a second step of inoculation with a culture medium. Such a step of inoculation is carried out in an inoculation chamber or environment having a controlled temperature of 22° to 24°C and under laminar flow and presence of UV light. The treatment under lamina flow is preferred as it avoids the possibility of the culture being infected by any infection. Such a step of inoculation initiates or supports the culture. The medium consists of a known culture medium and having a regulator such as benzyl amino purine, which is a hormone present in a concentration of 3.0 to 5.0 mal

The next step in the process consists in shoot multiplication. For this purpose, the shoot in the same medium is transferred to a culture room and exposed to light having an intensity of atleast 1600 in lux and prefereably between 1500 to 1800 lux and at a temperature of 22° to 24° C for a period of 14 to 18 hours.
The final step consists of rooting in a culture medium MS with a plant regulator napthyal acetic acid and present in a concentration of 3.0 mal. Such a step of rooting is carried in light having an intensity of atleast 1600 in lux and prefereably between 1500 to 1800 lux at a temperature of 22 to 24°C. Such a step of rooting induces the growth of roots to the shoots for further growth upon field transfer.
Table 1 shows the results of the three specific species of bamboo subjected to the protocol of the present invention.
Table 1 ; Protocol for Micropropagation of Bamboo

S. Name Explant Shoot Shoot Rooting Har
N. Of the Initiation Multipli- Media den
O. Species And Establish cation ing
Ment medium Medi Rate (fold) Medium Succe succ ess
1. Dendrocala Seed& M.S+ MS 10-12 MS+ 90-95% 90%
mus Axillary 3.0-5.0 3.0- 3.0
asper buds, BAP BAP NAA
Bambusa Seed& MS + MS 6 MS+ 90% 90%
2. Bambos Axillary 3.0-5.0 3.0 3.0
Buds BAP BAP NAA
3. Dendrocal- Axillary MS + MS 10-12 MS + 95% 90%
amus Buds 3.0 2.0 2.0
Membrana-ceus BAP BAP NAA

We claim
1. A protocol for micropropagation of bamboo from explants, which comprises in the steps of:
(a) treating explants with a fungicide, and then washing for removal of the said fungicide;
(b) inoculating the explants with Murashige & Skoog culture medium having a growth regulator, i.e. benzyl amino purine in concentration of 3.0 to 5.0 mg/1 to initiate shoot culture;
(c) subjecting the shoots to the step of shoot multiplication in a culture environment and exposed to atleast 1600 lux light and at 22 to 24°C tempetature; and
(d) subjecting the said shoots to the step of rooting in the presence of Murashige & Skoog culture medium containing a growth regulator, i.e. naphthyl acetic acid in an amount of 3.0 mg/1 and atleast 1600 lux light and 22 to 24°C temperature.
Date: 15th Day of December 2000
(A.K.Mandal) Director

Documents:

1137-mum-2000-cancelled page(18-12-2000).pdf

1137-mum-2000-claim(granted)-(18-12-2000).doc

1137-mum-2000-claim(granted)-(18-12-2000).pdf

1137-mum-2000-correspondence(09-01-2006).pdf

1137-mum-2000-correspondence(ipo)-(29-06-2007).pdf

1137-mum-2000-form 1(18-12-2000).pdf

1137-mum-2000-form 1(26-11-2001).pdf

1137-mum-2000-form 19(29-11-2004).pdf

1137-mum-2000-form 2(granted)-(18-12-2000).doc

1137-mum-2000-form 2(granted)-(18-12-2000).pdf

1137-mum-2000-form 3(16-12-2005).pdf

1137-mum-2000-form 3(26-11-2001).pdf

1137-mum-2000-other document(30-12-2005).pdf

1137-mum-2000-petition under rule 124(26-11-2001).pdf

1137-mum-2000-power of attorney(26-11-2001).pdf

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Patent Number

207870

Indian Patent Application Number

1137/MUM/2000

PG Journal Number

43/2008

Publication Date

24-Oct-2008

Grant Date

29-Jun-2007

Date of Filing

18-Dec-2000

Name of Patentee

TROPICAL FOREST RESEARCH INSTITUTE

Applicant Address

JABALPUR.

Inventors:

#	Inventor's Name	Inventor's Address
1	DR. ASIM KUMAR MONDAL	TROPICAL FOREST RESEARCH INSTITUTE JABALPUR.
2	FATIMA SHIRIN	TROPICAL FOREST RESEARCH INSTITUTE, JABALPUR.
3	YOGESHWAR MISHRA	TROPICAL FOREST RESEARCH INSTITUTE JABALPUR.
4	SHAMIM AKHTAR SANSARI	TROPICAL FOREST RESEARCH INSTITUTE JABALPUR.

PCT International Classification Number

A01H 5/00

PCT International Application Number

N/A

PCT International Filing date

PCT Conventions:

#	PCT Application Number	Date of Convention	Priority Country
1			NA