Title of Invention	INDOLIN OSPIROPYRAN COMPOUNDS AND METHODS FOR THEIR MANUFACTURE.
Abstract	A compound comprising the formula: wherein R1 is a C1-C4 alkyl, phenyl, benzyl, allyl, or ethoxycarbonyl ethyl, R2 and R3 are each independently methyl, ethyl, or phenyl, R4 is hydrogen, hydroxy, trichloromethyl, trifluoromethyl, formyl, C1-C4 alkyl, halogen, C1-C4 alkoxy, nitro, cyano, C1-C4 monohaloalkyl, C1-C4 alkoxycarbonyl, or an aromatic sharing the two adjacent carbon atoms with the benzene portion of the pyran ring to form a condensed aromatic ring, and x equals 1, 2, or 3.

Title of Invention

INDOLIN OSPIROPYRAN COMPOUNDS AND METHODS FOR THEIR MANUFACTURE.

Abstract

A compound comprising the formula: wherein R1 is a C1-C4 alkyl, phenyl, benzyl, allyl, or ethoxycarbonyl ethyl, R2 and R3 are each independently methyl, ethyl, or phenyl, R4 is hydrogen, hydroxy, trichloromethyl, trifluoromethyl, formyl, C1-C4 alkyl, halogen, C1-C4 alkoxy, nitro, cyano, C1-C4 monohaloalkyl, C1-C4 alkoxycarbonyl, or an aromatic sharing the two adjacent carbon atoms with the benzene portion of the pyran ring to form a condensed aromatic ring, and x equals 1, 2, or 3.

Full Text	1 INDOLINOSPIROPYRAN COMPOUNDS AND METHODS FOR THEIR MANUFACTURE Field of the Invention The present invention relates to spiropyran,compounds. In particular, the invention provides indolinospiropyran compounds and methods for their manufacture, which compounds are useful as photochromic compounds. Background of the Invention Various classes of photochromic compounds have been synthesized, and suggested for use in applications in which reversible color changes or darkening is induced by sunlight. For example, spiro(indolino)naphthopyrans and spiro(indolino)quinopyrans are described in GB Patent 2,174,711. Spiropyrans also are described in Brown, Glenn H. ed., Photochromism (New York, 1971) and Durr, Heinz and Henri Bouas-Laurent eds., Photochromism (Elsevier, 1990). Spiropyran derivatives may be the best known organic compounds showing photochromism phenomenon, but the structures of reported spiropyrans are considerably limited. Thus, a need exists both for spiropyran compounds allowing further facile modifications as well as methods for the synthesis of diverse spiropyran compounds. Description of the Invention and its Preferred Embodiments The present invention provides indolinospiropyran compounds, and particularly photochromic indolinospiropyran compounds, as well as methods for synthesizing these compounds. The indolinospiropyran compounds of the invention are substituted on the indole ring with succinimide, which substitution permits ring opening of the succinimide and modulation of the bulk and photochromic properties of the compounds. 2 In one embodiment, the invention provides a compound comprising, consisting essentially of, and consisting of the formula: wherein R1 is C1- C18 alkyl, allyl, phenyl, mono- or disubstituted phenyl, phen(C1 -C4 )alkyl, or (C1 -C4)alkoxycarDonyl (C1-C4 )alkyl, R2and R3 are each independently C1 -C4alkyl, phenyl, mono- or di-substituted phenyl, benzyl, or combined to form a cyclic ring that is cyclohexyl, norbomyl or adamantyl ring, K4 is hydrogen, hydroxy, trichloromethyl, trifluoromethyl, formyl, C1-C4 alkyl, halogen, C1- C4 alkoxy, nitro, cyano, C1-C4 monohaloalkyl, C1-C4 alkoxycarbonyl, or an aromatic sharing the two adjacent carbon atoms with the benzene portion of the pyran ring to form a condensed aromatic ring including, without limitation, naphthyl, phenanthrenyl, and quinolino, x equals 1,2, or 3 provided that when x = V, R4 may be located on any of the available carbon atoms of the benzene ring of the benzopyran moiety, preferably on the 6,7, or 8 position and when x = 2, each of the R4 may be the same or different and located at the 6 and 8 or 5 and 7 positions, preferably at the 6 and 8 positions. R1 preferably is a C1 -C4 alkyl, phenyl, benzyl allyl, or ethoxycarbonyl ethyl, R2 and R3 preferably are each independently methyl, ethyl, or phenyl and R4 preferably is C1-C4 alkyl, C1-C2 alkoxy, chloro, bromo, iodo, trifluoromethyl, or nitro. In a preferred embodiment, the invention provides a compound comprising, consisting essentially of, and consisting of the formula: 3 wherein R4 is hydrogen, hydroxy, trifluoromethyl, formyl, methyl, ethyl, methoxy, ethoxy, nitro, fluoro, chloro, bromo, or iodo, and x is 1 or 2. Because the compounds of formulae I and II contain a succinimide portion, the properties of the compounds, such as solubility, sensitivity and the like, of the invention may be manipulated by ring opening of the succinimide using any of a variety of known methods. Suitable such methods are described, for example, in 48(12) Heterocycles, 2677-2691 (1998). Formulae I and II compounds may be prepared by any convenient known method and preferably are prepared using a solid phase organic synthesis. The use of solid phase synthesis is advantageous in that it provides ease in execution of the reaction, ease in product purification, and convenient handling,of polar molecules throughout the synthetic protocol. Additionally, this approach permits use of commercially available starting materials and use of excess reactant to drive the reaction to completion and to surpass the side reactions. Key in the synthesis is the use of a polymer-supported indoline of the formula: 4 wherein R1, R2, and R3 are the same as for fonnula (I). The solid support may be selected from any of a variety of hydroxy resins. Suitable hydroxy resins include, without limitation, bydroxyroethyl polystyrene resin, Wang resin (also known as 4-hvdroxymethyl phenpxy resin or "HMP resin"), HMPA-PEGA resin (or 4-hydroxymethylphenoxyacetic acid and bisacrylamidoprop-l-yl polyethyleneglycol), HMPB-BHA resin (or4-hydroxy-3-methoxyphenoxybutyric acid benzhydrylamine), HMPB-MBHA resin (4-hydroxymethyl~3-methoxyphenoxybutyric acid-methylbenzhydrylamine), and combinations thereof. The theoretical loading of the resin may be either low (e.g.7 less than about 0.1 mmole/g) or high (e.g., greater than about 0.4 mmole/g), but for production of greater quantities of product, preferably is high, more preferably about 0.4 to about 1.5 mmole/g. Either of about 100-200 mesh or about 200-400 mesh resin may be used. Preferred resins are an about 100-200 mesh high loading hydroxymethyl polystyrene or a Wang resin. The solid support used will depend upon the reactants selected, the solvent used, and the product desired. The. resin preferably has types and quantities of functional groups that permit, efficient attachment of the reactants as well as efficient release of the product Additionally, the resin must be swellable in the solvent-used. The amount of resin used will depend on the amount of reactants used and the reaction scale desired. Generally, about 1 mg to about 100 g of resin may be used. Fonnula m compounds, may be prepared by either of two reaction schemes using.an aminoindoline compound of the following formula: 5 wherein R1, R3, and R3 are as defined for formula I. The reaction schemes for the preparation of the formula in compound using the aminoindolino compounds are as follows: 6 In Metthod A, an aminoindoline compound of formula IV, is treated with succinic anhydride under conditions suitable to form succinamic acid. More specifically, the reaction is carried out at a temperature of about 0 to about 60° C, preferably about room temperature, under an inert atmosphere including, without limitation, argon or nitrogen, for about 3 to 24 hours. The amount of reactants used will depend on the amount of product desired and typically will be about 1 mg to about 100 g, preferabry about 100 mg to about 10 g. A hydroxy resin along with diisopropyl carbodiimide ("DIC") and dimethylamino pyridine ("DMAP") then are added to the mixture to form a suspension bead. This suspension of bead is shaken under conditions suitable to carry out a coupling reaction. Suitable conditions for the reaction are a temperature of about 0 to about 60° C, preferably about room temperature, about 14 to 24 hours under an inert atmosphere. Progress of the coupling reaction may be monitored by any convenient means including, without limitation, FT-IR or single bead FT-IR. Typically, the reaction forming the mdoline loaded resin is complete after 24 hours at room temperature. In Method B, hydroxy resin is shaken with an excess of succinic anhydride under conditions suitable to carry out a coupling reaction. Suitable conditions for the reaction are a temperature of about 60 to 120° C, preferably about 70 to about 100° C, for about 10 to about 60 hours, preferably about 24 to about 48 hours. The progress of the reaction may be monitored by any convenient means and, generally, will be complete after 48 hours of refluxing. After completion of the reaction, excess succinic anhydride is washed away and the resin, now coupled with the succinic acid, is treated with an aminoindoline of formula IV along withal-hydroxy benzotriazole ("HOBT") and DIC. Conditions suitable for the treatment are a temperature of about 0 to 60° C, preferably room temperature, and an inert atmosphere. In either method, loading typically is over about 95 percent Any convenient method for testing loading may be used including, without limitation, as disclosed in 7 63(3) J. Org. Chem., 708 -718 (1998). Hexamethyidisiloxane ("HMDSO") may be used as an internal standard for purposes of 1HNMR testing. The aminoindoline compounds of formula IV_may be prepared by reduction of nitroindoline derivatives of the formula; wherein Rl, R2, and R3 are as for formula I. Suitable nitroindoline derivatives for formula V may be prepared by nitration of indoline derivatives as described in 101(8) Bull Soc. Chim.Bdg., 719-739 (1992). The indoline derivatives may be nitrated by nitric acid in cold sulfuric acid below 10° C, preferably below 7° C, over about 10 hours. In solid phase synthesis of the indolinospiropyran compounds of the invention, a resin loaded with indoline, generally about 100 mg to about 100 g, preferably about 100 mg to about 10 g, as in formula ID may be split and treated with any of a variety of salicylaldehyde derivatives under conditions suitable to form the desired indolinospiropyran compound. Suitable conditions for carrying out the synthesis are a temperature of about 50 to 120° C under an inert atmosphere for a time of about 14 to about 11 days, preferably about 14 hours to about 3 days. Suitable salicylaldehyde derivatives are of the formula: 8 wherein R4 and x are as for formula I. The solid phase synthesis proceeds as follows: The synthesis is carried out in any suitable solvent, preferably in dioxane, dimethylformamide ("DMF"), N-methylprroiidone ("NMP"), tetrahydrofiiran ("THF"), or combinations thereof. Release of the formula I compound from the solid support may be carried out in any convenient manner such as by a base-catalyzed process using any suitable base including, without limitation, piperidine, DBU, sodium methoxide, potassium tert-butyloxide. In general, about 3 to about 4 9 equivalents of salicylaidehyde derivative is used to react with about 1.1 to about 10 equivalents, preferably about 2 to about 5, more preferably about 3 to about 4 equivalents of resin loaded with indoline. The compounds of the invention are useful in any of the wide variety of applications, such as ophthalmic lenses, windshields, windows, and the like in which photochromism is useful. More specifically, an effective amount of the compounds of the invention are incorporated into or coate(Lpnto_anjophthaimic lens. windshield, window, or like article. The article into which the compound is incorporated or onto which it is coated will darken with exposure to ultra-violet light and revert to its original color or colorless condition in when not exposed to UV light at ambient temperatures. An effective amount of the compound is an amount of about 10-5 to aboutl 10-2 mol/preferably about 10""5 to about 10"3 mol/1. Methods for incorporating into or coating such articles with photochromic compounds such as those of the invention are well know in the art The invention will be clarified by considering the following, non-limiting examples. Examples Example 1 5-Nitro-1,3,3-trimethyl-indoline. 2,3,4-Trimethyl-indoline (17.3, 0.1 mole) was added to 45 ml cold sulfuric acid drop-wise at below 5° C with stirring. 7.0 g Nitric acid (1 mole) in 18 mL sulfuric acid then were added drop-wise with stirring over 1 hr while keeping the. temperature below 7° C. Stirring was continued for 3 hrs at 7° C. After allowing . the mixture to stand in the refrigerator overnight, the orange-brown solution was poured onto crushed ice and neutralized carefully with aqueous sodhin hydroxide to a pH of 4-5. The resultant orange-red precipitate was filtered by suction and washed 10 thoroughly with water before being taken up with ether. The etherate solution as dried with anhydrous sodium sulphate, solvent was removed and the residue re-crystallized from methylene chloride to give 13 g of a yellow-brown solid. The yield of 5-mtro-l,3,3-trimethyl-indolinewas 59.6%. Example 2 5-Amino-l,3,3-trimethyl-indoline. 5-Nitro-l,3,3-trimethyl-indoline (8.16 g, 40 mmole) was added to a solution of stannous chloride (53 g, 280 mmole) in 200 ml hydrochloric acid with stirring. The mixture was gently refluxed for 16 hrs. The cold reaction mixture was pourefi onto crushed ice, made alkaline with a concentrated solution of sodium hydroxide and extracted with ethyl acetate (4 x 200 ml). The combined ethyl acetate solutions were dried with anhydrous sodium sulphate and decolorized with a small amount of active carbon. Solvent was removed and the residue re-crystallized from ethyl acetate to 5.96 g of a yellow solid, an 80 % yield of S-amino-l^^-trimethyl-indoline. Example 3a l,3,3-Trimethyl-indoline-5-yI-succinic amide Wang ester. Under nitrogen atmosphere, 5- amino-l,3,3-trimethyl indoline (1.94 g, 10.3 mmole) in 5 ml anhydrous tetrahydrofuran was added drop-wise to 5 ml THF in solution with 1.0 g (10 mmole) succinic anhydride over 1 hr. The mixture was stirred at room temperature for 7 hrs. Wang resin (theoretical loading 1.28 ramole/g, 5g, 6.4 mmole), diisopropyl carbodiimide (1.26 g, 10 mmole) and DMAP (61.1 mg, 0.5 mmole) were added. The suspension was shaken at room temperature for 24 hours and then filtered through glass sinter, washed with THF (4x15 ml), DMF (4 x 15 ml), dichloromethane (4x10 ml) and dried in vacuo. A 7.82 g bead was obtained. 11 50.9 mg of the bead was swelled in 1 ml THF and released with 0.01 M THF solution of potassium tert-butoxide (3 x 0.2 ml, 3 x 15 min.). The combined THF solution was shaken with finely powdered sodium dihydrophosphate and anhydrous sodium sulphate and filtered and washed with THF. Solvent was removed in vacuo. Chloroform was added to the residue and then removed in vacuo to remove trace THF. The residue was dried in vacuo and 1 ml, 0.01 M hexamethyldisiloxane in CDCI3 was added. From the integral of the proton of N-Me or succinyl versus that of the internal standard, the loading of l,3,3-trimethyl-indoline-5-yl-succinic amide Wang ester was calculated to be 95.6 %. Example 3b Wang resin (theoretical loading 1.28 mraole/g, 3.0 g, 3.84 mmole), 2.0 g (20 mmole) succinic acid, and 47 mg (0.38 mmole) DMAP were refluxed in THF for 48 hrs, cooled down, filtered through glass sinter and washed with THF (3x10 ml), DMF (3 x 10ml), dichloromethane (2 x 10ml), methanol ( 3 x 10 ml) and dichloromethane ( 2x10 ml). After drying in vacuo, 3.524 g of a white resin was obtained. A 1.37 g (1.5 mmole) suspension bead of the resin, 5-amino-l,3,3-trimethyl indoline (3.76 g, 2.0 mmole), HOBT (12 % water, 322 mg, 2.1 mmole) and DIC (265 mg, 2.1 mmole) in 15 ml THF were shaken overnight at room temperature under nitrogen, filtered through glass sinter and washed with THF (3x5 ml), water (2x5 ml), DMF (2x3 ml), THF (3 x 3 ml) and dichloromethane (3x3 ml). After drying in vacuo, a 1.725 g purple bead of l^,3-trimethyl-indoline-5-yl-succinic amide Wang ester was obtained. The loading was determined to be quantitative using the method as described in Example 3a. 12 Example 4 S-Succinimido-r,3f,3'-trimethyl-spiro-(2H-l-ben2opyran-2,2'-indoIine]. The bead split from Example 3a (0.243 g, corresponding to 0.2 mmole theoretical load) was swelled in 3 ml DMF under nitrogen for 1 hr, then shaken with 100 mg (0.8 mmole) salicylaldehyde at 60° C for 14 hrs then cooled, filtered through glass sinter and washed with DMF (5x3 ml) and stayed at 3 ml DMF overnight. Filtering was again carried out as well as washing with DMF (3x3 ml), dichloromethane (3x3 ml), THF (3 x 2 ml) and anhydrous THF (3x2 ml). The bead suspended in 2 ml THF was added to 0.25 ml (0.1 M) THF solution of potassium tert-butoxide, stayed for 15 to 20 minutes, filtered and washed with 1 ml anhydrous THF. The release procedure was repeated using 0.1 to 0.15 ml (0.1 M) THF solution of potassium tert-butoxide. The combined THF solution was shaken with a small amount of finely powdered sodium dihydrophosphate, filtered, solvent removed, and the residue dried in vacuo. An 80.5 mg viscous oil was obtained for a 106.6 % yield of 96.4 % purity 5-Succinimido-r,3f,3'-trimethyl-spiro-[2H-l-benzopyran-2,2f-indoline]. Example 5 6-Bromo-5'-succinimido-r,3',3'-trimethyl-spiro-[2H-l-benzopyran-2,2/-ihdoline]. The procedure of Example 4 was repeated except that 5-bromo-salicylaldehyde (121 mg, 0.6 mmole) was used. The yield of 94.3 % purity 6-bromo-S'-succinimido-r^'^'-trimethyl-spiro-pH-l-benzopyran^^'-indoIine] was 118%. Example 6 6-CMoro-5'-succimmido-l\3^3'-trimethyl-spi The procedure of Example 4 was repeated except that 5-chloro-salicylaldehyde (95 mg, 0.6 mmole) was used. The yield of 97.6 % purity 6-chloro-5'-succinimido-l%3',3'-trimethyl-spiro-[2H-l-benzopyran-2,2'-indoline]was 13 111.8%. Example 7 e-F-S'-succinimido-l'^'^'-trimethyl-spiro-t^-I-benzopyra^^Mndoline]. The procedure of Example 4 was repeated except that 5-F-salicylaldehyde (84 mg, 0.6 mmole) was used. The yield of 97.6 % purity 6-F-5'-succinimido-r,3\3>- trimethyl-spiro-[2H-I-benzopyran-2,2'-mdolinej was 119.4 %. i Example 8 6-Nitro-5'-succinimido-r,3r,3f-trimethyl-spiro-[2H-l-benzopyran-2,2'-indoIine]. The procedure of Example 4 was repeated except that 5-nitrosalicylaldehyde (100 mg, 0.6mmole) was used. The yield of 97.9 % purity 6-nitro-5f-succininiido-r^'^'-trimethyl-spiro-fZH-l-benzopyran^^'-indoline] was 119.8 %. Example 9 6,8-Dichloro-5'-succinimido-l',3',3'-trimethyl-spiro-[2H-l-ben2opyran-2^'-indoline]. The procedure of Example 4 was repeated except that 3,5- dichlorosalicylaldehyde (115 mg, 0.6 mmole) was used. The yield of 97.8 % purity 6,8-dichloro-5'-succimmido-r,3',3'-trimethyl-spiro-[2H-l-benzopyran-2,2'-indolinejwas 107.7%. Example 10 6,8-Dibromo-5'-succinimido-r,3',3'-trimethyl-spiro-[2H-l-benzopyran-232'-indoline]. The procedure of Example 4 was repeated except that 3,5- dibromosalicylaldehyde (167 mg, 0.6 mmole) was used. The yield of 97.6 % purity 6,8-diVomo-5'-succimmido-r,3',3f--trimethyl-spiro-[2H-l-benzopyran--2,2'-indolinej was 106.6 %. 14 Example 11 e^-Diiodo-S'-succinimido-r^'^'-trimethyl-spiro-tZH-l-benzopyran^^'-indoline]. The procedure of Example 4 was repeated except that 3,5-diiodosalicylaldehyde (225 mg, 0.6 mmole) was used. The yield of 97.7 % purity 6,8- Example 12 6-Bromor8-methoxy-5'-succinimido-r,3',3'-trimethyl-spiro-[2H-l-ben2opyran-2,2'-indoline]. The procedure of Example 4 was repeated except that 5-bromo-2-hydroxy-3-methoxybenzaidehyde (139 mg, 0.6 mmole) was used. The yield of 98.6 % purity 6-Bromo-8-methoxy-5f-succinimido-l',3',3'-trimethyI-spiro-[2H-l-benzopyran-2^'-indoline] was 96.1 %. Example 13 8-Fonnyl^methyl-5'-succinimido-V,3',3'-trimethyl-spiro-[2H-l-benzopyran-2,2'-indoline]. The procedure of Example 4 was repeated except that 2-hydroxy-5-methyl-1,3-benzenedicarboaJdehyde (99 mg, 0.6 mmole) was used and the suspension of bead was shaken for 24 hrs at 60° C. The yield of 96.1 % purity 8-formyl-6-methyl-5'-succinimido-r,3',3'-trimethyl-spiro-[2H-l-benzopyran-2,2'-indoline] was 98.6 %. Example 14 8-HydVoxy-5'-succinimido-r,3',3'-trimethyl-spiro-[2H-l-benzopyran-2^'-indolrae]. The procedure of Example 13 was repeated except that 2,3-dihydroxybenzaldehyde (100 mg, 0.6 mmole) was used. The yield of 8-hydroxy-5f-su(xinimido-lf^',3'-trimethyl-spiro-[2H-l-benzopyran-2^'-indoline] was 112.6 %. 15 Example 15 6-Methyl-5'-succinimido-l',3',3'-trimethyl-spiro-[2H-l-benzopyran-2,2'-indoline]. The procedure of Example 4 was repeated except that 5-methyI-salicylaldehyde (82 mg, 0.6 mmole) was used and the suspension of bead was shaken at 60° C for 24 hrs and then for 14 hrs at 80° C. The yield of 96.3 % purity 6-methyl-5'-succinimido-r,3',3'-trimethyl-spiro-[2H-l-ben2Opyran-2,2'-indoline] was 95.9 %. Example 16 S-Methyl-S'-succinimido-r^'^'-trimethyl-spiro-tiH-l-benzopyran^^'-indoUne]. The procedure of Example 15 was repeated except that 3-methyl-salicaldehyde (82 mg, 0.6mmole) was used. The yield of 96.3 % purity 6-methyl-5'-succinimido-r,3',3'-trimethyl-spiro-[2H-l-benzopyran-2,2'-indoline] was 103.7 %. Example 17 6-Memoxy-5'-succininudo-1^3^3r-lrimethyl-spu-o-[2H-]-ben2»pyran-2,2'-indoliiie]. The procedure of Example 4 was repeated except that 2-hydroxy-5-methoxybenzaldehyde (91 mg, 0.6 mmole) was used and the suspension of bead was shaken at 60° C for 18 hrs and then for 14 hrs at 80° C. The yield of 96.1 % purity 6-methoxy-5'-sucx;inimido-r,3^3'-trime1hyl-spiro-[2H-l-ben2opyran-2,2'-mdoline] was 97.2 %. Example 18 8-Ethoxy-5 '-succinimido-1 ',3 ',3 '-trimethyl-spiro-[2H-1 -benzopyran-2,2'-indoline]. The procedure of Example 17 was repeated except that 2-hydroxy-3-ethoxybenzaldehyde (99 mg, 0.6 mmole) was used. The yield of 96.4 % purity 8-ethoxy-5'-succinimido-l ',3',3'-trimethyl-spiro-[2H-l-benzopyran-2,2'-indoHne] was 98.8 %. 16 Example 19 S-Methoxy-S'-succinimido-r^'^'-trimethyl-spiro-pH-l-benzopyran^^'-indoIine]. The procedure of Example 4 was repeated except that O-vanillin (91 mg, 0.6 mmole) was used. The yield of 96.6 % purity 8-methoxy-5'-succinimido-l ',3',3f-trimethyl-spiro-[2H-l-benzopyran-2,2'-indoline] was 110 %. i Example 20 6-Trifluoromethoxy-5'-succinimido-r,3',3'-trimethyl-spiro-[2H-l-benzopyran-2,2'-indoline]. The procedure of Example 4 was repeated except that 2-hydroxy-5-trifluoromethoxybenzaldehyde (95 mg, 0.46mmole) was used and the suspension of bead was shaken at room temperature for 18 hrs and then for 3 hrs at 60° C. The yield of 6-trifluorometnoxy-5'-succinimido-l f,3',3'-trimethyl-spiro-[2H-l-benzopyran-2,2'-indoline] was 88 %. Example 21 6-Hydroxy-5^suw;inimido-l\3^3'-trimethyl-spiro-[2H-l-benzopyran-2,2'-indoline3. The procedure of Example 4 was repeated except that 2,5- dihydroxylbenzaldehyde (100 mg, 0.6 mmole) was used and the suspension of bead was shaken at 60° C for 24 hrs and then for 15 hrs at 80° C. The yield of 6-hydroxy-S'-succinimido-l^^S'-trimethyl-spiro-pH-l-benzopyran^^'-indoline] was 103.2 %. Example 22 6-tert-ButyI-5'-succinimido-1 ',3',3 '-trimethyl-spiro-[2H-1 -benzopyran-2^'-indoline]. The procedure of Example 21 was repeated except that 5-tert-butyl-salicylaldehyde (107 mg, 0.6 mmole) was used. The yield of 96.6 % purity 6-tert- 17 butyl-5'-succinimido-1 ',3 ',3'-trimethyl-spiro-[2H-1 -benzopyran-2,2'-indoline] was 89.4 %. Example 23 8-tert-ButyI-5'-succinimido-r,3',3f-trimethyl-spiro-[2H-l-ben2opyran-2^'-indoline]. The procedure of Example 21 was repeated except that 3-tert-butyl-salicyl aldehyde (107 mg, 0.6 mmole) was used and the suspension of bead was shaken at 60° C for 18 hrs and then for 14 hrs at 80° C. The yield of 96 % purity 8- tert-butyl-5'-succiiiimido-l\3',3f-triniethyI-spiro-[2H-l-ben2opyran-2^'-indolme] was 97.7 %. Example 24 6,8-Di-tert-butyl-5'-succinimido-l',3',3f-trimethyl-spiro-[2H-l-benzopyran-2^'-indoline]. The procedure of Example 4 was repeated except that 3,5-di-tert-butyl-salicyl aldehyde (180 mg, 0.6 mmole) was used and the suspension of bead was shaken at 60° C for 18 hrs and then for 14 hrs at 80° C. The yield of 95 % purity 6,8-ditert-butyl-5^succinimido-l\3\3'4rimethyl-spiro-[2H-l-benzopyran-2>2'-indoline] was 101.2 %. Example 25 7-Methoxy-5^succinimido-1^3^3'-trmiethyl-spn^-[2H-l^en2»pynm-2^Mndoline]. The procedure of Example 4 was repeated except that 2-hydroxy-4-methoxybenzaldehyde (91 mg, 0.6 mmole) was used and the suspension of bead was shaken at 60° C for 58 hrs and then for 14 hrs at 80° C. The yield of 7-methoxy-5'-succmimido-l\3\3^trimethyl-spiro-[2H-l-benzopyran-2^MndoHne] was 82.1 %. 18 Example 26 5,7-Dimethoxy-5f-succinimido-l',3',3'-trimethyI-spiro-[2H-l-benzopyran-2,2'-indoline]. The procedure of Example 4 was repeated except that 2-hydroxy-4,6-dimethoxybenzaldehyde (240 mg, 2.0 mmole) was used and the suspension of bead was shaken at 60° C for 24 hrs and then for 11 days at 80° C. The yield of 96.8 % purity 5,7-dimethoxy-5r-succinimido-]\3\3'-trimethyl-spiro-[2H-l-benzopyraii-2,2'-indoline] was 82.9 %. Example 27 8-Methoxy-6-nitro-5f-sucdmmido-r>3',3r-trimethyl-spiro-[2H-l-benzopyran-2,2'-indoline]. 162 mg of the bead split from Example 3b and corresponding to 0.14 mmole theoretical loading was swelled in 3ml DMF under nitrogen for 1 hr, shaken with 8-methoxy-5-nitro salicyl aldehyde (83 mg, 0.42 mmole) at 60° C for 14 hrs. The suspension was cooled, filtered through glass sinter, washed with DMF (5x1 ml), stayed at 3 ml DMF overnight, filtered again and washed with DMF (3 x 1ml), THF (3x1 ml), dichloromethane (3 x 2ml) and THF (3x2 ml). To the bead suspended in 1 ml THF was added 1 ml, 0.1 M THF solution of DBU, stayed for 10 hrs, filtered and washed with THF (2x1 ml). The release procedure was repeated twice. The combined THF solution was passed by a short silica column eluted with THF to remove DBU. Solvent was removed and the residue dried in vacuo for a 104.6 % yield of 8-methoxy^-nit^o-5'-succmimido-1^3^3f-t^imethyl-spi^o-[2H-l-benzopyran-2,2'-indoline]. 19 We Claim: 1. A compound comprising the formula I: wherein R1 is a C1-C4 alkyl, phenyl, benzyl, ally!, or ethoxycarbonyl ethyl, R2 and R3 are each independently meth'yl, ethyl, or phenyl, R4 is hydrogen, hydroxy, trichioromethyl, trifluoromethyl, formyl, C1-C4 alkyl, halogen, C1-C4 alkoxy, nitro, cyano, Ci-C4 monohaloalkyl, C1-C4 alkoxycarbonyl, or an aromatic sharing the two adjacent carbon atoms with the benzene portion of the pyran ring to form a condensed aromatic ring, and x equals 1, 2, or 3. 2. The compound as claimed in claim 1, wherein when x = 1, R4 is located on any of the available carbon atoms of the benzene ring of the benzopyran moiety. 3. The compound as claimed in claim 2, wherein R4 is located on the 6, 7 or 8 position of the benzene ring of the benzopyran moiety. 20 4. The compound as claimed in claim 1, wherein when x = 2, each R4 is the same or different and located at the 6 and 8 or 5 and 7 positions of the benzene ring of the benzopyran moiety. 5. The compound as claimed in claim 3, wherein each R4 is located at the 6 and 8 positions of the benzene ring of the benzopyran moiety. 6. The compound as claimed in claim 1 comprising the formula: wherein R4 is hydrogen, hydroxy, trifluoromethyl, formyl, methyl, ethyl, methoxy, ethoxy, nitro, fluoro, chloro, bromo, or iodo, and x is 1 or 2. 7, An article comprising a compound of formula I: 21 wherein R1 is a C1-C4 alkyl, phenyl, benzyl, atlyl, or ethoxycarbonyl ethyl, R2 and R3 are each independently methyl, ethyl, or phenyl and R4 is C1-C4 alkyl, C1-C2 alkoxy, chloro, bromo, iodo, trifluoromethyl, or nitro, R4 is hydrogen, hydroxy, trichtoromethyl, trifluoromethyl, formyl, C1-C4 alkyl, halogen, C1-C4 alkoxy, nitro, cyano, C1-C1 monohaloalkyl, C1-C4 alkoxycarbonyl, or an aromatic sharing the two adjacent carbon atoms with the benzene portion of the pyran ring to form a condensed aromatic ring, and x equals 1, 2, or 3. 8." The article as claimed in claim 6, wherein in the compound when x = 1, R4 is located on any of the available carbon atoms of the benzene ring of the benzopyran moiety. 22 9. The article as claimed in claim 8, wherein in the compound R4 is located on the 6, 7, or 8 position of the benzene ring of the benzopyran moiety. 10. The article as claimed in claim 6, wherein in the compound when x = 2, each R4 be the same or different and located at the 6 and 8 or 5 and 7 positions of the benzene ring of the benzopyran moiety. 11. The article as claimed in claim 10, wherein each R4 is located at the 6 and 8 positions the benzene ring of the benzopyran moiety. 12.The article as claimed in claim 6, wherein the article is an ophthalmic lens. 1 3. The article as claimed in claim 7 comprising a compound of the formula II: 23 wherein R4 is hydrogen, hydroxy, trifluoromethyl, formyl, methyl, ethyl, methoxy, ethqxy, nitro, fluoro, chloro, bromo, or iodo, and x is 1 or 2. 14. The article as claimed in claim 13, wherein the article is an ophthalmic lens. 15. A method for producing an indolinospiropyran compound as claimed in claim 1 comprising the steps of: (i) providing an indoline loaded resin of the formula: wherein R1 is a C1-C4 alkyl, phenyl, benzyl, allyl, or ethoxycarbonyl ethyl, R2 and R3 are each independently methyl, ethyl, or phenyl and R4 is C1-C4 alkyl, C1-C2 alkoxy, chloro, bromo, iodo, trifluoromethyl, or nitro; 24 (ii) treating the loaded resin at a temperature of 50°C to 120°C under an inert atmosphere for a time of 14 hours to 11 days, with 1.1. to 10 equivalents of a salicylaldehyde derivative of the formula VI: wherein R4 is hydrogen, hydroxy, trichloromethyl, formyl, C1-C4 atkyl, halogen, C1-C4 alkoxy, nitro, cyano, C1-C1 monohaloalkyl, C1-C4 alkoxycarbonyl, or an aromatic sharing the two adjacent carbon atoms with the benzene portion of the pyran ring to form a condensed aromatic ring, and x equals 1, 2, or 3 to produce the indolinospiropyran compound; and (iii) releasing the indolinospiropyran compound. 16. The method as claimed in claim 15 wherein the resin is a hydroxy resin. 25 1 7. The method as claimed in claim 16, wherein the hydroxy resin is a hydroxymethyl polystyrene resin, a Wang resin, HMPA-PEGA resin, HMPB-BHA resin, HMPB-MBHA resin, or a combination thereof. 18. The method as claimed in claim 15, wherein the resin is of an 100-200 mesh or an 200-400 mesh. W-rThe method as claimed in claim 17, wherein an 100-200 mesh, high loading hydroxymethyl polystyrene resin or a Wang resin is used. 20. The method as claimed in claim 1 5, further comprising the step of preparing the indoiine loaded resin by: (i) treating an aminoindoline compound of the formula wherein R1 is a C1-C4 alky I, phenyl, benzyl, allyl, or ethoxycarbonyl ethyl, R2 and R3 are each independently methyl, 26 ethyl, or phenyl and R4 is C1-C4 alkyl, C1-C2 alkoxy, chloro, bromo, iodo, trifluoromethyl, or nitro, with succinic acid at a temperature of 0° to 60°C under inert atmosphere for 3 to 24 hours. (ii) adding, subsequently to the mixture a hydroxy resin, diisopropyl carbodiimide and dimethylamino pyridine to form a suspension bead; and (iii) shaking the suspension bead at a temperature of 0°C to 60°C for 14 to 24 hours under inert atmosphere to form the indoline loaded resin. 21. The method as claimed in claim 15, comprising preparing the indoline loaded resin by: (i) shaking an excess of hydroxy resin with an excess of succinic anhydride at a temperature of 60 to 120°C for 24 to 48 hours to couple the resin and succinic acid; (ii) washing away, subsequently, excess succinic anhydride; and 27 (Mi) treating, with 1-hydroxy benzotriazole and DIC at 0 to 60°C and under an inert atmosphere, the coupled resin and succinic acid with an aminoindoline of the formula IV: wherein R1 is a C1-C4 alkyl, phenyl, benzyl, allyl, or ethoxycarbonyl ethyl, R2 and R3 are each independently methyl, ethyl, or phenyl and R4 is C1-C4 alkyl, C1-C2 alkoxy, chloro, bromo, iodo, trifluoromethyl, or nitro. 22. The method as claimed in claim 20, comprising the steps of forming the aminoindoline compound by: reducing a nitroindoline derivative of the formula V: wherein R1 is a C1-C4 alkyl, phenyl, benzyl, allyl, or ethoxycarbonyl ethyl, R2 and R3 are each independently methyl, ethyl, or phenyl and R4 is C1-C2 alkoxy, chioro, bromo, iodo, trifluoromethyl, or nitro. 28 23. The method as claimed in claim 21, comprising the steps of forming the aminoindoline compound by: reducing a nitroindoline derivative of the formula: wherein R1 is a C1-C4 alkyl, phenyl, benzyl, allyl, or ethoxycarbonyl ethyl, R2 and R3 are each independently methyl, ethyl, or phenyl and R4 is C1-C2 alkoxy, chloro, bromo, iodo, trifluoromethyl, or nitro. 24. An indoline loaded resin usable in the method as claimed in claim 1 5 of the formula: 29 wherein R1 is a C1-C4 alkyl, phenyl, benzyl, allyl, or ethoxycarbonyl ethyl, R2 and R3 are each independently methyl, ethyl, or phenyl and R4 is C1-C2 alkoxy, chloro, bromo, iodo, trifluoromethyl, or nitro. A compound comprising the formula: wherein R1 is a C1-C4 alkyl, phenyl, benzyl, allyl, or ethoxycarbonyl ethyl, R2 and R3 are each independently methyl, ethyl, or phenyl, R4 is hydrogen, hydroxy, trichloromethyl, trifluoromethyl, formyl, C1-C4 alkyl, halogen, C1-C4 alkoxy, nitro, cyano, C1-C4 monohaloalkyl, C1-C4 alkoxycarbonyl, or an aromatic sharing the two adjacent carbon atoms with the benzene portion of the pyran ring to form a condensed aromatic ring, and x equals 1, 2, or 3.

Full Text

1
INDOLINOSPIROPYRAN COMPOUNDS AND METHODS FOR THEIR
MANUFACTURE
Field of the Invention
The present invention relates to spiropyran,compounds. In particular, the invention provides indolinospiropyran compounds and methods for their manufacture, which compounds are useful as photochromic compounds.
Background of the Invention
Various classes of photochromic compounds have been synthesized, and suggested for use in applications in which reversible color changes or darkening is induced by sunlight. For example, spiro(indolino)naphthopyrans and spiro(indolino)quinopyrans are described in GB Patent 2,174,711. Spiropyrans also are described in Brown, Glenn H. ed., Photochromism (New York, 1971) and Durr, Heinz and Henri Bouas-Laurent eds., Photochromism (Elsevier, 1990).
Spiropyran derivatives may be the best known organic compounds showing photochromism phenomenon, but the structures of reported spiropyrans are considerably limited. Thus, a need exists both for spiropyran compounds allowing further facile modifications as well as methods for the synthesis of diverse spiropyran compounds.
Description of the Invention and its Preferred Embodiments The present invention provides indolinospiropyran compounds, and particularly photochromic indolinospiropyran compounds, as well as methods for synthesizing these compounds. The indolinospiropyran compounds of the invention are substituted on the indole ring with succinimide, which substitution permits ring opening of the succinimide and modulation of the bulk and photochromic properties of the compounds.

2
In one embodiment, the invention provides a compound comprising, consisting essentially of, and consisting of the formula:

wherein R1 is C1- C18 alkyl, allyl, phenyl, mono- or disubstituted phenyl, phen(C1 -C4 )alkyl, or (C1 -C4)alkoxycarDonyl (C1-C4 )alkyl, R2and R3 are each independently C1 -C4alkyl, phenyl, mono- or di-substituted phenyl, benzyl, or combined to form a cyclic ring that is cyclohexyl, norbomyl or adamantyl ring, K4 is hydrogen, hydroxy, trichloromethyl, trifluoromethyl, formyl, C1-C4 alkyl, halogen, C1- C4 alkoxy, nitro, cyano, C1-C4 monohaloalkyl, C1-C4 alkoxycarbonyl, or an aromatic sharing the two adjacent carbon atoms with the benzene portion of the pyran ring to form a condensed aromatic ring including, without limitation, naphthyl, phenanthrenyl, and quinolino, x equals 1,2, or 3 provided that when x = V, R4 may be located on any of the available carbon atoms of the benzene ring of the benzopyran moiety, preferably on the 6,7, or 8 position and when x = 2, each of the R4 may be the same or different and located at the 6 and 8 or 5 and 7 positions, preferably at the 6 and 8 positions. R1 preferably is a C1 -C4 alkyl, phenyl, benzyl allyl, or ethoxycarbonyl ethyl, R2 and R3 preferably are each independently methyl, ethyl, or phenyl and R4 preferably is C1-C4 alkyl, C1-C2 alkoxy, chloro, bromo, iodo, trifluoromethyl, or nitro.
In a preferred embodiment, the invention provides a compound comprising, consisting essentially of, and consisting of the formula:

3

wherein R4 is hydrogen, hydroxy, trifluoromethyl, formyl, methyl, ethyl, methoxy, ethoxy, nitro, fluoro, chloro, bromo, or iodo, and x is 1 or 2.
Because the compounds of formulae I and II contain a succinimide portion, the properties of the compounds, such as solubility, sensitivity and the like, of the invention may be manipulated by ring opening of the succinimide using any of a variety of known methods. Suitable such methods are described, for example, in
48(12) Heterocycles, 2677-2691 (1998).

Formulae I and II compounds may be prepared by any convenient known method and preferably are prepared using a solid phase organic synthesis. The use of solid phase synthesis is advantageous in that it provides ease in execution of the reaction, ease in product purification, and convenient handling,of polar molecules throughout the synthetic protocol. Additionally, this approach permits use of commercially available starting materials and use of excess reactant to drive the reaction to completion and to surpass the side reactions. Key in the synthesis is the use of a polymer-supported indoline of the formula:

4
wherein R1, R2, and R3 are the same as for fonnula (I). The solid support may be selected from any of a variety of hydroxy resins. Suitable hydroxy resins include, without limitation, bydroxyroethyl polystyrene resin, Wang resin (also known as 4-hvdroxymethyl phenpxy resin or "HMP resin"), HMPA-PEGA resin (or 4-hydroxymethylphenoxyacetic acid and bisacrylamidoprop-l-yl polyethyleneglycol), HMPB-BHA resin (or4-hydroxy-3-methoxyphenoxybutyric acid benzhydrylamine), HMPB-MBHA resin (4-hydroxymethyl~3-methoxyphenoxybutyric acid-methylbenzhydrylamine), and combinations thereof. The theoretical loading of the resin may be either low (e.g.7 less than about 0.1 mmole/g) or high (e.g., greater than about 0.4 mmole/g), but for production of greater quantities of product, preferably is high, more preferably about 0.4 to about 1.5 mmole/g. Either of about 100-200 mesh or about 200-400 mesh resin may be used. Preferred resins are an about 100-200 mesh high loading hydroxymethyl polystyrene or a Wang resin.
The solid support used will depend upon the reactants selected, the solvent used, and the product desired. The. resin preferably has types and quantities of functional groups that permit, efficient attachment of the reactants as well as efficient release of the product Additionally, the resin must be swellable in the solvent-used. The amount of resin used will depend on the amount of reactants used and the reaction scale desired. Generally, about 1 mg to about 100 g of resin may be used.
Fonnula m compounds, may be prepared by either of two reaction schemes using.an aminoindoline compound of the following formula:

5

wherein R1, R3, and R3 are as defined for formula I.
The reaction schemes for the preparation of the formula in compound using the aminoindolino compounds are as follows:

6
In Metthod A, an aminoindoline compound of formula IV, is treated with succinic anhydride under conditions suitable to form succinamic acid. More specifically, the reaction is carried out at a temperature of about 0 to about 60° C, preferably about room temperature, under an inert atmosphere including, without limitation, argon or nitrogen, for about 3 to 24 hours. The amount of reactants used will depend on the amount of product desired and typically will be about 1 mg to about 100 g, preferabry about 100 mg to about 10 g. A hydroxy resin along with diisopropyl carbodiimide ("DIC") and dimethylamino pyridine ("DMAP") then are added to the mixture to form a suspension bead. This suspension of bead is shaken under conditions suitable to carry out a coupling reaction. Suitable conditions for the reaction are a temperature of about 0 to about 60° C, preferably about room temperature, about 14 to 24 hours under an inert atmosphere. Progress of the coupling reaction may be monitored by any convenient means including, without limitation, FT-IR or single bead FT-IR. Typically, the reaction forming the mdoline loaded resin is complete after 24 hours at room temperature.
In Method B, hydroxy resin is shaken with an excess of succinic anhydride under conditions suitable to carry out a coupling reaction. Suitable conditions for the reaction are a temperature of about 60 to 120° C, preferably about 70 to about 100° C, for about 10 to about 60 hours, preferably about 24 to about 48 hours. The progress of the reaction may be monitored by any convenient means and, generally, will be complete after 48 hours of refluxing. After completion of the reaction, excess succinic anhydride is washed away and the resin, now coupled with the succinic acid, is treated with an aminoindoline of formula IV along withal-hydroxy benzotriazole ("HOBT") and DIC. Conditions suitable for the treatment are a temperature of about 0 to 60° C, preferably room temperature, and an inert atmosphere.
In either method, loading typically is over about 95 percent Any convenient method for testing loading may be used including, without limitation, as disclosed in

7
63(3) J. Org. Chem., 708 -718 (1998). Hexamethyidisiloxane ("HMDSO") may be used as an internal standard for purposes of 1HNMR testing.
The aminoindoline compounds of formula IV_may be prepared by reduction of nitroindoline derivatives of the formula;

wherein Rl, R2, and R3 are as for formula I. Suitable nitroindoline derivatives for formula V may be prepared by nitration of indoline derivatives as described in 101(8) Bull Soc. Chim.Bdg., 719-739 (1992). The indoline derivatives may be nitrated by nitric acid in cold sulfuric acid below 10° C, preferably below 7° C, over about 10 hours.
In solid phase synthesis of the indolinospiropyran compounds of the invention, a resin loaded with indoline, generally about 100 mg to about 100 g, preferably about 100 mg to about 10 g, as in formula ID may be split and treated with any of a variety of salicylaldehyde derivatives under conditions suitable to form the desired indolinospiropyran compound. Suitable conditions for carrying out the synthesis are a temperature of about 50 to 120° C under an inert atmosphere for a time of about 14 to about 11 days, preferably about 14 hours to about 3 days. Suitable salicylaldehyde derivatives are of the formula:

8

wherein R4 and x are as for formula I.
The solid phase synthesis proceeds as follows:

The synthesis is carried out in any suitable solvent, preferably in dioxane, dimethylformamide ("DMF"), N-methylprroiidone ("NMP"), tetrahydrofiiran ("THF"), or combinations thereof. Release of the formula I compound from the solid support may be carried out in any convenient manner such as by a base-catalyzed process using any suitable base including, without limitation, piperidine, DBU, sodium methoxide, potassium tert-butyloxide. In general, about 3 to about 4

9
equivalents of salicylaidehyde derivative is used to react with about 1.1 to about 10 equivalents, preferably about 2 to about 5, more preferably about 3 to about 4 equivalents of resin loaded with indoline.
The compounds of the invention are useful in any of the wide variety of applications, such as ophthalmic lenses, windshields, windows, and the like in which photochromism is useful. More specifically, an effective amount of the compounds of the invention are incorporated into or coate(Lpnto_anjophthaimic lens. windshield, window, or like article. The article into which the compound is incorporated or onto which it is coated will darken with exposure to ultra-violet light and revert to its original color or colorless condition in when not exposed to UV light at ambient temperatures. An effective amount of the compound is an amount of about 10-5 to aboutl 10-2 mol/preferably about 10""5 to about 10"3 mol/1. Methods for incorporating into or coating such articles with photochromic compounds such as those of the invention are well know in the art
The invention will be clarified by considering the following, non-limiting examples.
Examples Example 1 5-Nitro-1,3,3-trimethyl-indoline.
2,3,4-Trimethyl-indoline (17.3, 0.1 mole) was added to 45 ml cold sulfuric acid drop-wise at below 5° C with stirring. 7.0 g Nitric acid (1 mole) in 18 mL sulfuric acid then were added drop-wise with stirring over 1 hr while keeping the. temperature below 7° C. Stirring was continued for 3 hrs at 7° C. After allowing . the mixture to stand in the refrigerator overnight, the orange-brown solution was poured onto crushed ice and neutralized carefully with aqueous sodhin hydroxide to a pH of 4-5. The resultant orange-red precipitate was filtered by suction and washed

10
thoroughly with water before being taken up with ether. The etherate solution as dried with anhydrous sodium sulphate, solvent was removed and the residue re-crystallized from methylene chloride to give 13 g of a yellow-brown solid. The yield of 5-mtro-l,3,3-trimethyl-indolinewas 59.6%.
Example 2 5-Amino-l,3,3-trimethyl-indoline.
5-Nitro-l,3,3-trimethyl-indoline (8.16 g, 40 mmole) was added to a solution of stannous chloride (53 g, 280 mmole) in 200 ml hydrochloric acid with stirring. The mixture was gently refluxed for 16 hrs. The cold reaction mixture was pourefi onto crushed ice, made alkaline with a concentrated solution of sodium hydroxide and extracted with ethyl acetate (4 x 200 ml). The combined ethyl acetate solutions were dried with anhydrous sodium sulphate and decolorized with a small amount of active carbon. Solvent was removed and the residue re-crystallized from ethyl acetate to 5.96 g of a yellow solid, an 80 % yield of S-amino-l^^-trimethyl-indoline.
Example 3a l,3,3-Trimethyl-indoline-5-yI-succinic amide Wang ester.
Under nitrogen atmosphere, 5- amino-l,3,3-trimethyl indoline (1.94 g, 10.3 mmole) in 5 ml anhydrous tetrahydrofuran was added drop-wise to 5 ml THF in solution with 1.0 g (10 mmole) succinic anhydride over 1 hr. The mixture was stirred at room temperature for 7 hrs. Wang resin (theoretical loading 1.28 ramole/g, 5g, 6.4 mmole), diisopropyl carbodiimide (1.26 g, 10 mmole) and DMAP (61.1 mg, 0.5 mmole) were added. The suspension was shaken at room temperature for 24 hours and then filtered through glass sinter, washed with THF (4x15 ml), DMF (4 x 15 ml), dichloromethane (4x10 ml) and dried in vacuo. A 7.82 g bead was obtained.

11
50.9 mg of the bead was swelled in 1 ml THF and released with 0.01 M THF solution of potassium tert-butoxide (3 x 0.2 ml, 3 x 15 min.). The combined THF solution was shaken with finely powdered sodium dihydrophosphate and anhydrous sodium sulphate and filtered and washed with THF. Solvent was removed in vacuo. Chloroform was added to the residue and then removed in vacuo to remove trace THF. The residue was dried in vacuo and 1 ml, 0.01 M hexamethyldisiloxane in CDCI3 was added. From the integral of the proton of N-Me or succinyl versus that of the internal standard, the loading of l,3,3-trimethyl-indoline-5-yl-succinic amide Wang ester was calculated to be 95.6 %.
Example 3b
Wang resin (theoretical loading 1.28 mraole/g, 3.0 g, 3.84 mmole), 2.0 g (20 mmole) succinic acid, and 47 mg (0.38 mmole) DMAP were refluxed in THF for 48 hrs, cooled down, filtered through glass sinter and washed with THF (3x10 ml), DMF (3 x 10ml), dichloromethane (2 x 10ml), methanol ( 3 x 10 ml) and dichloromethane ( 2x10 ml). After drying in vacuo, 3.524 g of a white resin was obtained.
A 1.37 g (1.5 mmole) suspension bead of the resin, 5-amino-l,3,3-trimethyl indoline (3.76 g, 2.0 mmole), HOBT (12 % water, 322 mg, 2.1 mmole) and DIC (265 mg, 2.1 mmole) in 15 ml THF were shaken overnight at room temperature under nitrogen, filtered through glass sinter and washed with THF (3x5 ml), water (2x5 ml), DMF (2x3 ml), THF (3 x 3 ml) and dichloromethane (3x3 ml). After drying in vacuo, a 1.725 g purple bead of l^,3-trimethyl-indoline-5-yl-succinic amide Wang ester was obtained. The loading was determined to be quantitative using the method as described in Example 3a.

12
Example 4 S-Succinimido-r,3f,3'-trimethyl-spiro-(2H-l-ben2opyran-2,2'-indoIine].
The bead split from Example 3a (0.243 g, corresponding to 0.2 mmole theoretical load) was swelled in 3 ml DMF under nitrogen for 1 hr, then shaken with 100 mg (0.8 mmole) salicylaldehyde at 60° C for 14 hrs then cooled, filtered through glass sinter and washed with DMF (5x3 ml) and stayed at 3 ml DMF overnight. Filtering was again carried out as well as washing with DMF (3x3 ml), dichloromethane (3x3 ml), THF (3 x 2 ml) and anhydrous THF (3x2 ml).
The bead suspended in 2 ml THF was added to 0.25 ml (0.1 M) THF solution of potassium tert-butoxide, stayed for 15 to 20 minutes, filtered and washed with 1 ml anhydrous THF. The release procedure was repeated using 0.1 to 0.15 ml (0.1 M) THF solution of potassium tert-butoxide. The combined THF solution was shaken with a small amount of finely powdered sodium dihydrophosphate, filtered, solvent removed, and the residue dried in vacuo. An 80.5 mg viscous oil was obtained for a 106.6 % yield of 96.4 % purity 5-Succinimido-r,3f,3'-trimethyl-spiro-[2H-l-benzopyran-2,2f-indoline].
Example 5 6-Bromo-5'-succinimido-r,3',3'-trimethyl-spiro-[2H-l-benzopyran-2,2/-ihdoline].
The procedure of Example 4 was repeated except that 5-bromo-salicylaldehyde (121 mg, 0.6 mmole) was used. The yield of 94.3 % purity 6-bromo-S'-succinimido-r^'^'-trimethyl-spiro-pH-l-benzopyran^^'-indoIine] was 118%.
Example 6 6-CMoro-5'-succimmido-l\3^3'-trimethyl-spi
The procedure of Example 4 was repeated except that 5-chloro-salicylaldehyde (95 mg, 0.6 mmole) was used. The yield of 97.6 % purity 6-chloro-5'-succinimido-l%3',3'-trimethyl-spiro-[2H-l-benzopyran-2,2'-indoline]was

13
111.8%.
Example 7
e-F-S'-succinimido-l'^'^'-trimethyl-spiro-t^-I-benzopyra^^Mndoline]. The procedure of Example 4 was repeated except that 5-F-salicylaldehyde (84 mg, 0.6 mmole) was used. The yield of 97.6 % purity 6-F-5'-succinimido-r,3\3>-
trimethyl-spiro-[2H-I-benzopyran-2,2'-mdolinej was 119.4 %.
i
Example 8 6-Nitro-5'-succinimido-r,3r,3f-trimethyl-spiro-[2H-l-benzopyran-2,2'-indoIine].
The procedure of Example 4 was repeated except that 5-nitrosalicylaldehyde (100 mg, 0.6mmole) was used. The yield of 97.9 % purity 6-nitro-5f-succininiido-r^'^'-trimethyl-spiro-fZH-l-benzopyran^^'-indoline] was 119.8 %.
Example 9
6,8-Dichloro-5'-succinimido-l',3',3'-trimethyl-spiro-[2H-l-ben2opyran-2^'-indoline].
The procedure of Example 4 was repeated except that 3,5-
dichlorosalicylaldehyde (115 mg, 0.6 mmole) was used. The yield of 97.8 % purity 6,8-dichloro-5'-succimmido-r,3',3'-trimethyl-spiro-[2H-l-benzopyran-2,2'-indolinejwas 107.7%.
Example 10
6,8-Dibromo-5'-succinimido-r,3',3'-trimethyl-spiro-[2H-l-benzopyran-232'-indoline].
The procedure of Example 4 was repeated except that 3,5-
dibromosalicylaldehyde (167 mg, 0.6 mmole) was used. The yield of 97.6 % purity 6,8-diVomo-5'-succimmido-r,3',3f--trimethyl-spiro-[2H-l-benzopyran--2,2'-indolinej was 106.6 %.

14
Example 11
e^-Diiodo-S'-succinimido-r^'^'-trimethyl-spiro-tZH-l-benzopyran^^'-indoline].
The procedure of Example 4 was repeated except that 3,5-diiodosalicylaldehyde (225 mg, 0.6 mmole) was used. The yield of 97.7 % purity 6,8- Example 12
6-Bromor8-methoxy-5'-succinimido-r,3',3'-trimethyl-spiro-[2H-l-ben2opyran-2,2'-indoline].
The procedure of Example 4 was repeated except that 5-bromo-2-hydroxy-3-methoxybenzaidehyde (139 mg, 0.6 mmole) was used. The yield of 98.6 % purity 6-Bromo-8-methoxy-5f-succinimido-l',3',3'-trimethyI-spiro-[2H-l-benzopyran-2^'-indoline] was 96.1 %.
Example 13
8-Fonnyl^methyl-5'-succinimido-V,3',3'-trimethyl-spiro-[2H-l-benzopyran-2,2'-indoline].
The procedure of Example 4 was repeated except that 2-hydroxy-5-methyl-1,3-benzenedicarboaJdehyde (99 mg, 0.6 mmole) was used and the suspension of bead was shaken for 24 hrs at 60° C. The yield of 96.1 % purity 8-formyl-6-methyl-5'-succinimido-r,3',3'-trimethyl-spiro-[2H-l-benzopyran-2,2'-indoline] was 98.6 %.
Example 14 8-HydVoxy-5'-succinimido-r,3',3'-trimethyl-spiro-[2H-l-benzopyran-2^'-indolrae].
The procedure of Example 13 was repeated except that 2,3-dihydroxybenzaldehyde (100 mg, 0.6 mmole) was used. The yield of 8-hydroxy-5f-su(xinimido-lf^',3'-trimethyl-spiro-[2H-l-benzopyran-2^'-indoline] was 112.6 %.

15
Example 15
6-Methyl-5'-succinimido-l',3',3'-trimethyl-spiro-[2H-l-benzopyran-2,2'-indoline].
The procedure of Example 4 was repeated except that 5-methyI-salicylaldehyde (82 mg, 0.6 mmole) was used and the suspension of bead was shaken at 60° C for 24 hrs and then for 14 hrs at 80° C. The yield of 96.3 % purity
6-methyl-5'-succinimido-r,3',3'-trimethyl-spiro-[2H-l-ben2Opyran-2,2'-indoline] was 95.9 %.
Example 16 S-Methyl-S'-succinimido-r^'^'-trimethyl-spiro-tiH-l-benzopyran^^'-indoUne].
The procedure of Example 15 was repeated except that 3-methyl-salicaldehyde (82 mg, 0.6mmole) was used. The yield of 96.3 % purity 6-methyl-5'-succinimido-r,3',3'-trimethyl-spiro-[2H-l-benzopyran-2,2'-indoline] was 103.7 %.
Example 17 6-Memoxy-5'-succininudo-1^3^3r-lrimethyl-spu-o-[2H-]-ben2»pyran-2,2'-indoliiie].
The procedure of Example 4 was repeated except that 2-hydroxy-5-methoxybenzaldehyde (91 mg, 0.6 mmole) was used and the suspension of bead was shaken at 60° C for 18 hrs and then for 14 hrs at 80° C. The yield of 96.1 % purity 6-methoxy-5'-sucx;inimido-r,3^3'-trime1hyl-spiro-[2H-l-ben2opyran-2,2'-mdoline] was 97.2 %.
Example 18 8-Ethoxy-5 '-succinimido-1 ',3 ',3 '-trimethyl-spiro-[2H-1 -benzopyran-2,2'-indoline].
The procedure of Example 17 was repeated except that 2-hydroxy-3-ethoxybenzaldehyde (99 mg, 0.6 mmole) was used. The yield of 96.4 % purity 8-ethoxy-5'-succinimido-l ',3',3'-trimethyl-spiro-[2H-l-benzopyran-2,2'-indoHne] was 98.8 %.

16
Example 19
S-Methoxy-S'-succinimido-r^'^'-trimethyl-spiro-pH-l-benzopyran^^'-indoIine]. The procedure of Example 4 was repeated except that O-vanillin (91 mg, 0.6 mmole) was used. The yield of 96.6 % purity 8-methoxy-5'-succinimido-l ',3',3f-trimethyl-spiro-[2H-l-benzopyran-2,2'-indoline] was 110 %.
i Example 20
6-Trifluoromethoxy-5'-succinimido-r,3',3'-trimethyl-spiro-[2H-l-benzopyran-2,2'-indoline].
The procedure of Example 4 was repeated except that 2-hydroxy-5-trifluoromethoxybenzaldehyde (95 mg, 0.46mmole) was used and the suspension of bead was shaken at room temperature for 18 hrs and then for 3 hrs at 60° C. The yield of 6-trifluorometnoxy-5'-succinimido-l f,3',3'-trimethyl-spiro-[2H-l-benzopyran-2,2'-indoline] was 88 %.
Example 21 6-Hydroxy-5^suw;inimido-l\3^3'-trimethyl-spiro-[2H-l-benzopyran-2,2'-indoline3.
The procedure of Example 4 was repeated except that 2,5-
dihydroxylbenzaldehyde (100 mg, 0.6 mmole) was used and the suspension of bead was shaken at 60° C for 24 hrs and then for 15 hrs at 80° C. The yield of 6-hydroxy-S'-succinimido-l^^S'-trimethyl-spiro-pH-l-benzopyran^^'-indoline] was 103.2 %.
Example 22
6-tert-ButyI-5'-succinimido-1 ',3',3 '-trimethyl-spiro-[2H-1 -benzopyran-2^'-indoline].
The procedure of Example 21 was repeated except that 5-tert-butyl-salicylaldehyde (107 mg, 0.6 mmole) was used. The yield of 96.6 % purity 6-tert-

17
butyl-5'-succinimido-1 ',3 ',3'-trimethyl-spiro-[2H-1 -benzopyran-2,2'-indoline] was 89.4 %.
Example 23
8-tert-ButyI-5'-succinimido-r,3',3f-trimethyl-spiro-[2H-l-ben2opyran-2^'-indoline].
The procedure of Example 21 was repeated except that 3-tert-butyl-salicyl aldehyde (107 mg, 0.6 mmole) was used and the suspension of bead was shaken at 60° C for 18 hrs and then for 14 hrs at 80° C. The yield of 96 % purity 8-
tert-butyl-5'-succiiiimido-l\3',3f-triniethyI-spiro-[2H-l-ben2opyran-2^'-indolme] was 97.7 %.
Example 24
6,8-Di-tert-butyl-5'-succinimido-l',3',3f-trimethyl-spiro-[2H-l-benzopyran-2^'-indoline].
The procedure of Example 4 was repeated except that 3,5-di-tert-butyl-salicyl aldehyde (180 mg, 0.6 mmole) was used and the suspension of bead was shaken at 60° C for 18 hrs and then for 14 hrs at 80° C. The yield of 95 % purity 6,8-ditert-butyl-5^succinimido-l\3\3'4rimethyl-spiro-[2H-l-benzopyran-2>2'-indoline] was 101.2 %.
Example 25 7-Methoxy-5^succinimido-1^3^3'-trmiethyl-spn^-[2H-l^en2»pynm-2^Mndoline].
The procedure of Example 4 was repeated except that 2-hydroxy-4-methoxybenzaldehyde (91 mg, 0.6 mmole) was used and the suspension of bead was shaken at 60° C for 58 hrs and then for 14 hrs at 80° C. The yield of 7-methoxy-5'-succmimido-l\3\3^trimethyl-spiro-[2H-l-benzopyran-2^MndoHne] was 82.1 %.

18
Example 26
5,7-Dimethoxy-5f-succinimido-l',3',3'-trimethyI-spiro-[2H-l-benzopyran-2,2'-indoline].
The procedure of Example 4 was repeated except that 2-hydroxy-4,6-dimethoxybenzaldehyde (240 mg, 2.0 mmole) was used and the suspension of bead was shaken at 60° C for 24 hrs and then for 11 days at 80° C. The yield of 96.8 % purity 5,7-dimethoxy-5r-succinimido-]\3\3'-trimethyl-spiro-[2H-l-benzopyraii-2,2'-indoline] was 82.9 %.
Example 27
8-Methoxy-6-nitro-5f-sucdmmido-r>3',3r-trimethyl-spiro-[2H-l-benzopyran-2,2'-indoline].
162 mg of the bead split from Example 3b and corresponding to 0.14 mmole theoretical loading was swelled in 3ml DMF under nitrogen for 1 hr, shaken with 8-methoxy-5-nitro salicyl aldehyde (83 mg, 0.42 mmole) at 60° C for 14 hrs. The suspension was cooled, filtered through glass sinter, washed with DMF (5x1 ml), stayed at 3 ml DMF overnight, filtered again and washed with DMF (3 x 1ml), THF (3x1 ml), dichloromethane (3 x 2ml) and THF (3x2 ml). To the bead suspended in 1 ml THF was added 1 ml, 0.1 M THF solution of DBU, stayed for 10 hrs, filtered and washed with THF (2x1 ml). The release procedure was repeated twice. The combined THF solution was passed by a short silica column eluted with THF to remove DBU. Solvent was removed and the residue dried in vacuo for a 104.6 % yield of 8-methoxy^-nit^o-5'-succmimido-1^3^3f-t^imethyl-spi^o-[2H-l-benzopyran-2,2'-indoline].

19
We Claim:
1. A compound comprising the formula I:

wherein R1 is a C1-C4 alkyl, phenyl, benzyl, ally!, or ethoxycarbonyl ethyl, R2 and R3 are each independently meth'yl, ethyl, or phenyl, R4 is hydrogen, hydroxy, trichioromethyl, trifluoromethyl, formyl, C1-C4 alkyl, halogen, C1-C4 alkoxy, nitro, cyano, Ci-C4 monohaloalkyl, C1-C4 alkoxycarbonyl, or an aromatic sharing the two adjacent carbon atoms with the benzene portion of the pyran ring to form a condensed aromatic ring, and x equals 1, 2, or 3.
2. The compound as claimed in claim 1, wherein when x = 1, R4 is
located on any of the available carbon atoms of the benzene
ring of the benzopyran moiety.
3. The compound as claimed in claim 2, wherein R4 is located on
the 6, 7 or 8 position of the benzene ring of the benzopyran
moiety.

20
4. The compound as claimed in claim 1, wherein when x = 2, each
R4 is the same or different and located at the 6 and 8 or 5 and 7
positions of the benzene ring of the benzopyran moiety.
5. The compound as claimed in claim 3, wherein each R4 is located
at the 6 and 8 positions of the benzene ring of the benzopyran
moiety.
6. The compound as claimed in claim 1 comprising the formula:

wherein R4 is hydrogen, hydroxy, trifluoromethyl, formyl, methyl, ethyl, methoxy, ethoxy, nitro, fluoro, chloro, bromo, or iodo, and x is 1 or 2.
7, An article comprising a compound of formula I:

21

wherein R1 is a C1-C4 alkyl, phenyl, benzyl, atlyl, or ethoxycarbonyl ethyl, R2 and R3 are each independently methyl, ethyl, or phenyl and R4 is C1-C4 alkyl, C1-C2 alkoxy, chloro, bromo, iodo, trifluoromethyl, or nitro, R4 is hydrogen, hydroxy, trichtoromethyl, trifluoromethyl, formyl, C1-C4 alkyl, halogen, C1-C4 alkoxy, nitro, cyano, C1-C1 monohaloalkyl, C1-C4 alkoxycarbonyl, or an aromatic sharing the two adjacent carbon atoms with the benzene portion of the pyran ring to form a condensed aromatic ring, and x equals 1, 2, or 3.
8." The article as claimed in claim 6, wherein in the compound when x = 1, R4 is located on any of the available carbon atoms of the benzene ring of the benzopyran moiety.

22
9. The article as claimed in claim 8, wherein in the compound R4 is
located on the 6, 7, or 8 position of the benzene ring of the
benzopyran moiety.
10. The article as claimed in claim 6, wherein in the compound
when x = 2, each R4 be the same or different and located at the
6 and 8 or 5 and 7 positions of the benzene ring of the
benzopyran moiety.
11. The article as claimed in claim 10, wherein each R4 is located at
the 6 and 8 positions the benzene ring of the benzopyran
moiety.
12.The article as claimed in claim 6, wherein the article is an ophthalmic lens.
1 3. The article as claimed in claim 7 comprising a compound of the formula II:

23
wherein R4 is hydrogen, hydroxy, trifluoromethyl, formyl, methyl, ethyl, methoxy, ethqxy, nitro, fluoro, chloro, bromo, or iodo, and x is 1 or 2.
14. The article as claimed in claim 13, wherein the article is an
ophthalmic lens.
15. A method for producing an indolinospiropyran compound as
claimed in claim 1 comprising the steps of:
(i) providing an indoline loaded resin of the formula:

wherein R1 is a C1-C4 alkyl, phenyl, benzyl, allyl, or ethoxycarbonyl ethyl, R2 and R3 are each independently methyl, ethyl, or phenyl and R4 is C1-C4 alkyl, C1-C2 alkoxy, chloro, bromo, iodo, trifluoromethyl, or nitro;

24
(ii) treating the loaded resin at a temperature of 50°C to 120°C under an inert atmosphere for a time of 14 hours to 11 days, with 1.1. to 10 equivalents of a salicylaldehyde derivative of the formula VI:

wherein R4 is hydrogen, hydroxy, trichloromethyl, formyl, C1-C4 atkyl, halogen, C1-C4 alkoxy, nitro, cyano, C1-C1 monohaloalkyl, C1-C4 alkoxycarbonyl, or an aromatic sharing the two adjacent carbon atoms with the benzene portion of the pyran ring to form a condensed aromatic ring, and x equals 1, 2, or 3 to produce the indolinospiropyran compound; and (iii) releasing the indolinospiropyran compound.
16. The method as claimed in claim 15 wherein the resin is a hydroxy resin.

25
1 7. The method as claimed in claim 16, wherein the hydroxy resin is a hydroxymethyl polystyrene resin, a Wang resin, HMPA-PEGA resin, HMPB-BHA resin, HMPB-MBHA resin, or a combination thereof.
18. The method as claimed in claim 15, wherein the resin is of an 100-200 mesh or an 200-400 mesh.
W-rThe method as claimed in claim 17, wherein an 100-200 mesh, high loading hydroxymethyl polystyrene resin or a Wang resin is used.
20. The method as claimed in claim 1 5, further comprising the step of preparing the indoiine loaded resin by: (i) treating an aminoindoline compound of the formula

wherein R1 is a C1-C4 alky I, phenyl, benzyl, allyl, or ethoxycarbonyl ethyl, R2 and R3 are each independently methyl,

26
ethyl, or phenyl and R4 is C1-C4 alkyl, C1-C2 alkoxy, chloro,
bromo, iodo, trifluoromethyl, or nitro, with succinic acid at a
temperature of 0° to 60°C under inert atmosphere for 3 to 24
hours.
(ii) adding, subsequently to the mixture a hydroxy resin,
diisopropyl carbodiimide and dimethylamino pyridine to
form a suspension bead; and (iii) shaking the suspension bead at a temperature of 0°C to
60°C for 14 to 24 hours under inert atmosphere to form
the indoline loaded resin.
21. The method as claimed in claim 15, comprising preparing the
indoline loaded resin by:
(i) shaking an excess of hydroxy resin with an excess of succinic anhydride at a temperature of 60 to 120°C for 24 to 48 hours to couple the resin and succinic acid;
(ii) washing away, subsequently, excess succinic anhydride; and

27
(Mi) treating, with 1-hydroxy benzotriazole and DIC at 0 to 60°C and under an inert atmosphere, the coupled resin and succinic acid with an aminoindoline of the formula IV:

wherein R1 is a C1-C4 alkyl, phenyl, benzyl, allyl, or ethoxycarbonyl ethyl, R2 and R3 are each independently methyl, ethyl, or phenyl and R4 is C1-C4 alkyl, C1-C2 alkoxy, chloro, bromo, iodo, trifluoromethyl, or nitro.
22. The method as claimed in claim 20, comprising the steps of forming the aminoindoline compound by: reducing a nitroindoline derivative of the formula V:

wherein R1 is a C1-C4 alkyl, phenyl, benzyl, allyl, or ethoxycarbonyl ethyl, R2 and R3 are each independently methyl, ethyl, or phenyl and R4 is C1-C2 alkoxy, chioro, bromo, iodo, trifluoromethyl, or nitro.

28
23. The method as claimed in claim 21, comprising the steps of forming the aminoindoline compound by: reducing a nitroindoline derivative of the formula:

wherein R1 is a C1-C4 alkyl, phenyl, benzyl, allyl, or ethoxycarbonyl ethyl, R2 and R3 are each independently methyl, ethyl, or phenyl and R4 is C1-C2 alkoxy, chloro, bromo, iodo, trifluoromethyl, or nitro.
24. An indoline loaded resin usable in the method as claimed in claim 1 5 of the formula:

29
wherein R1 is a C1-C4 alkyl, phenyl, benzyl, allyl, or ethoxycarbonyl ethyl, R2 and R3 are each independently methyl, ethyl, or phenyl and R4 is C1-C2 alkoxy, chloro, bromo, iodo, trifluoromethyl, or nitro.
A compound comprising the formula:
wherein R1 is a C1-C4 alkyl, phenyl, benzyl, allyl, or ethoxycarbonyl ethyl, R2 and R3 are each independently methyl, ethyl, or phenyl, R4 is hydrogen, hydroxy, trichloromethyl, trifluoromethyl, formyl, C1-C4 alkyl, halogen, C1-C4 alkoxy, nitro, cyano, C1-C4 monohaloalkyl, C1-C4 alkoxycarbonyl, or an aromatic sharing the two adjacent carbon atoms with the benzene portion of the pyran ring to form a condensed aromatic ring, and x equals 1, 2, or 3.

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Patent Number

210119

Indian Patent Application Number

IN/PCT/2002/01452/KOL

PG Journal Number

38/2007

Publication Date

21-Sep-2007

Grant Date

18-Sep-2007

Date of Filing

26-Nov-2002

Name of Patentee

ESSILOR INTERNATIONAL (COMPAGNIE GENERALE D'OPTIQUE)

Applicant Address

147 RUE DE PARIS,F-94220 CHARENTON LE PONT,FRANCE.

Inventors:

#	Inventor's Name	Inventor's Address
1	CARREIRA ,ERICK,M.,	CHAPFSTRASSE 73,CH-8126 ZUMIKON,SWITZERLAND. U.SA.
2	WEILI,ZHAO,HIRZENBACHSTRASSE	77/8,CH-8051 ZURICH,SWITZERLAND,CHINESE NATIONAL

PCT International Classification Number

C09K 9/02

PCT International Application Number

PCT/US01/17018

PCT International Filing date

2001-05-24

PCT Conventions:

#	PCT Application Number	Date of Convention	Priority Country
1	09/585,108	2000-05-31	U.S.A.