Title of Invention	STABLE COMPLEXES OF POORLY SOLUBLE COMPOUNDS
Abstract	The present invention relates to stable water insoluble complexes of poorly soluble compounds molecularly dispersed in water-insoluble ionic polymers are disclosed Useful insoluble ionic polymers have a molecular weight greater than about 80,000 D and a glass transition temperature equal to or greater about 50°, The compounds are microprecipitated in the ionic polymers in amorphous form. The complexes according to the present invention significantly increase the bioavailability of poorly solubletherapeutically active compounds.

Title of Invention

STABLE COMPLEXES OF POORLY SOLUBLE COMPOUNDS

Abstract

The present invention relates to stable water insoluble complexes of poorly soluble compounds molecularly dispersed in water-insoluble ionic polymers are disclosed Useful insoluble ionic polymers have a molecular weight greater than about 80,000 D and a glass transition temperature equal to or greater about 50°, The compounds are microprecipitated in the ionic polymers in amorphous form. The complexes according to the present invention significantly increase the bioavailability of poorly solubletherapeutically active compounds.

Full Text	The present invention provides pharmaceutical compositions comprising a stable water-insoluble complex composed of an amorphous therapeutically active compound (e.g. a drug) dispersed in an ionic polymer. The complexes according to the present invention provide significant increases in bioavailability of poorly soluble therapeutically active compounds. The bioavailability of a therapeutically active compound is generally affected by (i) the solubility/dissolution rate of the compound, and (ii) the partition coefficient/permeability of the compound through a subject's gastrointestinal membrane. The major cause of poor bioavailability of a therapeutically active compound is the poor solubility/dissolution rate of said compound. Poor bioavailability is also often accompanied with undesirably high rates of patient variability and unpredictable dose/therapy effects due to erratic absorption of the therapeutically active compound (e.g. drug) by the patient. Several techniques are used to improve the bioavailability of poorly soluble therapeutically active compounds. These techniques are summarized below. 1. Particle Size Reduction: A poorly soluble therapeutically active compound often is mechanically ground to reduce the particle size of the compound and thereby increase the surface area. See Lachman et alM The Theory and Practice of Industrial Pharmacy, Chapter 2, p. 45 (1986). Particle size reduction into micron size particles can be achieved using a jet mill. The mean particle size obtained by the jet mill is typically in the range of 1-10 jxm. Similarly, wet milling of a therapeutically active compound in the presence of protective colloids or polymers typically yields particle sizes of compound in the range of about 300-800 nm. According to this technique, a therapeutically active compound and a polymer are dispersed in water and ground by DV/cp/13.07.1999 grinding media such as tiny beads (0.2-0.5 mm). See U.S. Patent No. 5,494,683. Particle size reduction however, can only.improve the dissolution rate of the therapeutically active compound, but not the total amount of compound in solution at equilibrium, 2, Solid Dispersion 2.1 Fusion method: According to this technique, a therapeutically active compound is dispersed into a non-ionic polymer to form a solid dispersion. Typically, the non-ionic polymer (e.g. Pluronic® and Polyethylene Glycol) is melted to a temperature above its melting point and the therapeutically active compound is dissolved, with stirring, into the molten polymer. See U.S. Patent No. 5,281,420. The resulting molten mass is then cooled to room temperature. As a result of this process, the therapeutically active compound is fused into the polymer and on cooling, precipitates out in amorphous form. The amorphous form of the compound generally has a faster dissolution rate then the initial crystalline form of the compound. Thus, by rendering the compound in amorphous form this process improves bioavailability. However, due to the greater aqueous solubility and low melting point of non-ionic polymers, the amorphous form of the therapeutically active compound, can not maintain its stability and eventually converts back to the crystalline form after exposure to high humidity and elevated temperatures often encountered during long term storage. See Yoshioka et al., J. Pharm. Sci. 83:1700-1705 (1994). Therefore, this technique is not suitable for most dosage forms of therapeutically active compounds, and certainly not for those therapeutically active compounds having poor solubility. 2.2 Co-precipitation: In another existing method for improving the bioavailability of a poorly soluble therapeutically active compound, the compound and a non-ionic hydrophilic polymer, such as polyvinyl pyrrolidone, are dissolved in an organic solvent. The solvent is removed by evaporation during which the therapeutically active compound precipitates into the hydrophilic polymer matrix. Seet H.G. Britain, Physical Characterization of Pharmaceutical Solids, Drugs and the Pharmaceutical Sciences, Vol. 70 (Marcel Dekker, Inc., N.Y., 1995). Due to the hygroscopic nature and aqueous solubility of the polymer, this type of polymer does not protect the amorphous form of the therapeutically active compound from heat and moisture. Thus, the therapeutically active compound in the hydrophilic polymer matrix does not stay in amorphous form and eventually converts to a crystalline form during storage. Therefore, this approach also is not practical to improve the bioavailability of poorly soluble therapeutically active compounds. 3. Self-Emulsifying Drug Delivery System (SEDDS): In this system, a therapeutically active compound is dissolved in a mixture of a suitable oil and emulsifier. The resultant lipid formulation, upon exposure to gastrointestinal fluids, forms a very fine emulsion or microemulsion. Due to high surface area of the oil globules, the bioavailability of a poorly soluble therapeutically active compound dissolved in such oil is significantly increased. See, P.P. Constantinides, Pharm. Res. 12(11): 1561-1572 (1995). The key requirement for use of this system is that the therapeutically active compound must be soluble in oil and once dissolved in oil, must remain in stable form in the solution. SEDDS is thus not a useful alternative for most therapeutically active compounds due to the limited solubility and unsatisfactory stability of these compounds in an oil-based solution. We have surprisingly found that when a poorly soluble therapeutically active compound (typically in crystalline form) is molecularly dispersed in a water-insoluble ionic polymer having a molecular weight greater than about 80,000 D and a glass transition temperature equal to or greater than about 50°C, the physical stability of the compound (now in amorphous form) is maintained for long periods of time even under high humidity and temperature storage conditions. Due to the high molecular weight and high glass transition temperature of the ionic polymer, as well as its relative insolubility in water, the ionic polymer immobilizes the therapeutically active compound in its amorphous form thereby providing excellent stability of compound which is superior to that afforded by currently available methods. In addition, due to the increased solubility of the compound in the compound/polymer complex, the bioavailability of the therapeutically active compound is also significantly increased. This method is therefore particularly useful for improving the bioavailability of poorly soluble therapeutically active compounds. The present invention provides a pharmaceutical composition comprising a stable, water-insoluble complex composed of a carrier macromolecule that is a water-insoluble ionic polymer having a molecular weight greater than about 80,000 D and a glass transition temperature equal to or greater than about 50°C, and an amorphous therapeutically active compound, wherein the therapeutically active compound is incorporated or dispersed in the ionic polymer in stable amorphous form to yield a compound/polymer complex. Another aspect of this invention is the water-insoluble compound/polymer complex. The complex of the invention is formed by the microprecipitation of the therapeutically active compound in the ionic carrier. The compound/polymer complex of the invention may be in the form of a solid (e.g. a paste, granules, a powder) which can be filled into capsules or compressed into tablets. The powdered form of the complex may also be pulverized or micronized sufficiently to form stable liquid suspensions or semi-solid dispersions. The complex of the invention may be sterilized, such as by gamma irradiation or electron beam irradiation, prior to administration in vivo for parenteral applications. This invention relates to a stable water-insoluble complex composed of a water-insoluble ionic polymer carrier having a molecular weight greater than about 80,000 D and a glass transition temperature equal to or greater than about 50°C and a therapeutically active compound in stable amorphous form. This invention also relates to methods of making such complexes and pharmaceutical formulations including such complexes. The advantage of the complexes of the invention include the ability to increase substantially the bioavailability of relatively insoluble therapeutically active compounds and the ability for delivery of such compounds for prolonged periods of time (that is, a sustained release of such compounds into the bloodstream). As used herein, the following terms shall have the following meanings. "Compound/polymer complex" or "water-insoluble complex" refer to a physically stable product that forms upon the concurrent precipitation ("microprecipitation") of a therapeutically active compound and a water-insoluble ionic polymer according to the methods described herein. "Dispersed" means random distribution of a therapeutically active compound throughout an ionic polymer. "Dissolution Rate" means the speed with which a particular compound dissolves in physiological fluids in vitro. "Ionic polymer* or "ionic carrier polymer" includes both anionic (negatively charged) and cationic (positively charged) polymers. "Microprecipitation" means any method by which a compound, in particular a therapeutically active compound, is molecularly dispersed in a polymer. "Molecularly dispersed" means that the therapeutically active compound(s) is present in the polymer in a final state of subdivision. See, e.g., M.G. Vachon et al., J. Microencapsulation 14(3): 281-301 (1997); MA and Vandelli et alM J. Microencapsulation 10(1): 55-65 (1993). "Patient" refers to a human subject. "Poorly soluble therapeutically active compound" refers to therapeutically active compounds (e.g. drugs) having an aqueous solubility of less than about 1 mg/mL, often less than about 100 jig/mL One aspect of the present invention pertains to pharmaceutical compositions comprising a stable water-insoluble complex composed of a carrier macromolecule that is an ionic polymer and a therapeutically active compound that is stable in its amorphous form. The use of such compound/polymer complex is particularly preferable when the compound is otherwise poorly soluble making it difficult to obtain desirable oral bioavailability of said compound. According to the present invention, when poorly soluble crystalline therapeutically active compound and a water-insoluble ionic polymer having a molecular weight greater than about 80,000 D and a glass transition temperature equal to or greater than about 50°C are microprecipitated, the compound is molecularly dispersed in amorphous form, into the ionic polymer producing a stable, water insoluble complex. Microprecipitation may be accomplished, for example, by any one of the following methods, each of which is further described infra: a) Spray Drying or Lyophilization Method b) Solvent-Controlled Precipitation c) pH-Controlled Precipitation d) Hot Melt Extrusion Process e) Supercritical Fluid Technology Once the therapeutically active compound is so dispersed in the ionic polymer, it retains its amorphous structure even during long term storage, that is, it is "stable". In addition, the ionic polymer protects the compound from detrimental external environmental factors such as moisture and heat, thereby retaining increased solubility and consequent increased bioavailability. A therapeutically active compound that is contained in a complex amorphous form according to the invention has significantly increase bioavailability in comparison to said compound in its crystalline form and is highly stable over a prolonged period of time. In addition, due to a controlled dissolution rate of the complex in the gastrointestinal fluids, the complex affords sustained release characteristics for the therapeutically active compound dispersed in the compound/polymer complex. This invention is useful with any therapeutically active compound, but is especially useful for therapeutically active compounds having aqueous solubilities of less than about 1 mg/mL, and especially for compounds having less then 100 p.g/mL Such poorly soluble therapeutically active compounds include, for example, retinoids and protease inhibitors. In particular, this invention is especially useful with the following therapeutic compounds: This invention is also useful with the compound tolcapone (marketed by Roche Laboratories Inc. under the brand name Tasmar®), the compound 1,3-cis-retinoic acid (commercially from available from Roche Laboratories Inc. under the brand name ACCUTANE®), the compound saquinavir (marketed by Roche Laboratories Inc. as FORTOVASE™), and with the following compounds: The ionic polymers suitable for use in accordance with this invention are either cationic or anionic polymers, have a molecular weight of above about 80,000 D, a glass transition temperature equal to or greater than about 50°C, are relatively insoluble in water and preferably have pH-dependent solubility. Examples of such polymers include polyacrylates (e.g. Eudragit®, Rohm America), chitosan, Carbopol® (BF Goodrich), polyvinyl acetate phthalate, cellulose acetate phthalate, polycyanoacrylates, hydroxypropylmethyl cellulose phthalate, cellulose acetate terphthalate, hydroxypropyl methyl cellulose acetyl succinate, carboxy methyl cellulose and low substituted hydroxy propyl cellulose. The water-insoluble complexes according to present invention may also be comprised of mixtures of two or more above-described ionic polymers (see, e.g. Examples 9 and 10). Particularly preferred anionic polymers include Eudragit® L100-55 (methacrylic acid and ethyl acrylate copolymer) and Eudragit® L100 or Eudragit® S100 (methacrylic acid and methyl methacrylate copolymers), all of which are available from Rohm America. Eudragit® L100-55 is soluble at a pH above 5.5 and practically insoluble at pH below 5.5. The molecular weight of Eudragit® L100-55 is approximately 250,000 D and the glass transition temperature is 110°C. Eudragit® L100 is soluble at pH above 6 and practically insoluble at pH below 6. The molecular weight of Eudragit® L100 is approximately 135,000 D and the glass transition temperature is about 150°C, Eudragit® S100 is soluble at pH above 7 and practically insoluble at pH below 7. The molecular weight of Eudragit® S100 is approximately 135,000 D and the glass transition temperature is about 160°C. Particularly preferred cationic polymers include Eudragit® E (Rohm America), which is a copolymer of dimethylaminoethylmethacrylate and neutral methacrylic esters. This polymer is soluble up to pH 4 and is practically insoluble at a pH above 4. The molecular weight of Eudragit® E is approximately 150,000 D and the glass transition temperature is about 50°C. Pharmaceutical compositions of the present invention Comprising the water-insoluble complexes of the invention may be manufactured in a manner that is known in the art, e.g. by means of conventional mixing, milling, encapsulating, dissolving, compressing, granulating, or lyophilizing processes. In addition to the water-insoluble complexes, these pharmaceutical compositions may also include therapeutically inert, inorganic or organic carriers ("pharmaceutically acceptable carriers"), other than the ionic polymer, and/or excipients. Pharmaceutically acceptable carriers for tablets, coated tablets, dragees and hard gelatin capsules include lactose, maize starch or derivatives thereof, talc, stearic acid or its salts. Suitable carriers for soft gelatin capsules include vegetable oils, waxes, fats, and semi-solid or liquid polyols. The pharmaceutical compositions of the invention may also contain preserving agents, solubilizing agents, stabilizing agents, wetting agents, emulsifying agents, sweetening agents, coloring agents, flavoring agents, salts for varying the osmotic pressure, buffers, coating agents or antioxidants. These compositions may also contain additional therapeutically active compounds or more than one therapeutically active compound/polymer complex. Methods of Preparation In one embodiment of the present invention, water-insoluble complexes of the invention are prepared using one of the following methods: a) Spray Drying or Lyophilizatlon Method: The therapeutically active compound and the ionic polymer are dissolved in a common solvent having a low boiling point, e.g., ethanol, methanol, acetone, etc. By means of spray drying or lyophilization, the solvent is evaporated, leaving the therapeutically active compound microprecipitated in amorphous form in the ionic polymer matrix. This technique is not preferable for those therapeutically active compounds that do not have adequate solubility (>5%) in the preferred solvents. b) Solvent Controlled Precipitation: The therapeutically active compound and the ionic polymer are dissolved in a common solvent, e.g., dimethylacetamide, dimethylformamide, etc. The therapeutically active compound/ polymer solution is added to cold (2°-5°C) water adjusted to appropriate pH. The desired pH is dependent on the polymer used and is readily asertainablebly one skilled in the art. This causes the therapeutically active compound to microprecipitate in the polymer matrix. The microprecipitate is washed several times with aqueous medium until the residual solvent falls below an acceptable limit for that solvent. An "acceptable limit" for each solvent is determined pursuant to the International Conference on Harmonization (ICH) guidelines. c) pH-Controlled Precipitation: In this process, microprecipitation of the therapeutically active compound in an ionic polymer is controlled by a drastic change in pH of the solution. The therapeutically active compound and the ionic polymer are dissolved at a high pH (e.g. pH ~ 9) and precipitated by lowering the pH of the solution (e.g. to - 1), or vice versa. This method is particularly suitable for therapeutically active compounds that have pH-dependent solubility. d) Hot Melt Extrusion Process: Mircroprecipitation of a therapeutically active compound in an ionic polymer having thermoplastic characteristics can be achieved by a hot melt extrusion process. The crystalline therapeutically active compound and the polymer are mixed in a suitable blender and fed continuously to a temperature-controlled extruder causing the therapeutically active compound to be molecularly dispersed in the molten ionic polymer. The resulting extrudates are cooled to room temperature and milled into a fine powder. e) Supercritical Fluid Technology: The therapeutically active compound and an ionic polymer are dissolved in a supercritical fluid such as liquid nitrogen or liquid carbon dioxide. The supercritical fluid is then removed by evaporation leaving the therapeutically active compound microprecipitated in the polymer matrix. In another method, the therapeutic compound and an ionic polymer is dissolved in a suitable solvent. A microprecipitated powder can then be formed by spraying the solution in a supercritical fluid which acts as an antisolvent. In another embodiment of the invention, pharmaceutical formulations may be prepared according to any one of the foregoing steps by the addition of a final step during which the compound/polymer complexes of the invention are formulated by methods well-known in the art. In a preferred embodiment of the invention, the therapeutically active compound and the ionic polymer are dissolved in an organic solvent. Thereafter, the compound and the ionic polymer are co-precipitated relatively concurrently, preferably in aqueous solution, and preferably at a pH where, independently, neither the compound nor the polymer are soluble. The organic solvent used to dissolve the therapeutically active compound and the ionic polymer should provide good solubility for both the poorly soluble compounds and the polymers used. These solvents include ethyl alcohol, methyl alcohol, acetone dimethyl sulfoxide, dimethyl acetamide, dimethyl formamide, N-methylpyrrolidone, Transcutol® (Diethylene glycol monoethyl ether, Gattefosse, Inc.), glycofural, propylene carbonate, tetrahydrofuran, polyethylene glycols and propylene glycols. The pH selected to co-precipitate the therapeutically active compound and the ionic polymer depends on the solubility of each of the specific polymers and compounds being precipitated. One skilled in the art can easily ascertain the preferred pH for co-precipitation for each combination of polymer and therapeutically active compound. In a preferred embodiment wherein an anionic polymer selected from Eudragit® L100-55, Eudragit® L100 and Eudragit® S100 is used, the solution is precipitated at a pH lower than about 4. In another preferred embodiment wherein the cationic polymer Eudragit® E100 is used, the solution is precipitated preferably at a pH above 4. The amounts of therapeutically active compound(s) and polymer necessary to achieve the stable, water-insoluble complex of the invention may vary depending upon the particular compound and ionic polymer(s) used, as well as the particular solvent(s) and precipitation parameters. By way of example, the compound may be present in the complex from about 0.1% to about 80%, by weight. Analogously, the polymer is typically present in the complex in not less than about 20% by weight. Preferably, the compound is present in the complex from about 30% to about 70% by weight, more preferably from about 40% to about 60% by weight. Most preferably, the compound is present in the complex at about 50% by weight. For a complex incorporating Compound I, the compound is present in the complex at about 30-70% by weight, most preferably at about 50% by weight. Once the compound/polymer complex precipitates out of solution, the resulting complex can be recovered from the solution by procedures known to those skilled in the art, for example by filtration, centrifugation, washing, etc. The recovered mass can then be dried (in air, an oven, or a vacuum) and the resulting solid can be milled, pulverized or micronized to a fine powder by means known in the art. The powder form of the complex can then be dispersed in a carrier to form a pharmaceutical preparation. The pharmaceutical preparations according to the invention can be administered to a subject by any route suitable for achieving the desired therapeutic result(s). Preferred routes of administration include parenteral and oral administration. The pharmaceutical formulations according to the invention include a therapeutically effective amount of a therapeutically active compound. A therapeutically effective amount means an amount, at such dosages and for such periods of time, necessary to achieve the desired therapeutic result. Moreover, such amount must be one in which the overall therapeutically beneficial effects outweigh the toxic or undesirable side effects. A therapeutically effective amount of a compound often varies according to disease state, age and weight of the subject being treated. Thus, dosage regimens are typically adjusted to the individual requirements in each particular case and are within the skill in the art. By way of example, for Compound I above, the appropriate daily dose for administration to an adult human weighing about 70 kg is from about 10 mg to about 10,000 mg, preferably from about 200 mg to about 1,000 mg, although the upper limit may be exceeded when indicated. The daily dosage of the therapeutically active compound can be administered as a single dose, in divided doses, or for parenteral administration, it may be given as subcutaneous injection. The examples which follow refer to appended drawings wherein FIG 1 is a powder x-ray diffraction pattern of the compound/polymer complex of Example 4 compared to the bulk drug alone and compared to drug and polymer physical mixture. FIG. 2 is a powder x-ray diffraction pattern of samples from the compound/polymer complex of Example 4 exposed to accelerated stress conditions compared to unstressed (initial) compound/polymer complex. FIG 3 is a plasma concentration profile in dogs of the compound/polymer complex of Example 4. FIG 4 is a powder x-ray diffraction pattern for Compound II "as is" and as compound/polymer complex (Example 11) after microprecipitation in accordance with the invention. FIG 5 is a powder x-ray diffraction pattern for Compound III "as is" and as compound/polymer complex (Example 13) after microprecipitation in accordance with the invention. FIG 6 is a powder x-ray diffraction pattern for Compound IV "as is" and as compound/polymer complex (Example 15) after microprecipitation in accordance with the invention. FIG 7 is a powder x-ray diffraction pattern for Compound V "as is" and as compound/polymer complex (Example 16) after microprecipitation in accordance with the invention. EXAMPLES The following examples illustrate methods for making the water-insoluble compound/polymer(s) complexes of the present invention as well as pharmaceutical preparations incorporating said complexes. For the examples reported herein, the therapeutically active compounds tested were Compounds I, ll} III, IV and V, the structures for which are provided above. These compounds are practically insoluble in gastrointestinal fluids. Prior to the current invention, the crystalline, insoluble form of Compound I was the only stable form of this Compound that could be obtained. General Procedures Procedure Applicable to Example 1 (micronized compound) Compound I was micronized using a fluid energy mill to yield an average particle size of 10 microns. This procedure did not alter the crystalline form of the compound. Procedure Applicable to Example 2 (nanosized compound) A 10% suspension of Compound I was wet milled in aqueous medium containing 5% Klucel EF® (Hydroxypropylcellulose, Aqualon Corp.) as a protective colloid to prevent aggregation. The milling was performed in batch mode in a Dynomill for 24 hours using 0.25 mm glass beads as milling media. The average particle size of the resultant suspension was 700 nm and the residue obtained after drying the suspension demonstrated that the compound was present in crystalline form, Procedure Applicable to Example 3 (Pluronic F 68 dispersion) A 10% dispersion of Compound I in 90% Pluronic F68 (polymer) was prepared using hot-melt technique. The compound was mixed into molten Pluronic F68 at 60°C and the dispersion was then heated up to 180°C to dissolve Compound I. The solution was cooled to room temperature to yield a solid mass. The powder x-ray diffraction ("XRD") pattern of the molten dispersion was similar to that for Pluronic F68. This XRD shows that Compound I was thus present in the solid dispersion in amorphous form. The solid dispersion obtained by this technique was further dispersed in aqueous medium prior to use in dosing animals. Procedure Applicable to Examples 412 and 15-16 (Molecular dispersion according to the invention) In accordance with the method of the invention, compounds I, II, IV or V and the specific polymer identified in each instance (i.e., Eudragit® L100-55, Eudragit® L100 or Eudragit® S100) were dissolved in dimethylacetamide. The resulting solution was then slowly added to cold (2-10°C) aqueous solution at pH 2 causing the compound and the polymer to co-precipitate as an insoluble matrix wherein the compound was molecularly dispersed in the polymer. In each case, the precipitate was washed several times with cold (2-10°C) aqueous solution at pH 2 until the residual dimethylacetamide was below 0.2%. The precipitate was dried in a forced air oven at 40°C for 24 hours to a moisture level of below 2% and milled using a Fitz Mill (Fitzpatrick) at slow speed using forward knives and size 0 screen into desirable particle sizes. The desired mean particle size was 90% particles in the size range 50-400 jim. Procedure applicable to Examples 13-14 (Compound III) In accordance with the methods described above, Compound III and a specific polymer identified in each instance (i.e., Eudragit® L100-55, Eudragit® L100, Hydroxypropylmethylcellulose phthalate (HP-50) or Eudragit® S100) were dissolved in ethanol. The resulting solution was either dried in a vacuum oven at 40°C for 24 hours until the weight loss on drying was less than 2%, or alternatively, the solution was spray dried. As a result of this process, the compound and the polymer co-precipitated as an insoluble complex wherein the compound was molecularly dispersed in the polymer. The resulting dried film was ground with a pestle/mortar and screened through 60 mesh screen. Data Table 1 below summarizes the results of Examples 1-16. Table 1 specifies the individual therapeutically active compounds and, where applicable, the compound/polymer complex that was prepared, the method of preparing the compound/polymer complex, and the physical characteristics of the resulting products from each example. As is shown in Figure 1 and Table 1, the powder x-ray diffraction (XRD) pattern of the complex resulting in Example 4, (Table 1), that is when Compound I is included in an ionic polymer in accordance with the process of the current invention, it takes an amorphous form. Table I and Figures 4-7 also show that the methods of the present invention are useful in rendering Compounds II, III, IV and V in amorphous form. The inclusion of Compound I in the ionic polymer protected the compound from external environmental effects such as moisture and heat. This result is demonstrated in Figure 2, wherein it is shown, by powder x-ray diffraction, that Compound I embedded in the polymer maintained its amorphous properties even under accelerated storage conditions. The ability of the complex to maintain Compound I in amorphous form even after storage at accelerated stress condition is due to the high molecular weight (> 80,000), high glass transition temperature (> 50°C) and insolubility in water of the polymer(s). Furthermore, as is shown in Table 2 below, the bioavailability in dogs of Compound I when it is molecularly dispersed in an ionic polymer in accordance with the invention was unexpectedly higher than when the compound was administered to the animals in conventional forms (e.g. micronized and wet milled). Also shown in Table 2 are the bioavailability results obtained from solid dispersion of Compound I prepared by hot-melt method with Pluronic F68® (non-ionic water soluble polymer containing poly-oxyethyiene and polyoxypropylene chains, BASF). While the biovailability of the Compound in this solid dispersion was better than when the compound was micronized or in wet mill suspension, the physical stability of the solid dispersion was not satisfactory for a pharmaceutical product as is evident by the reversion of compound to its crystalline form within one month of storage at ambient conditions. The above-described results demonstrate the unsuitability for preparation of a pharmaceutical product of the solid dispersion technique in non-ionic water soluble polymers. Results are mean values (with standard deviations) for four animals (2 males and 2 females). Compared to Single Dose Intravenous Administration. * Converts to crystalline form after exposure to 40°C, 75%RH, 1 wk, open condition. Figure 3 shows plasma concentration-time profile of different batches of the compound/polymer complex produced in accordance to Example 4. The results of these tests (summarized in Figure 3), show batch to batch reproducibility and consistency. Batch to batch reproducibility and consistency is an important aspect of any formulation that is intended for administration to human patients. Figures 4-7 show that Compounds II, III, IV and V also can be converted into amorphous form using this invention. In summary, as is shown by the data in Tables 1 and 2 above and in Figures 1, 2, and 4-7, the powder x-ray diffraction patterns of the compound/polymer(s) complexes obtained in Examples 4-16 shows that molecularly dispersing a poorly soluble compound in an ionic polymer(s) according to the present invention converts the compounds into amorphous form and maintains excellent stability of the amorphous compound upon long-term storage. CLAIMS 1. A pharmaceutical composition comprising a water-insoluble complex of a therapeutically active, stable amorphous compound and a water-insoluble ionic polymer that has a molecular weight greater than about 80,000 D and a glass transition temperature equal to or greater than about 50°C, and a carrier. 2. The pharmaceutical composition of claim 1 wherein the therapeutically active compound is a compound that is poorly soluble when in crystalline form. 3. The pharmaceutical composition of claim 2 wherein the poorly soluble therapeutically active compound in its crystalline form has a solubility of less than 1 mg/mL in aqueous solution, 4. The pharmaceutical composition of claim 1 wherein the ionic polymer is a cationic polymer. 5. The pharmaceutical composition of claim 4 wherein the cationic polymer is a copolymer of dimethylaminoethylmethacrylate and neutral methacrylic ester. 6. The pharmaceutical composition of claim 5 wherein the cationic polymer is Eudragit E®. 7. The pharmaceutical composition of claim 1 wherein the ionic polymer is an anionic polymer. 8. The pharmaceutical composition of claim 7 wherein the anionic polymer is a copolymer of methacrylic acid and ethyl acrylate or methacrylic acid and methyl methacrylate. 9. The pharmaceutical composition of claim 8 wherein the anionic polymer is selected from the group consisting of Eudragit L 100-55® , Eudragit MOO® and Eudragit S-100®. 10. The pharmaceutical composition of claim 7 wherein the anionic polymer is selected from the group consisting of polyvinyl acetate phthalate, cellulose acetate phthalate, hydroxypropylmethylcellulose phthalate, cellulose acetate terphthalate, polycyanoacrylate and hydroxypropyl methyl cellulose acetyl succinate, carboxy methyl cellulose, and low substituted hydroxypropylcellulose. 11. The pharmaceutical composition of claim 1 wherein the solubility of the ionic polymer is pH dependent. 12. The pharmaceutical composition of claim 11 wherein the ionic polymer is insoluble at pH above about 4. 13. The pharmaceutical composition of claim 1 wherein the ionic polymer and the therapeutically active compound in its crystalline form are both relatively insoluble above pH of about 4. 14. The pharmaceutical composition of claim 11 wherein the ionic polymer is insoluble at pH below about 4. 15. The pharmaceutical composition of claim 1 wherein the ionic polymer and the therapeutically active compound in its crystalline form are both relatively insoluble at pH below about 4. 16. The pharmaceutical composition of claim 1 wherein the therapeutically active compound is selected from the group consisting of Compounds I, II, III, IV, V, VI, VII and VIII. 17. A pharmaceutical composition comprising a water-insoluble complex of Compound I in stable amorphous form and a water-insoluble ionic polymer that has a molecular weight greater than about 80,000 D and a glass transition temperature equal to or greater than about 50°C, and a carrier. 18. The pharmaceutical composition of claim 1 wherein the ionic polymer is present in the water-insoluble complex at not less than about 20%, by weight. 19. The pharmaceutical composition of claim 18 wherein the therapeutically active compound is present in the water-insoluble complex at about 0.1% to about 80%, by weight of said complex, 20. The pharmaceutical composition of claim 19 wherein the therapeutically active compound is present in the water-insoluble complex at about 30% to about 70%, by weight of said complex. 21. The pharmaceutical composition of claim 20 wherein the ionic polymer is present in the water-insoluble complex at about 50%, by weight, and the therapeutically active compound is present at about 50%, by weight of said complex. 22. A method for preparing a pharmaceutical formulation comprising a water-insoluble complex of a stable, amorphous therapeutically active compound and an ionic polymer comprising: (a) dissolving the therapeutically active compound and ionic polymer in a suitable solvent; (b) contacting the solution of step (a) with an aqueous solution at a pH in which the ionic polymer is poorly soluble thereby microprecipitation the therapeutically active compound and ionic polymer as a compound/polymer complex; (c) preparing a pharmaceutical formulation that includes the compound/ polymer complex of step (b) above. 23. The method of claim 22, wherein in step (a), the therapeutically active compound and the ionic polymer are dissolved in a solvent selected from the group consisting of ethyl alcohol, methyl alcohol, dimethylsulfoxide, dimethylacetamide, dimethyl formamide, N-methylpyrrolidone, Transcutol® (diethylene glycol monoethyl ether, Gattefosse), glycofural, propylene carbonate, tetrahydrofuran, polyethylene glycol and propylene glycol, 24. The method in claim 22 wherein in step (b), microprecipitation is carried out by removing the solvent by spray drying or lyophilizing. 25. The method of claim 22 wherein in step (a), the insoluble therapeutically active and the ionic compound polymer are dissolved by adjusting the pH, 26. The method of claim 22 wherein after step (b), residual solvent is removed. 27. The method of claim 26 wherein the residual solvent is removed by washing the compound/polymer complex, 28. The method of claim 26 wherein the residual solvent is removed by evaporation or drying. 29. The method of claim 28 wherein the residual solvent is removed by spray drying. 30. A method for preparing a pharmaceutical formulation comprising a water-insoluble complex of a stable, amorphous therapeutically active compound and an ionic polymer comprising: (a) dissolving the therapeutically active compound, in its crystalline form, and ionic polymer in an organic solvent; (b) contacting the product of step (a) with an aqueous solution at a pH at which the ionic polymer and the .therapeutically active compound will precipitate as a compound/polymer matrix; (c) washing the compound/polymer matrix; (d) drying the compound/polymer matrix; and (e) preparing a pharmaceutical formulation that incorporates the washed and dried compound/polymer matrix of step (d) above. 31. The method of claim 30 wherein the therapeutically active compound that is incorporated in the compound/polymer matrix is predominantly in amorphous form. 32. The method of claim 31 wherein the ionic polymer is selected from the group consisting of Eudragit® E100, Eudragit® L100, Eudragit® L100-55 and Eudragit® S100. 33. A method for preparing a water-insoluble complex of a stable amorphous compound and an ionic polymer comprising: (a) melting together the therapeutically active compound and the ionic polymer; and (b) cooling the mixture resulting from step (a). 34. A method for preparing a pharmaceutical formulation comprising a water-insoluble complex of a stable amorphous compound and an ionic polymer comprising: (a) dissolving the therapeutically active compound and the ionic polymer in a supercritical fluid; (b) removing the supercritical fluid resulting in the microprecipitation of the therapeutically active compound in the polymer matrix; and (c) preparing a pharmaceutical^ formulation that includes the product of step (b) above. 35. The method of claim 34 wherein the supercritical fluid used in step (a) is selected from the group consisting of liquid nitrogen and liquid carbon dioxide. 36. The method of claim 34 wherein removal of the supercritical fluid in step (b) is accomplished by evaporation. 37. The method of claim 22, 30, 33, 34 or 35 wherein the therapeutically active compound is selected from the group consisting of Compounds I, II, III, IV, V, VI, VII and VIII, 38. A stable, water-insoluble complex prepared by (a) dissolving Compound I and a water-insoluble ionic polymer having a molecular weight greater than 80,000D and a glass transition temperature equal to or greater than 50°C in a suitable solvent; and (b) co-precipitating Compound I and the ionic polymer as a compound/polymer complex. 39. The complex of claim 38 wherein precipitation in step (b) in effected by contacting the solution of step (a) with an aqueous solution at a pH in which the ionic polymer is poorly soluble. 40. A water-insoluble complex comprising a stable, amorphous compound and a water-insoluble ionic polymer that has a molecular weight greater than 80,000 D and a glass transition temperature equal to or greater than 50°C. 41. The complex of claim 40 wherein the amorphous compound is poorly soluble in crystalline form. 42. A water-insoluble complex comprising Compound I in stable amorphous form and a water-insoluble ionic polymer that has a molecular weight greater than 80,000 D and a glass transition temperature equal to or greater than 50°C. 43, A method for stabilizing an amorphous compound comprising molecularly dispersing the compound in a water-insoluble ionic polymer that has a molecular weight greater than 80,000 D and a glass transition temperature equal to or greater than 50°C. 44. A method for converting a poorly soluble, crystalline compound to a stable amorphous form of said compound comprising molecularly dispersing said compound in a water-insoluble ionic polymer that has a molecular weight greater than 80,000 D and a glass transition temperature equal to or greater than 50°C. 45, A therapeutically active compound in stable amorphous form molecularly dispersed in a water-insoluble ionic polymer that has a molecular weight greater than 80,000 D and a glass transition temperature equal to or greater than 50°C. 46. The invention as herein before described.

Full Text

The present invention provides pharmaceutical compositions comprising a stable water-insoluble complex composed of an amorphous therapeutically active compound (e.g. a drug) dispersed in an ionic polymer. The complexes according to the present invention provide significant increases in bioavailability of poorly soluble therapeutically active compounds.
The bioavailability of a therapeutically active compound is generally affected by (i) the solubility/dissolution rate of the compound, and (ii) the partition coefficient/permeability of the compound through a subject's gastrointestinal membrane. The major cause of poor bioavailability of a therapeutically active compound is the poor solubility/dissolution rate of said compound. Poor bioavailability is also often accompanied with undesirably high rates of patient variability and unpredictable dose/therapy effects due to erratic absorption of the therapeutically active compound (e.g. drug) by the patient.
Several techniques are used to improve the bioavailability of poorly soluble therapeutically active compounds. These techniques are summarized below.
1. Particle Size Reduction: A poorly soluble therapeutically active compound often is mechanically ground to reduce the particle size of the compound and thereby increase the surface area. See Lachman et alM The Theory and Practice of Industrial Pharmacy, Chapter 2, p. 45 (1986). Particle size reduction into micron size particles can be achieved using a jet mill. The mean particle size obtained by the jet mill is typically in the range of 1-10 jxm. Similarly, wet milling of a therapeutically active compound in the presence of protective colloids or polymers typically yields particle sizes of compound in the range of about 300-800 nm. According to this technique, a therapeutically active compound and a polymer are dispersed in water and ground by DV/cp/13.07.1999

grinding media such as tiny beads (0.2-0.5 mm). See U.S. Patent No. 5,494,683. Particle size reduction however, can only.improve the dissolution rate of the therapeutically active compound, but not the total amount of compound in solution at equilibrium,
2, Solid Dispersion
2.1 Fusion method: According to this technique, a therapeutically active
compound is dispersed into a non-ionic polymer to form a solid dispersion. Typically,
the non-ionic polymer (e.g. Pluronic® and Polyethylene Glycol) is melted to a
temperature above its melting point and the therapeutically active compound is dissolved, with stirring, into the molten polymer. See U.S. Patent No. 5,281,420. The resulting molten mass is then cooled to room temperature. As a result of this process, the therapeutically active compound is fused into the polymer and on cooling, precipitates out in amorphous form. The amorphous form of the compound generally has a faster dissolution rate then the initial crystalline form of the compound. Thus, by rendering the compound in amorphous form this process improves bioavailability. However, due to the greater aqueous solubility and low melting point of non-ionic polymers, the amorphous form of the therapeutically active compound, can not maintain its stability and eventually converts back to the crystalline form after exposure to high humidity and elevated temperatures often encountered during long term storage. See Yoshioka et al., J. Pharm. Sci. 83:1700-1705 (1994). Therefore, this technique is not suitable for most dosage forms of therapeutically active compounds, and certainly not for those therapeutically active compounds having poor solubility.
2.2 Co-precipitation: In another existing method for improving the bioavailability
of a poorly soluble therapeutically active compound, the compound and a non-ionic
hydrophilic polymer, such as polyvinyl pyrrolidone, are dissolved in an organic
solvent. The solvent is removed by evaporation during which the therapeutically
active compound precipitates into the hydrophilic polymer matrix. Seet H.G. Britain,
Physical Characterization of Pharmaceutical Solids, Drugs and the Pharmaceutical

Sciences, Vol. 70 (Marcel Dekker, Inc., N.Y., 1995). Due to the hygroscopic nature and aqueous solubility of the polymer, this type of polymer does not protect the amorphous form of the therapeutically active compound from heat and moisture. Thus, the therapeutically active compound in the hydrophilic polymer matrix does not stay in amorphous form and eventually converts to a crystalline form during storage. Therefore, this approach also is not practical to improve the bioavailability of poorly soluble therapeutically active compounds.
3. Self-Emulsifying Drug Delivery System (SEDDS): In this system, a therapeutically active compound is dissolved in a mixture of a suitable oil and emulsifier. The resultant lipid formulation, upon exposure to gastrointestinal fluids, forms a very fine emulsion or microemulsion. Due to high surface area of the oil globules, the bioavailability of a poorly soluble therapeutically active compound dissolved in such oil is significantly increased. See, P.P. Constantinides, Pharm. Res. 12(11): 1561-1572 (1995). The key requirement for use of this system is that the therapeutically active compound must be soluble in oil and once dissolved in oil, must remain in stable form in the solution. SEDDS is thus not a useful alternative for most therapeutically active compounds due to the limited solubility and unsatisfactory stability of these compounds in an oil-based solution.
We have surprisingly found that when a poorly soluble therapeutically active compound (typically in crystalline form) is molecularly dispersed in a water-insoluble ionic polymer having a molecular weight greater than about 80,000 D and a glass transition temperature equal to or greater than about 50°C, the physical stability of the compound (now in amorphous form) is maintained for long periods of time even under high humidity and temperature storage conditions. Due to the high molecular weight and high glass transition temperature of the ionic polymer, as well as its relative insolubility in water, the ionic polymer immobilizes the therapeutically active compound in its amorphous form thereby providing excellent stability of compound which is superior to that afforded by currently available methods. In addition, due to the increased solubility of the compound in the compound/polymer complex, the

bioavailability of the therapeutically active compound is also significantly increased. This method is therefore particularly useful for improving the bioavailability of poorly soluble therapeutically active compounds.
The present invention provides a pharmaceutical composition comprising a stable, water-insoluble complex composed of a carrier macromolecule that is a water-insoluble ionic polymer having a molecular weight greater than about 80,000 D and a glass transition temperature equal to or greater than about 50°C, and an amorphous therapeutically active compound, wherein the therapeutically active compound is incorporated or dispersed in the ionic polymer in stable amorphous form to yield a compound/polymer complex. Another aspect of this invention is the water-insoluble compound/polymer complex. The complex of the invention is formed by the microprecipitation of the therapeutically active compound in the ionic carrier.
The compound/polymer complex of the invention may be in the form of a solid (e.g. a paste, granules, a powder) which can be filled into capsules or compressed into tablets. The powdered form of the complex may also be pulverized or micronized sufficiently to form stable liquid suspensions or semi-solid dispersions. The complex of the invention may be sterilized, such as by gamma irradiation or electron beam irradiation, prior to administration in vivo for parenteral applications.
This invention relates to a stable water-insoluble complex composed of a water-insoluble ionic polymer carrier having a molecular weight greater than about 80,000 D and a glass transition temperature equal to or greater than about 50°C and a therapeutically active compound in stable amorphous form. This invention also relates to methods of making such complexes and pharmaceutical formulations including such complexes. The advantage of the complexes of the invention include the ability to increase substantially the bioavailability of relatively insoluble therapeutically active compounds and the ability for delivery of such compounds for prolonged periods of time (that is, a sustained release of such compounds into the bloodstream).

As used herein, the following terms shall have the following meanings.
"Compound/polymer complex" or "water-insoluble complex" refer to a physically stable product that forms upon the concurrent precipitation ("microprecipitation") of a therapeutically active compound and a water-insoluble ionic polymer according to the methods described herein.
"Dispersed" means random distribution of a therapeutically active compound throughout an ionic polymer.
"Dissolution Rate" means the speed with which a particular compound dissolves in physiological fluids in vitro.
"Ionic polymer* or "ionic carrier polymer" includes both anionic (negatively charged) and cationic (positively charged) polymers.
"Microprecipitation" means any method by which a compound, in particular a therapeutically active compound, is molecularly dispersed in a polymer.
"Molecularly dispersed" means that the therapeutically active compound(s) is present in the polymer in a final state of subdivision. See, e.g., M.G. Vachon et al., J. Microencapsulation 14(3): 281-301 (1997); MA and Vandelli et alM J. Microencapsulation 10(1): 55-65 (1993).
"Patient" refers to a human subject.
"Poorly soluble therapeutically active compound" refers to therapeutically active compounds (e.g. drugs) having an aqueous solubility of less than about 1 mg/mL, often less than about 100 jig/mL

One aspect of the present invention pertains to pharmaceutical compositions comprising a stable water-insoluble complex composed of a carrier macromolecule that is an ionic polymer and a therapeutically active compound that is stable in its amorphous form. The use of such compound/polymer complex is particularly preferable when the compound is otherwise poorly soluble making it difficult to obtain desirable oral bioavailability of said compound.
According to the present invention, when poorly soluble crystalline therapeutically active compound and a water-insoluble ionic polymer having a molecular weight greater than about 80,000 D and a glass transition temperature equal to or greater than about 50°C are microprecipitated, the compound is molecularly dispersed in amorphous form, into the ionic polymer producing a stable, water insoluble complex. Microprecipitation may be accomplished, for example, by any one of the following methods, each of which is further described infra:
a) Spray Drying or Lyophilization Method
b) Solvent-Controlled Precipitation
c) pH-Controlled Precipitation
d) Hot Melt Extrusion Process
e) Supercritical Fluid Technology
Once the therapeutically active compound is so dispersed in the ionic polymer, it retains its amorphous structure even during long term storage, that is, it is "stable". In addition, the ionic polymer protects the compound from detrimental external environmental factors such as moisture and heat, thereby retaining increased solubility and consequent increased bioavailability.
A therapeutically active compound that is contained in a complex amorphous form according to the invention has significantly increase bioavailability in comparison to said compound in its crystalline form and is highly stable over a prolonged period of time. In addition, due to a controlled dissolution rate of the complex in the gastrointestinal fluids, the complex affords sustained release characteristics for the therapeutically active compound dispersed in the compound/polymer complex.

This invention is useful with any therapeutically active compound, but is especially useful for therapeutically active compounds having aqueous solubilities of less than about 1 mg/mL, and especially for compounds having less then 100 p.g/mL
Such poorly soluble therapeutically active compounds include, for example, retinoids and protease inhibitors. In particular, this invention is especially useful with the following therapeutic compounds:

This invention is also useful with the compound tolcapone (marketed by Roche Laboratories Inc. under the brand name Tasmar®), the compound 1,3-cis-retinoic acid (commercially from available from Roche Laboratories Inc. under the brand name ACCUTANE®), the compound saquinavir (marketed by Roche Laboratories Inc. as FORTOVASE™), and with the following compounds:

The ionic polymers suitable for use in accordance with this invention are either cationic or anionic polymers, have a molecular weight of above about 80,000 D, a glass transition temperature equal to or greater than about 50°C, are relatively insoluble in water and preferably have pH-dependent solubility. Examples of such polymers include polyacrylates (e.g. Eudragit®, Rohm America), chitosan, Carbopol®
(BF Goodrich), polyvinyl acetate phthalate, cellulose acetate phthalate, polycyanoacrylates, hydroxypropylmethyl cellulose phthalate, cellulose acetate terphthalate, hydroxypropyl methyl cellulose acetyl succinate, carboxy methyl cellulose and low substituted hydroxy propyl cellulose. The water-insoluble complexes according to present invention may also be comprised of mixtures of two or more above-described ionic polymers (see, e.g. Examples 9 and 10).
Particularly preferred anionic polymers include Eudragit® L100-55 (methacrylic acid and ethyl acrylate copolymer) and Eudragit® L100 or Eudragit® S100 (methacrylic acid and methyl methacrylate copolymers), all of which are available from Rohm America. Eudragit® L100-55 is soluble at a pH above 5.5 and practically insoluble at pH below 5.5. The molecular weight of Eudragit® L100-55 is approximately 250,000 D and the glass transition temperature is 110°C. Eudragit® L100 is soluble at pH above 6 and practically insoluble at pH below 6. The molecular weight of Eudragit® L100 is approximately 135,000 D and the glass transition temperature is about 150°C, Eudragit® S100 is soluble at pH above 7 and practically insoluble at pH below 7. The molecular weight of Eudragit® S100 is approximately 135,000 D and the glass transition temperature is about 160°C.
Particularly preferred cationic polymers include Eudragit® E (Rohm America), which is a copolymer of dimethylaminoethylmethacrylate and neutral methacrylic esters. This polymer is soluble up to pH 4 and is practically insoluble at a pH above 4. The molecular weight of Eudragit® E is approximately 150,000 D and the glass transition temperature is about 50°C.

Pharmaceutical compositions of the present invention Comprising the water-insoluble complexes of the invention may be manufactured in a manner that is known in the art, e.g. by means of conventional mixing, milling, encapsulating, dissolving, compressing, granulating, or lyophilizing processes. In addition to the water-insoluble complexes, these pharmaceutical compositions may also include therapeutically inert, inorganic or organic carriers ("pharmaceutically acceptable carriers"), other than the ionic polymer, and/or excipients. Pharmaceutically acceptable carriers for tablets, coated tablets, dragees and hard gelatin capsules include lactose, maize starch or derivatives thereof, talc, stearic acid or its salts. Suitable carriers for soft gelatin capsules include vegetable oils, waxes, fats, and semi-solid or liquid polyols.
The pharmaceutical compositions of the invention may also contain preserving agents, solubilizing agents, stabilizing agents, wetting agents, emulsifying agents, sweetening agents, coloring agents, flavoring agents, salts for varying the osmotic pressure, buffers, coating agents or antioxidants. These compositions may also contain additional therapeutically active compounds or more than one therapeutically active compound/polymer complex.
Methods of Preparation
In one embodiment of the present invention, water-insoluble complexes of the invention are prepared using one of the following methods:
a) Spray Drying or Lyophilizatlon Method: The therapeutically active compound and the ionic polymer are dissolved in a common solvent having a low boiling point, e.g., ethanol, methanol, acetone, etc. By means of spray drying or lyophilization, the solvent is evaporated, leaving the therapeutically active compound microprecipitated in amorphous form in the ionic polymer matrix. This technique is not preferable for those therapeutically active compounds that do not have adequate solubility (>5%) in the preferred solvents.

b) Solvent Controlled Precipitation: The therapeutically active compound and the ionic polymer are dissolved in a common solvent, e.g., dimethylacetamide, dimethylformamide, etc. The therapeutically active compound/ polymer solution is added to cold (2°-5°C) water adjusted to appropriate pH. The desired pH is dependent on the polymer used and is readily asertainablebly one skilled in the art. This causes the therapeutically active compound to microprecipitate in the polymer matrix. The microprecipitate is washed several times with aqueous medium until the residual solvent falls below an acceptable limit for that solvent. An "acceptable limit" for each solvent is determined pursuant to the International Conference on Harmonization (ICH) guidelines.
c) pH-Controlled Precipitation: In this process, microprecipitation of the therapeutically active compound in an ionic polymer is controlled by a drastic change in pH of the solution. The therapeutically active compound and the ionic polymer are dissolved at a high pH (e.g. pH ~ 9) and precipitated by lowering the pH of the solution (e.g. to - 1), or vice versa. This method is particularly suitable for therapeutically active compounds that have pH-dependent solubility.
d) Hot Melt Extrusion Process: Mircroprecipitation of a therapeutically active compound in an ionic polymer having thermoplastic characteristics can be achieved by a hot melt extrusion process. The crystalline therapeutically active compound and the polymer are mixed in a suitable blender and fed continuously to a temperature-controlled extruder causing the therapeutically active compound to be molecularly dispersed in the molten ionic polymer. The resulting extrudates are cooled to room temperature and milled into a fine powder.
e) Supercritical Fluid Technology: The therapeutically active compound and an ionic polymer are dissolved in a supercritical fluid such as liquid nitrogen or liquid carbon dioxide. The supercritical fluid is then removed by evaporation leaving the therapeutically active compound microprecipitated in the polymer matrix. In another method, the therapeutic compound and an ionic polymer is dissolved in a

suitable solvent. A microprecipitated powder can then be formed by spraying the solution in a supercritical fluid which acts as an antisolvent.
In another embodiment of the invention, pharmaceutical formulations may be prepared according to any one of the foregoing steps by the addition of a final step during which the compound/polymer complexes of the invention are formulated by methods well-known in the art.
In a preferred embodiment of the invention, the therapeutically active compound and the ionic polymer are dissolved in an organic solvent. Thereafter, the compound and the ionic polymer are co-precipitated relatively concurrently, preferably in aqueous solution, and preferably at a pH where, independently, neither the compound nor the polymer are soluble.
The organic solvent used to dissolve the therapeutically active compound and the ionic polymer should provide good solubility for both the poorly soluble compounds and the polymers used. These solvents include ethyl alcohol, methyl alcohol, acetone dimethyl sulfoxide, dimethyl acetamide, dimethyl formamide, N-methylpyrrolidone, Transcutol® (Diethylene glycol monoethyl ether, Gattefosse, Inc.),
glycofural, propylene carbonate, tetrahydrofuran, polyethylene glycols and propylene glycols.
The pH selected to co-precipitate the therapeutically active compound and the ionic polymer depends on the solubility of each of the specific polymers and compounds being precipitated. One skilled in the art can easily ascertain the preferred pH for co-precipitation for each combination of polymer and therapeutically active compound. In a preferred embodiment wherein an anionic polymer selected from Eudragit® L100-55, Eudragit® L100 and Eudragit® S100 is used, the solution is precipitated at a pH lower than about 4. In another preferred embodiment wherein the cationic polymer Eudragit® E100 is used, the solution is precipitated preferably at a pH above 4.

The amounts of therapeutically active compound(s) and polymer necessary to achieve the stable, water-insoluble complex of the invention may vary depending upon the particular compound and ionic polymer(s) used, as well as the particular solvent(s) and precipitation parameters. By way of example, the compound may be present in the complex from about 0.1% to about 80%, by weight. Analogously, the polymer is typically present in the complex in not less than about 20% by weight. Preferably, the compound is present in the complex from about 30% to about 70% by weight, more preferably from about 40% to about 60% by weight. Most preferably, the compound is present in the complex at about 50% by weight. For a complex incorporating Compound I, the compound is present in the complex at about 30-70% by weight, most preferably at about 50% by weight.
Once the compound/polymer complex precipitates out of solution, the resulting complex can be recovered from the solution by procedures known to those skilled in the art, for example by filtration, centrifugation, washing, etc. The recovered mass can then be dried (in air, an oven, or a vacuum) and the resulting solid can be milled, pulverized or micronized to a fine powder by means known in the art. The powder form of the complex can then be dispersed in a carrier to form a pharmaceutical preparation.
The pharmaceutical preparations according to the invention can be administered to a subject by any route suitable for achieving the desired therapeutic result(s). Preferred routes of administration include parenteral and oral administration.
The pharmaceutical formulations according to the invention include a therapeutically effective amount of a therapeutically active compound. A therapeutically effective amount means an amount, at such dosages and for such periods of time, necessary to achieve the desired therapeutic result. Moreover, such amount must be one in which the overall therapeutically beneficial effects outweigh

the toxic or undesirable side effects. A therapeutically effective amount of a
compound often varies according to disease state, age and weight of the subject
being treated. Thus, dosage regimens are typically adjusted to the individual
requirements in each particular case and are within the skill in the art.
By way of example, for Compound I above, the appropriate daily dose for administration to an adult human weighing about 70 kg is from about 10 mg to about 10,000 mg, preferably from about 200 mg to about 1,000 mg, although the upper limit may be exceeded when indicated.
The daily dosage of the therapeutically active compound can be administered as a single dose, in divided doses, or for parenteral administration, it may be given as subcutaneous injection.
The examples which follow refer to appended drawings wherein
FIG 1 is a powder x-ray diffraction pattern of the compound/polymer complex of Example 4 compared to the bulk drug alone and compared to drug and polymer physical mixture.
FIG. 2 is a powder x-ray diffraction pattern of samples from the compound/polymer complex of Example 4 exposed to accelerated stress conditions compared to unstressed (initial) compound/polymer complex.
FIG 3 is a plasma concentration profile in dogs of the compound/polymer complex of Example 4.
FIG 4 is a powder x-ray diffraction pattern for Compound II "as is" and as compound/polymer complex (Example 11) after microprecipitation in accordance with the invention.

FIG 5 is a powder x-ray diffraction pattern for Compound III "as is" and as compound/polymer complex (Example 13) after microprecipitation in accordance with the invention.
FIG 6 is a powder x-ray diffraction pattern for Compound IV "as is" and as compound/polymer complex (Example 15) after microprecipitation in accordance with the invention.
FIG 7 is a powder x-ray diffraction pattern for Compound V "as is" and as compound/polymer complex (Example 16) after microprecipitation in accordance with the invention.
EXAMPLES
The following examples illustrate methods for making the water-insoluble compound/polymer(s) complexes of the present invention as well as pharmaceutical preparations incorporating said complexes.
For the examples reported herein, the therapeutically active compounds tested were Compounds I, ll} III, IV and V, the structures for which are provided above. These compounds are practically insoluble in gastrointestinal fluids. Prior to the current invention, the crystalline, insoluble form of Compound I was the only stable form of this Compound that could be obtained.
General Procedures
Procedure Applicable to Example 1 (micronized compound)
Compound I was micronized using a fluid energy mill to yield an average particle size of 10 microns. This procedure did not alter the crystalline form of the compound.

Procedure Applicable to Example 2 (nanosized compound)
A 10% suspension of Compound I was wet milled in aqueous medium containing 5% Klucel EF® (Hydroxypropylcellulose, Aqualon Corp.) as a protective colloid to prevent aggregation. The milling was performed in batch mode in a Dynomill for 24 hours using 0.25 mm glass beads as milling media. The average particle size of the resultant suspension was 700 nm and the residue obtained after drying the suspension demonstrated that the compound was present in crystalline form,
Procedure Applicable to Example 3 (Pluronic F 68 dispersion)
A 10% dispersion of Compound I in 90% Pluronic F68 (polymer) was prepared using hot-melt technique. The compound was mixed into molten Pluronic F68 at 60°C
and the dispersion was then heated up to 180°C to dissolve Compound I. The solution was cooled to room temperature to yield a solid mass. The powder x-ray diffraction ("XRD") pattern of the molten dispersion was similar to that for Pluronic F68. This XRD shows that Compound I was thus present in the solid dispersion in amorphous form. The solid dispersion obtained by this technique was further dispersed in aqueous medium prior to use in dosing animals.
Procedure Applicable to Examples 4*12 and 15-16 (Molecular dispersion according to the invention)
In accordance with the method of the invention, compounds I, II, IV or V and the specific polymer identified in each instance (i.e., Eudragit® L100-55, Eudragit® L100 or Eudragit® S100) were dissolved in dimethylacetamide. The resulting solution
was then slowly added to cold (2-10°C) aqueous solution at pH 2 causing the compound and the polymer to co-precipitate as an insoluble matrix wherein the compound was molecularly dispersed in the polymer. In each case, the precipitate was washed several times with cold (2-10°C) aqueous solution at pH 2 until the residual dimethylacetamide was below 0.2%. The precipitate was dried in a forced air oven at 40°C for 24 hours to a moisture level of below 2% and milled using a Fitz

Mill (Fitzpatrick) at slow speed using forward knives and size 0 screen into desirable particle sizes. The desired mean particle size was 90% particles in the size range 50-400 jim.
Procedure applicable to Examples 13-14 (Compound III)
In accordance with the methods described above, Compound III and a specific polymer identified in each instance (i.e., Eudragit® L100-55, Eudragit® L100,
Hydroxypropylmethylcellulose phthalate (HP-50) or Eudragit® S100) were dissolved
in ethanol. The resulting solution was either dried in a vacuum oven at 40°C for 24 hours until the weight loss on drying was less than 2%, or alternatively, the solution was spray dried. As a result of this process, the compound and the polymer co-precipitated as an insoluble complex wherein the compound was molecularly dispersed in the polymer. The resulting dried film was ground with a pestle/mortar and screened through 60 mesh screen.
Data
Table 1 below summarizes the results of Examples 1-16. Table 1 specifies the individual therapeutically active compounds and, where applicable, the compound/polymer complex that was prepared, the method of preparing the compound/polymer complex, and the physical characteristics of the resulting products from each example.

As is shown in Figure 1 and Table 1, the powder x-ray diffraction (XRD) pattern of the complex resulting in Example 4, (Table 1), that is when Compound I is included in an ionic polymer in accordance with the process of the current invention, it takes an amorphous form.
Table I and Figures 4-7 also show that the methods of the present invention are useful in rendering Compounds II, III, IV and V in amorphous form.
The inclusion of Compound I in the ionic polymer protected the compound from external environmental effects such as moisture and heat. This result is demonstrated in Figure 2, wherein it is shown, by powder x-ray diffraction, that Compound I embedded in the polymer maintained its amorphous properties even under accelerated storage conditions. The ability of the complex to maintain Compound I in amorphous form even after storage at accelerated stress condition is due to the high molecular weight (> 80,000), high glass transition temperature (> 50°C) and insolubility in water of the polymer(s).
Furthermore, as is shown in Table 2 below, the bioavailability in dogs of Compound I when it is molecularly dispersed in an ionic polymer in accordance with the invention was unexpectedly higher than when the compound was administered to the animals in conventional forms (e.g. micronized and wet milled). Also shown in Table 2 are the bioavailability results obtained from solid dispersion of Compound I prepared by hot-melt method with Pluronic F68® (non-ionic water soluble polymer containing poly-oxyethyiene and polyoxypropylene chains, BASF). While the biovailability of the Compound in this solid dispersion was better than when the compound was micronized or in wet mill suspension, the physical stability of the solid dispersion was not satisfactory for a pharmaceutical product as is evident by the reversion of compound to its crystalline form within one month of storage at ambient conditions. The above-described results demonstrate the unsuitability for preparation of a pharmaceutical product of the solid dispersion technique in non-ionic water soluble polymers.

* Results are mean values (with standard deviations) for four animals (2 males and
2 females). ** Compared to Single Dose Intravenous Administration. *** Converts to crystalline form after exposure to 40°C, 75%RH, 1 wk, open
condition.
Figure 3 shows plasma concentration-time profile of different batches of the compound/polymer complex produced in accordance to Example 4. The results of these tests (summarized in Figure 3), show batch to batch reproducibility and consistency. Batch to batch reproducibility and consistency is an important aspect of any formulation that is intended for administration to human patients.

Figures 4-7 show that Compounds II, III, IV and V also can be converted into amorphous form using this invention.
In summary, as is shown by the data in Tables 1 and 2 above and in Figures 1, 2, and 4-7, the powder x-ray diffraction patterns of the compound/polymer(s) complexes obtained in Examples 4-16 shows that molecularly dispersing a poorly soluble compound in an ionic polymer(s) according to the present invention converts the compounds into amorphous form and maintains excellent stability of the amorphous compound upon long-term storage.

CLAIMS
1. A pharmaceutical composition comprising a water-insoluble complex of a therapeutically active, stable amorphous compound and a water-insoluble ionic polymer that has a molecular weight greater than about 80,000 D and a glass transition temperature equal to or greater than about 50°C, and a carrier.
2. The pharmaceutical composition of claim 1 wherein the therapeutically active compound is a compound that is poorly soluble when in crystalline form.
3. The pharmaceutical composition of claim 2 wherein the poorly soluble therapeutically active compound in its crystalline form has a solubility of less than 1 mg/mL in aqueous solution,
4. The pharmaceutical composition of claim 1 wherein the ionic polymer is a cationic polymer.
5. The pharmaceutical composition of claim 4 wherein the cationic polymer is a copolymer of dimethylaminoethylmethacrylate and neutral methacrylic ester.
6. The pharmaceutical composition of claim 5 wherein the cationic polymer is Eudragit E®.
7. The pharmaceutical composition of claim 1 wherein the ionic polymer is an anionic polymer.
8. The pharmaceutical composition of claim 7 wherein the anionic polymer is a copolymer of methacrylic acid and ethyl acrylate or methacrylic acid and methyl methacrylate.

9. The pharmaceutical composition of claim 8 wherein the anionic polymer
is selected from the group consisting of Eudragit L 100-55® , Eudragit MOO® and
Eudragit S-100®.
10. The pharmaceutical composition of claim 7 wherein the anionic polymer is selected from the group consisting of polyvinyl acetate phthalate, cellulose acetate phthalate, hydroxypropylmethylcellulose phthalate, cellulose acetate terphthalate, polycyanoacrylate and hydroxypropyl methyl cellulose acetyl succinate, carboxy methyl cellulose, and low substituted hydroxypropylcellulose.
11. The pharmaceutical composition of claim 1 wherein the solubility of the ionic polymer is pH dependent.
12. The pharmaceutical composition of claim 11 wherein the ionic polymer is insoluble at pH above about 4.
13. The pharmaceutical composition of claim 1 wherein the ionic polymer and the therapeutically active compound in its crystalline form are both relatively insoluble above pH of about 4.
14. The pharmaceutical composition of claim 11 wherein the ionic polymer is insoluble at pH below about 4.
15. The pharmaceutical composition of claim 1 wherein the ionic polymer and the therapeutically active compound in its crystalline form are both relatively insoluble at pH below about 4.
16. The pharmaceutical composition of claim 1 wherein the therapeutically active compound is selected from the group consisting of Compounds I, II, III, IV, V, VI, VII and VIII.

17. A pharmaceutical composition comprising a water-insoluble complex of Compound I in stable amorphous form and a water-insoluble ionic polymer that has a molecular weight greater than about 80,000 D and a glass transition temperature equal to or greater than about 50°C, and a carrier.
18. The pharmaceutical composition of claim 1 wherein the ionic polymer is present in the water-insoluble complex at not less than about 20%, by weight.
19. The pharmaceutical composition of claim 18 wherein the therapeutically active compound is present in the water-insoluble complex at about 0.1% to about 80%, by weight of said complex,
20. The pharmaceutical composition of claim 19 wherein the therapeutically active compound is present in the water-insoluble complex at about 30% to about 70%, by weight of said complex.
21. The pharmaceutical composition of claim 20 wherein the ionic polymer is present in the water-insoluble complex at about 50%, by weight, and the therapeutically active compound is present at about 50%, by weight of said complex.
22. A method for preparing a pharmaceutical formulation comprising a water-insoluble complex of a stable, amorphous therapeutically active compound and an ionic polymer comprising:

(a) dissolving the therapeutically active compound and ionic polymer in a suitable solvent;
(b) contacting the solution of step (a) with an aqueous solution at a pH in which the ionic polymer is poorly soluble thereby microprecipitation the therapeutically active compound and ionic polymer as a compound/polymer complex;
(c) preparing a pharmaceutical formulation that includes the compound/ polymer complex of step (b) above.

23. The method of claim 22, wherein in step (a), the therapeutically active compound and the ionic polymer are dissolved in a solvent selected from the group consisting of ethyl alcohol, methyl alcohol, dimethylsulfoxide, dimethylacetamide, dimethyl formamide, N-methylpyrrolidone, Transcutol® (diethylene glycol monoethyl ether, Gattefosse), glycofural, propylene carbonate, tetrahydrofuran, polyethylene glycol and propylene glycol,
24. The method in claim 22 wherein in step (b), microprecipitation is carried out by removing the solvent by spray drying or lyophilizing.
25. The method of claim 22 wherein in step (a), the insoluble therapeutically active and the ionic compound polymer are dissolved by adjusting the pH,
26. The method of claim 22 wherein after step (b), residual solvent is removed.
27. The method of claim 26 wherein the residual solvent is removed by washing the compound/polymer complex,
28. The method of claim 26 wherein the residual solvent is removed by evaporation or drying.
29. The method of claim 28 wherein the residual solvent is removed by spray drying.
30. A method for preparing a pharmaceutical formulation comprising a water-insoluble complex of a stable, amorphous therapeutically active compound and an ionic polymer comprising:
(a) dissolving the therapeutically active compound, in its crystalline form, and ionic polymer in an organic solvent;

(b) contacting the product of step (a) with an aqueous solution at a pH at which the ionic polymer and the .therapeutically active compound will precipitate as a compound/polymer matrix;
(c) washing the compound/polymer matrix;
(d) drying the compound/polymer matrix; and
(e) preparing a pharmaceutical formulation that incorporates the washed and dried compound/polymer matrix of step (d) above.

31. The method of claim 30 wherein the therapeutically active compound that is incorporated in the compound/polymer matrix is predominantly in amorphous form.
32. The method of claim 31 wherein the ionic polymer is selected from the group consisting of Eudragit® E100, Eudragit® L100, Eudragit® L100-55 and Eudragit® S100.
33. A method for preparing a water-insoluble complex of a stable amorphous compound and an ionic polymer comprising:

(a) melting together the therapeutically active compound and the ionic polymer; and
(b) cooling the mixture resulting from step (a).
34. A method for preparing a pharmaceutical formulation comprising a
water-insoluble complex of a stable amorphous compound and an ionic polymer
comprising:
(a) dissolving the therapeutically active compound and the ionic polymer in a supercritical fluid;
(b) removing the supercritical fluid resulting in the microprecipitation of the therapeutically active compound in the polymer matrix; and
(c) preparing a pharmaceutical^ formulation that includes the product of step (b) above.

35. The method of claim 34 wherein the supercritical fluid used in step (a) is selected from the group consisting of liquid nitrogen and liquid carbon dioxide.
36. The method of claim 34 wherein removal of the supercritical fluid in step (b) is accomplished by evaporation.
37. The method of claim 22, 30, 33, 34 or 35 wherein the therapeutically active compound is selected from the group consisting of Compounds I, II, III, IV, V, VI, VII and VIII,
38. A stable, water-insoluble complex prepared by

(a) dissolving Compound I and a water-insoluble ionic polymer having a molecular weight greater than 80,000D and a glass transition temperature equal to or greater than 50°C in a suitable solvent; and
(b) co-precipitating Compound I and the ionic polymer as a compound/polymer complex.

39. The complex of claim 38 wherein precipitation in step (b) in effected by contacting the solution of step (a) with an aqueous solution at a pH in which the ionic polymer is poorly soluble.
40. A water-insoluble complex comprising a stable, amorphous compound and a water-insoluble ionic polymer that has a molecular weight greater than 80,000 D and a glass transition temperature equal to or greater than 50°C.
41. The complex of claim 40 wherein the amorphous compound is poorly soluble in crystalline form.
42. A water-insoluble complex comprising Compound I in stable amorphous form and a water-insoluble ionic polymer that has a molecular weight

greater than 80,000 D and a glass transition temperature equal to or greater than 50°C.
43, A method for stabilizing an amorphous compound comprising
molecularly dispersing the compound in a water-insoluble ionic polymer that has a
molecular weight greater than 80,000 D and a glass transition temperature equal to
or greater than 50°C.
44. A method for converting a poorly soluble, crystalline compound to a
stable amorphous form of said compound comprising molecularly dispersing said
compound in a water-insoluble ionic polymer that has a molecular weight greater than
80,000 D and a glass transition temperature equal to or greater than 50°C.
45, A therapeutically active compound in stable amorphous form
molecularly dispersed in a water-insoluble ionic polymer that has a molecular weight
greater than 80,000 D and a glass transition temperature equal to or greater than
50°C.
46. The invention as herein before described.

Documents:

929-mas-1999-abstract.pdf

929-mas-1999-claims filed.pdf

929-mas-1999-claims granted.pdf

929-mas-1999-correspondence others.pdf

929-mas-1999-correspondence po.pdf

929-mas-1999-description complete filed.pdf

929-mas-1999-description complete granted.pdf

929-mas-1999-drawings.pdf

929-mas-1999-form 1.pdf

929-mas-1999-form 18.pdf

929-mas-1999-form 26.pdf

929-mas-1999-form 3.pdf

929-mas-1999-form 5.pdf

929-mas-1999-other documents.pdf

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Patent Number

211561

Indian Patent Application Number

929/MAS/1999

PG Journal Number

50/2007

Publication Date

14-Dec-2007

Grant Date

05-Nov-2007

Date of Filing

20-Sep-1999

Name of Patentee

M/S. F. HOFFMANN-LA ROCHE AG

Applicant Address

124 GRENZACHERSTRASSE, CH-4070 BASEL,

Inventors:

#	Inventor's Name	Inventor's Address
1	ANTONIA A ALBANO	58 STARMOND AVENUE, CLIFTON, NEW JERSEY 07013,
2	WANTANEE PHUAPRADIT	42 ROBINSON TERRACE, CLIFTON, NEW JERSEY 07013,
3	HARPREET K SANDHU	73 MANGER ROAD, WEST ORANGE, NEW JERSEY 07052,
4	NAVNIT HARGOVINDAS SHAH	203 BEVERLY HILL ROAD, CLIFTON, NEW JERSEY 07012,

PCT International Classification Number

A61K 31/404

PCT International Application Number

N/A

PCT International Filing date

PCT Conventions:

#	PCT Application Number	Date of Convention	Priority Country
1	60/101, 336	1998-09-22	U.S.A.
2	60/136, 531	1999-05-28	U.S.A.