Title of Invention

PROCESS FOR THE BIOLOGICAL PRODUCTION OF 1,3-PROPANEDIOL WITH HIGH TITER

Abstract

The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of an E. coli transformed with the Klebsiella pneumoniae dha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type Klebsiella pneumoniae. In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT). The dramatically improved process relies on the presence in E. Coli of a gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol.

Full Text

Documents:

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in-pct-2002-00040-mum-claims(granted)-(22-11-2005).doc

in-pct-2002-00040-mum-claims(granted)-(22-11-2005).pdf

IN-PCT-2002-00040-MUM-CORRESPONDENCE(2-4-2012).pdf

in-pct-2002-00040-mum-correspondence(26-07-2005).pdf

in-pct-2002-00040-mum-correspondence(ipo)-(16-11-2005).pdf

in-pct-2002-00040-mum-drawing(22-11-2005).pdf

in-pct-2002-00040-mum-form 1(11-01-2002).pdf

in-pct-2002-00040-mum-form 19(18-08-2004).pdf

in-pct-2002-00040-mum-form 1a(26-07-2005).pdf

in-pct-2002-00040-mum-form 2(granted)-(22-11-2005).doc

in-pct-2002-00040-mum-form 2(granted)-(22-11-2005).pdf

in-pct-2002-00040-mum-form 3(11-01-2002).pdf

in-pct-2002-00040-mum-form 3(26-07-2005).pdf

in-pct-2002-00040-mum-form 5(11-01-2002).pdf

in-pct-2002-00040-mum-form-pct-ipea-409(11-01-2002).pdf

in-pct-2002-00040-mum-form-pct-isa-210(11-01-2002).pdf

in-pct-2002-00040-mum-other pct(22-11-2005).pdf

in-pct-2002-00040-mum-petition under rule 137(26-07-2005).pdf

in-pct-2002-00040-mum-petition under rule 138(26-07-2005).pdf

IN-PCT-2002-00040-MUM-PETITION UNDER RULE-138(2-4-2012).pdf

in-pct-2002-00040-mum-power of authority(11-01-2002).pdf

in-pct-2002-00040-mum-power of authority(11-02-2002).pdf

in-pct-2002-00040-mum-power of authority(26-07-2005).pdf