Indian Patents. 212241:N-BIPHENYLMETHYL AMINOCYCLOALKANECARBOXAMIDE DERIVATIVES WITH A SUBSTITUENT ON THE METHYL USEFUL AS BRADYKININ ANTAGONISTS

Title of Invention	N-BIPHENYLMETHYL AMINOCYCLOALKANECARBOXAMIDE DERIVATIVES WITH A SUBSTITUENT ON THE METHYL USEFUL AS BRADYKININ ANTAGONISTS
Abstract	N-Biphenyl(substituted methyl)aminocycloalkanecarboxamide derivatives are bradykinin B1 antagonists or inverse agonists useful in the treatment or prevention of symptoms such as pain and inflammation associated with the bradykinin B1 pathway.

Full Text	N-BIPKENYLMETHYL AMINOCYCLOALKANECARBOXAMIDE DERIVATES VIITH A SU3STITUENT ON THE METHYL USEFUL AS BRADYKIMIN A^TTAGONISTS BACKGROUND OF THE INVENTION This invention is directed to aminocycloalkanecarboxamide compounds. In particular, this invention is directed to aniinocycloalkanecarboxamide compounds that are bradykinin antagonists or inverse agonists. Bradyldnin ("BK") is a kinin which plays an important role in the pathophysiological processes accompanying acute and chronic pain and inflammation. Bradykinin (BK), like other kinins, is an autacoid peptide produced by the catalytic action of kallikrein enzymes on plasma and tissue precursors termed kininogens. The biological actions of BK are mediated by at least two major G-protein-coupled BK receptors termed Bl and B2. It is generally believed that B2 receptors, but not Bl receptors, are expressed in normal tissues and that inflammation, tissue damage or bacterial infection can rapidly induce Bl receptor expression. This makes the Bl receptor a particularly attractive drug target. The putative role of kinins, and specifically BK, in the managem.ent of pain and inflammation has provided the impetus for developing potent and selective BK antagonists. In recent years, this effort has been heightened with the expectation that useful therapeutic agents with analgesic and anti-inflammatory properties would provide relief from maladies mediated through a BK receptor pathway (see e.g., M.G. Bock and J, Longmore, Current Opinion in Chem. Biol., 4:401-406(2000)). Accordingly, there is a need for novel compounds that are effective in blocking or reversing activation of bradykinin receptors. Such compounds would be useful in the management of pain and inflammation, as well as in the treatment or prevention of diseases and disorders mediated by bradykinin; further, such compounds are also useful as research tools {in vivo and in vitro), Canadian Published Application No. 2,050,769 discloses compounds of the formula: which are intermediates in the preparation of angiotensin II antagonists. SUMMARY OF THE INVENTION The present invention provides biphenyl cycloalkanecarboxamide derivatives which are bradykir.in antagonists or inverse agonists, pharmaceutical compositions containing such compounds, and methods of using them as therapeutic agents. DET.AILED DESCRIPTION OF THE INVENTION The present invention provides compounds of formula I and pharmaceutically acceptable salts thereof: wherein Rl and R2 are independently selected from (1) hydrogen and (2) C1-4alkyl; R3a is selected from (1) hydrogen and (2) C1-4 alkyl optionally substituted with 1 to 5 halogen atoms; R3b is C1-4 alkyl optionally substituted with 1 to 5 halogen atoms; R4a and R4b are independently selected form (1) hydrogen. (2) halogen, and (3) C1-4 alkyl optionally substituted with 1 to 4 groups selected from halogen, ORa, OC(0)Ra. S(0)kRd, OS(0)2Rd, and NRIR2, or R4a and R4b together with the carbon atom to which they are both attached form an exo-cyclic methylene optionaly substituted with 1 to 2 groups selected from C1-4 alkyl optionally substituted wi:n 1-5 halogens and C1-4 alkyloxy: R5 is selected from (1) C1-6 alkyl optionally substituted with 1 to 5 groups independently selected from halogen, nitro, cyano, ORa, SRa, CORa, S02Rd, C02Ra. OC(0)Ra, NRbRc, NRbC(0)Ra, NRbC(0)2Ra, C(0)NRbRC, C3-8 cycloalkyl, (2) C3-8 cyc'-oalkyl optionally substituted with 1 to 5 groups independently selected from halogen, nitro, cyano and phenyl, (3) C3-6 alkynyl, (4) C2-6 alkenyl optionally substituted with hydroxyethyl, (5) (CH2)k-aryl optionally substituted with 1 to 3 groups independently selected from halogen, nitro, cyano, ORa, SRa, C(0)2Ra, C1-4 alkyl and C1-3 haloalkyl, wherein aryl is selected from phenyl, 3,4-methyienedioxyphenyI and naphthyl; (6) (CH2)k-beterocycle optionally substituted with 1 to 3 groups independently selected from halogen, nitro, cyano, ORa, SRa, C1-4 alkyl and Ci_3 haloalkyl wherein said heterocycle is selected from (a) a 5-membered heteroaromatic ring having a ring heteroatom selected from N, O and S, and optionally having up to 3 addidonal ring nitrogen atoms wherein said ring is optionally benzo-fused; (b) a 6-membered heteroaromatic ring containing from 1 to 3 ring nitrogen atoms and N-oxides thereof, wherein said ring is optionally benzo-fused; and (c) a 5- or 6-membered non-aromatic heterocyclic ring selected from tetrahydrofuranyl, "5-oxo-tetrahydrofuranyl, 2-oxo-2H-pyranyl, 6-oxo-l,6-dihydropyridazinyI, (7) C(0)2Ra, and (8) C(0)NRbRC; R6a is selected from (1) C1-8 alkyl optionally substituted with 1-5 groups independently selected from halogen, nitro, cyano, CORa, C02Ra, C(0)NRbRc, ORa, OC(0)Ra, SRa, S02Rd, S(0)Rd, NRbRc, N-Rbc(0)Ra, NRbS02R^, NRbc02Ra, (2) C3-8 cycloalkyl. (3) C2-8 alkenyl optionally substituted with C02Ra, (4) halogen, (5) cyano, (6) nitro, (7) NRbRc, (8) NRbC(0)Ra, (9) NRbC02Ra, (10) NRbc(0)NRbRc, (11) NRbC(0)NRbC02Ra (12) NRbS02Rd, (13) CC)2Ra, (14) CORa, (15) C(0)NRbRc, (16) C(0)NHORa, (17) C(=NORa)Ra (18) C(=NORa)NRbRc, (19) ORa (20) OC(0)Ra, (21) S(0)kRd, (22) S02iNfRbRc, and (23) optionally substituted heterocycle where the heterocycle is a 5- membered heteroaromatic ring having a ring heteroatom selected from N, O and S, and optionally having up to 3 additional ring nitrogen atonis, 4,5-dihydro-oxazolyl and 4,5-dihydro-l,2,4-oxadiazoIyl, and wherein said substituent is 1 to 3 groups independently selected from C1-4 alkyl optionally substituted with 1 to 5 Kalogen atoms, ORa or OC(0)Ra. R6b and R6C are independently selected from (1) hydrogen, and (2) a group from R6a; with the proviso that not more than one of R6a, R6b, and R6C is a heterocycle; R7a and R7b are independently s-elected from (1) hydrogen, (2) halogen, (3) cyano. (4) nitro, (5) ORa, (6) C02Ra (7) C(0)NRbRc, (8) C1-4 alky! optionally substituted with i to 5 halogen atoms, (9) NRbRc, and (10) S(0)kRd; Ra is selected from (1) hydrogen, (2) C1-4 alky! optionally substituted with i to 5 halogen atoms, (3) phenyl optionally substituted with 1 to 3 groups independently selected from halogen, cyano, nitro, OH, C1-4 alkyloxy, C3-6 cycloalkyl and C1-4 alkyl optionally substituted with 1 to 5 halogen atoms, (4) C3-6 cycloalkyl, and (5) pyridyl optionally substituted with 1 to 3 groups independently selected from halogen and C1-4 alkyl; Rb and Rc are independently selected from (1) hydrogen, (2) C1-4 alkyl optionally substituted with I to 5 groups independently selected from halogen , amino, mono-C1-4alkylamino, di-C1-4alkylamino, and S02Rd, (3) (CH2)k-phenyl optionally substituted with 1 to 3 groups selected from halogen, cyano, nitro, OH, C1-4 alkyloxy. C3.6 cycloalkyl and C1-4 alkyl optionally substituted with 1 to 5 halogen atoms, and (4) C3.6 cycloalkyl, or Rb and Rc together with the nitrogen atom to which they are attached form a4-, 5-, or 6-membered ring optionally containing an additional heteroatom selected from N, O, and S; or Rb and Rc together with the nitrogen atom to which they are attached form a cyclic imide; Rd is selected from (1) C1-4 alkyl optionally substituted with 1 to 5 halogen atoms, (2) C1-4 alkyloxy, and (3) phenyl optionally substituted with 1 to 3 groups selected from halogen, cyano, nitro, OH, C1-4 alkyloxy, C3-6 cycloalkyl and C1-4 alkyl optionally substituted with I to 5 halogen atoms; k is 0,1 or 2; and misO or 1. For compounds of fonnula I, examples of Rl and R2 include hydrogen, methyl, ethyl, n-propyl, isopropyl, n-butyl, t-butyl, isobutyl and sec-butyl. In one embodiment of formula I are compounds wherein R1 and R2 are each hydrogen. Examples of R3a and R3b for compounds of formula I include hydrogen, methyl, ethyl, n-propyl, isopropyl, n-butyl, t-butyl, isobutyl, sec-butyl, chloromethyl, fluromethyl, trifluoromethyl, 2,2,2-trifluoroethyl, 2,2-difluoroethyl, 1,1,2,2.2-pentafluoroethyl, and the like. In one embodiment of formula I are compounds wherein one of R3a is hydrogen and R3b is C1.4 alkyl. In one subset thereof R3b is methyl. Examples R4a and R4b for compounds of formula I include hydrogen, methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, isobutyl, t-butyl, chlorine, fluorine, bromine, chloromethyl, 1-chloroethyl, hydroxymethyl, 2-methoxyethyl, ethoxymethyl, acetyloxymethyl, methylthiomethyl, aminomethyl, methylamino-methyl, (dimethylamino)methyl, (methylsulfonyl)oxyraethyl, and the like; or R4a and R4b on the same carbon atom taken together represent methylene. In one embodiment of formula I are compounds wherein one of R4a and R4b is hydrogen and the other is selected from hydrogen, halogen and C1-4 alkyl optionally substituted with a group selected from halogen, ORa, OC(0)Ra, S(0)kRd, OS(0)2Rd, and NRiR2, or R4a and R4b together with the carbon atom to which they are both-attached fonn an exo-cyclic methylene. In one subset thereof R4a and R4b are each hydrogen; in another subset R4a is hydrogen and R^b is selected from CH2-halogen, CH2-0Ra, CH2-0C(0)Ra CH2-S(0)kRd CH2-OS(0)2Rd and CH2-NR1R2; in a further subset R4a is hydrogen and R4b is selected from hydroxymethyl, acetyloxymethyl, chloromethyl, (methanesulfonyl)oxymethyl, (methylthio)methyl and (dimethylamino)methyl. Examples of R5 for compounds of formula I include methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyL isobutyl, t-butyl, 1-ethylpropyl, 2,2-dimethyl-propyl, broraomethyl, chloromethyl, dichloromethyl, difluoromethyl, trifluoromethyl, chlorodifluoromethyl, cyanomethyl, aminomethyl, acetylaminomethyl, dimethyl- aminomethyl, hydroxymethyl, methoxymethyl, ethoxymethyl, methylsulfonylmethyl, phenylthiomethyl, phenoxymethyl, 1-aminoethyl, 1-acetylaminomethyl, 1-imidazolyl- methyl, t-butoxycarbonylaminomethyl, 3-pyridylcarbonylmethyl, 1-chloroethyl, 1,1- dichloroethyl, 2,2,2-trifluoroethyI, pentafluoroethyl, 2-methoxyethyl, 2-phenylethy!, 2-cyclopentylethyl, 2-carboxyethyl, 2-methoxy-2-oxoethyl, 2-nitroethyI^ 1,1-difluoro- i-hydroxypropyl, 1-hydroxypropyI, 2-oxopropyl, 3-metho\y-3-oxopropyl, 1-cyano- cyclopropyl, cyclopropyh cyclopentyl, 2-phenylcyclopropy!, allyl, ethenyl, 1-(1- hydroxyethyi)vinyl, 3-burynyl, propargyl, phenyl, benzyl, 3,5-bis(trifluoromethyl)- phenyl, 2,4-difluordphenyl, 4-nneihylphenyl, 3,4-dimethoxybenzyl, 3,4-dimethoxy- phenyl, 4-cyanophenyl, 3-nitrophenyl, 2-naphthyl, 3,4-methyIenedioxyphenyl, 3- cyanophenyl, 2-cyanophenyl, 3-fiuorophenyl, 3-methoxyphenyl, 3-chIorophenyl, 3,4- dichlorophenyl, 3,5-dimethoxyphenyl, 3-trifluoromethylphenyl, 3-methylphenyl, 3,5- dichlorophenyl, 2-hydroxyphenyl, 3-hydroxyphenyl, 3'nitro-5-(trifluoromethyl)- phenyl, 5-isoxazolyI, 2-benzothienyl, 2-thienylmethyU 3-pyridyl, 4-pyridyl, 2-furyl, 3- furyl, 2-thienyl, 3-thienyI, 5-methyl-3-isoxazolyl, 3-tetrahydrofuranyl, 4-methyl-l,2,5- oxadiazol-3-yl, 5-carboxy-3-pyridyI, 6-hydroxy-2-pyridyl, 5-hydroxy-3-pyridyl, 2- hydroxy-3-pyridyU 2-methyoxy-3-pyridyl, 6-chloro-2-pyridyl, 2-chloro-3-pyridyl, 5- chloro-3-pyridyl, 5-fIuoro-3-pyridyl, 5-bromo-3-pyridyl, 5-methyl-3-pyridyl, 3- (trifluoromethyl)-4-pyridyl, 5-(tririuoromethy!)-3-pyridyil, l-methyl-4-pyrazoIyl, 1- pyrazolylmethyl, l-methyl-2-imidazolyl, l,2,4-triazol-l-ylmethyl,4-thiazo!yl, 5-oxo- tetrahydrofuran-2-yl, 2-oxo-5-pyranyl, 3-isoxazolyl, 3-pyridazinyl, 5-pyrimidinyl, 4- pyrimidinyl, l-methyl-5-pyrazoIylm l-methyl-3-pyrazolyl, 5-thiazolyl, 5-methyl-1- pyrazoIyImethyI/(3-methyl-l,2,4-toa2ol-5-yl)methyl, 2-(l,2,4-triazol-l-yl)rthyl,5- raethyM-thiazolyl, 2-quinoxaIinyl, methoxycarbonyl, aminocarbonyl, methylaraino- carbonyl, dimethylaminocarbonyl, 2-(dimethylamino)ethyIaminocarbonyl, benzyl- aminocarbonyl, 2-phenethylaniinocarbonyL In one embodiment of fonnula I are compounds wherein R5 is C1-6 alkyl optionally substituted with 1 to 5 groups independently selected from halogen, nitre, cyano, ORa, SRa, CORa, S02Rd, C02Ra OC(0)Ra NRbRc, NRbC(0)Ra, NRbC02Ra, C(0)NRbRc, and C3-8 cycloalkyl. In one subset thereof are compounds wherein R5 is C1-5 alkyl optionally substituted with 1 to 5 groups independently selected from halogen, nitro, cyano, ORa, SRa, C02Ra and C3-8 cycloalkyl. In a further subset are compounds wherein R5 is selected from C1.5 alkyl and C1-3 alkyl substituted with 1 to 5 groups selected from halogen, cyano, hydroxy, C1-4 alkoxy and C1-4 alkoxycarbonyl. In another further subset R5 is selected from C1-3 alkyl substituted with 1 to 5 halogen atoms, or a group selected from cyano, hydroxy, C1-4 alkoxy and C1-4 alkoxycarbonyl. In another embodiment of formula I are compounds wherein R5 is C3-6 cycloalkyl optionally substituted with 1 to 3 groups independently selected from halogen, nitro, cyano and phenyl. In one subset R5 is C3-6 cycloalkyl optionally substituted with a group selected from cyano and phenyl. In another embodiment of formula I are compounds wherein R5 is (CH2)k-aryl optionally substituted with 1 to 3 groups independently selected from halogen, nitro, cyano, ORa, SRa, C1-4 alkyl and C1-3 haloalkyl, wherein aryl is selected from phenyl, 3,4-methylenedioxyphenyl and naphthyl. In one subset thereof, R5 is phenyl optionally substituted with 1 to 3 groups independently selected from halogen, trifluoromethyl, nitro, cyano, C1-4 alkoxy and C1-4 alkyl; in a further subset R5 is phenyl optionally substituted with 1 to 2 groups selected from methyl, trifluoromethyl, halogen, cyano, nitro and methoxy. In another embodiment of formula I are compounds wherein R5 is (CH2)k-heterocycle optionally substituted with 1 to 2 groups independently selected from halogen, nitro, cyano, ORa, SRa, C1-4 alkyl and C1-3 haloalkyl wherein said heterocycle is selected from isoxazolyl, thienyl, pyridinyl, benzothienyl, furyl, tetrahydrofuranyl, oxadiazolyl, I-oxidopyridinyl, pyrazolyl, imidazolyl, 1,2,4-triazolyl, thiazolyl, 5-oxotetrahydrofuranyl, 2-oxo-2H-pyranyl, 6-0x0-1,6-dihydro- pyridazinyl, oxazolyl, pyridazinyl. pyrimidinyl and quinoxalinyl In one subset thereof R5 is selected from isoxazolyl optionally substituted with 1 or 2 Ci^ alkyl, thienyl, pyridinyl optionally substituted with hydroxy, trifluoromethyl or halogen, benzothienyl, furyl, tetrahydrofuranyl, oxadiazolyl optionally substituted with C1-4 alkyl, 1-oxidopyridinyl optionally substituted with halogen or C1-4 alkyl, pyrazolyl opdonally substituted with C1-4 alkyl, irnidazolyl optionally substituted with C1-4 alkyl, 1,2,4-triazolyl optionally substituted with C1-4 alkyl, thiazolyl optionally substituted with C1-4 alkyl, 5-oxotetrahydrofuranyl, 2-oxo-2H-pyranyl, 6-oxo-l,6- dihydropyridazinyl, oxazolyl, pyridazinyl, pyrimidinyl and quinoxalinyl. In another subset R5 is selected from 5-isoxazolyl, 5-pyrimidinyl, 5-bromo-3-pyridyl and N-oxide thereof, and 5-trifluoromethyl-3-pyridyl For compounds of formula I examples of R6a include 1-methylethyI, 1-hydroxyethyl, methoxymethyl, 2-oxo-2-methoxyethyl, carboxy, methoxycarbonyl, ethoxycarbonyl, isopropoxycarbonyl, phenoxycarbonyl, cyclopentoxycarbonyl, cyclo- butoxycarbonyl, cyclopropoxycarbonyl, 2,2,2-trifluoroethoxycarbonyl, 4-trifIuoro-methylphenoxycarbonyl, methoxyaminocarbonyl, methoxycarbonylmethyl, formyl, hydroxy, 3-methyl-l,2,4-oxadiazol-5-yl, 5-methyl-l,2,4-oxadiazol-3-yl, l-methyl-5-tetrazolyl, 2-methyl-5-tetrazoly!, cyano, hydroxy, methoxy, difluoromethoxy, trifluoromethoxy, trifluoromethyl, chloro, fluoro, methylaminosulfonyl, dimethyl-aminosulfonyl, methoxycarbonylamino, ethoxycarbonylamino, 2-fluoroethoxy-carbonylamino, isopropoxycarbonylamino, methylaminocarbonylamino, dimethyl-amino, methylaminocarbonyl, isopropylaminocarbonyl, ethylaminocarbonyl, cyclo-propylaminocarbonyl, cyclobutylaminocarbonyl, dimethylaminocarbonyl and amino-carbonyl; examples for R6b for compounds of formula I include hydrogen, chloro, fluoro, methyl and methoxycarbonyl; example of R6C include hydrogen, chloro, fluoro and methyl; and examples of R^a and R7b include hydrogen, hydroxy, methoxy, methylamino, methylsulfonyl, chloro and fluoro. In another embodiment of formula I are compounds wherein m is 0. In another embodiment of formula I are compounds represented by formula 1(1): wherein m, Rl, R2, R3a R3b, R4a, R4b, R5, R6a, R6b, R6C and R7a have the same definitions as provided under formula L In a subset of formula 1(1) are compounds wherein R6a is selected from (1) C02Ra (2) C(0)NHORa, (3) cyano, (4) halogen, (5) ORa, (6) C1-8 alkyl optionally substituted with 1-5 halogen atoms, or a group selected from COaRa, C(0)NRbRc and ORa, (7) C(0)NRbRc, (8) NRbC(0)NRbRc, (9) NRbc(0)ORa, and (10) optionally substituted heterocycle where the heterocycle is selected from oxadiazolyl and tetrazolyl and wherein said substituent is 1 to 3 groups independently selected from C1-4 alkyl optionally substituted with 1 to 5 halogen atoms, ORa or OC(0)Ra. In a further subset are compounds wherein R6a is selected from C02R^, C(0)NHORa, methyltetrazolyl, methyloxadiazolyl, NRbC(0)NRbRc, and NRbC(0)ORa In another subset of formula 1(1) are compounds wherein R6b is selected from hydrogen, halogen and C02Ra. In a further subset R6b is hydrogen or halogen. In another subset of formula 1(1) are compounds where R6a is selected from (1) C02Ra, (2) C(0)NHORa, (3) cyano, (4) halogen, (5) ORa, (6) C1-8 alkyl optionally substituted with 1-5 halogen atoms, or a group selected from C02Ra, C(0)NRbRc and ORa, (7) C(0)NRbRc, (8) NRbC(0)NRbRc, (p) NRbc(0)ORa, and (10) optionally substituted heterocycle where the heterocycle is selected from oxadiazolyl and tetrazolyl and wherein said substituent is 1 to 3 groups independently selected from C1-4 alkyl optionally substituted with 1 to 5 halogen atoms, ORa or OC(0)Ra; R6b is selected from hydrogen and halogen; and R6C is hydrogen. In another subset of formula 1(1) are compounds wherein R5 is selected from C1-4 alkyl optionally substituted with 1 to 5 halogen atoms or a cyano group, C3-6 cycloalkyl, isoxazolyl, pyrimidinyl and pyridinyl (and N-oxide thereof) optionally substituted with halogen. 4 In one embodiment of formula 1(2), R3b is methyl In another embodiment of formula 1(2), R6b is hydrogen or halogen. In one subset R6b is hydrogen; in another subset R^b is fluorine or chlorine. In another embodiment of formula 1(2), R6a is selected from (1) C02Ra, (2) C(0)NHORa, (3) cyano, (4) halogen, (5) ORa, (6) C1-8 alkyl optionally substituted with 1-5 halogen atoms, or a group selected from C02Ra, C(0)NRbRc and ORa, (7) C(0)NRbRc, (8) XRbc(0)NRbRc, (9) NRbC(0)0Ra, and (10) optionally substituted heterocycle where the heterocycle is selected from oxadiazolyl and tetrazolyl and wherein said substituent is 1 to 3 groups independently selected from Ci^ alkyl optionally substituted with 1 to 5 halogen atoms, ORa or OC(0)Ra, In one subset R6a is selected from C02Ra, C(0)NHORa, methyltetrazolyl, methyloxadiazolyl, NRbC(0)NRbRc, and NRbC(0)0Ra. In a further subset R6a is selected from C02Ra, methyltetrazolyl and methyloxadiazolyl, In another embodiment of formula 1(2), R6C is hydgrogen or halogen. In one subset R6C is hydgrogen. In another embodiment R7a is hydrogen or halogen. In one subset R7a is hydrogen. In another subset R7a js fluorine. In yet another subset R6b is hydrogen, fluorine or chlorine, and R7a is hydrogen or fluorine. In another embodiment of formula 1(2) R5 is selected from C1-4 alkyl optionally substituted with 1 to 5 halogen atoms or a cyano group, C3-6 cycloalkyi, isoxazolyl, pyrimidinyl and pyridinyl (and N-oxide thereof) optionally substituted with halogen. wherein m is 0 or 1, R6a is 2-methyl-2H-tetrazol-5-yl, 3-methyI-l,2,4-oxadia2ol-5-yl, C02Ra or C(0)NHORa wherein Ra is C1-4 alkyl, particularly methyl; R6b is hydrogen, fluorine or chlorine; R3b is C1-4 alkyl, particularly methyl; R5 is selected from C1-4 alkyl optionally substituted with 1 to 5 halogen atoms or a cyano group, C3-6 cycloalkyl, isoxazoIyl, pyimidinyl and pyridinyl (and N-oxide thereof) optionally substituted with halogen or trifluoromethyl, particularly trifluoromethyl, difluoromethyl, chlorodifluromethyl, 2,2,2-trifluoroethyl, pentafluoromethyl, cyanomethyl, 5-pyrimidinyl, 5-isoxazolyl and 5-bromo-3-pyridinyl and N-oxide thereof; and R7a is hydrogen or fluorine. Some representative compounds are: stereoconfiguration at the indicated carbon, § R3a is CH3 Unless otherwise stated, the following terms have the meanings indicated below: "Alkyl" as well as other groups having the prefix "alk" such as, for example, alkoxy, alkanoyl, alkenyl, alkynyl and the like, means carbon chains which may be linear or branched or combinations thereof. Examples of alkyl groups include methyl, ethyl, propyl, isopropyl, butyl, sec- and tert-butyl, pentyl, hexyl, heptyl and the like. "Alkenyl" means a linear- or branched carbon chain containing at least one C=C bond. Examples of alkenyl include allyl, 2-butenyl, 3-butenyl, l-methyl-2-propenyl, and the like, "Alkynyl" means a linear or branched carbon chain containing at least one C=C bond. Examples of alkynyl include propargyl, 2-butyny!, 3-butynyl, 1-methyI-2-propynyl, and the like. "Cyclic imide" includes succinimide, maleimide, phthalimide and the like. "Cycloalkyl" means carbocycles containing no heteroatoms, and includes mono-, bi- and tricyclic saturated carbocycles, as well as fused ring systems. Such fused ring systems can include one ring that is partially or fully unsaturated such as a benzene ring to form fused ring systems such as benzofused carbocycles. Cycloalkyl includes such fused ring systems as spirofused ring systems. Examples of cycloalkyi include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, decahydro-naphthalene, adamantanc, indanyl, indenyl, fluorenyl, 1,2,3,4-tetrahydronaphthalene and the like, "Haloalkyl" means an alkyl radical as defined above wherein at least one and up to all of the hydrogen atoms are replaced with a halogen. Examples of such haloalkyl radicals include chloromethyl, 1-bromoethyl, fluoromethyl, difluoromethyl, trifluoromethyl, 2;2,2-trifluoroethyl and the like. "Halogen" means fluorine, chlorine, bromine and iodine. "Optionally substituted" is intended to include both substituted and unsubstituted- Thus, for example, optionally substituted aryl could represent a pentafluorophenyl or a phenyl ring. Optica! Isomers - Diastereomers - Geometric Isomers - Tautomers Compounds described herein may contain an asymmetric center and may thus exist as enantiomers. Where the compounds according to the invention possess two or more asymmetric centers, they may additionally exist as diastereomers. The present invention includes all such possible stereoisomers as substantially pure resolved enantiomers, racemic mixtures thereof, as well as mixtures of diastereomers. The above Formula I is shown without a definitive stereochemistry at certain positions. The present invention includes all stereoisomers of Formula I and pharmaceutically acceptable salts thereof. Diastereoisomeric pairs of enantiomers may be separated by, for example, fractional crystallization from a suitable solvent, and the pair of enantiomers thus obtained may be separated into individual stereoisomers by conventional means, for example by the use of an optically active acid or base as a resolving agent or on a chiral HPLC column. Further, any enantiomer or diastereomer of a compound of the general Formula I may be obtained by stereospecific synthesis using optically pure starting materials or reagents of known configuration. Some of the compounds described herein contain olefmic double bonds, and unless specified otherwise, are meant to include both E and Z geometric isomers. Some of the compounds described herein may exist with different points of attachment of hydrogen, referred to as tautomers. Such an example may be a ketone and its enol form known as keto-enol tautomers. The individual tautomers as well as mixture thereof are encompassed with compounds of Formula I. Salts The term "pharmaceutically acceptable salts" refers to salts prepared from pharmaceutically acceptable non-toxic bases or acids. When the compound of the present invendon is acidic, its corresponding salt can be conveniently prepared from pharmaceutically acceptable non-toxic bases, including inorganic bases and organic bases. Salts derived from such inorganic bases include aluminum, ammonium, calcium, copper (ic and ous), ferric, ferrous, lithium, magnesium, manganese (ic and ous), potassium, sodium, zinc and the like salts. Preferred are the ammonium, calcium, magnesium, potassium and sodium salts. Salts prepared from pharmaceutically acceptable organic non-toxic bases-include salts of primary, secondary, and tertiary amines derived from both naturally occurring and synthetic sources. Pharmaceutically acceptable organic non-toxic bases from which salts can be ■J formed ir.clude, for example, arginine, betaine, caffeine, choline, N,N-dibenzyl- ethylenediamine, diethylamine, 2-diethylaminoethanoI, 2-dimethylaminoethanol, ethanolamine, ethylenediamine, N-ethylmorpholine, N-ethylpiperidine, glucamine, glucosamine, histidine, hydrabamine, isopropylamine, dicyclohexylamine, lysine, methylglucamine, morpholine, piperazine, piperidine, polyamine resins, procaine, purines, theobromine, triethylamme, trimethylamine, tripropylamine, tromethamine and the like. When the compou-nd of the present invention is basic, its corresponding salt can be conveniently prepared from pharmaceutically acceptable non-toxic inorganic and organic acids. Such acids include, for example, acetic, benzenesulfonic, benzoic, camphorsulfonic, citric, ethanesulfonic, fumaric, gluconic, glutamic, hydrobromic, hydrochloric, isethionic, lactic, maleic, malic, mandelic, methanesulfonic, mucic, nitric, pamoic, pantothenic, phosphoric, succinic, sulfuric, tartaric, p-toluenesulfonic acid and the like. Preferred are citric, hydrobromic, hydrochloric, maleic, phosphoric, sulfuric, and tartaric acids. Prodrugs The present invention includes within its scope prodrugs of the compounds of this invendon. In general, such prodrugs will be functional derivatives of the compounds of this invention which are readily convertible in vivo into the required compound. Thus, in the methods of treatment of the present invention, the term "administering" shall encompass the treatment of the various conditions described with the compound specifically disclosed or with a compound which may not be specifically disclosed, but which converts to the specified compound in vivo after admimstration to the patient Conventional procedures for the selection and preparation of suitable prodrug derivatives are described, for example, in "Design of Prodrugs," ed, H. Bundgaard, Elsevier, 1985. Metabolites of these compounds include active species produced upon introduction of compounds of this invention into the biological milieu. Pharmaceutical Compositions Another aspect of the present invention provides pharmaceutical compositions which comprises a compound of Formula I and a pharmaceutically acceptable carrier. The term "composition", as in pharmaceutical composition, is intended to encompass a produc: comprising the active ingredient(s), and the inert ingredient(s) (pharmaceutically acceptable excipients) that make up the carrier, as well as any product which resul:s, directly or indirectly, from combination, complexation or aggregation of any two or more of the ingredients, or from dissociation of one or more of the ingredients, or from other types of reactions or interactions of one or more of the ingredients. Accordingly, the pharmaceutical compositions of the present invention encompass any composition made by admixing a compound of Formula I, additional active ingredient(s), and pharmaceutically acceptable excipients. The pharmaceutical compositions of the present invention comprise a compound represented by Formula I (or pharmaceutically acceptable salts thereof) as an active ingredient, a pharmaceutically acceptable carrier and optionally other therapeutic ingredients or adjuvants. The compositions include compositions suitable for oral, rectal, topical, and parenteral (including subcutaneous, intramuscular, and intravenous) administration, although the most suitable route in any given case will depend on the particular host, and nature and severity of the conditions for which the active ingredient is being administered. The pharmaceutical compositions may be conveniently presented in unit dosage form and prepared by any of the methods well known in the art of pharmacy. In practice, the compounds represented by Formula I, or pharmaceutically acceptable salts thereof, of this invention can be combined as the active ingredient in intimate admixture with a pharmaceutical carrier according to conventional pharmaceutical compounding techniques. The carrier may take a wide variety of forms depending on the form of preparation desired for administration, e.g., oral or parenteral (including intravenous). Thus, the pharmaceutical compositions of the present invention can be presented as discrete units suitable for oral administration such as capsules, cachets or tablets each containing a predetermined amount of the active ingredient. Further, the compositions can be presented as a powder, as granules, as a solution, as a suspension in an aqueous liquid, as a non-aqueous liquid, as an oil-in-water emulsion or as a water-in-oil liquid emulsion. In addition to the common dosage forms set out above, the compound represented by Formula I, or pharmaceutically acceptable salts thereof, may also be administered by controlled release means and/or delivery devices. The compositions may be prepared by any of the methods of pharmacy. In general, such methods include a step of bringing into association the active ingredient with the carrrier that constitutes one or more necessary ingredients. In general, the compositions are prepared by unifonnly and intimately admiring the active ingredient with liquid carriers or finely divided solid earners or both. The product car. then be conveniently shaped into the desired presentation. Thus, the pharmaceutical compositions of this invention may include a pharmaceutically acceptable earner and a compound or a pharmaceutically acceptable salt of Formula I. The compounds of Formula I, or pharmaceutically acceptable salts thereof, can also be included in pharmaceutical compositions in combination with one or more other therapeutically acdve compounds. The pharmaceutical carrier employed can be, for example, a solid, liquid, or gas. Examples of solid carriers include lactose, terra alba, sucrose, talc, gelatin, agar, pectin, acacia, magnesium stearate, and stearic acid. Examples of liquid carriers are sugar syrup, peanut oil, olive oil, and water. Examples of gaseous carriers include carbon dioxide and nitrogen. In preparing the compositions for oral dosage form, any convenient pharmaceutical media may be employed. For example, water, glycols, oils, alcohols, flavoring agents, preservatives, coloring agents and the like may be used to form oral liquid preparations such as suspensions, elixirs and solutions; while carriers such as starches, sugars, microcrystalline cellulose, diluents, granulating agents, lubricants, binders, disintegrating agents, and the like may be used to form oral solid preparations such as powders, capsules and tablets. Because of their ease of administration, tablets and capsules are the preferred oral dosage units whereby solid pharmaceutical carriers are employed. Optionally, tablets may be coated by standard aqueous or nonaqueous techniques A tablet containing the composition of this invention may be prepared by compression or molding, optionally with one or more accessory ingredients or adjuvants. Compressed tablets may be prepared by compressing, in a suitable machine, the active ingredient in a free-flowing form such as powder or granules, optionally mixed with a binder, lubricant, inert diluent, surface active or dispersing agent. Molded tablets may be made by molding in a suitable machine, a mixture of the powdered compound moistened with an inert liquid diluent. Each tablet preferably contains from about O.lmg to about 500mg of the active ingredient and each cachet or capsule preferably containing from about O.1mg to about 500mg of the active ingredient. Pharmaceutical compositions of the present invention suitable for parenteral administration may be prepared as solutions or suspensions of the active compounds in water, A suitable surfactant can be included such as, for example, hydroxypropylcellulose. Dispersions can also be prepared in glycerol, liquid polyethylene glycols, and mixtures thereof in oils. Further, a preservative can be included to prevent the detrimental growth of microorganisms. Pharmaceutical compositions of the present invention suitable for injectable use include sterile aqueous solutions or dispersions. Furthermore, the compositions can be in the form of sterile powders for the extemporaneous preparation of such sterile injectable solutions or dispersions. In all cases, the final injectable form must be sterile and must be effectively fluid for easy syringability. The pharmaceutical compositions must be stable under the conditions of manufacture and storage; thus, preferably should be preserved against the contaminating action of microorganisms such as bacteria and fungi. The carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (e.g. glycerol, propylene glycol and liquid polyethylene glycol), vegetable oils, and suitable mixtures thereof. Pharmaceutical compositions of the present invention can be in a form suitable for topical use such as, for example, an aerosol, cream, ointment, lotion, dusting powder, or the like. Further, the compositions can be in a form suitable for use in transdermal devices. These formulations may be prepared, utilizing a compound represented by Formula I of this invention, or pharmaceutically acceptable salts thereof, via conventional processing methods. As an example, a cream or ointment is prepared by mixing hydrophilic material and water, together with* about 5 wt% to about 10 wt% of the compound, to produce a cream or ointment having a desired consistency. Pharmaceutical compositions of this invention can be in a form suitable for rectal administration wherein the carrier is a solid. It is preferable that the mixture forms unit dose suppositories. Suitable carriers include cocoa butter and other materials commonly used in the art. The suppositories may be conveniently formed by first admixing the composition with the softened or melted carrier(s) followed by chilling and shaping in moulds. . In addition to the aforementioned carrier ingredients, the pharmaceutical formulations described above may include, as appropriate, one or more additional carrier ingredients such as diluents, buffers, flavoring agents, binders, surface-active agents, thickeners, lubricants, preservatives (including anti-oxidants) and the like. Furthermore, other adjuvants can be included to render the formulation isotonic with the blood of the intended recipient. Compositions containing a compound described by Formula I, or pharmaceutically acceptable salts thereof, may also be prepared in powder or liquid concentrate form. The following are examples of representative pharmaceutical dosage forms for the compounds of Formula I: Injectable Suspension (I.M.) mg'mL Compound of Formula 1 10 Methylcellulose 5.0 Tween 80 0.5 Benzyl alcohol 9.0 Benzalkonium chloride 1.0 Water for injection to a total volume of 1 mL Tablet mg tablet Compound of Formula I 25 Microcrystalline Cellulose 415 Povidone 14.0 Pregeladnized Starch 43,5 Magnesium Stearate 2.5 500 Capsule mg/capsule Compound of Fonnula I 25 Lactose Powder 573^ Magnesium Stearate 1,5 600 Utilities Compounds of this invention are antagonists or inverse agonists of bradykinin receptor, in particular the bradykinin B1 receptor, and as such are useful in the treatment and prevention of diseases and conditions mediated through the bradykinin receptor pathway such as pain and inflammation. The compounds would be effective in the treatment or prevention of pain including, for example, visceral pain (such as pancreatitis, interstitial cystitis, renal colic), neuropathic pain (such as postherpetic neuralgia, nerve injury, the "dynias", e.g., vulvodynia, phantom limb pain, root avulsions, painful traumatic mononeuropathy, painful polyneuropathy), central pain syndromes (potentially caused by virtually any lesion at any level of the nervous system), and postsurgical pain syndromes (eg, postmastectomy syndrome, postthoracotomy syndrome, stump pain)), bone and joint pain (osteoarthritis), repetitive motion pain, dental pain, cancer pain, myofascial pain (muscular injury, fibromyalgia), perioperative pain (general surgery, gynecological), chronic pain, dysmennorhea, as well as pain associated with angina, and inflammatory pain of varied origins (e.g. osteoarthritis, rheumatoid arthritis, rheumatic disease, tenosynovitis and gout). Further, the compounds of this invention can also be used to treat hyperreactive airways and to treat inflammatory events associated with airways . disease e.g. asthma including allergic asthma (atopic or non-atopic) as well as exercise-induced bronchoconstriction, occupational asthma, viral- or bacterial exacerbation of asthma, other non-allergic asthmas and "wheezy-infant syndrome'.' Compounds of the present invention may also be used to treat chronic obstructive pulmonary disease including emphysema, adult respiratory distress syndrome, bronchitis, pneumonia, allergic rhinitis (seasonal and perennial), and vasomotor rhinitis. They may also be effective against pneumoconiosis, including aluminosis, anthracosis, asbestosis, chalicosis, ptilosis, siderosis, silicosis, tabacosis and byssinosis. Compounds of the present invention may also be used for the Ireatment of inflammatory bowel disease including Crohn's disease and ulcerative colitis, irritable bowel syndrome, pancreatitis, nephritis, cystitis (interstitial cystitis), uveitis, inflammatory skin disorders such as psoriasis and eczema, rheumatoid arthritis and edema resulting from trauma associated with bums, sprains or fracture, cerebral edema and angioedema. They may be used to treat diabetic vasculopathy, diabetic neuropathy, diabetic retinopathy, post capillary resistance or diabetic symptoms associated with insulitis (e.g. hyperglycenmia, diuresis, proteinuria and increased nitrite and kallikrein urinary excretion). They may be used as smooth muscle relaxants for the treatment of spasm of the gastrointestinal tract or uterus. Additionally, they may be effective against liver disease, multiple sclerosis, cardiovascular disease, e.g. atherosclerosis, congestive heart failure, myocardial infarct; neurodegenerative diseases, eg. Parkinson's and Alzheimers disease, epilepsy, septic shock e.g. as anti-hypovolemic and/or anti-hypotensive agents, headache including cluster headache, migraine including prophylactic and acute use, closed head trauma, cancer, sepsis, gingivitis, osteoporosis, benign prostatic hyperplasia and hyperactive bladder. Animal models of these diseases and conditions are generally well known in the art, and may be suitable for evaluating compounds of the present invention for their potential utilities. Finally, compounds of the present invention are also useful as research tools (in vivo and in vitro). The compounds of this invention are useful in the treatment of pain and inflammation by the administration of a tablet, cachet, or capsule each containing, for example, O.lmg, O.5mg, Img, 3mg, 5mg, lOmg, 25mg, 50mg, lOOmg, 125mg, 250mg, or 500mg of a compound of this invention once every three to four hours, once, twice or three times a day, or (in an extended release formulation) once, twice or three times a week. The compounds would be effective in the treatment or prevention of pain including, for example, bone and joint pain (osteoarthritis), repetitive motion pain, dental pain, cancer pain, myofascial pain (muscular injury, fibromyalgia), perioperative pain (general surgery, gynecological) and chronic pain by the administration of a tablet, cachet, or capsule each containing, for example, O.lmg, 0.5mg, Img, 3mg, 5mg. lOmg, 25mg. 50mg, lOOmg, 125mg, 250mg, or 500mg of a compound of this invention once every three to four hours, once, twice or three times a day, or (in an extended release formulation) once, twice or three times a week. In particular, inflammatory pain such as, for example, inflammatory airways disease (chronic obstructive pulmonary disease) would be effectively treated « by the compounds of this invention by the administration of a tablet, cachet, or capsule each containing, for example, O.lmg, 0.5mg, Img, 3mg, 5mg. lOmg, 25mg, 50rag, lOOmg, 125mg, 250mg, or 500mg of a compound of this invention once every three to four hours, once, twice or three times a day, or (in an extended release formulation) once, twice or three times a week-Further, the compounds of this invention can additionally be used to treat asthma, inflammatory bowel disease, rhinitis, pancreatitis, cystitis (interstitial cystitis), uveitis, inflammatory skin disorders, rheumatoid arthritis and edema resulting from trauma associated with bums, sprains or fracture by the administration of a tablet, cachet, or capsule each containing, for example, O.lmg, 0.5mg, Img, 3mg, 5mg, lOmg, 25mg, 50mg, lOOmg, 125mg, 25Qmg, or 500mg of a compound of this invention once every three to four hours, once, twice or three times a day, or (in an extended release formulation) once, twice or three times a v/eek. They may be used subsequent to surgical interv'ention (e.g. as postoperative analgesics) and to treat inflammatory pain of varied origins (e.g. osteoarthrids, rheumatoid arthritis, rheumatic disease, teno-synovitis and gout) as well as for the treatment of pain associated with angina, menstruation or cancer by the administration of a tablet, cachet, or capsule each containing, for example, O.lmg, 0.5mg, Img, 3mg, 5mg, lOmg, 25mg, 50mg, lOOmg, 125mg, 250mg, or 500mg of a compound of this invention once every three to four hours, once, twice or three times a day, or (in an extended release formulation) once, twice or three times a week. They may be used to treat diabetic vascuiopathy, post capillary resistance or diabetic symptoms associated with insulitis (e.g. hyperglycemia, diuresis, proteinuria and increased nitrite and kallikrein urinary excretion) by the administration of a tablet, cachet, or capsule each containing, for example, O.lmg, 0.5mg, Img, 3mg, 5mg, lOmg, 25mg, 50mg, lOOmg, 125mg, 250mg, or 500mg of a compound of this invention once every three to four hours, once, twice or three times a day, or (in an extended release formulation) once, twice or three times a week. They may be used to treat inflammatory skin disorders such as psoriasis and eczema by the administration of a tablet, cachet, or capsule each containing, for example, O.lmg, 0.5mg, Img, 3mg, 5mg, lOmg, 25mg, 50mg, lOOmg, 125mg, 250mg, or 500mg of a compound of this invention once every three to four hours, once, twice or three times a day, or (in an extended release formuladon) once, twice or three times a week- They may be used as smooth muscle relaxants for the treatmeric of spasm of the gastrointestinal tract or uterus or in the therapy of Crohn's disease,. ulcerative colitis or pancreatitis by the administration of a tablet, cachet, or capsule each containing, for example, O.lmg, 0.5mg, Img, 3mg, 5mg, lOmg, 25mg, 50mg, lOOmg, 125mg, 250mg, or 500mg of a compound of this invention once every three to four hours, once, twice or three times a day, or (in an extended release formulation) once, twice or three times a week. Such compounds may be used therapeutically to treat hyperreactive airways and to treat inflammatory events associated with airways disease e.g. asthma, and to control, restrict or reverse air-vays hyperreactivity in asthma by the administration of a tablet, cachet, or capsule each containing, for example, O.lmg, 0.5mg, Img, 3mg, 5mg, lOmg, 25mg, 50mg, lOOmg, 125mg, 250mg, or 500mg of a compound of this invention onc^ every three to four hours, once, twice or three times a day, or (in an extended release formulation) once, twice or three times a week. They may be usee to treat intrinsic and extrinsic asthma including allergic asthma (atopic or non-atopic) as well as exercise-induced broncho-constriction, occupational asthma, viral or bacterial exacerbated asthma, other non-allergic asthmas and "wheezy-infant syndrome" by the administration of a tablet, cachet, or capsule each containing, for example, O.lm.g, 0-5mg, Img, 3mg, 5mg, lOmg, 25mg, 50mg, iOOmg, 12f mg, 250mg, or 500mg of a compound of this invention once every three to four hours, once, twice or three times a day, or (in an extended release formulation) or.:e, twice or three times a week. They may also be effective against pneumoconiosis, including aluminosis, anthracosis, asbestosis, chalicosis, pdlosis, siderosis, silicosis, tabacosis and byssinosis was well as adult respiratory distress syndrome, chronic obstructive pulmonary or airways disease, bronchitis, allergic rhinitis, and vasomotor rhinitis by the administration of a tablet, cachet, or capsule each containing, for example, 0. Img, 0.5mg, Img, 3mg, 5mg, lOmg, 25mg, 50mg, lOOmg, 125mg, 250mg, or 500mg of a compound of this invention once every three to four hours, once, twice or three times a day, or (in an extended release formulation) once, twice or three times a week. Additionally, they may be effective against liver disease, multiple sclerosis, atherosclerosis, Alzheimer's disease, septic shock e.g. as anti-hypovolemic and/or and-hypotensive agents, cerebral edema, headache including cluster headache, migraine including prophylactic and acute use, closed head trauma, irritable bowel syndrome and nephritis by the administration of a tablet, cachet, or capsule each containing, for example, O.lmg, 0.5mg, Img, 3mg, 5mg, lOmg, 25mg, 50mg,,100mg, 125mg, 250mg, or 500mg of a compound of this invention once every three to four hours, once, twice or three times a day, or (in an extended release formulation) once, twice or three times a week. Combination Therapy Compounds of Formula I may be used in combination with other drugs that are used in the treatment/prevention/suppression or amelioration of the diseases or conditions for which compounds of Formula I are useful. Such other drugs may be administered, by a route and in an amount commonly used therefor, contemporaneously or sequentially with a compound of Formula I. When a compound of Formula I is used contemporaneously with one or more other drugs, a pharmaceutical composition conraining such other drugs in addition to the compound of Formula I is preferred. Accordingly, the pharmaceutical compositions of the present invention include those tiiat also contain one or more other active ingredients, in addition to a compound of Formula I. Examples of other acdve ingredients that may be combined with a compound of Formula I, either administered separately or in the same pharmaceutical composidons, include, but are not limited to: (1) morphine and other opiate receptor agonists including propoxyphene (Darvon); (2) non-steroidal antiinflammatory drugs (NSAIDs) including COX-2 inhibitors such as propionic acid derivatives (alminoprofen, benoxaprofen, bucloxic acid, carprofen, fenbufen, fenoprofen, fluprofen, flurbiprofen, ibuprofen, indoprofen, ketoprofen, miroprofen, naproxen, oxaprozin. pirprofen, pranoprofen, suprofen, tiaprofenic acid, and tioxaprofen), acedc acid derivatives (indomethacin, acemetacin, alclofenac, clidanac, diclofenac, fenclofenac. fenclozic acid, fentiazac, furofenac, ibufenac, isoxepac, oxpinac, sulindac, dopinac, tolmetin, zidometacin, and zomepirac), fenamic acid derivadves (flufenamic acid, meclofenamic acid, mefenamic acid, niflumic acid and tolfenamic acid), biphenylcarboxylic acid derivatives (diflunisal and flufenisal), oxicams (isoxicam, piroxicam, sudoxicam and tenoxican), salicylates (acetyl salicylic acid, sulfasalazine) and the pyazoiones (apazone, bezpiperylon, feprazone, mofebutazone, oxyphenbutazone. phenylbutazone), and the coxibs (celecoxib, valecoxib, rofecoxib and etoricoxib); (3) corticosteroids such as betamethasone, budesonide, cortisone, dexamethasone, hydrocortisone, methylprednisolone, prednisolone, prednisone and triamcinolone; (4) histamine HI receptor antagonists such as bromopheniran\ine, chlorpheniramine, dexchlorpheniramine, triprolidine, clemastine, diphenhydramine, diphenylpyraline, tripelennamine, hydroxyzine,' methdilazine, promethazine, trimeprazine, azatadine, cyproheptadine, antazoline, pheniramine pyrilamine, astemizole, terfenadine, loratadine, cetirizine, desloratadine, fexofenadine and levocetirizine; (5) histamine H2 receptor antagonists such as cimetidine, famotidine and ranitidine; (6) proton pump inhibitors such as omeprazole, pantoprazole and esomeprazole; (T)leukotriene antagonists and 5-lipoxygenase inhibitors such as zafiriukast, mor.:elukast, pranlukast and zileuton; (8) drugs used for angina, myocardial ischemia including nitrates such as nitroglycerin and isosorbide nitrates, beta blockers such as ater.^lol, metoprolol, propranolol, acebutolol ,betaxolol, bisoprolol, carteolol, labetalol, nadolol, oxprenolol, penbutolol, pindolol, sotalol and dmolol, and calcium channel blockers such as dildazam, verapamil, nifedipine, bepndil, teiodipine, tlunanzine. isradipine, nicardipine and nimodipine; ly) incontinence medications such as antimuscarinics, e.g., tolterodine and oxybutinin); (10) gastrointestinal antispasmedies (such as atropine, scopolamine, dicyclomine, antimuscarinics, as well as diphenoxylate); skeletal muscle relaxants (cyclobenzaprine, carisoprodol. chlorphenesin, chlorzoxazone, metaxalone, methocarbamol, baclofen, dantrolene, diazepam, or orphenadrine); (11) gout medications such as allopurino!. probenicid and colchicine; (12) drugs for rheumatoid arthritis such as methotrexate, auranofin, aurothioglucose and gold sodium thiomalate; (13) drugs for osteoporosis such as alendronate and raloxifene; decongestants such as pseudoep-.edrine and phenylpropanolamine; (14) local anesthetics; (15) and-herpes drugs such as acyclovir, valacyclovir and famcyclovir; and (15) anti-emetics such as ondansetron and granisetron. Biological Evaluation Assessing the Affinity of Selected Compounds to Bind to the Bradykinin B1 or B2 Receptor Radioligand binding assays are performed using membranes from CHO cells that stably express the human, rabbit, rat, or dog B1 receptors or CHO cells that express the human B2 recep'or. For all receptor types, cells are harvested from culture flasks in PBS/lmM EDTA and centrifuged at 1000x2 for 10 minutes. The cell pellets are homogenized with a polytron in ice cold 20mM HEPES, InaM EDTA, pH 7.4 (lysis buffer) and centrifuged at 20,000xg for 20 minutes. The membrane pellets are rehomogenized in lysis buffer, centrifuged again at 20,000xg and the final pellets are resuspended at 5mg protein/ml in assay buffer (120mM NaCl, 5mM KCl, 20mM HEPES, pH 7.4) supplemented with 1% BSA and frozen at-80°C. On the day of assay, membranes are centrifuged at 14,000xg for 5 minutes and resuspended to the desired protein concentration in assay buffer containing lOOnM enaliprilat, 140µg/mL bacitracin and 0,1% BSA. 3H-des-arglO, leu9 kallidin is the radioligand used for the human and rabbit B1 receptors, 3H-des-arglO kallidin is used for the rat and dog Bl receptors, and 3H-bradykinin is used to label the human B2 receptor. For all assays, compounds are diluted from DMSO stock solutions with 4µL added to assay tubes for a final DMSO concentration of 2%. This is followed by the addition of lOOµL radioligand and lOOµL of the membrane suspension. Nonspecific binding for the Bl receptor binding assays is determined using lµM des-arglO kallidjn and nonspecific binding for the B2 receptor is determined with lµM bradykin. Tubes are incubated at room temperature (220C) for 60 minutes followed by filtranon using a Tomtec 96-well harvesting systeni. Radioactivity retained by the filler is counted using a Wallac Beta-plate scintillation counter. The compounds of this invention have affinity for the Bl receptor in the above assay as demonstrated by results of less than SpcNL It is advantageous that the assay results be less than lµM, even more advantageous for the results be less than 0.5MM. It is further advanrageous that compounds of this invention have affinity for the bradyidnin Bl receptor over the bradykinin B2 receptor; more advantageously, the affinity for the Bl receptor is at least 10 fold, and preferably over 100 fold, over that for the B2 receptor. Assay for Bradykinin Bl Antagonists Bl agonist-induced calcium mobilization was monitored using a Fluorescence Imaging Plate Reader (FLIPR). CHO cells expressing the Bl receptor were plated in 96 or 384 well plates and allowed to incubate in Iscove's modified DMEM overnight. Wells were washed two times with a physiological buffered salt solution and'then incubated with 4uM Fluo-3 for one hour at 370C. The plates were then washed two times with buffered salt solution and lOOuL of buffer was added to each well. Plates were placed in the FLIPR unit and allowed to equilibrate for two minutes. The test compound was then added in 50ul volumes followed five minutes later by 50ul of agonist (des-arglO kallidin). Relative fluorescence peak heights in the absence and presence of antagonist were used to calculate the degree of inhibition of the Bl receptor agonist response by the test compound. Eight to ten concentrations of test compound were typically evaluated to construct an inhibition curve and detennine IC50 values using a four-parameter nonlinear regression curve fitting routine. Assay for Bradykinin Inverse Agonists Inverse agonist acrivity at the hunaan Bl receptor was evaluated using transiently transfected HEK293 cells. One day following transfection cell flasks were labeled overnight with 6uCi/ml pHjmyo-inositoI. On the day of assay, the media was removed and the attached cells were gently rinsed with 2x20ml of phosphate-buffered saline. Assay buffer (HEPES buffered physiological salts, pH 7.4) was added and the cells were detached by tapping of the flask. The cells were centrifuged at 800xg for five minutes and resuspended a: lxl06 cells/ml in assay buffer supplemented with lOmM lithium chloride. After 10 minutes at room temperature, one-half ml aliquots were distributed to tubes containing test compound or vehicle. After an additional 10 minutes the tubes were transferred to a 37°C water bath for 30 minutes. The incubation was terminated by the addition of a 12% perchloric acid solution and the tubes were placed on ice for 30 .Tiinutes. The acid was then neutralized with KOH and the tubes centrifuged to pellet precipitated material [3H]Inosito[ monophosphate formed was recovered by standard ion exchange chromatographic techniques and quantitated by liquid scintillation counting. Inverse agonist activity was determined by the degree to which a test compound reduced basal (cells incubated with vehicle) levels of [3H]inositol monophosphate accumulation. Abbreviations Used The following abbreviations have the meanings indicated, unless stated otherwise in the specification: BOC (boc) t-butyloxycarbonyl DCM dichloromethane DMF dimethylformamide DMSO Dimethyl sulfoxide EDC or EDCI l-(3-dim.ethyIaminopropyl)3-ethylcarbodiimide HCl eq. equivalen.t(s) ES (or ESI) - MS electron spray ionization - mass spectroscopy Et ethyl EtOAc ethyl acetate EtOH ethane! FAB-MS fast atom bombardment-mass spectroscopy HOBt 1-hydroxybenzotriazole hydrate HPLC high pressure liquid chromatography LCMS Liquid chromatography/mass spectroscopy LHMDS lithium bis(triraethylsilyl)amide Me methyl MeOH Methanol MHz megahertz MsCl Mesyl chloride NEt3 Triethylamine NMR nuclear magnetic resonance TFA trifluoroacetic acid THF tetrahydrofuran Compounds of formula I may be prepared according to the following illustrative schemes. In Scheme 1, compound (la) is assembled by coupling the biaryhnethanamine derivative (1) to the protected aminocycloalkanoic acid (2) using standard peptide coupling reagent combinations, such as EDCI/HOBt, in an appropriate solvent, such as THF, to provide (3). The Boc protecting group is then removed by the action of an acid, like HCl, in an appropriate solvent, like MeOH, to yield an ammonium salt from which the free-base derivative (4) may be obtained using an appropriate base, such as ammonia, and an appropriate solvent, such as chloroform. This amine deriva:ive (4) is then reacted with a carboxylic acid or carboxylic acid equivalent to yield title compound (la). Alternatively, the acid-salt of (4) can be used in the final reaction to yield title compound (la) provided an appropriate base such as triethylamine is added. Alternatively, compound (la) may be assembled by coupling the biarylmethanamine derivati\e (2), with the acylated aminocycloalkanoic acid (5) as shown in Scheme la. In Scheme 2a, the cyanobiaryl derivative (S) is assembled using a Suzuki reaction between an aromatic boronic acid derivative (6), or an appropriate boronic ester derivative, and an aromatic halide (7) in the presence of a triarylphosphine, like triphenylphosphine, and a metal catalyst, like palladiuna acetate. The resultant cyano biaryl intermediate (8) is then catalytically reduced to the corresponding amine biaryl derivative (la) using hydrogen and a metal, such as Raney Ni, in an appropriate solvent. Alternatively, as illustrated in Scheme 2b, a methanamine derivative (9), after primary amine protection with an appropriate protecting group such as Boo, is elaborated to the pinacol boron ester (11) using a palladium catalyst in an appropriate solvent, like dimethyl sulfoxide. This boron ester (11) is coupled^to an aryl halide derivative (7) employing Suzuki reaction conditions to yield (1). A third method for the preparation of biarylmethanamine derivatives is depicted in Scheme 2c. The biaryl moiety (14) is first assembled using a palladium catalyzed coupling of (12) with an aryl zinc compound (13) as shown. The methyl group of biaryl (14) is then elaborated according to the three step sequence of halogenation, nucleophilic displacement of the halogen with azide, and reduction to provide the corresponding amine intermediate (la). Alternatively, the biarylmethanamine (la) can also be prepared starting from the arylcarbonitrile (16) and aryl zinc compuond (13) as previously discussed. The resulting biarylcarbonitrile (8) is then reduced using hydrogen to provide (la). It will be appreciated by persons skilled in the art that functional group interconversion can be used to provide various compounds of formula I. As illustrated in Scheme 3, derivative (3a) is bis-deprotected first by the action of a strong acid, like TFA, and second by alkaline hydrolysis in a suitable mixture of water and an organic solvent, like methanol, at a temperature between 25 and 100 °C to yield the amino acid derivative (17). Prior activation of a carboxylic acid (R5COOH) with an appropriate set of peptide coupling reagents, like EDCI/HOBt, forms the 'active ester' which then reacts with the amino acid derivative (17) to yield (18). The latter compound can either react with amines (HNRbRc) or alkyloxy amines (H2N0Ra) under the action of an appropriate set of peptide coupling reagents, like EDCI/HOBt, to form the claimed compounds (lb) and (Ic), respectively. N-alkylation is illustrated in Scheme 4. The amine (4) is alkylated with excess alkyl iodide (I-Rl) in an appropriate solvent, like THF, in the presence of The preparation of compounds of fonnula I having a 1,2-cis- or 1,2-trans-cyclopropyl moiety is illustrated in Schemes 5 and 6, According to known procedures (K. Burgess et al., J. Org. Chem., 57:5931-5936(1992)), di-ttert-butyl malonate is elaborated to derivative (20). The N-Boc group is removed using methane sulfonic acid according to L. S. Lin et al. Tetrahedron Lett, 41:7013-7016(2000) to give amine (21). This amine is allowed to react with a carboxylic acid or carboxylic acid equivalent under appropriate peptide coupling conditions to yield (22). The tert-, butyl ester is then cleaved with an acid, like TFA, in an appropriate solvent, like DCM, to provide acid (23). Biarylmethanamine (1) is then coupled with the acid (23) using an appropriate set of peptide coupling reagents, like EDCI/HOBt, to produce the title compound (le). Further elaboration of (le) to additional compounds of formula I may be accomplished using procedures well known to those skilled in the art. For example, the acetyl group may be removed by hydrolysis to provide the con'esponding alcohol; the alcohol may be convened to the corresponding sulfonate by treatment with sulfonyl chloride, and the sulfonate may be converted to the corresponding halide by treatment with a source of the halide. These and other functional transformations to provide compounds of formula I are described in typical organic chemistry textbooks such as March's Advanced Organic Chemistry: Reactions, Mechanisms, and Structure. 5th Ed., John Wiley & Sons, 2000. In Scheme 6, according to known procedures (K. Burgess et al.,7. Org. Chem., 57:5931-5936(1992)), di-tert-butyl malonate is elaborated to derivative (24). The N-Boc group is removed using an acid, like TFA, in an appropriate solvent, like DCM. This amine is allowed to react with a carboxylic acid or carboxylic acid equivalent under appropriate peptide coupling conditions, like EDCI/H0Bt/NEt3 to yield (25). Biarylmethanamine (1), is then allowed to open the lactone (25) in an appropriate aprotic solvent, like D!vIF, at a temperature between 20 and 100 °C, to produce the title compound (If). Further elaboration of (If) to additional title compounds may be accomplished using procedures well known to those skilled in the art as previously discussed. The following examples are provided to illustrate the invention without limiting the invention to the particulars of these examples. Compounds were named using: ACD/Name version 4.53 (Advanced Chemistry Development Inc. © 1994-2000). Address: 90 Adelaide Street West, Toronto, Ontario, M5H 3V9, Canada. EXAMPLE 1 Methyl 3-fluoro-4.{(lR)-l-[({ l-[(3,3,3-trifluoropropanoyl)ajnino]cyclopropyl}-carbonyI)amino]ethyl} -1,1 '-biphenyl-2-carboxylate Commercially available (lR)-l-(4-bromophenyl)ethanamine was Boc protected, using standard procedures known to those skilled in the art, to produce tert-butyl (lR)-l-(4-bromophenyl)e:hylcarbamate. To a solution of tert-butyl (lR)-l-(4-bromophenyl)ethylcarbamate (7.6 g, 25.3 mmol) in DMSO (20 mL) was added bis(pinacolato)diboron (7.07 g, 27.9 mmol), dichloro[l,1-bis(diphenylphosphino)ferrQcene]palIadium (II) dichloro- methane adduct (2.06 g, 2.53mmol), and potassium acetate (7.45 g, 76.0 mmol) at room temperature under N2. The resulting mixture was heated at 80 °C for 1 hour. The reaction was quenched by addition of EtOAc and filtered through celite. The organic extract was washed with water three times, saturated NaCl, dried over MgS04, filtered and concentrated under vacuum. The residue was purified on silica gel eluted with 0-20% ethyl acetate in hexane to provide tert-butyl (lR)-l-[4-(4,4,5,5-tetramethyI-l,3,2-dioxaborolan-2-yl)phenyl]ethyIcarbamate as a clear light yellow oil with a mass ion (ES+) of 333. To a stirred solution of tert-bntyl (lR)-l-[4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)phenyI]ethyIcarbamate (1.0 g, 2.9 mmol) and methyl 2-fluoro-6-iodobenzoate (1.2 g, 4.32 mmol) in 25 mL of a 5:1 THF:water mixture was added potassium carbonate (1.2 g, 8.64 mmol), tri-0-tolyIphosphine (350 mg, 1.15 mmol) and lastly palladium acetate (65 mg, 0.29 mmol). The reaction vessel was then sealed and placed into a 90 T oil bath for overnight stirring and heating. After about 18 hours the reaction mixture was cooled to ambient temperature and then diluted with EtOAc. The organics were washed with brine (x4), dried over sodium sulfate, filtered, and concentrated under reduced pressure to give an oil. This oil was subject to silica gel chromatography eluting with 10-60% EtOAc in hexanes to provide methyl 4'-{(lR)-I-[(tert-butoxycarbonyl)amino]ethyI}-3-fluoro-l,1-biphenyl-2-carboxylate (205 mg), found to be pure by LC/MS and proton NMR. Methyl 4-{(IR)-l-[(r(?rr-butoxycarbonyl)amino]ethy!}-3-fluoro-l,r-biphenyl-2-carboxylate (205 mg, 0.60 mmol) dissolved in MeOH (15 mL) was cooled to 0 °C. This homogenous solution was saturated with anhydrous hydrogen chloride and allowed to sit for 20 minutes. Dry nitrogen was then bubbled through the solution for about 30 minutes. Solvent was then removed under reduced pressure to yield an oilv residue. The oil was then dissolved in DCM and the solvent removed. This process was repeated until a solid amine hydrochloride was obtained. The above amine hydrochloride (85 mg, 0.27 mmol) along with 1- [(tert-butoxycarbonyl)amino]cyclopropanecarboxylic acid (55 mg, 0.27 mmol), H0BfH20 (8.4 mg, 0.05 mmol) and triethylamine (33 mg, 0.33 mmol) were dissolved in 4.5 mL of THF. To this room-temperature solution was added EDCI (74 mg, 0.38 mmol). After overnight stirring (ca. 16.5 h) the reacdon mixture was diluted with water and EtOAc. The organic layer was washed successively with IN HCl, 5% sodium bicarbonate, half-brine (x3) and then brine. The organic layer was dried over sodium sulfate, filtered and evaporated under reduced pressure to obtain a residue which was subjected to silica gel chromatography elating with 1-6% MeOH in DCM. Collection of product containing fractions and removal of solvent yielded 108 mg (86%) of methyl 4'-((lR)-l-[(( l-[(tert-butoxycarbonyl)amino]cyclopropyl)carbonyl)- amino]ethyl)-3-fluoro-l,r-biphenyl-2-carboxylate. Methyl 4'-{(lR}-l-[({ I-[(tert-butoxycarbonyI)amino]cyciopropyl}- carbonyl)amino]ethyl}-3-fluoro-l,r-biphenyl-2-carboxylate (108 mg, 0.24 mmol) dissolved in MeOH (5.0 mL) was cooled to 0 °C. This homogenous solution was saturated with anhydrous hydrogen chloride and allowed to sit for 30 minutes. Dry nitrogen was then bubbled through the solution for about 50 roin. Solvent was then removed under reduced pressure to yield an oily residue. The oil was then dissolved in DCM and the solvent removed. This process being repeated until a solid amine hydrochloride was obtained- The above amine hydrochloride (46 mg, 0.12 mmol) along with trifluoropropionic acid (15 mg, 0.12 mmol), H0Bt«H20 (3.6 mg, 0.02 mmol) and triethylamine (14 mg, 0.14 mmol) were dissolved in 1.6 mL of THF plus 1.6 mL of DIvIF. To this room-temperature solution was added EDCI (31 mg, 0.16 mmol). After overnight stirring (ca, 18 h) the reaction mixture was diluted with water and EtOAc. The organic layer was washed successively with IN HCl, 5% sodium bicarbonate, half-brine (x3) and then brine. The organic layer was dried over sodium sulfate, filtered and evaporated under reduced pressure to obtain a residue which was subjected to silica ge! chromatography eluting with 1-12% MeOH in DCM. Collection of product containing fractions and removal of solvent yielded 36 mg (67%) of the title compound as a foaming solid. Purity was determined by LCMS (ES MS, M+H+ found:467) and proton NMR (400 MHz, CD3OD : 5 7.555, 7.540, 7.535, 7.520, 7.515, 7.500, 7.393. 7.373, 7.319, 7.302, 7.298, 7.240, 7.222, 7.221, 7.211, 7. ISS, 7.167, 7.165, 5.116, 5.099. 5.081, 5.064, 3.659, 3.268, 3.241, 3.214, 3.1S7, 1.508, 1.490, 1.483, 1.477, 1.474, 1.470, 1.465, 1.454, 1.444, 1.056, 1.049, 1.036, 1.031, 1.023, 1.007,0.999,0.995,0.982,0.974). A solution of methyl 4-{(lR)-l-[({l-[(tert-butoxycarbonyl)amino]- cyclopropyl}carbonyl)amino]ethyl}-3-fluoro-1,1-biphenyl-2-carboxylate (466 mg, 1.0 mmol) in DCM (15 inL) and TFA (15 mL) was stirred under N2 for 20 minutes at ambient temperature, then the organic solvent was removed under vacuum. The residue was dissolved in MeOH (20 mL), 4N NaOH (10 mL) and water (10 mL). This mixture was heated at reflux for 4 hours and then neutralized with 6N HCl. Purification was achieved by preparative HPLC on a delta-pack Cig column, 300 A, pore size 15 µM with 0.05% HCI acid -aqueous acetonitrile solvent systems using various linear gradients. Fracdons containing product of 99% purity as measured by HPLC were combined and lyophilized to give 4-((lR)-l-{[(l-aminocyclopropyl)-carbonyl]amino}ethyl)-3-fluoro-1,1-biphenyl-2-carboxy]ic acid as a white solid. To a solution of trifluoropropionic acid (128 mg, 1.0 mmol) in DCM (1 mL), l-ethyl-(3-dimethylaniincpropyl)carbodiimide hydrochloride (229 mg, 1.2 mmol) and l-hydroxy-7-azabenzotriazole (136 mg, 1.0 mmol) were added. The resulting solution was stirred et room temperature for 20 minutes, then 4'-((lR)-l- {[(l-aminocyclopropyl)carbonyl]amino}ethyl)-3-fluoro-l,r-biphenyl-2-carboxylic acid (171 mg, 0.5 mmol) in mL DCM was added, followed by MA^-diisopropylethyl- amine until pH = 10 was achieved. The reaction mixture was stirred at ambient temperature under N2 for 2 hours, concentrated under vacuum and then partitioned between water and ethyl acetate. The organic extract was washed with brine, dried over anhydrous magnesium sulfate, filtered and concentrated under vacuum. The residue was subjected to co!urr.n chromatography on silica gel eluted with 40% MeOH in CHCI3. Collection and concentration of appropriate fractions provided 3- fluoro-4-{(lR)-l-[({l-[(3,33-:rifluoropropanoyl)amino]cyclopropyl}carbonyl)- amino]ethyl}-l,l-biphenyl-2-carboxylic acid as a white powder. To a solution of the above acid (226 mg, 0.50 mmol) in DCM (i mL), l-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride (134 mg, 0.70 mmol), 1- hydroxy-7-azabenzotriazole (6Smg, 0.50 mmol) and methoxyamine hydrochloride (167 mg, 1.0 mmol) were added, followed by N N-diisopropylethylamine until pH = 10 was achieved. The resulting solution was stirred at room temperature for 2 hours, and then partitioned between e:hyl acetate and water. The organic extract was washed with brine, dried over anhydrous magnesium sulfate, filtered and concentrated under vacuum. Purification was achieved by preparative HPLC on a delta-pack C18 column with 0.05% HCl acid -aqueous acetonitrile solvent systems using various linear gradients. Fractions containing product of 99% purity as measured by HPLC were combined and lyophilized to give the title compound as a white solid.. Purity was determined by LCMS (ES MS, M+H+ found:482) and proton NMR (400 MHz, DMS0-d6) 5 1.40 (d, J = 7.1 Hz, 3H), 0.60-0.80 (m, 2H), L27 (m, 2H), 3.23 (m, J = 11.2 Hz, 2H). 3.44 (s, 3H), 5.02 (q, 7= 8 Hz, 1H), 7.25-7.39 (m, 6H), 7.52 (m, 1H), 7.93 (d, j= 8.2Hz, 1H), 8.89 (s, 1H). EXAMPLE 3 Methyl 3-fluoro-4-{ l-methyl-l-[({ l-[(trifluoroacetyl)amino]cycIopropyl}-carbonyl)amino]ethyI} -1,1 -biphenyl-2-carboxylate Methyl 2-(4-bromopheny!)-2-methylpropanoate (3.75 g, 14.6 mmol, prepared according to J. Org. Chem., 59:2620-2622(1994)) in 150 mL of THF was allowed to react with potassium trimethylsilanolate (2.62 g, 20.4 mmol) at ambient temperature, for 60 hours, with continuous stirring. The reaction was then diluted with water and DCM. The pH of the aqueous phase was then adjusted to ca. 4, using IM HCl. The aqueous layer was then extracted three times with additional DCM. The organic layers were pooled, dried over sodium sulfate, filtered and then concentrated to obtain 3.59 g cf 2-(4-bromophenyl)-2-methylpropanoic acid, as a white solid, which gave LC/MS and proton NMR spectra consistent with theory. To 82 mL of toluene was added the above acid (2.00 g, 8.23 mmol), triethylamine (1.20 mL, 8.64 mmol) and diphenylphosphoryl azide (1.86 mL, 8.64 mmol). After refluxing this mixture under nitrogen for 1 hour, benzyl alcohol (1.70 mL, 16.5 mmol) was added and the reaction mixture was allowed to reflux overnight. Solvent was removed under reduced pressure and the resulting oil was diluted with ethyl acetate. The ethyl acetate solution was washed twice with 5% sodium bicarbonate and once with brine. The organic layer was dried over sodium sulfate, filtered and evaporated under reduced pressure to obtain a residue which wassubject to silica gel chromatography eluting with 0-2% MeOH in DCM. Collection of product containing fracdons and removal of solvent yielded 2.26 grams of benzyl N-[l-(4-bromophenyl)-l-methylethyl]carbamate, which gave LC/MS and proton NMR spectra consistent with theory. The above bromide (LOO g, 2.87 mmol) was added to 25 mL of DMF, followed by bis(pinacolato)boron (0.875 g, 3.45 mmol), potassium acetate (0.846 g, 8.62 mmol) and PdCl2(dppf)2CH2Cl2 (.063 g, 0.090 mmol). This mixture was heated to 80 °C, under nitrogen, for 3 hours. After cooling to ambient temperature, methyl 2-fluoro-6-iodobenzoate (0.965 g, 3.45 mmol), PdCl2(dppf)2'CH2Cl2 (.063 g, 0.090 mmol) and aqueous sodium carbonate (7.18 noL, 2M, 14.4 mmol) were added. This mixture was then heated to 80 °C overnight. After coolins to ambient temperature, most of the DMF was removed under reduced pressure and the biphasic mixture was diluted with ethyl acetate. The pH of the aqueous layer was made neutral with IM HCl, prior to extraction with two additional volumes of ethyl acetate. The combined organic layers were dried over sodium sulfate, filtered and evaporated under reduced pressure to obtain a residue which was subject to silica gel chromatography eluting with 0-2% MeOH in DCM. Collection of product containing fractions and removal of solvent yielded 0.25 grams of methyl 4'-(l-{[(benzyloxy)carbonyl]amino}-l-methylethyl)-3-fIuoro-l,r-biphenyI-2-carboxylate, which gave LC/MS and proton NMR spectra consistent with theory. Methyl 4'-(l-{ [(benzyloxy)carbonyl]amino}-l-methylethyl)-3-fluoro-l,r-biphenyl-2-carboxylate (0.25 g, 0.59 mmol) was dissolved in 6.0 mLof anhydrous ethanol. Pd/C (90 mg) was then added and the nitrogen atmosphere was exchanged for hydrogen. The reacdon mixture was allowed to stir for 72 hours. After filtration chough celite, the ethanol was removed under reduced pressure to yield 0.15 grams of methyl 4'-(l-amino-l-methylethyl)-3-fluoro-l,r-bipheny!-2-carboxylate which was of sufficient purity to use directly in the next reaction. The above-mentioned amine (80 mg, 0.28 mmol), was dissolved in anhydrous DCM (4 mL). To this stirred solution was added l-[(tert-butoxycarbonyl)-aminojcyclopropanecarboxylic acid (67 mg, 0.33 mmol), H0Bf-H20 (8 mg, 0.08 mmol) and lastly EDCI (69 g, 0.36 mmol). This mixture was allowed to stir overnight. Solvent was then removed under reduced pressure and the residue was subjected to silica gel chromatography eluting with a 1-3% MeOH in DCM gradient to provide methyl 4-{!-[({l-[(tert-butoxycarbonyl)amino]cyclopropyl}carbbnyl)-, amino]-l-methylethyl}-3-fluoro-l,r-biphenyl-2-carboxylate(82 mg), giving LC/MS and proton NMR spectra consistent with theory. The above material (82 mg, 0.17 mmol) dissolved in ethyl acetate (4 mL) was cooled to 0 °C. This homogenous solution was saturated with anhydrous hydrogen chloride and allowed to sit for 30 minutes- Dry nitrogen was then bubbled through the solution for about 30 minutes. Solvent was then removed under reduced pressure to yield an oily residue, which was used directly in the next reaction. To a 0 °C, stirred solution of the above mentioned amine (32 mg, 0.09 mmol) in DCM (2.0 mL) was added triethylamine (20 µL, 0.13 mmol) and lastly trifluoroacetic anhydride (20 µL, 0.11 mmol). After 10 minutes the ice bath was remove and stirring was continued for a total of 30 minutes The reaction mixture was subject to silica gel chromatography elating with 0.5-2% MeOH in DCM. Collection of product containing fractions and removal of solvent yielded 34 mg (84%) of the title compound as a foaming solid. Purity was determined by LCMS (ES MS, M + H+ found:467) and proton KMR (400 MHz, CD3OD : 5 7.552, 7.537. 7.531, 7.517, 7.512, 7.497. 7.442, 7.425, 7.420, 7.302, 7.297, 7.280, 7.250, 7.231, 7.203, 7.181, 7.159,3.644, 1.664, 1.444, 1.432, 1.424, 1.412, 1.080, 1.068, 1.060. 1.048). To a solution of 2-fluoro-6-iodobenzoic acid (iS.OOg, 56.39 mmol) in 150 mLCH2Cl2 containing 0.1 mLDMF was added oxalyl chloride (9.30g, 73.3 mmol) dropwise. The solution was stirred at room temperature for 75 minutes, then concentrated in vacuo. The residue was re-dissolved in 150 mL CH2CI2, and the solution was saturated three times with ammonia gas. The solution was concentrated in vacuo and dried under vacuum overnight. The residue was dissolved in NJN-dimethylacetamide dimethyl acetal (24.7 mL, 0.169 mol) and heated to 120 ""C for 5 hours. Additional N,N-dimethylacetamide dimethyl acetal (25 mL, 0.17 mol) was added over the course of the reaction to drive it to completion. The solution was cooled to room temperature, concentrated in vacuo, and dried under vacuum overnight. To a solution of the intermediate in 57 mL dioxane was added hydroxylamine hydrochloride (4.704g, 67.69 mmol), 5N NaOH (13.5 mL, 67.7 mmol), and 70% acetic acid (57 mL). The mixture was stirred at 60 °C for 2 hours, then at 90 °C for 3 hours. The resulting solution was cooled to room temperature, diluted with ethyl acetate, and neutralized with aqueous sodium bicarbonate. The organic extract was washed with aqueous sodium bicarbonate and brine, dried over sodium sulfate, filtered and concentrated under vacuum. The residue was filtered through silica gel eluted with 10% ethyl acetate in hexanes to provide 5-(2-fluoro-6-iodophenyl)-3-methyl-L2.4-c\adiazo!e as orange yellow crystals that gave proton NlvIR spectra consistent with theory and a mass ion (ES+) of 305.06 for M+H+. To a solution o: (S)-(-)-2-methyl-2-propanesuIfinamide (20.20 g, 0.167 mol) in 350 mL CH2CI2 were added 4-bromo-2-fluorobenzaldehyde (35.53g, 0.1750 mol), pyridinium-p-toIuene5u:fonate (2.09g, 8.33 mmol), and magnesium sulfate (200.6 g, 1.667 mol). The reaction mixture was stirred at room temperature for 48 hours. Additional magnesium sulfate (100.3g , 0.833 mol) was added, and the reaction was stirred 24 hours. The mixture was filtered through celite, washing with CH2CI2 and concentrated in vacuo. The resulting residue was subjected to column chromatography on silica gel eluted with 0-10% ethyl acetate in hexanes to afford N-[(lE)-(4-bromo-2-fluorophenyl)methylidene]-2-methylpropane-2-sulfinamide as a white solid that gave proton NMR spectra consistent with theory and a mass ion (ES+) of 308.09 forM+H+(SlBr). To a solution of N-[(iE)-(4-bromo-2-fluorophenyl)methylidene]-2-methylpropane-2-sulfinamide (32.65g, 0.1066 mol) in 550 mL CH2CI2 at -48 °C was added methylmagnesium chioride (3.0 M solution is ether, 53.31 mL, 0.1599 mol) dropwise. The reaction was quenched with aqueous ammonium chloride and the aqueous layer was extracted with methylene chloride. The combined organics were dried over Na2S04, filtered and concentrated under vacuum. The resulting residue was subjected to column chromatography on silica gel eluted with 10-50% ethyl acetate in hexanes to afford N-[(lR)-l-(4-bromo-2-fluorophenyl)ethyl]-2-methyl-propane-2-sulfinainide as a white solid that gave proton NMR spectra consistent with theory and a mass ion (ES+) of 324,14 for M+H+(8lBr). To a solution of N-[(lR)-l-(4-bromo-2-fluorophenyl)ethyl]-2-methyl-propane-2-sulfinamide (26.29g, 81.58 mmol) in 40 mL methanol was added HCl/ dioxane (4M, 40,8 mL, 0.163 mol) solution. The reaction mixture was concentrated in vacuo, and ether was added. The white solid was collected, washing with cold ether, and dried under vacuum to yield (lR)-l-(4-bromo-2-fluorophenyl)ethan-aminium chloride that gave proton NMR spectra consistent with theory. To a solution of the above (lR)-l-(4-bromo-2-fluorophenyl)-ethan-aminium chloride (14.24g, 55.95 mmol) in 300 mL CH2CI2 at 0 °C was added di-ten- butyl dicarbonate (17.9Sg, 82.40 mmol) and triethylamine (8.256 g, 81.58 mmol). The solution was washed with water and brine, dried over Na2S04, filtered and concentrated under vacuum to provide crude (lR)-l-(4-bromo-2-fluorophenyl)ethan' aminium chloride as a \\hite solid that gave proton NMR spectra consistent with theory. A mixture of i lR)-l-(4-bromo-2-f]uorophenyr]ethanamimum chloride (26.42g, 83.03 mmol), bi5(pinacolato)diboron (31.63g, 0.1246 mol), potassium acetate (24.45 g, 0.2491 mol), and [l,1r-bis(diphenylphosphino)ferrocene]- palladium(D) dichloride (0.265 g, 0.362 mmol) in SO mL DMSO was heated to 90 °C under N2 for 3 hours. The mixture was then cooled to room temperature and partitioned between ethyl acetate and water. The organic extract was washed with water and brine, dried over Na2S04, filtered and concentrated under vacuum. The residue was subjected to silica gel chromatography eluted with 0-10% ethyl acetate in hexanes to provide tert-butyl (lR)-l-[2-fluoro-4-(4,4,5,5-tetramethyl-l,3,2-dioxa- borolan-2-yl)phenyl]ethylcarbonate as a beige solid that gave proton NMR spectra - consistent with theory. A mixture of 5-(2-fluoro-6-iodophenyI)-3'methyl-l,2,4-oxadiazole (1.50g, 4.93 mmol), tert-butyl (lR)-l-[2-fluoro-4-(4,4,5,5-tetramethyl-l,3,2-dioxa- borolan-2-yl)phenyl]ethylcarbonate (1.80 g, 4.93 mmol), potassium carbonate (1.70 g, 12.3 mmol), tri-o-tolyiphosphine (0.060 g, 0.20 mmol), and palladium acetate (0.011 g, 0.05 mmol) in 25 mL of THF and 4 mL of water was heated in a sealed flask at 100°C overnight. The mixture was then cooled and concentrated in vacuo. The resulting residue was dissolved in ethyl acetate, washed with water and brine, dried over Na2S04, filtered and concentrated under vacuum. The residue was subjected to silica gel chromatography eluted with 0-10% ethyl acetate and hexane to provide tert-butyl (lR)-l-[33'-ciifluoro-2'-(3-methyl-l,2,4-oxadia2ol-5-yl)-l,r-biphenyM-yl]-ethylcarbamate as a yellow oil that gave proton NMR spectra consistent with theory. The product was dissolved in ethyl acetate and saturated with HCI gas. The solution was concentrated in vacuo and azeotroped 3 times with toluene to provide (1R)-1- [3,3-difluoro-2'-(3-methyI-l,2,4-oxadia2ol-5-yI)-l,r-biphenyl-4-yI]ethanamihium chloride. A solution of (lR)-l-[3,3'-difluoro-2'-(3-methyl-1,2,4-oxadia2ol-5-yl)-l,r-biphenyl-4-yl]ethanaminiuni chloride (O.SOOg, 1.42 mmol), Boc-l-aminocyclo-propane-l-carboxylic acid (0.3C0g, 1.49 mmol), l-ethyl-(3-dimethylaminopropyl)-carbodiimide hydrochloride (0.545 g, 2.84 mmol), l-hydroxy-7-azabenzotriazole (0.010 g, 0.15 mmol), and tnethylamine (0.863 g, 8.53 mmoi) in 10 mL CH2CI2 was stirred at room temperature overnight. The solution was washed with aqueous sodium bicarbonate and brine, dnec over Na2S04, filtered and concentrated. The residue was subjected to silica gel chrorr. Jtography eluted with 10-40% ethyl acetate in hexanes to provide tert-butyl 1-[({(1R;)-1-[3,3-difluoro-2H3-methyl-l,2.4-oxadiazol-5-yl)-l,r-bipheny]-4-yI]ethyl}amino>carbo^yl]cyclop^opylcarbamate as a white solid that gave proton NMR spectra consistent with theory. The product was dissolved in ethyl acetate and saturated with KCl sas. The solution was concentrated in vacuo and azeotroped 3 times with toluene to provide l-[(((lR)-l-[3,3-difIuoro-2-(3-methyl-l,2,4-o\adiazol-5-yl)-l,l,'biphenyl-4-yl]ethyl}amino)carbonyl]-cyclopropanaminium chloride that gave a mass ion (ES+) of 399.21 for M+H+. To a solution of the above compound (0,290 g, 0.667 mmol) in 5 mL CH2CI2 at 0 °C was added trlethylamine (0.135 g, 1.33 mmol) and trifluoroacetic anhydride (0,14 g, 0.67 mmoi). The solution was diluted with addition CH2CI2 and washed with aqueous sodium bicarbonate and brine, dried overNa2S04, filtered and concentrated. The residue was subjected to silica gel chromatography eluted with 10- 40% ethyl acetate in hexanes :o provide the title compound that gave proton NMR spectra consistent with theory and a mass ion (ES+) of 495.22 for M+H+: 1H NMR (300 MHz, MeOH-d4) 5 9.71 (s, 1H), 8.29 (d, / = 8.3 Hz, 1H), 7.77-7.70 (m, 1H), 7.41-7.30 (m, 3H), 6.95 (s, IK), 6.93-6.91 (m, 1H), 5.32-5.22 (m, 1H), 2.36 (s, 3H), 1.5M.45 (m, 5H), 1.15-1.01 (m, 2H). To a solution of n-BuLi (2.5M in hexanes, 41.8niL, 0.104 mol) in 400 mL THF at -78 °C was added 2,2,6,6-tetramethylpiperidine (14.76 g, 0.1045 mol) dropvvise followed by 3-bromochIorobenzene (20.00g, 0,1045 mol) dropwise. The mixture was stirred at -7S °C for 2h, then quenched with dry ice and warmed to room temperature. The solution was concentrated in vacuo and the resulting residue was dissolved in water and washed with ether. To the aqueous fraction was added IN HCl to pH = 2, and the product was extracted with CH2CI2. The combined organics were dried over Na2S04, filtered and concentrated under vacuum to provide 2-bromo-6- chlorobenzoic acid that gave proton NMR spectra consistent with theory. To a solution of 2-bromo-6-chlorobenzoic acid (17.7g, 75.2 ramol) in methanol (200 mL) at 0° was added (trimethylsilyl)diazomethane (2M in hexanes, 100 mL, 0.200 mol). The solution was stirred at 0 °C for 1.5 hours, then warmed to room temperature and washed with aqueous sodium bicarbonate and brine, dried over Na2S04, filtered and concentrated. The residue was subjected to silica gel chromatography eluted with 0-5% ethyl acetate in hexanes to provide methyl 2-bromo-6-chlorobenzoate as a pale yellow oil that gave proton NMR spectra consistent with theory. A mixture of methyl 2-bromo-6-chlorobenzoate (2.25g, 9.03 mmol), tert-butyl(lR)-l-[2-f]uoro-4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)phenyI]-ethylcarbonate (see Example 11, 3.00 g, 8.21 mmol), potassium carbonate (2.84 g, 20.5 mmol), tri-o-tolylphosphine (0.10 g, 0.33 mmol), and palladium acetate (0.018 g, 0.08 ramol) in 40 mL of THF and 4 mL of water was heated in a sealed flask at 100°C for 4h. The mixture was then cooled and concentrated in vacuo. The resulting residue was dissolved in ethyl acetate, washed with water and brine, dried over Na2S04, filtered and concentrated under vacuum. The residue was subjected to silica gel chromatography eluted with 0-10% ethyl acetate and hexane to provide methyl 4'-{(lR)-l-[(tert-butoxycarbonyl)amino]ethyl}-3-chloro-3-fluoro-I,r-biphenyl-2-carboxylate that gave proton NMR spectra consistent with theory. The product was dissolved in ethyl acetate and saturated with HCl gas. The solution was concentrated in vacuo and azeotroped 3x with toluene to provide (iR)-l-[3'-chloro-3-fluoro-2'-(methoxycarbonyI)-l,r-biphenyI-4-yl]ethanaminium chloride. A solution of the above compound (i.OOg, 2.91 mmol), boc-1-amino-cyclopropane-1-carboxylic ac:d (0.614g, 3.05 mmol), l-ethyl-(3-dimethylamino-propyl)carbodiimide hydrochloride (l.Ug, 5.81 mmol), l-hydroxy-7-azabenzotriazole (0.010 g, 0.15 mmol), and trie±ylamine (1.76 g, 17.4 mmol) in 20 mL CH2CI2 was stilted at room temperature overnight. The solution was washed with aqueous sodium bicarbonate and brine, dned over Na2S04, filtered and concentrated. The residue was subjected to silica gel chromatography eluted with 10-40% ethyl acetate in hexanes to provide methyl 4-((lR)-i-[({ l-[(tert-buto\ycarbonyl)aminojcyclopropyl)carbonyl)- amino]ethyl}-3-chloro-3'-fiuoro-l,1-biphenyl-2-carboxylate as a white solid that gave proton N?vIR spectra consistent with theory. The product was dissolved in ethyl acetate and saturated with HCl gas. The solution was concentrated in vacuo and azeotroped three times wiih toluene to provide l-[({(lR)-l-[3'-chloro-3-fluoro-2- (methoxycarbonyl)-l,r-biphenyl-4-yl]ethyl}amino)carbonyI]cyclopropanaminium chloride that gave a mass ion (ES+) of 391.21 for M+H+. To a solution of the above compound (0.300 g, 0.702 mmol) in 5 mL CH2CI2 at 0 °C was added triethylamine (0.142 g, 1.40 mmol) and trifluoroacetic anhydride (0.147 g, 0.70 mmol). The solution was diluted with addition CHoCh and washed with aqueous sodium bicarbonate and brine, dried over Na2S04, filtered and concentrated. The residue was subjected to silica gel chromatography eluted with 10-40% ethyl acetate in hexanes to provide the title compound that gave proton NMR spectra consistent with theory and a mass ion (ES+) of 487.22 for M+H+: iH NMR (300 MHz, MeOH-d4) 5 7.51-7.33 (m, 4H), 7.17-7.07 (m, 2H), 5.31 (q, 7 = 7.1 Hz, 1H), 3.69 (s, 3H), 1.52-1.49 (m, 5H), 1.27-1.03 (m, 2H). EXAMPLE 6 N-{(lR)-l-[33'-difluoro-2H2-methyl-2H-tetrazol-5-yl)-l,r-biphenyl-4-yl]ethyl}-l-[(trifluoroacetyl)amino]cyclopropanecarboxanude A solution of 2-fluoro-6-iociobenzonitrile (17.82g, 72.15 mmol) and azidotrinnethylcin (15.OOg, 72.88 mmol) in 150 mL toluene was heated to 125 °C for 72 hours. The solution u-as cooled to room temperature and partitioned between ethyl acetate and 0.5 N HCl. The organic extract was washed with water and brine, dried overNa2S04, filtered and concentrated under vacuum to provide 5-(2-fluoro-6-iodo- phenyl)-lH-tetrazole that gave proton NMR spectra consistent with theory and a mass ion (ES+) of 291.01 for M+H+. A mixture of 5-(2-fluoro-6-iodophenyI)-lH-tetra2ole (23.48g, 80.97 mmol), potassium carbonate (16.79g, 0.121 mol), and iodomethane (16.09g, 0.113 mol) in 25 mL DIVEF was stirred at room temperature for 3 hours. The mixture was partitioned between ethyl acetate and water, and the organic extract was washed with water and brine, dried over Na2S04, filtered and concentrated under vacuum. The residue was subjected to silica gel chromatography eluted with 0-10% ethyl acetate in hexanes to provide 5-(2-fluoro-6-iodophenyl)-2-methyl-2H-tetrazole that gave proton NMR spectra consistent with theory and a mass ion (ES+) of 305.06 for M+H+. To a solution of 2-fluoro-4-bromo-benzaldehyde (20.0 g, 98.5 mmol) in 500 mL of THF at 0 °C was added slowly a solution methyllithium (1.4 M in Et20, 70.3 mL). After one hour the reaction was carefully quenched with saturated aqueous ammonium chloride, extracted with diethyl ether, washed with saturated NaCl, dried over MgS04^ and concentrated. A solution of the crude alcohol in 200 mL of CH2CI2 and TEA (14.0 "mL, 100 mmol) at 0 °C was added methanesulfonyl chloride (7.42 mL, 95.9 mmol) over a period of five minutes. After and overnight reaction period, the reaction was extracted with CH2CI2 , washed with NaHC03, saturated NaCI, dried over MgS04 , and concentrated. A solution of the crude mesylate in 50 mL of DMF was treated with sodium azide (12.5 g, 191 mmol) overnight. The reaction was extracted with EtOAc , washed with NaHCOs, saturated NaCl/dried over MgS04 . and concentrated. To a solution of the crude azide in 100 mL of THF was triethylphosphine (1 M in THF, 90.1 mL) over a period of 30 minutes. After one hour, 100 mL of 1 N HCl was added and the mixture was heated overnight. The mixture was cooled and washed with Et20. The aqueous layer was made basic by the addition of potassium carbonate, extracted with Et20, dried over MgS04 , and concentrated. A solution of the crude amine (9.0 g, 41.2 mmol) in 50 mL of CH2CI2 was treated with Boc anhydride (9.00 g, 41.2 mmol). After one hour the mixture was concentrated. A solution of the crude carbamate in 40 mL of DMSO was added potassium acetate (12.1 g, 123.1 mmol), bis(pinacolato)diboron (11.5 g, 45.4 mmol), and dichloro[l,r-bis(diphenylphosphino)ferrocene]pa!ladiuni (H) dichloromethane adduct (1.4 g, 1.8 mmol). The resulting mixture was heated at 80 °C for 16 hours. After cooling to room temperature, the mixture was partitioned between water and EtOAc. The organic extrac: was washed with brine, dried over MgS04, filtered and concentrated under vacuum. The residue was subjected to silica gel chromatography eluted with 20% ethyl acetace in hexanes to provide tert-butyl i-[2-fluoro-4-(4,4,5,5- tetramethyl-l,3,2-dioxaboroian-2-yl)phenyl]ethylcarbamate as a tacky solid that gave a proton NMR consistent with theory. A mixture of 5-(2-fluoro-6-iodophenyl)-2-methyI-2H-tetra2ole (1.49g, 4.89 mmol), tert-butyl l-[2-riuoro-4-(4,4,5,5-tetramethyI-l,3,2-dioxaborolan-2-yl)- phenyl]ethylcarbamate (l-70g, 4.65 mmol), potassium carbonate (1.61 g, 11.6 mmol), tri-o-tolylphosphine (0.057 g, 0.19 mmol), and palladium acetate (0.010 g, 0.05 mmol) in 20 mL of THF and 4 mL of water was heated in a sealed flask at 100 °C overnight. The mixture was then cooled and concentrated in vacuo. The resulting residue was dissolved in ethyl acetate, washed with water and brine, dried over Na2S04, filtered and concentrated under vacuum. The residue was subjected to silica gel chromatography eluted with 0-20% ethyl acetate and hexane to provide tert-butyl l-[3,3'-difluoro-2'-(2-methyI-2H-tetrazol-5-yl)-l,1-biphenyl-4-yl]ethyIcarbamate as a yellow oil that gave proton NMR spectra consistent with theory. The product was dissolved in ethyl acetate and saturated with HCl gas. The solution was concentrated in vacuo and azeotroped 3x with toluene to provide l-[3,3'-difluoro-2'-(2-methyl-2H-tetra2ol-5-yl)-l, r-biphenyl-4-yI]ethanaminium chloride. A solution of l-[3,3'-difluoro-2'-(2-methyl-2H-tetra2ol-5-yl)-l,r" biphenyl-4-yl]ethanaminium chloride (0.670g, 1.89 mmol), Boc-1-aminocyclo-propane-1-carboxylic acid (0.419g, 2.08 mmol), l-ethyl-(3-dimethylaminopfopyl)-carbodiimide carboxylic acid (0.726g, 3.79 mmol), l-hydroxy-T-azabenzotriazoIe (O.OlOg, 0.15 mmol), and triethylamine (1.15g, 11.4 mmol) in 10 mL CH2CI2 was stirred at room temperature for 48 hours. The solution was washed with aqueous sodium bicarbonate and brine, dried over Na2S04, filtered and concentrated. The residue was subjected to silica gel chromatography eluted with 10-50% ethyl acetate in hexanes to provide tert-butyl !-[({l-[3,3'-difluoro-2'-(2-methyl-2H-tetra2ol-5-yl)-l,r-biphenyl-4-yl]ethyl}amino)carbonyl]cyclopropylcarbamate that gave proton NMR spectra consistent with theory and a mass ion (ES+) of 499.7 for M+H+. The enantiomers were resolved at this point on a Chiralcel OD column 10% isopropanol/ hexane (containing 0.1% TFA). The R enantiomer elated first and was used for the remainder of the synthesis. The above product was dissolved in ethyl acetate and saturated with HCl gas. The solution was concentrated in vacuo and azeotrooed 3x with toluene to provide (lR)l-[((l-[3,3'-d!fluoro-2-(2-methyl-2H-tetrazol-5-yl)-l,l-biphenyl-4-yl]-ethyl }amino)carbonyl]cyclcpropanaminium chloride that gave a mass ion (ES-f) of 399.6 for M-+H+. To a solution of the above compound (0.105g, 0.241 mmol) in 2 mL CH2CI2 at 0 °C was added triethylamine (0.049g, 0.48 mmol) and trifluoroacetic anhydride (0.05Ig, 0.24 mmol). The solution was diluted with additional CH2CI2 and washed with aqueous sodium bicarbonate and brine, dried over Na2S04, filtered and concentrated. The residue was subjected to silica gel chromatography eluted with 10-40% ethyl acetate in hexanes to provide the title compound that gave proton NTVIR spectra consistent with theory and a mass ion (ES+) of 495.32 for M+H+: 1H NMR (300 MHz, MeOH-d4) 5 7.6S-7.61 (m, 1H), 7.34-7.21 (m, 3H), 6.89-6.83 (m, 2H), 5.23 (d. / = 7.1 Hz, 1H), 4.86 (s, 3H), 1.49 (d, J = 3.7 Hz, 2H), 1.44 (d, 7 = 7.I Hz, 3H), 1.18-101(m,2H). The following compounds in Table 2 were prepared by methods analogous to those described in Example 1. The following compounds in Table 4 were prepared by methods analogous to those described in Example 1. The following compounds in Table 5 were prepared by methods analogous to those described in Example 2. The following compounds in Table 7 were prepared by methods analogous to those described in Example 1. The following compounds in Table 9 were prepared by methods analogous to those described in Example 3, using the commercially available l-[(tert-butoxycarbonyl)amino]cyclobutanecarboxylic acid instead of l-[(tert-butoxy-carbonyl)amino]cyclopropanecarboxylic acid. The following compounds in Table 10 were prepared by methods analogous to those described in Example 1, using the commercially available l-[(tert-butoxycarbonyl)amino]cyclobutanecarboxylic acid instead of l-[(tert-butoxy-carbonyl)amino]cyclopropanecarboxylic acid. New Claims WHAT IS CLAIMED 15: I. A compound having the formula 1(3) and pharmaceutically Acceptable salts thereof: wherein m is 0 or 1. R6a 2-niethy!-2H-tetrazol-5-yL 3-melhyl-l,2,4-oxadiazol-5-yl. C02R or C(0)NHOR. harem Ra is alkyl; R6b is hydrogen, fluorine or chlorine; R3b is alky is selected from alkyl optionally substituted with 1 to 5 halogen atoms or a cyano group, C3-6 isoxazolyl, pyrimidinyl and pyridines (and N-oxide thereof) optionally substituted with halogen or trifluoromethyl; and Ram is hydrogen or fluorine. 3. A compound of Claim 1 selected from; methyl 3-fluoro-4'-{(lR)-I-[({l-[(3,3,3-trif]uoropropanoyl)amino]cyclopropyl}-carbonyl)amino]ethyl} -1,1 '-biphenyl-2-carboxylate, 3-fluoro-N-methoxy-4'-{(lR)-l-[({l-[(3,33-trifluoropropanoyl)amino.]cycIopropyl}- carbonyl)amino]ethyl} -1,1- '-biphenyl-2carboxamide, methyl 3-f]uoro-4'-{1 -methyl-1 -[({1 -[(trifluoroacer\1)amino]cyclopropyl}- carbonyl)amino]ethyl } -1,1 '-biphenyl-2-carboxylate, N-{(lR)-l-[3,3'-difluoro-2X3-methyl4,2,4.oxadiazoI-5-yl)-l,r.biphenyl-4- yl]ethyl}-I-[(trifluoroacetyl)amino]cyclopropanecarboxamide, methyl 3-chIoro-3'-fluoro-4*- {(1R)-1 -[({1 -[(trifluoroacetyl)amino]cyclopropyl} - carbonyl)amino]ethyl}-1,1 '-biphenyl-2-carboxylate, and N-{(lR)-l-[3,3'-difluoro-2X2-methyl-2H4etrazolo'-yl)-iphenyI-4-yI]eth[(trifluoroacetyl)amino]cyclopropanecarboxamide; and pharmaceutically acceptable salts thereof 4. The compound methyl 3-chloro-3'-fluoro-4'-{(lR)-l-[({l-[(trifluoroacetyl)amino]cyclopropyl} carbonyl)amino]ethyl} -1,1 '-biphenyl-2-carboxylate. 5. A pharmaceutical composition comprising a therapeutically effective amount of a compound of Claim 1 and pharmaceutically acceptable recipients. 6. Use of a compound of Claim 1 or a pharmaceuiically acceptable salt thereof in the manufacture of a medicament for the treatment or prevention of pain and inflammation. 7. Use of a comopound of Claim 4 for the manufacture of a medicament for the treatment and prevention of pain and inflammation. 8. The use of Claim 6 wherein said pain is postherpetic neuralgia, osteoarthrititis pain, post- or perioperative pain or dental pain. 9. The use of Claim 7 wherein said pain is postherpetic neuralgia, osteoarthrititis pain, post- or perioperative pain or dental pain. 10. A compound having the formula 1(3) and pharmaceutically acceptable salts thereof, substantially as herein described herein and extemporized.

Full Text

N-BIPKENYLMETHYL AMINOCYCLOALKANECARBOXAMIDE
DERIVATES VIITH A SU3STITUENT ON
THE METHYL USEFUL AS BRADYKIMIN A^TTAGONISTS
BACKGROUND OF THE INVENTION
This invention is directed to aminocycloalkanecarboxamide compounds. In particular, this invention is directed to aniinocycloalkanecarboxamide compounds that are bradykinin antagonists or inverse agonists.
Bradyldnin ("BK") is a kinin which plays an important role in the pathophysiological processes accompanying acute and chronic pain and inflammation. Bradykinin (BK), like other kinins, is an autacoid peptide produced by the catalytic action of kallikrein enzymes on plasma and tissue precursors termed kininogens. The biological actions of BK are mediated by at least two major G-protein-coupled BK receptors termed Bl and B2. It is generally believed that B2 receptors, but not Bl receptors, are expressed in normal tissues and that inflammation, tissue damage or bacterial infection can rapidly induce Bl receptor expression. This makes the Bl receptor a particularly attractive drug target. The putative role of kinins, and specifically BK, in the managem.ent of pain and inflammation has provided the impetus for developing potent and selective BK antagonists. In recent years, this effort has been heightened with the expectation that useful therapeutic agents with analgesic and anti-inflammatory properties would provide relief from maladies mediated through a BK receptor pathway (see e.g., M.G. Bock and J, Longmore, Current Opinion in Chem. Biol., 4:401-406(2000)). Accordingly, there is a need for novel compounds that are effective in blocking or reversing activation of bradykinin receptors. Such compounds would be useful in the management of pain and inflammation, as well as in the treatment or prevention of diseases and disorders mediated by bradykinin; further, such compounds are also useful as research tools {in vivo and in vitro),
Canadian Published Application No. 2,050,769 discloses compounds of the formula:

which are intermediates in the preparation of angiotensin II antagonists.
SUMMARY OF THE INVENTION
The present invention provides biphenyl cycloalkanecarboxamide derivatives which are bradykir.in antagonists or inverse agonists, pharmaceutical compositions containing such compounds, and methods of using them as therapeutic agents.
DET.AILED DESCRIPTION OF THE INVENTION
The present invention provides compounds of formula I and pharmaceutically acceptable salts thereof:

wherein
Rl and R2 are independently selected from
(1) hydrogen and
(2) C1-4alkyl;
R3a is selected from
(1) hydrogen and
(2) C1-4 alkyl optionally substituted with 1 to 5 halogen atoms;
R3b is C1-4 alkyl optionally substituted with 1 to 5 halogen atoms; R4a and R4b are independently selected form
(1) hydrogen.
(2) halogen, and

(3) C1-4 alkyl optionally substituted with 1 to 4 groups selected from halogen, ORa, OC(0)Ra. S(0)kRd, OS(0)2Rd, and NRIR2, or
R4a and R4b together with the carbon atom to which they are both attached form an exo-cyclic methylene optionaly substituted with 1 to 2 groups selected from C1-4
alkyl optionally substituted wi:n 1-5 halogens and C1-4 alkyloxy:
R5 is selected from
(1) C1-6 alkyl optionally substituted with 1 to 5 groups
independently selected from halogen, nitro, cyano, ORa, SRa, CORa, S02Rd, C02Ra. OC(0)Ra, NRbRc, NRbC(0)Ra, NRbC(0)2Ra, C(0)NRbRC, C3-8
cycloalkyl,
(2) C3-8 cyc'-oalkyl optionally substituted with 1 to 5 groups
independently selected from halogen, nitro, cyano and phenyl,
(3) C3-6 alkynyl,
(4) C2-6 alkenyl optionally substituted with hydroxyethyl,
(5) (CH2)k-aryl optionally substituted with 1 to 3 groups independently selected from halogen, nitro, cyano, ORa, SRa, C(0)2Ra, C1-4 alkyl and C1-3 haloalkyl, wherein aryl is selected from phenyl, 3,4-methyienedioxyphenyI
and naphthyl;
(6) (CH2)k-beterocycle optionally substituted with 1 to 3 groups
independently selected from halogen, nitro, cyano, ORa, SRa, C1-4 alkyl and Ci_3
haloalkyl wherein said heterocycle is selected from (a) a 5-membered heteroaromatic ring having a ring heteroatom selected from N, O and S, and optionally having up to 3 addidonal ring nitrogen atoms wherein said ring is optionally benzo-fused; (b) a 6-membered heteroaromatic ring containing from 1 to 3 ring nitrogen atoms and N-oxides thereof, wherein said ring is optionally benzo-fused; and (c) a 5- or 6-membered non-aromatic heterocyclic ring selected from tetrahydrofuranyl, "5-oxo-tetrahydrofuranyl, 2-oxo-2H-pyranyl, 6-oxo-l,6-dihydropyridazinyI,
(7) C(0)2Ra, and
(8) C(0)NRbRC; R6a is selected from

(1) C1-8 alkyl optionally substituted with 1-5 groups independently selected from halogen, nitro, cyano, CORa, C02Ra, C(0)NRbRc, ORa, OC(0)Ra, SRa, S02Rd, S(0)Rd, NRbRc, N-Rbc(0)Ra, NRbS02R^, NRbc02Ra,
(2) C3-8 cycloalkyl.

(3) C2-8 alkenyl optionally substituted with C02Ra,
(4) halogen,
(5) cyano,
(6) nitro,
(7) NRbRc,
(8) NRbC(0)Ra,
(9) NRbC02Ra,
(10) NRbc(0)NRbRc,
(11) NRbC(0)NRbC02Ra
(12) NRbS02Rd,
(13) CC)2Ra,
(14) CORa,
(15) C(0)NRbRc,
(16) C(0)NHORa,
(17) C(=NORa)Ra
(18) C(=NORa)NRbRc,
(19) ORa
(20) OC(0)Ra,
(21) S(0)kRd,
(22) S02iNfRbRc, and
(23) optionally substituted heterocycle where the heterocycle is a 5-
membered heteroaromatic ring having a ring heteroatom selected from N, O and S,
and optionally having up to 3 additional ring nitrogen atonis, 4,5-dihydro-oxazolyl
and 4,5-dihydro-l,2,4-oxadiazoIyl, and wherein said substituent is 1 to 3 groups independently selected from C1-4 alkyl optionally substituted with 1 to 5 Kalogen
atoms, ORa or OC(0)Ra.
R6b and R6C are independently selected from
(1) hydrogen, and
(2) a group from R6a; with the proviso that not more than one of R6a, R6b, and R6C is a heterocycle;
R7a and R7b are independently s-elected from
(1) hydrogen,
(2) halogen,
(3) cyano.

(4) nitro,
(5) ORa,
(6) C02Ra
(7) C(0)NRbRc,
(8) C1-4 alky! optionally substituted with i to 5 halogen atoms,
(9) NRbRc, and
(10) S(0)kRd;
Ra is selected from
(1) hydrogen,
(2) C1-4 alky! optionally substituted with i to 5 halogen atoms,
(3) phenyl optionally substituted with 1 to 3 groups independently selected from halogen, cyano, nitro, OH, C1-4 alkyloxy, C3-6 cycloalkyl and C1-4
alkyl optionally substituted with 1 to 5 halogen atoms,
(4) C3-6 cycloalkyl, and
(5) pyridyl optionally substituted with 1 to 3 groups independently selected from halogen and C1-4 alkyl;
Rb and Rc are independently selected from
(1) hydrogen,
(2) C1-4 alkyl optionally substituted with I to 5 groups
independently selected from halogen , amino, mono-C1-4alkylamino, di-C1-4alkylamino, and S02Rd,
(3) (CH2)k-phenyl optionally substituted with 1 to 3 groups
selected from halogen, cyano, nitro, OH, C1-4 alkyloxy. C3.6 cycloalkyl and C1-4
alkyl optionally substituted with 1 to 5 halogen atoms, and
(4) C3.6 cycloalkyl, or
Rb and Rc together with the nitrogen atom to which they are attached form a*4-, 5-, or
6-membered ring optionally containing an additional heteroatom selected from N, O,
and S; or
Rb and Rc together with the nitrogen atom to which they are attached form a cyclic
imide;
Rd is selected from
(1) C1-4 alkyl optionally substituted with 1 to 5 halogen atoms,
(2) C1-4 alkyloxy, and

(3) phenyl optionally substituted with 1 to 3 groups selected from halogen, cyano, nitro, OH, C1-4 alkyloxy, C3-6 cycloalkyl and C1-4 alkyl optionally
substituted with I to 5 halogen atoms; k is 0,1 or 2; and misO or 1.
For compounds of fonnula I, examples of Rl and R2 include hydrogen, methyl, ethyl, n-propyl, isopropyl, n-butyl, t-butyl, isobutyl and sec-butyl. In one embodiment of formula I are compounds wherein R1 and R2 are each hydrogen.
Examples of R3a and R3b for compounds of formula I include
hydrogen, methyl, ethyl, n-propyl, isopropyl, n-butyl, t-butyl, isobutyl, sec-butyl,
chloromethyl, fluromethyl, trifluoromethyl, 2,2,2-trifluoroethyl, 2,2-difluoroethyl,
1,1,2,2.2-pentafluoroethyl, and the like. In one embodiment of formula I are compounds wherein one of R3a is hydrogen and R3b is C1.4 alkyl. In one subset
thereof R3b is methyl.
Examples R4a and R4b for compounds of formula I include hydrogen, methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, isobutyl, t-butyl, chlorine, fluorine, bromine, chloromethyl, 1-chloroethyl, hydroxymethyl, 2-methoxyethyl, ethoxymethyl, acetyloxymethyl, methylthiomethyl, aminomethyl, methylamino-methyl, (dimethylamino)methyl, (methylsulfonyl)oxyraethyl, and the like; or R4a and R4b on the same carbon atom taken together represent methylene. In one embodiment of formula I are compounds wherein one of R4a and R4b is hydrogen and the other is selected from hydrogen, halogen and C1-4 alkyl optionally substituted with a group selected from halogen, ORa, OC(0)Ra, S(0)kRd, OS(0)2Rd, and NRiR2, or R4a and R4b together with the carbon atom to which they are both-attached fonn an exo-cyclic methylene. In one subset thereof R4a and R4b are each hydrogen; in another subset R4a is hydrogen and R^b is selected from CH2-halogen, CH2-0Ra, CH2-0C(0)Ra CH2-S(0)kRd CH2-OS(0)2Rd and CH2-NR1R2; in a further subset R4a is hydrogen and R4b is selected from hydroxymethyl, acetyloxymethyl, chloromethyl, (methanesulfonyl)oxymethyl, (methylthio)methyl and (dimethylamino)methyl.
Examples of R5 for compounds of formula I include methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyL isobutyl, t-butyl, 1-ethylpropyl, 2,2-dimethyl-propyl, broraomethyl, chloromethyl, dichloromethyl, difluoromethyl, trifluoromethyl, chlorodifluoromethyl, cyanomethyl, aminomethyl, acetylaminomethyl, dimethyl-

aminomethyl, hydroxymethyl, methoxymethyl, ethoxymethyl, methylsulfonylmethyl,
phenylthiomethyl, phenoxymethyl, 1-aminoethyl, 1-acetylaminomethyl, 1-imidazolyl-
methyl, t-butoxycarbonylaminomethyl, 3-pyridylcarbonylmethyl, 1-chloroethyl, 1,1-
dichloroethyl, 2,2,2-trifluoroethyI, pentafluoroethyl, 2-methoxyethyl, 2-phenylethy!,
2-cyclopentylethyl, 2-carboxyethyl, 2-methoxy-2-oxoethyl, 2-nitroethyI^ 1,1-difluoro-
i-hydroxypropyl, 1-hydroxypropyI, 2-oxopropyl, 3-metho\y-3-oxopropyl, 1-cyano-
cyclopropyl, cyclopropyh cyclopentyl, 2-phenylcyclopropy!, allyl, ethenyl, 1-(1-
hydroxyethyi)vinyl, 3-burynyl, propargyl, phenyl, benzyl, 3,5-bis(trifluoromethyl)-
phenyl, 2,4-difluordphenyl, 4-nneihylphenyl, 3,4-dimethoxybenzyl, 3,4-dimethoxy-
phenyl, 4-cyanophenyl, 3-nitrophenyl, 2-naphthyl, 3,4-methyIenedioxyphenyl, 3-
cyanophenyl, 2-cyanophenyl, 3-fiuorophenyl, 3-methoxyphenyl, 3-chIorophenyl, 3,4-
dichlorophenyl, 3,5-dimethoxyphenyl, 3-trifluoromethylphenyl, 3-methylphenyl, 3,5-
dichlorophenyl, 2-hydroxyphenyl, 3-hydroxyphenyl, 3'nitro-5-(trifluoromethyl)-
phenyl, 5-isoxazolyI, 2-benzothienyl, 2-thienylmethyU 3-pyridyl, 4-pyridyl, 2-furyl, 3-
furyl, 2-thienyl, 3-thienyI, 5-methyl-3-isoxazolyl, 3-tetrahydrofuranyl, 4-methyl-l,2,5-
oxadiazol-3-yl, 5-carboxy-3-pyridyI, 6-hydroxy-2-pyridyl, 5-hydroxy-3-pyridyl, 2-
hydroxy-3-pyridyU 2-methyoxy-3-pyridyl, 6-chloro-2-pyridyl, 2-chloro-3-pyridyl, 5-
chloro-3-pyridyl, 5-fIuoro-3-pyridyl, 5-bromo-3-pyridyl, 5-methyl-3-pyridyl, 3-
(trifluoromethyl)-4-pyridyl, 5-(tririuoromethy!)-3-pyridyil, l-methyl-4-pyrazoIyl, 1-
pyrazolylmethyl, l-methyl-2-imidazolyl, l,2,4-triazol-l-ylmethyl,4-thiazo!yl, 5-oxo-
tetrahydrofuran-2-yl, 2-oxo-5-pyranyl, 3-isoxazolyl, 3-pyridazinyl, 5-pyrimidinyl, 4-
pyrimidinyl, l-methyl-5-pyrazoIylm l-methyl-3-pyrazolyl, 5-thiazolyl, 5-methyl-1-
pyrazoIyImethyI/(3-methyl-l,2,4-toa2ol-5-yl)methyl, 2-(l,2,4-triazol-l-yl)rthyl,5-
raethyM-thiazolyl, 2-quinoxaIinyl, methoxycarbonyl, aminocarbonyl, methylaraino-
carbonyl, dimethylaminocarbonyl, 2-(dimethylamino)ethyIaminocarbonyl, benzyl-
aminocarbonyl, 2-phenethylaniinocarbonyL
In one embodiment of fonnula I are compounds wherein R5 is C1-6
alkyl optionally substituted with 1 to 5 groups independently selected from halogen, nitre, cyano, ORa, SRa, CORa, S02Rd, C02Ra OC(0)Ra NRbRc, NRbC(0)Ra,
NRbC02Ra, C(0)NRbRc, and C3-8 cycloalkyl. In one subset thereof are compounds
wherein R5 is C1-5 alkyl optionally substituted with 1 to 5 groups independently
selected from halogen, nitro, cyano, ORa, SRa, C02Ra and C3-8 cycloalkyl. In a
further subset are compounds wherein R5 is selected from C1.5 alkyl and C1-3 alkyl
substituted with 1 to 5 groups selected from halogen, cyano, hydroxy, C1-4 alkoxy
and C1-4 alkoxycarbonyl. In another further subset R5 is selected from C1-3 alkyl

substituted with 1 to 5 halogen atoms, or a group selected from cyano, hydroxy, C1-4 alkoxy and C1-4 alkoxycarbonyl.
In another embodiment of formula I are compounds wherein R5 is C3-6 cycloalkyl optionally substituted with 1 to 3 groups independently selected from
halogen, nitro, cyano and phenyl. In one subset R5 is C3-6 cycloalkyl optionally substituted with a group selected from cyano and phenyl.
In another embodiment of formula I are compounds wherein R5 is (CH2)k-aryl optionally substituted with 1 to 3 groups independently selected from
halogen, nitro, cyano, ORa, SRa, C1-4 alkyl and C1-3 haloalkyl, wherein aryl is
selected from phenyl, 3,4-methylenedioxyphenyl and naphthyl. In one subset thereof,
R5 is phenyl optionally substituted with 1 to 3 groups independently selected from halogen, trifluoromethyl, nitro, cyano, C1-4 alkoxy and C1-4 alkyl; in a further subset
R5 is phenyl optionally substituted with 1 to 2 groups selected from methyl, trifluoromethyl, halogen, cyano, nitro and methoxy.
In another embodiment of formula I are compounds wherein R5 is (CH2)k-heterocycle optionally substituted with 1 to 2 groups independently selected
from halogen, nitro, cyano, ORa, SRa, C1-4 alkyl and C1-3 haloalkyl wherein said heterocycle is selected from isoxazolyl, thienyl, pyridinyl, benzothienyl, furyl, tetrahydrofuranyl, oxadiazolyl, I-oxidopyridinyl, pyrazolyl, imidazolyl, 1,2,4-triazolyl, thiazolyl, 5-oxotetrahydrofuranyl, 2-oxo-2H-pyranyl, 6-0x0-1,6-dihydro-
pyridazinyl, oxazolyl, pyridazinyl. pyrimidinyl and quinoxalinyl In one subset thereof R5 is selected from isoxazolyl optionally substituted with 1 or 2 Ci^ alkyl,
thienyl, pyridinyl optionally substituted with hydroxy, trifluoromethyl or halogen, benzothienyl, furyl, tetrahydrofuranyl, oxadiazolyl optionally substituted with C1-4
alkyl, 1-oxidopyridinyl optionally substituted with halogen or C1-4 alkyl, pyrazolyl
opdonally substituted with C1-4 alkyl, irnidazolyl optionally substituted with C1-4
alkyl, 1,2,4-triazolyl optionally substituted with C1-4 alkyl, thiazolyl optionally
substituted with C1-4 alkyl, 5-oxotetrahydrofuranyl, 2-oxo-2H-pyranyl, 6-oxo-l,6-
dihydropyridazinyl, oxazolyl, pyridazinyl, pyrimidinyl and quinoxalinyl. In another subset R5 is selected from 5-isoxazolyl, 5-pyrimidinyl, 5-bromo-3-pyridyl and N-oxide thereof, and 5-trifluoromethyl-3-pyridyl
For compounds of formula I examples of R6a include 1-methylethyI, 1-hydroxyethyl, methoxymethyl, 2-oxo-2-methoxyethyl, carboxy, methoxycarbonyl, ethoxycarbonyl, isopropoxycarbonyl, phenoxycarbonyl, cyclopentoxycarbonyl, cyclo-

butoxycarbonyl, cyclopropoxycarbonyl, 2,2,2-trifluoroethoxycarbonyl, 4-trifIuoro-methylphenoxycarbonyl, methoxyaminocarbonyl, methoxycarbonylmethyl, formyl, hydroxy, 3-methyl-l,2,4-oxadiazol-5-yl, 5-methyl-l,2,4-oxadiazol-3-yl, l-methyl-5-tetrazolyl, 2-methyl-5-tetrazoly!, cyano, hydroxy, methoxy, difluoromethoxy, trifluoromethoxy, trifluoromethyl, chloro, fluoro, methylaminosulfonyl, dimethyl-aminosulfonyl, methoxycarbonylamino, ethoxycarbonylamino, 2-fluoroethoxy-carbonylamino, isopropoxycarbonylamino, methylaminocarbonylamino, dimethyl-amino, methylaminocarbonyl, isopropylaminocarbonyl, ethylaminocarbonyl, cyclo-propylaminocarbonyl, cyclobutylaminocarbonyl, dimethylaminocarbonyl and amino-carbonyl; examples for R6b for compounds of formula I include hydrogen, chloro, fluoro, methyl and methoxycarbonyl; example of R6C include hydrogen, chloro, fluoro and methyl; and examples of R^a and R7b include hydrogen, hydroxy, methoxy, methylamino, methylsulfonyl, chloro and fluoro.
In another embodiment of formula I are compounds wherein m is 0.
In another embodiment of formula I are compounds represented by formula 1(1):
wherein m, Rl, R2, R3a R3b, R4a, R4b, R5, R6a, R6b, R6C and R7a have the same definitions as provided under formula L
In a subset of formula 1(1) are compounds wherein R6a is selected from (1) C02Ra (2) C(0)NHORa, (3) cyano, (4) halogen, (5) ORa, (6) C1-8 alkyl optionally substituted with 1-5 halogen atoms, or a group selected from COaRa, C(0)NRbRc and ORa, (7) C(0)NRbRc, (8) NRbC(0)NRbRc, (9) NRbc(0)ORa, and (10) optionally substituted heterocycle where the heterocycle is selected from

oxadiazolyl and tetrazolyl and wherein said substituent is 1 to 3 groups independently selected from C1-4 alkyl optionally substituted with 1 to 5 halogen atoms, ORa or
OC(0)Ra. In a further subset are compounds wherein R6a is selected from C02R^,
C(0)NHORa, methyltetrazolyl, methyloxadiazolyl, NRbC(0)NRbRc, and NRbC(0)ORa
In another subset of formula 1(1) are compounds wherein R6b is selected from hydrogen, halogen and C02Ra. In a further subset R6b is hydrogen or
halogen.
In another subset of formula 1(1) are compounds where R6a is selected from (1) C02Ra, (2) C(0)NHORa, (3) cyano, (4) halogen, (5) ORa, (6) C1-8 alkyl optionally substituted with 1-5 halogen atoms, or a group selected from C02Ra, C(0)NRbRc and ORa, (7) C(0)NRbRc, (8) NRbC(0)NRbRc, (p) NRbc(0)ORa, and (10) optionally substituted heterocycle where the heterocycle is selected from
oxadiazolyl and tetrazolyl and wherein said substituent is 1 to 3 groups independently selected from C1-4 alkyl optionally substituted with 1 to 5 halogen atoms, ORa or
OC(0)Ra; R6b is selected from hydrogen and halogen; and R6C is hydrogen.
In another subset of formula 1(1) are compounds wherein R5 is selected from C1-4 alkyl optionally substituted with 1 to 5 halogen atoms or a cyano
group, C3-6 cycloalkyl, isoxazolyl, pyrimidinyl and pyridinyl (and N-oxide thereof) optionally substituted with halogen.

4

In one embodiment of formula 1(2), R3b is methyl In another embodiment of formula 1(2), R6b is hydrogen or halogen. In one subset R6b is hydrogen; in another subset R^b is fluorine or chlorine.
In another embodiment of formula 1(2), R6a is selected from (1) C02Ra, (2) C(0)NHORa, (3) cyano, (4) halogen, (5) ORa, (6) C1-8 alkyl optionally substituted with 1-5 halogen atoms, or a group selected from C02Ra, C(0)NRbRc and ORa, (7) C(0)NRbRc, (8) XRbc(0)NRbRc, (9) NRbC(0)0Ra, and (10) optionally substituted heterocycle where the heterocycle is selected from oxadiazolyl
and tetrazolyl and wherein said substituent is 1 to 3 groups independently selected from Ci^ alkyl optionally substituted with 1 to 5 halogen atoms, ORa or OC(0)Ra,
In one subset R6a is selected from C02Ra, C(0)NHORa, methyltetrazolyl,
methyloxadiazolyl, NRbC(0)NRbRc, and NRbC(0)0Ra. In a further subset R6a is selected from C02Ra, methyltetrazolyl and methyloxadiazolyl,
In another embodiment of formula 1(2), R6C is hydgrogen or halogen. In one subset R6C is hydgrogen.
In another embodiment R7a is hydrogen or halogen. In one subset R7a is hydrogen. In another subset R7a js fluorine. In yet another subset R6b is hydrogen, fluorine or chlorine, and R7a is hydrogen or fluorine.
In another embodiment of formula 1(2) R5 is selected from C1-4 alkyl optionally substituted with 1 to 5 halogen atoms or a cyano group, C3-6 cycloalkyi,
isoxazolyl, pyrimidinyl and pyridinyl (and N-oxide thereof) optionally substituted with halogen.

wherein m is 0 or 1, R6a is 2-methyl-2H-tetrazol-5-yl, 3-methyI-l,2,4-oxadia2ol-5-yl, C02Ra or C(0)NHORa wherein Ra is C1-4 alkyl, particularly methyl; R6b is hydrogen, fluorine or chlorine; R3b is C1-4 alkyl, particularly methyl; R5 is selected from C1-4 alkyl optionally substituted with 1 to 5 halogen atoms or a cyano group, C3-6 cycloalkyl, isoxazoIyl, pyimidinyl and pyridinyl (and N-oxide thereof) optionally substituted with halogen or trifluoromethyl, particularly trifluoromethyl, difluoromethyl, chlorodifluromethyl, 2,2,2-trifluoroethyl, pentafluoromethyl, cyanomethyl, 5-pyrimidinyl, 5-isoxazolyl and 5-bromo-3-pyridinyl and N-oxide thereof; and R7a is hydrogen or fluorine.
Some representative compounds are:

* stereoconfiguration at the indicated carbon, § R3a is CH3
Unless otherwise stated, the following terms have the meanings indicated below:
"Alkyl" as well as other groups having the prefix "alk" such as, for example, alkoxy, alkanoyl, alkenyl, alkynyl and the like, means carbon chains which may be linear or branched or combinations thereof. Examples of alkyl groups include methyl, ethyl, propyl, isopropyl, butyl, sec- and tert-butyl, pentyl, hexyl, heptyl and the like.
"Alkenyl" means a linear- or branched carbon chain containing at least one C=C bond. Examples of alkenyl include allyl, 2-butenyl, 3-butenyl, l-methyl-2-propenyl, and the like,
"Alkynyl" means a linear or branched carbon chain containing at least one C=C bond. Examples of alkynyl include propargyl, 2-butyny!, 3-butynyl, 1-methyI-2-propynyl, and the like.
"Cyclic imide" includes succinimide, maleimide, phthalimide and the like.
"Cycloalkyl" means carbocycles containing no heteroatoms, and includes mono-, bi- and tricyclic saturated carbocycles, as well as fused ring systems. Such fused ring systems can include one ring that is partially or fully unsaturated such as a benzene ring to form fused ring systems such as benzofused carbocycles. Cycloalkyl includes such fused ring systems as spirofused ring systems. Examples of cycloalkyi include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, decahydro-naphthalene, adamantanc, indanyl, indenyl, fluorenyl, 1,2,3,4-tetrahydronaphthalene and the like,
"Haloalkyl" means an alkyl radical as defined above wherein at least one and up to all of the hydrogen atoms are replaced with a halogen. Examples of such haloalkyl radicals include chloromethyl, 1-bromoethyl, fluoromethyl, difluoromethyl, trifluoromethyl, 2;2,2-trifluoroethyl and the like.
"Halogen" means fluorine, chlorine, bromine and iodine.
"Optionally substituted" is intended to include both substituted and unsubstituted- Thus, for example, optionally substituted aryl could represent a pentafluorophenyl or a phenyl ring.

Optica! Isomers - Diastereomers - Geometric Isomers - Tautomers
Compounds described herein may contain an asymmetric center and may thus exist as enantiomers. Where the compounds according to the invention possess two or more asymmetric centers, they may additionally exist as diastereomers. The present invention includes all such possible stereoisomers as substantially pure resolved enantiomers, racemic mixtures thereof, as well as mixtures of diastereomers. The above Formula I is shown without a definitive stereochemistry at certain positions. The present invention includes all stereoisomers of Formula I and pharmaceutically acceptable salts thereof. Diastereoisomeric pairs of enantiomers may be separated by, for example, fractional crystallization from a suitable solvent, and the pair of enantiomers thus obtained may be separated into individual stereoisomers by conventional means, for example by the use of an optically active acid or base as a resolving agent or on a chiral HPLC column. Further, any enantiomer or diastereomer of a compound of the general Formula I may be obtained by stereospecific synthesis using optically pure starting materials or reagents of known configuration.
Some of the compounds described herein contain olefmic double bonds, and unless specified otherwise, are meant to include both E and Z geometric isomers.
Some of the compounds described herein may exist with different points of attachment of hydrogen, referred to as tautomers. Such an example may be a ketone and its enol form known as keto-enol tautomers. The individual tautomers as well as mixture thereof are encompassed with compounds of Formula I.
Salts
The term "pharmaceutically acceptable salts" refers to salts prepared from pharmaceutically acceptable non-toxic bases or acids. When the compound of the present invendon is acidic, its corresponding salt can be conveniently prepared from pharmaceutically acceptable non-toxic bases, including inorganic bases and organic bases. Salts derived from such inorganic bases include aluminum, ammonium, calcium, copper (ic and ous), ferric, ferrous, lithium, magnesium, manganese (ic and ous), potassium, sodium, zinc and the like salts. Preferred are the ammonium, calcium, magnesium, potassium and sodium salts. Salts prepared from pharmaceutically acceptable organic non-toxic bases-include salts of primary,

secondary, and tertiary amines derived from both naturally occurring and synthetic
sources. Pharmaceutically acceptable organic non-toxic bases from which salts can be
■J formed ir.clude, for example, arginine, betaine, caffeine, choline, N,N-dibenzyl-
ethylenediamine, diethylamine, 2-diethylaminoethanoI, 2-dimethylaminoethanol,
ethanolamine, ethylenediamine, N-ethylmorpholine, N-ethylpiperidine, glucamine,
glucosamine, histidine, hydrabamine, isopropylamine, dicyclohexylamine, lysine,
methylglucamine, morpholine, piperazine, piperidine, polyamine resins, procaine,
purines, theobromine, triethylamme, trimethylamine, tripropylamine, tromethamine
and the like.
When the compou-nd of the present invention is basic, its
corresponding salt can be conveniently prepared from pharmaceutically acceptable
non-toxic inorganic and organic acids. Such acids include, for example, acetic,
benzenesulfonic, benzoic, camphorsulfonic, citric, ethanesulfonic, fumaric, gluconic,
glutamic, hydrobromic, hydrochloric, isethionic, lactic, maleic, malic, mandelic,
methanesulfonic, mucic, nitric, pamoic, pantothenic, phosphoric, succinic, sulfuric,
tartaric, p-toluenesulfonic acid and the like. Preferred are citric, hydrobromic,
hydrochloric, maleic, phosphoric, sulfuric, and tartaric acids.
Prodrugs
The present invention includes within its scope prodrugs of the compounds of this invendon. In general, such prodrugs will be functional derivatives of the compounds of this invention which are readily convertible in vivo into the required compound. Thus, in the methods of treatment of the present invention, the term "administering" shall encompass the treatment of the various conditions described with the compound specifically disclosed or with a compound which may not be specifically disclosed, but which converts to the specified compound in vivo after admimstration to the patient Conventional procedures for the selection and preparation of suitable prodrug derivatives are described, for example, in "Design of Prodrugs," ed, H. Bundgaard, Elsevier, 1985. Metabolites of these compounds include active species produced upon introduction of compounds of this invention into the biological milieu.
Pharmaceutical Compositions
Another aspect of the present invention provides pharmaceutical compositions which comprises a compound of Formula I and a pharmaceutically

acceptable carrier. The term "composition", as in pharmaceutical composition, is intended to encompass a produc: comprising the active ingredient(s), and the inert ingredient(s) (pharmaceutically acceptable excipients) that make up the carrier, as well as any product which resul:s, directly or indirectly, from combination, complexation or aggregation of any two or more of the ingredients, or from dissociation of one or more of the ingredients, or from other types of reactions or interactions of one or more of the ingredients. Accordingly, the pharmaceutical compositions of the present invention encompass any composition made by admixing a compound of Formula I, additional active ingredient(s), and pharmaceutically acceptable excipients.
The pharmaceutical compositions of the present invention comprise a compound represented by Formula I (or pharmaceutically acceptable salts thereof) as an active ingredient, a pharmaceutically acceptable carrier and optionally other therapeutic ingredients or adjuvants. The compositions include compositions suitable for oral, rectal, topical, and parenteral (including subcutaneous, intramuscular, and intravenous) administration, although the most suitable route in any given case will depend on the particular host, and nature and severity of the conditions for which the active ingredient is being administered. The pharmaceutical compositions may be conveniently presented in unit dosage form and prepared by any of the methods well known in the art of pharmacy.
In practice, the compounds represented by Formula I, or pharmaceutically acceptable salts thereof, of this invention can be combined as the active ingredient in intimate admixture with a pharmaceutical carrier according to conventional pharmaceutical compounding techniques. The carrier may take a wide variety of forms depending on the form of preparation desired for administration, e.g., oral or parenteral (including intravenous). Thus, the pharmaceutical compositions of the present invention can be presented as discrete units suitable for oral administration such as capsules, cachets or tablets each containing a predetermined amount of the active ingredient. Further, the compositions can be presented as a powder, as granules, as a solution, as a suspension in an aqueous liquid, as a non-aqueous liquid, as an oil-in-water emulsion or as a water-in-oil liquid emulsion. In addition to the common dosage forms set out above, the compound represented by Formula I, or pharmaceutically acceptable salts thereof, may also be administered by controlled release means and/or delivery devices. The compositions may be prepared by any of

the methods of pharmacy. In general, such methods include a step of bringing into association the active ingredient with the carrrier that constitutes one or more necessary ingredients. In general, the compositions are prepared by unifonnly and intimately admiring the active ingredient with liquid carriers or finely divided solid earners or both. The product car. then be conveniently shaped into the desired presentation.
Thus, the pharmaceutical compositions of this invention may include a pharmaceutically acceptable earner and a compound or a pharmaceutically acceptable salt of Formula I. The compounds of Formula I, or pharmaceutically acceptable salts thereof, can also be included in pharmaceutical compositions in combination with one or more other therapeutically acdve compounds.
The pharmaceutical carrier employed can be, for example, a solid, liquid, or gas. Examples of solid carriers include lactose, terra alba, sucrose, talc, gelatin, agar, pectin, acacia, magnesium stearate, and stearic acid. Examples of liquid carriers are sugar syrup, peanut oil, olive oil, and water. Examples of gaseous carriers include carbon dioxide and nitrogen.
In preparing the compositions for oral dosage form, any convenient pharmaceutical media may be employed. For example, water, glycols, oils, alcohols, flavoring agents, preservatives, coloring agents and the like may be used to form oral liquid preparations such as suspensions, elixirs and solutions; while carriers such as starches, sugars, microcrystalline cellulose, diluents, granulating agents, lubricants, binders, disintegrating agents, and the like may be used to form oral solid preparations such as powders, capsules and tablets. Because of their ease of administration, tablets and capsules are the preferred oral dosage units whereby solid pharmaceutical carriers are employed. Optionally, tablets may be coated by standard aqueous or nonaqueous techniques
A tablet containing the composition of this invention may be prepared by compression or molding, optionally with one or more accessory ingredients or adjuvants. Compressed tablets may be prepared by compressing, in a suitable machine, the active ingredient in a free-flowing form such as powder or granules, optionally mixed with a binder, lubricant, inert diluent, surface active or dispersing agent. Molded tablets may be made by molding in a suitable machine, a mixture of the powdered compound moistened with an inert liquid diluent. Each tablet preferably contains from about O.lmg to about 500mg of the active ingredient and

each cachet or capsule preferably containing from about O.1mg to about 500mg of the active ingredient.
Pharmaceutical compositions of the present invention suitable for parenteral administration may be prepared as solutions or suspensions of the active compounds in water, A suitable surfactant can be included such as, for example, hydroxypropylcellulose. Dispersions can also be prepared in glycerol, liquid polyethylene glycols, and mixtures thereof in oils. Further, a preservative can be included to prevent the detrimental growth of microorganisms.
Pharmaceutical compositions of the present invention suitable for injectable use include sterile aqueous solutions or dispersions. Furthermore, the compositions can be in the form of sterile powders for the extemporaneous preparation of such sterile injectable solutions or dispersions. In all cases, the final injectable form must be sterile and must be effectively fluid for easy syringability. The pharmaceutical compositions must be stable under the conditions of manufacture and storage; thus, preferably should be preserved against the contaminating action of microorganisms such as bacteria and fungi. The carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (e.g. glycerol, propylene glycol and liquid polyethylene glycol), vegetable oils, and suitable mixtures thereof.
Pharmaceutical compositions of the present invention can be in a form suitable for topical use such as, for example, an aerosol, cream, ointment, lotion, dusting powder, or the like. Further, the compositions can be in a form suitable for use in transdermal devices. These formulations may be prepared, utilizing a compound represented by Formula I of this invention, or pharmaceutically acceptable salts thereof, via conventional processing methods. As an example, a cream or ointment is prepared by mixing hydrophilic material and water, together with* about 5 wt% to about 10 wt% of the compound, to produce a cream or ointment having a desired consistency.
Pharmaceutical compositions of this invention can be in a form suitable for rectal administration wherein the carrier is a solid. It is preferable that the mixture forms unit dose suppositories. Suitable carriers include cocoa butter and other materials commonly used in the art. The suppositories may be conveniently formed by first admixing the composition with the softened or melted carrier(s) followed by chilling and shaping in moulds.
. In addition to the aforementioned carrier ingredients, the pharmaceutical formulations described above may include, as appropriate, one or

more additional carrier ingredients such as diluents, buffers, flavoring agents, binders, surface-active agents, thickeners, lubricants, preservatives (including anti-oxidants) and the like. Furthermore, other adjuvants can be included to render the formulation isotonic with the blood of the intended recipient. Compositions containing a compound described by Formula I, or pharmaceutically acceptable salts thereof, may also be prepared in powder or liquid concentrate form.
The following are examples of representative pharmaceutical dosage forms for the compounds of Formula I:
Injectable Suspension (I.M.) mg'mL
Compound of Formula 1 10
Methylcellulose 5.0
Tween 80 0.5
Benzyl alcohol 9.0
Benzalkonium chloride 1.0
Water for injection to a total volume of 1 mL
Tablet mg tablet
Compound of Formula I 25
Microcrystalline Cellulose 415
Povidone 14.0
Pregeladnized Starch 43,5
Magnesium Stearate 2.5
500
Capsule mg/capsule
Compound of Fonnula I 25
Lactose Powder 573^
Magnesium Stearate 1,5
600
Utilities
Compounds of this invention are antagonists or inverse agonists of bradykinin receptor, in particular the bradykinin B1 receptor, and as such are useful in the treatment and prevention of diseases and conditions mediated through the

bradykinin receptor pathway such as pain and inflammation. The compounds would be effective in the treatment or prevention of pain including, for example, visceral pain (such as pancreatitis, interstitial cystitis, renal colic), neuropathic pain (such as postherpetic neuralgia, nerve injury, the "dynias", e.g., vulvodynia, phantom limb pain, root avulsions, painful traumatic mononeuropathy, painful polyneuropathy), central pain syndromes (potentially caused by virtually any lesion at any level of the nervous system), and postsurgical pain syndromes (eg, postmastectomy syndrome, postthoracotomy syndrome, stump pain)), bone and joint pain (osteoarthritis), repetitive motion pain, dental pain, cancer pain, myofascial pain (muscular injury, fibromyalgia), perioperative pain (general surgery, gynecological), chronic pain, dysmennorhea, as well as pain associated with angina, and inflammatory pain of varied origins (e.g. osteoarthritis, rheumatoid arthritis, rheumatic disease, tenosynovitis and gout).
Further, the compounds of this invention can also be used to treat hyperreactive airways and to treat inflammatory events associated with airways . disease e.g. asthma including allergic asthma (atopic or non-atopic) as well as exercise-induced bronchoconstriction, occupational asthma, viral- or bacterial exacerbation of asthma, other non-allergic asthmas and "wheezy-infant syndrome'.' Compounds of the present invention may also be used to treat chronic obstructive pulmonary disease including emphysema, adult respiratory distress syndrome, bronchitis, pneumonia, allergic rhinitis (seasonal and perennial), and vasomotor rhinitis. They may also be effective against pneumoconiosis, including aluminosis, anthracosis, asbestosis, chalicosis, ptilosis, siderosis, silicosis, tabacosis and byssinosis.
Compounds of the present invention may also be used for the Ireatment of inflammatory bowel disease including Crohn's disease and ulcerative colitis, irritable bowel syndrome, pancreatitis, nephritis, cystitis (interstitial cystitis), uveitis, inflammatory skin disorders such as psoriasis and eczema, rheumatoid arthritis and edema resulting from trauma associated with bums, sprains or fracture, cerebral edema and angioedema. They may be used to treat diabetic vasculopathy, diabetic neuropathy, diabetic retinopathy, post capillary resistance or diabetic symptoms associated with insulitis (e.g. hyperglycenmia, diuresis, proteinuria and increased nitrite and kallikrein urinary excretion). They may be used as smooth muscle relaxants for the treatment of spasm of the gastrointestinal tract or uterus. Additionally, they may be effective against liver disease, multiple sclerosis, cardiovascular disease, e.g.

atherosclerosis, congestive heart failure, myocardial infarct; neurodegenerative diseases, eg. Parkinson's and Alzheimers disease, epilepsy, septic shock e.g. as anti-hypovolemic and/or anti-hypotensive agents, headache including cluster headache, migraine including prophylactic and acute use, closed head trauma, cancer, sepsis, gingivitis, osteoporosis, benign prostatic hyperplasia and hyperactive bladder. Animal models of these diseases and conditions are generally well known in the art, and may be suitable for evaluating compounds of the present invention for their potential utilities. Finally, compounds of the present invention are also useful as research tools (in vivo and in vitro).
The compounds of this invention are useful in the treatment of pain and inflammation by the administration of a tablet, cachet, or capsule each containing, for example, O.lmg, O.5mg, Img, 3mg, 5mg, lOmg, 25mg, 50mg, lOOmg, 125mg, 250mg, or 500mg of a compound of this invention once every three to four hours, once, twice or three times a day, or (in an extended release formulation) once, twice or three times a week.
The compounds would be effective in the treatment or prevention of pain including, for example, bone and joint pain (osteoarthritis), repetitive motion pain, dental pain, cancer pain, myofascial pain (muscular injury, fibromyalgia), perioperative pain (general surgery, gynecological) and chronic pain by the administration of a tablet, cachet, or capsule each containing, for example, O.lmg, 0.5mg, Img, 3mg, 5mg. lOmg, 25mg. 50mg, lOOmg, 125mg, 250mg, or 500mg of a compound of this invention once every three to four hours, once, twice or three times a day, or (in an extended release formulation) once, twice or three times a week.
In particular, inflammatory pain such as, for example, inflammatory
airways disease (chronic obstructive pulmonary disease) would be effectively treated
«
by the compounds of this invention by the administration of a tablet, cachet, or capsule each containing, for example, O.lmg, 0.5mg, Img, 3mg, 5mg. lOmg, 25mg, 50rag, lOOmg, 125mg, 250mg, or 500mg of a compound of this invention once every three to four hours, once, twice or three times a day, or (in an extended release formulation) once, twice or three times a week-Further, the compounds of this invention can additionally be used to treat asthma, inflammatory bowel disease, rhinitis, pancreatitis, cystitis (interstitial cystitis), uveitis, inflammatory skin disorders, rheumatoid arthritis and edema resulting from trauma associated with bums, sprains or fracture by the administration of a tablet, cachet, or capsule each containing, for example, O.lmg, 0.5mg, Img, 3mg,

5mg, lOmg, 25mg, 50mg, lOOmg, 125mg, 25Qmg, or 500mg of a compound of this invention once every three to four hours, once, twice or three times a day, or (in an extended release formulation) once, twice or three times a v/eek.
They may be used subsequent to surgical interv'ention (e.g. as postoperative analgesics) and to treat inflammatory pain of varied origins (e.g. osteoarthrids, rheumatoid arthritis, rheumatic disease, teno-synovitis and gout) as well as for the treatment of pain associated with angina, menstruation or cancer by the administration of a tablet, cachet, or capsule each containing, for example, O.lmg, 0.5mg, Img, 3mg, 5mg, lOmg, 25mg, 50mg, lOOmg, 125mg, 250mg, or 500mg of a compound of this invention once every three to four hours, once, twice or three times a day, or (in an extended release formulation) once, twice or three times a week.
They may be used to treat diabetic vascuiopathy, post capillary resistance or diabetic symptoms associated with insulitis (e.g. hyperglycemia, diuresis, proteinuria and increased nitrite and kallikrein urinary excretion) by the administration of a tablet, cachet, or capsule each containing, for example, O.lmg, 0.5mg, Img, 3mg, 5mg, lOmg, 25mg, 50mg, lOOmg, 125mg, 250mg, or 500mg of a compound of this invention once every three to four hours, once, twice or three times a day, or (in an extended release formulation) once, twice or three times a week.
They may be used to treat inflammatory skin disorders such as psoriasis and eczema by the administration of a tablet, cachet, or capsule each containing, for example, O.lmg, 0.5mg, Img, 3mg, 5mg, lOmg, 25mg, 50mg, lOOmg, 125mg, 250mg, or 500mg of a compound of this invention once every three to four hours, once, twice or three times a day, or (in an extended release formuladon) once, twice or three times a week-
They may be used as smooth muscle relaxants for the treatmeric of spasm of the gastrointestinal tract or uterus or in the therapy of Crohn's disease,. ulcerative colitis or pancreatitis by the administration of a tablet, cachet, or capsule each containing, for example, O.lmg, 0.5mg, Img, 3mg, 5mg, lOmg, 25mg, 50mg, lOOmg, 125mg, 250mg, or 500mg of a compound of this invention once every three to four hours, once, twice or three times a day, or (in an extended release formulation) once, twice or three times a week.
Such compounds may be used therapeutically to treat hyperreactive airways and to treat inflammatory events associated with airways disease e.g. asthma, and to control, restrict or reverse air-vays hyperreactivity in asthma by the administration of a tablet, cachet, or capsule each containing, for example, O.lmg,

0.5mg, Img, 3mg, 5mg, lOmg, 25mg, 50mg, lOOmg, 125mg, 250mg, or 500mg of a compound of this invention onc^ every three to four hours, once, twice or three times a day, or (in an extended release formulation) once, twice or three times a week.
They may be usee to treat intrinsic and extrinsic asthma including allergic asthma (atopic or non-atopic) as well as exercise-induced broncho-constriction, occupational asthma, viral or bacterial exacerbated asthma, other non-allergic asthmas and "wheezy-infant syndrome" by the administration of a tablet, cachet, or capsule each containing, for example, O.lm.g, 0-5mg, Img, 3mg, 5mg, lOmg, 25mg, 50mg, iOOmg, 12f mg, 250mg, or 500mg of a compound of this invention once every three to four hours, once, twice or three times a day, or (in an extended release formulation) or.:e, twice or three times a week.
They may also be effective against pneumoconiosis, including aluminosis, anthracosis, asbestosis, chalicosis, pdlosis, siderosis, silicosis, tabacosis and byssinosis was well as adult respiratory distress syndrome, chronic obstructive pulmonary or airways disease, bronchitis, allergic rhinitis, and vasomotor rhinitis by the administration of a tablet, cachet, or capsule each containing, for example, 0. Img, 0.5mg, Img, 3mg, 5mg, lOmg, 25mg, 50mg, lOOmg, 125mg, 250mg, or 500mg of a compound of this invention once every three to four hours, once, twice or three times a day, or (in an extended release formulation) once, twice or three times a week.
Additionally, they may be effective against liver disease, multiple sclerosis, atherosclerosis, Alzheimer's disease, septic shock e.g. as anti-hypovolemic and/or and-hypotensive agents, cerebral edema, headache including cluster headache, migraine including prophylactic and acute use, closed head trauma, irritable bowel syndrome and nephritis by the administration of a tablet, cachet, or capsule each containing, for example, O.lmg, 0.5mg, Img, 3mg, 5mg, lOmg, 25mg, 50mg,,100mg, 125mg, 250mg, or 500mg of a compound of this invention once every three to four hours, once, twice or three times a day, or (in an extended release formulation) once, twice or three times a week.
Combination Therapy
Compounds of Formula I may be used in combination with other drugs that are used in the treatment/prevention/suppression or amelioration of the diseases or conditions for which compounds of Formula I are useful. Such other drugs may be administered, by a route and in an amount commonly used therefor, contemporaneously or sequentially with a compound of Formula I. When a

compound of Formula I is used contemporaneously with one or more other drugs, a pharmaceutical composition conraining such other drugs in addition to the compound of Formula I is preferred. Accordingly, the pharmaceutical compositions of the present invention include those tiiat also contain one or more other active ingredients, in addition to a compound of Formula I. Examples of other acdve ingredients that may be combined with a compound of Formula I, either administered separately or in the same pharmaceutical composidons, include, but are not limited to: (1) morphine and other opiate receptor agonists including propoxyphene (Darvon); (2) non-steroidal antiinflammatory drugs (NSAIDs) including COX-2 inhibitors such as propionic acid derivatives (alminoprofen, benoxaprofen, bucloxic acid, carprofen, fenbufen, fenoprofen, fluprofen, flurbiprofen, ibuprofen, indoprofen, ketoprofen, miroprofen, naproxen, oxaprozin. pirprofen, pranoprofen, suprofen, tiaprofenic acid, and tioxaprofen), acedc acid derivatives (indomethacin, acemetacin, alclofenac, clidanac, diclofenac, fenclofenac. fenclozic acid, fentiazac, furofenac, ibufenac, isoxepac, oxpinac, sulindac, dopinac, tolmetin, zidometacin, and zomepirac), fenamic acid derivadves (flufenamic acid, meclofenamic acid, mefenamic acid, niflumic acid and tolfenamic acid), biphenylcarboxylic acid derivatives (diflunisal and flufenisal), oxicams (isoxicam, piroxicam, sudoxicam and tenoxican), salicylates (acetyl salicylic acid, sulfasalazine) and the pyazoiones (apazone, bezpiperylon, feprazone, mofebutazone, oxyphenbutazone. phenylbutazone), and the coxibs (celecoxib, valecoxib, rofecoxib and etoricoxib); (3) corticosteroids such as betamethasone, budesonide, cortisone, dexamethasone, hydrocortisone, methylprednisolone, prednisolone, prednisone and triamcinolone; (4) histamine HI receptor antagonists such as bromopheniran\ine, chlorpheniramine, dexchlorpheniramine, triprolidine, clemastine, diphenhydramine, diphenylpyraline, tripelennamine, hydroxyzine,' methdilazine, promethazine, trimeprazine, azatadine, cyproheptadine, antazoline, pheniramine pyrilamine, astemizole, terfenadine, loratadine, cetirizine, desloratadine, fexofenadine and levocetirizine; (5) histamine H2 receptor antagonists such as cimetidine, famotidine and ranitidine; (6) proton pump inhibitors such as omeprazole, pantoprazole and esomeprazole; (T)leukotriene antagonists and 5-lipoxygenase inhibitors such as zafiriukast, mor.:elukast, pranlukast and zileuton; (8) drugs used for angina, myocardial ischemia including nitrates such as nitroglycerin and isosorbide nitrates, beta blockers such as ater.^lol, metoprolol, propranolol, acebutolol ,betaxolol, bisoprolol, carteolol, labetalol, nadolol, oxprenolol, penbutolol, pindolol, sotalol and dmolol, and calcium channel blockers such as dildazam, verapamil, nifedipine,

bepndil, teiodipine, tlunanzine. isradipine, nicardipine and nimodipine; ly) incontinence medications such as antimuscarinics, e.g., tolterodine and oxybutinin); (10) gastrointestinal antispasmedies (such as atropine, scopolamine, dicyclomine, antimuscarinics, as well as diphenoxylate); skeletal muscle relaxants (cyclobenzaprine, carisoprodol. chlorphenesin, chlorzoxazone, metaxalone, methocarbamol, baclofen, dantrolene, diazepam, or orphenadrine); (11) gout medications such as allopurino!. probenicid and colchicine; (12) drugs for rheumatoid arthritis such as methotrexate, auranofin, aurothioglucose and gold sodium thiomalate; (13) drugs for osteoporosis such as alendronate and raloxifene; decongestants such as pseudoep-.edrine and phenylpropanolamine; (14) local anesthetics; (15) and-herpes drugs such as acyclovir, valacyclovir and famcyclovir; and (15) anti-emetics such as ondansetron and granisetron.
Biological Evaluation
Assessing the Affinity of Selected Compounds to Bind to the Bradykinin B1 or B2
Receptor
Radioligand binding assays are performed using membranes from CHO cells that stably express the human, rabbit, rat, or dog B1 receptors or CHO cells that express the human B2 recep'or. For all receptor types, cells are harvested from culture flasks in PBS/lmM EDTA and centrifuged at 1000x2 for 10 minutes. The cell pellets are homogenized with a polytron in ice cold 20mM HEPES, InaM EDTA, pH 7.4 (lysis buffer) and centrifuged at 20,000xg for 20 minutes. The membrane pellets are rehomogenized in lysis buffer, centrifuged again at 20,000xg and the final pellets are resuspended at 5mg protein/ml in assay buffer (120mM NaCl, 5mM KCl, 20mM HEPES, pH 7.4) supplemented with 1% BSA and frozen at-80°C.
On the day of assay, membranes are centrifuged at 14,000xg for 5 minutes and resuspended to the desired protein concentration in assay buffer containing lOOnM enaliprilat, 140µg/mL bacitracin and 0,1% BSA. 3H-des-arglO, leu9 kallidin is the radioligand used for the human and rabbit B1 receptors, 3H-des-arglO kallidin is used for the rat and dog Bl receptors, and 3H-bradykinin is used to label the human B2 receptor.
For all assays, compounds are diluted from DMSO stock solutions with 4µL added to assay tubes for a final DMSO concentration of 2%. This is followed by the addition of lOOµL radioligand and lOOµL of the membrane suspension. Nonspecific binding for the Bl receptor binding assays is determined

using lµM des-arglO kallidjn and nonspecific binding for the B2 receptor is determined with lµM bradykin. Tubes are incubated at room temperature (220C) for 60 minutes followed by filtranon using a Tomtec 96-well harvesting systeni. Radioactivity retained by the filler is counted using a Wallac Beta-plate scintillation counter.
The compounds of this invention have affinity for the Bl receptor in the above assay as demonstrated by results of less than SpcNL It is advantageous that the assay results be less than lµM, even more advantageous for the results be less than 0.5MM. It is further advanrageous that compounds of this invention have affinity for the bradyidnin Bl receptor over the bradykinin B2 receptor; more advantageously, the affinity for the Bl receptor is at least 10 fold, and preferably over 100 fold, over that for the B2 receptor.
Assay for Bradykinin Bl Antagonists
Bl agonist-induced calcium mobilization was monitored using a Fluorescence Imaging Plate Reader (FLIPR). CHO cells expressing the Bl receptor were plated in 96 or 384 well plates and allowed to incubate in Iscove's modified DMEM overnight. Wells were washed two times with a physiological buffered salt solution and'then incubated with 4uM Fluo-3 for one hour at 370C. The plates were then washed two times with buffered salt solution and lOOuL of buffer was added to each well. Plates were placed in the FLIPR unit and allowed to equilibrate for two minutes. The test compound was then added in 50ul volumes followed five minutes later by 50ul of agonist (des-arglO kallidin). Relative fluorescence peak heights in the absence and presence of antagonist were used to calculate the degree of inhibition of the Bl receptor agonist response by the test compound. Eight to ten concentrations of test compound were typically evaluated to construct an inhibition curve and detennine IC50 values using a four-parameter nonlinear regression curve fitting routine.
Assay for Bradykinin Inverse Agonists
Inverse agonist acrivity at the hunaan Bl receptor was evaluated using transiently transfected HEK293 cells. One day following transfection cell flasks were labeled overnight with 6uCi/ml pHjmyo-inositoI. On the day of assay, the media was removed and the attached cells were gently rinsed with 2x20ml of phosphate-buffered saline. Assay buffer (HEPES buffered physiological salts, pH 7.4) was added and the cells were detached by tapping of the flask. The cells were centrifuged at 800xg for

five minutes and resuspended a: lxl06 cells/ml in assay buffer supplemented with lOmM lithium chloride. After 10 minutes at room temperature, one-half ml aliquots were distributed to tubes containing test compound or vehicle. After an additional 10 minutes the tubes were transferred to a 37°C water bath for 30 minutes. The incubation was terminated by the addition of a 12% perchloric acid solution and the tubes were placed on ice for 30 .Tiinutes. The acid was then neutralized with KOH and the tubes centrifuged to pellet precipitated material [3H]Inosito[ monophosphate formed was recovered by standard ion exchange chromatographic techniques and quantitated by liquid scintillation counting. Inverse agonist activity was determined by the degree to which a test compound reduced basal (cells incubated with vehicle) levels of [3H]inositol monophosphate accumulation.
Abbreviations Used
The following abbreviations have the meanings indicated, unless stated otherwise in the specification:
BOC (boc) t-butyloxycarbonyl
DCM dichloromethane
DMF dimethylformamide
DMSO Dimethyl sulfoxide
EDC or EDCI l-(3-dim.ethyIaminopropyl)3-ethylcarbodiimide HCl
eq. equivalen.t(s)
ES (or ESI) - MS electron spray ionization - mass spectroscopy
Et ethyl
EtOAc ethyl acetate
EtOH ethane!
FAB-MS fast atom bombardment-mass spectroscopy
HOBt 1-hydroxybenzotriazole hydrate
HPLC high pressure liquid chromatography
LCMS Liquid chromatography/mass spectroscopy
LHMDS lithium bis(triraethylsilyl)amide
Me methyl
MeOH Methanol
MHz megahertz
MsCl Mesyl chloride

NEt3 Triethylamine
NMR nuclear magnetic resonance
TFA trifluoroacetic acid
THF tetrahydrofuran
Compounds of formula I may be prepared according to the following illustrative schemes.

In Scheme 1, compound (la) is assembled by coupling the biaryhnethanamine derivative (1) to the protected aminocycloalkanoic acid (2) using standard peptide coupling reagent combinations, such as EDCI/HOBt, in an appropriate solvent, such as THF, to provide (3). The Boc protecting group is then removed by the action of an acid, like HCl, in an appropriate solvent, like MeOH, to yield an ammonium salt from which the free-base derivative (4) may be obtained using an appropriate base, such as ammonia, and an appropriate solvent, such as

chloroform. This amine deriva:ive (4) is then reacted with a carboxylic acid or carboxylic acid equivalent to yield title compound (la). Alternatively, the acid-salt of (4) can be used in the final reaction to yield title compound (la) provided an appropriate base such as triethylamine is added.
Alternatively, compound (la) may be assembled by coupling the biarylmethanamine derivati\e (2), with the acylated aminocycloalkanoic acid (5) as shown in Scheme la.

In Scheme 2a, the cyanobiaryl derivative (S) is assembled using a Suzuki reaction between an aromatic boronic acid derivative (6), or an appropriate boronic ester derivative, and an aromatic halide (7) in the presence of a triarylphosphine, like triphenylphosphine, and a metal catalyst, like palladiuna acetate. The resultant cyano biaryl intermediate (8) is then catalytically reduced to the corresponding amine biaryl derivative (la) using hydrogen and a metal, such as Raney Ni, in an appropriate solvent.
Alternatively, as illustrated in Scheme 2b, a methanamine derivative (9), after primary amine protection with an appropriate protecting group such as Boo, is elaborated to the pinacol boron ester (11) using a palladium catalyst in an appropriate solvent, like dimethyl sulfoxide. This boron ester (11) is coupled^to an aryl halide derivative (7) employing Suzuki reaction conditions to yield (1).

A third method for the preparation of biarylmethanamine derivatives is depicted in Scheme 2c. The biaryl moiety (14) is first assembled using a palladium catalyzed coupling of (12) with an aryl zinc compound (13) as shown. The methyl group of biaryl (14) is then elaborated according to the three step sequence of halogenation, nucleophilic displacement of the halogen with azide, and reduction to provide the corresponding amine intermediate (la). Alternatively, the biarylmethanamine (la) can also be prepared starting from the arylcarbonitrile (16) and aryl zinc compuond (13) as previously discussed. The resulting biarylcarbonitrile (8) is then reduced using hydrogen to provide (la).

It will be appreciated by persons skilled in the art that functional group interconversion can be used to provide various compounds of formula I. As illustrated in Scheme 3, derivative (3a) is bis-deprotected first by the action of a strong acid, like TFA, and second by alkaline hydrolysis in a suitable mixture of water and an organic solvent, like methanol, at a temperature between 25 and 100 °C to yield the amino acid derivative (17). Prior activation of a carboxylic acid (R5COOH) with an appropriate set of peptide coupling reagents, like EDCI/HOBt, forms the 'active ester' which then reacts with the amino acid derivative (17) to yield (18). The latter compound can either react with amines (HNRbRc) or alkyloxy amines (H2N0Ra) under the action of an appropriate set of peptide coupling reagents, like
EDCI/HOBt, to form the claimed compounds (lb) and (Ic), respectively.

N-alkylation is illustrated in Scheme 4. The amine (4) is alkylated with excess alkyl iodide (I-Rl) in an appropriate solvent, like THF, in the presence of

The preparation of compounds of fonnula I having a 1,2-cis- or 1,2-trans-cyclopropyl moiety is illustrated in Schemes 5 and 6, According to known procedures (K. Burgess et al., J. Org. Chem., 57:5931-5936(1992)), di-ttert-butyl malonate is elaborated to derivative (20). The N-Boc group is removed using methane sulfonic acid according to L. S. Lin et al. Tetrahedron Lett, 41:7013-7016(2000) to give amine (21). This amine is allowed to react with a carboxylic acid or carboxylic acid equivalent under appropriate peptide coupling conditions to yield (22). The tert-, butyl ester is then cleaved with an acid, like TFA, in an appropriate solvent, like DCM, to provide acid (23). Biarylmethanamine (1) is then coupled with the acid (23)

using an appropriate set of peptide coupling reagents, like EDCI/HOBt, to produce the title compound (le). Further elaboration of (le) to additional compounds of formula I may be accomplished using procedures well known to those skilled in the art. For example, the acetyl group may be removed by hydrolysis to provide the con'esponding alcohol; the alcohol may be convened to the corresponding sulfonate by treatment with sulfonyl chloride, and the sulfonate may be converted to the corresponding halide by treatment with a source of the halide. These and other functional transformations to provide compounds of formula I are described in typical organic chemistry textbooks such as March's Advanced Organic Chemistry: Reactions, Mechanisms, and Structure. 5th Ed., John Wiley & Sons, 2000.

In Scheme 6, according to known procedures (K. Burgess et al.,7. Org.
Chem., 57:5931-5936(1992)), di-tert-butyl malonate is elaborated to derivative (24).
The N-Boc group is removed using an acid, like TFA, in an appropriate solvent, like
DCM. This amine is allowed to react with a carboxylic acid or carboxylic acid equivalent under appropriate peptide coupling conditions, like EDCI/H0Bt/NEt3 to
yield (25). Biarylmethanamine (1), is then allowed to open the lactone (25) in an appropriate aprotic solvent, like D!vIF, at a temperature between 20 and 100 °C, to produce the title compound (If). Further elaboration of (If) to additional title compounds may be accomplished using procedures well known to those skilled in the art as previously discussed.

The following examples are provided to illustrate the invention without limiting the invention to the particulars of these examples. Compounds were named using: ACD/Name version 4.53 (Advanced Chemistry Development Inc. © 1994-2000). Address: 90 Adelaide Street West, Toronto, Ontario, M5H 3V9, Canada.
EXAMPLE 1 Methyl 3-fluoro-4*.{(lR)-l-[({ l-[(3,3,3-trifluoropropanoyl)ajnino]cyclopropyl}-carbonyI)amino]ethyl} -1,1 '-biphenyl-2-carboxylate

Commercially available (lR)-l-(4-bromophenyl)ethanamine was Boc protected, using standard procedures known to those skilled in the art, to produce tert-butyl (lR)-l-(4-bromophenyl)e:hylcarbamate.
To a solution of tert-butyl (lR)-l-(4-bromophenyl)ethylcarbamate (7.6
g, 25.3 mmol) in DMSO (20 mL) was added bis(pinacolato)diboron (7.07 g, 27.9
mmol), dichloro[l,1-bis(diphenylphosphino)ferrQcene]palIadium (II) dichloro-
methane adduct (2.06 g, 2.53mmol), and potassium acetate (7.45 g, 76.0 mmol) at room temperature under N2. The resulting mixture was heated at 80 °C for 1 hour.
The reaction was quenched by addition of EtOAc and filtered through celite. The
organic extract was washed with water three times, saturated NaCl, dried over MgS04, filtered and concentrated under vacuum. The residue was purified on silica
gel eluted with 0-20% ethyl acetate in hexane to provide tert-butyl (lR)-l-[4-(4,4,5,5-tetramethyI-l,3,2-dioxaborolan-2-yl)phenyl]ethyIcarbamate as a clear light yellow oil with a mass ion (ES+) of 333.
To a stirred solution of tert-bntyl (lR)-l-[4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)phenyI]ethyIcarbamate (1.0 g, 2.9 mmol) and methyl 2-fluoro-6-iodobenzoate (1.2 g, 4.32 mmol) in 25 mL of a 5:1 THF:water mixture was added potassium carbonate (1.2 g, 8.64 mmol), tri-0-tolyIphosphine (350 mg, 1.15 mmol) and lastly palladium acetate (65 mg, 0.29 mmol). The reaction vessel was then sealed and placed into a 90 T oil bath for overnight stirring and heating. After about 18 hours the reaction mixture was cooled to ambient temperature and then diluted with EtOAc. The organics were washed with brine (x4), dried over sodium sulfate, filtered, and concentrated under reduced pressure to give an oil. This oil was subject to silica gel chromatography eluting with 10-60% EtOAc in hexanes to provide methyl 4'-{(lR)-I-[(tert-butoxycarbonyl)amino]ethyI}-3-fluoro-l,1-biphenyl-2-carboxylate (205 mg), found to be pure by LC/MS and proton NMR.

Methyl 4-{(IR)-l-[(r(?rr-butoxycarbonyl)amino]ethy!}-3-fluoro-l,r-biphenyl-2-carboxylate (205 mg, 0.60 mmol) dissolved in MeOH (15 mL) was cooled to 0 °C. This homogenous solution was saturated with anhydrous hydrogen chloride and allowed to sit for 20 minutes. Dry nitrogen was then bubbled through the solution for about 30 minutes. Solvent was then removed under reduced pressure to yield an oilv residue. The oil was then dissolved in DCM and the solvent removed. This process was repeated until a solid amine hydrochloride was obtained.
The above amine hydrochloride (85 mg, 0.27 mmol) along with 1-
[(tert-butoxycarbonyl)amino]cyclopropanecarboxylic acid (55 mg, 0.27 mmol), H0BfH20 (8.4 mg, 0.05 mmol) and triethylamine (33 mg, 0.33 mmol) were
dissolved in 4.5 mL of THF. To this room-temperature solution was added EDCI (74
mg, 0.38 mmol). After overnight stirring (ca. 16.5 h) the reacdon mixture was diluted
with water and EtOAc. The organic layer was washed successively with IN HCl, 5%
sodium bicarbonate, half-brine (x3) and then brine. The organic layer was dried over
sodium sulfate, filtered and evaporated under reduced pressure to obtain a residue
which was subjected to silica gel chromatography elating with 1-6% MeOH in DCM.
Collection of product containing fractions and removal of solvent yielded 108 mg
(86%) of methyl 4'-((lR)-l-[(( l-[(tert-butoxycarbonyl)amino]cyclopropyl)carbonyl)-
amino]ethyl)-3-fluoro-l,r-biphenyl-2-carboxylate.
Methyl 4'-{(lR}-l-[({ I-[(tert-butoxycarbonyI)amino]cyciopropyl}-
carbonyl)amino]ethyl}-3-fluoro-l,r-biphenyl-2-carboxylate (108 mg, 0.24 mmol)
dissolved in MeOH (5.0 mL) was cooled to 0 °C. This homogenous solution was
saturated with anhydrous hydrogen chloride and allowed to sit for 30 minutes. Dry
nitrogen was then bubbled through the solution for about 50 roin. Solvent was then
removed under reduced pressure to yield an oily residue. The oil was then dissolved
in DCM and the solvent removed. This process being repeated until a solid amine
hydrochloride was obtained-
The above amine hydrochloride (46 mg, 0.12 mmol) along with trifluoropropionic acid (15 mg, 0.12 mmol), H0Bt«H20 (3.6 mg, 0.02 mmol) and
triethylamine (14 mg, 0.14 mmol) were dissolved in 1.6 mL of THF plus 1.6 mL of DIvIF. To this room-temperature solution was added EDCI (31 mg, 0.16 mmol). After overnight stirring (ca, 18 h) the reaction mixture was diluted with water and EtOAc. The organic layer was washed successively with IN HCl, 5% sodium bicarbonate, half-brine (x3) and then brine. The organic layer was dried over sodium sulfate, filtered and evaporated under reduced pressure to obtain a residue which was

subjected to silica ge! chromatography eluting with 1-12% MeOH in DCM.
Collection of product containing fractions and removal of solvent yielded 36 mg
(67%) of the title compound as a foaming solid. Purity was determined by LCMS (ES MS, M+H+ found:467) and proton NMR (400 MHz, CD3OD : 5 7.555, 7.540, 7.535,
7.520, 7.515, 7.500, 7.393. 7.373, 7.319, 7.302, 7.298, 7.240, 7.222, 7.221, 7.211, 7. ISS, 7.167, 7.165, 5.116, 5.099. 5.081, 5.064, 3.659, 3.268, 3.241, 3.214, 3.1S7, 1.508, 1.490, 1.483, 1.477, 1.474, 1.470, 1.465, 1.454, 1.444, 1.056, 1.049, 1.036, 1.031, 1.023, 1.007,0.999,0.995,0.982,0.974).

A solution of methyl 4-{(lR)-l-[({l-[(tert-butoxycarbonyl)amino]-
cyclopropyl}carbonyl)amino]ethyl}-3-fluoro-1,1-biphenyl-2-carboxylate (466 mg, 1.0 mmol) in DCM (15 inL) and TFA (15 mL) was stirred under N2 for 20 minutes at
ambient temperature, then the organic solvent was removed under vacuum. The
residue was dissolved in MeOH (20 mL), 4N NaOH (10 mL) and water (10 mL).
This mixture was heated at reflux for 4 hours and then neutralized with 6N HCl. Purification was achieved by preparative HPLC on a delta-pack Cig column, 300 A,
pore size 15 µM with 0.05% HCI acid -aqueous acetonitrile solvent systems using various linear gradients. Fracdons containing product of 99% purity as measured by HPLC were combined and lyophilized to give 4*-((lR)-l-{[(l-aminocyclopropyl)-carbonyl]amino}ethyl)-3-fluoro-1,1-biphenyl-2-carboxy]ic acid as a white solid.
To a solution of trifluoropropionic acid (128 mg, 1.0 mmol) in DCM (1 mL), l-ethyl-(3-dimethylaniincpropyl)carbodiimide hydrochloride (229 mg, 1.2

mmol) and l-hydroxy-7-azabenzotriazole (136 mg, 1.0 mmol) were added. The
resulting solution was stirred et room temperature for 20 minutes, then 4'-((lR)-l-
{[(l-aminocyclopropyl)carbonyl]amino}ethyl)-3-fluoro-l,r-biphenyl-2-carboxylic
acid (171 mg, 0.5 mmol) in mL DCM was added, followed by MA^-diisopropylethyl-
amine until pH = 10 was achieved. The reaction mixture was stirred at ambient temperature under N2 for 2 hours, concentrated under vacuum and then partitioned
between water and ethyl acetate. The organic extract was washed with brine, dried
over anhydrous magnesium sulfate, filtered and concentrated under vacuum. The
residue was subjected to co!urr.n chromatography on silica gel eluted with 40% MeOH in CHCI3. Collection and concentration of appropriate fractions provided 3-
fluoro-4-{(lR)-l-[({l-[(3,33-:rifluoropropanoyl)amino]cyclopropyl}carbonyl)-
amino]ethyl}-l,l-biphenyl-2-carboxylic acid as a white powder.
To a solution of the above acid (226 mg, 0.50 mmol) in DCM (i mL),
l-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride (134 mg, 0.70 mmol), 1-
hydroxy-7-azabenzotriazole (6Smg, 0.50 mmol) and methoxyamine hydrochloride
(167 mg, 1.0 mmol) were added, followed by N N-diisopropylethylamine until pH =
10 was achieved. The resulting solution was stirred at room temperature for 2 hours,
and then partitioned between e:hyl acetate and water. The organic extract was washed
with brine, dried over anhydrous magnesium sulfate, filtered and concentrated under vacuum. Purification was achieved by preparative HPLC on a delta-pack C18 column
with 0.05% HCl acid -aqueous acetonitrile solvent systems using various linear gradients. Fractions containing product of 99% purity as measured by HPLC were combined and lyophilized to give the title compound as a white solid.. Purity was determined by LCMS (ES MS, M+H+ found:482) and proton NMR (400 MHz, DMS0-d6) 5 1.40 (d, J = 7.1 Hz, 3H), 0.60-0.80 (m, 2H), L27 (m, 2H), 3.23 (m, J =
11.2 Hz, 2H). 3.44 (s, 3H), 5.02 (q, 7= 8 Hz, 1H), 7.25-7.39 (m, 6H), 7.52 (m, 1H), 7.93 (d, j= 8.2Hz, 1H), 8.89 (s, 1H).
EXAMPLE 3
Methyl 3-fluoro-4-{ l-methyl-l-[({ l-[(trifluoroacetyl)amino]cycIopropyl}-carbonyl)amino]ethyI} -1,1 -biphenyl-2-carboxylate

Methyl 2-(4-bromopheny!)-2-methylpropanoate (3.75 g, 14.6 mmol, prepared according to J. Org. Chem., 59:2620-2622(1994)) in 150 mL of THF was allowed to react with potassium trimethylsilanolate (2.62 g, 20.4 mmol) at ambient temperature, for 60 hours, with continuous stirring. The reaction was then diluted with water and DCM. The pH of the aqueous phase was then adjusted to ca. 4, using IM HCl. The aqueous layer was then extracted three times with additional DCM. The organic layers were pooled, dried over sodium sulfate, filtered and then concentrated to obtain 3.59 g cf 2-(4-bromophenyl)-2-methylpropanoic acid, as a white solid, which gave LC/MS and proton NMR spectra consistent with theory.
To 82 mL of toluene was added the above acid (2.00 g, 8.23 mmol), triethylamine (1.20 mL, 8.64 mmol) and diphenylphosphoryl azide (1.86 mL, 8.64 mmol). After refluxing this mixture under nitrogen for 1 hour, benzyl alcohol (1.70 mL, 16.5 mmol) was added and the reaction mixture was allowed to reflux overnight. Solvent was removed under reduced pressure and the resulting oil was diluted with ethyl acetate. The ethyl acetate solution was washed twice with 5% sodium bicarbonate and once with brine. The organic layer was dried over sodium sulfate, filtered and evaporated under reduced pressure to obtain a residue which wassubject to silica gel chromatography eluting with 0-2% MeOH in DCM. Collection of product containing fracdons and removal of solvent yielded 2.26 grams of benzyl N-[l-(4-bromophenyl)-l-methylethyl]carbamate, which gave LC/MS and proton NMR spectra consistent with theory.
The above bromide (LOO g, 2.87 mmol) was added to 25 mL of DMF, followed by bis(pinacolato)boron (0.875 g, 3.45 mmol), potassium acetate (0.846 g, 8.62 mmol) and PdCl2(dppf)2*CH2Cl2 (.063 g, 0.090 mmol). This mixture was
heated to 80 °C, under nitrogen, for 3 hours. After cooling to ambient temperature, methyl 2-fluoro-6-iodobenzoate (0.965 g, 3.45 mmol), PdCl2(dppf)2'CH2Cl2 (.063 g,

0.090 mmol) and aqueous sodium carbonate (7.18 noL, 2M, 14.4 mmol) were added. This mixture was then heated to 80 °C overnight. After coolins to ambient temperature, most of the DMF was removed under reduced pressure and the biphasic mixture was diluted with ethyl acetate. The pH of the aqueous layer was made neutral with IM HCl, prior to extraction with two additional volumes of ethyl acetate. The combined organic layers were dried over sodium sulfate, filtered and evaporated under reduced pressure to obtain a residue which was subject to silica gel chromatography eluting with 0-2% MeOH in DCM. Collection of product containing fractions and removal of solvent yielded 0.25 grams of methyl 4'-(l-{[(benzyloxy)carbonyl]amino}-l-methylethyl)-3-fIuoro-l,r-biphenyI-2-carboxylate, which gave LC/MS and proton NMR spectra consistent with theory.
Methyl 4'-(l-{ [(benzyloxy)carbonyl]amino}-l-methylethyl)-3-fluoro-l,r-biphenyl-2-carboxylate (0.25 g, 0.59 mmol) was dissolved in 6.0 mLof anhydrous ethanol. Pd/C (90 mg) was then added and the nitrogen atmosphere was exchanged for hydrogen. The reacdon mixture was allowed to stir for 72 hours. After filtration chough celite, the ethanol was removed under reduced pressure to yield 0.15 grams of methyl 4'-(l-amino-l-methylethyl)-3-fluoro-l,r-bipheny!-2-carboxylate which was of sufficient purity to use directly in the next reaction.
The above-mentioned amine (80 mg, 0.28 mmol), was dissolved in
anhydrous DCM (4 mL). To this stirred solution was added l-[(tert-butoxycarbonyl)-aminojcyclopropanecarboxylic acid (67 mg, 0.33 mmol), H0Bf-H20 (8 mg, 0.08
mmol) and lastly EDCI (69 g, 0.36 mmol). This mixture was allowed to stir overnight. Solvent was then removed under reduced pressure and the residue was subjected to silica gel chromatography eluting with a 1-3% MeOH in DCM gradient to provide methyl 4-{!-[({l-[(tert-butoxycarbonyl)amino]cyclopropyl}carbbnyl)-, amino]-l-methylethyl}-3-fluoro-l,r-biphenyl-2-carboxylate(82 mg), giving LC/MS and proton NMR spectra consistent with theory.
The above material (82 mg, 0.17 mmol) dissolved in ethyl acetate (4 mL) was cooled to 0 °C. This homogenous solution was saturated with anhydrous hydrogen chloride and allowed to sit for 30 minutes- Dry nitrogen was then bubbled through the solution for about 30 minutes. Solvent was then removed under reduced pressure to yield an oily residue, which was used directly in the next reaction.
To a 0 °C, stirred solution of the above mentioned amine (32 mg, 0.09 mmol) in DCM (2.0 mL) was added triethylamine (20 µL, 0.13 mmol) and lastly trifluoroacetic anhydride (20 µL, 0.11 mmol). After 10 minutes the ice bath was

remove and stirring was continued for a total of 30 minutes The reaction mixture was subject to silica gel chromatography elating with 0.5-2% MeOH in DCM. Collection of product containing fractions and removal of solvent yielded 34 mg (84%) of the title compound as a foaming solid. Purity was determined by LCMS (ES MS, M + H+ found:467) and proton KMR (400 MHz, CD3OD : 5 7.552, 7.537. 7.531, 7.517,
7.512, 7.497. 7.442, 7.425, 7.420, 7.302, 7.297, 7.280, 7.250, 7.231, 7.203, 7.181, 7.159,3.644, 1.664, 1.444, 1.432, 1.424, 1.412, 1.080, 1.068, 1.060. 1.048).

To a solution of 2-fluoro-6-iodobenzoic acid (iS.OOg, 56.39 mmol) in 150 mLCH2Cl2 containing 0.1 mLDMF was added oxalyl chloride (9.30g, 73.3
mmol) dropwise. The solution was stirred at room temperature for 75 minutes, then concentrated in vacuo. The residue was re-dissolved in 150 mL CH2CI2, and the
solution was saturated three times with ammonia gas. The solution was concentrated in vacuo and dried under vacuum overnight. The residue was dissolved in NJN-dimethylacetamide dimethyl acetal (24.7 mL, 0.169 mol) and heated to 120 ""C for 5 hours. Additional N,N-dimethylacetamide dimethyl acetal (25 mL, 0.17 mol) was added over the course of the reaction to drive it to completion. The solution was cooled to room temperature, concentrated in vacuo, and dried under vacuum overnight. To a solution of the intermediate in 57 mL dioxane was added hydroxylamine hydrochloride (4.704g, 67.69 mmol), 5N NaOH (13.5 mL, 67.7 mmol), and 70% acetic acid (57 mL). The mixture was stirred at 60 °C for 2 hours, then at 90 °C for 3 hours. The resulting solution was cooled to room temperature,

diluted with ethyl acetate, and neutralized with aqueous sodium bicarbonate. The organic extract was washed with aqueous sodium bicarbonate and brine, dried over sodium sulfate, filtered and concentrated under vacuum. The residue was filtered through silica gel eluted with 10% ethyl acetate in hexanes to provide 5-(2-fluoro-6-iodophenyl)-3-methyl-L2.4-c\adiazo!e as orange yellow crystals that gave proton NlvIR spectra consistent with theory and a mass ion (ES+) of 305.06 for M+H+.
To a solution o: (S)-(-)-2-methyl-2-propanesuIfinamide (20.20 g, 0.167 mol) in 350 mL CH2CI2 were added 4-bromo-2-fluorobenzaldehyde (35.53g, 0.1750
mol), pyridinium-p-toIuene5u:fonate (2.09g, 8.33 mmol), and magnesium sulfate (200.6 g, 1.667 mol). The reaction mixture was stirred at room temperature for 48 hours. Additional magnesium sulfate (100.3g , 0.833 mol) was added, and the reaction was stirred 24 hours. The mixture was filtered through celite, washing with CH2CI2 and concentrated in vacuo. The resulting residue was subjected to column
chromatography on silica gel eluted with 0-10% ethyl acetate in hexanes to afford N-[(lE)-(4-bromo-2-fluorophenyl)methylidene]-2-methylpropane-2-sulfinamide as a white solid that gave proton NMR spectra consistent with theory and a mass ion (ES+) of 308.09 forM+H+(SlBr).
To a solution of N-[(iE)-(4-bromo-2-fluorophenyl)methylidene]-2-methylpropane-2-sulfinamide (32.65g, 0.1066 mol) in 550 mL CH2CI2 at -48 °C was
added methylmagnesium chioride (3.0 M solution is ether, 53.31 mL, 0.1599 mol)
dropwise. The reaction was quenched with aqueous ammonium chloride and the
aqueous layer was extracted with methylene chloride. The combined organics were dried over Na2S04, filtered and concentrated under vacuum. The resulting residue
was subjected to column chromatography on silica gel eluted with 10-50% ethyl acetate in hexanes to afford N-[(lR)-l-(4-bromo-2-fluorophenyl)ethyl]-2-methyl-propane-2-sulfinainide as a white solid that gave proton NMR spectra consistent with theory and a mass ion (ES+) of 324,14 for M+H+(8lBr).
To a solution of N-[(lR)-l-(4-bromo-2-fluorophenyl)ethyl]-2-methyl-propane-2-sulfinamide (26.29g, 81.58 mmol) in 40 mL methanol was added HCl/ dioxane (4M, 40,8 mL, 0.163 mol) solution. The reaction mixture was concentrated in vacuo, and ether was added. The white solid was collected, washing with cold ether, and dried under vacuum to yield (lR)-l-(4-bromo-2-fluorophenyl)ethan-aminium chloride that gave proton NMR spectra consistent with theory.
To a solution of the above (lR)-l-(4-bromo-2-fluorophenyl)-ethan-aminium chloride (14.24g, 55.95 mmol) in 300 mL CH2CI2 at 0 °C was added di-ten-

butyl dicarbonate (17.9Sg, 82.40 mmol) and triethylamine (8.256 g, 81.58 mmol). The solution was washed with water and brine, dried over Na2S04, filtered and
concentrated under vacuum to provide crude (lR)-l-(4-bromo-2-fluorophenyl)ethan' aminium chloride as a \\hite solid that gave proton NMR spectra consistent with theory.
A mixture of i lR)-l-(4-bromo-2-f]uorophenyr]ethanamimum chloride (26.42g, 83.03 mmol), bi5(pinacolato)diboron (31.63g, 0.1246 mol), potassium acetate (24.45 g, 0.2491 mol), and [l,1r-bis(diphenylphosphino)ferrocene]-
palladium(D) dichloride (0.265 g, 0.362 mmol) in SO mL DMSO was heated to 90 °C under N2 for 3 hours. The mixture was then cooled to room temperature and
partitioned between ethyl acetate and water. The organic extract was washed with water and brine, dried over Na2S04, filtered and concentrated under vacuum. The
residue was subjected to silica gel chromatography eluted with 0-10% ethyl acetate in
hexanes to provide tert-butyl (lR)-l-[2-fluoro-4-(4,4,5,5-tetramethyl-l,3,2-dioxa-
borolan-2-yl)phenyl]ethylcarbonate as a beige solid that gave proton NMR spectra
- consistent with theory.
A mixture of 5-(2-fluoro-6-iodophenyI)-3'methyl-l,2,4-oxadiazole
(1.50g, 4.93 mmol), tert-butyl (lR)-l-[2-fluoro-4-(4,4,5,5-tetramethyl-l,3,2-dioxa-
borolan-2-yl)phenyl]ethylcarbonate (1.80 g, 4.93 mmol), potassium carbonate (1.70 g,
12.3 mmol), tri-o-tolyiphosphine (0.060 g, 0.20 mmol), and palladium acetate (0.011
g, 0.05 mmol) in 25 mL of THF and 4 mL of water was heated in a sealed flask at
100°C overnight. The mixture was then cooled and concentrated in vacuo. The
resulting residue was dissolved in ethyl acetate, washed with water and brine, dried over Na2S04, filtered and concentrated under vacuum. The residue was subjected to
silica gel chromatography eluted with 0-10% ethyl acetate and hexane to provide tert-butyl (lR)-l-[33'-ciifluoro-2'-(3-methyl-l,2,4-oxadia2ol-5-yl)-l,r-biphenyM-yl]-ethylcarbamate as a yellow oil that gave proton NMR spectra consistent with theory. The product was dissolved in ethyl acetate and saturated with HCI gas. The solution was concentrated in vacuo and azeotroped 3 times with toluene to provide (1R)-1-
[3,3-difluoro-2'-(3-methyI-l,2,4-oxadia2ol-5-yI)-l,r-biphenyl-4-yI]ethanamihium chloride.
A solution of (lR)-l-[3,3'-difluoro-2'-(3-methyl-1,2,4-oxadia2ol-5-yl)-l,r-biphenyl-4-yl]ethanaminiuni chloride (O.SOOg, 1.42 mmol), Boc-l-aminocyclo-propane-l-carboxylic acid (0.3C0g, 1.49 mmol), l-ethyl-(3-dimethylaminopropyl)-carbodiimide hydrochloride (0.545 g, 2.84 mmol), l-hydroxy-7-azabenzotriazole

(0.010 g, 0.15 mmol), and tnethylamine (0.863 g, 8.53 mmoi) in 10 mL CH2CI2 was
stirred at room temperature overnight. The solution was washed with aqueous sodium bicarbonate and brine, dnec over Na2S04, filtered and concentrated. The residue was
subjected to silica gel chrorr. Jtography eluted with 10-40% ethyl acetate in hexanes to provide tert-butyl 1-[({(1R;)-1-[3,3-difluoro-2H3-methyl-l,2.4-oxadiazol-5-yl)-l,r-bipheny]-4-yI]ethyl}amino>carbo^yl]cyclop^opylcarbamate as a white solid that gave proton NMR spectra consistent with theory. The product was dissolved in ethyl acetate and saturated with KCl sas. The solution was concentrated in vacuo and azeotroped 3 times with toluene to provide l-[(((lR)-l-[3,3-difIuoro-2-(3-methyl-l,2,4-o\adiazol-5-yl)-l,l,'biphenyl-4-yl]ethyl}amino)carbonyl]-cyclopropanaminium chloride that gave a mass ion (ES+) of 399.21 for M+H+.
To a solution of the above compound (0,290 g, 0.667 mmol) in 5 mL CH2CI2 at 0 °C was added trlethylamine (0.135 g, 1.33 mmol) and trifluoroacetic
anhydride (0,14 g, 0.67 mmoi). The solution was diluted with addition CH2CI2 and
washed with aqueous sodium bicarbonate and brine, dried overNa2S04, filtered and
concentrated. The residue was subjected to silica gel chromatography eluted with 10-
40% ethyl acetate in hexanes :o provide the title compound that gave proton NMR
spectra consistent with theory and a mass ion (ES+) of 495.22 for M+H+: 1H NMR (300 MHz, MeOH-d4) 5 9.71 (s, 1H), 8.29 (d, / = 8.3 Hz, 1H), 7.77-7.70 (m, 1H),
7.41-7.30 (m, 3H), 6.95 (s, IK), 6.93-6.91 (m, 1H), 5.32-5.22 (m, 1H), 2.36 (s, 3H), 1.5M.45 (m, 5H), 1.15-1.01 (m, 2H).

To a solution of n-BuLi (2.5M in hexanes, 41.8niL, 0.104 mol) in 400 mL THF at -78 °C was added 2,2,6,6-tetramethylpiperidine (14.76 g, 0.1045 mol) dropvvise followed by 3-bromochIorobenzene (20.00g, 0,1045 mol) dropwise. The mixture was stirred at -7S °C for 2h, then quenched with dry ice and warmed to room temperature. The solution was concentrated in vacuo and the resulting residue was dissolved in water and washed with ether. To the aqueous fraction was added IN HCl to pH = 2, and the product was extracted with CH2CI2. The combined organics were dried over Na2S04, filtered and concentrated under vacuum to provide 2-bromo-6-
chlorobenzoic acid that gave proton NMR spectra consistent with theory.
To a solution of 2-bromo-6-chlorobenzoic acid (17.7g, 75.2 ramol) in
methanol (200 mL) at 0° was added (trimethylsilyl)diazomethane (2M in hexanes,
100 mL, 0.200 mol). The solution was stirred at 0 °C for 1.5 hours, then warmed to
room temperature and washed with aqueous sodium bicarbonate and brine, dried over Na2S04, filtered and concentrated. The residue was subjected to silica gel
chromatography eluted with 0-5% ethyl acetate in hexanes to provide methyl 2-bromo-6-chlorobenzoate as a pale yellow oil that gave proton NMR spectra consistent with theory.
A mixture of methyl 2-bromo-6-chlorobenzoate (2.25g, 9.03 mmol), tert-butyl(lR)-l-[2-f]uoro-4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)phenyI]-ethylcarbonate (see Example 11, 3.00 g, 8.21 mmol), potassium carbonate (2.84 g, 20.5 mmol), tri-o-tolylphosphine (0.10 g, 0.33 mmol), and palladium acetate (0.018 g, 0.08 ramol) in 40 mL of THF and 4 mL of water was heated in a sealed flask at 100°C for 4h. The mixture was then cooled and concentrated in vacuo. The resulting residue was dissolved in ethyl acetate, washed with water and brine, dried over Na2S04, filtered and concentrated under vacuum. The residue was subjected to silica
gel chromatography eluted with 0-10% ethyl acetate and hexane to provide methyl 4'-{(lR)-l-[(tert-butoxycarbonyl)amino]ethyl}-3-chloro-3*-fluoro-I,r-biphenyl-2-carboxylate that gave proton NMR spectra consistent with theory. The product was dissolved in ethyl acetate and saturated with HCl gas. The solution was concentrated in vacuo and azeotroped 3x with toluene to provide (iR)-l-[3'-chloro-3-fluoro-2'-(methoxycarbonyI)-l,r-biphenyI-4-yl]ethanaminium chloride.
A solution of the above compound (i.OOg, 2.91 mmol), boc-1-amino-cyclopropane-1-carboxylic ac:d (0.614g, 3.05 mmol), l-ethyl-(3-dimethylamino-propyl)carbodiimide hydrochloride (l.Ug, 5.81 mmol), l-hydroxy-7-azabenzotriazole (0.010 g, 0.15 mmol), and trie±ylamine (1.76 g, 17.4 mmol) in 20 mL CH2CI2 was

stilted at room temperature overnight. The solution was washed with aqueous sodium bicarbonate and brine, dned over Na2S04, filtered and concentrated. The residue was
subjected to silica gel chromatography eluted with 10-40% ethyl acetate in hexanes to
provide methyl 4-((lR)-i-[({ l-[(tert-buto\ycarbonyl)aminojcyclopropyl)carbonyl)-
amino]ethyl}-3-chloro-3'-fiuoro-l,1-biphenyl-2-carboxylate as a white solid that gave
proton N?vIR spectra consistent with theory. The product was dissolved in ethyl
acetate and saturated with HCl gas. The solution was concentrated in vacuo and
azeotroped three times wiih toluene to provide l-[({(lR)-l-[3'-chloro-3-fluoro-2-
(methoxycarbonyl)-l,r-biphenyl-4-yl]ethyl}amino)carbonyI]cyclopropanaminium
chloride that gave a mass ion (ES+) of 391.21 for M+H+.
To a solution of the above compound (0.300 g, 0.702 mmol) in 5 mL CH2CI2 at 0 °C was added triethylamine (0.142 g, 1.40 mmol) and trifluoroacetic
anhydride (0.147 g, 0.70 mmol). The solution was diluted with addition CHoCh and
washed with aqueous sodium bicarbonate and brine, dried over Na2S04, filtered and
concentrated. The residue was subjected to silica gel chromatography eluted with 10-40% ethyl acetate in hexanes to provide the title compound that gave proton NMR spectra consistent with theory and a mass ion (ES+) of 487.22 for M+H+: iH NMR (300 MHz, MeOH-d4) 5 7.51-7.33 (m, 4H), 7.17-7.07 (m, 2H), 5.31 (q, 7 = 7.1 Hz,
1H), 3.69 (s, 3H), 1.52-1.49 (m, 5H), 1.27-1.03 (m, 2H).
EXAMPLE 6
N-{(lR)-l-[33'-difluoro-2H2-methyl-2H-tetrazol-5-yl)-l,r-biphenyl-4-yl]ethyl}-l-[(trifluoroacetyl)amino]cyclopropanecarboxanude

A solution of 2-fluoro-6-iociobenzonitrile (17.82g, 72.15 mmol) and
azidotrinnethylcin (15.OOg, 72.88 mmol) in 150 mL toluene was heated to 125 °C for
72 hours. The solution u-as cooled to room temperature and partitioned between ethyl
acetate and 0.5 N HCl. The organic extract was washed with water and brine, dried overNa2S04, filtered and concentrated under vacuum to provide 5-(2-fluoro-6-iodo-
phenyl)-lH-tetrazole that gave proton NMR spectra consistent with theory and a mass ion (ES+) of 291.01 for M+H+.
A mixture of 5-(2-fluoro-6-iodophenyI)-lH-tetra2ole (23.48g, 80.97 mmol), potassium carbonate (16.79g, 0.121 mol), and iodomethane (16.09g, 0.113 mol) in 25 mL DIVEF was stirred at room temperature for 3 hours. The mixture was
partitioned between ethyl acetate and water, and the organic extract was washed with water and brine, dried over Na2S04, filtered and concentrated under vacuum. The
residue was subjected to silica gel chromatography eluted with 0-10% ethyl acetate in
hexanes to provide 5-(2-fluoro-6-iodophenyl)-2-methyl-2H-tetrazole that gave proton
NMR spectra consistent with theory and a mass ion (ES+) of 305.06 for M+H+.
To a solution of 2-fluoro-4-bromo-benzaldehyde (20.0 g, 98.5 mmol) in 500 mL of THF at 0 °C was added slowly a solution methyllithium (1.4 M in Et20,
70.3 mL). After one hour the reaction was carefully quenched with saturated aqueous ammonium chloride, extracted with diethyl ether, washed with saturated NaCl, dried over MgS04^ and concentrated. A solution of the crude alcohol in 200 mL of
CH2CI2 and TEA (14.0 "mL, 100 mmol) at 0 °C was added methanesulfonyl chloride
(7.42 mL, 95.9 mmol) over a period of five minutes. After and overnight reaction period, the reaction was extracted with CH2CI2 , washed with NaHC03, saturated NaCI, dried over MgS04 , and concentrated. A solution of the crude mesylate in 50
mL of DMF was treated with sodium azide (12.5 g, 191 mmol) overnight. The reaction was extracted with EtOAc , washed with NaHCOs, saturated NaCl/dried
over MgS04 . and concentrated. To a solution of the crude azide in 100 mL of THF was triethylphosphine (1 M in THF, 90.1 mL) over a period of 30 minutes. After one hour, 100 mL of 1 N HCl was added and the mixture was heated overnight. The mixture was cooled and washed with Et20. The aqueous layer was made basic by the
addition of potassium carbonate, extracted with Et20, dried over MgS04 , and concentrated. A solution of the crude amine (9.0 g, 41.2 mmol) in 50 mL of CH2CI2 was treated with Boc anhydride (9.00 g, 41.2 mmol). After one hour the mixture was concentrated. A solution of the crude carbamate in 40 mL of DMSO was added potassium acetate (12.1 g, 123.1 mmol), bis(pinacolato)diboron (11.5 g, 45.4 mmol),

and dichloro[l,r-bis(diphenylphosphino)ferrocene]pa!ladiuni (H) dichloromethane adduct (1.4 g, 1.8 mmol). The resulting mixture was heated at 80 °C for 16 hours. After cooling to room temperature, the mixture was partitioned between water and EtOAc. The organic extrac: was washed with brine, dried over MgS04, filtered and
concentrated under vacuum. The residue was subjected to silica gel chromatography
eluted with 20% ethyl acetace in hexanes to provide tert-butyl i-[2-fluoro-4-(4,4,5,5-
tetramethyl-l,3,2-dioxaboroian-2-yl)phenyl]ethylcarbamate as a tacky solid that gave
a proton NMR consistent with theory.
A mixture of 5-(2-fluoro-6-iodophenyl)-2-methyI-2H-tetra2ole (1.49g,
4.89 mmol), tert-butyl l-[2-riuoro-4-(4,4,5,5-tetramethyI-l,3,2-dioxaborolan-2-yl)-
phenyl]ethylcarbamate (l-70g, 4.65 mmol), potassium carbonate (1.61 g, 11.6 mmol),
tri-o-tolylphosphine (0.057 g, 0.19 mmol), and palladium acetate (0.010 g, 0.05
mmol) in 20 mL of THF and 4 mL of water was heated in a sealed flask at 100 °C
overnight. The mixture was then cooled and concentrated in vacuo. The resulting
residue was dissolved in ethyl acetate, washed with water and brine, dried over Na2S04, filtered and concentrated under vacuum. The residue was subjected to silica
gel chromatography eluted with 0-20% ethyl acetate and hexane to provide tert-butyl l-[3,3'-difluoro-2'-(2-methyI-2H-tetrazol-5-yl)-l,1-biphenyl-4-yl]ethyIcarbamate as a yellow oil that gave proton NMR spectra consistent with theory. The product was dissolved in ethyl acetate and saturated with HCl gas. The solution was concentrated in vacuo and azeotroped 3x with toluene to provide l-[3,3'-difluoro-2'-(2-methyl-2H-tetra2ol-5-yl)-l, r-biphenyl-4-yI]ethanaminium chloride.
A solution of l-[3,3'-difluoro-2'-(2-methyl-2H-tetra2ol-5-yl)-l,r" biphenyl-4-yl]ethanaminium chloride (0.670g, 1.89 mmol), Boc-1-aminocyclo-propane-1-carboxylic acid (0.419g, 2.08 mmol), l-ethyl-(3-dimethylaminopfopyl)-carbodiimide carboxylic acid (0.726g, 3.79 mmol), l-hydroxy-T-azabenzotriazoIe (O.OlOg, 0.15 mmol), and triethylamine (1.15g, 11.4 mmol) in 10 mL CH2CI2 was
stirred at room temperature for 48 hours. The solution was washed with aqueous sodium bicarbonate and brine, dried over Na2S04, filtered and concentrated. The
residue was subjected to silica gel chromatography eluted with 10-50% ethyl acetate in hexanes to provide tert-butyl !-[({l-[3,3'-difluoro-2'-(2-methyl-2H-tetra2ol-5-yl)-l,r-biphenyl-4-yl]ethyl}amino)carbonyl]cyclopropylcarbamate that gave proton NMR spectra consistent with theory and a mass ion (ES+) of 499.7 for M+H+. The enantiomers were resolved at this point on a Chiralcel OD column 10% isopropanol/

hexane (containing 0.1% TFA). The R enantiomer elated first and was used for the remainder of the synthesis.
The above product was dissolved in ethyl acetate and saturated with HCl gas. The solution was concentrated in vacuo and azeotrooed 3x with toluene to provide (lR)l-[((l-[3,3'-d!fluoro-2-(2-methyl-2H-tetrazol-5-yl)-l,l-biphenyl-4-yl]-ethyl }amino)carbonyl]cyclcpropanaminium chloride that gave a mass ion (ES-f) of 399.6 for M-+H+.
To a solution of the above compound (0.105g, 0.241 mmol) in 2 mL CH2CI2 at 0 °C was added triethylamine (0.049g, 0.48 mmol) and trifluoroacetic anhydride (0.05Ig, 0.24 mmol). The solution was diluted with additional CH2CI2 and washed with aqueous sodium bicarbonate and brine, dried over Na2S04, filtered
and concentrated. The residue was subjected to silica gel chromatography eluted with
10-40% ethyl acetate in hexanes to provide the title compound that gave proton NTVIR
spectra consistent with theory and a mass ion (ES+) of 495.32 for M+H+: 1H NMR (300 MHz, MeOH-d4) 5 7.6S-7.61 (m, 1H), 7.34-7.21 (m, 3H), 6.89-6.83 (m, 2H),
5.23 (d. / = 7.1 Hz, 1H), 4.86 (s, 3H), 1.49 (d, J = 3.7 Hz, 2H), 1.44 (d, 7 = 7.I Hz, 3H), 1.18-101(m,2H).

The following compounds in Table 2 were prepared by methods analogous to those described in Example 1.

The following compounds in Table 4 were prepared by methods analogous to those described in Example 1.

The following compounds in Table 5 were prepared by methods analogous to those described in Example 2.

The following compounds in Table 7 were prepared by methods analogous to those described in Example 1.

The following compounds in Table 9 were prepared by methods analogous to those described in Example 3, using the commercially available l-[(tert-butoxycarbonyl)amino]cyclobutanecarboxylic acid instead of l-[(tert-butoxy-carbonyl)amino]cyclopropanecarboxylic acid.

The following compounds in Table 10 were prepared by methods analogous to those described in Example 1, using the commercially available l-[(tert-butoxycarbonyl)amino]cyclobutanecarboxylic acid instead of l-[(tert-butoxy-carbonyl)amino]cyclopropanecarboxylic acid.

New Claims
WHAT IS CLAIMED 15:
I. A compound having the formula 1(3) and pharmaceutically
Acceptable salts thereof:

wherein m is 0 or 1. R6a 2-niethy!-2H-tetrazol-5-yL 3-melhyl-l,2,4-oxadiazol-5-yl. C02R or C(0)NHOR. harem Ra is alkyl; R6b is hydrogen, fluorine or chlorine; R3b is alky is selected from alkyl optionally substituted with 1 to 5 halogen atoms or a cyano group, C3-6 isoxazolyl, pyrimidinyl
and pyridines (and N-oxide thereof) optionally substituted with halogen or trifluoromethyl; and Ram is hydrogen or fluorine.

3. A compound of Claim 1 selected from;
methyl 3-fluoro-4'-{(lR)-I-[({l-[(3,3,3-trif]uoropropanoyl)amino]cyclopropyl}-carbonyl)amino]ethyl} -1,1 '-biphenyl-2-carboxylate,
3-fluoro-N-methoxy-4'-{(lR)-l-[({l-[(3,33-trifluoropropanoyl)amino.]cycIopropyl}-
carbonyl)amino]ethyl} -1,1- '-biphenyl-2carboxamide,
methyl 3-f]uoro-4'-{1 -methyl-1 -[({1 -[(trifluoroacer\1)amino]cyclopropyl}-
carbonyl)amino]ethyl } -1,1 '-biphenyl-2-carboxylate,
N-{(lR)-l-[3,3'-difluoro-2X3-methyl4,2,4.oxadiazoI-5-yl)-l,r.biphenyl-4-
yl]ethyl}-I-[(trifluoroacetyl)amino]cyclopropanecarboxamide,
methyl 3-chIoro-3'-fluoro-4*- {(1R)-1 -[({1 -[(trifluoroacetyl)amino]cyclopropyl} -
carbonyl)amino]ethyl}-1,1 '-biphenyl-2-carboxylate, and
N-{(lR)-l-[3,3'-difluoro-2X2-methyl-2H4etrazolo'-yl)-iphenyI-4-yI]eth[(trifluoroacetyl)amino]cyclopropanecarboxamide;
and pharmaceutically acceptable salts thereof
4. The compound methyl 3-chloro-3'-fluoro-4'-{(lR)-l-[({l-[(trifluoroacetyl)amino]cyclopropyl} carbonyl)amino]ethyl} -1,1 '-biphenyl-2-carboxylate.
5. A pharmaceutical composition comprising a therapeutically effective amount of a compound of Claim 1 and pharmaceutically acceptable recipients.

6. Use of a compound of Claim 1 or a pharmaceuiically acceptable salt thereof in the manufacture of a medicament for the treatment or prevention of pain and inflammation.
7. Use of a comopound of Claim 4 for the manufacture of a medicament for the treatment and prevention of pain and inflammation.
8. The use of Claim 6 wherein said pain is postherpetic neuralgia, osteoarthrititis pain, post- or perioperative pain or dental pain.
9. The use of Claim 7 wherein said pain is postherpetic neuralgia, osteoarthrititis pain, post- or perioperative pain or dental pain.

10. A compound having the formula 1(3) and pharmaceutically acceptable salts thereof, substantially as herein described herein and extemporized.

Documents:

1753-chenp-2004-abstract.pdf

1753-chenp-2004-claims filed.pdf

1753-chenp-2004-claims granted.pdf

1753-chenp-2004-correspondnece-others.pdf

1753-chenp-2004-correspondnece-po.pdf

1753-chenp-2004-description(complete)filed.pdf

1753-chenp-2004-description(complete)granted.pdf

1753-chenp-2004-form 1.pdf

1753-chenp-2004-form 26.pdf

1753-chenp-2004-form 3.pdf

1753-chenp-2004-form 5.pdf

1753-chenp-2004-other documents.pdf

1753-chenp-2004-pct.pdf

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Patent Number

212241

Indian Patent Application Number

1753/CHENP/2004

PG Journal Number

07/2008

Publication Date

15-Feb-2008

Grant Date

26-Nov-2007

Date of Filing

05-Aug-2004

Name of Patentee

M/S. MERCK & CO., INC

Applicant Address

126 East Lincoln Avenue, Rahway, NJ 07065-0907

Inventors:

#	Inventor's Name	Inventor's Address
1	WOOD, Michael, R	126 East Lincoln Avenue, Rahway, NJ 07065-0907

PCT International Classification Number

C07D 213/82

PCT International Application Number

PCT/US2003/003338

PCT International Filing date

2003-02-04

PCT Conventions:

#	PCT Application Number	Date of Convention	Priority Country
1	60/355,062	2002-02-08	U.S.A.