Indian Patents. 213049:N-ARYLSULFONYL AMINO ACID OMEGA AMIDES AND THE PROCESS FOR THEIR PREPARATION

Title of Invention	N-ARYLSULFONYL AMINO ACID OMEGA AMIDES AND THE PROCESS FOR THEIR PREPARATION
Abstract	The invention relates to compounds of formula (I) and/or a stereoisomeric form of the compound of formula (I) and/or a physiologically acceptable salt of the compound of formula (I) which are suitable for producing medicaments for the therapy and prophylaxis of diseases in whose course matrix-degrading metalloproteinases are involved.

Full Text	Description N-Arylsulfonylamino acid omega-amides The invention relates to novel N-aryisulfonylamino acid omega-amides, processes for their preparation, and use thereof as pharmaceuticals. The applications EP 0 606 046, WO 95/35276 and WO 96/27583 describe arylsulfonaminohydroxamic acids and their action as matrix metalloproteinase inhibitors. Specific arylsulfonamidocarboxylic acids are used as intermediates for the preparation of thrombin inhibitors (EP 0 468 231) and aldose reductase inhibitors (EP 0 305 947). The application EP 0 757 037 also describes the action of sulfonylamino acid derivatives as metalloproteinase inhibitors. In the effort to find efficacious compounds for the treatment of connective tissue disorders, it has now been found that the sulfonylaminocarboxylic acids according to the invention are strong inhibitors of matrix metalloproteinases. Particular value is placed here on the inhibition of stromelysin (MMP-3) and neutrophii collagenase (MMP-8), as both enzymes are decisively involved in the degradation of the proteoglycans, as important constituents of the cartilaginous tissue (A. J. Fosang et al. J. Clin. Invest. 98 (1996) 2292-2299). Those enzymes which are involved in the constitutive degradation and synthesis of matrix constituents likewise belong to the protein family of the matrix metalloproteinases. For example, MMP-1 (collagenase -1) has an important vital function, since it is involved in natural collagen degradation, in particular even where morphogenetic changes take place. Medicinal active compounds which, although they are able to inhibit MMP-3 and MMP-8, at the same time leave MMP-1 largely unaffected, are thus preferred. Such an active compound can even be particularly preferred with respect to the healing of the human or animal body, which, with, all in all, only moderate inhibition of MMP-3 and -8, shows no or a weaker effect on the MMP-1. and/or a stereoisomeric form of the compound of the formula I and/or a physiologically tolerable salt of the compound of the formula I, where R is 1. phenyl, 2. phenyl, which is mono- or disubstituted by 2.1. (C-i-C6)-alkyl, which is linear, cyclic or branched, 2.2. hydroxyl, 2.3. (Ci-C6)-alkyl-C(0)-0-, 2.4. (Ci-C6)-alkyl-O-, 2.5. (Ci-Ce)-alkyl-0-(Ci-C4)-alkyl-0, 2.6. halogen, 2.7. -CF3, 2.8. -CN, 2.9. -N02, 2.10. HO-C(O)-, 2.11. (Ci-C6)-alkyl-0-C(0)-, 2.12. methylenedioxo, 2.13. R4-(R5 )N-C(O)-, 2.14. R4-(R5)N-,or 2.15. a heterocycle from the following group: isoxazolidine, morpholine, isothiazolidine, thiomorpholine, pyrazolidine, imidazolidine, piperazine, azetidine, pyrrole, pyrroline, pyrrolidine, pyridine, azepine, piperidine, oxazole, isoxazole, imidazole, pyrazole, thiazole, isothiazole, diazepine, thiomorpholine, pyrimidine and pyrazine, which is unsubstituted or substituted as described under 2.1 to 2.14, or 3. a heteroaromatic from the following group 3.1. to 3.15., which is unsubstituted or substituted as described under 2.1 to 2.14, 3.1. pyrrole, 3.2. pyrazole, 3.3. imidazole, 3.4. triazole, 3.5. thiophene, 3.6. thiazole, 3.7. oxazole, 3.8. isoxazole, 3.9. pyridine, 3.10. pyrimidine, 3.11. indole, 3.12. benzothiophene, 3.13. benzimidazole, 3.14. benzoxazole or 3.15. benzothiazole, 2 4 5 R , R and R are identical or different and are 1. a hydrogen atom, 2. (Ci-Ce)-alkyh 3. HO-C(OMCi-C6)-alkyl-, 4. phenyl-(CH2)m in which phenyl is unsubstituted or mono- or disubstituted as described under 2.1. to 2.14. and n is the integer zero, 1 or 2, or 5. picolyl or 6. R and R , together with the ring amino group, form a 4- to 7-membered ring in which, if appropriate, one of the carbon atoms is replaced by -0-, -S- or -NH-, R3is1. -(Ci-C4)-alkyl-C(0)-N(R6)-R7, in which R6 and R7 together with the nitrogen to which they are bonded form a radical of the subformula lla, Mb or lie where in the subformulae lla, lib and lie q is the integer zero, 1 or 2 and r is the integer zero or 1, Z is a carbon, nitrogen, oxygen or sulfur atom or a covalent bond, and Q R is a hydrogen atom or has the meaning described for R1 above under 2.1 to 2.14, 2. -(Ci-C4)-alkyl-C(O)-Y, in which Y is a radical of the subformula lie or lid where in the subformulae lie and lid o R is a hydrogen atom or has the meaning described for R above under 2.1 to 2.14 and g R is 2.1 a hydrogen atom 2.2 (Ci-C6)-alkyl-, 2.3 HO-C(0)-(Ci-C6)-alkyl-, 2.4 phenyl-(CH2)rr, in which phenyl is unsubstituted or mono- or disubstituted 1 as described for R under 2.1 to 2.14 and n is the integer zero, 1 or 2, or 2.5 picolyl, or 3. •(Ci-C4)-alkyl-C(O)-N(R^-(CH2)o-N(R4)^5 , where g R has the abovementioned meaning, 0 is the integer 2, 3, 4 or 5 and 4 5 R and R have the abovementioned meaning, A is a) a covalent bond, b) -0-, c) -CH=CH- or d) -OC-, B is a) -(CH2)m-1 in which m is the integer zero, 1,2,3,4,5 or 6, b) -O-(CH2)p, in which p is an integer from 1 to 5, or c) -CH=CH- and X is -CH=CH-, an oxygen atom or a sulfur atom. A compound of the formula I is preferred, where R is 1. phenyl or 2. phenyl, which is monosubstituted by 2.1. (Ci-Ce)-alkyl-, in which alkyl is linear, cyclic or branched, 2.2. -OH, 2.3. . (Ci-Ce)-alkyl-C(0)-0-, 2.4. (Ci-C6)-alkyl-0-, 2.5. (C1-C6)-alkyl-0-(C1-C4)-alkyl-O, 2.6. halogen, 2.7. -CF3 or 2.8. R4-(R5 )N-, 2 4 5 R , R and R are identical or different and are 1. a hydrogen atom, 2. (d-C6)-alkyl-or 4 5 3. R and R together with the ring amino group form a 4- to 7-membered ring, in which, if appropriate, one of the carbon atoms is replaced by -0-, -S- or -NH-, R3is 1. -(Ci-C4)-alkyl-C(0)-N(R6)-R7i in which R6 and R7 together with the nitrogen to which they are bonded form a radical of the subformula Ha, where in the subformula lla q is the integer zero or 1, Z is a carbon, nitrogen, oxygen or sulfur atom, and Q R is a hydrogen atom or has the meaning described for 1 R above under 2.1 to 2.8, 2, -(Ci-C4)-alkyt-C(0)-Y, in which Y is a radical of the subformula lie or lid where in the subformulae lie and lid o R is a hydrogen atom or has the meaning described for R above under 2.1 to 2.8 and g R is a hydrogen atom, or 3. -(Ci-C4)-alkyl-C(0).N(R9)-(CH2)0-N(R4)-R5 , where g R is a hydrogen atom, o is the integer 2 or 3 and 4 5 R and R are identical or different and are 1. a hydrogen atom, 2. (C1-C6)-alkyl-or 4 5 3. R and R together with the ring amino group form a 4- to 7-membered ring, in which, if appropriate, one of the carbon atoms is replaced by -0-, -S- or -NH-,. A is a covalent bond or -0-, B is a) -(CH2)m-. in which m is the integer zero, 1 or 2, or b) -O-(CH2)p, in which p is the integer 1 or 2, and X is -CH=CH-. A compound of the formula I is furthermore preferred, where R is 1. phenyl or 2. phenyl, which is monosubstituted by 2.1. chlorine, 2.2. bromine, 2.3. fluorine, 2.4. pyrrolidine or 2.5. morpholine, R is a hydrogen atom, R3is 1. -(Ci-C4)-alky\|.C(0)-N(R6)-R7» in which R6 and R7 together with the nitrogen to which they are bonded form a radical of the subformula I la, where in the subformula Ha q is the integer zero or 1, Z is a carbon atom, and o R is a hydrogen atom, chlorine, bromine or fluorine, 2. -(Ci-C4)-alkyl-C(O)-Y, in which Y is a radical of the subformula lie or lid where in the subformulae lie and lid 8 9 R and R are each a hydrogen atom, or 3. -(Ci-C4)"alkyl-C(0)-N(R9HCH2)o-N(R4)-R5, where g R is a hydrogen atom, o is the integer 2 or 3 and 4 5 R and R are identical or different and independently of one another are 1. a hydrogen atom, 2. phenyl or 3. morpholine, A is a covalent bond or -0-, B is a covalent bond and X is -CH=CH-. The compounds 2-(biphenyl-4-sulfonylamino)-4-(naphthalen-1-ylcarb-amoyl)butyric acid, 2-(biphenyl-4-sulfonylamino)-4-(naphthalen-2-yl-carbamoyl)butyric acid, 2-(biphenyl-4-sulfonylamino)-4-(2-phenylamino- ethylcarbamoyl)butyric acid, 2-(4'-chloro-biphenyl-4-sulfonylamino)-4-(3-morpholin-4-ylpropylcarbamoyl)butyric acid, 4-(3-(4-(biphenyl-4-sulfonyl-amino)-4-carboxybutyrylamino)propyl)morpholin-4-ium trifluoroacetate, 2-(biphenyl-4-sulfonylamino)-5-(2,3-dihydroindol-1-yl)-5-oxopentanoic acid, 5-(2,3-dihydroindol-1-yl)-5-oxo-2-(4'-pyrroiidin-1-yl-biphenyl-4-sulfonyl-amino)pentanoic acid, 2-(4'-chlorobiphenyl-4-sulfonylamino)-5-(2,3-dihydroindol-1-yl)-5-oxopentanoic acid, 2-(4'-bromobiphenyl-4-sulfonyl-amino)-5-(2,3-dihydroindoM-yl)-5-oxopentanoic acid, 2-(4'-chloro-biphenyl-4-sulfonylamino)-5-(5-fluoro-2,3-dihydroindol-1-yl)-5-oxopentanoic acid, 2-(4'-bromobiphenyl-4-suifonylamino)-5-(5-fluoro-2,3-dihydroindoi-1-yl)-5-oxopentanoic acid, 5-(5-fluoro-2,3-dihydroindol-1 -yl)-5-oxo-2-(4'-pyrrolidin-1-ylbiphenyl-4-sulfonylamino)pentanoic acid or 5-(5-fiuoro-2,3-dihydroindol-l-yl)-2-(4'-morpholin^-yl-biphenyM-suifonylamino)-5•oxopentanoic acid are particularly preferred. 4 5 The expression " R and R together with the ring amino group form a 4- to 7-membered ring and/or one of the carbon atoms is replaced by -0-, -S- or -NH-" is understood as meaning radicals which are derived, for example, from isoxazolidine, morpholine, isothiazolidine, thiomorpholine, pyrazolidine, imidazolidine, piperazine, azetidine, pyrrole, pyrroline, pyrrolidine, pyridine, azepine, piperidine, pyrazole or diazepine. The term "halogen" is understood as meaning fluorine, chlorine, bromine or iodine. The term "alky!" is understood as meaning hydrocarbon radicals whose carbon chain is linear or branched. The starting substances of the chemical reactions are known or can easily be prepared by methods known from the literature. The invention furthermore relates to a process for the preparation of the compound of the formula I and/or of a stereoisomeric form of the compound of the formula I and/or of a physiologically tolerable salt of the compound of the formula I, which comprises (as described in method A, see examples) 11 in which R# is a hydrogen atom or an ester protective group which is customary in peptide chemistry, s is the integer zero, 1, 2 or 3, R is the radical -OR , in which R is an ester protective group, 11 which can be cleaved in the presence of R , or a hydrogen atom, or R is a radical -N(R )-R , in which R and R are as defined in formula I, with a sulfonic acid derivative of the formula III where R ,X,A and B are as defined in formula I and Y is a halogen 13 13 atom, imidazolyl or OR , in which R is a hydrogen atom, (C‹i-C6)-alkyl, phenyl or benzyl, which is optionally substituted, in the presence of a base or, if appropriate, of a dehydrating agent to give a compound of the formula IV in which R , A, X, B, R , s, R and R have the abovementioned 12 meaning, and then removing a protective group R possibly included in the radical R10 in the presence of the protective group R , and then introducing the radical -N(R )-R by appropriate activation, known from peptide chemistry, of the carboxyl group, and 11 removing the included protective group R and obtaining the compound of the formula I, or (as described in methods B and C, see examples) i) reacting an amino acid anhydride of the formula V 13 14 in which R is a hydrogen atom and R is an N-protective group known from peptide chemistry, such as, for example, carbobenzyloxy (Z), or R and R are a cyclic N-protective group, e.g. phthalimido, and s is as defined above, with a primary or secondary radical -N(R )-R in the sense of a ring opening to give an intermediate of the formula VI in which R , R , s, R and R have the abovementioned meaning, where this ring opening, depending on the protective group and reaction conditions (cf. X. Huang, X. Luo, Y. Roupioz, J. W. Keillor, J. Org. Chem. 1997, 62, 8821-8825) as a rule regioselectively yields the isomer shown in formula VI, which, if regioisomer mixtures occur, can be further enriched by crystallization or chromatography, then cleaving the included protective group R and/or R with release of the amine, and then carrying out, as described under a) an N-sulfonation with a sulfonic acid derivative of the formula III which leads to a product of the formula I, or c) resolving a compound of the formula I prepared according to process a) or b), which on account of its chemical structure occurs in enantiomeric forms, into the pure enantiomers by salt formation with enantiomerically pure acids or bases, chromatography on chiral stationary phases or derivatization by means of chiral enantiomerically pure compounds such as amino acids, separation of the diastereomers thus obtained, and removal of the chiral auxiliary groups, or d) isolating the compound of the formula I prepared according to process a), b) or c) in free form or, if acidic or basic groups are present, converting it into physiologically tolerable salts. Suitable protective groups for this are preferably the customary N-protective groups used in peptide chemistry, for example protective groups of the urethane type, benzyloxycarbonyl (Z), t-butyloxycarbonyl (Boc), 9-fluorenyloxycarbonyl (Fmoc), allyloxycarbonyl (Alloc) or of the acid amide type, in particular formyl, acetyl or trifluoroacetyl, and of the alkyl type, for example benzyl. Starting materials used for the preparation of the sulfonic acid derivatives of the formula III are preferably sulfonic acids or their salts of the formula VII, for example 15 1 where R is a radical described for R under 2.1. to 2.14. 15 Sulfonic acid derivatives of the formula III, which like R contain a 4 5 secondary or cyclic amine of the type -N(R )-R , are prepared preferably and in high yields by Pd-catalyzed substitution of a bisaryl halide, preferably of a bromide, by a secondary amine following known literature procedures (cf. A.S. Guram, R.A. Rennels, S.L Buchwald; Angew. Chem. 1995, 107, 1456-1459) and subsequent sulfochlorination by means of chlorosulfonic acid. The sulfochloride function is in this case preferably guided into the desired para position by the directing effect of the amine group. The catalyst dichlorobis(tritolylphosphine)palladium(ll) advantageously used can be prepared analogously to R.F. Heck in "Palladium Reagents in Organic Syntheses", Academic Press, London (1985), p. 18 starting from tri-o-tolylphosphine, palladium(ll) chloride and LiCI in methanol. For the preparation of the arylsulfonic acids of the formulae Vila and Vllb, the sulfonation process with concentrated sulfuric acid described in Houben-Weyl "Methoden der Organischen Chemie" [Methods of Organic Chemistry] volume 9, pp. 540-546 is preferably used, if appropriate in the presence of a catalyst, sulfur trioxide and its addition compounds or halosulfonic acids, such as chlorosulfonic acid. Particularly in the case of the diphenyl ethers of the formula Vllb, the use of concentrated sulfuric acid and acetic anhydride as a solvent (cf. CM. Suter, J. Am. Chem. Soc. 53 (1931) 1114), or the reaction with excess chlorosulfonic acid (J.P. Bassin, R. Cremlyn and F. Swinbourne; Phosphorus, Sulfur and Silicon 72 (1992) 157) has proven suitable. Sulfonic acids according to the formulae Vile, Vlld or Vile can be prepared in a manner known per se by reacting the appropriate arylalkyl halide with sulfites such as sodium sulfite or ammonium sulfite in aqueous or aqueous/alcoholic solution, it being possible to accelerate the reaction in the presence of tetraorganoammonium salts such as tetrabutylammonium chloride. Sulfonic acid derivatives according to formula III used, are, in particular, the suifonyl chlorides. For their preparation, the corresponding sulfonic acids, also in the form of their salts such as sodium, ammonium or pyridinium salts, are reacted in a known manner with phosphorus pentachloride or thionyl chloride without or in the presence of a solvent such as phosphorus oxychloride or of an inert solvent such as methylene chloride, cyclohexane or chloroform, in general at reaction temperatures of 2O°C up to the boiling point of the reaction medium used. Advantageously, the direct sulfochlorination of the appropriate aromatic can also be carried out using chlorosulfonic acid, sulfuryl chloride or pyrosulfuryl chloride (Houben-Weyl, Methoden der Organischen Chemie [Methods of Organic Chemistry], volume 9 (1995), pages 572 - 579). The reaction of the sulfonic acid derivatives of the formula III with the amino acids of the formula II according to process variants a) or b) proceeds advantageously in the style of the Schotten-Baumann reaction. Suitable bases for this are particularly alkali metal hydroxides such as sodium hydroxide, but also alkali metal acetates, alkali metal hydrogencarbonates, alkali metal carbonates and amines. The reaction takes place in water or in a water-miscible or nonmiscible solvent such as tetrahydrofuran (THF), acetone, dioxane or acetonitrile, the reaction temperature in general being kept at -10°C to 5O°C. In the case in which the reaction is carried out in anhydrous medium, tetrahydrofuran or methylene chloride, acetonitrile or dioxane in the presence of a base, such as triethylamine, N-methylmorpholine, N-ethyl- or diisopropylethylamine is especially used, optionally in the presence of N,N-dimethylaminopyridine as a catalyst. In another variant, particularly when using polar starting materials which are present in unprotected form, the aminocarboxylic acids of the formula II can first be converted into their silylated form with the aid of a silylating agent such as bis-trimethylsilyl trifluoroacetamide (BSTFA) and they then react with sulfonic acid derivatives to give compounds of the formula IV. The physiologically tolerable salts of the compounds of the formula I capable of salt formation, including their stereoisomeric forms, are prepared in a manner known per se. With basic reagents such as hydroxides, carbonates, hydrogencarbonates, alkoxides and ammonia or organic bases, for example 2-amino-2-hydroxymethyl-1,3-propanediol (tromethamine), trimethyl- or triethylamine, ethanolamine or triethanolamine or alternatively basic amino acids, for example lysine, ornithine or arginine, the carboxylic acids form stable alkali metal, alkaline earth metal or, if appropriate, substituted ammonium salts. Salts of the compound of the formula I which are formed with the organic bases mentioned show a high water solubility. If the compounds of the formula I have basic groups, stable acid addition salts can also be prepared using strong acids. For this, both inorganic and organic acids such as hydrochloric, hydrobromic, sulfuric, phosphoric, methanesulfonic, benzenesulfonic, p-toluenesulfonic, 4-bromobenzenesulfonic, acetic, oxalic, tartaric, trifluoromethylsulfonic, cyclohexylamidosulfonic, succinic or trifluoroacetic acid are suitable. The invention also relates to pharmaceuticals which contain an efficacious amount of at least one compound of the formula I and/or of a physiologically tolerable salt of the compound of the formula I and/or an optionally stereoisomeric form of the compound of the formula I, together with a pharmaceutically suitable and physiologically tolerable excipient, additive and/or other active compounds and auxiliaries. On account of the pharmacological properties, the compounds according to the invention are suitable for the prophylaxis and therapy of all those disorders in whose course matrix-degrading metalloproteinases are involved. These include degenerative joint disorders such as osteoarthroses, rheumatoid arthritis, spondyloses, chondrolysis after joint trauma or relatively long immobilization of the joint, e.g. after meniscus or patella injuries or tearing of the ligaments. In addition, these also include disorders of the connective tissue such as collagenoses, periodontal disorders, which can even lead to the loss of the teeth, wound healing disorders and chronic disorders of the locomotory apparatus such as inflammatory, immunologically or metabolically caused acute and chronic arthritis, arthropathies, myalgias and disorders of the bone metabolism (such as osteoporosis). The medicinal use of the compounds of the formula I according to the invention may be indicated in the case of vascular diseases, e.g. blood vessel occlusions, atherosclerotic plaques or aneurysms, particularly in threatening rupture, or in stenoses of any pathogenesis. The compounds of the formula I are furthermore suitable for the treatment of inflammations, including wounds and ulcers, in particular also those of the skin, carcinomatous disorders, in particular also for blocking or checking the formation and spread of metastases, and also in carcinoma of the breast. The treatment of cachexia, anorexia, septic shock, periodontosis and periodontitis are further medicinal areas of application for the compounds according to the invention. In general, the pharmaceuticals according to the invention are administered orally or parenterally. Rectal or transdermal administration is also possible. The invention also relates to a process for the production of a pharmaceutical, which comprises bringing at least one compound of the formula I into a suitable administration form using a pharmaceutically suitable and physiologically tolerable vehicle, and, if appropriate, further suitable active compounds, additives or auxiliaries. Suitable solid or pharmaceutical preparation forms are, for example, granules, powders, coated tablets, tablets, (micro)capsules, suppositories, syrups, juices, suspensions, emulsions, drops or injectable solutions as well as preparations with protracted release of active compound, in whose preparation customary auxiliaries, such as excipients, disintegrants, binders, coating agents, swelling agents, glidants or lubricants, flavorings, sweeteners and solubilizers are used. Frequently used auxiliaries which may be mentioned are magnesium carbonate, titanium dioxide, lactose, mannitol and other sugars, talc, lactoprotein, gelatin, starch, cellulose and its derivatives, animal and vegetable oils such as cod-liver oil, sunflower oil, peanut or sesame oil, polyethylene glycol and solvents such as, for example, sterile water and mono- or polyhydric alcohols such as glycerol. The pharmaceutical preparations are preferably prepared and administered in dose units, each unit containing as active constituent a specific dose of the compound of the formula I according to the invention. In the case of solid dose units such as tablets, capsules, coated tablets or suppositories, this dose can be up to approximately 1000 mg, but preferably approximately 50 to 300 mg and, in the case of injection solutions in ampoule form, up to approximately 300 mg, but preferably approximately 10 to 100 mg. For the treatment of an adult patient weighing approximately 70 kg, depending on the efficacy of the compounds according to formula I, daily doses of approximately 20 mg to 1000 mg of active compound, preferably approximately 100 mg to 500 mg, are indicated. Under certain circumstances, however, even higher or lower daily doses may be appropriate. The daily dose can be administered both by single administration in the form of an individual dose unit or else of several smaller dose units and by multiple administration of subdivided doses at intervals. The compounds on which the invention is based were identified by nuclear magnetic resonance and mass spectroscopy, where in the case of the presence of regio- or diastereoisomeric forms in addition to H also C and multidimensional NMR methods were employed for clear proof of structure. H NMR spectra have been recorded on a 400 MHz apparatus from Bruker, as a rule using tetramethylsilane (TMS) as an internal standard and at room temperature (RT). As a rule, final products are determined by mass spectroscopic methods (FAB-, ESI-MS with positive or negative ionization). Temperatures are in degrees Celsius, RT means room temperature (22°C to 26°C). Abbreviations used are either explained or correspond to the customary conventions. Preparation examples Method A) Example 5 4-(3-(4-(Biphenyl-4-sulfonylamino)-4-carboxybutyrylamino)- propyl)morpholin-4-iumtrifluoroacetate Stage 1: Sulfonation of glutamyl tert-butyl ester 3 g (0.0148 mol) of L-Glu-OtBu were dissolved in 29.5 ml of 0.5 N NaOH and 250 ml of tetrahydrofuran (THF) and cooled to 0°C in an ice bath, and simultaneously (while keeping the pH constant at pH 8.5), a solution of 4.476 g (0.0177 mol) of biphenylsulfonyl chloride in 35 ml of THF and 29.52 ml of an aqueous 0.5 N NaOH was slowly added dropwise over approximately 30 minutes (min). After removing the ice bath, the mixture was stirred at room temperature for 3 hours (h) and the completion of the reaction was monitored by thin-layer chromatography (TLC). THF was removed under reduced pressure, the residue was extracted once with diethyl ether, the extract was acidified to pH 1.5 using 1 N HCI, the product was extracted a number of times with ethyl acetate, the extract was dried and the solvent was removed under reduced pressure. 2.89 g of product remained, which could be reacted further without further purification. Stage 2: Conversion into the amide 0.25 g (0.596 mmol) of the product from stage 1 was dissolved in 50 ml of absolute THF, 0.119 g (0.63 mmol) of EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride) and 0.0845 g (0.55 mmol) of 1-hydroxy-benzotriazole hydrate were added, the mixture was stirred for 30 min, then 0.268 ml (1.79 mmol) of N-(3-aminopropyl)morpholine was added carefully, and the mixture was stirred for approximately 8 h. It was taken up with water, acidified using 1N HCI to pH 2, extracted a number of times with ethyl acetate, washed with dilute NaCI, dried and concentrated. Yield: 250 mg. A further purification could be carried out by chromatography on silica gel by means of dichloromethane/methanol 9:1. Stage 3: Removal of the protective group 0.11 g of the product from stage 2 was dissolved in 5 ml of trifluoroacetic acid and 5 ml of dichloromethane and the mixture was stirred at room temperature for approximately 3 h under protective gas until completion of the reaction (TLC checking). After concentration, the mixture was entrained a number of times with dichloromethane and the residue was dried under reduced pressure. 0.11 g of the compound of Example 5 remained as the trifluoroacetate. Method B) Examples 2-(Biphenyl-4-sulfonylamino)-5-(2,3-dihydroindol-1-yl)-5-oxo- pentanecarboxylic acid Stage 1: Reaction of phthaloyl-L-glutamyl anhydride with dihydroindole 1.5 g (0.0058 mol) of phthaloyl-L-glutamyl anhydride were dissolved in 15 ml absolute dioxane, and a solution of 0.813 ml (0.0073 mol) of dihydroindole in 15 ml of dioxane was added dropwise in the course of 10min. The mixture was heated until the reaction was complete (approximately 5 h, TLC checking) at 40 °C, concentrated, and the mixture was entrained a number of times under reduced pressure using dichloromethane. Yield: 2.7 g (product contained approximately 15 mol% of dioxane) Stage 2: Removal of the protective group 2.43 g of the product from stage 1 were dissolved in 30 ml of ethanol and 0.39 ml (0.0080 mol) of hydrazine hydrate was added. The mixture was stirred at 8O°C for 3 h, concentrated to dryness under reduced pressure, the residue was taken up using 50 ml of 25% strength aqueous acetic acid and the mixture was heated at 8O°C for approximately 10 min. It was then cooled in an ice bath and the resulting precipitate was filtered off with suction, the precipitate was taken up again using acetic acid and this procedure was repeated. The phthalyl hydrazide residue which remained was discarded. The combined filtrates were concentrated, and afforded 0.7 g of product. Stage 3: Introduction of the N-arylsulfonyl radical 0.5 g (0.002 mol) of the product from stage 2 was dissolved in 50 ml of THF/water (1:1) together with 0.45 g (0.0033 mol) of potassium carbonate, and a solution of 0.61 g (0.0024 mol) of biphenylsulfonyl chloride, dissolved in 50 ml of THF, was added dropwise in the course of 20 min. The mixture was stirred at RT until the reaction was complete (approximately 6 to 8 h, TLC checking), extracted once with diethyl ether and acidified to pH 1.5 using 1N HCI, and the product was extracted a number of times with ethyl acetate, dried and concentrated under reduced pressure. After drying, 0.42 g of the product of Example 6 remained. Method C) Example 8 2-(4'-Chlorobiphenyl-4-sulfonylamino)-5-(2,3-dihydroindol-l-yl)-5-oxopentanoic acid Stage 1: Reaction of Cbz-Glu anhydride with 2,3-dihydro-1H-indole 5 g (0.019 mol) of L-Cbz-glutamyl anhydride were dissolved in 100 ml of absolute dimethyl sulfoxide together with 2.56 ml (this corresponds to 0.023 mol) of 2,3-dihydro-1H-indole and the solution was stirred at room temperature (RT) for 30 min and at 4O°C for a further 30 min until the reaction was complete (TLC checking). The mixture was added to water, extracted with ethyl acetate a number of times, and the organic phase was washed with 1N HCI and with saturated NaCI solution, dried and concentrated. Yield: 7.07 g Stage 2: Removal of the Cbz protective group 6.8 g (0.018 mol) of the product from stage 1 were dissolved in 60 ml of a 33% strength HBr/glacial acetic acid solution in the course of 30 min and the solution was stirred at RT until the reaction was complete (about 15 h, TLC checking). The crude product obtained after concentration under reduced pressure was entrained a number of times with dichloromethane and taken up using methanol/water (10:1), and the product was filtered off, dried and again extracted by stirring in THF. After filtering off with suction and drying under reduced pressure, 2.13 g of the crystalline hydrobromide having a purity of 98% (according to HPLC) remained. The reductive removal of the protective group analogously to Example 12, stage 4, afforded the hydrochloride of 2-amino-5-(2,3-dihydroindoM-yl)-5-oxopentanoic acid in 85% yield. Stage 3: 2-(4'-Chlorobiphenyl-4-sulfonylamino)-5-(2,3-dihydroindol-1- yl)-5-oxopentanoic acid 0.33 g (1 mmol) of the product from stage 2 was dissolved in 60 ml of THF/water (1:1) and a pH of 12.0 was established using a titroprocessor and 0.5 N NaOH. Under constant pH, a solution of 0.345 g (1.2 mmol) of 4'-chlorobiphenyl-4-sulfonyl chloride, dissolved in 30 ml of THF, was added dropwise in the course of approximately 45 min. The mixture was stirred for a further 2 h at pH 12, (TLC checking), treated with 0.1 N HCI to pH 2, extracted a number of times with ethyl acetate, washed with water, dried and concentrated. Yield: 0.395 g of melting point 182°C and a purity of more than 92%. The 4-chlorobiphenylsulfonic acid detectable as a slight impurity could be removed by chromatography or reprecipitation. Example 12 5-(5-Fluoro-2,3-dihydroindol-l-yl)-5-oxo-2-(4'-pyrrolidin-l-yl-biphenyl-4-sulfonylamino) pentanoic acid Stage 1: Biphenyl-4-ylpyrrolidine 10.0 g (0.043 mol) of 4-bromobiphenyl were suspended in 600 ml of toluene together with 3.7 g (0.052 mol) of pyrrolidine and 6.2 g (0.0642 mol) of sodium tertiary-butoxide and 900 mg of the catalyst dichlorobis(tritolylphosphine)palladium(ll) were added. The mixture was heated to reflux for 8 h, cooled, treated with water/ethyl acetate, and the organic phase was washed with water, dried over sodium sulfate and concentrated. The residue was dissolved in approximately 300 ml of tertiary-butyl methyl ether and the hydrochloride of the product was precipitated by addition of 50 ml of 1N ethereal HCI. Yield: 6.0 g of melting point higher than 125°C (decomposition) Stage 2: 4'-Pyrrolidin-1-ylbiphenyl-4-suIfonyl chloride 6 g (0.023 mol) of the product from stage 1 was introduced into 11 ml of chlorosulfonic acid in portions with cooling and under protective gas and the mixture was heated at 6O°C for 3.5 h. The solution was added to ice, the suspension was brought to pH 8 by addition of solid sodium bicarbonate, and the precipitated product was filtered off with suction and dried under reduced pressure. Yield: 7 g of melting point higher than 282 °C (decomposition). Stage 3: Reaction of Z-Glu anhydride with 5-fIuro-2,3-dihydro-1H- indole 8.8 g (0.0334 mol) of L-Z-glutamyl anhydride were dissolved in 150 ml of absolute dimethyl sulfoxide together with 5.5 g (0.04 mol) of 5-fluoro-2,3- dihydro-1H-indole and the solution was stirred at RT until the reaction was complete (approximately 3 h, TLC checking). The mixture was added to water, extracted a number of times with ethyl acetate and the organic phase was washed with 1N HCI and with saturated NaCI, dried and concentrated. Yield: 13.2 g Stage 4: Removal of the Z protective group 13 g (0,0325 mol) of the product from stage 3 were dissolved in 450 ml of methanol and 33 ml of 1N HCI, a spatula tipful of palladium on carbon was added, and the mixture was hydrogenated at 40 bar and 6O°C until the reaction was complete (TLC checking). The crude product obtained after filtration and concentration was taken up in THF under reflux, and the solid hydrochloride which remained after cooling was filtered off and dried under reduced pressure. Yield: 7.1 g of melting point higher than 212°C Stage 5: 5-(5-Fluoro-2,3-dihydroindol-1-yl)-5-oxo-2-(4'-pyrrolidin-1- ylbiphenyl-4-sulfonylamino) pentanoic acid 0.303 g (1 mmol) of the product from stage 4 was dissolved in 60 ml of THF/water (1:1) and a pH of 12.0 was established using a titroprocessor (model 686, Metrohm) by means of 0.5 N NaOH. While keeping the pH constant, a solution of 0.39 g (1.2 mmol) of the product from stage 2, dissolved in 40 ml of THF, was added dropwise in the course of approximately 45 min. The mixture was stirred for a further 2 h at pH 12 (TLC checking), treated with 0.1 N HCI until a pH of 2 was achieved and extracted a number of times with ethyl acetate, and the extract was washed with water, dried and concentrated. The product was purified by chromatography on silica gel (dichloromethane/methanol 95:5). Yield: 0.393 g of melting point 216 °C Method D) Example 15 Tromethamine salt of 2-(4'-chlorobiphenyl-4-sulfonylamino)-5-(2,3-dihydroindol-1 yl)-5-oxopentanoic acid 104.5 g (0.21 mol) of the compound according to Example 8 were suspended in 1000 ml of ethanol and warmed to 5O°C. 25.4 g of tromethamine (2-amino-2-hydroxymethyl-1,3-propanediol, TRIS) dissolved in 100 ml of water were then added thereto. The mixture was briefly heated to reflux and filtered hot. The clear filtrate was allowed to cool to RTt whereupon the salt precipitated. It was filtered off with suction and washed twice with 150 ml of ethanol/water in a ratio of 9 to 1 each time and dried in a desiccator. Yield: 93.9 g (72%) of tromethamine salt Example 16 Tris-(2-hydroxyethyl)ammonium 2-(4'-chlorobiphenyl-4- sulfonylamino)-5-(2,3-dihydroindol-1-yl)-5-oxopentanoate 0.05 g of the product from Example 8 was dissolved in 3.18 g of acetone, 0.01 g of triethanolamine was added, and the solution was filtered and placed in an open Erlenmeyer vessel in a TLC chamber whose bottom was covered to approximately 1 cm with tert-butyl methyl ether. Crystal formation commenced after approximately 10 h and was complete after 4 to 5 days. The supernatant solvent was decanted off and the product obtained was dried under reduced pressure. Yield: 0.06 g of melting point 120°C. Examples 1 to 5 of Table 1 were prepared according to method A), Examples 6 and 7 according to method B) and Examples 8 to 14 according to method C). The starting material needed for the introduction of the arylsulfonyl side chain of Example 13 (4'-morpholin-4-ylbiphenyl-4-sulfonyl chloride) was prepared analogously to method C), stage 1. Pharmacological examples Preparation and determination of the enzymatic activity of the catalytic domain of human stromelysin and of neutrophil collagenase. The two enzymes - stromelysin (MMP-3) and neutrophil collagenase (MMP-8) - were prepared according to Ye et al. (Biochemistry; 31 (1992) pages 11231-11235) or Weithmann et al. (Inflamm Res 46(1997) 246-252). For measurement of the inhibitory action on the enzymatic activity, 70 µl of buffer solution and 10 µl of enzyme solution were incubated for 15 minutes with 10 µl of a 10% strength (v/v) aqueous dimethyl sulfoxide solution which optionally contained the enzyme inhibitor. After addition of 10 µl of a 10% strength (v/v) aqueous dimethyl sulfoxide solution which contained 1 mmol/l of the substrate, the enzyme reaction was monitored by fluorescence spectroscopy (328 nm (ex)/393 nm (em)). The enzyme activity was shown as the extinction increase/minute. The IC50 values listed in Table 2 were determined as those inhibitor concentrations which in each case led to a 50% inhibition of the enzyme. The buffer solution contained 0.05% of Brij (Sigma, Deisenhofen, Germany) and 0.1 mol/l of piperazine- N,N'-bis[2-ethane§ulfonic acid]/NaOH, 0.1 mol/l of NaCI, 0.01 mol/l of CaCI2 and 0.1 mol/I of piperazine-N,N'-bis[2-ethanesulfonic acid] (pH=6.5). The enzyme solution contained 5 µg/ml of one of the enzyme domains prepared according to Ye et al. The substrate solution contained 1 mmol/l of the fluorogenic substrate (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-3-(2',4'-dinitrophenyl)-L-2,3-diaminopropionyl-Ala-Arg-NH2 (Bachem, Heidelberg, Germany). Determination of the solubility in water of the free acid of 2-(4'-chloro-biphenyl-4-sulfonylamino)-5-(5-fiuoro-2,3-dihydroindol-1-yl)-5-oxo-pentanoic acid according to Example 8 and its tromethamine salt according to Example 15 1. Materials employed 1.1 Test substances a) Compound according to Example 8; 2-(4'-chlorobiphenyl-4-sulfonylamino)-5-(5-fluoro-2,3-dihydroindol-1-yl)-5-oxopentanoicacid b) Compound according to Example 15; tromethamine salt of 2-(4'-chlorobiphenyl-4-sulfonylamino)-5-(5-fluoro-2,3-dihydroindol-1-yl)-5-oxopentanoic acid 1.2 Reagents - Water: deionized, - Acetonitrile: LiChrosolv (Merck), - Diethylamine: for synthesis (Merck), - Acetic acid: concentrated and 1N (Merck) 2. Experimental procedure 2.1 2-(4^Chlorobiphenyl-4-sulfonylamino)-5-(5-fluoro-2,3-dihydroindol-l- yl)-5-oxopentanoic acid Approximately 50 mg of test substance according to 1.1a) were initially introduced into a 100 ml Erlenmeyer flask. After addition of 10 mi of water, the mixture was stirred at 25°C. After 2 hours (h) and after 3 ht aliquots were taken and centrifuged. 1 ml each of the supernatant was diluted to 10 ml with mobile phase. The concentration of the active component was determined by means of HPLC against an external standard. Repetition of the experiment. 2.2 Tromethamine salt of 2-(4'-chlorobiphenyl-4-sulfonylamino)-5-(5- fluoro-2,3-dihydroindol-1-yl)-5-oxopentanoic acid Approximately 800 mg of the tromethamine salt according to 1.1b) were initially introduced into a 100 ml Erlenmeyer flask. After addition of 10 ml of water, the mixture was stirred at 25°C. After 2 h and after 3 h, aliquots were taken and centrifuged. 1 ml each of the supernatant was diluted to 100 ml with mobile phase. The concentration of the active component was determined by means of HPLC against an external standard. Repetition of the experiment. 3. Analysis Instrument: Gynkotek HPLC unit Column: Stationary phase: ChiraDex 5 µm, Merck Dimensions: 250 mm x 4.0 mm Mobile phase: Acetonitrile 440 ml; water 560 ml; diethylamine 1 ml; adjusted to pH 6.4 using acetic acid. Injection volume: 10 µl Flow: 0.8 ml/min Column temperature: 4O°C Detection: UVat26l nm The time-dependent difference in the solubility values of 2-(4'-chloro-biphenyl-4-sulfonylamino)-5-(5-fluoro-2,3-dihydroindol-1-yl)-5-oxopentanoic acid could be attributable to supersaturation/saturation phenomena. As a result, the data value obtained after 3 h is used. The water solubility of the tromethamine salt of 2-(4'-chlorobiphenyl-4-sulfonylamino)-5-(5-fluoro-2,3-dihydroindol-1-yl)-5-oxopentanoic acid is higher approximately by a factor of 600 than that of the free acid of 2-(4f-chlorobiphenyl-4-sulfonylamino)-5-(5-fluoro-2,3-dihydroindol-1-yl)-5-oxopentanoic acid. WE CLAIM: 1. A compound of the formula 1 and/or a stereoisomeric form of the compound of the formula I and/or a physiologically tolerable salt of the compound of the formula I, where R1 is 1. phenyl, 2. phenyl, which is mono- or disubstituted by 2.1. (C‹i-C6)-alkyl, which is linear, cyclic or branched, 2.2. hydroxyl, 2.3. (Ci-C6)-alkyl-C(0)-0-, 2.4. (Ci-C6)-alkyl-0-, 2.5. (Ci-Ce)-alkyl-0-(Ci-C4)-alkyK)-, 2.6. halogen, 2.7. -CF3, 2.8. -CN, 2.9. -N02, 2.10. HO-C(O)-, 2.11. (Ci-C6)-alkyl-0-C(0)-, 2.12. methylenedioxo, 2.13. R4-(R5 )N-C(O)-, 2.14. R4-(R5)N-, or 2.15. the radical of a heterocycle from the following group: isoxazolidine, morpholine, isothiazolidine, thio-morpholine, pyrazolidine, imidazolidine, piperazine, azetidine, pyrrole, pyrroline, pyrrolidine, pyridine, azepine, piperidine, oxazole, isoxazole, imidazole, pyrazole, thiazole, isothiazole, diazepine, thiomorpholine, pyrimidine and pyrazine, which is unsubstituted or substituted as described for R1 under 2.1 to 2.14, or 3. a heteroaromatic from the following group 3.1. to 3.15., which is unsubstituted or substituted as described for R1 under 2.1 to 2.14, 3.i. pyrrole, 3.2. pyrazole, 3.3. imidazole, 3.4. triazole, 3.5. thiophene, 3.6. thiazole, 3.7. oxazole, 3.8. isoxazole, 3.9. pyridine, 3.10. pyrimidine, 3.11. indole, 3.12. benzothiophene, 3.13. benzimidazole, 3.14. benzoxazole or 3.15. benzothiazole, 2 4 5 R , R and R are identical or different and are 1. a hydrogen atom, 2. (Ci-C6)-alkyl-, 3. HO-C(OHCi-C6)-alkyl-, 4. phenyl-(CH2)n-, in which phenyl is unsubstituted or mono- or disubstituted as described for R1 under 2.1. to 2.14. and n is the integer zero, 1 or 2, or 5. picolyl or 6. R and R , together with the ring amino group, form a 4- to 7-membered ring in which, if appropriate, one of the carbon atoms is replaced by -0-, -S- or -NH-, R3is 1. -(Ci-C4)-alkyl-C(0)-N(R6)-R7. in which R6 and R7 together with the nitrogen to which they are bonded form a radical of the subformula Ha, lib or He where in the subformulae lla( lib and lie q is the integer zero, 1 or 2 and r is the integer zero or 1, Z is a carbon, nitrogen, oxygen or sulfur atom or a covalentbond, and R is a hydrogen atom or has the meaning described for R1 above under 2.1 to 2.14, 2. -(Ci-C4)-alkyl-C(0)-Y, in which Y is a radical of the subformula He or lid where in the subformulae He and lid o R is a hydrogen atom or has the meaning described for R above under 2.1 to 2.14 and R9 'S 2.1 a hydrogen atom 2.2 (Ci-Ce)-alkyh 2.3 HO-C(0)-(Ci-C6)-alkyh 2.4 'phenyl-(CH2)n-» in which phenyl is unsubstituted or mono- or disubstituted as described for R under 2.1 to 2.14 and n is the integer zero, 1 or 2, or 2.5 picolyl, or 3. -(Ci^C4)-alkyl-C(0)-N(R9HCH2)0-N(R4)-R5 , where g R has the abovementioned meaning, o is the integer 2, 3, 4 or 5 and 4 5 R and R together with the ring amino group form a 4- to 7-membered ring from the group consisting of isoxazolidine, morpholine, isothiazolidine, thiomorpholine, pyrazolidine, imidazolidine, piperazine, acetidine, Dvrroline. azeoine. oioeridine and diazeoine A is a) a covalent bond, b) -0-, c) -CH=CH- or d) -CsC-, B is a) -(CH2)m-. in which m is the integer zero, 1, 2, 3, 4, 5 or 6, b) -O-(CH2)p, in which p is an integer from 1 to 5, or c) -CH=CH-and X is -CH=CH-, an oxygen atom or a sulfur atom, with the exception of the compounds 2-(biphenyl-4-sulfonylamino)-5-(2,3-dihyroindol-1-yl)-5-oxo-pentanecarboxylicacid and 5-(2,3-dihydroindol-1-yl)-5-oxo-2-(4'-pyrrolidin-1-ylbiphenyl-4-sulfonylamino)pentanoic acid and with the exception of the case in which the subformula lib r is the integer zero and z is a covalent bond. 2. The compound of the formula I as claimed in claim 1, wherein R is 1. phenyl or 2. phenyl, which is monosubstituted by 2.1. (C1-C6)-alkyl-, in which alkyl is linear, cyclic or branched, 2.2. -OH, 2.3. (Ci-C6)-alkyl-C(O)-O-, 2.4. (C1-C6)-alkyl-O-, 2.5. (C1-C6)-alkyl-O-(C1-C4)-alkyl-0-, 2.6. halogen, 2.7. -CF3 or 2.8. R4-(R5 )N-, 2 4 5 R , R and R are identical or different and are 1. a hydrogen atom, 2. (Ci-C6)-alkyl-or 4 5 3. R and R together with the ring amino group form a 4- to 7-membered ring, in which, if appropriate, one of the carbon atoms is replaced by -0-, -S- or -NH-, R3is1. -(Ci-C4)-alkyl-C(0)-N(R6)-R7. in which R6 and R7 together with the nitrogen to which they are bonded form a radical of the subformula lla, where in the subformula lla q is the integer zero or 1, Z is a carbon, nitrogen, oxygen or sulfur atom, and g R is a hydrogen atom or has the meaning described for R above under 2.1 to 2.8, 2. -(Ci-C4)-alkyl-C(0)-Y, in which Y is a radical of the subformula lie or lid where in the subformulae lie and lid 8 R is a hydrogen atom or has the meaning described for R above under 2.1 to 2.8 and g R is a hydrogen atom, or 3. -(Ci-C4)-aIkyl-C(0)-N(R9).(CH2)o-N(R4)-R5 , where g R is a hydrogen atom, o is the integer 2 or 3 and 4 5 R and R are identical or different and are 1. a hydrogen atom, 2. (C‹i-C6)-alkyl-or 4 5 3. R and R together with the ring amino group form a 4- to 7-membered ring from the group consisting of isoxazolidine, morpholine, isothiazolidine, thiomorpholine, pyrazolidine, imidazolidine, piperazine, acetidine, pyrroline, pyrrolidine, azepine, piperidine and diazepine, A is a covalent bond or -0-, B is a) -(CH2)m-, in which m is the integer zero, 1 or 2, or b) -O-(CH2)p, in which p is the integer 1 or 2, and X is -CH=CH-. 3. The compound of the formula I as claimed in claim 1, wherein 1 R is 1. phenyl or 2. phenyl, which is monosubstituted by 2.1. chlorine, 2.2. bromine, 2.3. fluorine, 2.4. pyrrolidine or 2.5. morpholine, R is a hydrogen atom, R3is 1. -(Ci-C4)-alky\|.C(0)-N(R6)-R7, in which R6 and R7 together with the nitrogen to which they are bonded form a radical of the subformula Ma, where in the subformula fla q is the integer zero or 1, Z is a carbon atom, and Q R is a hydrogen atom, chlorine, bromine or fluorine, 2. -(Ci-C4)-alkyl-C(0)-Y, in which Y is a radical of the subformula He or lid where in the subformulae lie and lid 8 9 R and R are each a hydrogen atom, or 3. -(Ci-C4)-alkyl-C(0)-N(R9HCH2)o-N(R4)-R5 , where g R is a hydrogen atom, o is the integer 2 or 3 and 4 5 R and R are identical or different and independently of one another are 1. a hydrogen atom, 2. phenyl or 3. morpholine, A is a covalent bond or -0-f B is a covalent bond and X is -CH=CH-. The compound of the formula l as claimed in claim 1 or 2, which is a compound from the group consisting of 2-(biphenyl-4- sulfonylamino)-4-(naphthalen-1-ylcarbamoyl)butyric acid, 2-(biphenyl-4-sulfonylamino)-4-(naphthalen-2-yl-carbamoyl)butyric acid, 2-(biphenyl-4-sulfonylamino)-4-(2-phenylaminoethyl- carbamoyl)butyric acid, 2-(4'-chlorobiphenyl-4-sulfonylamino)-4-(3-morpholin-4-yl-propylcarbamoyl)butyric acid, 4«(3-(4-(biphenyl-4-sulfonylamino)-4-carboxybutyrylamino)propyl)morpholin-4Hum trifluoroacetate, 2-(4•- chlorobiphenyl-4-sulfonylamino)-5-(2,3-dihydroindol-1-yl)-5-oxopentanoic acid, 2-(4•-bromobiphenyl-4-sulfonylamino)-5- (2,3- dihydroindoM-yl)-5-oxopentanoic acid, 2-(4'-chloro-biphenyl-4- sulfonylamino)-5-(5-fluoro-2,3-dihydroindol-1- yl)-5-oxopentanoic acid, 2-(4•-bromobiphenyl-4-sulfonylamino)-5-(5~fluoro-2,3-dihydro- indol-1-yl)-5-oxopentanoic acid, 5-(5-fluoro-2,3-dihydroindol-1 -yl)-5- oxo-2-(4'-pyrrolidin-l -yl-biphenyl-4-sulfonylamino)pentanoic acid and 5-(5-fluoro-2,3-dihydroindol-1-yl)-2-(4'-morpholin-4-yl-biphenyl-4"Sulfonylamino)-5-oxopentanoicacid. The compound of the formula I as claimed in claim 4, which is a 2-amino-2-hydroxymethyl-1,3-propanediol salt, trimethylamine salt, triethylamine salt, ethanolamine salt or triethanolamine salt. 6. A process for the preparation of the compound of the formula I, as claimed in one or more of claims 1 to 5, which comprises a) reacting an aminocarboxylic acid of the formula II in which R is a hydrogen atom or an ester protective group which is customary in peptide chemistry, s is the integer zero, 1, 2 or 3, R10 is the radical -OR 2, in which R12 is an ester protective group, which can be cleaved in the presence of R , or a hydrogen atom, or R is a radical -N(R )-R , in 6 7 which R and R are as defined in formula I, with a sulfonic acid derivative of the formula III where R ,X,A and B are as defined in formula I and Y is a 13 13 halogen atom, imidazolyl or OR , in which R is a hydrogen atom, (C-i-C6)-alkyl, phenyl or benzyl, which is optionally substituted, in the presence of a base or, if appropriate, of a dehydrating agent to give a compound of the formula IV in which R1, A, X, B, R2, s, R10 and R11 have the abovementioned meaning, and then removing a protective group R possibly included in the radical R10 in the presence of the protective group R , and then introducing the radical ß 7 -N(R )-R by appropriate activation, known from peptide chemistry, of the carboxyl group, and subsequently removing the included protective group R and obtaining the compound of the formula l, or b) reacting an amino acid anhydride of the formula V 13 14 in which R is a hydrogen atom and R is an N-protective 13 14 group known from peptide chemistry, or R and R are a cyclic N-protective group, e.g. phthaiimido and s is as defined above under process a), with a primary or secondary radical -N(R )-R in the sense of a ring opening to give an intermediate of the formula VI in which R , R , s, R and R have the abovementioned meaning, where this ring opening, depending on the protective group and reaction conditions as a rule regioselectively yields the isomer shown in formula VI, which, if regioisomer mixtures occur, can be further enriched by crystallization or chromatography, then cleaving the included protective group R and/or R with release of the amine, and then carrying out, as described under a), an N-sulfonation with a sulfonic acid derivative of the formula III which leads to a product of the formula I, or c) separating a compound of the formula I prepared according to process a) or b)t which on account of its chemical structure occurs in enantiomeric forms, into the pure enantiomers by salt formation with enantiomerically pure acids or bases, chromatography on chiral stationary phases or derivatization by means of chiral enantiomerically pure compounds such as amino acids, separation of the diastereomers thus obtained, and removal of the chiral auxiliary groups, or d) isolating the compound of the formula I prepared according to process a), b) or c) in free form or, if acidic or basic groups are present, converting it into physiologically tolerable salts. 7. A pharmaceutical, which contains an efficacious amount of a compound of the formula I as claimed in one or more of claims 1 to 5 together with a pharmaceutically suitable and physiologically tolerable excipient, additive and/or other active compounds and auxiliaries. 8. A process for the production of a pharmaceutical, which comprises bringing at least one compound of the formula I as claimed in one or more of claims 1 to 5 into a suitable administration form using a pharmaceutically suitable and physiologically tolerable vehicle and, if appropriate, further suitable active compounds, additives or auxiliaries.

Full Text

Description
N-Arylsulfonylamino acid omega-amides
The invention relates to novel N-aryisulfonylamino acid omega-amides, processes for their preparation, and use thereof as pharmaceuticals.
The applications EP 0 606 046, WO 95/35276 and WO 96/27583 describe arylsulfonaminohydroxamic acids and their action as matrix metalloproteinase inhibitors. Specific arylsulfonamidocarboxylic acids are used as intermediates for the preparation of thrombin inhibitors (EP 0 468 231) and aldose reductase inhibitors (EP 0 305 947). The application EP 0 757 037 also describes the action of sulfonylamino acid derivatives as metalloproteinase inhibitors.
In the effort to find efficacious compounds for the treatment of connective tissue disorders, it has now been found that the sulfonylaminocarboxylic acids according to the invention are strong inhibitors of matrix metalloproteinases. Particular value is placed here on the inhibition of stromelysin (MMP-3) and neutrophii collagenase (MMP-8), as both enzymes are decisively involved in the degradation of the proteoglycans, as important constituents of the cartilaginous tissue (A. J. Fosang et al. J. Clin. Invest. 98 (1996) 2292-2299). Those enzymes which are involved in the constitutive degradation and synthesis of matrix constituents likewise belong to the protein family of the matrix metalloproteinases. For example, MMP-1 (collagenase -1) has an important vital function, since it is involved in natural collagen degradation, in particular even where morphogenetic changes take place. Medicinal active compounds which, although they are able to inhibit MMP-3 and MMP-8, at the same time leave MMP-1 largely unaffected, are thus preferred. Such an active compound can even be particularly preferred with respect to the healing of the human or animal body, which, with, all in all, only moderate inhibition of MMP-3 and -8, shows no or a weaker effect on the MMP-1.

and/or a stereoisomeric form of the compound of the formula I and/or a physiologically tolerable salt of the compound of the formula I, where R is 1. phenyl,
2. phenyl, which is mono- or disubstituted by
2.1. (C-i-C6)-alkyl, which is linear, cyclic or branched,
2.2. hydroxyl,
2.3. (Ci-C6)-alkyl-C(0)-0-,
2.4. (Ci-C6)-alkyl-O-,
2.5. (Ci-Ce)-alkyl-0-(Ci-C4)-alkyl-0,
2.6. halogen,
2.7. -CF3,
2.8. -CN,
2.9. -N02,
2.10. HO-C(O)-,
2.11. (Ci-C6)-alkyl-0-C(0)-,
2.12. methylenedioxo,
2.13. R4-(R5 )N-C(O)-,
2.14. R4-(R5)N-,or
2.15. a heterocycle from the following group: isoxazolidine,
morpholine, isothiazolidine, thiomorpholine,
pyrazolidine, imidazolidine, piperazine, azetidine,
pyrrole, pyrroline, pyrrolidine, pyridine, azepine,
piperidine, oxazole, isoxazole, imidazole, pyrazole,
thiazole, isothiazole, diazepine, thiomorpholine,
pyrimidine and pyrazine, which is unsubstituted or
substituted as described under 2.1 to 2.14, or
3. a heteroaromatic from the following group 3.1. to 3.15., which
is unsubstituted or substituted as described under 2.1 to 2.14,
3.1. pyrrole,
3.2. pyrazole,
3.3. imidazole,
3.4. triazole,
3.5. thiophene,
3.6. thiazole,
3.7. oxazole,
3.8. isoxazole,
3.9. pyridine,
3.10. pyrimidine,
3.11. indole,

3.12. benzothiophene,
3.13. benzimidazole,
3.14. benzoxazole or
3.15. benzothiazole,
2 4 5
R , R and R are identical or different and are
1. a hydrogen atom,
2. (Ci-Ce)-alkyh
3. HO-C(OMCi-C6)-alkyl-,
4. phenyl-(CH2)m in which phenyl is unsubstituted or mono- or
disubstituted as described under 2.1. to 2.14. and n is the
integer zero, 1 or 2, or
5. picolyl or
6. R and R , together with the ring amino group, form a 4- to
7-membered ring in which, if appropriate, one of the carbon
atoms is replaced by -0-, -S- or -NH-,
R3is1. -(Ci-C4)-alkyl-C(0)-N(R6)-R7, in which R6 and R7 together
with the nitrogen to which they are bonded form a radical of the subformula lla, Mb or lie

where in the subformulae lla, lib and lie q is the integer zero, 1 or 2 and r is the integer zero or 1,
Z is a carbon, nitrogen, oxygen or sulfur atom or a covalent bond, and
Q
R is a hydrogen atom or has the meaning described for R1 above under 2.1 to 2.14, 2. -(Ci-C4)-alkyl-C(O)-Y,

in which Y is a radical of the subformula lie or lid

where in the subformulae lie and lid
o
R is a hydrogen atom or has the meaning described for R above under 2.1 to 2.14 and
g
R is 2.1 a hydrogen atom
2.2 (Ci-C6)-alkyl-,
2.3 HO-C(0)-(Ci-C6)-alkyl-,
2.4 phenyl-(CH2)rr, in which phenyl is
unsubstituted or mono- or disubstituted
1
as described for R under 2.1 to 2.14 and n is the integer zero, 1 or 2, or
2.5 picolyl, or
3. •(Ci-C4)-alkyl-C(O)-N(R^-(CH2)o-N(R4)^5 , where g R has the abovementioned meaning,
0 is the integer 2, 3, 4 or 5 and
4 5
R and R have the abovementioned meaning, A is a) a covalent bond,
b) -0-,
c) -CH=CH- or
d) -OC-,
B is a) -(CH2)m-1 in which m is the integer zero, 1,2,3,4,5 or 6,
b) -O-(CH2)p, in which p is an integer from 1 to 5, or
c) -CH=CH- and
X is -CH=CH-, an oxygen atom or a sulfur atom.
A compound of the formula I is preferred, where R is 1. phenyl or
2. phenyl, which is monosubstituted by
2.1. (Ci-Ce)-alkyl-, in which alkyl is linear, cyclic or
branched,
2.2. -OH,

2.3. . (Ci-Ce)-alkyl-C(0)-0-,
2.4. (Ci-C6)-alkyl-0-,
2.5. (C1-C6)-alkyl-0-(C1-C4)-alkyl-O,
2.6. halogen,
2.7. -CF3 or
2.8. R4-(R5 )N-, 2 4 5
R , R and R are identical or different and are
1. a hydrogen atom,
2. (d-C6)-alkyl-or
4 5
3. R and R together with the ring amino group form a 4- to
7-membered ring, in which, if appropriate, one of the carbon
atoms is replaced by -0-, -S- or -NH-,
R3is 1. -(Ci-C4)-alkyl-C(0)-N(R6)-R7i in which R6 and R7 together
with the nitrogen to which they are bonded form a radical of
the subformula Ha, where in the subformula lla
q is the integer zero or 1,
Z is a carbon, nitrogen, oxygen or sulfur atom, and
Q
R is a hydrogen atom or has the meaning described for 1 R above under 2.1 to 2.8,
2, -(Ci-C4)-alkyt-C(0)-Y, in which
Y is a radical of the subformula lie or lid where in the subformulae lie and lid
o
R is a hydrogen atom or has the meaning described for R above under 2.1 to 2.8 and
g
R is a hydrogen atom, or
3. -(Ci-C4)-alkyl-C(0).N(R9)-(CH2)0-N(R4)-R5 , where
g
R is a hydrogen atom, o is the integer 2 or 3 and
4 5
R and R are identical or different and are
1. a hydrogen atom,
2. (C1-C6)-alkyl-or
4 5
3. R and R together with the ring amino group
form a 4- to 7-membered ring, in which, if
appropriate, one of the carbon atoms is
replaced by -0-, -S- or -NH-,.
A is a covalent bond or -0-,
B is a) -(CH2)m-. in which m is the integer zero, 1 or 2, or b) -O-(CH2)p, in which p is the integer 1 or 2, and

X is -CH=CH-.
A compound of the formula I is furthermore preferred, where R is 1. phenyl or
2. phenyl, which is monosubstituted by
2.1. chlorine,
2.2. bromine,
2.3. fluorine,
2.4. pyrrolidine or
2.5. morpholine, R is a hydrogen atom,
R3is 1. -(Ci-C4)-alky|.C(0)-N(R6)-R7» in which R6 and R7
together with the nitrogen to which they are bonded form a radical of the subformula I la, where in the subformula Ha
q is the integer zero or 1, Z is a carbon atom, and
o
R is a hydrogen atom, chlorine, bromine or fluorine,
2. -(Ci-C4)-alkyl-C(O)-Y, in which
Y is a radical of the subformula lie or lid where in the subformulae lie and lid
8 9
R and R are each a hydrogen atom, or
3. -(Ci-C4)"alkyl-C(0)-N(R9HCH2)o-N(R4)-R5, where
g
R is a hydrogen atom,
o is the integer 2 or 3 and
4 5
R and R are identical or different and independently of one another are
1. a hydrogen atom,
2. phenyl or
3. morpholine, A is a covalent bond or -0-,
B is a covalent bond and X is -CH=CH-.
The compounds 2-(biphenyl-4-sulfonylamino)-4-(naphthalen-1-ylcarb-amoyl)butyric acid, 2-(biphenyl-4-sulfonylamino)-4-(naphthalen-2-yl-carbamoyl)butyric acid, 2-(biphenyl-4-sulfonylamino)-4-(2-phenylamino-

ethylcarbamoyl)butyric acid, 2-(4'-chloro-biphenyl-4-sulfonylamino)-4-(3-morpholin-4-ylpropylcarbamoyl)butyric acid, 4-(3-(4-(biphenyl-4-sulfonyl-amino)-4-carboxybutyrylamino)propyl)morpholin-4-ium trifluoroacetate, 2-(biphenyl-4-sulfonylamino)-5-(2,3-dihydroindol-1-yl)-5-oxopentanoic acid, 5-(2,3-dihydroindol-1-yl)-5-oxo-2-(4'-pyrroiidin-1-yl-biphenyl-4-sulfonyl-amino)pentanoic acid, 2-(4'-chlorobiphenyl-4-sulfonylamino)-5-(2,3-dihydroindol-1-yl)-5-oxopentanoic acid, 2-(4'-bromobiphenyl-4-sulfonyl-amino)-5-(2,3-dihydroindoM-yl)-5-oxopentanoic acid, 2-(4'-chloro-biphenyl-4-sulfonylamino)-5-(5-fluoro-2,3-dihydroindol-1-yl)-5-oxopentanoic acid, 2-(4'-bromobiphenyl-4-suifonylamino)-5-(5-fluoro-2,3-dihydroindoi-1-yl)-5-oxopentanoic acid, 5-(5-fluoro-2,3-dihydroindol-1 -yl)-5-oxo-2-(4'-pyrrolidin-1-ylbiphenyl-4-sulfonylamino)pentanoic acid or 5-(5-fiuoro-2,3-dihydroindol-l-yl)-2-(4'-morpholin^-yl-biphenyM-suifonylamino)-5•oxopentanoic acid are particularly preferred.
4 5
The expression " R and R together with the ring amino group form a 4- to 7-membered ring and/or one of the carbon atoms is replaced by -0-, -S- or -NH-" is understood as meaning radicals which are derived, for example, from isoxazolidine, morpholine, isothiazolidine, thiomorpholine, pyrazolidine, imidazolidine, piperazine, azetidine, pyrrole, pyrroline, pyrrolidine, pyridine, azepine, piperidine, pyrazole or diazepine. The term "halogen" is understood as meaning fluorine, chlorine, bromine or iodine. The term "alky!" is understood as meaning hydrocarbon radicals whose carbon chain is linear or branched.
The starting substances of the chemical reactions are known or can easily be prepared by methods known from the literature.
The invention furthermore relates to a process for the preparation of the compound of the formula I and/or of a stereoisomeric form of the compound of the formula I and/or of a physiologically tolerable salt of the compound of the formula I, which comprises (as described in method A, see examples)

11 in which R# is a hydrogen atom or an ester protective group which
is customary in peptide chemistry, s is the integer zero, 1, 2 or 3,
R is the radical -OR , in which R is an ester protective group,
11 which can be cleaved in the presence of R , or a hydrogen atom, or
R is a radical -N(R )-R , in which R and R are as defined in
formula I,
with a sulfonic acid derivative of the formula III

where R ,X,A and B are as defined in formula I and Y is a halogen
13 13
atom, imidazolyl or OR , in which R is a hydrogen atom, (C‹i-C6)-alkyl, phenyl or benzyl, which is optionally substituted, in the presence of a base or, if appropriate, of a dehydrating agent to give a compound of the formula IV

in which R , A, X, B, R , s, R and R have the abovementioned
12 meaning, and then removing a protective group R possibly
included in the radical R10 in the presence of the protective group R , and then introducing the radical -N(R )-R by appropriate
activation, known from peptide chemistry, of the carboxyl group, and
11 removing the included protective group R and obtaining the
compound of the formula I, or (as described in methods B and C, see examples) i) reacting an amino acid anhydride of the formula V

13 14
in which R is a hydrogen atom and R is an N-protective group
known from peptide chemistry, such as, for example, carbobenzyloxy (Z), or R and R are a cyclic N-protective group, e.g. phthalimido, and s is as defined above,
with a primary or secondary radical -N(R )-R in the sense of a ring opening to give an intermediate of the formula VI

in which R , R , s, R and R have the abovementioned meaning, where this ring opening, depending on the protective group and reaction conditions (cf. X. Huang, X. Luo, Y. Roupioz, J. W. Keillor, J. Org. Chem. 1997, 62, 8821-8825) as a rule regioselectively yields the isomer shown in formula VI, which, if regioisomer mixtures occur, can be further enriched by crystallization or chromatography, then cleaving the included protective group R and/or R with release
of the amine, and then carrying out, as described under a) an N-sulfonation with a sulfonic acid derivative of the formula III which leads to a product of the formula I, or c) resolving a compound of the formula I prepared according to process a) or b), which on account of its chemical structure occurs in enantiomeric forms, into the pure enantiomers by salt formation with enantiomerically pure acids or bases, chromatography on chiral stationary phases or derivatization by means of chiral enantiomerically pure compounds such as amino acids, separation of the diastereomers thus obtained, and removal of the chiral auxiliary groups, or

d) isolating the compound of the formula I prepared according to process a), b) or c) in free form or, if acidic or basic groups are present, converting it into physiologically tolerable salts.
Suitable protective groups for this are preferably the customary N-protective groups used in peptide chemistry, for example protective groups of the urethane type, benzyloxycarbonyl (Z), t-butyloxycarbonyl (Boc), 9-fluorenyloxycarbonyl (Fmoc), allyloxycarbonyl (Alloc) or of the acid amide type, in particular formyl, acetyl or trifluoroacetyl, and of the alkyl type, for example benzyl.
Starting materials used for the preparation of the sulfonic acid derivatives of the formula III are preferably sulfonic acids or their salts of the formula VII, for example

15 1
where R is a radical described for R under 2.1. to 2.14.
15
Sulfonic acid derivatives of the formula III, which like R contain a
4 5
secondary or cyclic amine of the type -N(R )-R , are prepared preferably and in high yields by Pd-catalyzed substitution of a bisaryl halide, preferably of a bromide, by a secondary amine following known literature procedures (cf. A.S. Guram, R.A. Rennels, S.L Buchwald; Angew. Chem. 1995, 107, 1456-1459) and subsequent sulfochlorination by means of chlorosulfonic acid. The sulfochloride function is in this case preferably guided into the desired para position by the directing effect of the amine group.
The catalyst dichlorobis(tritolylphosphine)palladium(ll) advantageously used can be prepared analogously to R.F. Heck in "Palladium Reagents in Organic Syntheses", Academic Press, London (1985), p. 18 starting from tri-o-tolylphosphine, palladium(ll) chloride and LiCI in methanol.
For the preparation of the arylsulfonic acids of the formulae Vila and Vllb, the sulfonation process with concentrated sulfuric acid described in Houben-Weyl "Methoden der Organischen Chemie" [Methods of Organic Chemistry] volume 9, pp. 540-546 is preferably used, if appropriate in the presence of a catalyst, sulfur trioxide and its addition compounds or halosulfonic acids, such as chlorosulfonic acid. Particularly in the case of the diphenyl ethers of the formula Vllb, the use of concentrated sulfuric acid and acetic anhydride as a solvent (cf. CM. Suter, J. Am. Chem. Soc. 53 (1931) 1114), or the reaction with excess chlorosulfonic acid (J.P. Bassin, R. Cremlyn and F. Swinbourne; Phosphorus, Sulfur and Silicon 72 (1992) 157) has proven suitable. Sulfonic acids according to the formulae Vile, Vlld or Vile can be prepared in a manner known per se by reacting the appropriate arylalkyl halide with sulfites such as sodium sulfite or ammonium sulfite in aqueous or aqueous/alcoholic solution, it being possible to accelerate the reaction in the presence of tetraorganoammonium salts such as tetrabutylammonium chloride.
Sulfonic acid derivatives according to formula III used, are, in particular, the suifonyl chlorides. For their preparation, the corresponding sulfonic acids,

also in the form of their salts such as sodium, ammonium or pyridinium salts, are reacted in a known manner with phosphorus pentachloride or thionyl chloride without or in the presence of a solvent such as phosphorus oxychloride or of an inert solvent such as methylene chloride, cyclohexane or chloroform, in general at reaction temperatures of 2O°C up to the boiling point of the reaction medium used. Advantageously, the direct sulfochlorination of the appropriate aromatic can also be carried out using chlorosulfonic acid, sulfuryl chloride or pyrosulfuryl chloride (Houben-Weyl, Methoden der Organischen Chemie [Methods of Organic Chemistry], volume 9 (1995), pages 572 - 579).
The reaction of the sulfonic acid derivatives of the formula III with the amino acids of the formula II according to process variants a) or b) proceeds advantageously in the style of the Schotten-Baumann reaction. Suitable bases for this are particularly alkali metal hydroxides such as sodium hydroxide, but also alkali metal acetates, alkali metal hydrogencarbonates, alkali metal carbonates and amines. The reaction takes place in water or in a water-miscible or nonmiscible solvent such as tetrahydrofuran (THF), acetone, dioxane or acetonitrile, the reaction temperature in general being kept at -10°C to 5O°C. In the case in which the reaction is carried out in anhydrous medium, tetrahydrofuran or methylene chloride, acetonitrile or dioxane in the presence of a base, such as triethylamine, N-methylmorpholine, N-ethyl- or diisopropylethylamine is especially used, optionally in the presence of N,N-dimethylaminopyridine as a catalyst.
In another variant, particularly when using polar starting materials which are present in unprotected form, the aminocarboxylic acids of the formula II can first be converted into their silylated form with the aid of a silylating agent such as bis-trimethylsilyl trifluoroacetamide (BSTFA) and they then react with sulfonic acid derivatives to give compounds of the formula IV.
The physiologically tolerable salts of the compounds of the formula I capable of salt formation, including their stereoisomeric forms, are prepared in a manner known per se. With basic reagents such as hydroxides, carbonates, hydrogencarbonates, alkoxides and ammonia or organic bases, for example 2-amino-2-hydroxymethyl-1,3-propanediol (tromethamine), trimethyl- or triethylamine, ethanolamine or triethanolamine or alternatively basic amino acids, for example lysine, ornithine or arginine, the carboxylic acids form stable alkali metal, alkaline earth metal or, if

appropriate, substituted ammonium salts. Salts of the compound of the formula I which are formed with the organic bases mentioned show a high water solubility. If the compounds of the formula I have basic groups, stable acid addition salts can also be prepared using strong acids. For this, both inorganic and organic acids such as hydrochloric, hydrobromic, sulfuric, phosphoric, methanesulfonic, benzenesulfonic, p-toluenesulfonic, 4-bromobenzenesulfonic, acetic, oxalic, tartaric, trifluoromethylsulfonic, cyclohexylamidosulfonic, succinic or trifluoroacetic acid are suitable.
The invention also relates to pharmaceuticals which contain an efficacious amount of at least one compound of the formula I and/or of a physiologically tolerable salt of the compound of the formula I and/or an optionally stereoisomeric form of the compound of the formula I, together with a pharmaceutically suitable and physiologically tolerable excipient, additive and/or other active compounds and auxiliaries.
On account of the pharmacological properties, the compounds according to the invention are suitable for the prophylaxis and therapy of all those disorders in whose course matrix-degrading metalloproteinases are involved. These include degenerative joint disorders such as osteoarthroses, rheumatoid arthritis, spondyloses, chondrolysis after joint trauma or relatively long immobilization of the joint, e.g. after meniscus or patella injuries or tearing of the ligaments. In addition, these also include disorders of the connective tissue such as collagenoses, periodontal disorders, which can even lead to the loss of the teeth, wound healing disorders and chronic disorders of the locomotory apparatus such as inflammatory, immunologically or metabolically caused acute and chronic arthritis, arthropathies, myalgias and disorders of the bone metabolism (such as osteoporosis). The medicinal use of the compounds of the formula I according to the invention may be indicated in the case of vascular diseases, e.g. blood vessel occlusions, atherosclerotic plaques or aneurysms, particularly in threatening rupture, or in stenoses of any pathogenesis. The compounds of the formula I are furthermore suitable for the treatment of inflammations, including wounds and ulcers, in particular also those of the skin, carcinomatous disorders, in particular also for blocking or checking the formation and spread of metastases, and also in carcinoma of the breast. The treatment of cachexia, anorexia, septic shock, periodontosis and periodontitis are further medicinal areas of application for the compounds according to the invention.

In general, the pharmaceuticals according to the invention are administered orally or parenterally. Rectal or transdermal administration is also possible.
The invention also relates to a process for the production of a pharmaceutical, which comprises bringing at least one compound of the formula I into a suitable administration form using a pharmaceutically suitable and physiologically tolerable vehicle, and, if appropriate, further suitable active compounds, additives or auxiliaries.
Suitable solid or pharmaceutical preparation forms are, for example, granules, powders, coated tablets, tablets, (micro)capsules, suppositories, syrups, juices, suspensions, emulsions, drops or injectable solutions as well as preparations with protracted release of active compound, in whose preparation customary auxiliaries, such as excipients, disintegrants, binders, coating agents, swelling agents, glidants or lubricants, flavorings, sweeteners and solubilizers are used. Frequently used auxiliaries which may be mentioned are magnesium carbonate, titanium dioxide, lactose, mannitol and other sugars, talc, lactoprotein, gelatin, starch, cellulose and its derivatives, animal and vegetable oils such as cod-liver oil, sunflower oil, peanut or sesame oil, polyethylene glycol and solvents such as, for example, sterile water and mono- or polyhydric alcohols such as glycerol.
The pharmaceutical preparations are preferably prepared and administered in dose units, each unit containing as active constituent a specific dose of the compound of the formula I according to the invention. In the case of solid dose units such as tablets, capsules, coated tablets or suppositories, this dose can be up to approximately 1000 mg, but preferably approximately 50 to 300 mg and, in the case of injection solutions in ampoule form, up to approximately 300 mg, but preferably approximately 10 to 100 mg.
For the treatment of an adult patient weighing approximately 70 kg, depending on the efficacy of the compounds according to formula I, daily doses of approximately 20 mg to 1000 mg of active compound, preferably approximately 100 mg to 500 mg, are indicated. Under certain circumstances, however, even higher or lower daily doses may be appropriate. The daily dose can be administered both by single administration in the form of an individual dose unit or else of several

smaller dose units and by multiple administration of subdivided doses at intervals.
The compounds on which the invention is based were identified by nuclear magnetic resonance and mass spectroscopy, where in the case of the presence of regio- or diastereoisomeric forms in addition to H also C and multidimensional NMR methods were employed for clear proof of structure. H NMR spectra have been recorded on a 400 MHz apparatus from Bruker, as a rule using tetramethylsilane (TMS) as an internal standard and at room temperature (RT). As a rule, final products are determined by mass spectroscopic methods (FAB-, ESI-MS with positive or negative ionization). Temperatures are in degrees Celsius, RT means room temperature (22°C to 26°C). Abbreviations used are either explained or correspond to the customary conventions.
Preparation examples
Method A)
Example 5 4-(3-(4-(Biphenyl-4-sulfonylamino)-4-carboxybutyrylamino)-
propyl)morpholin-4-iumtrifluoroacetate Stage 1: Sulfonation of glutamyl tert-butyl ester 3 g (0.0148 mol) of L-Glu-OtBu were dissolved in 29.5 ml of 0.5 N NaOH and 250 ml of tetrahydrofuran (THF) and cooled to 0°C in an ice bath, and simultaneously (while keeping the pH constant at pH 8.5), a solution of 4.476 g (0.0177 mol) of biphenylsulfonyl chloride in 35 ml of THF and 29.52 ml of an aqueous 0.5 N NaOH was slowly added dropwise over approximately 30 minutes (min). After removing the ice bath, the mixture was stirred at room temperature for 3 hours (h) and the completion of the reaction was monitored by thin-layer chromatography (TLC). THF was removed under reduced pressure, the residue was extracted once with diethyl ether, the extract was acidified to pH 1.5 using 1 N HCI, the product was extracted a number of times with ethyl acetate, the extract was dried and the solvent was removed under reduced pressure. 2.89 g of product remained, which could be reacted further without further purification. Stage 2: Conversion into the amide
0.25 g (0.596 mmol) of the product from stage 1 was dissolved in 50 ml of absolute THF, 0.119 g (0.63 mmol) of EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride) and 0.0845 g (0.55 mmol) of 1-hydroxy-benzotriazole hydrate were added, the mixture was stirred for 30 min, then 0.268 ml (1.79 mmol) of N-(3-aminopropyl)morpholine was added

carefully, and the mixture was stirred for approximately 8 h. It was taken up
with water, acidified using 1N HCI to pH 2, extracted a number of times with
ethyl acetate, washed with dilute NaCI, dried and concentrated. Yield: 250
mg.
A further purification could be carried out by chromatography on silica gel
by means of dichloromethane/methanol 9:1.
Stage 3: Removal of the protective group
0.11 g of the product from stage 2 was dissolved in 5 ml of trifluoroacetic
acid and 5 ml of dichloromethane and the mixture was stirred at room
temperature for approximately 3 h under protective gas until completion of
the reaction (TLC checking). After concentration, the mixture was entrained
a number of times with dichloromethane and the residue was dried under
reduced pressure. 0.11 g of the compound of Example 5 remained as the
trifluoroacetate.
Method B)
Examples 2-(Biphenyl-4-sulfonylamino)-5-(2,3-dihydroindol-1-yl)-5-oxo-
pentanecarboxylic acid Stage 1: Reaction of phthaloyl-L-glutamyl anhydride with dihydroindole 1.5 g (0.0058 mol) of phthaloyl-L-glutamyl anhydride were dissolved in 15 ml absolute dioxane, and a solution of 0.813 ml (0.0073 mol) of dihydroindole in 15 ml of dioxane was added dropwise in the course of 10min. The mixture was heated until the reaction was complete (approximately 5 h, TLC checking) at 40 °C, concentrated, and the mixture was entrained a number of times under reduced pressure using dichloromethane. Yield: 2.7 g (product contained approximately 15 mol% of dioxane)
Stage 2: Removal of the protective group
2.43 g of the product from stage 1 were dissolved in 30 ml of ethanol and 0.39 ml (0.0080 mol) of hydrazine hydrate was added. The mixture was stirred at 8O°C for 3 h, concentrated to dryness under reduced pressure, the residue was taken up using 50 ml of 25% strength aqueous acetic acid and the mixture was heated at 8O°C for approximately 10 min. It was then cooled in an ice bath and the resulting precipitate was filtered off with suction, the precipitate was taken up again using acetic acid and this procedure was repeated. The phthalyl hydrazide residue which remained was discarded. The combined filtrates were concentrated, and afforded 0.7 g of product.

Stage 3: Introduction of the N-arylsulfonyl radical 0.5 g (0.002 mol) of the product from stage 2 was dissolved in 50 ml of THF/water (1:1) together with 0.45 g (0.0033 mol) of potassium carbonate, and a solution of 0.61 g (0.0024 mol) of biphenylsulfonyl chloride, dissolved in 50 ml of THF, was added dropwise in the course of 20 min. The mixture was stirred at RT until the reaction was complete (approximately 6 to 8 h, TLC checking), extracted once with diethyl ether and acidified to pH 1.5 using 1N HCI, and the product was extracted a number of times with ethyl acetate, dried and concentrated under reduced pressure. After drying, 0.42 g of the product of Example 6 remained.
Method C)
Example 8 2-(4'-Chlorobiphenyl-4-sulfonylamino)-5-(2,3-dihydroindol-l-yl)-5-oxopentanoic acid
Stage 1: Reaction of Cbz-Glu anhydride with 2,3-dihydro-1H-indole 5 g (0.019 mol) of L-Cbz-glutamyl anhydride were dissolved in 100 ml of absolute dimethyl sulfoxide together with 2.56 ml (this corresponds to 0.023 mol) of 2,3-dihydro-1H-indole and the solution was stirred at room temperature (RT) for 30 min and at 4O°C for a further 30 min until the reaction was complete (TLC checking). The mixture was added to water, extracted with ethyl acetate a number of times, and the organic phase was washed with 1N HCI and with saturated NaCI solution, dried and concentrated. Yield: 7.07 g Stage 2: Removal of the Cbz protective group
6.8 g (0.018 mol) of the product from stage 1 were dissolved in 60 ml of a 33% strength HBr/glacial acetic acid solution in the course of 30 min and the solution was stirred at RT until the reaction was complete (about 15 h, TLC checking). The crude product obtained after concentration under reduced pressure was entrained a number of times with dichloromethane and taken up using methanol/water (10:1), and the product was filtered off, dried and again extracted by stirring in THF. After filtering off with suction and drying under reduced pressure, 2.13 g of the crystalline hydrobromide having a purity of 98% (according to HPLC) remained. The reductive removal of the protective group analogously to Example 12, stage 4, afforded the hydrochloride of 2-amino-5-(2,3-dihydroindoM-yl)-5-oxopentanoic acid in 85% yield.

Stage 3: 2-(4'-Chlorobiphenyl-4-sulfonylamino)-5-(2,3-dihydroindol-1-
yl)-5-oxopentanoic acid 0.33 g (1 mmol) of the product from stage 2 was dissolved in 60 ml of THF/water (1:1) and a pH of 12.0 was established using a titroprocessor and 0.5 N NaOH. Under constant pH, a solution of 0.345 g (1.2 mmol) of 4'-chlorobiphenyl-4-sulfonyl chloride, dissolved in 30 ml of THF, was added dropwise in the course of approximately 45 min. The mixture was stirred for a further 2 h at pH 12, (TLC checking), treated with 0.1 N HCI to pH 2, extracted a number of times with ethyl acetate, washed with water, dried and concentrated.
Yield: 0.395 g of melting point 182°C and a purity of more than 92%. The 4-chlorobiphenylsulfonic acid detectable as a slight impurity could be removed by chromatography or reprecipitation.
Example 12 5-(5-Fluoro-2,3-dihydroindol-l-yl)-5-oxo-2-(4'-pyrrolidin-l-yl-biphenyl-4-sulfonylamino) pentanoic acid
Stage 1: Biphenyl-4-ylpyrrolidine
10.0 g (0.043 mol) of 4-bromobiphenyl were suspended in 600 ml of
toluene together with 3.7 g (0.052 mol) of pyrrolidine and 6.2 g
(0.0642 mol) of sodium tertiary-butoxide and 900 mg of the catalyst
dichlorobis(tritolylphosphine)palladium(ll) were added. The mixture was
heated to reflux for 8 h, cooled, treated with water/ethyl acetate, and the
organic phase was washed with water, dried over sodium sulfate and
concentrated. The residue was dissolved in approximately 300 ml of
tertiary-butyl methyl ether and the hydrochloride of the product was
precipitated by addition of 50 ml of 1N ethereal HCI.
Yield: 6.0 g of melting point higher than 125°C (decomposition)
Stage 2: 4'-Pyrrolidin-1-ylbiphenyl-4-suIfonyl chloride
6 g (0.023 mol) of the product from stage 1 was introduced into 11 ml of
chlorosulfonic acid in portions with cooling and under protective gas and
the mixture was heated at 6O°C for 3.5 h. The solution was added to ice,
the suspension was brought to pH 8 by addition of solid sodium
bicarbonate, and the precipitated product was filtered off with suction and
dried under reduced pressure.
Yield: 7 g of melting point higher than 282 °C (decomposition).

Stage 3: Reaction of Z-Glu anhydride with 5-fIuro-2,3-dihydro-1H-
indole
8.8 g (0.0334 mol) of L-Z-glutamyl anhydride were dissolved in 150 ml of
absolute dimethyl sulfoxide together with 5.5 g (0.04 mol) of 5-fluoro-2,3-
dihydro-1H-indole and the solution was stirred at RT until the reaction was
complete (approximately 3 h, TLC checking). The mixture was added to
water, extracted a number of times with ethyl acetate and the organic
phase was washed with 1N HCI and with saturated NaCI, dried and
concentrated.
Yield: 13.2 g
Stage 4: Removal of the Z protective group
13 g (0,0325 mol) of the product from stage 3 were dissolved in 450 ml of
methanol and 33 ml of 1N HCI, a spatula tipful of palladium on carbon was
added, and the mixture was hydrogenated at 40 bar and 6O°C until the
reaction was complete (TLC checking). The crude product obtained after
filtration and concentration was taken up in THF under reflux, and the solid
hydrochloride which remained after cooling was filtered off and dried under
reduced pressure.
Yield: 7.1 g of melting point higher than 212°C
Stage 5: 5-(5-Fluoro-2,3-dihydroindol-1-yl)-5-oxo-2-(4'-pyrrolidin-1-
ylbiphenyl-4-sulfonylamino) pentanoic acid
0.303 g (1 mmol) of the product from stage 4 was dissolved in 60 ml of
THF/water (1:1) and a pH of 12.0 was established using a titroprocessor
(model 686, Metrohm) by means of 0.5 N NaOH. While keeping the pH
constant, a solution of 0.39 g (1.2 mmol) of the product from stage 2,
dissolved in 40 ml of THF, was added dropwise in the course of
approximately 45 min. The mixture was stirred for a further 2 h at pH 12
(TLC checking), treated with 0.1 N HCI until a pH of 2 was achieved and
extracted a number of times with ethyl acetate, and the extract was washed
with water, dried and concentrated. The product was purified by
chromatography on silica gel (dichloromethane/methanol 95:5).
Yield: 0.393 g of melting point 216 °C
Method D)
Example 15 Tromethamine salt of 2-(4'-chlorobiphenyl-4-sulfonylamino)-5-(2,3-dihydroindol-1 yl)-5-oxopentanoic acid
104.5 g (0.21 mol) of the compound according to Example 8 were suspended in 1000 ml of ethanol and warmed to 5O°C. 25.4 g of tromethamine (2-amino-2-hydroxymethyl-1,3-propanediol, TRIS) dissolved

in 100 ml of water were then added thereto. The mixture was briefly heated to reflux and filtered hot. The clear filtrate was allowed to cool to RTt whereupon the salt precipitated. It was filtered off with suction and washed twice with 150 ml of ethanol/water in a ratio of 9 to 1 each time and dried in a desiccator. Yield: 93.9 g (72%) of tromethamine salt
Example 16 Tris-(2-hydroxyethyl)ammonium 2-(4'-chlorobiphenyl-4-
sulfonylamino)-5-(2,3-dihydroindol-1-yl)-5-oxopentanoate 0.05 g of the product from Example 8 was dissolved in 3.18 g of acetone, 0.01 g of triethanolamine was added, and the solution was filtered and placed in an open Erlenmeyer vessel in a TLC chamber whose bottom was covered to approximately 1 cm with tert-butyl methyl ether. Crystal formation commenced after approximately 10 h and was complete after 4 to 5 days. The supernatant solvent was decanted off and the product obtained was dried under reduced pressure. Yield: 0.06 g of melting point 120°C.
Examples 1 to 5 of Table 1 were prepared according to method A), Examples 6 and 7 according to method B) and Examples 8 to 14 according to method C). The starting material needed for the introduction of the arylsulfonyl side chain of Example 13 (4'-morpholin-4-ylbiphenyl-4-sulfonyl chloride) was prepared analogously to method C), stage 1.

Pharmacological examples
Preparation and determination of the enzymatic activity of the catalytic
domain of human stromelysin and of neutrophil collagenase.
The two enzymes - stromelysin (MMP-3) and neutrophil collagenase (MMP-8) - were prepared according to Ye et al. (Biochemistry; 31 (1992) pages 11231-11235) or Weithmann et al. (Inflamm Res 46(1997) 246-252). For measurement of the inhibitory action on the enzymatic activity, 70 µl of buffer solution and 10 µl of enzyme solution were incubated for 15 minutes with 10 µl of a 10% strength (v/v) aqueous dimethyl sulfoxide solution which optionally contained the enzyme inhibitor. After addition of 10 µl of a 10% strength (v/v) aqueous dimethyl sulfoxide solution which contained 1 mmol/l of the substrate, the enzyme reaction was monitored by fluorescence spectroscopy (328 nm (ex)/393 nm (em)). The enzyme activity was shown as the extinction increase/minute. The IC50 values listed in Table 2 were determined as those inhibitor concentrations which in each case led to a 50% inhibition of the enzyme. The buffer solution contained 0.05% of Brij (Sigma, Deisenhofen, Germany) and 0.1 mol/l of piperazine-

N,N'-bis[2-ethane§ulfonic acid]/NaOH, 0.1 mol/l of NaCI, 0.01 mol/l of CaCI2 and 0.1 mol/I of piperazine-N,N'-bis[2-ethanesulfonic acid] (pH=6.5). The enzyme solution contained 5 µg/ml of one of the enzyme domains prepared according to Ye et al. The substrate solution contained 1 mmol/l of the fluorogenic substrate (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-3-(2',4'-dinitrophenyl)-L-2,3-diaminopropionyl-Ala-Arg-NH2 (Bachem, Heidelberg, Germany).

Determination of the solubility in water of the free acid of 2-(4'-chloro-biphenyl-4-sulfonylamino)-5-(5-fiuoro-2,3-dihydroindol-1-yl)-5-oxo-pentanoic acid according to Example 8 and its tromethamine salt according to Example 15
1. Materials employed 1.1 Test substances
a) Compound according to Example 8; 2-(4'-chlorobiphenyl-4-sulfonylamino)-5-(5-fluoro-2,3-dihydroindol-1-yl)-5-oxopentanoicacid

b) Compound according to Example 15; tromethamine salt of 2-(4'-chlorobiphenyl-4-sulfonylamino)-5-(5-fluoro-2,3-dihydroindol-1-yl)-5-oxopentanoic acid
1.2 Reagents
- Water: deionized, - Acetonitrile: LiChrosolv (Merck),
- Diethylamine: for synthesis (Merck), - Acetic acid: concentrated and 1N
(Merck)
2. Experimental procedure
2.1 2-(4^Chlorobiphenyl-4-sulfonylamino)-5-(5-fluoro-2,3-dihydroindol-l-
yl)-5-oxopentanoic acid
Approximately 50 mg of test substance according to 1.1a) were initially
introduced into a 100 ml Erlenmeyer flask. After addition of 10 mi of water,
the mixture was stirred at 25°C. After 2 hours (h) and after 3 ht aliquots
were taken and centrifuged. 1 ml each of the supernatant was diluted to
10 ml with mobile phase. The concentration of the active component was
determined by means of HPLC against an external standard.
Repetition of the experiment.
2.2 Tromethamine salt of 2-(4'-chlorobiphenyl-4-sulfonylamino)-5-(5-
fluoro-2,3-dihydroindol-1-yl)-5-oxopentanoic acid
Approximately 800 mg of the tromethamine salt according to 1.1b) were initially introduced into a 100 ml Erlenmeyer flask. After addition of 10 ml of water, the mixture was stirred at 25°C. After 2 h and after 3 h, aliquots were taken and centrifuged. 1 ml each of the supernatant was diluted to 100 ml with mobile phase. The concentration of the active component was determined by means of HPLC against an external standard. Repetition of the experiment.
3. Analysis
Instrument: Gynkotek HPLC unit
Column: Stationary phase: ChiraDex 5 µm, Merck
Dimensions: 250 mm x 4.0 mm
Mobile phase: Acetonitrile 440 ml; water 560 ml;
diethylamine 1 ml;
adjusted to pH 6.4 using acetic acid.
Injection volume: 10 µl
Flow: 0.8 ml/min
Column temperature: 4O°C
Detection: UVat26l nm

The time-dependent difference in the solubility values of 2-(4'-chloro-biphenyl-4-sulfonylamino)-5-(5-fluoro-2,3-dihydroindol-1-yl)-5-oxopentanoic acid could be attributable to supersaturation/saturation phenomena. As a result, the data value obtained after 3 h is used.
The water solubility of the tromethamine salt of 2-(4'-chlorobiphenyl-4-sulfonylamino)-5-(5-fluoro-2,3-dihydroindol-1-yl)-5-oxopentanoic acid is higher approximately by a factor of 600 than that of the free acid of 2-(4f-chlorobiphenyl-4-sulfonylamino)-5-(5-fluoro-2,3-dihydroindol-1-yl)-5-oxopentanoic acid.

WE CLAIM:
1. A compound of the formula 1

and/or a stereoisomeric form of the compound of the formula I and/or a physiologically tolerable salt of the compound of the
formula I, where R1 is
1. phenyl,
2. phenyl, which is mono- or disubstituted by
2.1. (C‹i-C6)-alkyl, which is linear, cyclic or branched,
2.2. hydroxyl,
2.3. (Ci-C6)-alkyl-C(0)-0-,
2.4. (Ci-C6)-alkyl-0-,
2.5. (Ci-Ce)-alkyl-0-(Ci-C4)-alkyK)-,
2.6. halogen,
2.7. -CF3,
2.8. -CN,
2.9. -N02,
2.10. HO-C(O)-,
2.11. (Ci-C6)-alkyl-0-C(0)-,
2.12. methylenedioxo,
2.13. R4-(R5 )N-C(O)-,
2.14. R4-(R5)N-, or
2.15. the radical of a heterocycle from the following group: isoxazolidine, morpholine, isothiazolidine, thio-morpholine, pyrazolidine, imidazolidine, piperazine, azetidine, pyrrole, pyrroline, pyrrolidine, pyridine, azepine, piperidine, oxazole, isoxazole, imidazole, pyrazole, thiazole, isothiazole, diazepine, thiomorpholine, pyrimidine and pyrazine, which is unsubstituted or substituted as described for R1 under 2.1 to 2.14, or
3. a heteroaromatic from the following group 3.1. to 3.15.,
which is unsubstituted or substituted as described for
R1 under 2.1 to 2.14,

3.i. pyrrole,
3.2. pyrazole,
3.3. imidazole,
3.4. triazole,
3.5. thiophene,
3.6. thiazole,
3.7. oxazole,
3.8. isoxazole,

3.9. pyridine,
3.10. pyrimidine,
3.11. indole,
3.12. benzothiophene,

3.13. benzimidazole,
3.14. benzoxazole or
3.15. benzothiazole,
2 4 5
R , R and R are identical or different and are
1. a hydrogen atom,
2. (Ci-C6)-alkyl-,
3. HO-C(OHCi-C6)-alkyl-,
4. phenyl-(CH2)n-, in which phenyl is unsubstituted or
mono- or disubstituted as described for R1 under 2.1.
to 2.14. and n is the integer zero, 1 or 2, or
5. picolyl or
6. R and R , together with the ring amino group, form a
4- to 7-membered ring in which, if appropriate, one of
the carbon atoms is replaced by -0-, -S- or -NH-,
R3is
1. -(Ci-C4)-alkyl-C(0)-N(R6)-R7. in which R6 and R7
together with the nitrogen to which they are bonded form a radical of the subformula Ha, lib or He

where in the subformulae lla( lib and lie
q is the integer zero, 1 or 2 and
r is the integer zero or 1, Z is a carbon, nitrogen, oxygen or sulfur atom or a
covalentbond, and R is a hydrogen atom or has the meaning described for
R1 above under 2.1 to 2.14,
2. -(Ci-C4)-alkyl-C(0)-Y,
in which Y is a radical of the subformula He or lid

where in the subformulae He and lid
o
R is a hydrogen atom or has the meaning described for R above under 2.1 to 2.14 and
R9 'S
2.1 a hydrogen atom
2.2 (Ci-Ce)-alkyh
2.3 HO-C(0)-(Ci-C6)-alkyh
2.4 'phenyl-(CH2)n-» in which phenyl is unsubstituted
or mono- or disubstituted as described for R under 2.1 to 2.14 and n is the integer zero, 1 or 2, or
2.5 picolyl, or
3. -(Ci^C4)-alkyl-C(0)-N(R9HCH2)0-N(R4)-R5 , where
g
R has the abovementioned meaning,
o is the integer 2, 3, 4 or 5 and
4 5
R and R together with the ring amino group form a 4- to 7-membered ring
from the group consisting of isoxazolidine, morpholine, isothiazolidine,
thiomorpholine, pyrazolidine, imidazolidine, piperazine, acetidine,
Dvrroline. azeoine. oioeridine and diazeoine

A is a) a covalent bond,
b) -0-,
c) -CH=CH- or
d) -CsC-,
B is a) -(CH2)m-. in which m is the integer zero, 1, 2, 3, 4, 5 or
6,
b) -O-(CH2)p, in which p is an integer from 1 to 5, or
c) -CH=CH-and
X is -CH=CH-, an oxygen atom or a sulfur atom, with the exception of the compounds
2-(biphenyl-4-sulfonylamino)-5-(2,3-dihyroindol-1-yl)-5-oxo-pentanecarboxylicacid and 5-(2,3-dihydroindol-1-yl)-5-oxo-2-(4'-pyrrolidin-1-ylbiphenyl-4-sulfonylamino)pentanoic acid and with the exception of the case in which the subformula lib r is the integer zero and z is a covalent bond.
2. The compound of the formula I as claimed in claim 1, wherein R is 1. phenyl or
2. phenyl, which is monosubstituted by
2.1. (C1-C6)-alkyl-, in which alkyl is linear, cyclic or branched,
2.2. -OH,
2.3. (Ci-C6)-alkyl-C(O)-O-,
2.4. (C1-C6)-alkyl-O-,
2.5. (C1-C6)-alkyl-O-(C1-C4)-alkyl-0-,
2.6. halogen,
2.7. -CF3 or
2.8. R4-(R5 )N-,
2 4 5
R , R and R are identical or different and are
1. a hydrogen atom,
2. (Ci-C6)-alkyl-or
4 5
3. R and R together with the ring amino group form a
4- to 7-membered ring, in which, if appropriate, one of
the carbon atoms is replaced by -0-, -S- or -NH-,
R3is1. -(Ci-C4)-alkyl-C(0)-N(R6)-R7. in which R6 and R7
together with the nitrogen to which they are bonded form a radical of the subformula lla, where in the subformula lla
q is the integer zero or 1,
Z is a carbon, nitrogen, oxygen or sulfur atom, and
g
R is a hydrogen atom or has the meaning described for R above under 2.1 to 2.8, 2. -(Ci-C4)-alkyl-C(0)-Y, in which
Y is a radical of the subformula lie or lid

where in the subformulae lie and lid
8
R is a hydrogen atom or has the meaning described for R above under 2.1 to 2.8 and
g
R is a hydrogen atom, or 3. -(Ci-C4)-aIkyl-C(0)-N(R9).(CH2)o-N(R4)-R5 , where
g
R is a hydrogen atom,
o is the integer 2 or 3 and
4 5
R and R are identical or different and are
1. a hydrogen atom,
2. (C‹i-C6)-alkyl-or
4 5
3. R and R together with the ring amino group form a
4- to 7-membered ring from the group consisting of isoxazolidine, morpholine, isothiazolidine, thiomorpholine, pyrazolidine, imidazolidine, piperazine, acetidine, pyrroline, pyrrolidine, azepine, piperidine and diazepine,
A is a covalent bond or -0-,
B is a) -(CH2)m-, in which m is the integer zero, 1 or 2, or
b) -O-(CH2)p, in which p is the integer 1 or 2, and X is -CH=CH-.
3. The compound of the formula I as claimed in claim 1, wherein 1
R is 1. phenyl or
2. phenyl, which is monosubstituted by
2.1. chlorine,
2.2. bromine,
2.3. fluorine,
2.4. pyrrolidine or
2.5. morpholine, R is a hydrogen atom, R3is 1. -(Ci-C4)-alky|.C(0)-N(R6)-R7, in which R6 and R7
together with the nitrogen to which they are bonded
form a radical of the subformula Ma, where in the
subformula fla
q is the integer zero or 1,
Z is a carbon atom, and
Q
R is a hydrogen atom, chlorine, bromine or fluorine, 2. -(Ci-C4)-alkyl-C(0)-Y, in which

Y is a radical of the subformula He or lid where in the subformulae lie and lid
8 9
R and R are each a hydrogen atom, or
3. -(Ci-C4)-alkyl-C(0)-N(R9HCH2)o-N(R4)-R5 , where g
R is a hydrogen atom, o is the integer 2 or 3 and
4 5
R and R are identical or different and independently of one another are
1. a hydrogen atom,
2. phenyl or
3. morpholine, A is a covalent bond or -0-f
B is a covalent bond and X is -CH=CH-.
The compound of the formula l as claimed in claim 1 or 2, which is a
compound from the group consisting of 2-(biphenyl-4-
sulfonylamino)-4-(naphthalen-1-ylcarbamoyl)butyric acid,
2-(biphenyl-4-sulfonylamino)-4-(naphthalen-2-yl-carbamoyl)butyric
acid, 2-(biphenyl-4-sulfonylamino)-4-(2-phenylaminoethyl-
carbamoyl)butyric acid, 2-(4'-chlorobiphenyl-4-sulfonylamino)-4-(3-morpholin-4-yl-propylcarbamoyl)butyric acid, 4«(3-(4-(biphenyl-4-sulfonylamino)-4-carboxybutyrylamino)propyl)morpholin-4Hum trifluoroacetate, 2-(4•- chlorobiphenyl-4-sulfonylamino)-5-(2,3-dihydroindol-1-yl)-5-oxopentanoic acid, 2-(4•-bromobiphenyl-4-sulfonylamino)-5- (2,3- dihydroindoM-yl)-5-oxopentanoic acid, 2-(4'-chloro-biphenyl-4- sulfonylamino)-5-(5-fluoro-2,3-dihydroindol-1-
yl)-5-oxopentanoic acid, 2-(4•-bromobiphenyl-4-sulfonylamino)-5-(5~fluoro-2,3-dihydro- indol-1-yl)-5-oxopentanoic acid, 5-(5-fluoro-2,3-dihydroindol-1 -yl)-5- oxo-2-(4'-pyrrolidin-l -yl-biphenyl-4-sulfonylamino)pentanoic acid and 5-(5-fluoro-2,3-dihydroindol-1-yl)-2-(4'-morpholin-4-yl-biphenyl-4"Sulfonylamino)-5-oxopentanoicacid.
The compound of the formula I as claimed in claim 4, which is a 2-amino-2-hydroxymethyl-1,3-propanediol salt, trimethylamine salt, triethylamine salt, ethanolamine salt or triethanolamine salt.

6. A process for the preparation of the compound of the formula I, as claimed in one or more of claims 1 to 5, which comprises a) reacting an aminocarboxylic acid of the formula II

in which R is a hydrogen atom or an ester protective group which is customary in peptide chemistry, s is the integer zero, 1, 2 or 3, R10 is the radical -OR 2, in which R12 is an ester
protective group, which can be cleaved in the presence of R , or a hydrogen atom, or R is a radical -N(R )-R , in
6 7
which R and R are as defined in formula I, with a sulfonic acid derivative of the formula III

where R ,X,A and B are as defined in formula I and Y is a
13 13
halogen atom, imidazolyl or OR , in which R is a hydrogen
atom, (C-i-C6)-alkyl, phenyl or benzyl, which is optionally
substituted,
in the presence of a base or, if appropriate, of a dehydrating
agent to give a compound of the formula IV

in which R1, A, X, B, R2, s, R10 and R11 have the abovementioned meaning, and then removing a protective

group R possibly included in the radical R10 in the presence of the protective group R , and then introducing the radical
ß 7
-N(R )-R by appropriate activation, known from peptide
chemistry, of the carboxyl group, and subsequently removing the included protective group R and obtaining the
compound of the formula l, or
b) reacting an amino acid anhydride of the formula V

13 14
in which R is a hydrogen atom and R is an N-protective
13 14
group known from peptide chemistry, or R and R are a cyclic N-protective group, e.g. phthaiimido and s is as defined
above under process a),
with a primary or secondary radical -N(R )-R in the sense of
a ring opening to give an intermediate of the formula VI

in which R , R , s, R and R have the abovementioned meaning, where this ring opening, depending on the protective group and reaction conditions as a rule regioselectively yields the isomer shown in formula VI, which, if regioisomer mixtures occur, can be further enriched by crystallization or chromatography, then cleaving the included protective group R and/or R with release of the amine,
and then carrying out, as described under a), an N-sulfonation with a sulfonic acid derivative of the formula III which leads to a product of the formula I, or
c) separating a compound of the formula I prepared according to
process a) or b)t which on account of its chemical structure
occurs in enantiomeric forms, into the pure enantiomers by

salt formation with enantiomerically pure acids or bases, chromatography on chiral stationary phases or derivatization by means of chiral enantiomerically pure compounds such as amino acids, separation of the diastereomers thus obtained, and removal of the chiral auxiliary groups, or d) isolating the compound of the formula I prepared according to process a), b) or c) in free form or, if acidic or basic groups are present, converting it into physiologically tolerable salts.
7. A pharmaceutical, which contains an efficacious amount of a
compound of the formula I as claimed in one or more of claims 1 to 5
together with a pharmaceutically suitable and physiologically
tolerable excipient, additive and/or other active compounds and
auxiliaries.
8. A process for the production of a pharmaceutical, which comprises
bringing at least one compound of the formula I as claimed in one or
more of claims 1 to 5 into a suitable administration form using a pharmaceutically suitable and physiologically tolerable vehicle and, if appropriate, further suitable active compounds, additives or auxiliaries.

Documents:

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Patent Number

213049

Indian Patent Application Number

IN/PCT/2001/610/CHE

PG Journal Number

13/2008

Publication Date

28-Mar-2008

Grant Date

19-Dec-2007

Date of Filing

02-May-2001

Name of Patentee

SANOFI-AVENTIS DEUTSCHLAND GMBH

Applicant Address

BRUNINGSTRASSE 50, D-65929 FRANKFURT,

Inventors:

#	Inventor's Name	Inventor's Address
1	SCHWAB, Wilfried	Schöne Aussicht, 49 D-65193 Wiesbaden,
2	SCHUDOK, Manfred	Eberlestrasse 28, D-65817 Eppstein,
3	HAASE, Burkhard	Konigsteiner Strasse 28, D-65719 Hofheim,
4	THORWART, Werner	Am Gansborn 3, D-65239 Hochheim,

PCT International Classification Number

C07C 311/19

PCT International Application Number

PCT/EP1999/007979

PCT International Filing date

1999-10-21

PCT Conventions:

#	PCT Application Number	Date of Convention	Priority Country
1	198 51 184.1	1998-11-06	Germany