| Title of Invention | "SEQUENCE OF A PORTION OF THE GENOME OF WHITESPOT SYNDROME VIRUS (WSSV) AFFECTING SHRIMP" |
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| Abstract | White spot syndrome virus (WSSV) is the major shrimp viral pathogen of Asia which has causes serious losses to the shrimp culture industry in several Asian countries. The invention provides sequence information on the WSSV which enables development of diagnostics based on Polymerase Chain Reaction (PCR), which are highly specific for WSSV and much more sensitive compared to PCR based on the WSSV sequence information known in the art. |
| Full Text | FIELD OF INVENTION The invention relates to sequences of a portion of the genome of whites pot syndrome virus (WSSV) affecting shrimp. BACKGROUND Of 1NVENITON Whites pot syndrome virus (WSSV) affecting shrimp has been the major shrimp viral pathogen of Asia, which has caused serious losses to the shrimp culture industry in several Asian countries. WSSV can be present in a dormant or latent form in shrimp larvae or juveniles or adults without showing any clinical signs of disease. For disease management in aquaculture, it would be very essential to rapidly detect the virus in brood stock, larvae or in juveniles or sub adults. Conventional methods for WSSV are based on histopathology. This method lacks the sensitivity required for rapid detection of latent infections. Information on the sequence of the DNA of the virus is very essential for development of rapid diagnostic methods such as gene probes and polymers chain reaction (PCR) based methods. The genome of the virus is over 200kb and only partial information on the sequences is available from other countries. OBJECTS OF THE INVENTION An object of this invention is to provide sequence information of white spot syndrome virus which is necessary for development of diagnostic tests. Another object of this invention is to provide sequence information of WSSV which enables development of diagnostic tests capable of detecting very low levels of virus which may be present in animals before showing any clinical signs of infection or in animals/larvae which carry the virus without showing any clinical signs of infections. At the outset of the description, which follows, it is to be understood that the ensuing description only illustrates a particular form of this invention. However such a particular form is only an exemplary embodiment and is not intended to be taken restrictively to imply any limitation on the scope of the present invention. DESCRIPTION OF THE INVENTION Based on the sequence information, Polymers Chain Reaction (PCR) primers have been developed. PCR is a rapid, sensitive and specific DNA-based method for detection of pathogens. However, the success of PCR, its sensitivity and specificity are dependent on the oligonucleotide primers which are used for the reaction. The invention proposes nucleotide (DNA) sequence of WSSV occurring in India, which enables development of PCR based diagnostics. Diagnostics PCR developed on the basis of these sequences, have been compared with other sequences known in the art. The new oligonucleotide primers based on the sequence of the present invention are highly specific for WSSV and are much more sensitive compared to the PCR based on existing sequence information. The limit of detection is 1000 fold less viral particles (1000 fold more sensitivity) compared to other primers reported in art. WE CLAIM; 1. Nucleotide (DNA) sequences of a portion of the genome of whites pot syndrome virus (WSSV) occurring in India, as under 2. Nucleotide sequence of white spot syndrome virus (WSSV) as substantially described herein. |
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1136-DEL-2001-Abstract-(11-01-2008).pdf
1136-DEL-2001-Claims-(11-01-2008).pdf
1136-DEL-2001-Claims-(28-02-2008).pdf
1136-DEL-2001-Correspondence-Others-(11-01-2008).pdf
1136-del-2001-correspondence-others.pdf
1136-del-2001-description (complete).pdf
1136-DEL-2001-GPA-(11-01-2008).pdf
1136-DEL-2001-Others-(11-01-2008).pdf
| Patent Number | 216295 | |||||||||
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| Indian Patent Application Number | 1136/DEL/2001 | |||||||||
| PG Journal Number | 13/2008 | |||||||||
| Publication Date | 28-Mar-2008 | |||||||||
| Grant Date | 12-Mar-2008 | |||||||||
| Date of Filing | 08-Nov-2001 | |||||||||
| Name of Patentee | THE SECRETARY, DEPARTMENT OF BIOTECHNOLOGY | |||||||||
| Applicant Address | B-2, 7-8 FLOOR, CGO COMPLEX, LODHI ROAD, NEW DELHI-110003 | |||||||||
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| PCT International Classification Number | C12N 15/33 | |||||||||
| PCT International Application Number | N/A | |||||||||
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