Title of Invention	"AN HERBAL THERAPEUTIC PRODUCT"
Abstract	The present invention relates to an herbal therapeutic product for controlling diabetes mellitus comprising at least one hypoglycemic compound extracted from the pulp of fruit Eugenia Jambolina. The said product is prepared from the pulp of the fruit Eugenia jambolina comprising cleaning and drying the fruit, followed by deseeding the said fruit, soaking the de-seeded pulp in water, filtering the said mixture, washing the residue with water to extract all the active compounds, purifying the said concentrated product in the chromatography by using slurries of different columns. The said hypoglycemic compounds are then extracted to get the herbal therapeutic product to control the glucose level.

Title of Invention

"AN HERBAL THERAPEUTIC PRODUCT"

Abstract

The present invention relates to an herbal therapeutic product for controlling diabetes mellitus comprising at least one hypoglycemic compound extracted from the pulp of fruit Eugenia Jambolina. The said product is prepared from the pulp of the fruit Eugenia jambolina comprising cleaning and drying the fruit, followed by deseeding the said fruit, soaking the de-seeded pulp in water, filtering the said mixture, washing the residue with water to extract all the active compounds, purifying the said concentrated product in the chromatography by using slurries of different columns. The said hypoglycemic compounds are then extracted to get the herbal therapeutic product to control the glucose level.

Full Text	The present invention relates to an herbal therapeutic product for controlling diabetes mellitus comprising at least one hypoglycemic compound extracted from the pulp of a fruit from a species of genus Eugenia specifically Eugenia jambolina. The embodiment of the invention resides in the various compounds of the product and their mixture having immediate as well as longer lasting effects in controlling diabetes mellitus. The other embodiment of the invention resides in four active hypoglycemic compounds comprising the product of the subject invention. These four active hypoglycemic compounds individually have the varying degrees of hypoglycemic characteristics, however, the combination thereof have been found to be most effective. Still another embodiment of the invention resides in the discontinuity of such herbal therapeutic product for a predetermined period after controlling the diabetes of the patients as the hypoglycemic compounds of such products are having the property to control the glucose level, for longer durations. It has been found that the intake of doses can be postponed for 2-3 days after controlling the glucose level because of longer lasting effects of these hypoglycemic compounds. The source of carbohydrates in the diet has a significant influence on lipid(fat) metabolism in human being. An amount of sucrose is increased and the quantity of complex carbohydrates is decreased, the concentration of cholesterol and more particularly of triglycerides increases which has a correlation between blood lipid concentration and impaired glucose tolerance. One of the objects of the present invention is that the present invention is a 100% herbal product without any side effects. Another object of the present invention is to treat the defect in metabolism, by increasing the activity of the glucose utilizing enzymes in liver, muscles and adipose tissues. The present invention relates to the product obtained from the species of genus Eugenia having hypoglycemic effects in the blood sugar level. A process for the preparation of the herbal therapeutic product to control the glucose level, comprises cleaning and drying the fruit of a species of family Eugenia to remove extraneous material from the outermost layer of the said fruit. De-seeding the fruit and soaking the said de-seeded fruit in water under controlled cooled conditions overnight to retain the activity of hypoglycemic compounds in the said mixture of water and pulp of de-seeded fruit. It is followed by mixing the said mixture to get the mixture of a uniform consistency. DETAILED DESCRIPTION OF THE INVENTION: Accordingly, the present invention relates to an herbal therapeutic product controlling diabetes mellitus comprising at least one hypoglycemic compound extracted from the pulp of a species of the genus Eugenia, specifically Eugenia jambolina. The process for the preparation of such herbal product comprising cleaning and drying the fruit of a species of genus Eugenia of family Myrtaceae to remove the extraneous material from the outer layer of the said fruit, deseeding the said fruit, soaking the de-seeded pulp in water, mixing the said mixture of water and pulp and keeping it overnight in a controlled cooled conditions to maintain the activity of hypoglycemic compounds, filtering the said mixture by conventional manner, washing the residue with water to extract all the active compounds from the said mixture of water and pulp in the form of clear watery solution, reducing the water content by keeping the said clear watery solution in controlled freezing conditions by conventional means to get the residues in concentrated form, purifying the said concentrated product in a chromatography by using slurries of different columns, adding buffers in the said purified mixture present in the said chromatography to elute the mixture of hypoglycemic compounds The various steps involved in the aforementioned process are : (a) The fruit of Eugenia jambolina is cleaned and dried to remove the extraneous material from the outer layer of the said fruit; (b) the seed is removed from the said fruit by conventional manner and is kept separately. (c) the de-seeded pulp is soaked in (distilled) water and the same is mixed to get a uniform consistency of the liquid ; ,(d) the said mixture is kept overnight in a controlled cooled conditions preferably between 4-10 degrees centigrade; (e) the said mixture is filtered by conventional manner (f) the residue are washed with water to extract all the active compounds from the said pulp in the form of clear watery solution (g) the water content are reduced by keeping the said clear watery solution in freezing conditions by conventional means to get the residues in concentrated form; (h) the said concentrated product is purified by treating the same with slurries of solvents selected preferably from sephadex, diethyl amino ethyl cellulose, anion exchange resins or silica gel and distilled water in the chromatography; (i) buffers are added in the said purified mixture present in the chromatography to elute the hypoglycemic compounds from the said mixture. The water content from said mixture can be reduced optionally to get the end product in solid form . The water is extracted from the said mixture after the stage of filtration by means of lypholizer. The said pulp of Eugenia jambolina, is subjected to column chromatography for a period of 15 minutes -2 hours to obtain a hypoglycemic fraction at a slightly acidic pH of 5-7 preferably 6.0 to 6.5. The said hypoglycemic fraction is subjected to thin layer chromatography using silica gel to form at least 2 visible bands . The Rf value of all the four bands is between 0.1-1.0, wherein the Rf value of one visible band which is having highly active hypoglycemic compound is between 0.5 to 0.7 and the Rf value of less active hypoglycemic compounds is between 0.1-0.2 and 0.9-1.0. The pH of the column containing mixture of chemical compounds is maintained at pH of 6.0-8.0 preferably 6.5 to 7.5. The said buffer used in the chromatography is selected preferably from the phosphate buffer, citrate buffer or TRIS buffer. The pH of the said mixture of hypoglycemic compounds is to be maintained neutral. The said mixture of hypoglycemic compounds were further analyzed to .separate the active hypoglycemic compounds by means of thin layer chromatography to separate the different compounds in the form of separate bands. Such bands were further exposed to the iodine chamber for the clear demarcation of the individual compound. It has been found that out of 4 such hypoglycemic compounds present in the mixture, two compounds are having immediate effect in controlling and maintaining the blood sugar level , while other two hypoglycemic compounds have their effect after certain time. In the thin layer chromatography, the said mixture of hypoglycemic compounds are dropped in the lower most end of a silica gel coated plate to get the deposition of said hypoglycemic compounds in the form of bands on the said silica gel coated plate by running with a mixture of solvents for 2-3 hours in the ascending manner. The said solvents are n-butanol, acetic acid and water in the ratio of 5:1:4. The silica gel coated plate with bands of hypoglycemic compounds is dried in cooled conditions and is exposed to iodine chamber to get the clear demarcation of the different hypoglycemic compounds bands which are extracted from the silica gel coated plate along with the fractions of silica gel. The extracted hypoglycemic compound along with silica gel is mixed with water for the separation of said silica gel by centrifugation. The water used in the subject process is preferably distilled water. A study was conducted to determine the effect of a herbal therapeutic product prepared from the pulp extracted from the genus Eugenia on the blood glucose, liver, aorta, pancreas and heart. The results were found very effective with no side effects which is the main embodiment of the present invention. Various tests were conducted to analyze the effect of said product on testing the blood glucose level in fasting and in glucose tolerance test. The test was conducted on alloxan induced diabetic rabbit where the said product prepared from the process of subject invention was given to alloxan induced diabetic rabbit after drawing the blood from fasting rabbits. After giving the dose of said product, the blood was withdrawn again from said rabbit after 90 minutes. After that 2 grams of glucose in a water solution was given to that rabbit. The blood was again withdrawn after two hours of glucose feed. It was found that said mixture reduced the blood glucose level even after the glucose feed, which was due to the rise in insulin level in the blood. The said product was found to increase the activity of enzymes in liver, muscle and adipose tissue, which use glucose and thereby reduces blood glucose level. The said product was further found to reduce the level of lipids in blood which are responsible for heart diseases like total cholesterol, low density lipoprotein cholesterol, very low density lipoprotein cholesterol and triglycerides. At the same time it increases the level of favourable lipids for health such as high density lipoprotein cholesterol (HDLC). The said mixture of hypoglycemic compounds were found to be having no adverse effects on the liver and kidney functions. No abnormalities were found after conducting the histopathology of liver, aorta, pancreas and heart, when the patient was treated with the product of subject invention. Hence, the subject invention relates to a herbal therapeutic product to control the glucose level in diabetes mellitus and also minimizes the risk of heart diseases extracted from the pulp of a species from the genus Eugenia prepared from the process as herein before described, comprising said hypoglycemic compound or a group of said hypoglycemic compounds in the form of a product. EXAMPLES EXAMPLE 1: To prepare an herbal therapeutic product of present invention, 1 Kg of pulp of the fruit from the genus Eugenia is mixed in 500ml of distilled water and kept overnight in cooled conditions at 4°C , the said mixture is filtered by conventional manner, and the residue is washed twice or thrice to extract all the active compounds from the said mixture, the water from the resultant mixture is reduced by keeping the said resultant mixture in a lypholizer to get the residues in paste form, the chromatography of the said paste is done by washing the said paste with diethyl amino ethyl cellulose, a phosphate buffer is then added to elute the mixture of hypoglycemic compounds. The said hypoglycemic compounds were given in the form of solution mixed with water. EXAMPLE 2: An herbal therapeutic product of present invention is prepared by taking 500 grams of pulp of the fruit Eugenia Jambolina and mixing it in 200ml of distilled water which is kept overnight in cooled conditions at 4-10°C , the said mixture is filtered by conventional manner, and the residue is washed twice to extract all the active compounds from the said mixture, the water from the resultant mixture is extracted by keeping the said resultant mixture in a lypholizer to get the residues in paste form, the chromatography of the said paste is done by washing the said paste with a solvent sephadex, a phosphate buffer is then added to elute the mixture of hypoglycemic compounds, the said hypoglycemic compounds are dropped in very small quantity on the lower most end of a glass plate having silica coating on it, the said plate is placed in a glass chamber having solvents n-butanol, acetic acid and water in the ratio of 4:1:5, the bands of hypoglycemic compounds are deposited on the silica coated plate by the capillary action, the plate with the deposition of hypoglycemic compounds is removed from the said glass chamber and is dried in cooled condition, then the plate is exposed to the iodine chamber to get the clear demarcation of the different hypoglycemic compounds bands which are extracted by scratching them off from the plate in the form of powder along with silica gel abstracts, the same is mixed with water for the separation of said silica gel by centrifugation. WE CLAIM:- 1. An herbal therapeutic product for controlling diabetes mellitus comprising at least one hypoglycemic compound extracted from the pulp of species of Eugenia preferably Eugenia jambolina. 2. The herbal therapeutic product as claimed in claim 1, comprising four hypoglycemic compounds extracted from the pulp of species of Eugenia preferably Eugenia j ambolina. 3. The herbal therapeutic product as claimed in claim 1, wherein the said pulp of species Eugenia, preferably Eugenia Jambolina, is subjected to column chromatography for a period of 15 minutes to 2 hours to obtain a hypoglycemic fraction at a slightly acidic pH of 5-7 preferably 6-6.5. 4. herbal therapeutic product as claimed in claim 1, wherein the said hypoglycemic fraction is subjected to thin layer chromatography using silica gel to form at least 2 visible bands. 5. the herbal therapeutic product as claimed in claim 1, wherein the said hypoglycemic faction obtained from thin layer chromatography is subjected to identification of different bands by exposing the said fraction to an iodine chamber. 6. obtained have Rf value of between 0.1-1 .0. 7. 'J'fie herbal therapeutic product as claimed in claim 4, wherein the Rf value of highly active hypoglycemic compounds having immediate and longer lasting effects is between 0.5-0.7. 8. herbal therapeutic product as claimed in claim 4, wherein Rf value of less active hypoglycemic compounds is between 0.1-0.2 and 0.9-1.0. 9. herbal therapeutic product for controlling diabetes mellitus comprising at least one hypoglycemic compound extracted from the pulp of species of Eugenia preferably Eugenia jambolina, substantially as herein described with reference to accompanying examples.

Full Text

The present invention relates to an herbal therapeutic product for controlling diabetes mellitus comprising at least one hypoglycemic compound extracted from the pulp of a fruit from a species of genus Eugenia specifically Eugenia jambolina.
The embodiment of the invention resides in the various compounds of the product and their mixture having immediate as well as longer lasting effects in controlling diabetes mellitus.
The other embodiment of the invention resides in four active hypoglycemic compounds comprising the product of the subject invention. These four active hypoglycemic compounds individually have the varying degrees of hypoglycemic characteristics, however, the combination thereof have been found to be most effective.
Still another embodiment of the invention resides in the discontinuity of such herbal therapeutic product for a predetermined period after controlling the diabetes of the patients as the hypoglycemic compounds of such products are having the property to control the glucose level, for longer durations.
It has been found that the intake of doses can be postponed for 2-3 days after controlling the glucose level because of longer lasting effects of these hypoglycemic compounds.
The source of carbohydrates in the diet has a significant influence on lipid(fat) metabolism in human being. An amount of sucrose is increased and the quantity of complex carbohydrates is decreased, the concentration of cholesterol and more particularly of triglycerides increases which has a correlation between blood lipid concentration and impaired glucose tolerance.

One of the objects of the present invention is that the present invention is a 100% herbal product without any side effects.
Another object of the present invention is to treat the defect in metabolism, by increasing the activity of the glucose utilizing enzymes in liver, muscles and adipose tissues.
The present invention relates to the product obtained from the species of genus Eugenia having hypoglycemic effects in the blood sugar level.
A process for the preparation of the herbal therapeutic product to control the glucose level, comprises cleaning and drying the fruit of a species of family Eugenia to remove extraneous material from the outermost layer of the said fruit. De-seeding the fruit and soaking the said de-seeded fruit in water under controlled cooled conditions overnight to retain the activity of hypoglycemic compounds in the said mixture of water and pulp of de-seeded fruit. It is followed by mixing the said mixture to get the mixture of a uniform consistency.
DETAILED DESCRIPTION OF THE INVENTION:
Accordingly, the present invention relates to an herbal therapeutic product controlling diabetes mellitus comprising at least one hypoglycemic compound extracted from the pulp of a species of the genus Eugenia, specifically Eugenia jambolina.
The process for the preparation of such herbal product comprising cleaning and drying the fruit of a species of genus Eugenia of family Myrtaceae to remove the extraneous material from the outer layer of the said fruit, deseeding the said fruit, soaking the de-seeded pulp in water, mixing the said mixture of water and pulp and keeping it overnight in a controlled cooled conditions to maintain the activity of hypoglycemic compounds, filtering the

said mixture by conventional manner, washing the residue with water to extract all the active compounds from the said mixture of water and pulp in the form of clear watery solution, reducing the water content by keeping the said clear watery solution in controlled freezing conditions by conventional means to get the residues in concentrated form, purifying the said concentrated product in a chromatography by using slurries of different columns, adding buffers in the said purified mixture present in the said chromatography to elute the mixture of hypoglycemic compounds
The various steps involved in the aforementioned process are :
(a) The fruit of Eugenia jambolina is cleaned and dried to remove the
extraneous material from the outer layer of the said fruit;
(b) the seed is removed from the said fruit by conventional manner and is
kept separately.
(c) the de-seeded pulp is soaked in (distilled) water and the same is mixed
to get a uniform consistency of the liquid ;
,(d) the said mixture is kept overnight in a controlled cooled conditions preferably between 4-10 degrees centigrade;
(e) the said mixture is filtered by conventional manner
(f) the residue are washed with water to extract all the active compounds
from the said pulp in the form of clear watery solution
(g) the water content are reduced by keeping the said clear watery
solution in freezing conditions by conventional means to get the residues in
concentrated form;

(h) the said concentrated product is purified by treating the same with slurries of solvents selected preferably from sephadex, diethyl amino ethyl cellulose, anion exchange resins or silica gel and distilled water in the chromatography;
(i) buffers are added in the said purified mixture present in the chromatography to elute the hypoglycemic compounds from the said mixture.
The water content from said mixture can be reduced optionally to get the end product in solid form . The water is extracted from the said mixture after the stage of filtration by means of lypholizer.
The said pulp of Eugenia jambolina, is subjected to column chromatography for a period of 15 minutes -2 hours to obtain a hypoglycemic fraction at a slightly acidic pH of 5-7 preferably 6.0 to 6.5.
The said hypoglycemic fraction is subjected to thin layer chromatography using silica gel to form at least 2 visible bands . The Rf value of all the four bands is between 0.1-1.0, wherein the Rf value of one visible band which is having highly active hypoglycemic compound is between 0.5 to 0.7 and the Rf value of less active hypoglycemic compounds is between 0.1-0.2 and 0.9-1.0.
The pH of the column containing mixture of chemical compounds is maintained at pH of 6.0-8.0 preferably 6.5 to 7.5.
The said buffer used in the chromatography is selected preferably from the phosphate buffer, citrate buffer or TRIS buffer.
The pH of the said mixture of hypoglycemic compounds is to be maintained neutral.

The said mixture of hypoglycemic compounds were further analyzed to .separate the active hypoglycemic compounds by means of thin layer chromatography to separate the different compounds in the form of separate bands. Such bands were further exposed to the iodine chamber for the clear demarcation of the individual compound. It has been found that out of 4 such hypoglycemic compounds present in the mixture, two compounds are having immediate effect in controlling and maintaining the blood sugar level , while other two hypoglycemic compounds have their effect after certain time.
In the thin layer chromatography, the said mixture of hypoglycemic compounds are dropped in the lower most end of a silica gel coated plate to get the deposition of said hypoglycemic compounds in the form of bands on the said silica gel coated plate by running with a mixture of solvents for 2-3 hours in the ascending manner. The said solvents are n-butanol, acetic acid and water in the ratio of 5:1:4. The silica gel coated plate with bands of hypoglycemic compounds is dried in cooled conditions and is exposed to iodine chamber to get the clear demarcation of the different hypoglycemic compounds bands which are extracted from the silica gel coated plate along with the fractions of silica gel. The extracted hypoglycemic compound along with silica gel is mixed with water for the separation of said silica gel by centrifugation.
The water used in the subject process is preferably distilled water.
A study was conducted to determine the effect of a herbal therapeutic product prepared from the pulp extracted from the genus Eugenia on the blood glucose, liver, aorta, pancreas and heart. The results were found very effective with no side effects which is the main embodiment of the present invention.
Various tests were conducted to analyze the effect of said product on testing the blood glucose level in fasting and in glucose tolerance test.

The test was conducted on alloxan induced diabetic rabbit where the said product prepared from the process of subject invention was given to alloxan induced diabetic rabbit after drawing the blood from fasting rabbits. After giving the dose of said product, the blood was withdrawn again from said rabbit after 90 minutes. After that 2 grams of glucose in a water solution was given to that rabbit. The blood was again withdrawn after two hours of glucose feed. It was found that said mixture reduced the blood glucose level even after the glucose feed, which was due to the rise in insulin level in the blood.
The said product was found to increase the activity of enzymes in liver, muscle and adipose tissue, which use glucose and thereby reduces blood glucose level. The said product was further found to reduce the level of lipids in blood which are responsible for heart diseases like total cholesterol, low density lipoprotein cholesterol, very low density lipoprotein cholesterol and triglycerides. At the same time it increases the level of favourable lipids for health such as high density lipoprotein cholesterol (HDLC). The said mixture of hypoglycemic compounds were found to be having no adverse effects on the liver and kidney functions.
No abnormalities were found after conducting the histopathology of liver, aorta, pancreas and heart, when the patient was treated with the product of subject invention.
Hence, the subject invention relates to a herbal therapeutic product to control the glucose level in diabetes mellitus and also minimizes the risk of heart diseases extracted from the pulp of a species from the genus Eugenia prepared from the process as herein before described, comprising said hypoglycemic compound or a group of said hypoglycemic compounds in the form of a product.

EXAMPLES
EXAMPLE 1:
To prepare an herbal therapeutic product of present invention, 1 Kg of pulp of the fruit from the genus Eugenia is mixed in 500ml of distilled water and kept overnight in cooled conditions at 4°C , the said mixture is filtered by conventional manner, and the residue is washed twice or thrice to extract all the active compounds from the said mixture, the water from the resultant mixture is reduced by keeping the said resultant mixture in a lypholizer to get the residues in paste form, the chromatography of the said paste is done by washing the said paste with diethyl amino ethyl cellulose, a phosphate buffer is then added to elute the mixture of hypoglycemic compounds. The said hypoglycemic compounds were given in the form of solution mixed with water.
EXAMPLE 2:
An herbal therapeutic product of present invention is prepared by taking 500 grams of pulp of the fruit Eugenia Jambolina and mixing it in 200ml of distilled water which is kept overnight in cooled conditions at 4-10°C , the said mixture is filtered by conventional manner, and the residue is washed twice to extract all the active compounds from the said mixture, the water from the resultant mixture is extracted by keeping the said resultant mixture in a lypholizer to get the residues in paste form, the chromatography of the said paste is done by washing the said paste with a solvent sephadex, a phosphate buffer is then added to elute the mixture of hypoglycemic compounds, the said hypoglycemic compounds are dropped in very small quantity on the lower most end of a glass plate having silica coating on it, the said plate is placed in a glass chamber having solvents n-butanol, acetic acid and water in the ratio of 4:1:5, the bands of hypoglycemic compounds are deposited on the silica coated plate by the capillary action, the plate with the deposition of hypoglycemic compounds is removed from the said glass chamber and is dried in cooled condition, then the plate is exposed to the iodine chamber to

get the clear demarcation of the different hypoglycemic compounds bands which are extracted by scratching them off from the plate in the form of powder along with silica gel abstracts, the same is mixed with water for the separation of said silica gel by centrifugation.

WE CLAIM:-
1. An herbal therapeutic product for controlling diabetes mellitus comprising at least one hypoglycemic compound extracted from the pulp of species of Eugenia preferably Eugenia jambolina.
2. The herbal therapeutic product as claimed in claim 1, comprising four
hypoglycemic compounds extracted from the pulp of species of Eugenia preferably
Eugenia j ambolina.
3. The herbal therapeutic product as claimed in claim 1, wherein the said pulp
of species Eugenia, preferably Eugenia Jambolina, is subjected to column
chromatography for a period of 15 minutes to 2 hours to obtain a hypoglycemic
fraction at a slightly acidic pH of 5-7 preferably 6-6.5.
4. herbal therapeutic product as claimed in claim 1, wherein the said
hypoglycemic fraction is subjected to thin layer chromatography using silica gel to
form at least 2 visible bands.
5. the herbal therapeutic product as claimed in claim 1, wherein the said
hypoglycemic faction obtained from thin layer chromatography is subjected to
identification of different bands by exposing the said fraction to an iodine chamber.
6. obtained have Rf value of between 0.1-1 .0.
7. 'J'fie herbal therapeutic product as claimed in claim 4, wherein the Rf value of
highly active hypoglycemic compounds having immediate and longer lasting effects
is between 0.5-0.7.
8. herbal therapeutic product as claimed in claim 4, wherein Rf value of less
active hypoglycemic compounds is between 0.1-0.2 and 0.9-1.0.

9. herbal therapeutic product for controlling diabetes mellitus comprising at least one hypoglycemic compound extracted from the pulp of species of Eugenia preferably Eugenia jambolina, substantially as herein described with reference to accompanying examples.

Documents:

475-del-2000-abstract.pdf

475-del-2000-claims.pdf

475-del-2000-correspondence-others.pdf

475-del-2000-correspondence-po.pdf

475-del-2000-description (complete).pdf

475-del-2000-form-1.pdf

475-del-2000-form-13.pdf

475-del-2000-form-18.pdf

475-del-2000-form-2.pdf

475-del-2000-form-3.pdf

475-del-2000-form-5.pdf

475-del-2000-gpa.pdf

475-del-2000-petition-137.pdf

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Patent Number

216784

Indian Patent Application Number

475/DEL/2000

PG Journal Number

13/2008

Publication Date

31-Mar-2008

Grant Date

19-Mar-2008

Date of Filing

01-May-2000

Name of Patentee

INDIAN COUNCIL OF MEDICAL RESEARCH

Applicant Address

ANSARI NAGAR, POST BOX 4911, NEW DELHI-110029, INDIA.

Inventors:

#	Inventor's Name	Inventor's Address
1	MURTHY POTHAPRAGADA SURYANARAYANA	B-164, SECTOR-14, NOIDA-201 301, UTTAR PRADESH
2	SHARMA SUMAN BALA	BIOCHEMISTRY, UNIVERSITY COLLEGE OF MEDICAL SCIENCE, GURU TEG BAHADUR HOSPITAL, DELHI-110095.
3	PRABHU KRISHAN MADHAV	DEPARTMENT OF BIOCHEMISTRY, GURU TEG BAHADUR HOSPITAL DELHI-110095
4	AFREENA NASIR	DEPARTMENT OF BIOCHEMISTRY, GURU TEG BAHADUR HOSPITAL, DELHI-110095, INDIA.

PCT International Classification Number

A61P 5/50

PCT International Application Number

N/A

PCT International Filing date

PCT Conventions:

#	PCT Application Number	Date of Convention	Priority Country
1			NA