Title of Invention	"A PROCESS FOR THE PREPARATION OF MOSQUITO LARVICIDAL FORMULATIONS BASED ON RABDOSIA MELISSOIDES INGREDIENTS"
Abstract	The invention relates to the preparation of Emulsifiable Concentrate formulation of the essential oil ofRabdosia metissoides Benth (Syn. Plectranthus melissoides Benth) or its chemical ingredients such as thymol, p. cymene or others employing a formulation solvent such as cyclohexanone, aromax, iomax, xylene, ceenine etc. and ionic or non-ionic or blends of ionic, non-ionic and ionic and non-ionic surfactants, and homogenising the product to obtain the final concentrate which is used for pest control after dilution in water to the required strength.

Title of Invention

"A PROCESS FOR THE PREPARATION OF MOSQUITO LARVICIDAL FORMULATIONS BASED ON RABDOSIA MELISSOIDES INGREDIENTS"

Abstract

The invention relates to the preparation of Emulsifiable Concentrate formulation of the essential oil ofRabdosia metissoides Benth (Syn. Plectranthus melissoides Benth) or its chemical ingredients such as thymol, p. cymene or others employing a formulation solvent such as cyclohexanone, aromax, iomax, xylene, ceenine etc. and ionic or non-ionic or blends of ionic, non-ionic and ionic and non-ionic surfactants, and homogenising the product to obtain the final concentrate which is used for pest control after dilution in water to the required strength.

Full Text	3. Description of invention Field of invention This invention relates to a process for the preparation of a novel mosquito larvicidnl formulation based on essential oil of the plant 'Patti' Rabdosia melissoides Benth or its phenolic or non-phenolic chemical constituents. It is in the field of pest control through the use of safe plant products. Background of invention In view of the various harmful effects of synthetic pesticides (mammalian toxicity, residues, pest resistance, pest resurgence etc.), the use of botanical pesticides is on the increase. Most botanicals are readily biodegradable and are reportedly devoid of the various harmful effects associated with use of the synthetics. 'Patti' Rabdosia melissoides Benth, (synonymous Plectranthus melissoides Bcnth), a wild weed plant which is abundantly available in the Tarai belt of Kumaon hills and other parts of India or elsewhere, has been found to be a source of biologically active materials. The essential oil as well as its various phenolic or non-phenolic constituents such as thymol, para-cymenc etc. obtained from the oil or other sources, are particularly effective against mosquito, for example, the larvae of Cnlex qiiinquefasciatus and others. These materials have a considerable use potential in domestic water air coolers and other situations where water stagnates over time. The present invention reports a process of incorporating these biologically active materials in a suitable formulation such as cmulsifiablc concentrate. In this form, the efficacy of the materials has been enhanced substantially. Prior art To the best of information, mosquito larvicidcs based on R. melissoides oil or its phenolic or non-phenolic constituents such as thymol, /wra-cymene and the like are non¬existent in any form. Objects of the invention An object of the invention is to propose a process for preparation of novel mosquito larvicidal formulations such as emulsifiable concentrate compositions based on the essential oil of R. melissoides or its phenolic or non-phenolic constituents, particularly thymol and para cymene. Another objective is to propose use of the developed compositions for control of pests, particularly mosquito larvae. The invention According to this invention, there is provided a process for preparation of novel mosquito larvicidal formulations such as emulsifiable concentrate based on the bioactive essential oil of Rabdosia melissoides Benth (Syn. Plcclranlhus melissoides Benth) or its chemical ingredients such as thymol, para cymene, and the likes, obtained from the essential oil or other sources, singularly or in combination, characterized by the dissolution (1-25% w/w) of the active material(s) along with an anionic or non-ionic or blends of ionic, non-ionic or both the ionic and non-ionic surfactants as emulsifier, in a formulation solvent and homogenizing the mix by physical or mechanical means, such as manual or mechanical shaking, stirring or the like, if necessary by slight warming, to yield a homogeneous product in uniform clear solution state, to be used as such or on emulsification in water or added to water bodies to form emulsions, to control pest. In accordance with this invention, the essential oil of R. melissoides or its phenolic or non-phenolic const ituent(s), particularly thymol or para cymene, is(are) dissolved in either of cyclohcxanone, aromax, iomax, ceeninc, naptha or xylene and the ionic, non-ionic or blends or ionic, non-ionic or the ionic and non-ionic surfactants (such as alkyl aryl sulphonates preferably calcium dodecylbenzene sulphonate, petroleum sulphonates, polyoxyethylenated alkyl phenols preferably nonyl phenols, alkyl aryl ethers, sorbitan or polyoxyethylenated sorbitan esters, polyoxyethylenated vegetable oils and the likes, as exemplified by commercial products such as Tweens, Spans, Hyoxid x 45, Emulsol MA, Atlox 3400 B, Unitox 33x, IGSURF 6000 or their likes), either alone or as blends (9:1 or 1:9), at levels of 1-15% w/w of the final product, are added; the volume made so as to obtain preferably 10-25% w/w of the essential oil/ingredicnt(s) and 5-10% of the surfactant in the final solution. The ingredients are homogenized and if necessary warmed (~ 60"C) to ensure dissolution before making the final volume/ weight to obtain a homogeneous product in a clear solution state. The product as obtained above is applied at the required concentration as such or on emulsification in water or addition to water bodies to yield emulsions or otherwise to control pest. It lias been found particularly effective in controlling acquatic insect pests such as mosquitoes. Further objects and advantages of this invention will be more apparent from the ensuing examples. Examples Preparation of 25% Emilsifiable Concentrate Take 25 g of R. melissoides essential oil and dissolve in commercial grade aromax. Add 5 g of Hyoxid x 45 (Aries Agrovet Industries) and mix thoroughly to obtain a homogeneous mass. Make volume with aromax to a final weight of 100 g and mix thoroughly. Preparation of 10% Kmulsifiable Concentrate Take 10 g of R. melissoides essential oil or thymol or para cymene or its other ingredients and dissolve in solvent ceenine. Add 10 g of a surfactant comprising of Tween 40 and Span 40 (9:1) and homogenize thoroughly. Add ceenine to make final weight of 100 g and mix thoroughly. Physico-chemical properties and bioactivity of some recipes The results on physico-chemical properties and bioactivity of a few selected recipes prepared in laboratory arc given in Table. Table. Physico-chemical properties and bioactivity of a few selected emulsifiable concentrates based on RahJosia melissoides essential oil or its constituent(s) (Table Removed) CL = Creamed layer, OL - Oily layer, SS = Solid 4. I/We claim 1. A process for the preparation of novel mosquito larvicidal formulation emulsifiable concentrate based on the bioactive essential oil of Rabdosia melissoides Benth (Syn. Plectranthus melissoides Benth) or its chemical ingredients such as thymol, para-cymene, and the likes, obtained from the essential oil or extracts of different parts of the same or similar plants, singularly or in combination, characterized by dissolution (1-25%, w/w) of the stated active material(s) along with anionic or non-ionic or blend of ionic, non-ionic or both ionic and non-ionic surfactants as emulsifier, in a formulation solvent and homogenizing the mix by physical or mechanical means, if needed by slight warming, to yield a homogeneous product in uniform clear solution state, to be used as solution or on emulsification in water or addition to water bodies to form emulsion, to control pest. 2. A process for the preparation of novel mosquito larvicidal formulation of claim 1 wherein the formulation solvent is either of cyclohexanone, aromax, iomax, xylene, naptha, ceenine and the like, preferably aromax or iomax or ceenine; 3. A process for the preparation of novel mosquito larvicidal formulation of claim 1 wherein the ionic, non-ionic or the blends of ionic, non-ionic and ionic or non- ionic surfactants may comprise alkyl aryl sulphonates preferably calcium dodecylbenzene sulphonate, petroleum sulphonates, polyoxyethylenated alkyl phenols preferably nonyl phenol, alkyl aryl ethers, sorbitan or polyoxyethylenated sorbitan esters, polyoxyethylenated vegetable oils and the like, either alone or as blends at levels of 1-15% w/w of the final product; preferably 5-15 per cent. 4. A process for the preparation of novel mosquito larvicidal formulation of claims 1 and 3 wherein the blends of surfactants, whenever used, comprise 9:1 to 1 :9 of the ingredients employed in the blend. 5. A process for the preparation of novel mosquito larvicidal formulation of claim 1 wherein the mix is homogenized by physical or mechanical means such as, manual or mechanical shaking, stirring, or the like, if necessary by slight warming (~60°C), to ensure dissolution and a homogeneous product in a clear solution state. 6. A process for the preparation of novel mosquito larvicidal formulation as claimed in claims 1 to 5 and herein described with reference to given examples so as to yield novel mosquito larvicidal preparations to be used as solution or on emulsification in water or addition to water bodies to from emulsion, to control pest.

Full Text

3. Description of invention Field of invention
This invention relates to a process for the preparation of a novel mosquito larvicidnl formulation based on essential oil of the plant 'Patti' Rabdosia melissoides Benth or its phenolic or non-phenolic chemical constituents. It is in the field of pest control through the use of safe plant products.
Background of invention
In view of the various harmful effects of synthetic pesticides (mammalian toxicity, residues, pest resistance, pest resurgence etc.), the use of botanical pesticides is on the increase. Most botanicals are readily biodegradable and are reportedly devoid of the various harmful effects associated with use of the synthetics.
'Patti' Rabdosia melissoides Benth, (synonymous Plectranthus melissoides Bcnth), a wild weed plant which is abundantly available in the Tarai belt of Kumaon hills and other parts of India or elsewhere, has been found to be a source of biologically active materials. The essential oil as well as its various phenolic or non-phenolic constituents such as thymol, para-cymenc etc. obtained from the oil or other sources, are particularly effective against mosquito, for example, the larvae of Cnlex qiiinquefasciatus and others. These materials have a considerable use potential in domestic water air coolers and other situations where water stagnates over time. The present invention reports a process of incorporating these biologically active materials in a suitable formulation such as cmulsifiablc concentrate. In this form, the efficacy of the materials has been enhanced substantially.
Prior art
To the best of information, mosquito larvicidcs based on R. melissoides oil or its phenolic or non-phenolic constituents such as thymol, /wra-cymene and the like are non¬existent in any form.
Objects of the invention
An object of the invention is to propose a process for preparation of novel mosquito larvicidal formulations such as emulsifiable concentrate compositions based on the essential oil of R. melissoides or its phenolic or non-phenolic constituents, particularly thymol and para cymene.
Another objective is to propose use of the developed compositions for control of pests, particularly mosquito larvae.
The invention
According to this invention, there is provided a process for preparation of novel mosquito larvicidal formulations such as emulsifiable concentrate based on the bioactive essential oil of Rabdosia melissoides Benth (Syn. Plcclranlhus melissoides Benth) or its chemical ingredients such as thymol, para cymene, and the likes, obtained from the essential oil or other sources, singularly or in combination, characterized by the dissolution (1-25% w/w) of the active material(s) along with an anionic or non-ionic or blends of ionic, non-ionic or both the ionic and non-ionic surfactants as emulsifier, in a formulation solvent and homogenizing the mix by physical or mechanical means, such as manual or mechanical shaking, stirring or the like, if necessary by slight warming, to yield a homogeneous product in uniform clear solution state, to be used as such or on emulsification in water or added to water bodies to form emulsions, to control pest.
In accordance with this invention, the essential oil of R. melissoides or its phenolic or non-phenolic const ituent(s), particularly thymol or para cymene, is(are) dissolved in either of cyclohcxanone, aromax, iomax, ceeninc, naptha or xylene and the ionic, non-ionic or blends or ionic, non-ionic or the ionic and non-ionic surfactants (such as alkyl aryl sulphonates preferably calcium dodecylbenzene sulphonate, petroleum sulphonates, polyoxyethylenated alkyl phenols preferably nonyl phenols, alkyl aryl ethers, sorbitan or polyoxyethylenated sorbitan esters, polyoxyethylenated vegetable oils and the likes, as exemplified by commercial products such as Tweens, Spans, Hyoxid x 45, Emulsol MA, Atlox 3400 B, Unitox 33x, IGSURF 6000 or their likes), either alone or as blends (9:1 or 1:9), at levels of 1-15% w/w of the final product, are added; the volume made so as to obtain preferably 10-25% w/w of the essential oil/ingredicnt(s) and 5-10% of the surfactant in the final solution. The ingredients are homogenized and if necessary warmed (~ 60"C) to ensure dissolution before making the final volume/ weight to obtain a homogeneous product in a clear solution state.
The product as obtained above is applied at the required concentration as such or on emulsification in water or addition to water bodies to yield emulsions or otherwise to control pest. It lias been found particularly effective in controlling acquatic insect pests such as mosquitoes.
Further objects and advantages of this invention will be more apparent from the ensuing examples.
Examples
Preparation of 25% Emilsifiable Concentrate
Take 25 g of R. melissoides essential oil and dissolve in commercial grade aromax. Add 5 g of Hyoxid x 45 (Aries Agrovet Industries) and mix thoroughly to obtain a homogeneous mass. Make volume with aromax to a final weight of 100 g and mix thoroughly.
Preparation of 10% Kmulsifiable Concentrate
Take 10 g of R. melissoides essential oil or thymol or para cymene or its other ingredients and dissolve in solvent ceenine. Add 10 g of a surfactant comprising of Tween 40 and Span 40 (9:1) and homogenize thoroughly. Add ceenine to make final weight of 100 g and mix thoroughly.
Physico-chemical properties and bioactivity of some recipes
The results on physico-chemical properties and bioactivity of a few selected recipes prepared in laboratory arc given in Table.
Table. Physico-chemical properties and bioactivity of a few selected emulsifiable concentrates based on RahJosia melissoides essential oil or its constituent(s)

(Table Removed)
CL = Creamed layer, OL - Oily layer, SS = Solid

4. I/We claim
1. A process for the preparation of novel mosquito larvicidal formulation
emulsifiable concentrate based on the bioactive essential oil of Rabdosia
melissoides Benth (Syn. Plectranthus melissoides Benth) or its chemical
ingredients such as thymol, para-cymene, and the likes, obtained from the
essential oil or extracts of different parts of the same or similar plants, singularly
or in combination, characterized by dissolution (1-25%, w/w) of the stated active
material(s) along with anionic or non-ionic or blend of ionic, non-ionic or both
ionic and non-ionic surfactants as emulsifier, in a formulation solvent and
homogenizing the mix by physical or mechanical means, if needed by slight
warming, to yield a homogeneous product in uniform clear solution state, to be
used as solution or on emulsification in water or addition to water bodies to form
emulsion, to control pest.
2. A process for the preparation of novel mosquito larvicidal formulation of claim 1
wherein the formulation solvent is either of cyclohexanone, aromax, iomax,
xylene, naptha, ceenine and the like, preferably aromax or iomax or ceenine;
3. A process for the preparation of novel mosquito larvicidal formulation of claim 1
wherein the ionic, non-ionic or the blends of ionic, non-ionic and ionic or non-
ionic surfactants may comprise alkyl aryl sulphonates preferably calcium
dodecylbenzene sulphonate, petroleum sulphonates, polyoxyethylenated alkyl
phenols preferably nonyl phenol, alkyl aryl ethers, sorbitan or polyoxyethylenated
sorbitan esters, polyoxyethylenated vegetable oils and the like, either alone or as
blends at levels of 1-15% w/w of the final product; preferably 5-15 per cent.
4. A process for the preparation of novel mosquito larvicidal formulation of claims 1
and 3 wherein the blends of surfactants, whenever used, comprise 9:1 to 1 :9 of the
ingredients employed in the blend.
5. A process for the preparation of novel mosquito larvicidal formulation of claim 1
wherein the mix is homogenized by physical or mechanical means such as,
manual or mechanical shaking, stirring, or the like, if necessary by slight warming
(~60°C), to ensure dissolution and a homogeneous product in a clear solution
state.
6. A process for the preparation of novel mosquito larvicidal formulation as claimed
in claims 1 to 5 and herein described with reference to given examples so as to
yield novel mosquito larvicidal preparations to be used as solution or on
emulsification in water or addition to water bodies to from emulsion, to control
pest.

Documents:

454-del-2001-abstract.pdf

454-del-2001-claims.pdf

454-del-2001-correspondence-others.pdf

454-del-2001-correspondence-po.pdf

454-del-2001-description (complete).pdf

454-del-2001-form-1.pdf

454-del-2001-form-19.pdf

454-del-2001-form-2.pdf

« Previous Patent

Next Patent »

Patent Number

218311

Indian Patent Application Number

454/DEL/2001

PG Journal Number

24/2008

Publication Date

13-Jun-2008

Grant Date

31-Mar-2008

Date of Filing

04-Apr-2001

Name of Patentee

INDIAN COUNCIL OF AGRICULTURAL RESEARCH

Applicant Address

KRISHI BHAWAN DR.RAJENDRA PRASAD ROAD NEW DELHI-110001,INDIA

Inventors:

#	Inventor's Name	Inventor's Address
1	DR. LALIT KUMAR	AGRICULTURAL CHEMICALS,INDIAN AGRICULTURAL RESEARCH INSTITUTE,NEW DELHI-10012,INDIA
2	DR. BALRAJ SINGH PARMAR	HEAD DIVISION OF CHEMICALS INDIAN AGRICULTURAL RESEARCH INSTITUTE,NEW DELHI-110012,INDIA

PCT International Classification Number

A01N 43/00

PCT International Application Number

N/A

PCT International Filing date

PCT Conventions:

#	PCT Application Number	Date of Convention	Priority Country
1			NA