Title of Invention	A SYNERGISTIC CHECKMITE COMPOSITION
Abstract	This invention relates a Check mite composition comprising: plant derived acaricidal agent - 0.01-0.1 %wt./vol. plant derived disinfectant agent - 0.1-3 %wt./vol. plant derived protein denaturant - 0.1-2 % wt./vol. fungistat agent - 0.1-3% wt./vol. the dispersing agent - 99.69-91.9% wt./vol

Full Text

This invention relates to a Check Mite composition, an environment friendly acaricide formulation for the control of house dust mite population in the domestic environment responsible for high incidence of respiratory allergies. BACKGROUND The major culprits for dust allergy are mites (Dermatophagoides farinae and D. pleronyssinus) prevalent in house dust. Universally, dust mites are minute co-inhabitants in almost every household and can not be seen with the naked eye. They are found in almost all home furnishing textiles and their favourite places are mattresses, cushions, carpets, upholstery and soft toys. The reactions in hypersensitive people range from itchy and watery eyes, repeated sneezes and running nose, cough and bronchial asthma to childhood eczema. The dust on which they thrive may comprise cotton, wool lint, animal and human dander, crumbs, pollens, molds, etc. House dust mites principally feed on human scales which are primarily found in mattresses, bedding, carpets etc. During occupation, the temperature and humidity of the human body provides an ideal microclimate in the mattresses for dust mites. Development from egg through larva, protonymph, tritonymph to adult requires about a month in cultures, under optimum conditions. An adult mite can live up to three months. Their food comprises of protein particles and fungi present in the dust. Mites may occasionally become airborne during bed-making. It has also been demonstrated that they secrete or release some allergens during bed-making. The allergen may comprise mites, eggs, dead mites and their excreta. A gram of dust mites may contain up to 1000 mites. Most particles of the faeces, whose physical properties are similar to pollen are deposited on the nasal mucosa and carried to the lungs causing localized inflammatory responses because of the high concentration of allergen. Control of mite population in the domestic environment is the best method ot preventing house dust allergy. The degree of cleanliness determines the number of house dust mites and the allergen level. Common control measures include vacuum cleaning, treating the carpets and bed spreads with insecticides, acaricides and fungicides. Reducing the mites population by interfering with the food chain has also been practised. However, a safe, environment friendly and effective formulation based on natural products for the control of house dust mite is not yet commercially available. PRIOR ART Few formulation are commercially available like Acardust, Acaroson, Allerbiocid etc., containing benzyl benzoate, the chief acaricide agent in these formulation is toxic at higher concentrations to humans as well a pets. As the effective concentration of benzyl benzoate used in these formulations is very high, its wide spread use as a domestic acaricide could be harmful. In one acaricidal formulation, derivatives of phenols in combination with several natural oils have been used as the active acaricide agent in combination with an antibiotic Natamycin as a fungicide. But the wide spread use of phenolic derivatives and essential oils is not nafe from physiological and odour point of view. Moreover, the fungicide as such can not destroy the mites. Apart from this, a few chemicals like benzyl alcohol, primiphos methyl, dibutyl phthalate, gama-hexachlorocyclohexane and diethyl-m-toluamide have been reported in literature as miticides. But from the toxicology and environmental safety point of view their use is not recommended. Accordingly, the object of this invention is to prepare a mite control composition for domestic use which should have the following characteristics: All the chemicals used should be safe from the toxicology point of view. • Should have multiple modes of action i.e., it should control the mite population, prevent the growth of fungi, reduce the existing allergen levels, act as a disinfectant as well as prevent the mites from developing resistance to these chemicals. • Should not have an offensive odour. Further, re-establishment of house dust mites after treatment with acaricides is the common problem due to the existence of nymph and eggs. Moreover, the miticide cannot reach the deeper layers of carpets and upholstery. Accordingly, the second • object of this invention is to control the mites and prevent its re-establishment by preparing the composition which can not only kill the adult mites but also be a ovicide and a larvicide. To achieve the said objectives this invention provides a process of preparing a Check Mite composition for the control of house dust mites comprising dispersing: plant derived acaricidal agent - 0.01-0.1 %wt./vol. plant derived disinfectant agent - 0.1-3 % wt./vol. plant derived protein denaturant - 0.1-2 % wt./vol. fungistat agent - 0.1-3% wt./vol. in the dispersing agent - 99.69-91.9% wt./vol The plant derived acaricidal agent is neem seed kernel extract containing azadirachtin / azadirachtin A of 2-90 % concentration and preferably of 20-35 % concentration. The neem seed kernel extract contains limonoids like nimbin, salannin, desacetylnimbin, desacetylsalannin, nimbandiol, azadirachtin-B and salannolacetate for preventing the mites from developing resistance against the active ingredient. The plant derived disinfectant agent is an alcoholic extract of resins like stryax benzoin and the plant derived protein denaturant is plant polyphenols like tannic acid, condensed tannins, phenolic compounds like gallic acid and phloroglucinol. The fungistat agents are fungicides used in food industry like natamycin, nipagin and the dispersing agents are ethanol, methanol and isopropyl alcohol. The ingredients viz. plant derived acaricidal agent, plant derived disinfectant agent, plant derived protein denaturant and fungistat agent of this composition are solids which are dissolved in an alcoholic solvent (dispersing agent) to give a clear pale brown coloured solution. This invention further relates to Check Mite composition which comprises: plant derived acaricidal agent - 0.01-0.1 %wt./vol. plant derived disinfectant agent - 0.1 -3 % wt./vol. plant derived protein denaturant - 0.1 -2 % wt./vol. fungistat agent - 0.1-3% wt./vol. the dispersing agent - 99.69-91.9% wt./vol The plant derived acaricidal agent is neem seed kernel extract containing azadirachtin / azadirachtin A of 2-90 % concentration and preferably of 20-35 % concentration. The neem seed kernel extract contains limonoids like nimbin, salannin, desacetylnimbin, desacetylsalannin, nimbandiol, azadirachtin-B and salannolacetate for preventing the mites from developing resistance against the active ingredient. The plant derived disinfectant agent is an alcoholic extract of resins like stryax, benzoin and the plant derived protein denaturant is plant polyphenols like tannic acid, condensed tannins, phenolic compounds like gallic acid, phloroglucinol. The fungistat agents are fungicides used in food industry like natamycin, nipagin and the dispersing agents are ethanol, methanol, isopropyl alcohol. Conclusion: Composition in Table 2 is very efficient in terms of speed of action and mite elimination. The total adult population is immobilized within an hour of treatment. A biweekly spray of 200 µl/100mg of the culture is required for 8 weeks to completely eliminate the population. Reestablishment on treated areas is totally prevented after 8 weeks. After eradication a biweekly prophylactic spray can contain population build up We claim: 1. A synergistic Check mite composition comprising: -plant derived acaricidal agent such as herein described - 0.01-0.1 % wt./voi. -plant derived disinfectant agent such as herein described - 0.1-3 % wt./vol. -plant derived protein denaturant such as herein described - 0.1 -2 % wt./vol. -fungistat agent such as herein described - 0.1-3% wt/vol. -the dispersing agent such as herein described - 99.69-91.9% wt./vol 2. A synergistic composition as claimed in claim 1 wherein the plant derived acaricidal agent is neem seed kernel extract containing azadirachtin of 2-90 % concentration. 3. A synergistic composition as claimed in claim 1 wherein the plant derived acaricidal agent is neem seed kernel extract containing azadirachtin / azadirachtin A preferably of 20-35 % concentration. 4. A synergistic composition as claimed in claim 2 wherein the neem seed kernel extract contains limonoids like nimbin, salannin, desacetylnimbin, desacetylsalannin, nimbandiol, azadirachtin-B and salannolacetate for preventing the mites from developing resistance against the active ingredient. 5. A synergistic composition as claimed in claim 1 wherein the plant derived disinfectant agent is an alcoholic extract of resins like stryax benzoin. 6. A synergistic composition as claimed in claim 1 wherein the plant derived protein denaturant is plant polyphenols like tannic acid, condensed tannins, phenolic compounds like gallic acid and phloroglucinol. 7. A synergistic composition as claimed in claim 1 wherein the fungistat agents are fungicides used in food industry like natamycin, nipagin. 8. A synergistic composition as claimed in claim 1 wherein the dispersing agents are ethanol, methanol, isopropyl alcohol. 9. A synergistic Check mite composition substantially as herein described with reference to the foregoing examples.

Documents:

827-mas-1998 abstract duplicate.pdf

827-mas-1998 abstract.pdf

827-mas-1998 claims duplicate.pdf

827-mas-1998 claims.pdf

827-mas-1998 correspondence others.pdf

827-mas-1998 correspondence po.pdf

827-mas-1998 description (complete) duplicate.pdf

827-mas-1998 description (complete).pdf

827-mas-1998 form-1.pdf

827-mas-1998 form-19.pdf

827-mas-1998 form-3.pdf

827-mas-1998 others.pdf