Indian Patents. 223705:"COMPOUND USEFUL AS INHIBITORS OF CRUZIPAIN AND OTHER CYSTEINE PROTEASES"

Title of Invention	"COMPOUND USEFUL AS INHIBITORS OF CRUZIPAIN AND OTHER CYSTEINE PROTEASES"
Abstract	Compounds of general formula (I) or general formula (II) wherein R1, P1, P2, Q, Y, (X)0, (W)n, (V)m, Z and U are as defined in the specification, are inhibitors of cruzipain and other cysteine protease inhibitors and are useful as therapeutic agents, for example in Chagas" disease, or for validating therapeutic target compounds.

Full Text	INHIBITORS OF CRUZIPAIN AND OTHER CYSTEINE PROTEASES T HIS INVENTION relates to compounds which are inhibitors of the protease cruzipain, a gene product of the Tiypanosoma cruzi parasite. In particular, the invention provides compounds that are useful for the therapeutic treatment of Tiypanosoma cnizi infection, to the use of these compounds, and to pharmaceutical compositions comprising them. Furthermore, this invention relates to compounds which are inhibitors across a broad range of cysteine proteases, to the use of these compounds, and to pharmaceutical compositions comprising them. Such compounds are useful for the therapeutic treatment of diseases in which participation of a cysteine protease is implicated. The trypanosomal family of parasites have a substantial worldwide impact on human and animal healthcare (McKerrow, J. H., et al, Ann. Rev. Microbiol. 47, 821-853, 1993). One parasite of this family, Trypanosoma cruzi, is the causative agent of Chagas' disease, which affects in excess of twenty million people annually in Latin and South America, is the leading cause of heart disease in these regions and results in more than 45,000 deaths per annum (Centers for Disease Control and prevention website). In addition, with the increase in migration of the infected population from rural to urban sites and movements from South and Central America into North America, the infection is spreading via blood transfusions, and at birth. The present treatments of choice for Tiypanosoma cruzi infection, nifurtimox and benznidazole (an NADH fumarate reductase inhibitor, Turrens, JF, et al, Mol Biochem Parasitol, 82(1), 125-9, 1996) are at best moderately successful, achieving ~60% cure during the acute phase of infection (see Docampo, R. Curr. Pham. Design, 7, 1157-1164, 2001 for a general discussion) whilst not being prescribed at all during the chronic phase where cardiomyopathy associated heart failure often occurs (Kirchhoff, L. V. New Engl. J. Med., 329, 639-644,1993). Additionally, these two drugs have serious adverse toxic effects, requiring close medical, supervision during treatment, and have been shown to induce chromosomal damage in chagastic infants (Gorla, N. B. et al, Mutat. Res. 206, 217-220, 1988). Therefore, a strong medical need exists for new effective drugs for the chemo therapeutic treatment si Tiypanosoma cruzi infection. Classically, the identification of enzymes found to be crucial for the establishment or propagation of an infectious disease has been instrumental in the development of successful drugs such as antivirals (e.g. HIV aspartyl protease inhibitors) and anti-bacterials (e.g. pMactam antibiotics). The search for a similar Achilles heel in parasitic infections has examined numerous enzymes (e.g. parasitic dihydrofolate reductase, see Chowdhury, S. F. et al, J. Med. Chem., 42(21), 4300-4312, 1999; trypanothione reductase, see Li, Z. et al, Bioorg. Med. Chem. Lett., 11 (2), 251-254, 2001; parasitic glyceraldehydes-3-phosphate dehydrogenase, see Aranov, A. M. et al, J. Med. Chem., 41(24), 4790-4799, 1998). A particularly promising area of research has identified the role of cysteine proteases, encoded by the parasite, that play a pivotal role during the life cycle of the parasite (McKerrow, J. H., et al, Bioorg. Med. Chem., 7, 639-644, 1999). Proteases form a substantial group of biological molecules winch to date constitute approximately 2% of all the gene products identified following analysis of several genome sequencing programmes (e.g. see Southan, C. J. Pept. Sci, 6, 453-458, 2000). Proteases have evolved to participate in an enormous range of biological processes, mediating their effect by cleavage of peptide amide bonds within the myriad of proteins found hi nature. This hydrolytic action is performed by initially recognising, then binding to, particular three-dimensional electronic surfaces displayed by a protein, which aligns the bond for cleavage precisely within the protease.catalytic site. Catalytic hydrolysis then commences through nucleophilic attack of the amide bond to be cleaved either via an amino acid side-chain of the protease itself, or through the action of a water molecule that is bound to and activated by the protease. Proteases in which the attacking nucleophile is the thiol side-chain of a Cys residue are known as cysteine proteases. The general classification of 'cysteine protease' contains many members found across a wide range of organisms from viruses, bacteria, protozoa, plants and fungi to mammals. Biological investigation of Trypanosoma cruzi infection has highlighted a number of specific enzymes that are crucial for the progression of the parasite's life cycle. One such enzyme, cruzipain, a cathepsin L-like cysteine protease, is a clear therapeutic target for the treatment of Chagas' disease ((a) Cazzulo, J. J. et al, Cwr. Pharm. Des. 7, 1143-1156, 2001; (b) Caffrey, C. R. etal, Cwr. Drug Targets, 1, 155-162, 2000). Although the precise biological role of cruzipain within the parasite's life cycle remains unclear, elevated cruzipain messenger RNA levels in the epimastigote developmental stage indicate a role in the nutritional degradation of host-molecules in lysosomal-like vesicles (Engel, J. C. et al, J. Cell. Sci, 111, 597-606,1998). The validation of cruzipain as a viable therapeutic target has been achieved with increasing levels of complexity. Addition of a general cysteine protease inhibitor, Z-Phe-Ala-FMK to Tiypanosoma cmzz-infected mammalian cell cultures blocked replication and differentiation of the parasite, thus arresting the parasite life cycle (Harth, G., et al, Mol Biochem. Parasitol 58, 17-24, 1993). Administration of a vinyl sulphone-based inhibitor in a Tiypanosoma crwzz-infected murine animal model not only rescued the mice from lethal infections, but also produced a complete recovery (Engel, J. C. et al, J. Exp. Med, 188(4), 725-734,1998). Numerous other in vivo studies have confirmed that infections with alternative parasites such as Leishmania major (Selzer, P. M. et al, Proc. Nat'l. Acad. Sci. U.S.A., 96, 11015-11022,1999), Schistosoma mansoni and Plasmodiumfalciparium (Olson, J. E. et al, Bioorg. Med. Chem., 7, 633-638, 1999) can be halted or cured by treatment with cysteine protease inhibitors. A variety of synthetic approaches have been described towards the design of cruzipain inhibitors. However, although providing a biological 'proof-of-principle' for the treatment of Trypanosoma cruzi infection, current inhibitors exhibit a number of biochemical and physical properties that may preclude their clinical development (e.g. see (a) Brinen, L. S. et al, Structure, 8, 831-840, 2000, peptidomimetic vinyl sulphones, possible adverse mammalian cell toxicity (see McKerrow, J. H. and Engel, J. unpublished results cited in Scheldt, K. A. et al, Bioorg. Med. Chem, 6, 2477-2494, 1998); (b) Du, X. et al, Chem. Biol, 7, 733-742, 2000, aryl ureas, generally with low uM activity, and high ClogP values, thus poor aqueous solubility and probably low oral bioavailability; (c) Roush, W. R. et al, Tetrahedron, 56, 9747-9762, 2000, peptidyl epoxysuccinates, irreversible inhibitors, with potent activity verses house-keeping mammalian proteases such as cathepsin B; (d) Li, R. et al, Bioorg. Med. Chem. 4(9), 1421-1427, 1996, bisarylacylhydrazides and chalcones, polyhydroxylated aromatics; (e) US6143931, WO 9846559, non-peptide a-ketoamides). Of the many different approaches to enzyme inhibition to date, only the cruzipain protease inhibitors have proven effective in curing disease-related animal models of Trypanosoma cruzi infection. Therefore, a clear medical need exists to progress these 'proof-of-principle' findings towards clinical candidates, suitable for human use, through the discovery of more efficacious cruzipain inhibitors that have a desirable combination of potency, selectivity, low toxicity and optimised pharmacokinetic parameters. Cruzipain and indeed many other crucial parasitic proteases belong to the papain-like CA Cl family and have close structural mammalian homologues. Cysteine proteases are classified into 'clans' based upon a similarity in the three-dimensional structure or a conserved arrangement of catalytic residues within the protease primary sequence. Additionally, 'clans' are further classified into 'families' in which each protease shares a statistically significant relationship with other members when comparing the portions of amino acid sequence which constitute the parts responsible for the protease activity (see Barrett, AJ et al, in 'Handbook of Proteolytic Enzymes', Eds. Barrett, A. J., Rawlings, N. D., and Woessner, J. F. Publ. Academic Press, 1998, for a thorough discussion). To date, cysteine proteases have been classified into five clans, CA, CB, CC, CD and CE (Barrett, A. J. et al, 1998). A protease from the tropical papaya fruit 'papain' forms the foundation of clan CA, which currently contains over 80 distinct and complete entries in various sequence databases, with many more expected from the current genome sequencing efforts. Proteases of clan CA/family Cl have been implicated in a multitude of disease processes e.g. human proteases such as cathepsin K (osteoporosis), cathepsin S (autoimmune disorders), cathepsin L (metastases) or parasitic proteases such as falcipain (malaria parasite Plasmodium falciparum), cruzipain (Trypanosoma cruzi infection). Recently a bacterial protease, staphylopain (S. aureus infection) has also been tentatively assigned to clan CA. X-ray crystallographic structures are available for a range of the above mentioned proteases in complex with a range of inhibitors e.g. papain (PDB entries, Ipad, Ipe6, Ipip, Ipop, 4pad, 5pad, 6pad, Ippp, Ithe, Icsb, Ihuc), cathepsin K (lauO, Iau2, Iau3, Iau4, latk, Imem, Ibgo, layw, layu), cathepsin L (Ics8), cathepsin S (currently on-hold, but published McGrath, M. E. et al, Protein Science, 7, 1294-1302,1998), cruzain (a recombinant form of cruzipain see Eakin, A. E. et al, 268(9), 6115-6118, 1993) (lewp, laim, 2aim, 1F29, 1F2A, 1F2B, 1F2C), staphylopain (Icv8). Each of the structures displays a similar overall active-site topology, as would be expected by their 'clan' and 'family' classification and such structural similarity exemplifies one aspect of the difficulties involved in discovering a selective inhibitor of cruzipain suitable for human use. However, subtle differences in terms of the depth and intricate shape of the active site groove of each CA Cl protease are evident, which may be exploited for selective inhibitor design. Additionally, many of the current substrate-based inhibitor complexes of CA Cl family proteases show a series of conserved hydrogen bonds between the inhibitor and the protease backbone, which contribute significantly to inhibitor potency. Primarily a bidentate hydrogen-bond is observed between the protease Gly66 (C=O)/ inhibitor N-H and the protease Gly66(NH)/inhibitor (C=O), where the inhibitor (C=O) and (NH) are provided by an amino acid residue NHCHRCO that constitutes the S2 sub-site binding element within the inhibitor (see Berger, A. and Schecter, I. Philos. Trans. R. Soc. Lond. [Biol.J, 257.249-264,1970 for a description of protease binding site nomenclature). A further hydrogen-bond between the protease main-chain (C=O) of asparagine or aspartic acid (158 to 163, residue number varies between proteases) and an inhibitor (N-H) is often observed, where the inhibitor (N-H) is provided by the SI sub-site binding element within the inhibitor. Thus, the motif X-NHCHRCO-NH-Y is widely observed amongst the prior art substrate-based inhibitors of CA Cl proteases. In the prior art, the development of cysteine protease inhibitors for human use has recently been an area of intense activity. Considering the CA Cl family members, particular emphasis has been placed upon the development of inhibitors of human cathepsins, primarily cathepsin K (osteoporosis), cathepsin S (autoimmune disorders) and cathepsin L (metastases), through the use of peptide and peptidomimetic nitriles (e.g. see WO-A-0109910, WO-A-0051998, WO-A-0119816, WO-A-9924460, WO-A-0049008, WO-A-0048992, WO-A-0049007, WO-A-0130772, WO-A-0055125, WO-A-0055126, WO-A-0119808, WO-A-0149288, WO-A-0147886), linear and cyclic peptide and peptidomimetic ketones (e.g. see Veber, D. F. and Thompson, S. K., Cuir. Opin. Diiig Discovery Dev., 3(4), 362-369,2000, WO-A-0170232, WO-A-0178734, WO-A-0009653, WO-A-0069855, WO-A-0029408, WO-A-0134153 to WO-A-0134160, WO-A-0029408, WO-A-9964399, WO-A-9805336, WO-A-9850533), ketoheterocycles (e.g. see WO-A-0055144, WO-A-0055124) and monobactams (e.g. see WO-A-0059881, WO-A-9948911, WO-A-0109169). The prior art describes potent in vitro inhibitors, but also highlights the many difficulties in developing a human therapeutic. For example, WO-A-9850533 and WO-A-0029408 describe compounds that may be referred to as cyclic ketones and are inhibitors of cysteine proteases with a particular reference towards papain family proteases and as a most preferred embodiment, cathepsin K. WO-A-9850533 describes compounds subsequently detailed in the literature as potent inhibitors of cathepsin K with good oral bioavailability (Witherington, J., 'Tetrahydrofurans as Selective Cathepsin K Inhibitors', RSC meeting, Burlington House, London, 1999). The compounds of WO-A-9850533 were reported to bind to cathepsin K through the formation of a reversible covalent bond between the tetrahydrofuran carbonyl and the active site catalytic cysteine residue (Witherington, J., 1999). Additionally, the same cyclic ketone compounds are described in WO-A-9953039 as part of a wide-ranging description of inhibitors of cysteine proteases associated with parasitic diseases, with particular reference to the treatment of malaria by inhibition of falcipain. However, subsequent literature describes the cyclic ketone compounds of WO-A-9850533 to be unsuitable for further development or for full pharmacokinetic evaluation due to a physiochemical property of the inhibitors, the poor chiral stability of the ct-aminoketone chiral centre (Marquis, R. W. et al, J. Med. Chem., 44(5), 725-736, 2001). WO-A-0069855 describes compounds that may also be referred to as cyclic ketones with particular reference towards inhibition of cathepsin S. The compounds of WO-A-0069855 are considered to be an advance on compounds of WO-A-9850533 due to the presence of the p-substituent on the cyclic ketone ring system that provides chiral stability to the ct-carbon of the cyclic ketone ring system. However, the compounds of WO-A-0069855 and indeed those of WO-A-9850533 describe a requirement for the presence of the potential hydrogen-bonding motif X- KHCHRCQ-NH-Y that is widely observed amongst the prior art substrate-based inhibitors of CA Cl proteases. It has now been discovered that certain compounds, defined by general formulae (I) and (II), are potent and selective cruzipain protease inhibitors which are useful in the treatment of Trypanosoma cmzi infection. Other compounds defined by general formulae (I) and (II) are protease inhibitors across a broad range of CA Cl cysteine proteases and compounds useful in the treatment of diseases caused by cysteine proteases. Compounds described by general formulae (I) and (II) do not contain the X-NHCHRCO-NH-Y motif that is widely observed amongst the prior art substrate- based inhibitors of CA Cl proteases, yet surprisingly compounds defined by general formulae (I) and (II) retain good potency. The present invention provides substituted tetrahydrofuro[3 ,2-6]pyrrol-3-one, tetrahydrothieno[3,2-Z>]pyrrol-3-one, hexahydropyrrolo[3 ,2-&]pyrrol-3-one, hexahydrocyclopenta[6]pyrrol-6-one, tetrahydrofuro[3,2-c]pyrazol-6-one, tetrahydrothieno[3,2-c]pyrazol-6-one, hexahydropyrrolo[3,2-c]pyrazol-6-ones hexahydrocyclopentapyrazol-6-one, hexahydrofuro[3,2-fc]pyridin-3-one, hexahydrothieno[3,2-fc]pyridin-3-one, octahydropyrrolo[3,2-fc]pyridin-3-one, octahydrocyclopenta[fc]pyridin-7-one, hexahydrofuro[3 ,2-c]pyridazin-7-one, hexahydrotbieno[3 ,2-c]pyridazin-7-one, octahydropyrrolo[3,2-c3pyridazin-7-one or octahydrocyclopenta[c]pyridazin-7-one bicyclic compounds defined by general formulae (I) and (II). Accordingly, the first aspect of the invention provides a compound according to general formula (I) or general formula (II): - wherein: R1 = C0-7-alkyl (when C = 0, R1 is simply hydrogen), Cs-e-cycloalkyl or Ar-Co-7-alkyl (when C = 0, R1 is simply an aromatic moiety Ar); Z = O, S, CR2R3 or NR4, where R4 is chosen from C0.ralkyl, C3.6-cycloalkyl or Ar-Co-7-alkyl; P1 = CR5R6, P2 = CR7R8, Q = CR9R10 orNR11, where Rn is chosen from C0-7~alkyl, C3.6-cycloalkyl or Ar-Co-7-alkyl; Each of R2, R3, R5, R6, R7, R8, R9 and R10 is independently chosen from Co-7-alkyl, C3^-cycloalkyl, Ar-Co-7-alkyl, O-C0-7-alkyl, O-C3^-cycloalkyl, O-Ar-CVralkyl, S-C0.7-alkyl, S-C3.6-cycloalkyl, S-Ar-Co-7-alkyl, NH-Q,. 7-alkyl, NH-C3^-cycloalkyl, NH-Ar-Co-7-alkyl, N(Co-7-alkyl)2, N(C3.6-cycloalkyl)2 orN(Ar-Co-7-alkyl)2; Y = CR12R13-CO, where R12, R13 are chosen from Co-7-alkyl, C3.6-cycloalkyl or Ar-Co-7-alkyl; (X)0 = CR14R15, where R14 and R15 are independently chosen from CQ.?-alkyl, Cs-e-cycloalkyl and Ar-Co-7-alkyl and o is a number from zero to three; (W)n = O, S, C(O), S(O) or S(O)2 or NR16, where R16 is chosen from Co-7-alkyl, C3.6-cycloalkyl and Ar-Co-7-alkyl and n is zero or one; (V)m = C(0), C(S), S(0), S(0)2, S(0)2NH, OC(O), NHC(O), NHS(O), NHS(0)2, OC(O)NH, C(0)NH or CR17R18, where R17 and R18 are independently chosen from Co-7-alkyl, C3.6-cycloalkyl, Ar-Co-y-alkyl and m is a number from zero to three, provided that when m is greater than one, (V)m contains a maximum of one carbonyl or sulphonyl group; U = a stable 5- to 7-membered monocyclic or a stable 8- to 11-membered bicyclic ring which is either saturated or unsaturated and which includes zero to four heteroatoms (as detailed below): wherein R19 is: Co-7-alkyl, C3^-cycloalkyl, Ar-Co-7-alkyl, O-Co-7-alkyl, O-C3.e-cycloalkyl, O-Ar-C0-7-alkyl, S-C0-7-alkyl, S-C3.6-cycloalkyl, S-Ar-C0-7-alkyl, NH-Co.7-alkyl, NH-C3.6-cycloalkyl, NH-Ar-C0-7-alkyl, N(C0.7-alkyl)2, N(C3.6-cycloalkyl)2 or N(Ar-Co-7-alkyl)2; or, when part of a CHR19 or CR19 group, R19 may be halogen; A is chosen from: CH2,CHR19sO,SandNR20; where R19 is as defined above; and R20 is chosen from: Co-7-alkyl, C3.6-cycloalkyl and Ar-C0-7-alkyl; B, D and G are independently chosen from: CR19, where R19 is as defined above, or N; E is chosen from: CH2, CHR19, O, S and NR20, where R19 and R20 are defined as above; J, L, M, R, T, T2, Ts and T4 are independently chosen from: CR19 and N, where R19 is as defined above; TS is chosen from: CHorN; q is a number from one to three, thereby defining a 5-, 6- or 7-membered ring. B, D, G, J, L, M, R, T, T2, T3 and T4 may additionally represent an N-oxide (N->O). The present invention includes all salts, hydrates, solvates, complexes and prodrugs of the compounds of this invention. The term "compound" is intended to include all such salts, hydrates, solvates, complexes and prodrugs, unless the context requires otherwise. Appropriate pharmaceutically and veterinarily acceptable salts of the compounds of general formulae (I) and (II) include salts of organic acids, especially carboxylic acids, including but not limited to acetate, trifluoroacetate, lactate, gluconate, citrate, tartrate, maleate, malate, pantothenate, adipate, alginate, aspartate, benzoate, butyrate, digluconate, cyclopentanate, glucoheptanate, glycerophosphate, oxalate, heptanoate, hexanoate, fumarate, nicotinate, pahnoate, pectinate, 3-phenylpropionate, picrate, pivalate, proprionate, tartrate, lactobionate, pivolate, camphorate, undecanoate and succinate, organic sulphonic acids such as methanesulphonate, ethanesulphonate, 2-hydroxyethane sulphonate, camphorsulphonate, 2-naphthalenesulphonate, benzenesulphonate, p-chlorobenzenesulphonate and p-toluenesulphonate; and inorganic acids such as hydrochloride, hydrobromide, hydroiodide, sulphate, bisulphate, hemisulphate, thiocyanate, persulphate, phosphoric and sulphonic acids. Salts which are not pharmaceutically or veterinarily acceptable may still be valuable as intermediates. Prodrugs are any covalently bonded compounds which release the active parent drug according to general formulae (I) or (II) in vivo. A prodrug may for example constitute an acetal or hemiacetal derivative of the exocyclic ketone functionality present in the tetrahydrofuro[3,2-6]pyrrol-3-one, tetrahydrothieno[3,2-6]pyrrol-3- one, hexahydropyrrolo[3,2-6]pyrrol-3-one, hexahydrocyclopenta[&]pyrrol-6-one, tetrahydrofuro[3,2-c]pyrazol-6-one, tetrahydrothieno[3,2-c]pyrazol-6-one, hexahydropyrrolo[3,2-c]pyrazol-6-one, hexahydrocyclopentapyrazol-6-one, hexahydrofuroES^-^pyridin-S-one, hexahydrothieno[3,2-6]pyridin-3-one, octahydropyrrolo[3,2-ft]pyridin-3-one, octahydrocyclopenta[6]pyridin-7-one, hexahydrofuro[3,2~c]pyridazin-7-one, hexahydrothieno[3,2-c]pyridazin-7-one, octahydropyrrolo[3,2-c]pyridazin-7-one or octahydrocyclopenta[c]pyridazin-7-one scaffold. If a chiral centre or another form of isomeric centre is present in a compound of the present invention, all forms of such isomer or isomers, including enantiomers and diastereoisomers, are intended to be covered herein. Compounds of the invention containing a chiral centre may be used as a racemic mixture, an enantiomerically enriched mixture, or the racemic mixture may be separated using well-known techniques and an individual enantiomer may be used alone. 'fjalogen' as applied herein is meant to include F, Cl, Br, I; 'Heteroatom' as applied herein is meant to include O, S and N; 'Co-7-alkyl' as applied herein is meant to include stable straight and branched chain aliphatic carbon chains containing zero (i.e. simply hydrogen) to seven carbon atoms such as methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, ,t-butyl, pentyl, isopentyl, hexyl, heptyl and any simple isomers thereof. Additionally, any Co-v-alkyl may optionally be substituted at any point by one, two or three halogen atoms (as defined above) for example to give a trifhioromethyl substituent. Furthermore, CQ.?-alkyl may contain one or more heteroatoms (as defined above) for example to give ethers, thioethers, sulphones, sulphonamides, substituted amines, amidines, guanidines, carboxylic acids, carboxamides. If the heteroatom is located at a chain terminus then it is appropriately substituted with one or two hydrogen atoms. A heteroatom or halogen is only present when Co-v-alkyl contains a minimum of one carbon atom. 'Cs-e-cycloalkyF as applied herein is meant to include any variation of 'Co-7-alkyl' which additionally contains a carbocyclic ring such as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl. The carbocyclic ring may optionally be substituted with one or more halogens (as defined above) or heteroatoms (as defined above) for example to give a tetrahydrofuran, pyrrolidine, piperidine, piperazine or morpholine substituent. ' Ar-Co-7-alkyl' as applied herein is meant to include any variation of Co-7-alkyl which additionally contains an aromatic ring moiety 'Ar'. The aromatic ring moiety Ar can be a stable 5 or 6-membered monocyclic or a stable 9 or 10 membered bicyclic ring which is unsaturated, as defined previously for U in general formulae (I) and (II). The aromatic ring moiety Ar may be substituted by R19 (as defined above for U in general formulae (T) and (II)). When C = 0 in the substituent Ar-Co-7-alkyl, the substituent is simply the aromatic ring moiety Ar. Other expressions containing terms such as alkyl and cycloalkyl are intended to be construed according to the definitions above. For example "CM alkyl" is the same as Co-7-alkyl except that it contains from one to four carbon atoms. If different structural isomers are present, and/or one or more chiral centres are present, all isomeric forms are intended to be covered. Enantiomers are characterised by the absolute configuration of their chiral centres and described by the R- and S- sequencing rules of Cahn, Ingold and Prelog. Such conventions are well known in the art (e.g. see 'Advanced Organic Chemistry', 3rd edition, ed. March, J., John Wiley and Sons, New York, 1985). Preferred compounds of general formulae (I) and (II) include, but are not limited to, those in which, independently or in combination: ZisO,S,NHorCH2; P1andP2areCH2;and QisCH2orNH. Also, preferred compounds of general formulae (I) and (II) include, but are not limited to, those in which, independently or in combination Z is MR4, where R4 is a Ar-Ci-4-alkyl or a substituted carbonyl or sulphonyl group; P1 andP2areCH2;and QisCH2orNH. Thus, examples of preferred compounds include those containing a bicyclic moiety chosen from tetrahydrofuro[3,2-6]pyrrol-3-one, tetrahydrothieno[3,2-6]pyrrol-3-one, hexahydropyrrolo[3,2-6]pyrrol-3-oue, hexahydrocyclopenta[6]pyrrol-6-one, tetrahydrofuro[3,2-c]pyrazol-6-one, tetrahydro-thieno[3,2-c]pyrazol-6-one, hexahydropyrrolo[3,2-c]pyrazol-6-one, hexahydro-cyclo-pentapyrazol-6-one, hexahydrofuro[3,2-6]pyridin-3-one, hexahydrothieno[3,2-6]pyridin-3-one, octahydropyrrolo[3,2-6]pyridin-3-one, octahydrocyclopenta[i]pyridin-7-one, hexahydrofuro[3,2-c]pyridazin-7-one, hexahydrothieno[3,2-c]pyridazin-7-one, octahydropyrrolo[3,2-c]pyridazin-7-one or octahydrocyclopenta[c]pyridazin-7-one as shown below. In compounds of general formulae (I) and (II), it is preferred that R1 comprises CQ.?-alkyl or Ar-Co-7-alkyl. Thus, for example, preferred R1 moieties include hydrogen, or a straight or branched alkyl chain, or a straight or branched heteroalkyl chain, or an optionally substituted arylalkyl chain, or an optionally substituted arylheteroalkyl chain. It is particularly preferred that R1 is hydrogen or Q-4 alkyl or Ar-Ci-4-alkyl and examples of such R1 substituents include, but are not limited to: where R19 is defined above. In preferred compounds of general formulae (I) and (II), Y is CHR13CO where R13 is selected from Co-7-alkyl or Ar-Co.7-alkyl, for example hydrogen, a straight or branched alkyl chain, a straight or branched heteroalkyl chain, an optionally substituted arylalkyl chain or an optionally substituted arylheteroalkyl chain. Additionally, in preferred compounds of general formulae (I) and (II), R13 is selected from Ca-e-cycloalkyl, for example cyclohexylmethyl. Examples of preferred Y substituents include the following: wherein R1, R2U and Ar are as defined above. More preferred R13 groups include Ci-4-alkyl, which may be substituted with OH, NR20R20, COOR20, or CONR20 or cycloalkylmethyl or Ar-CM-alkyl, where the aryl group may be substituted with R19; wherein each R19 and R20 is independently as defined above. Even more preferred R13 groups comprise Ar-CHb-, where the aromatic ring is an optionally substituted phenyl or monocyclic heterocycle. Additionally, even more preferred R13 groups comprise simple branched alkyl groups such as isobutyl or straight heteroaUcyl chains such as benzylsulfanylmethyl or benzylsulphonylmethyl. Furthermore, even more preferred R13 groups comprise cyclohexylmethyl. Examples of even more preferred Y substituents comprise the following, .19 wherein R and Ar are as defined previously It is preferred that in the group (X)0, each of R14 and R15 is selected from C0-7-alkyl or Ar-Co-7-alkyl, for example hydrogen, a straight or branched alkyl chain, a straight or branched heteroalkyl chain, an optionally substituted arylalkyl chain or an optionally substituted arylheteroalkyl chain. More preferred (X)0 groups comprise R14 chosen from hydrogen; R15 is Ci_4-alkyl, which may be substituted with OH, NR20R20, COOR20, or CONR20; or Ar-CM-alkyl, where the aryl group may be substituted with R19, wherein each R19 and R20 is independently as defined above. wherein R and R are as efined previously. Even more preferred ompounds of geeral formulae (I) and (II), comprise (X)0 groups that are simple alkyl groups such as methylene and where o = 0 or 1. In the group (W)n, W is preferably O, S, SO2, S(O), C(O) or NR16, where R16 is Chalky!; and n is 0 or 1. More preferred compounds of general formulae (I) and (II), comprise (W)n groups defined as O, S, SO2, C(O) and NH where n = 0 or 1. Yet even more preferred compounds of general formulae (I) and (II), comprise (W)n groups defined as NH where n = 1. In the group (V)m: V is preferably C(O), C(O)NH or CHR18, where R18 is Qn-alkyl; and m is 0 or 1. Preferred V and W substituent combinations encompassed by general formulae (I) and (II) include, but are not limited to: Additionally, a preferred V and W substituent combination encompassed by general formulae (I) and (II) includes, but is not limited to: More preferred V, W and X substituent combinations encompassed by general formulae (I) and (II) comprise, but are not limited to In preferred compounds of general formulae (I) and (II), U comprises an optionally substituted 5- or 6-membered saturated or unsaturated heterocycle or Ar group or an optionally substituted saturated or unsaturated 9- or 10-membered heterocycle or Ar group. Examples of such preferred U rings include the following: More preferred compounds of general formulae (I) and (II), contain a U group comprising of a bulky alkyl or aryl group at the para position of an aryl Ar. Also, more preferred compounds contain a meta or para-biaryl Ar-Ar, where Ar is as previously defined. Additionally, more preferred compounds contain a 6,6 or 6,5 or 5,6-fused aromatic ring. Examples of more preferred U groups are wherein R19, D, E, G, J, L, M, R, T, T2, T3 and T4 are as defined previously. Even more preferred compounds of general formulae (I) and (II), particularly for inhibition of cruzipain, contain a U group comprising a 6-membered Ar ring containing a bulky alkyl or aryl group at the para position, where Ar is as previously defined formulae (I) and (II), contain a U group comprising but are not limited to the following, wherein R19, D, E, G, J and L are as defined previously. Abbreviations and symbols commonly used in the peptide and chemical arts are used herein to describe compounds of the present invention, following the general guidelines presented by the TUP AC-TUB Joint Commission on Biochemical Nomenclature as described in Eur. J. Biochem., 158, 9-, 1984. Compounds of formulae (I) and (II) and the intermediates and starting materials used in then-preparation are named in accordance with the IUPAC rules of nomenclature in which the characteristic groups have decreasing priority for citation as the principle group. An example compound of formula (I), compound (1) in which R1 is H, PI is methylene, Q is methylene, Z is oxygen, Y is 4-methylpentyl, (X)o is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:- (3aS,6a£)4-(2S-Berizyloxy-4-methylpentano A second example compound of formula (I), compound (2) in which R1 is H, Pi is methylene, Q is methylene, Z is sulphur, Y is 4-methylpentyl, (X)o is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:- (2) (3aR,6aR) 4-(25-Benzyloxy-4-methylpentanoyl)tetrahydrothieno[3,2-6]pyrrol-3-one A third example compound of formula (I), compound (3) in R1 is H, PI is methylene, Q is methylene, Z is methylene, Y is 4-methylpentyl, (X)o is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:- l-(2S-Benzyloxy-4-methylpentanoyl)hexahydrocyclopenta[6]pyrrol-6-one A fourth example compound of formula (I), compound (4) in R1 is H, PI is methylene, Q is methylene, Z is NH, Y is 4-methylpentyl, (X)o is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:- (3 aS,6aR) 1 -(25-Ben2yloxy-4-methylpentauoyl)hexahydropyrrolo[3,2-6]pyrrol-3-one A fifth example compound of formula (I), compound (5) in which R1 is H, PI is methylene, Q is NH, Z is oxygen, Y is 4-methylpentyl, (X)0 is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:- (3a7?,6a5)4-(25'-Benzyloxy-4-methylpentanoyl)tetrahydrofuro[352-c]pyrazol-6-one A sixth example compound of formula (I), compound (6) in which R1 is H, PI is methylene, Q is NH, Z is sulphur, Y is 4-methylpentyl, (X)o is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:- (3a/?,6a/?)4-(25'-Benzyloxy-4-methylpentanoyl)tetrahydrothieno[3,2-c]pyrazol-6-one A seventh example compound of formula (I), compound (7) in R1 is H, PI is methylene, Q is NH, Z is methylene, Y is 4-methylpentyl, (X)o is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:- -(25-Benzyloxy-4-methylpentanoyl)hexahydrocyclopentapyrazol-6-one An eighth example compound of formula (I), compound (8) in R1 is H, PI is methylene, Q is NH, Z is NH, Y is 4-methylpentyl, (X)o is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:- (3a/?,6aS) l-(2iSr-Ben2yloxy-4-methylpentanoyl)hexahydropyrrolo[3,2-c]pyrazol-6-one An example compound of formula (II), compound (9) in which R1 is H, PI is methylene, ?2 is methylene, Q is methylene, Z is oxygen, Y is 4-methylpentyl, (X)o is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:- (3a5,7aR)4-(2iSr-Ben2yloxy-4-methylpentanoyl)hexahydrofuro[3,2-fe]pyridin-3-one A second example compound of formula (II), compound (10) in which R1 is H, PI is methylene, P2 is methylene, Q is methylene, Z is sulphur, Y is 4-methylpentyl, (X)o is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:- (3a7?,7a/?)4-(2S-Benzyloxy-4-methylpentanoyl)hexahydrotiiieno[3,2-fe]pyridin-3-one A third example compound of formula (II), compound (11) in which R1 is H, P! is methylene, P2 is methylene, Q is methylene, Z is methylene, Y is 4-methylpentyl, (X)0 is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:- (4aS,7aS) l-(25-Benzyloxy-4-methylpentanoyl)octahydrocyclopenta[6]pyridin-7-one A fourth example compound of formula (II), compound (12) in which R1 is H, PI is methylene, Pa is methylene, Q is methylene, Z is NH, Y is 4-methylpentyl, (X)0 is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:- (3a5,7a^)4-(21S-Ben2yloxy-4-methylpentanoyl)octahydropyrrolo[3,2-i]pyridin-3-one A fifth example compound of formula (II), compound (13) in which R1 is H, PI is methylene, P2 is methylene, Q is NH, Z is oxygen, Y is 4-methylpentyl, (X)o is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:- (4a/?,7a5)4-(25-Benzyloxy-4-methylpentanoyl)hexahydrofuro[3,2-c]pyridazin-7-one A sixth example compound of formula (II), compound (14) in which R1 is H, PI is methylene, P2 is methylene, Q is NH, Z is sulphur, Y is 4-methylpentyl, (X)o is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:- (4a^,7a/?)4-(21S-Benzyloxy-4-methylpentanoyl)hexahydrofuro[3,2-c]pyridazin-7- one A seventh example compound of formula (II), compound (15) in which R1 is H, PI is methylene, P2 is methylene, Q is NH, Z is methylene, Y is 4-methylpentyl, (X)o is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:- -(25-Ben2yloxy-4-methylpentanoyl)octahydrocyclopenta[c]pyridazin-7- one An eighth example compound of formula (II), compound (16) in which R1 is H, PI is methylene, Pa is methylene, Q is NH, Z is NH, Y is 4-methylpentyl, (X)0 is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:- 4-(21Sf-Benzyloxy-4-methylpentanoyl)octahydropyrrolo[3,2-c]pyridazhi-7- one Compounds of the invention include, but are not limited to, the following examples that are the (3aS, 6aR) isomer of general formula (I), where Z = 'O' and R1 = 'H', and also include the equivalent analogues included in the full definition of Z and R1 7V-[l-(4-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydroiuro[3,2-6]pyrrol-4-yl)ethyl] benzamide 4-/ert-Butyl-JV-[l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofiiro[3,2-Z)]pyrrol-4-yl)ethyl]benzamide Ar-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]-4-trifluoromethoxyb enzamide 4-Dimethylamino-7V'-[l-(4-liydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[332-6]pyrrol-4-yl)ethyl]benzamide 7Vr-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydroftiro[3,2-i]pyrrol-4-yl)ethyl]-4-isopropylbenzamide 4-Difluoromethoxy-7V-[l-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fc]pyrrol-4-yl)etiiyl]benzamide 7V-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyirol-4-yl)ethyl]-4-trifluoromethylbenzamide 4-Bromo-JV-[l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide 3-Bromo-JV-[l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-liexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide jV-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-^]pyrrol-4-yl)ethyl]-4-vinylbenzamide Naphthalene-2-carboxylic acid [l-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-fe]pyrrol-4-yl)ethyl]amide Naphthalene-1-carboxylic acid [l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3 52-6]pyrrol-4-yl)ethyl]amide Quinoline-6-carboxylic acid [1 -(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-fc]pyrrol-4-yl)ethyl]amide Benzo[b]thiophene-2-carboxylic acid [1 -(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-&]pyrrol-4-yl)ethyl]amide Benzo[6]thiophene-3-carboxylic acid [l-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-fe]pyrrol-4-yl)ethyl]amide Benzothiazole-5-carboxylic acid [l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-b.exahydro furo[3,2-6]pyrrol-4-yl)ethyl]amide Biphenyl-4-carboxylic acid [ 1 -(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]amide /A[l-(4-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-phenoxybenzamide AT-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-fe]pyrrol-4-yl)ethyl]-3-phenoxybenzamide N-[l -(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]-4-imidazol-1 -ylbenzamide //-[l-(4-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-^]pyrrol-4-yl)ethyl]-4-thiopb.en-2-ylbenzamide JV-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydroftiro[3,2-&]pyrrol-4-yl)ethyl]-4-oxazol-5 -ylbenzamide jV"-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-[1,2,3]thiadiazol-5-ylbenzamide .V-[l.(4.Hydroxybeiizyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-pyrazol-1 -ylbenzamide N-[l-(4-Hydroxybeiizyl)-2-oxo-2-(3-oxo-liexahydrofuro[3,2-fe]pyrrol-4-yl)ethyl]-5-tMopb.en-2-ylnicotmainide Ar-[l 2-Phenylthiazole-4-carboxylic acid [l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa b.ydrofuro[3,2-6]pyrrol-4-yl)ethyl]ainide 2-Pyridin-3-ylthiazole-4-carboxylic acid [l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide 5-Pb.enyltMophaie-2-carboxylic acid [1 -(4-hydroxybenzyl)-2-oxo-2-(3-oxo-liexa hydrofuro[3,2-fe]pyrrol-4-yl)ethyl]amide 5-Pyridin-3-ylthiophene-2-carboxylic acid [l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide 2-Methyl-5-phenylfuran-3-carboxylic acid [1 -(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexab.ydrofuro[352-fc]pyrrol-4-yl)ethyl]amide 4-Phenylthiophene-2-carboxylic acid [1 -(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa b.ydrofuro[3,2-6]pyrrol-4-yl)etiiyl]amide 4-Pyridin-3-yltbiophene-2-carboxylic acid [l-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fc]pyrrol-4-yl)ethyl]amide 2-Tbiophen-2-ylthiazole-4-carboxylic acid [1 -(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]amide 2-Methyl-5-(pyrrolidine-l-sulfonyl)furan-3-carboxylic acid [l-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide 3-Phenylpyrrole-l-carboxylic acid [l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-^]pyrrol-4-yl)ethyl]amide ^-[3-Meth.yl-l-(3-oxo-hexahydrofuro[3,2-t]pyrrole-4-carbonyl)butyl]beo2ainide benzamide JV-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-fe]pyrrole-4-caibonyl) butyl]-4-trifluoro methoxybenzamide 4-Dime%lamino-JV'-[3-meihyl-l-(3-oxo-hexahydrofuro[3,2-fe]pyrrole-4-carbonyl) butyl]benzamide 4-Isopropyl-^-[3-methyl-l-(3-oxo-hexahydrofuro[3,2-i]pyrrole-4-carbonyl) butyl] benzamide 4-Difluoromethoxy-7V'-[3-methyl-l-(3-oxo-hexab.ydrofuro[3,2-fe]pyrrole-4-carbonyl) butyl]benzamide ^T-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-&]pyrrole-4-cafbonyl) butyl]-4-trifluoro methylbenzamide 4-Bromo-^-[3-methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl] benzamide 3-Bromo-N-[3-methyl-1 -(3-oxo-hexahydn3furo[3,2-6]pyrrole-4-carbonyl) butyl] benzamide ^-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-Zj]pyrrole-4-carbonyl) butyl]-4-vinyl benzamide Naphthalene-2-carboxylic acid [3 -methyl-1 -(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl] amide Naphthalene-1-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-Z)]pyrrole-4-carbonyl) butyl]amide Quinoline-6-cafboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]amide Benzo[&]thiophene-2-carboxylic acid [3-methyl-l -(3-oxo-hexahydrofuro[3,2-&] pyrrole-4-carbonyl) butyl]amide Benzo[&]thiophene-3-carboxylic acid [3-methyl-l -(3-oxo-hexahydrofuro[3,2-&] pyrrole-4-carbonyl) butyl]amide Benzothiazole-5-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl] amide Biphenyl-4-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) buryl]amide 7^-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]-4-phenoxy benzamide 7V-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]-3-phenoxy benzamide 4-Imidazol-l-yl-AT-[3-methyl-l-(3-oxo-liexahydrofuro[3}2-t]pyrrole-4-carbonyl) butyljbenzamide Ar-[3-Methyl-l-(3-oxo-hexahydrofiiro[352-6]pyrrole-4-carbonyl) butylj-4-thiophen-2-ylbenzamide JV-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]-4-oxazol-5-ylbenzamide ]V-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]-4-[ 1,2,3] thiadiazol-4-ylbenzamide 7V-[3-Methyl-l-(3-oxo-liexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]-4-pyrazol-l-ylbenzamide 7sT-[3-Methyl-l-(3-oxo-hexaliydrofuro[3}2-6]pyrrole-4-carbonyl) butyl]-5-tbiophen-2-ylnicotinamide JV-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyTrole-4-carbonyl) butylj-6-phenyl nicotinamide 2-Phenylthiazole-4-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-6] pyrrole-4-carbonyl) butyl]amide 2-Pyridin-3-ylthiazole-4-carboxylicacid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-Z?]pyrrole-4-cafbonyl) butyl]amide 5-Phenylthiophene-2-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-&] pyrrole-4-carbonyl) butyl]amide 5-Pyridm-3-ylthiophene-2-carboxylic acid [3-metiiyl-l -(3-oxo-hexahydrofuro[3,2-6] pyrrole-4-carbonyl) butyl]amide 2-Methyl-5-phenylfuran-3-carboxylic acid [3-memyl-l-(3-oxo-hexahydrofuro[3,2-6] pyrrole-4-carbonyl) butyl]amide 4-Phenylthiophene-2-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-6] pyrrole-4-carbonyl) butyl] amide 4-Pyridin-3-ylthiophene-2-carboxylic acid [3-methyl-1 -(3-oxo-hexahydrofuro [3,2-b] pyrrole-4-carbonyl) butyl]amide 2-Thiopb.en-2-ylthiazole-4-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3s2-^] pyrrole-4-carbonyl) butyljamide 2-Methyl-5-(pyn-oUdine-l-sulfonyl)furan-3-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-6] pyrrole-4-carbonyl) butyl]amide 3-?henylpyrrole-l-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyrrole -4-carbonyl) butyljamide ^-[l-(3-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl] benzamide 4-fe7t-Butyl-A^[l-(3-hydroxybenzyl)-2-oxo-2-(3-oxo-liexahydroruro[3,2-i]pyrrol-4-yl)ethyl]benzamide Ar-[l-(3-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[352-6]pyrrol-4-yl)ethyl]-4-trifluoromethoxybenzamide 4-Dime%lamino-//-[l-(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-£]pyrrol-4-yl)ethyl]benzamide ^V-[l-(3-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-^]pyrrol-4-yl)ethyl]-4-isopropylbenzamide 4-Difluoromethoxy-JV-[l-(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide AT-[l-(3-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]-4-trifluoromethylbenzamide 4-Bromo-JV'-[l-(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-Z?]pyrrol-4-yl)ethyl]benzamide 3-Bromo-Ar-[l-(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide 2V-[l-(3-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofiaro[3,2-6]pyrrol-4-yl)ethyl]-4-vinylbenzamide Naphthalene-2-carboxylic acid [1 -(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-fe]pyTrol-4-yl)ethyl]amide Naphthalene- 1-carboxylic acid [l-(3-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofiiro [3 ^2-6]pyrrol-4-yl)ethyl]amide Quinoline-6-carboxylic acid [1 -(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]amide Benzo[6]thiophene-2-carboxylic acid [1 -(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa hydrofiiro[3,2-Z)]pyrrol-4-yl)ethyl]amide Benzo[6]thiophene-3-carboxylic acid [ 1 -(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro [3,2-6]pyirol-4-yl)ethyl]amide Benzothiazole-5-carboxylic acid [1 -(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydro fiiro[3,2-fe]pyrrol-4-yl)ethyl]amide Biphenyl-4-carboxylic acid [l-(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-i]pyrrol-4-yl)ethyl]amide JV-[l-(3-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-phenoxybenzamide AT-[l-(3-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-i]pyrrol-4-yl)ethyl]-3-phenoxybenzamide ^V-Cl-(3-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-63pyrrol-4-yl)ethyl]-4-imidazol-1 -ylbenzamide 7/-[l-(3-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-thiophen-2-ylbenzainide A^-[l Ar-[l-(3-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fc]pyrrol-4-yl)ethyl]-4-[ 1 }2,3]thiadiazol-5-ylbenzamide yV-[l-(3-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]-4-pyrazol-1 -ylb enzamide A^[l-(3-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)et;hyl]-5-thiophen-2-ylnicotinamide A^-[l-(3-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-6-phenylnicotinamide 2-Phenylthiazole-4-carboxylic acid [1 -(3-hydroxyben2yl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-&]pyrrol-4-yl)ethyl]amide 2-Pyridin-3-ylthiazole-4-carboxylic acid [l-(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide 5-Phenylthiophene-2-carboxylic acid [ 1 -(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-Z>]pyrrol-4-yl)ethyl]amide 5-Pyridin-3-yltbiophene-2-carboxylic acid [1 -(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide 2-Methyl-5-phenylfuran-3-cafboxylic acid [1 -(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)eihyl]amide 4-Phenylthiophene-2-carboxylic acid [1 -(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide 4-Pyridin-3-ylthiophene-2-carboxylic acid [l-(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydroforo[3,2-6]pyrrol-4-yl)etliyl]amide 2-Thiophen-2-ylthiazole-4-carboxylic acid [ 1 -(3-hydroxybenzyl)-2-oxo-2-(3 -oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide 2-Methyl-5-(pyrrolidine-l-sulfonyl)furan-3-carboxylic acid [l-(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide 3-Phenylpyrrole-l-carboxylic acid [l-(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydro iuro[3,2-6]pyrrol-4-yl)ethyl] amide Ar-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-4-ylmethylethyl] benzamide 4-/ferr-Butyl-JV-[2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]benzamide 7^-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]-4-trifluoromethoxybenzamide 4-Dimethylamino-JV-[2-oxo-2-(3-oxo-hexahydrofiu>o[3,2-&]pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]benzamide ^-[2-Oxo-2-(3-oxo-hexahydrofco[3,2-6]pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]-4-isopropylbenzamide 4-Difluoromethoxy-/^-[2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]benzamide A^[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)-l-pyridin-4-ylmetliylet:hyl]-4-trifluoromethylbenzamide 4-Bromo-Ar-[2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]benzamide 3-Bromo-//-[2-oxo-2--(3-oxo-hexahydroftiro[3)2-6]pyrrol-4-yl)-l-pyridin-4-yl methylethyljbenzamide A7-[2-Oxo-2-(3-oxo-hexahydrofuro[332-6]pyrrol-4-yl)-l-pyridin-4-ylmet;hylethyl]-4-\dnylbenzamide Naphthalene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]amide Naphthalene-1-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-4-yknethylethyl]amide Quinoline-6-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[352-£]pyrrol-4-yl)-l -pyridin-4-ylmethylethyl]amide Benzo[6]thiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-1 -pyridin-4-yhnethylethyl] amide Benzo[fe]thiophene-3-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3^-6]pyrrol-4-yl)-1 -pyridin-4-yhnethylethyl]amide Benzotniazole-5-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-1 -pyridin-4-ylinethylethyl]amide Biphenyl-4-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3 s2-i>]pyrrol-4-yl)-1 -pyridin-4-ylmethylethyl]amide AT-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-4-yhnethylethyl]-4-phenoxybenzamide Ar-[2-Oxo-2-(3-oxo-hexahydrofoo[3,2-fe]pyrrol-4-yl)-l-pyridin-4-ylinethyletliyl]-3-phenoxybenzamide [3,2-fe]pyrrol-4-yl)-l-pyridin-4-ylmetliylethyl]-4-imidazol-1 -ylbenzamide Ar-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-i]pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]-4-thiophen-2-ylbenzamide A^-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-fc]pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]-4-oxazol-5-ylbenzamide jV-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyiTol-4-yl)-l-pyridin-4-ylinethyleihyl]-4-[ 1,2,3]thiadiazol-5-ylbenzainide Ar-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrroM-yl)-l-pyridin-4-ylmethylethyl]-4-pyrazol-1 -ylbenzamide Ar-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-1 -pyridm-4-ylmethylethyl j-5-thiophen-2-ylnicotinamide AT-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]py]Tol-4-yl)-l-pyridin-4-ylmel3iyletiiyl]-6-phenylnicotinamide 2-Phenylthiazole-4-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-1 -pyridin-4-ylmethylethyl]amide 2-Pyridin-3-ylthiazole-4-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6] pyrrol-4-yl)-l-pyridin-4-ylmetb.ylethyl]amide 5-Phenylthiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-i]pyrrol-4-yl)-1 -pyridin-4-ylmethylethyl]amide 5-Pyridin-3-ylthiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]amide 2-Methyl-5-phenylfuran-3-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe] pyrrol-4-yl)- 1 -pyridin-4-ylmethylethyl] amide 4-Phenylthiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l -pyridin-4-ylmethylethyl]amide 4-Pyridin-3-ylthiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-&] pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]amide 2-Thiophen-2-ylthiazole-4-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofiiro[3,2-fc] pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]amide 2-Methyl-5-(pyrrolidine-l-sulfonyl)furan-3-carboxylic acid [2-oxo-2-(3-oxo-b.exa hydrofuro[3,2-i]pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]arnide 3-Phenylpyrrole-l -carboxylic acid [2-oxo-2-(3-oxo-liexahydrofuro[3,2-&]pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]amide benzamide 4-te^Butyl-^-[2-oxo-2 trifluoTomethoxybenzamide 4-Dimethylamino-^-[2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-3-ylmethylethyl]benzamide A^-[2-Oxo-2-(3-oxo-hexahydrofuro[352-i]pyrrol-4-yl)-l-pyridin-3-ylmethylethyl]-4-isopropylbenzamide 4-Difluoromethoxy-A^[2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-3-ylmethylethyl]benzamide A^-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)-l-pyridin-3-yhnethylethyl]-4-trifluoromethylbenzainide 4-Bromo-JV-[2-oxo-2-(3 -oxo-hexahy drofuro[3,2-6]pyrrol-4-yl)-1 -pyridin-3-yhnethylethyl]benzamide 3-Bromo-7V-[2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)-l-pyridin-3-yhnethylethyljbenzamide JV"-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-Z?]pyrrol-4-yl)-l-pyridin-3-yknethylethyl]-4-vinylbenzamide Naphthalene-2-cafboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)-l-pyridm-3-ylmethylethyl]amide Naphthalene-1-carboxyUc acid [2-oxo-2-(3-oxo-hexahydroiuro[3J2-6]pyrrol-4-yl)-l-pyridin-3-yhnethylethyl]amide Quinoline-6-cafboxylic acid [2-oxo-2-(3 -oxo-hexahydrofuro [3,2-Z»]pyrrol-4-yl)-1 -pyridin-3-ylmethylethyl]amide Benzo[6]thiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofwo[3,2-6]pyrrol-4-yl)-l -pyridin-3-yknethylethyl]amide Benzo[6]tbiophene-3-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol--pyridin-3-yhnefhylethyl]amide Benzothia2ole-5-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3s2-6]pyrrol-4-yl)-1 -pyridin-3-ylmethylethyl]amide Biphenyl-4-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofviro[3,2-6]pyrrol-4-yl)-l -pyridin-3 -ylmethylethyljamide A^-[2-Oxo-2-(3-oxo-hexahydrofuro[3J2-6]pyrrol-4-yl)-l-pyridin-3-ylmethylethyl]-4-phenoxybenzamide Ar-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-3-ylmethyletliyl]-3-phenoxybenzamide W-[2-Oxo-2-(3-oxo4iexahydrofuro[3,2-&]pyrroM imidazol-1 -ylbenzamide JV"-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyiroW-yl)4-pyridin-3-ylmethylethyl]-4-thiophen-2-ylbenzamide 7^[2-Oxo-2-(3-oxo-hexahydroforo[3>6]pyrroW-yl)-l-pyridin-3-ylmethylethyl]-4-oxazol-5-ylbenzamide 7vr-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-3-ylmethylethyl]-4-[ 1,2,3]thiadiazol-5-ylbenzamide Ar-[2-Oxo-2-(3-oxo-hexahydrofiiro[3,2-6]pyrrol-4-yl)-l-pyridia-3-ylniethylethyl]-4-pyrazol-1 -ylbenzamide A'-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-3-ylmethylethyl]-5-tbiophen-2-ylnicotinamide 7\/-[2-Oxo-2-(3-oxo-hexahy(kofuro[3,2-i]pyrrol-4-yl)-l-pyridin-3-ylmethylet:hyl]-6-phenylnicotinamide 2-Phenylthiazole-4-carboxylic acid [2-oxo-2-(3-oxo-hexahydroforo[3 ,2-&]pyrrol-4-yl)- 1 -pyridin-3 -ylmethylethyl]amide 2-Pyridin-3-ylthiazole-4-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6] pyrrol-4-yl)-l-pyridin-3-ylmethylethyl]amide 5-Phenylthiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)- 1 -pyridin-3 -ylmethylethyl] amide 5-Pyridin-3-ylthiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-)- 1 -pyridin-3-ylmethylethyl]amide 2-Methyl-5-phenylfuran-3-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-&] pyrrol-4-yl)-l-pyridin-3-ylmethylethyl]amide 4-Pb.enylthiophene-2-cafboxylic acid [2-oxo-2-(3-oxo-hexah.ydrofuro[3,2-6]pyrrol-4-yl)- 1 -pyridin-3-ylmethylefliyl]amide 4-Pyridin-3-ylthiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6] pyrrol-4-yl)- 1 -pyridin-3-ylmethylethyl]amide 2-Thiophen-2-ylthiazole-4-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6] pyrrol-4-yl)-l-pyridin-3-ybiiethyletliyl]amide 2-Methyl-5-(pyrrolidine-l-sulfonyl)furan-3-carboxylic acid [2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-3-yknethylethyl]amide 3-Phenylpyrrole-l-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)-l -pyridin-3-ylmethylethyl] amide A'.[l_(4-Bromobenzyl)-2-oxo-2-(3-oxo-hexaiiydrofuro[3,2-6]pyrrol-4-yl)ethyl] benzamide 4-re7^-Butyl-JV-[l-(4-bromoben2yl)-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-&]pyrrol-4-y 1) ethyl]benzamide Ar.[ 1 .(4-Bromobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]-4-trifluoromethoxybenzamide 4-Dime1hylamino-Ar.[l-(4-broinobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2--Z?]pyrrol-4-yl)ethyl]benzamide A-[l-(4-Bromobenzyl)-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-&]pyrrol-4-yl)ethyl]-4-isopropylbenzamide 4-Difluoromethoxy-JV'-[l-(4-bromobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide ^-[l-(4-Bromobenzyl)-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-fc]pyirol-4-yl)ethyl]-4-trifluoromethylbenzamide 4-Bromo-Ar-[l-(4-bromoben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[352-6]pyrrol-4-yl)ethyl]benzamide 3-Bromo-7V'-[l-(4-bromoben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)ethyl]benzamide Ar-[l-(4-Bromobenzyl)-2-oxo-2-(3-oxo-liexaliydrofuro[3,2-&]pyrrol-4-yl)ethyl]-4-vinylbenzamide Naphthalene-2-carboxylic acid [1 -(4-bromobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-fe]pyrrol-4-yl)ethyl]amide Naphthalene-1 -cafboxylic acid [l-(4-bromobenzyl)-2-oxo-2-(3-oxo-hexahydrof\iro [3,2-6]pyrrol-4-yl)ethyl]amide Quinoline-6-carboxylic acid [ 1 -(4-bromobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-&]pyrrol-4-yl)ethyl]aimde Benzo[6]thiophene-2-carboxylic acid [l-(4-bromobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-&]pyrrol-4-yl)ethyl]amide Benzo[6]thiophene-3-carboxylic acid [1 -(4-bromobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-£>]pyrrol-4-yl)ethyl]amide Benzotbiazole-5-carboxylic acid [l-(4-bromoben2yl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-^]pyrrol-4-yl)ethyl]amide Bipb.enyl-4-carboxylic acid [l-(4-bromoben2yl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-fe]pyrrol-4-yl)ethyl]ainide ^-[l-(4-Bromoben2yl)-2-oxo-2 A^-[l-(4-Bromobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fc]pyrrol-4-yl)ethyl]-3-phenoxybenzamide A/"-[l-(4-Bromobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]-4-imidazol-1-ylbenzamide 7V-[l-(4-Bromobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3J2-6]pyrrol-4-yl)ethyl]-4-thiophen-2-ylberizamide N-[l-(4-Bromobenzyl)-2-oxo-2-(3-oxo-hexahydroftiro[3,2-6]pyrrol-4-yl)ethyl]-4-oxazol-5 -ylbenzamide 7^-[l-(4-Bromoben2yl)-2-oxo-2-(3-oxo-liexahydrofuro[3,2-fe]pyrrol-4-yl)ethyl]-4-[1,2,3]thiadiazol-5-ylbenzainide vV-[l-(4-Bromobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]-4-pyrazol-1 -ylbenzamide Ar-[l-(4-Bromobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-5-thiophen-2-ylnicotinamide N-[ 1 -(4-Bromoben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-6-phenylnicotinamide 2-Phenylthiazole-4-carboxylic acid [l-(4-bromobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-£>]pyrrol-4-yl)ethyl]amide 2-Pyridin-3-ylthia2ole-4-carboxylic acid [l-(4-bromobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-i]pyiTol-4-yl)ethyl]anude 5-Phenylthiophene-2-carboxylic acid [l-(4-bromobenzyl)-2-oxo-2-(3-oxo-liexa hydrofuro[332-fe]pyrrol-4-yl)ethyl]ainide 5-Pyridin-3-ylthiopheae-2-carboxylic acid [1 -(4-bromobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-Z)]pyrrol-4-yl)ethyl]aniide 2-Methyl-5-plienylfuran-3-carboxylic acid [1 -(4-bromobenzyl)-2-oxo-2-(3-oxo-hexah.ydrofuro[3,2-fe]pyrrol-4-yl)ethyl]amide 4-PhenyIthiophene-2-carboxyIic acid [1 -(4-bromobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide 4-Pyridin-3-ylthiophene-2-carboxylic acid [1 -(4-bromobenzyl)-2-oxo-2-(3-oxo-hexahydroftiro[3,2-fr]pyrrol-4-yl)ethyl]ainide 2-Thiophen-2 -ylthiazole-4-carboxylic acid [l-(4-bromobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]amide 2-Methyl-5-(pyrrolidine-l-sulfonyl)furan-3-carboxylic acid [l-(4-bromoben2yl)-2-oxo-2-(3-oxo-hexahydroftiro[3,2-i]pyrrol-4-yl)ethyl]amide 3-Phenylpyrrole-l-carboxylic acid [1 -(4-bromobenzyl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-6]pyrrol-4-yl)ethyl]amide Ar-[l-(4-Fluorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)eihyl] benzamide 4-fer^Butyl-7/-[l-(4-fluoroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide Ar-[l-(4-Fluoroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3J2-6]pyrrol-4-yl)ethyl]-4-trifluoromethoxybenzamide 4-Dimethylamino-JV-[l-(4-fluorobenzyl)-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-i]pyrrol-4-yl)ethyl]benzamide 7/-[l-(4-Fliiorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)et;hyl]-4-isopropylbenzamide 4-Difluoromethoxy-7V-[l-(4-fluorobenzyl)-2-oxo-2-(3-oxo-liexaliydrofuro[3,2-£j]pyrrol-4-yl)ethyl]benzamide Ar-[l-(4-Fluoroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-trifluoromethylbenzamide 4-Bromo-Ar-[l-(4-fluoroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-Z?]pyrrol-4-yl)ethyl]benzamide 3-Bromo-A;'-[l-(4-fluorobeii2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3s2-6]pyrrol-4-yl)ethyl]benzamide A^[l-(4-Fluorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)eihyl]-4-\inylbenzamide "Naphthalene-2-carboxylic acid [1 -(4-fluoroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]amide Naphthalene-1-carboxylic acid [l-(4-fluoroben2yl)-2-oxo-2-(3-oxo-hexah.ydrofuro [3,2-fe]pyrrol-4-yl)ethyl]amide Quinoline-6-carboxylic acid [l-(4-fluorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-i]pyrrol-4-yl)ethyl] amide Benzo[6]thiophene-2-carboxylic acid [l-(4-fluorobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)ethyl]aniide Benzo[fe]thiophene-3-carboxylic acid [l-(4-fluorobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide Benzothiazole-5-carboxylic acid [l-(4-fluorobenzyl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-fe]pyrrol-4-yl)ethyl]amide Biphenyl-4-carboxylic acid [l-(4-fluoroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]amide . ^/•.[l.(4_FlUorobenzyl)-2-oxo-2-(3-oxo-hexahydroiuro[3,2-Z)]pyrrol-4-yl)ethyl]-4-phenoxybenzamide AT-[l-(4-Fluorobenzyl)-2-oxo-2-(3-oxo-hexabydrofuro[352-fc]pyrrol-4-yl)ethyl]-3-phenoxybenzamide 7^-[l-(4-Fluorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-b]pyrrol-4-yl)et:hyl]-4-imidazol-1 -ylbenzamide JV-[l-(4-Fluorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-thiophen-2-ylben2amide //-[l-(4-Fluorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3J2-6]pyrrol-4-yl)ethyl]-4-oxazol-5-ylbenzamide ^-[l 7/-[l-(4-Fluoroben2yl)-2-oxo-2-(3-oxo-hexahydroiuro[3,2-fe]pyrrol-4-yl)ethyl]-4-pyrazol-1 -ylbenzamide JV-[l-(4-Fluoroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3J2-6]pyrrol-4-yl)eihyl]-5-tbiophen-2-ylnicotinainide JV-[l-(4-Fluorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]-6-phenylnicotinamide 2-Phenylthiazole-4-carboxylic acid [1 -(4-fluorobenzyl)-2-oxo-2-(3-oxo-hexa hydrofiiro[3,2-6]pyrrol-4-yl)ethyl]amide 2-Pyridin-3-ylthiazole-4-carboxylic acid [1 -(4-fluorobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide 5-Phenylthiophene-2-carboxylic acid [l-(4-fl.uorobenzyl)-2-oxo-2-(3-oxo-liexa hydrofuro[3,2-6]pyrrol-4-yl)ethyl]atnide 5-Pyridin-3-yliMophene-2-carboxylic acid [l-(4-fluorobenzyl)-2-oxo-2-(3-oxo-hexahydroforo[3,2-&]pyrrol-4-yl)ethyl]amide 2-Methyl-5-phenylfuran-3-carboxylic acid [l-(4-fluorobenzyl)-2-oxo-2-(3-oxo-hexahydrofi3ro[3,2-6]pyrrol-4-yl)et:hyl]amide 4-Phe«yltbiophene-2-carboxylic acid [l-(4-fluoroben2yl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-i]pyrrol-4-yl)ethyl]amide 4-Pyridin-3-ylthiophene-2-cafboxylic acid [l-(4-fluorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)ethyl]amide 2-Tbiophen-2-ylthiazole-4-cafboxylic acid [l-(4-fiuoroben2yl)-2-oxo-2-(3-oxo-b.exahydrofuro[3,2-Z)]pyrrol-4-yl)eihyl]amide 2-Methyl-5-(pyrrolidine-l-sulfonyl)furan-3-carboxylic acid [l-(4-fluorobenzyl)-2-oxo-2-(3-oxo-b.exahydrof\aro[3}2-6]pyrrol-4-yl)ethyl]amide 3-Phenylpyrrole-l-carboxylic acid [l-(4-fluorobenzyl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-fe]pyrrol-4-yl)ethyl]amide jV-[l -(4-Chlorobenzyl)-2-oxo-2-(3 -oxo-hexahydroforo[3,2-6]pyrrol-4-yl)ethyl] benzamide yl)ethyl]benzamide JV-[l-(4-Chlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-trifluoromethoxybenzamide 4-Dimethylamino-JV-[l-(4-chloroben2yl)-2-oxo-2-(3-oxo-hexaliydrojEuro[3,2-6]pyrrol-4-yl)ethyl]benzamide ^-[l-(4-Chlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofiiro[3,2-&]pyrrol-4-yl)ethyl]-4-isopropylbenzamide 4-Difluoromethoxy-JV-[l-(4-chlorobenzyl)-2-oxo-2-(3-oxo-hexaliydrofuro[332-i]pyrrol-4-yl)ethyl]benzamide 7^-[l-(4-CMoroben2yl)-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-%yrrol-4-yl)ethyl]-4-trifluoroinethylbenzaniide 4-Bromo-7/-[l-(4-chlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide 3-Bromo-JV"-[l-(4-cbloroben2yl)-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-fe]pyrrol-4-yl)ethyl]benzaniide 7^-[l-(4-Chloroberi2yl)-2-oxo-2-(3-oxo-hexahydrofuro[332-Z)]pyrrol-4-yl)ethyl]-4-vinylbenzaniide Naphthalene-2-carboxylic acid [ 1 -(4-chloroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]amide Naphthalene-1-cafboxylic acid [l-(4-chlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]amide Quinoline-6-cafboxylic acid [1 -(4-chlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3 ,2-6]pyrrol-4-yl)ethyl]amide Benzo[6]thiophene-2-carboxylic acid [l-(4-chlorobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-&]pyrrol-4-yl)etbyl]amide Benzo[6]thiophene-3-carboxylic acid [1 -(4-chloroben2yl)-2-oxo-2-(3-oxo-hexa hydro furo[3,2-6]pyrrol-4-yl)ethyl]amide Benzothiazole-5-carboxylic acid [1 -(4-chlorobenzyl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-6]pyrrol-4-yl)ethyl]amide Biphenyl-4-carboxylic acid [l-(4-chloroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro 6]pyrrol-4-yl)eihyl]aniide AT-[l-(4-CUorobenzyl)-2-oxo-2-(3-oxo-hexahydroftu:o[3,2-fe]pyrrol-4-yl)ethyl]-4-phenoxybenzamide JV"-[l-(4-(^orobeii2yl)-2-oxo-2-(3-oxo-hexahydroftiro[3,2-fc]pyiToW-yl)ethyl]-^ phenoxybenzamide A^[l-(4-CWoroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-imidazol- 1 -ylbenzamide 7V-[l-(4rChlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl3-4-thiophen-2-ylbenzamide ^-[l-(4-Chloroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-oxazol-5-ylbenzamide _?V.[l.(4-Chlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-[1,2,3]thiadiazol-5-ylbenzainide A^-[l-(4-Chlorobenzyl)-2-oxo-2-(3-oxo-hexahydroftiro[3,2-6]pyrrol-4-yl)ethyl]-4-pyrazol-1 -ylbenzamide AT.[i_(4.chlorobenzyl)-2-oxo-2-(3-oxo-hexaliydroforo[3,2-6]pyrrol-4-yl)ethyl]-5-thiophen-2-ylnicotinamide A^[l-(4-Chlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-6-phenylnicotinamide 2-Phenylthiazole-4-carboxylic acid [1 -(4-chlorobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-fc]pyrrol-4-yl)etb.yl]amide 2-Pyridin-3-ylthiazole-4-cai-boxylic acid [ 1 -(4-chlorobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-fe]pyrrol-4-yl)ethyl]amide 5-Phenyltbiophene-2-carboxylic acid [ 1 -(4-chlorobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide 5-Pyridin-3-ylthiopliene-2-carboxylic acid [l-(4-cblorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-i]pyrrol-4-yl)ethyl]amide 2-Methyl-5-phenylfuran-3-carboxylic acid [l-(4-chloroben2yl)-2-oxo-2-(3-oxo-b.exahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide 4-Phenyltbiophene-2-carboxylic acid [1 -(4-chloroben2yl)-2-oxo-2-(3-oxo-hexa hydrofuro[352-6]pyirol-4-yl)ethyl]arnide 4-Pyridin-3-yltbiophene-2-carboxylic acid [1 -(4-chlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-i]pyrrol-4-yl)ethyl]amide i-Thiophen-2-ylthiazole-4-carboxylic acid [1 -(4-chlorobenzyl)-2-oxo-2-(3-oxo-iexahydrofuro[3,2-6]pyrrol-4-yl)eihyl]amide 2-Methyl-5-(pyrrolidiae-l -sulfonyl)furan-3-carboxylic acid [ 1 -(4-chlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)ethyl]amide 3-Phenylpyrrole-1 -carboxylic acid [1 -(4-chloroben2yl)-2-oxo-2-(3 -oxo-hexahydro furo[3,2-6]pyrrol-4-yl)ethyl]amide 7^-[l-(3,4-DicUorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[352-Z)]pyrrol-4-yl)ethyl] benzamide 4-fert-Butyl-^[l-(3J4-dichlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3J2-fc]pyrrol-4-yl)ethyl]benzamide Ar-[l-(3,4-Dichloroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)eihyl]-4-trifl.uoromethoxybenzamide 4-Dimeihylamino-N'-[l-(3s4-dicUoroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide JV-[l-(3,4-Dichloroben2yl)-2-oxo-2-(3-oxo-liexaliydrofliro[3,2-i]pyrrol-4-yl)ethyl]-4-isopropylbenzamide 4-Difluoromeflioxy-JV-[l-(3,4-dichlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide ^-[l-(3,4-DicMoroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyirol-4-yl)ethyl]-4-trifluoromethylben2amide 4-Bromo-W-[l-(3,4-dichlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide 3-Bromo-7\T-[l-(3,4-diclilorobenzyl)-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide ^.[i_(3)4-Dichlorobenzyl)-2-oxo-2-(3-oxo-liexahydrofuro[3)2-6]pyrrol-4-yl)ethyl]-4-vinylbenzamide Naphthalene-2-carboxylic acid [1 -(3,4-dichlorobenzyl)-2-oxo-2-(3-oxo- a, hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]amide Naphthalene-1-carboxylic acid [l-(354-dichlorobenzyl)-2-oxo-2-(3-oxo- hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide Quinoline-6-carboxylic acid [1 -(3,4-dichlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]amide Benzo[Z?3thiophene-2-carboxylic acid [1 -(3,4-dichlorobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-&]pyrrol-4-yl)ethyl]amide Benzo[6]thiophene-3-carboxylic acid [1 -(3,4-dicUorobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide Benzothiazole-5-carboxylic acid [l-(3,4-dichlorobenzyl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-fe]pyrrol-4-yl)ethyl]amide Biphenyl-4-carboxylic acid [1 -(3,4-dichlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [352-6]pyrrol-4-yl)ethyl]amide A^-[l-(3,4-Dichloroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-phenoxybenzamide iV-[l-(3,4-Dichlorobenzyl)-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-6]pyrrol-4-yl)ethyl]-3 -phenoxybenzamide A-[l A^[l2-6]pyrrol-4-yl)ethyl]-4-thiophen-2-ylbenzamide A:-[l-(3,4-Dichlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3J2-6]pyrrol-4-yl)ethyl]-4-oxazol-5 -ylbenzamide A'-[l-(3J4-Dichlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)efhyl]-,2,3]tbiadiazol-5-ylbenzamide Ar-[l-(3,4-Dichloroben2yl)-2-oxo-2-(3-oxo-hexahydrofurot3,2-fe]pyrrol-4-yl)ethyl]-4-pyrazol-l -ylbenzamide Ar-[l-(3J4-Dichlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofbro[3>2-6]pyrrol-4-yl)ethyl]-5-thiophen-2-ylnicotinamide Ar-[l-(3,4-Dichlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)ethyl]-6-phenylnicotinamide 2-Phenylthiazole-4-carboxylic acid [l-(3,4-dichlorobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-i]pyrrol-4-yl)ethyl]amide 2-Pyridin-3-ylthiazole-4-carboxylic acid [l-(3,4-dichlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofhro[3,2-6]pyrrol-4-yl)ethyl]amide 5-Phenylthiophene-2-carboxylic acid [l-(3,4-dichlorobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-63pyrrol-4-yl)ethyl]ainide 5-Pyridin-3-ylthiophene-2-carboxylic acid [l-(3,4-dichlorobenzyl)-2-oxo-2-(3-oxo-b.exahydrofuro[3,2-6]pyrrol-4-yl)etb.yl]amide 2-Methyl-5-phenylfuran-3-carboxylic acid [ 1 -(3,4-dichloroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide 4-Phenylthiophene-2-carboxylic acid [ 1 -(3,4-dichloroben2yl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-fe]pyrrol-4-yl)ethyl]ainide 4-Pyridin-3-yltbiophene-2-carboxylic acid [l-(3,4-dichloroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-^]pyrrol-4-yl)ethyl]ainide 2-Tbiophen-2-ylthiazole-4-carboxylic acid [ 1 -(3,4-dichlorobenzyl)-2-oxo-2-(3-oxo-b.exahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide 2-Methyl-5-(pyrrolidine-l-sulfonyl)furan-3-carboxylic acid [1 -(3,4-dichlorobenzyl)-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-£»]pyrrol-4-yl)ethyl]amide 3-PhenyIpyrrole-l-carboxylic acid [l-(3,4-dichlorobeii2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-i]pyrrol-4-yl)ethyl]amide Ar-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-thiophen-2-ylmethylethyl] benzamide 4-re7'NButyl-^-[2-oxo-2-(3-oxo-hexahydrofuro[3,2-%yirol-4-yl)-l-thiophen-2-ylmethylethyljbenzamide Ar-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-i]pyrrol-4-yl)-l-thioplien-2-yhnethylethyl]-4-trifluoroinethoxybenzainide 4-Dime%lamino-^-[2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)-l-thiophen-2-ylmethylethyl]benzamide AT-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-thiophen-2-ylmethylethyl]-4-isopropylbenzamide 4-Difluoromethoxy-^-[2-oxo-2-(3-oxo-hexahydrofuro[352-fe]pyrrol-4-yl)-l-tbiophen-2-ylmethyleihyl]benzainide Ar-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)-l-thiophen-2-ylmethylethyl]-4-trifluoromethylbenzamide 4-Bromo-7V"-[2-oxo-2-(3-oxo-hexahydrofuro[352-6]pyrrol-4-yl>l-tbiophen-2-ylmethylethyl]benzamide 3-Bromo-J\T-[2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-thiopheii-2-ylmethylethyl]benzamide Ar-[2-Oxo-2 Naphthalene-2-cafboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)-1 -thiophen-2-ylmethylethyl]amide Naphthalene-1-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)-l-thiophen-2-ybiethylethyl]ainide Quinoline-6-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)-l-thiophen-2-ylmethylethyl]amide Benzo[fc]thiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-Z?]pyrrol-4-yl)- 1 -thiophen-2-ylmethylethyl] amide Benzo[fe]thiophene-3-carboxylic acid [2-oxo-2-(3-oxo-hexahydroforo[3,2-6]pyrrol-4-yl)- 1 -thiophen-2-ylmethylethyl]amide Benzothiazole-5-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-1 -thiophen-2-ylmethylethyl]amide Biphenyl-4-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3 ,2-6]pyrrol-4-yl)- 1 -thiophen-2-ylmethylethyl]amide //-[2-Oxo-2-(3-oxo-hexahydroforo[3,2-6]pyrrol-4-yl)-l-tbiophen-2-ylmethylethyl]-4-phenoxybenzamide 3-phenoxybenzamide A^[2-Oxo-2 A^[2-Oxo-2 AT-[2-Oxo-2 ^-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)-l-thiophen-2-ylmethylethyl]-4-[ 1 ,2,3]thiadiazol-5-ylbenzamide ^-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrroI-4-yl)-l-thiophen-2-ylmethylethyl]-4-pyrazol-l -ylbenzamide Ar-[2-Oxo-2-(3-oxo-hexah.ydrofuro[3,2-fe]pyrrol-4-yl)-l-thiophen-2-ylmethyleihyl]-5-tbiophen-2-ylrucotinarnide jY-[2-Oxo-2-(3-oxo-hexahydrofuro[3J2-fe]pyrrol-4-yl)-l-thiopheri-2-ylmethylethyl]-6-phenylnicotinarnide 2-Phenylthiazole-4-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyirol-4-yl)-1 -thiophen-2-ylmethylethyl]amide 2-Pyridin-3-ylthiazole-4-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-b] pyrrol-4-yl)-1 -lMophen-2-ylmethylethyl]amide 5-PhenyltMopriene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[352-b]pyrrol-4-yl)-1 -thiophen-2-ylmethylethyl]amide 5-Pyridin-3-yltbiopb.ene-2-carboxylic acid [2-oxo-2-(3-oxo-b.exah.ydrofuro[3,2-6]pyrrol-4-yl)-1 -thiophen-2-ylmethylethyl]ainide 2-Methyl-5-phenylfuran-3-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6] pyrrol-4-yl)-l-thiophen-2-ylmeihylethyl]amide 4-Phenylthiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-1 -tbiophen-2-ylmethylethyl]amide 4-Pyridin-3-ylthiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexah.ydrofuro[3,2-b] pyrrol-4-yl)-l-thiophen-2-ylmethylethyl]amide 2-Thiophen-2-ylthiazole-4-carboxylic acid [2-oxo-2-(3-oxo-hexaliydrofuro[3,2-6] pyrrol-4-yl)-1 -thiophen-2-ylraethylethyl]amide 2-Methyl-5-(pyrrolidine-l-sulfonyl)furan-3-carboxylic acid [2-oxo-2-(3-oxo-hexa hydrofuro[3,2-Z>]pyrrol-4-yl)-l -thiophen-2-ylmethylethyl] amide 3-Phenylpyrrole-l-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-%yrrol-4-yl)-1 -thiophen-2-ylmethylethyl]amide A-[l-(4-Aminobenzyl)-2-oxo-2-(3-oxo-hexaliydrofuro[3>2-6]pyrrol-4-yl)ethyl] benzamide 4-re^Butyl-N"-[l-(4-aminobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide 7/-[l-(4-Aiiiinoben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)e^ trifluoromethoxybenzainide 4-Dimethylamino-JV"-[l-(4-aminobenzyl)-2-oxo-2-(3-oxo-b.exahydrofuro[3,2-fc]pyrrol-4-yl)ethyl]benzamide A^-[l-(4-Aminobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl3-4-isopropylbenzamide 4-Difluoromethoxy-JV-[l-(4-aminobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide ^-[l-(4-Aminobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-trifluoromeihylbenzamide 4-Bromo-^-[l-(4-aminobenzyl)-2-oxo-2-(3-oxo-hexah.ydrofuro[3,2-6]pyrrol-4-yl)ethyl]ben2;amide 3-Bromo-7V"-[l-(4-amiiiobenzyl)-2-oxo-2-(3-oxo-hexahydrofijro[3,2-fe3pyrrol-4-yl)ethyl]benzamide N-[ 1 -(4-Aminobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-vinylbenzamide Naphthalene-2-carboxylic acid [ 1 -(4-aminobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3.2-&]pyrrol-4-yl)ethyl]amide Naphthalene-1-carboxylic acid [l-(4-aminoben2yl)-2-oxo-2-(3-oxo-hexahydrofuro [3.2-fe]pyrrol-4-yl)ethyl]amide Quinoline-6-carboxylic acid [1 -(4-aminobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3.2-fc]pyrrol-4-yl)ethyl] amide Benzo[fc]tbiophene-2-carboxylic acid [l-(4-ammobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyirol-4-yl)ethyl]amide Benzo[fe]tbiophene-3-carboxylic acid [l-(4-aminobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3^2-6]pyrrol-4-yl)ethyl]amide Benzothiazole-5-carboxylic acid [l-(4-aminoben2yl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-^]pyrrol-4-yl)ethyl]amide Bipb.enyl-4-carboxylic acid [l-(4-aminobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]amide AT-[l-(4-Atninobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-phenoxybenzamide A7-[l-(4-Aminoben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]-3-phenoxybenzamide N-[ 1 -(4-Aminoben2yl)-2-oxo-2-(3 -oxo-hexahydrofuro [3,2-2>]pyrrol-4-yl)ethyl]-4-imidazol-l-ylbenzamide A'"-[l-(4-Aminobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-thiophen-2-ylbenzamide A7-[l-(4-Aminoben2yl)-2-oxo-2-(3-oxo-liexahydroftiro[3,2-Z)]pyrrol-4-yl)ethyl]-4-oxazol-5-ylbenzamide Ar-[l-(4-Aininobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-[1,2,3]thiadiazol-5-ylbenzainide Ar-[l-(4-Aminobeii2yl)-2-oxo-2-(3-oxo-hexahydrofiiro[3,2-6]pyrrol-4-yl)ethyl]-4-pyrazol-1 -ylbenzamide Ar-[l-(4-Aminoben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-5-tbiophen-2-ylnicotinamide Ar-[l-(4-Aminobeiizyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)ethyl]-6-phenylnicotinamide 2-Phenylihiazole-4-carboxylic acid [l-(4-aminobenzyl)-2-oxo-2-(3-oxo-hexa hydrofUro[3s2-Z?]pyrrol-4-yl)ethyl]amide 2-Pyridin-3-ylthiazole-4-carboxylic acid [l-(4-aminobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-63pyrrol-4-yl)ethyl]amide 5-Phenylthiophene-2-carboxylic acid [l-(4-aminobenzyl)-2-oxo-2-(3-oxo-hexa hydroiuro[3,2-2?]pyrrol-4-yl)ethyl]amide 5-Pyridin-3-ylthiophene-2-carboxylic acid [l-(4-aminoben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-i]pyrrol-4-yl)ethyl]amide 2-Methyl-5-phenylfuran-3-carboxylic acid [1 -(4-aminobenzyl)-2-oxo-2-(3-oxo-b.exahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide 4-Phenylthiophene-2-carboxylic acid [1 -(4-aminobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide 4-Pyridin-3-yltbiophene-2-carboxylic acid [1 -(4-aminobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide 2-Thiopb.en-2-ylthiazole-4-carboxylic acid [1 -(4-aminobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]atnide 2-Methyl-5-(pyrrolidine-l-sulfonyl)furan-3-carboxylic acid [l-(4-aminoben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fc]pyrrol-4-yl)ethyl]amide 3-Phenylpyrrole-l-carboxylic acid [l-(4-aminobenzyl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-6]pytrol-4-yl)ethyl]amide N-[l -Benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fc]pyrrol-4-yl)ethyl] benzamide 4-fe^^Butyl-A^-[l.ben2yl-2-oxo-2-(3-oxo-l^exahydrofuro[3s2-fe]pyr^ol-4-yl) ethyl]benzamide 7sT-[l-Benzyl-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-trifluoro methoxybenzamide ^-Dimethylamino-7y/'-[l-ben2yl-2-oxo-2-(3-oxo-liexahydrofuro[3,2-6]pyrrol-4-/l)ethyl]benzamide 7/-[l-Benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)et;hyl]-4-isopropyl benzamide 4-Difluoromethoxy-JVr-[l-ben2yl-2-oxo-2-(3-oxo-liexaliydrofuro[3,2-&]pyrrol-4-yl)ethyl]benzamide A^-[l-Benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-trifluoro methylbenzamide 4-Bromo-JV-[l-benzyl-2-oxo-2-(3-oxo-hexah.ydrofuro[3,2-&]pyrrol-4-yl)ethyl] benzamide 3-Bromo-JV-[l-benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)ethyl] benzamide N-[l-Ben2yl-2-oxo-2-(3-oxo-hexahydroforo[3,2-6]pyrrol-4-yl)ethyl]-4-vinyl benzamide Naphthalene-2-carboxylic acid [l-ben2yl-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-6] pyirol-4-yl)ethyl]amide Naphthalene-1 -carboxylic acid [l-benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe] pyrrol-4-yl)ethyl]amide Quinoline-6-cafboxylic acid [l-benzyl-2-oxo-2-(3-oxo-hexah.ydrofuro[3,2-6] pyrrol-4-yl)ethyl]amide Benzo[£]thiophene-2-carboxylic acid [l-benzyl-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]amide Benzo[6]thiophene-3-carboxylic acid [l-benzyl-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)eihyl]amide Benzothiazole-5-carboxylic acid [l-benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6] pyrrol-4-yl)ethyl] amide Biphenyl-4-carboxylic acid [l-ben2yl-2-oxo-2-(3-oxo-hexaliydrofuro [3,2-6] pyrrol-4-yl)ethyl]amide Ar-[l-Benzyl-2-oxo-2-(3-oxo-hexahydrofliro[352-6]pyiTol-4-yl)ethyl]-4-plienoxy benzamide A'-[l-Benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3J2-fe]pyrrol-4-yl)ethyl]-3-phenoxy benzamide JV-[l-Ben2yl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl3-4-imidazol-l-ylbenzamide JV-[l-Ben2yl-2-oxo-2-(3-oxo-hexahydrofuro[3J2-6]pyrrol-4-yl)ethyl]-4-thiophen-2-ylbenzamide 7/-[l-Benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]-4-oxazol-5-ylbeazamide ^-[l-Benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)etliyl]-4-[l>2,3] thiadiazol-5-ylbenzamide JV-[l-Benzyl-2-oxo-2-(3-oxo-hexahydrofiiro[332-fe]pyrrol-4-yl)etliyl]-4-pyrazol-l-ylbenzamide AT-[l-Benzyl-2-oxo-2-(3-oxo-hexa]iydrofuro[352-&]pyrrol-4-yl)ethyl]-5-tbiophen-2-ylnicotinamide 7V-[l-Ben2yl-2-oxo-2-(3-oxo-liexaliydrofuro[3,2-&]pyrrol-4-yl)ethyl]-6-plienyl nicotinamide 2-Phenyltbiazole-4-carboxylic acid [l-benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide 2-Pyridin-3-ylthiazole-4-carboxylic acid [l-benzyl-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl] amide 5-Phenylthiopb.ene-2-carboxylic acid [l-ben2yl-2-oxo-2-(3-oxo-hexahydrofuro [3,2-&]pyrrol-4-yl)ethyl]amide 5-Pyridin-3-ylthiophene-2-carboxylic acid [l-benzyl-2-oxo-2-(3-oxo-hexahydrofuro 3,2-i]pyrrol-4-yl)eihyl]ainide 2-Methyl-5-phenylfuran-3-cafboxylic acid [ 1 -benzyl-2-oxo-2-(3-oxo-hexahydro furo[3,2-6]pyrrcl-4-yl)ethyl]amide 4-Phenylthiopbene-2-carboxylic acid [l-ben2yl-2-oxo-2-(3-oxo-hexa hydrofuro [3,2-i]pyrrol-4-yl)ethyl]amide 4-Pyridin-3-ylthiophene-2-carboxylic acid [l-ben2yl-2-oxo-2-(3-oxo-hexahydro furo[3J2-^]pyrrol-4-yl)ethyl]amide 2-Thiophen-2-ylthiazole-4-carboxylic acid [ 1 -benzyl-2-oxo-2-(3-oxo-hexahydro furo[3,2-Z>]pyrrol-4-yl)ethyl]amide 2-Methyl-5-(pyrrolidine-l-sulfonyl)furan-3-carboxylic acid [l-benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide 3-Phenylpyrrole-l-carboxylic acid [l-benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3}2-i]pyrrol-4-yl)ethyl]amide 4-[2-(4-fert-Butyl-benzylsulfanyl)-4-methyl-pentanoyl3-tetrahydrofuro[3,2-b3 pyrrol-3 -one 4_[2-(4-fer^-Butyl-benzylsulfanyl)-3-(4-hydroxy-phenyl)-propionyl]-tetrahydro-furo[3,2-b]pyrrol-3-one 2-Isobutyl-l-(3-oxo-hexahydrofuro[3,2-b]pyrrol-4-yl)-4-(3-phenyl-pyrrol-l-yl)-butane-l,4-dione 2-Isobutyl-l:(3-oxo-hexahydrofuro[3,2-b]pyrrol-4-yl)-4-(3-phenyl-pyrrolidin-l-yl)-butane-l,4-dione 2-(4-Hydroxyben2yl)-l-(3-oxo-hexahydrofaro[3,2-b]pyrrol-4-yl)-4-(3-phenyl-pyrrol-1 -yl)-butane-l ,4-dione 2-(4-Hydroxybenzyl)-l-(3-oxo-hexahydrofuro[3,2-b]pyrrol-4-yl)-4-(3-phenyl-pyrrolidin- 1 -yl)-butane- 1 ,4-dione 4-(l,3-Dib.ydro-isoindol-2-yl)-2-isobutyl-l-(3-oxo-hexaliydroforo[3,2-b]pyrrol-4-yl)-butane-1 ,4-dione ,3-Dihydro-isoindol-2-yl)-2-(4-hydroxybenzyl)- 1 -(3-oxo-hexahydrofuro[3 ,2-b]pyrrol-4-yl)-butane-l ,4-dione 4-(3,4-Dihydro-lH-isoquinolin-2-yl)-2-isobutyl-l-(3-oxo-hexahydrofuro[3,2-b]pyrrol-4-yl)-butane-l ,4-dione 4-(3,4-Dihydro-lH-isoquinolm-2-yl)-2-(4-hydroxybenzyl)-l-(3-oxo-hexab.ydro-furo[3,2-b]pyrrol-4-yl)-butane-ls4-dione Further compounds of the invention include, but are not limited to, the following examples that are the (3aS, 7&R) or (3aR, 7aS) isomer of general formula (II), where Z = 'O' and R1 = 'H' and also include the equivalent analogues included in the full definition of Z and R1 Air-[l-(4-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[332-6]pyridin-4-yl)ethyl] benzamide 4-fert-Butyl-7V-[l-(4-liydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-i]pyridm-4-yl)ethyl]benzamide A^[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydroftiro[3,2-6]pyridm-4-yl)ethyl]-4-trifluoromethoxybenzamide 4~Dimethylainino-JV'-[l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fc]pyridin-4-yl)ethyl]benzamide 7V-[l-(4-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)eth.yl]-4-isopropylbenzamide 4-Difluoromethoxy-7V-[l-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyridin-4-yl)ethyl]benzamide 77-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-&]pyridin-4-yl)ethyl]-4-trifluoromethylbenzamide 4-Bromo-JV-[l-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]benzamide 3-Bromo-^V-[l-(4-b.ydroxyben2yl)-2-oxo-2-(3-oxo-hexahydroftiro[3,2-6]pyridin-4-yl)eth.yl]benzamide //-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]-4-vinylbenzamide Naphthalene-2-carboxylic acid [l-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyridin-4-yl)ethyl]amide Naphthalene-1-carboxylic acid [l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-b.exahydrofuro [3,2-fe]pyridin-4-yl)ethyl]amide Quinoline-6-carboxylic acid [l-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyridin-4-yl)ethyl]amide Benzo[6]thiophene-2-carboxylic acid [l-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexa hydrofuro[352-fe]pyridin-4-yl)ethyl]amide Benzo[6]thiophene-3-carboxylic acid [l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-fe]pyridin-4-yl)ethyl]amide Benzothiazole-5-carboxylic acid [l-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-i]pyridin-4-yl)ethyl]amide Biphenyl-4-carboxylic acid [1 -(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-fe]pyridin-4-yl)ethyl]amide ^[l-(4-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyridin-4-yl)ethyl]-4-phenoxybenzamide 7/-[l-(4-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydroforo[3,2-^]pyridin-4-yl)ethyl]-3-phenoxyben2amide 7^-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]-4-imidazol-1 -ylbenzamide AL[l-(4-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexab.ydrofuro[3,2-i]pyridin-4-yl)ethyl]-4-thiophen-2-ylbenzamide oxazol-5-ylbenzamide jV-[l-(4-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]-4-[ 1,2,3]thiadiazol-5-ylbenzamide JV-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydroforo[3,2-6]pyridin-4-yl)eth.yl]-4-pyrazol-1 -ylbenzamide Ar-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[352-6]pyridin-4-yl)ethyl]-5-tbiophen-2-ylnicotinamide JV-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]-6-phenylnicotinamide 2-Phenylthiazole-4-carboxylic acid [l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-fe]pyridin-4-yl)ethyl]amide 2-Pyridin-3-ylthiazole-4-carboxylic acid [ 1 -(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyridin-4-yl)ethyl]amide 5-Phenylthiophene-2-carboxylic acid [l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-^]pyridin-4-yl)ethyl]amide 5-Pyridin-3-yltbiophene-2-carboxylic acid [l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-b.exahydrofuTo[3.)2-fc]pyridin-4-yl)ethyl]ainide 2-Methyl-5-phenylfuran-3-carboxylic acid [l-(4-b.ydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3J2-6]pyridin-4-yl)ethyl]amide 4-Phenylthiophene-2-carboxylic acid [ 1 -(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3s2-&]pyridin-4-yl)ethyl]amide 4-Pyridin-3-ylthiophene-2-carboxylic acid [l-(4-bydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-£]pyridin-4-yl)ethyl]amide 2-Thiophen-2-ylthiazole-4-carboxylic acid [l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyridin-4-yl)ethyl]amide 2-Methyl-5-(pyrrolidine-1 -sulfonyl)fiiran-3-carboxylic acid [ 1 -(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyridin-4-yl)eihyl]amide 3-Phenylpyrrole-l-carboxylic acid [l-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-6]pyridin-4-yl)ethyl]amide ^-[3-Me1hyl-l-(3-oxo-hexahydrofuro[3J2-^]pyridine-4-carbonyl)butyl]benzainide 4-te7^Butyl-7V"-[3-methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl) butyl] benzamide 7V-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl) butyll-4-trifluoro methoxybenzamide 4-Dimethylamino-7/-[3-methyl-l-(3-oxo-hexahydrofuro[3J2-fc]pyridine-4-carbonyl) butyl]benzamide 4-Isopropyl-?/-[3-methyl-l-(3-oxo-hexaliydrofuro[3,2-&]pyridine-4-carbonyl) butyl] benzamide 4-Difluoromethoxy-?/-[3-methyl-l-(3-oxo-liexahydrofuro[3,2-6]pyridine-4-carbonyl) butyljbenzamide Ar-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl) butyl]-4-trifluoro methylbenzamide 4-Bromo-7V-[3-methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-caxbonyl) butyl] benzamide 3-Bromo-JV-[3-metb.yl-l-(3-oxo-hexahydrofuro[352-fe]pyridine-4-carbonyl) butyl] benzamide .V-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-fe]pyridine-4-carbonyl) butyl]-4-vinyl benzamide Naphthalene-2-carboxylic acid [3-memyl-l-(3-oxo-hexahydrofuro[3,2-&]pyridine-4-carbonyl) butyl]amide Naphthalene- 1 -carboxylic acid [3-methyl- 1 -(3-oxo-hexahydrofuro [3 ,2-6]pyridine~4-carbonyl) butyl]amide Quinoline-6-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl) butyl]amide Benzo[fe]thiophene-2-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-i] pyridine-4-carbonyl) butyl]amide Benzo[6]tbiophene-3-carboxylic acid [3-methyl- 1 -(3-oxo-hexahydrofuro[3,2-6] pyridine-4-carbonyl) butyl]amide Benzothiazole-5-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl) butyl] amide Biphenyl-4-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-Z>]pyridine-4-carbonyl) butyl]amide l-l -(3-oxo-hexahydrofuro[3 ,2-6]pyridine-4-carbonyl) butyl]-4-phenoxy benzamide Ar-[3-Methyl-l-(3-oxo-hexahydrofbro[3,2-6]pyridine-4-carbonyl) butyl]-3-phenoxy benzamide 4-Imidazol-l-yl-AT-[3-methyl-l-(3-oxo-hexaliydrofiiro[3,2-6]pyridine-4-carbonyl) butyl]benzamide JV-[3-Methyl-l-(3-oxo-b.exahydrofuro[3,2-&]pyridine-4-carbonyl) butyl]-4-thiophen-2-ylbenzamide JN7-[3-Meihyl-l-(3-oxo-hexahydrofuro[352-6]pyTidine-4-carboiiyl) butyl]-4-oxazol-5-ylbenzamide -V-t3-Methyl-l-(3-oxo-hexaliydrofuro[3,2-&]pyridine-4-carbonyl) butyl]-4-[ 1,2,3] tbiadiazol-4-ylbenzamide JV-[3-Methyl-l-(3-oxo-hexahydrofuro[3)2-6]pyridine-4-carbonyl) butyl]-4-pyrazol-l-ylbenzamide JV-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-cafbonyl) butyl]-5-thiopb.en-2-ylnicotinamide JV-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-63pyridine-4-carbonyl) butyl]-6-phenyl nicotinamide 2-Phenylthiazole-4-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-6] pyridine-4-carbonyl) butyl]amide 2-Pyridin-3-ylthia2ole-4-carboxylic acid [3-methyl-l -(3-oxo-hexahydrofuro[3,2-b]pyridine-4-carbonyl) butyl]amide 5-Phenylthiophene-2-carboxylic acid [3-methyl-1 -(3-oxo-hexahydrofuro[3,2-b] pyridine-4-carbonyl) butyl]amide 5-Pyridin-3-ylthiophene-2-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-&] pyridine-4-carbonyl) butyl] amide 2-Methyl-5-phenylfuran-3-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-&] pyridine-4-carbonyl) butyljamide 4-Phenylthiophene-2-carboxylic acid [3-methyl-1 -(3-oxo-hexahydrofuro [3,2-b] pyridine-4-carbonyl) butyl]amide 4-Pyridin-3-ylthiophene-2-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-6j pyridine-4-carbonyl) butyljamide 2-Trhiophen-2-ylthiazole-4-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-&] pyridine-4-carbonyl) butyl]amide 2-Methyl-5-(pyrrolidine-l-sulfonyl)furan-3-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-&] pyridine-4-carbonyl) butyl]amide 3-Phenylpyrrole-l-carboxylic acid [3-methyl-l -(3-oxo-hexahydrofuro[3,2-6] pyridine-4-carbonyl) buryljamide Additional compounds of the invention include, but are not limited to, the following examples that are the (3aS, 6a#) isomer of general formula (I), where Z = 'O' and R1 = CH', and also include the equivalent analogues included in the full definition of Z andR1 N~ [ 1 -Cyclopropyhnethyl-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]benzamide 4-fe7t-Butyl-JV-[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexaiiydrofuro[3J2-6]pyrrol-4-yl)ethyl]benzamide Ar-[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-trifluoromethoxybenzamide 4-Dimethylamino-7V'-[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexa]iydrofuro[3,2-6]pyrrol-4-yl)etb.yl]benzamide 4-Isopropyl-A7-[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide 4-Difluoromethoxy-7>/-[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-b.exahydrofuro[3,2-fc]pyrrol-4-yl)ethyl]benzamide A7-[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)ethyl]-4-trifluoroinethylbenzainide 4-Bromo-7V"-[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-^]pyrrol-4-yl)ethyl]benzamide 3-Bromo-A7:-[l-Cyclopropylinethyl-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide A-[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydroftiro[3J2-6]pyrrol-4-yl)ethyl]-4-vinylbenzamide Kapb.thalene-2-carboxylic acid [l-Cyclopropylmethyl-2-oxo-2>-(3-oxo- hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide Naphthalene-1-carboxylic acid [l-Cyclopropylmethyl-2-oxo-2-(3-oxo- hexahydrofuro[3,2-fe]pyrrol-4-yl)ethyl]ainide Quinoline-6-carboxylic acid [l-Cyclopropylmethyl-2-oxo-2-(3-oxo- hexab.ydrofuro[3,2-&]pyrrol-4-yl)ethyl]amide Benzo[fe]thiophene-2-carboxylic acid [l-Cyclopropylmeihyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]ainide Benzo[6]thiophene-3-carboxylic acid [l-Cyclopropybnethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide Benzothiazole-5-carboxylic acid [l-Cyclopropylmethyl-2-oxo-2-(3-oxo- hexab.ydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide Biphenyl-4-carboxylic acid [l-Cyclopropylmethyl-2-oxo-2-(3-oxo- hexab.ydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide JV-[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3J2-i]pyrrol-4-yl)ethyl3-4-phenoxy benzamide JV-[l-Cyclopropylmethyl-2--oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)e1:hyl]-3-phenoxy benzamide 4-Imidazol-l -yl-N-[ 1 -Cyclopropylmethyl-2-oxo-2-(3 -oxo-hexahydrofiiro[3,2-6]pyrrol-4-yl)ethyl] benzamide JV"-[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-thiophen-2-ylbenzamide JV-[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-^]pyrrol-4-yl)ethyl]-4-oxazol-5-ylbenzamide ^[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexah.ydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-[1,2,3] thiadiazol-4-ylbenzatnide JV-[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-pyrazol-1 -ylbenzamide ?/-[l-Cyclopropylinethyl-2-oxo-2-(3-oxo-liexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-5-thiophen-2-ylnicotinamide 2V-[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-liexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]-6-phenyl nicotinamide 2-Phenylthiazole-4-carboxylic acid [l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofiiro[3,2-£]pyrrol-4-yl)ethyl]amide 2-Pyridin-3-ylthiazole-4-carboxylic acid [l-Cyclopropylmeihyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide 5-Phenylthiophene-2-carboxylic acid [l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)ethyl]ainide 5-Pyridin-3-ylthiophene-2-carboxylic acid [1 -Cyclopropylmethyl-2-oxo-2-(3-oxo-b.exaliydrofuro[3,2-b] pyrrol-4-yl)ethyl]amide 2-Methyl-5-phenylfuran-3-carboxylic acid [1 -Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-Z»]pyrrol-4-yl)ethyl]amide 4-Phenylthiophene-2-carboxylic acid [l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide c acid [l-Cyclopropylrnethyl-2-oxo-2-(3-oxo-hexahydrofuro[352-6]pyrrol-4-yl)ethyl]amide 2-TMophen-2-ylthiazole-4-carboxylic acid [l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide 2-Methyl-5-(pyrrolidine-l -sulfonyl)furan-3-carboxylic acid [1 -Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide 3-Phenylpyrrole-l-carboxylic acid [l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofiaro[3,2-i]pyrrol-4-yl)ethyl]amide Ar-[l-(3-Oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl)butyl]benzamide 4-tert-'B\tfy\-N-[ 1 -(3-oxo-hexahydrofuro [3,2-6]pyrrole-4-carbonyl) butyl] benzamide N-[l -(3-Oxo-hexahydroforo[3,2-6]pyrrole-4-carbonyl) butyl]-4-trifluoromethoxy benzamide 4-Dimethylanimo-JV'-[l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]benzamide 4-Isopropyl-A^[l-(3-oxo-hexahydroruro[3,2-6]pyrrole-4-carbonyl) butyl] benzamide 4-Difluoromethoxy-7V-[ 1 -(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]benzamide /f-[l-(3-Oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]-4-trifluoromethyl benzamide 4-Bromo-JV-[l -(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl] benzamide i 3-Bromo-Ar-[l -(3-oxo-hexahydrof N-[l -(3-Oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]-4-vinyl benzamide Naphthalene-2-carboxylic acid [1 -(3-oxo4iexahydrofaro[3,2-6]pyrrole-4-carbonyl) butyl]amide Naphthalene- 1-carboxylic acid [l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]amide Quinoline-6-carboxylic acid [1 -(3-oxo-hexahydrofuro[3,2-fc]pyrrole-4-carbonyl) butyl]amide Benzo[fe]tbiophene-2-carboxylic acid [l-(3-oxo-hexahydrofuro[3,2-&]pyrrole-4-carbonyl) butyljamide Benzo[6]tbiophene-3-carboxylic acid [ 1 -(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]amide Benzothiazole-5-carboxylic acid [l-(3-oxo-hexahydrofuro[3,2-i]pyrrole-4-carbonyl) butyl]amide Biphenyl-4-carboxylic acid [l-(3-oxo-nexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]amide JV-[l-(3-Oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl)butyl]-4-phenoxy benzamide 7V-[l-(3-Oxo-hexahydrofuro[3,2-fe]pyrrole-4-carbonyl)butyl]-3-phenoxy benzamide 4-Imidazol-l-yl-]V-[l-(3-oxo-liexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl] benzamide 7V-[l-(3-Oxo-hexahydroforo[3,2-6]pyrrole-4-carbonyl) butyl]-4-thiophen-2-y Ibenzamide AT-[l-(3-Oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]-4-oxazol-5-yl benzamide Ar.[l-(3-Oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]-4-[l,2,3]thiadiazol-4-yl benzamide N-[l-(3-Oxo-hexahydrofuro[3,2-&]pyrrole-4-carbonyl) buty!]-4-pyrazol-l-yl benzamide JV-[l-(3-Oxo-hexahydrofiiro[3,2-6]pyrrole-4-cafbonyl) butyl]~5-thiophen-2-yl nicotinamide A^[l-(3-Oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl)butyl]-6-phenylmcotinamide 2-Phenylihiazole-4-carboxylic acid [l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-cafbonyl) butyl]amide 2-Pyridin-3-yltbiazole-4-carboxylic acid [1 -(3-oxo-hexah.ydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]amide 5-Phenylthiophene-2-cafboxylic acid [l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl] amide 5-Pyridin-3-yltbiophene-2-carboxylic acid [l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl] amide 2-Methyl-5-phenylfuran-3-carboxylic acid [1 -(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl] amide 4-Phenyltbiophene-2-carboxylic acid [1 -(3-oxo-hexahydrofuro[3,2-i]pyrrole-4-cafbonyl) butyl] amide 4-Pyridin-3-ylthiophene-2-carboxylic acid [l-(3-oxo-hexahydrofuro[3,2-fe]pyrrole-4-carbonyl) butyl] amide 2-Thiophen-2-ylthiazole-4-carboxylic acid [l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyljamide 2-Methyl-5-(pyrrolidme-l-sulfonyl)furan-3-carboxylic acid [l-(3-oxo-hexahydro furo[3,2-Z>]pyirole-4-carbonyl)butyl]amide 3-Phenylpyrrole-l-carboxylic acid [l-(3-oxo-hexahydrofuro[3,2-6]pyrrole -4-carbonyl) butyl]amide 4-Memyl-JV-[3-methyl-l-(3-oxo-hexahydrofuro[3,2-b]pyrrole-4-carbonyl) butyl] ben2amide 4-Methoxy-JV'-[3-methyl-l-(3-oxo-hexahydrofuro[3,2-b]pyrrole-4-carbonyl) butyl] benzamide JV-[3-Memyl-l-(3-oxo-hexahydrofuro[3,2-b]pyrrole-4-carbonyl)butyl]-4-morpholin-4-ylbenzamide 4-fe^Butyl-A^[l-(4-methoxybenzyl)-2-oxo-2-(3-oxo-hexab.ydrofUro[3J2-i]pyrrol-4-yl)ethyl]benzamide Naphthalene-2-carboxylic acid [ 1 -(4-methoxybenzyl)-2-oxo-2-(3 -oxo-hexahydro furo[3,2-b]pyrrol-4-yl)ethyl]amide Biphenyl-4-cafboxylic acid [l-(4-methoxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-fe]pyrrol-4-yl)ethyl] amide 4-te/^-Butyl-//-[l-(4-liydroxyniethylbenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide Naphthalene-2-carboxylic acid [l-(4-hydroxymethylbenzyl)-2-oxo-2-(3-oxo-hexahydrofaro[3,2-£]pyrrol-4-yl)ethyl]amide Biphenyl-4-carboxylic acid [l-(4-hydroxymethylbenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide Furan-2-carboxylic acid [1 -(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-Z?]pyrrol-4-yl)ethyl] amide Furan-3-carboxylic acid [l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl] amide Thiophene-2-carboxylicacid[l-(4-liydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]amide Tbiophene-3-carboxylicacid[l-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]amide Morpholine-4-carboxylic acid [ 1 -(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-fc]pyrrol-4-yl)ethyl]amide ^V'-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-2-phenylacetamide JV-[l-(4-Hydroxyben2yl)-2-oxo-2-(3-oxo-liexaliydrofuro[3,2-&]pyrrol-4-yl)ethyl]-3-phenylpropionamide Furan-2-cafboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-fc]pyrrole-4-cafbonyl) butyl]amide Furan-3-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-i]pyrrole-4-carbonyl) butyl] amide Thiophene-2-carboxylicacid[3-methyl-l-(3-oxo-hexahydrofuro[3,2-^]pyrrole-4-carbonyl) butyl]amide Thiophene-3-carboxylic acid [3-methyl-l-(3-oxo-hexahydroiuro[3,2-fe]pyrrole-4-cafbonyl) butyl]amide Morpholine-4-carboxylicacid[3-methyl-l-(3-oxo-b.exahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]amide ^-[3-Methyl-l-(3-oxo-hexahy6^ofuro[3,2-fe]pyrrole-4-carbonyl)butyl]-2-phenyl acetamide /^-[S-Methyl-l-CS-oxo-hexahydrofurotS^-ijpyrrole^-carbonyObutyll-S-phenyl propionamide Furan-2-carboxylic acid [1 -cyclohexylmethyl-2-oxo-(3-oxo-hexahydrofuro[3,2-b] pyrrol-4-yl)ethyl]amide Furan-3-carboxylic acid [l-cyclohexylmethyl-2-oxo-(3-oxo-hexahydroruro[3,2-6]pyrrol-4-yl)ethyl]amide Thiophene-2-carboxylic acid [l-cyclohexyhnethyl-2-oxo-(3-oxo-hexahydro furo[3,2-6]pyrrol-4-yl)ethyl]amide Thiophene-3-carboxylic acid [l-cyclohexylmethyl-2-oxo-(3-oxo-hexahydro furo[3,2-6]pyrrol-4-yl)ethyl] amide Morpholine-4-carboxylicacid[l-cyclohexylmethyl-2-oxo-(3-oxo-hexahydro foro[3,2-£]pyrrol-4-yl)ethyl]amide A^-[l-Cyclohexylmethyl-2-oxo-(3-oxo-liexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-2-phenyl acetamide N-[l-Cyclohexylmetiiyl-2-oxo-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-3-phenylpropionamide Ar-[l-Cyclohexylmethyl-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl] benzamide Naphthalene-1 -carboxylic acid [ 1 -cy clohexylmethyl-2-oxo-2-(3 -oxo-hexahydro furo[3,2-3]pyrrol-4-yl)ethyl]amide Benzo[6]thiophene-2-cafboxylic acid [1 -cyclohexyhnethyl-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide Furan-2-carboxylicacid[3,3-dimethyl-l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]amide Furan-3-carboxylicacid[3,3-dimethyl-l-(3-oxo-hexahydrofuro[352-63pyrrole-4-carbonyl) butyl]amide Thiophene-2-carboxylic acid [3,3 -dimethyl-1 -(3-oxo-hexahydrofuro [3,2-6]pyrrole-4-carbonyl) butyl]amide Thiophene-3-carboxylic acid [3,3-dimethyl-l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl] amide Morpholine-4-carboxylic acid [3,3-dimethyl-l -(3-oxo-hexahydrofuro[3,2-i]pyrrole-4-carbonyl) butyljamide Ar-[3^-Dimethyl-l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl)butyl]-2-plienyl acetamide JV-[3,3-Dimethyl-l-(3-oxo-hexaliydrofuro[3,2-6]pyrrole-4-carbonyl)butyl]-3-p1ienyl propionamide Benzo[6]thiophene-2-carboxylicacid[3,3-dimethyl-l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyljamide JV-[33-Dimethyl-l-(3-oxo-hexaliydrofuro[3,2-Z>]pyrrole-4-carbonyl) butyl] benzamide Furan-2-carboxyHcacid[l-ben27l-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl) ethyl]amide Furan-3-carboxylicacid[l-ben2yl-2-oxo-2-(3-oxo-hexab.ydrofuro[3,2-6]pyrrol-4-yl) ethyl]amide Thiophene-2-carboxylicacid[l-ben2yl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-£>]pyrroi-4-yl)ethyl]amide Thiophene-3-carboxylic acid [ 1 -benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)ethyl] amide Morpholine-4-carboxylic acid [1 -benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&] pyrroI-4-yl)ethyl]amide JV-[l-Benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)eihyl]-2-plienyl acetamide propionamide Benzo[6]tliiophene-2-carboxylicacid[l-benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]amide A^-[l-Ben2yl-2-oxo-2-(3-oxo-hexahydrof\ro[3,2-6]pyrrol-4-yl)ethyl]ben2amide Furan-2-carboxylicacid[l-cyclopentylmethyl-2-oxo-(3-oxo-liexahydrofuro[3,2-6] pyrrol-4-yl)ethyl]amide Furan-3-cafboxylic acid [l-cyclopentylmethyl-2-oxo-(3-oxo-hexahydro furo[3,2-&]pyrrol-4-yl)ethyl]amide Thiophene-2-carboxylic acid [l-cyclopentylmethyl-2-oxo-(3-oxo-hexahydro furo[3,2-i]pyrrol-4-yl)ethyl]amide Thiophene-3-carboxylic acid [l-cyclopentylmethyl-2-oxo-(3-oxo-hexahydro furo[3,2-i]pyrrol-4-yl)ethyl]amide Morpholine-4-carboxylic acid [l-cyclopentylmethyl-2-oxo-(3-oxo-hexahydro furo[3,2-&]pyrrol-4-yl)ethyl]amide A^-[l-Cyclopentylmeihyl-2-oxo-(3-oxo-hexahydrofuro[3,2-fo]pyrrol-4-yl)ethyl]-2-phenylacetamide A?-[l-Cyclopentylinethyl-2-oxo-(3-oxo-hexaliydrofuro[3,2-6]pyrrol-4-yl)ethyl]-3-phenylpropionamide N-[l -Cyclopentylmethyl-2-oxo-2-(3-oxo-hexahydroforo [3,2-6]pyrrol-4-yl)ethyl] benzamide Naphthalene-1-carboxylic acid [l-cyclopentylmetliyl-2-oxo-2-(3-oxo-liexahydro furo[3 ,2-&]pyrrol-4-yl)ethyl]atnide Benzo[6]thiophene-2-carboxylic acid [l-cyclopentylmethyl-2-oxo-2-(3-oxo-hexa Furan-2-carboxylic acid [l-benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexaliydro furo[3 ,2-6]pyrrol-4-yl)ethyl]amide Furan-3-carboxylic acid [l-benzylsulfanyLmethyl-2-oxo-2-(3-oxo-hexahydro furo[332-fe]pyrrol-4-yl)ethyl]amide Thiophene-2-carboxylic acid [l-benzylsulfanylmethyl-2-oxo-2-(3-oxo-liexahydro furo[3,2-fe]pyrrol-4-yl)ethyl]aimde Tbiophene-3-carboxylic acid [l-benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexaliydro furo[3,2-fc]pyrrol-4-yl)ethyl]amide Morpholine-4-carboxylic acid [1 -ben2ylsulfanylmethyl-2-oxo-2-(3-oxo-liexahydro furo[3 ,2-&]pyrrol-4-yl)ethyl]amide Ar-[l-Beiizylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydrofurot3,2-63pyrrol-4-yl)ethyl3-2-phenylacetamide A^-[l-Benzylsulfanylme1iiyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-3-pb.enylpropionamide Benzo[fe]thiophene-2-carboxylic acid [1 -ben2ylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]ainide 7^-[l-Benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexah.ydrofuro[3,2-6]pyrrol-4-yl) ethyl]benzamide Naphthalene-1 -carboxylic acid [l-ben2ylsulfanylmethyl-2-oxo-2~(3-oxo4iexahydro foro[3,2-6]pyrrol-4-yl)ethyl]amide Furan-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l -(phenylmethanesulfonylmethyl)ethyl]amide Furan-3-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-(phenylmethanesulfonylmetliyl)eihyl]ainide Thiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-1 -(phenylmethanesulfonylmethyl)ethyl]amide Thiophene-3-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-(phenylmethanesulfonylmethyl)ethyl]amide Morpholine-4-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-1 -(phenylmethanesulfonylmethyl)eihyl]amide JV-[2-Oxo-2-(3-oxo-liexahydrofuro[3,2-6]pyrrol-4-yl)-l-(phenylmethanesulfonyl methyl)ethyl]-2-phenylacetamide 7V-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-i]pyrrol-4-yl)-l-(phenylmethanesulfonyl methyl)ethyl]-3-phenylpropionamide Benzo[6]thiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3)2-6]pyrrol-4-yl)-l-(phenylmethanesulfonylmethyl)ethyl]ainide JV-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-1 -(phenylmethanesulfonyl methyl)ethyl]benzamide Naphthalene-1-carboxylic acid [2-oxo-2-(3-oxo-b.exahydrofuro[3,2-6]pyrrol-4-yl)-l-(phenylmethanesulfonylmethyl)ethyl]ainide 4-(2-Benzyloxy-3-cyclohexyl-propionoyl)tetrahydrofuro[352-6]pyrrol-3-one 4-[2-(4-terr-Butylbenzyloxy)-4-methyl-pentanoyl]tetrahydrofuro[3,2-b]pyrrol-3-one 4-[2-(Naphthalen-l-ylmethoxy)-3-phenyl-propionyl]-tetrahydro-furo[3,2-b]pyrroi-3-one 4-[3-Cyclohexyl-2-(foran-2-ylmethanesulfanyl)propionoyl]tetrah.ydrofuro[3j2-b]pyrrol-3-one 4-[3-Cyclohexyl-2-(furan-3-ybnethanesulfanyl)propionoyl]tetrahydrofiiro[3,2-b]pyrrol-3-one 4-[3-C^clohexyl-2-(furan-2-ylmetbanesulphonyl)propionoyl]tetrahydrofuro[3 b]pyrrol-3-one 4-[3-C^clohexyl-2-(furan-3-ylmethanesulphonyl)propionoyl]tetrab.ydrofuro[3,2-b]pyrrol-3-one Morpholine-4-carboxylic acid 1 -cyclohexylmethyl-2-oxo-2-(3-oxo-hexahydro-furo [3,2-b]pyrrol-4-yl)-ethyl ester Morpb.oline-4-cafboxylic acid 3-methyl-l-(3-oxo-b.exahydrofuro[3,2-b]pyrrole-4-carbonyl)-butyl ester Morpholine-4-cafboxylic acid 3,3-dimemyl4-(3-oxo-hexahydrofuro[3,2-b] pyrrole-4-carbonyl)-butyl ester 2-Cyclohexylmethyl-4-morpholin-4-yl-l-(3-oxo-hexaliydroftiro[3,2--b3pyrrol-4-yl)butane-l ,4-dione 2-Isobutyl-4-morpholin-4-yl-l-(3-oxo-hexahydrofuro[3,2-b]pyrrol-4-yl)-butane-l,4-dione 2-(2,2-Dimethyl-propyl)-4-morpholin-4-yl-l-(3-oxo-hexahydrofuro[352-b]pyrrol-4-yl)-butane-l ,4-dione 4-(2-Biphenyl-3-yl-4-methylpentanoyl)tetrab.ydrofuro[3,2-b]pyrrol-3-one Further additional compounds of the invention include, but are not limited to, the following examples that are the (3aS, 7aR) or (3afl, 7aS) isomer of general formula (II) where Z = 'O' and R1 = 'H', and also include the equivalent analogues included in the full definition of Z and R1 Furan-2-carboxylicacid[l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3>2-&]pyridin-4-yl)ethyl]amide Furan-3-cafboxylicacid[l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]amide Thiophene-2-carboxylic acid [ 1 -(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-&]pyridin-4-yl)ethyl]amide Thiophene-3-carboxylicacid[l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3)2-i]pyridin-4-yl)ethyl]amide Morpholine-4-carboxylic acid [ 1 -(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-i]pyridin-4-yl)et:hyl]amide j\-.[ 1 -(4-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]-2-phenylacetamide ^•.[l_(4_Hydroxybenzyl)-2-oxo-2-(3-oxo-liexahydrofuro[3,2-6]pyridin-4-yl)ethyl]-3-phenylpropionamide A"-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl] benzamide Fnran-2-carboxylicacid[3-methyl-l-(3-oxo-hexahydrofuro[3,2-fr]pyridine-4-cafbonyl) butyl]amide Furan-3-carboxylic acid [3-meth.yl-l-(3-oxo-h.exahydrofuro[3,2-6]pyridine-4-carbonyl) butyl]amide TMophene-2-c^boxylicadd[3-methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl) butyl]amide Thiophene-3-carboxylic acid [3-methyl-l -(3-oxo-hexahydrofuro[3,2-fc]pyridine-4-cafbonyl) butyl]amide Morpholine-4-carboxylicacid[3-methyl-l-(3-oxo-liexaliydrofuro[3,2-6]pyridine-4-carbonyl) butyl]amide A7-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl)butyl]-2-phenyl acetamide JV>[3-Methyl-l-(3-oxo-hexah.ydrofuro[3,2-6]pyridine-4-carbonyl)butyl]-3-plienyl propionamide Furan-2-carboxylicacid[l-cyclohexylmetliyl-2-oxo-(3-oxo-hexaliydrofuro[3,2-fe] pyridin-4-yl)eihyl]amide Furan-3-carboxylicacid[l-cycloliexylmethyl-2-oxo-(3-oxo-hexahydroftiro[3,2-6]pyridin-4-yl)ethyl]amide Thiophene-2-cafboxylic acid [l-cyclohexylmethyl-2-oxo-(3-oxo-hexahydro furo[3,2-6]pyridin-4-yl)ethyl]amide Thiophene-3-carboxylic acid [l-cyclohexyhnethyl-2-oxo-(3-oxo-hexahydro faro[3,2-&]pyridin-4-yl)ethyl]amide Morpholine-4-carboxylic acid [l-cyclohexylmethyl-2-oxo-(3-oxo-hexahydro furo[3,2-fc]pyridin-4-yl)ethyl]amide JV"-[l-Cyclohexylmethyl-2-oxo-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]-2-phenylacetamide 7/-[l-Cyclohexyhiiethyl-2-oxo-(3-oxo-hexahydrofuro[3,2-6]pyridm-4-yl)ethyl]-3-phenylpropionamide N-[ 1 -Cyclohexyhnethyl-2-oxo-2-(3-oxo-hexahydrofuro [3,2-ib]pyridin-4-yl)ethyl] benzamide Naphthalene-1-carboxylic acid [l-cyclohexylmethyl-2-oxo-2-(3-oxo-hexahydro furo[3,2-fo]pyridin-4-yl)ethyl]amide Benzo[6]thiophene-2-carboxylic acid [1 -cyclohexylmethyl-2-oxo-2-(3-oxo-hexa hydrofuro[3}2-&]pyridin-4-yl)ethyl]amide Furan-2-carboxylicacid[3,3-dimethyl-l-(3-oxo-hexahydroforo[3,2-&]pyridine-4-carbonyl) butyl]amide Furan-3-carboxylicacid[3,3-dimethyl-l-(3-oxo-hexahydrofuro[3,2-&]pyridine-4-carbonyl) butyl]amide Thiophene-2-carboxylic acid [3 ,3 -dimethyl- 1 -(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl) butyl]amide Tbiophene-3-carboxylicacid[3,3-diiiiethyl-l-(3-oxo-hexahydrofuro[3,2-63pyridine-4-carbonyl) butyl] amide Morpholine-4-carboxylicacid[33-dimethyl-l-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl) butyl] amide 7/-[33-Dimetiiyl-l-(3-oxo-hexahydrofuro[3,2-fe]pyridine-4-carbonyl)butyl]-2-phenyi acetamide ?/-[33-Dimethyl-l-(3-oxo-hexahydrofuro[3,2-fe]pyridiiie-4-carbon.yl)butyl]-3-phenyl propionamide Benzo[6]thiophene-2-carboxylicacid[3,3-dimethyl-l-(3-oxo-liexahydrofuro[3,2- utyl]amide 7V-[3,3-Dimethyl-l-(3-oxo-hexahydrofuro[3,2-i>]pyridine-4-carbonyl) butyl] benzamide Furan-2-carboxylic acid [ 1 -ben2yl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyridin-4-yl) etbyl]amide Furan-3-carboxylicacid[l-benzyl-2-oxo-2-(3-oxo-liexahydrofaro[3,2-fe]pyridin-4-yl) ethyl] amide Thiophene-2-carboxylic acid [l-ben2yl-2-oxo-2-(3-oxo-hexahydrofuxo[3,2-t]pyridin-4-yl)ethyl] amide Tbiophene-3-cafboxylicacid[l-ben2yl-2-oxo-2-(3-oxo-liexahydrofuro[3s2-6]pyrrol-4-yl)ethyl]amide Morpholine-4-carboxylic acid [1 -benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&] pyridin-4-yl)ethyl]amide AT-[l-Ben2yl-2-oxo-2-(3-oxo-liexahydrofuro[3,2-ib]pyridin-4-yl)ethyl]-2-phenyl acetamide //-[l-Ben2yl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-£)]pyridm-4-yl)ethyl]-3-phenyl propionamide Benzo[fc]thiophene-2-carboxylic acid [1 -ben2yl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]amide 7f-[l-ben2yl-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-fc]pyridin-4-yl)ethyl]benz Furan-2-carboxylicacid[l-cyclopentylmeihyl-2-oxo-(3-oxo-hexab.ydrofuro[3j2-£»] pyridin-4-yl)ethyl]amide Furan-3-carboxylic acid [l-cyclopeaitylme1;hyl-2-oxo-(3-oxo-b.exahydro turo[3,2-fe]pyridin-4-yl)ethyl]amide Thiophene-2-carboxylic acid [l-cyclopentylmethyl-2-oxo-(3-oxo-hexahydro furo[3,2-&]pyridin-4-yl)eihyl]amide Thiophene-3-carboxylicacid[l-cyclopentyhiiethyl-2-oxo-(3-oxo-hexahydro furo[3,2-6]pyridin-4-yl)ethyl]amide Morpholine-4-carboxylicacid[l-cyclopentylmethyl-2-oxo-(3-oxo-hexahydro furo[3 52-&]pyridm-4-yl)ethyl]amide ^-[l-Cyclopentylmethyl-2-oxo-(3-oxo-hexahydrofuro[3,2-Z>]pyridin-4-yl)ethyl]-2-phenylacetamide JV-[l-Cyclopentylmethyl-2-oxo-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]-3-phenylpropionamide JV-[l-Cyclopentylmethyl-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyridin-4-yl)ethyl] benzamide Naphthalene- 1 -carboxylic acid [1 -cyclopentylmethyl-2-oxo-2-(3-oxo-hexahydro furo[3,2-6]pyridin-4-yl)eihyl]amide Benzo[6]ihiophene-2-carboxylic acid [1 -cyclopentylmethyl-2-oxo-2-(3-oxo-hexa hydrofiiro[3 ,2-6]pyridin-4-yl)ethyl]amide F\iran-2-carboxylic acid [l-benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydro furo[3,2-^]pyridin-4-yl)ethyl]amide Furan-3-carboxylic acid [l-benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydro Thiophene-2-carboxylic acid [1 -benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydro furo[3,2-6]pyridin-4-yl)ethyl]amide Thiophene-3-carboxylic acid [l-benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydra furo[3,2-6]pyridin-4-yl)ethyl]amide Morpholine-4-carboxylic acid [l-ben2ylsxdfanylmethyl-2-oxo-2-(3-oxo-hexahydro fiaro[3,2-i]pyridin-4-yl)ethyl]amide N-[ 1 -Benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-i]pyridin-4-yl)ethyl]-2-phenylacetamide N-[ 1 -Ben2ylsulfanylmetliyl-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-6]pyridin-4-yl)ethyl]-3-plienylpropionainide Benzo[fc]thiophene-2-carboxylic acid [1 -benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]ainide JV"-[l-Benzylsulfanylmeihyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-Z)]pyridin-4-yl) ethyljbenzamide Naphthalene-1-carboxylic acid [l-benzylsulfanybnethyl-2-oxo-2-(3-oxo-hexahydro furo[3,2-i]pyridin-4-yl)ethyl]amide Furan-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofijro[3,2-fe]pyridin-4-yl)-l-(phenyhnethanesulfonylmethyl)ethyl]amide Furan-3-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)-l-(phenykQethanesulfonybnethyl)ethyl]amide Thiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)-l-(phenylmetiianesiilfonyhnethyl)ethyl]amide Thiophene-3-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyridin-4-yl)-l-(phenylmethanesulfonyhnethyl)etiiyl]ainide Morpholiiie-4-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)-l-(phenyhnethanesulfonyhnethyl)ethyl]ainide 7V-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)-l-(phenylmethanesulfonyl methyl)ethyl]-2-phenylacetamide Ar-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyridin-4-yl)-l-(plienylinethanesulfonyl methyl)ethyl]-3-phenylpropionamide Benzo[6]thiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)-1 -(phenylmethanesulfonylmethyl)ethyl]amide 7V-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-fc]pyridin-4-yl)-l-(phenylmethanesulfonyl inethyl)ethyl]benzamide Naphthalene-1 -carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)-1 -(phenyhnethanesulfonyhnethyl)ethyl]amide MorphoUne-4-carboxylic acid l-cyclohexylmethyl-2-oxo-2-(3-oxo- hexahydrofuro[3,2-fc]pyridin-4-yl)ethyl ester 4-[3-Cyclohexyl-2-(furan-2-ybiethanesulfanyl)propionyl]hexahydrofuro[3J2-b] pyridin-3-one 4-[3-Cyclohexyl-2-(furan-2-yhnethanesulphonyl)propionyl]hexahydrofuro[3,2-b]pyridin-3-one 4-[3-Cyclohexyl-2-(furan-3-ylmethanesulfanyl)propionyl]hexahydrofuro[3s2-b] pyridin-3-one 4-[3-Cyclohexyl-2-(furan-3-ybnethanesulphonyl)propionyl]hexahydrofuro[3,2-b]pyridin-3-one 2-Cyclohexylmethyl-4-morpholin-4-yl-l-(3-oxo-hexahydrofuro[3,2-b]pyridm-4-yl)butane-l ,4-dione Considering all of the above examples, it is also intended to include the oxidised analogues of capping groups that contain a readily oxidised nitrogen to give the N-oxide or a readily oxidised sulphur to give the sulphone. The following structures are illustrative examples;To those skilled in the practices of organic chemistry, compounds of general formulae (I) and (II) may be readily synthesised by a number of chemical strategies, performed either in solution or on the solid phase (see Atherton, E. and Sheppard, R. C. In 'Solid Phase Peptide Synthesis: A Practical Approach', Oxford University Press, Oxford, U.K. 1989, for a general review of solid phase synthesis principles). The solid phase strategy is attractive in being able to generate many thousands of analogues, typically on a 5-100mg scale, through established parallel synthesis methodologies (e.g. see (a) Bastos, M.; Maeji, N. J.; Abeles, R. H. Proc. Natl Acad. Sci. USA, 92,6738-6742,1995). Therefore, one strategy for the synthesis of compounds of general formulae (I) and (El) comprises:- (a) Preparation of an appropriately functionalised and protected bi-cyclic ketone building block in solution. (b) Attachment of the building block (a) to the solid phase through a linker that is stable to the conditions of synthesis, but readily labile to cleavage at the end of a synthesis (see James, I. W., Tetrahedron, 55(Report N9- 489), 4855-4946, 1999, for examples of the 'linker' function as applied to solid phase synthesis). (c) Solid phase organic chemistry (see Brown, R. D. /. Chem. Soc., Perkin Trans.1, 19, 3293-3320,1998), to construct the remainder of the molecule. (d) Compound cleavage from the solid phase into solution. (e) Cleavage work-up and compound analysis. The first stage in a synthesis of compounds of general formulae (I) and (II) is the preparation in solution of a functionalised and protected building block. A typical scheme towards the tetrahydrofuro[3^-6]pyrrol-3-one (19) is detailed in Scheme 1. The following descriptions detailed in Schemes 1-13 could equally be applied using alternative scaffolds of general formulae (I) and (II). Scheme 1. (a) 'BuOCOCl, NMM, DCM, -15°C, lOmins, under argon, (b) Diazomethane in diethyl ether, -15°C to RT over Ihr. (c) Acetic acid (d) LiCl (lOeq) in 80%aq acetic acid, 5°C to RT over Ihr. FmOC(O) denotes the well known amine protecting group 9-fluorenyl methoxycarbonyl (Fmoc, see Atherton, E. and Sheppard, R. C., 1989) and Tg' denotes either a free hydroxyl or an hydroxyl protecting group such as tertJoutyl ether. In the illustrated case, condensation with diazomethane provides R1 = H. Although formation of the diazoketone is clearly observed at Ihr reaction, an overall improvement in isolated yield is obtained by leaving the reaction with ethereal diazomethane for 24hr. Considering step (a), synthesis may commence from suitably protected p-hydroxyproline (17) (or protected (3-hydroxypipecolic acid), which are accessible through a variety of literature methods e.g. (a) Heffher, R. J., et al, J. Am. Chem. Soc., 114, 10181-10189, 1992; (b) Hughes, P. Cardy, J., /. Org. Chem, 54, 3260-3264, 1989, (c) Hefmer, R. J, Jouille, M. M., Tet. Lett, 30, 7021-7024, 1989, (d) Ewing, W. R., Jouille, M. M., Heterocydes, 27, 2843-2850, 1988. (e) Kolodziej, S. A., Marshall, G. R., Int. J. Pept. Prot. Res., 48, 274-280, 1996, (f) Evans, D. A., Weber, A, E., J. Am. Chem. Soc, 109. 7151-7157, 1987, (g) Langlois, N., Rakotondradany, F., Tetmhedron, 56, 2437-2448, 2000, (h) Sugisaki, C. H., et al, Tet. Lett., 39, 3413-3416, 1998, (i) Greek, C., et al, Tet. Lett, 37, 2031-, 1996, (j) Agami, C., et al, Tet. Lett., 37, 4001 -, 1996. Activation of the suitably protected p-hydroxyproline (17) via isobutyl chloroformate mixed anhydride, followed by condensation with diazomethane, yields the diazomethylketone intermediates (18). Treatment of diazomethylketone intermediates (18) with lithium chloride in aqueous acetic acid provides the protected tetrahydroforo[3,2-6]pyrrol-3-one (19). Introduction of simple R1 substituents may be achieved by condensation of activated (17) with alternatives to diazomethane such as diazoethane (R1 = CHs), or 1-phenyloxydiazoethane (R1 = The protected building blocks (synthesis exemplified by the tetrahydrofuro[3,2-&]-pyrrol-3-one (19)) detailed in Scheme 1 may be utilised in a solid phase synthesis of inhibitor molecules (steps (b) to (e)). Step (b), the solid phase linkage of an aldehyde or ketone, has previously been described by a variety of methods (e.g. see (a) James, I. W., 1999, (b) Lee, A., Huang, L., Ellman, J. A., J. Am. Chem. Soc, 121(43), 9907-9914, 1999, (c) Murphy, A. M., et al, J. Am. Chem. Soc, 114, 3156-3157, 1992). A suitable method amenable to the reversible linkage of an alkyl ketone functionality such as (19) is through a combination of the previously described chemistries. The semicarbazide, 4-[[(hydrazinocarbonyl)amino]methyl]cyclohexane carboxylic acid. trifluoroacetate (20) (Murphy, A. M., et al, J. Am. Chem. Soc, 114, 3156-3157, 1992), may be utilised as illustrated in Scheme 2, exemplified by linkage of the tetrahydrofuro[3,2-6]pyrrol-3-one(19). Scheme 2. (a) (19) in 90% EtOH / H2O / 1.5eq NaOAc / 4-[[(hydrazinocarbonyl)amino]methyl]-cyclohexane carboxylic acid-trifluoroacetate (20), 2hr reflux, (b) 3eq construct (21) / 3eq HBTU / 3eq HOBt / 6eqNMM, NH2-SOUDD PHASE, DMF, RT, o/n. (c) 20% piperidine / DMF, 30mins. (d) Range of chemistries to introduce U-V-W-X-Y (e) TFA / H20 (95:5, v/v), RT, 2hr Construct (21) is prepared through reaction of the linker molecule (20) and the tetrahydrofuro[3,2-£]pyrrol-3-one (19) by reflux in aqueous ethanol / sodium acetate. Standard solid phase techniques (e.g. see Atherton, E. and Sheppard, R. C., 1989) are used to anchor the construct to an amino-functionalised solid phase through the free carboxylic acid functionality of (21), providing the loaded construct (22). Loaded construct (22) may be reacted with a wide range of carboxylic acids available commercially or in the literature, to introduce the left-hand portion 'U-V-W-X-Y' in general formula (I). In the simplest example, the entire left hand portion of an inhibitor of general formula (I) comprises a capped aminoacid (Scheme 3), providing for example analogues of general formula (I) where R12 = 'H', (X)0 = '-', (W)n = 'NH', R16 = 'H', n = 1, (V)m = 'CO', m = 1 and U = aryl 40eq NMM, DMF, o/n (c) 5eq carboxylic acid / 5eq HBTU / 5eq HOBt / lOeq NMM, DMF, RT, o/n (d) TFA / H20 (95:5, v/v), RT, 2hr. Alternatively, carboxylic acids can be prepared in solution by traditional organic chemistry methods and coupled to construct (22) on the solid phase (Schemes 4-8). For example (Scheme 4), treatment in solution of an amino acid, exemplified by (24) with sodium nitrite / t^SCU, provides the a-hydroxyacid, exemplified by (25) (Degerbeck, F. et al, J. Chem. Soc, Perkin Trans. J, 11-14,1993). Treatment of cc-hydroxyacid, (25) with sodium hydride in a dimethylformamide / dichloromethane mixture followed by addition of benzyl bromide, provides IRS-benzyloxy-S-cyclohexylpropionic acid (26). Coupling of (26) to the solid phase construct (22) followed by cleavage, provides (27), an example of general formula (I) where R12 -'H', (X)0 = '-', (W)n = 'O', n = 1, (V)m = 'CH2', i.e. R17, R18 = 'H', m 1 and U = phenyl. To those skilled in the practices of organic synthesis, a wide variety of aminoacids such as (24) may be converted to the corresponding a-hydroxyacid such as (25) following the general conditions detailed. Additionally, benzylbromide may be replaced by any reasonable Ar-CR17R18-halide, providing many variations of carboxylic acid (26) following the general conditions detailed. In certain instances, it may be advantageous to temporarily protect the carboxylic acid as the methyl ester (for example compound (32), Scheme 6) prior to reaction with the alkylhalide. The ester intermediate is then simply hydrolysed to acid (26). Analogues of (27), exploring a wide range of (V)m and U in general formula (I) may be prepared through the general conditions detailed in Scheme 4. Since the final synthetic step involves a trifluoroacetic acid (TFA) mediated cleavage of the solid phase bound compound, analogues where the substituted ether is labile to TFA may be prepared in solution by an alternative route (see Scheme 11). Scheme 4. (a)NaNO2 / H2SO4,0°C-»RT, 2hr (b) 2.3eqNaH, 1:1 DMF /DCM, 1.4eqbenzylbromide, o/n (c) 20% piperidine / DMF, SOmins. (d) lOeq (26) / lOeq HBTU / lOeq HOBt / 20eq NMM, DMF, RT, o/n (e) TFA / H2O (95:5, v/v), RT, 2hr. Alternatively, coupling of construct (22) (following removal of Fmoc) with the ct-hydroxyacid (25), provides a versatile solid phase bound intermediate' Y' substituent in general formula (I) that may be reacted with many reagents. For example, the o> hydroxyl can be reacted under Mitsunobu conditions (Hughes, D. L. Org. React.(N.Y), 42, 335-656, 1992) to give ethers (i.e. X = '-', W = 'O', in general formula (I)) (see Grabowska, U. et al, J. Comb. Chem., 2(5), 475-490, 2000, for an example of Mitsunobu reaction on the solid phase). Alternatively, the a-hydroxyl can be reacted with a carbamoyl chloride to give a carbamate (i.e. X = '-', W = O', V = 'NHC(O)', in general formula (I)). Alternatively, (Scheme 5), treatment in solution of an ammo acid, exemplified by (24) with sodium nitrite / H2S04 / potassium bromide provides the a-bromoacid, exemplified by (28) (Souers, A. J. et al, Synthesis, 4, 583-585,1999) with retention of configuration. Treatment of a-bromoacid (28) with an alkylthiol exemplified by 4-^rt-butylphenylmethanethiol (29) in dimethylformamide / triemylamine, provides 25r-(4-tert-butylbenzylsulfanyl)-4-methylpropionic acid (30), with inversion of configuration. Coupling of (30) to the solid phase construct (22) followed by cleavage, provides (31), an example of general formula (I) where R12 = 'H', (X)0 = '-', (W)n = 'S', n = 1, (V)m = 'CH2', i.e. R17, R18 = 'H', m = 1 and U = 4-tert-butylphenyl. To those skilled in the practices of organic synthesis, a wide variety of aminoacids such as (24) may be converted to the corresponding a-bromoacid such as (28) following the general conditions detailed. Additionally, starting with the S-isomer of (24) gives the S-bromoacid analogue of (28) and jR-thioether analogue of (30). Additionally, (4-ter/-butylphenyl)methanethiol (29) may be replaced by any reasonable Ar-CR17R18-SH, providing many variations of carboxylic acid (30) following the general conditions detailed. Thus analogues of (31) exploring a wide range of (V)m and U in general formula (I) may be prepared through the general conditions detailed in Scheme 5. Scheme 5. (a)NaNO2 / H2SO4l KBr 0°C-»RT, 2hr (b) Alkylthiol (29) / DMF / NEt3) o/n (c) 20% pipendine / DMF, SOmins. (d) lOeq (30) / lOeq HBTU / lOeq HOBt / 20eq NMM, DMF, RT, o/n (e) TFA / H2O (95:5, v/v), RT, 2hr. Alternatively, coupling of construct (22) (following removal of Fmoc) with an a-bromoacid e.g. (28), provides a versatile intermediate 'Y' substituent hi general formula (I) that may be reacted with many reagents. For example, the a-bromide can be displaced with nucleophiles e.g. alcohols, thiols, carbanions etc, to give ethers (i.e. X = '-', W = 'O', in general formula (I)), thioethers (i.e. X = '-', W = 'S', in general formula (I)). The thioethers may optionally be oxidised to the sulphone (see Scheme 9, i.e. X = '-', W = 'SO2', in general formula (I)) (see Grabowska, U. et al, J. Comb. Chem., 2(5), 475-490, 2000, for an example of bromide displacement and tbioether oxidation on the solid phase). Alternatively, (Scheme 6), treatment of an a-hydroxyacid, exemplified by (25) with trimethylsilylchloride and methanol provides the methyl ester (32). Activation of the free hydroxyl to the chloroformate with phosgene in dichloromethane followed by addition of morpholine, then hydrolysis, provides morpholine-4-carboxylic acid-15-carboxy-2-cyclohexyl ethyl ester (33). Coupling of (33) to the solid phase construct (22) followed by cleavage, provides (34), an example of general formula (I) where R12 = 'H', (X)0 = '-', (W)n = 'O', n = 1, (V)m = 'CO' and U = morpholino. To those skilled in the practices of organic synthesis, a wide variety of a-hydroxyacid esters such as (32) could be converted to the activated chloroformate following the general conditions detailed. Additionally, morpholine may be replaced by any reasonable amine, providing many variations of carboxylic acid (33) following the general conditions detailed. Thus analogues of (34) exploring a wide range of (V)m and U in general formula (I) may be prepared through the general conditions detailed in Scheme 6. Altematively, (Scheme 7), a wide range of alkylsuccinate esters exemplified by 2R-cydohexylmethylsuccinic acid 1-methyl ester (35) are commercially available or readily prepared by known methods (see (a) Azam et al, J. Chem. Soc. Perkin Trans. 1, 621-, 1996; (b) Evans et al, J. Chem. Soc. Perkin Trans. 1, 103, 2127, 1981; (c) Oikawa et al, Tet. Lett, 37, 6169,1996). Carboxyl activation of alkylsuccinate ester (35) followed by addition of morpholine in dimethylformamide and subsequent ester hydroylsis, provides 2/?-cyclohexylmethyl-4-morpholin-4-yl-4-oxo-butyric acid (36). Coupling of (36) to the solid phase construct (22) followed by cleavage, provides (37), an example of general formula (I) where R12 = 'H', (X)0 = 'CH2' i.e. R14, R15 = 'H', o=l, (W)n = 'CO', n = 1, (V)m = '-' and U = morpholino. To those skilled in the practices of organic synthesis, a wide variety of alkylsuccinate esters such as (35) may be prepared and converted to the corresponding substituted alkylsuccinate acid such as (36) following the general conditions detailed. Additionally, morpholine may be replaced by any reasonable amine, providing many variations of carboxylic acid (36) following the general conditions detailed. Thus analogues of (37) exploring a wide range of (X)0, (V)m and U in general formula (I) may be prepared through the general co Scheme 7. (a) LEDC / 1-hydroxybenzotriazole /DMF, 0°C, SOmins. ii. Morpholine, RT, o/n (b) LiOH in H20 / dioxan, 0°C (c) 20% piperidine / DMF, SOmins. (d) lOeq (36) / lOeq HBTU / lOeq HOBt / 20eq NMM, DMF, RT, o/n (e) TFA / H20 (95:5, v/v), RT, 2hr. Alternatively, (Scheme 8), a wide range of biarylalkylacetic acids, exemplified by 2&S-biphenyl-3-yl-4-methylpentanoic acid (39) are readily available by known methods (see (a) DesJarlais, R. L. et al, J. Am. Chem. Soc, 120. 9114-9115,1998; (b) Oballa, R. M. et al, WO 0149288). Coupling of biarylalkylacetic acid (39) to the solid phase construct (22) followed by cleavage, provides (40), an example of general formula (I) where R12 = 'H', (X)0 = '-', (W)n = '-', (V)m = '-' and U = m-biphenyl. To those skilled in the practices of organic synthesis, a wide variety of biarylalkylacetic acids such as (39) may be prepared by alkylation of the a-anion of the free acid analogue of (38), which in turn is prepared by Suzuki coupling of phenylboronic acid and 3-bromophenylacetic acid methyl ester. Phenylboronic acid may be replaced by a wide range of arylboronic acids in the Suzuki coupling, providing many variations of carboxylic acid (39) following the general conditions detailed. Thus analogues of (40) exploring a wide range of group 'U' in general formula (I) may be prepared through the general conditions detailed in Scheme 8. Scheme 8. (a) LiOH in H2O / dioxan, 0°C (b) LLDA, THF, 2-methylpropenylbromide. ii. Pd/C, EtOH, H2 (c) 20% piperidine / DMF, 30mins. (d) lOeq (39) / lOeq HBTU / lOeq HOBt / 20eq NMM, DMF, RT, o/n (e) TFA / H2O (95:5, v/v), RT, 2hr. Many other possibilities for solid phase organic chemistry (e.g. see Brown, R. D. J. Chem. Soc., Perkin Trans. 1, 19, 3293-3320, 1998, for a review of recent SPOC publications) can be used to derivatise construct (22) towards compounds of general formula (I) (and similarly, through the appropriate loaded derivative, compounds of general formula (II)). For example, the left-hand portion 'U-V-W-X-Y1 in general formulae (I) can be partially constructed in solution, coupled to construct (22) and further modified on the solid phase. For example (Scheme 9), a simple extension of Scheme 5 is through the oxidation of the intermediate solid phase bound species, with m-chloroperbenzoic acid in dichloromethane prior to cleavage, to give the sulphone analogue (42). Commencing from a variation of carboxylic acid (30), 3-cyclohexyl-25-(furan-2-ylmethylsulfanyl)propionic acid (41), sulphone (42) is prepared, an example of general formula (I) where R12 = 'H', (X)0 = '-', (W)n = 'S02', n = 1, (V)m = 'CH2', i.e. R17, R18 = 'H', m = 1 and U = 2-furanyL As described in Scheme 5, many variations of carboxylic acid (30) may be prepared following the general conditions detailed e.g. (41). Thus analogues of (42) exploring a wide range of (V)m and U in general formula (I) may be prepared through the general conditions detailed in Schemes 5 and 9. Compounds of general formulae (I) and (II) are finally released from the solid phase by treatment with trifhioroacetic acid / water, followed by evaporation, lyophylis and standard analytical characterisation. A second strategy for the synthesis of compounds of general formulae (I) and (II) comprises:- (a) Preparation of an appropriately functionalised and protected tetrahydrofuro[3,2-6]pyrrol-3-one, tetrahydrothieno[3,2-£]pyrrol-3-one, hexahydropyrrolo[3,2-6] pyrrol-3-one, hexahydrocyclopenta[fc]pyrrol-6-one, tetrahydrofuro[3,2-c]pyrazol-6-one, tetrahydrothienop^-cjpyrazol-e-one, hexahydropyrrolo[3,2-c]pyrazol-6-one, hexahydrocyclopentapyrazol-6-one, hexahydrofuro[3,2-fc]pyridin-3-one, hexahydrothieno[3,2-&]pyridin-3-one, octahydropyrrolo[3,2-6] pyridin-3-one, octahydrocyclopenta[6]pyridin-7-one, hexahydrofuro[3,2-c]pyridazin-7-one, hexahydrothieno[3,2-c]pyridazm-7- one, octahydropyrrolo[3,2-c]pyridazin-7-one or octahydrocyclopenta[c]pyrida2in-7-one building block in solution. Preferred protecting groups for solution phase chemistry are the Na-terf- butoxycarbonyl group and the Na-benzyloxycarbonyl group. (b) Standard organic chemistry methods for the conversion of building block (a) towards compounds of general formulae (I) and (II). In the simplest example, the entire left hand portion of an inhibitor of general formulae (I) and (II) can be prepared in solution by traditional organic chemistry methods and coupled to building block (a) (see Scheme 10 exemplified by preparation and use of the 3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carboxyUc acid tert-butyl ester (45)). cheme 10. (a) 'BuOCOCl, NMM, DCM, -15°C, lOmins, under argon, (b) Diazomethane in diethyl ether, -15°C to RT over 24hr. (c) Acetic acid (d) Lid (lOeq) in 80%aq acetic acid, 5°C to RT over Ihr. (e) 4M HCI in dioxan, 0°C, 2hrs. (f) Pre-prepared U-V-W-X-Y-COOH / activation e.g. HATU / HOAt / NMM, DMF, RT, o/n. The general strategy detailed in Scheme 10 is particularly useful when the compound of general formula (I) contains a substituent that is labile to trifluoroacetic acid, this being the final reagent used in each of the solid phase Schemes 4-9. For example (Scheme 11), treatment in solution of a-hydroxyacid (47) with sodium hydride in a dimethylformamide / dichloromethane mixture followed by addition of 4-tert-butylbenzyl bromide, provides 2J?5'-(4-rer?-butylbenzyloxy)-4-methylpentanoic acid (48). Coupling of (48) to hydrochloride salt (46), provides (49), an example of general formula (I) where R12 = 'H', (X)0 = '-', (W)n = 'O', n = 1, (V)m = 'CH2', i.e. R17, R18 = 'H', m = 1 and U = 4-tert-butylphenyl. To those skilled in the practices of organic synthesis, 4-tert-butylbenzyl bromide may be replaced by any reasonable Ar-CR17R18-halide, providing many variations of carboxylic acid (48) under the conditions shown. Thus analogues of (49) exploring a wide range of (V)m and U in general formula (I) may be prepared through the conditions detailed in Scheme 11. Scheme 11. (a)2.2eq NaH, 1:1 DMF / DCM, 1.25eq 4-tert-benzylbromide, 2hr (b)leq (48), leq teuOCOCl, 2eqNMM, DCM, -15°C, Ihr, under nitrogen, then leq, (46), RT, o/n. A third strategy for the synthesis of compounds of general formulae (I) and (II) where the addition of U-V-W-X-Y to the protected building block involves multistep organic reactions comprises:- (a) Preparation of an appropriately functionalised and protected tetrahydrofuro[3,2-&]pyrrol-3-one, tetrahydrothieno[3,2-fe]pyrrol-3-one, hexahydropyrrolo[3,2-6]pyrrol-3-one, hexahydrocyclopenta[&]pyrrol-6-one, tetrahydrofuro[3,2-c]pyrazol-6-one, tetrahydrothieno[3,2-c]pyrazol-6-one, hexahyd^opyrrolo[332Tc]pyrazol-6-one, hexahydrocyclopentapyrazol-6-one, hexahydrofuro[3,2-i]pyridin-3-one, hexahydrothieno[3,2-&]pyridin-3-one, octahydropyrrolo[3,2-i] pyridin-3-one, octahydrocyclopenta[i]pyridin-7-one, hexahydrofuro[3j2-c]pyridazin-7-one., hexahydrothieno[3,2-c]pyridazin-7- one, octahydropyrroloCS^-clpyridazin^-one or octahydrocyclopenta[c] pyridazin-7-one building block in solution. Preferred protecting groups for solution phase chemistry are the Na,-tert-butoxycarbonyl group and the Na-benzyloxycarbonyl group. (b) Protection of the ketone functionality of the tetrahydrofuro[3,2-6]pyrrol-3- one, tetrahydrothieno[3,2-6]pyrrol-3-one, hexahydropyrrolo[332-6]pyrrol-3- one, hexahydrocyclopenta[6]pyrrol-6-one3 tetrahydrofuro[3,2-c]pyrazol-6- one, tetrahydrothieno[3,2-c]pyrazol-6-one, hexahydropyrrolo[3,2-c]pyrazol-6-one, hexahydrocyclopentapyrazol-6-one, hexahydrofuro[3,2-&]pyridin-3-one, hexahydrothieno[3,2-&]pyridin-3-one, octahydropyrrolo[3,2-&]pyridin-3-one, octahydrocyclopenta[6]pyridin-7-one, hexahydrofuro[3,2-c]pyridazin-7-one, hexaliydrothieno[3,2-c]pyridazin-7-one, octahydropyrrolo[3,2-c]pyridazin-7-one or octahydrocyclopenta[c]pyridazin-7-one building block e.g. as a dimethylacetal. Alternatively, the ketone may be reduced to the achiral secondary alcohols and re-oxidised as the final synthetic step. (c) Standard organic chemistry methods for the conversion of building block (b) towards compounds of general formulae (I) and (II). (d) Intermediates may be prepared in solution, followed by coupling to building block (b) and further derivitisation towards compounds of general formulae (I) and (II) (see Scheme 12 exemplified by preparation and use of the 3- hydroxyhexahydrofuro[3,2-6]pyrrole-4-carboxylic acid tert-butyl ester (50)). Alternatively, depending upon the types of chemistry used to construct the left hand side U-V-W-X-Y of compounds of general formula (I) and (II), the ketone may require protection e.g. as the dimethyl acetal. Such a method is detailed and exemplified in Scheme 13 by the preparation and use of 3,3-dimethoxyhexahydrofur Scheme 13. (a) Triethylorthofonnate / pTSA / MeOH. (b) H2, Pd-C. (c) Stepwise reaction with intennediates of Y, then X, then W etc., to stepwise construct compounds (56). (d) Trifluoroacetic acid/CH2Cl2/H20. The invention extends to novel intermediates as described above, and to processes for preparing compounds of general formulae (I) or (II) from each of their immediate precursors. In turn, processes for preparing intermediates from their immediate precursors also form part of the invention. Compounds of general formulae (I) and (II) are useful both as laboratory tools and as ^ therapeutic agents. In the laboratory certain compounds of the invention are useful in establishing whether a known or newly discovered cysteine protease contributes a critical or at least significant biochemical function during the establishment or progression of a disease state, a process commonly referred to as 'target validation' . According to a second aspect of the invention, there is provided a method of validating a known or putative cysteine protease inhibitor as a therapeutic target, the method comprising: (a) assessing the in vitro binding of a compound as described above to an isolated known or putative cysteine protease, providing a measure of potency; and optionally, one or more of the steps of: (b) assessing the binding of the compound to closely related homologous proteases of the target and general house-keeping proteases (e.g. trypsin) to provides a measure of selectivity; (c) monitoring a cell-based functional marker of a particular cysteine protease activity, in the presence of the compound; and (d) monitoring an animal model-based functional marker of a particular cysteine protease activity in the presence of the compound. The invention therefore provides a method of validating a known or putative cysteine protease inhibitor as a therapeutic target. Differing approaches and levels of complexity are appropriate to the effective inhibition and 'validation' of a particular target. In the first instance, the method comprises assessing the in viti'o binding of a compound of general formulae (I) or (II) to an isolated known or putative cysteine protease, providing a measure of 'potency'. An additional assessment of the binding of a compound of general formulae (I) or (IT) to closely related homologous proteases of the target and general house-keeping proteases (e.g. trypsin) provides a measure of 'selectivity'. A second level of complexity may be assessed by monitoring a cell-based functional marker of a particular cysteine protease activity, in the presence of a compound of general formulae (I) or (II). For example, a 'human osteoclast resorption assay' has been utilised as a cell-based secondary in vifro testing system for monitoring the activity of cathepsin K and the biochemical effect of protease inhibitors (e.g. see WO-A-9850533). An 'MHC-II processing - T-cell -119- activation assay' has been utilised as a cell-based secondary in vitro testing system for monitoring the activity of cathepsin S and the biochemical effect of protease inhibitors (Shi, G-P., et at, Immunity, 10, 197-206,1999). When investigating viral or bacterial infections such a marker could simply be a functional assessment of viral (e.g. count of mRNA copies) or bacterial loading and assessing the biochemical effect of protease inhibitors. A third level of complexity may be assessed by monitoring an animal model-based functional marker of a particular cysteine protease activity, in the presence of a compound of general formulae (I) or (II). For example, murine models of Leishmania infection, P. vinckei infection, malaria (inhibition of falcipain) and T. cnizi infection (cruzipain), indicate that inhibition of cysteine proteases that play a key role in pathogen propagation is effective in arresting disease symptoms, 'validating' said targets. The invention therefore extends to the use of a compound of general formulae (I) or (H) in the validation of a known or putative cysteine protease inhibitor as a therapeutic target. Compounds of general formulae (I) and (II) are useful for the in vivo treatment or prevention of diseases in which participation of a cysteine protease is implicated. According to a third aspect of the invention, there is provided a compound of general formulae (I) or (II) for use in medicine, especially for preventing or treating diseases in which the disease pathology may be modified by inhibiting a cysteine protease. According to a fourth aspect of the invention, there is provided the use of a compound of general formulae (I) or (II) in the preparation of a medicament for preventing or treating diseases in which the disease pathology may be modified by inhibiting a cysteine protease. Certain cysteine proteases function in the normal physiological process of protein degradation in animals, including humans, e.g. in the degradation of connective tissue. However, elevated levels of these eirzymes in the body can result in jathological conditions leading to disease. Thus, cysteine proteases have been mplicated in various disease states, including but not limited to, infections by °neumocystis carinii, Trypsanoma cruzi, Trypsanoma brucei brucei and Criihidia The invention is useful in the prevention and/or treatment of each of the disease states mentioned or implied above. The present invention also is useful in a methods of treatment or prevention of diseases caused by pathological levels of cysteine proteases, particularly cysteine proteases of the papain superfamily, which methods comprise administering to an animal, particularly a mammal, most particularly a human, in need thereof a compound of the present invention. The present invention particularly provides methods for treating diseases in which cysteine proteases are implicated, including infections by Pneumocystis carinii, Trypsanoma cntzi, Trypsanoma brucei, Leishmania mexicana, Clostridium histofyticum, Staphylococcus aureus, foot-and-mouth disease virus and Criihidia fusiculata; as well as in osteoporosis, autoimmunity, schistosomiasis, malaria, tumour metastasis, metachromatic leukodystrophy, muscular dystrophy and amytrophy. Inhibitors of cruzipain, particularly cruzipain-specific compounds, are useful for the treatment of Chagas' disease. In accordance with this invention, an effective amount of a compound of general formulae (I) or (H) may be administered to inhibit the protease implicated with a particular condition or disease. Of course, this dosage amount will further be modified according to the type of administration of the compound. For example, to achieve an "effective amount" for acute therapy, parenteral administration of a compound of general formulae (I) or (II) is preferred. An intravenous infusion of the compound in 5% dextrose in water or normal saline, or a similar formulation with suitable excipients, is most effective, although an intramuscular bolus injection is also useful. Typically, the parenteral dose will be about 0.01 to about 100 mg/kg; preferably between 0.1 and 20 mg/kg, in a manner to maintain the concentration of drug in the plasma at a concentration effective to inhibit a cysteine protease. The compounds may be administered one to four times daily at a level to achieve a total daily dose of about 0.4 to about 400 mg/kg/day. The precise amount of an inventive -compound which is therapeutically effective, and the route by which such compound is best administered, is readily determined by one of ordinary skill in the art by comparing the blood level of the agent to the concentration required to have a therapeutic effect. Prodrugs of compounds of the present invention may be prepared by any suitable method. For those compounds in which the prodrug moiety is. a .ketone functionality, specifically ketals and/or hemiacetals, the conversion may be effected in accordance with conventional methods. The compounds of this invention may also be administered orally to the patient, in a manner such that the concentration of drug is sufficient to inhibit bone resorption or to achieve any other therapeutic indication as disclosed herein. Typically, a pharmaceutical composition containing the compound is administered at an oral dose of between about 0.1 to about 50 mg/kg in a manner consistent with the condition of the patient. Preferably the oral dose would be about 0.5 to about 20 mg/kg. No unacceptable toxicological effects are expected when compounds of the present invention are administered in accordance with the present invention. The compounds of this invention, which may have good bioavailability, may be tested in one of several biological assays to determine the concentration of a compound which is required to have a given pharmacological effect. According to a fifth aspect of the invention, there is provided a pharmaceutical or veterinary composition comprising one or more compounds of general formulae (I) or (II) and a pharmaceutically or veterinarily acceptable carrier. Other active materials may also be present, as may be considered appropriate or advisable for the disease or condition being treated or prevented. The carrier, or, if more than one be present, each of the carriers, must be acceptable in the sense of being compatible with the other ingredients of the formulation and not deleterious to the recipient. The formulations include those suitable for rectal, nasal, topical (including buccal and sublingual), vaginal or parenteral (including subcutaneous, intramuscular, intravenous and intradermal) administration, but preferably the formulation is an orally administered formulation. The formulations may conveniently be presented in unit dosage form, e.g. tablets and sustained release capsules, and may be prepared by any methods well known in the art of pharmacy. Such methods include the step of bringing into association the above defined active agent with the carrier. In general, the formulations are prepared by uniformly and intimately bringing into association the active agent with liquid carriers or finely divided solid carriers or both, and then if necessary shaping the product. The invention extends to methods for preparing a pharmaceutical composition comprising bringing a compound of general formulae (I) or (II) in conjunction or association with a pharmaceutically or veterinarily acceptable carrier or vehicle. Formulations for oral administration in the present invention may be presented as: discrete units such as capsules, cachets or tablets each containing a predetermined amount of the active agent; as a powder or granules; as a solution or a suspension of the active agent in an aqueous liquid or a non-aqueous liquid; or as an oil-in-water liquid emulsion or a water in oil liquid emulsion; or as a bolus etc. For compositions for oral administration (e.g. tablets and capsules), the term "acceptable carrier" includes vehicles such as common excipients e.g. binding agents, for example syrup, acacia, gelatin, sorbitol, tragacanth, polyvinylpyrrolidone (Povidone), methylcellulose, ethylcellulose, sodium carboxymethylcellulose, hydroxypropylmethylcellulose, sucrose and starch; fillers and carriers, for example com starch, gelatin, lactose, sucrose, microcrystalline cellulose, kaolin, mannitol, dicalcium phosphate, sodium chloride and alginic acid; and lubricants such as magnesium stearate, sodium stearate and other metallic stearates, glycerol stearate stearic acid, silicone fluid, talc waxes, oils and colloidal silica. Flavouring agents such as peppermint, oil of wintergreen, cherry flavouring and the like can also be used. It may be desirable to add a colouring agent to make the dosage form readily identifiable. Tablets may also be coated by methods well known in the art. A tablet may be made by compression or moulding, optionally with one or more accessory ingredients. Compressed tablets may be prepared by compressing in a suitable machine the active agent in a free flowing form such as a powder or granules, optionally mixed with a binder, lubricant, inert diluent, preservative, surface-active or dispersing agent. Moulded tablets may be made by moulding in a suitable machine a mixture of the powdered compound moistened with an inert liquid diluent. The tablets may be optionally be coated or scored and may be formulated so as to provide slow or controlled release of the active agent. Other formulations suitable for oral administration include lozenges comprising the active agent hi a flavoured base, usually sucrose and acacia or tragacanth; pastilles comprising the active agent in an inert base such as gelatin and glycerin, or sucrose and acacia; and mouthwashes comprising the active agent in a suitable liquid carrier. Parenteral formulations will generally be sterile. According to a sixth aspect of the invention, there is provided a process for the preparation of a pharmaceutical or veterinary composition as described above, the process comprising bringing the active compound(s) into association with the carrier, for example by admixture. Preferred features for each aspect of the invention are as for each other aspect mutatis mutandis. The invention will now be illustrated with the following examples: Solution Phase Chemistry - General Methods All solvents were purchased from ROMIL Ltd (Waterbeach, Cambridge, UK) at SpS or Hi-Dry grade unless otherwise stated. General peptide synthesis reagents were obtained from Chem-Impex Intl. Inc. (Wood Dale IL 60191. USA). Thin layer chromatography (TLC) was performed on pre-coated plates (Merck aluminium sheets silica 60 F254, part no. 5554). Visualisation of compounds was achieved under ultraviolet light (254nm) or by using an appropriate staining reagent. Flash column purification was performed on silica gel 60 (Merck 9385). All analytical HPLC were obtained on Phenomenex Jupiter C4, 5ji, 300A, 250 x 4.6mm, using mixtures of solvent A = 0.1 %aq trifluoroacetic acid (TFA) and solvent B = 90% acetonitrile /10% solvent A on automated Agilent systems with 215 and / or 254nm UV detection. Unless otherwise stated a gradient of 10 - 90% B in A over 25 minutes at 1.5mL / min was performed for full analytical HPLC analysis. HPLC-MS analysis was performed on an Agilent 1100 series LC/MSD, using automated Agilent HPLC systems, with a gradient of 10 - 90% B in A over 10 minutes on Phenomenex Columbus Cg, 5\|i, 300A, 50 x 2.0mm at 0.4mL / min. Nuclear magnetic resonance (NMR) were obtained on a Bruker DPX400 (400MHz 1H frequency; QXI probe) in the solvents and temperature indicated. Chemical shifts are expressed in parts per million (5) and are referenced to residual signals of the solvent. Coupling constants (J) are expressed in Hz. Solid Phase Chemistry - General Methods Example inhibitors (1-156) were prepared through a combination of solution and solid phase Fmoc-based chemistries (see 'Solid Phase Peptide Synthesis', Atherton, E. and Sheppard, R. C., IRL Press Ltd, Oxford, UK, 1989, for a general description). An appropriately protected and functionalised building block was prepared in solution (e.g. general compound (19), Scheme 1), then reversibly attached to the solid phase through an appropriate linker. Rounds of coupling / deprotection / chemical modification e.g. oxidation were then performed until the full length desired molecule was complete (Scheme 2). Example inhibitors (1-156) were then released (cleaved) from the solid phase, analysed, purified and assayed for inhibition verses a range of proteases. Generally, multipins (polyamide 1.2 -> lOjimole loadings, see www.mimotopes.com) were used for the solid phase synthesis, although any suitable solid phase surface could be chosen. In general, the 1.2\|imole gears were used to provide small scale crude examples for preliminary screening, whilst the 10 ^mole crowns were used for scale-up synthesis and purification of preferred examples. Standard coupling and Fmoc deprotection methods were employed (see Grabowska, U. et al, J. Comb. Chem. 2(5), 475-490, 2000. for a thorough description of solid phase multipin methodologies). Preparation of Initial Assembly Building Block-linker constructs (e.g.(21), typically lOOmg to 2g) were carboxyl activated with 2-(lH-benzotriazole-l-yl)-l,l,3,3-tetramethyluronium hexafluoro phosphate (HBTU, 1 mole equivalent), 1-hydroxybenzotriazole.hydrate (HOST, 1 mole equivalent) and N-methyhnorpholine (NMM, 2 mole equivalents) in dimethylformamide (DMF, typically 1 to lOmL) for 5 minutes. Ammo functionalised DA/MDA crowns or HEMA gears (lOjjmole per crown / 1.2umole per gear, 0.33 mole equivalent of total surface amino functionalisation compared to activated construct) were added, followed by additional DMF to cover the solid phase surface. The loading reaction was left overnight. Following overnight loading, crowns / gears were taken through standard cycles washing, Fmoc deprotection and loading quantification (see Grabowska, U. et al) to provide loaded Building Block-linker constructs (e.g.(22)). Analysis indicated virtually quantitative loading in all examples. Coupling Cycles The coupling of standard Fmoc-aminoacids (10 or 20 mole equivalent) were performed via carboxyl activated with 2-(lH4>enzotriazole-l-yl)-l,1,3,3-tetramethyluronium hexafluoro phosphate (HBTU, 10 or 20mole equivalent), 1-hydroxybenzotriazole.hydrate (HOBT, 10 or 20mole equivalent) and N-methylmorpholine (NMM, 20 or 40mole equivalents) in dimethylformamide, with pre-activation for 5 minutes. Activated species were dispensed to the appropriate wells of a polypropylene 96-well plate (Beckman, ImL wells, 500}iL solution per well for crowns or 250fj,L solution per well for gears) in a pattern required for synthesis. Loaded free arnino Building Block-linker constructs (e.g.(22)) were added and the coupling reaction left overnight. Following overnight coupling, crowns / gears were taken through standard cycles washing and Fmoc deprotection (see Grabowska, U. et al). Identical activation and coupling conditions were used for the coupling of a range of carboxylic acids (R-COOH). Alternatively, chloroformates e.g. morpholine-4-carbonylchloride (lOmole equivalent), were coupled in DMF with the addition of NMM (lOmole equivalents). Acidolytic Cleavage Cycle A mixture of 95% TFA / 5% water was pre-dispensed into two polystyrene 96-well plates (Beckman, ImL wells, 600uL solution per well for crowns or 300uL solution per well for gears) in a pattern corresponding to that of the synthesis. The completed multipin assembly was added to the first plate (mother plate), the block covered in tin foil and cleaved for 2 hours. The cleaved multipin assembly was then removed from the first plate and added to the second plate (washing plate) for 15 minutes. The spent multipin assembly was then discarded and the mother / washing plates evaporated on an HT-4 GeneVac plate evaporator. Analysis and Purification of Cleaved Examples (a) Ex 1.2umole Gears. lOOuL dirnethylsulphoxide (DMSO) was added to each post cleaved and dried washing plate well, thoroughly mixed, transferred to the corresponding post cleaved and dried mother plate well and again thoroughly mixed. lOuL of this DMSO solution was diluted to lOOuL with a 90% acetonitrile /10% 0.1 %aq TFA mixture. 20uL aliquots were analysed by HPLC-MS and full analytical HPLC. In each case the crude example molecules gave the expected [M + H]+ ion and an HPLC peak at > 80% (by 215nm UV analysis). This provided an approximately lOmM DMSO stock solution of good quality crude examples for preliminary protease inhibitory screening. (b) Ex lOfimole Crowns. SOOuL of a 90% acetonitrile / 10% 0.1 %aq TFA mixture was added to each washing plate well, thoroughly mixed, transferred to the corresponding mother plate well and again thoroughly mixed. 5uL of this solution was diluted to lOOuL with a 90% acetonitrile / 10% 0.1%aq TFA mixture. 20uL aliquots were analysed by HPLC-MS and full analytical HPLC. In each case the crude example molecules gave the expected [M + H]+ ion and an HPLC peak at > 80% (by 215nm UV analysis). The polystyrene blocks containing crude examples were then lyophilised. (c) Individual examples (ex (b)) were re-dissolved in a 1 :1 mixture of 0.1% aq TFA / acetonitrile (ImL) and purified by semi-preparative HPLC (Phenomenex Jupiter C4, 5\i, 300A, 250 x 10mm, a 25-90% B in A gradient over 25mins, 4.0mL/min, 215nm UV detection). Fractions were lyophilised into pre-tarred glass sample vials to provide purified examples (typically 2 to 4mg, 40 to 80% yield). (d) Purified examples were dissolved hi an appropriate volume of DMSO to provide a lOmM stock solution, for accurate protease inhibitory screening. EXAMPLE 1. (An example of general formula (I)). (3aS, 6aK) N-[\S-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]benzamide Following the general details from Scheme 1, the required bicycle building block (3a5,6aZ?) 3-oxo-hexahydrofuro[3,2-6]pyrrole~4-carboxylic acid 9J?-fluoren-9-yl methyl ester (19) was prepared in 8 steps as follows. (1) Preparation of (2S,3S) (3-hydroxy)pyrrolidine-l,2-dicarboxylic acid 1-(9H-fluoren-9-ylmethyl) ester. Trans-3-hydroxy-L-proline (10.Og, 76.3mmole) was added to a vigorously stirred, ice-cooled solution of sodium carbonate (16.90g, 160.2mmole) in water (lOOmL). 1,4-Dioxan (75mL) was added providing an opaque but mobile mixture. 9-Fluorenylmethyl chloroformate (20.3 Ig, SOmmole) in 1,4-dioxan (75mL) was added over Ihr, then the ice-cooling removed and the mixture stirred at RT for an additional 2hr. Additional water (300mL) was added, the reaction mixture washed with chloroform (2 x 250mL) and the combined organic layers discarded. The aqueous phase was acidified with IN HC1 to ~ pH 2, providing a thick opaque mixture. The acidified aqueous mixture was extracted with chloroform (2 x SOOmL) and the now clear aqueous phase discarded. The opaque combined chloroform layers were dried (Na2SC>4), filtered and reduced in vacuo to provide batch 1 (5.70g). The residual precipitate (a mixture of product and drying agent) was triturated with hot methanol (2 x 250mL) and the combined methanol solutions reduced in vacuo to provide batch 2 (10.25g). Batch 1 and 2 were individually analysed by TLC (single UV spot, Rf = 0.15, 20% MeOH in CHC13), and HPLC-MS (single main UV peak with Rt = 7.069mins, 354.2 [M + H]+, 376.2 [M + Na]+) and found to be identical, giving a combined yield of 15.95g (45.2mmole, 59.2%). Analysis by 1H and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond. 5H (DMSO-d6 at 298K); 1.80-2.02 (2Hy, m), 3.49-3.62 (2H§, rn), 4.12-4.38 (Ha, Hp, Fmoc H-9 and CRg, m), 5.55/5.62 (OH), 7.30-7.31 (2H aromatic, Fmoc H-2 and H-7), 7.35-7.37 (2H aromatic, Fmoc H-3 and H-6), 7.43-7.45 (2H aromatic, Fmoc H-l and H-8), 7.63-7.65 (2H aromatic, Fmoc H-4 and H-5), 12.8-13.0 (COOH). 5C (DMSO-de at 298K); 31.70/32.70 (d, C7), 44.68/45.32 (d, C5), 46.94/46.97 (u, Fmoc C-9), 67.04/67.33 (d, Fmoc CH2), 68.24/68.51 (u, Ca), 73.12/74.23 (u, Cp), 120.49/120.52 (u, Fmoc C-4 and C-5), 125.49/125.58 (u, Fmoc C-l and C-8), 127.50 (u, Fmoc C-2 and C-7), 128.04 (u, Fmoc C-3 and C-6), 140.99/141.09 (q, Fmoc C-4' and C-5'), 144.02/144.16 (q, Fmoc C-l' and C-8'), 154.33/154.54 (q, OCON), 172.10/172.39 (COOH). (2) Preparation of (25,35) (3-hydroxy)pyrrolidine-lJ2-dicarboxylic acid 2-allyl ester l-(9H-fiuoren-9-ylmethyl) ester. (25,35) (3-hydroxy)pyrrolidine-l,2-dicarboxylic acid l-(9H~fluoren-9-ylmethyl) ester (10.9g, 30.8mmole) was dissolved in toluene (75mL) in a Dean-Stark apparatus. Allyl alcohol (20mL) was added followed by p-toluenesulphonic acid.hydrate (6.05g, 31.4mmole). The mixture was refmxed for Ihr, cooled and CHCls (300mL) added. The organic layer was washed with NaHCOs (300mL), 0.1N HC1 (300mL) and brine (300mL), then dried (Na2SO4). Filtration and reduction in vacuo gave a pale yellow foam (13.5g). The crude foam was purified over silica gel (150g) eluting with a gradient of heptane : ethyl acetate 3:1 -> 1:1. Desired fractions were combined and reduced in vacuo to a colourless gum yield 10.34g (26.3mmole, 85.4%). TLC (single UV spot, Rf = 0.30, heptane : ethyl acetate 1:1), analytical HPLC Rt = 18.849mins, HPLC-MS (single mam UV peak with Rt = 8.354mins, 394.2 [M + H]+, 416.2 [M + Naf). Analysis by !H and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond. 5H (CDC13 at 298K); 2.00-2.21 (2HY, m), 2.70/2.85 (OH, b), 3.72-3.81 (2HS, m), 4.12-4.67 (Ha, Hp, Fmoc H-9 and CH^,2 x COOCH2CH=CH2, m), 5.20-5.40 (2 x COOCH2CH=CH2, m), 5.82-5.99 (1 x COOCH2CH=CH2, m), 7.28-7.33 (2H aromatic, Fmoc H-2 and H-7), 7.34-7.41 (2H aromatic, Fmoc H-3 and H-6), 7.53-7.66 (2H aromatic, Fmoc H-l and H-8), 7.77-7.81 (2H aromatic, Fmoc H-4 and H-5). 5C (CDC13 at 298K); 32.28/33.04 (d, C?), 44.98/45.32 (d, C8), 47.56/47.63 (u, Fmoc C-9), 66.44 (d, COOCH2CH=CH2), 68.01/68.11 (d, Fmoc CH2), 68.32/68.72 (u, Ca), 74.49/75.67 (u, Cp), 119.20/119.48 (d,-130- COOCH2CH=CH2), 120.34/120.37 (u, Fmoc C-4 and C-5), 125.36/125.60 (u: Fmoc C-l and C-S), 127.47 (u, Fmoc C-2 and C-7), 128.06/128.12 (u, Fmr -C-3 and C-6), 131.79/131.94 (u, COOCH2CH=CH2), 141.65/141.71 (q, Fmoc C-4' and C-5'), 144.12/144.34 (q, Fmoc C-l' and C-8'), 155.13/155.59 (q, OCON), 170.53/170.55 (COOCH2CH=CH2). (3) Preparation of (2S,3>R) (3-formyloxy)pyrrolidine-l,2-dicarboxylic acid 2- allyl ester l-(9H-fluoren-9-ylmethyl) ester. Triphenylphosphine (2.76g, 10.5mmole, TPP) was dissolved in dry tetrahydrofuran (75mL), under a nitrogen blanket with stirring and ice-cooled. Diisopropylazodicarboxylate (2.12g, 2.07mL, lO.Smmole, DIAD) was added dropwise over ISmins to give a precipitous white mixture. (2S,3S) (3-hydroxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester l-(9H-fluoren-9-ylmethyl) ester (4.15g, lO.Smmole) and formic acid (0.97g, 0.79mL, 2l.0mmole) were mixed in dry tetrahydrofuran (30mL) and added over ISmins to the TPP/DIAD mixture. The reaction was stirred for a further Ihr at 0°C, then overnight at RT. The solvents were removed in vacuo to give a viscous pale yellow oil (ll.lg). The crude oil was purified over silica gel (250g) eluting with a gradient of heptane : ethyl acetate 4:1 -> 1:1. Product fractions were identified and reduced in vacuo to give (2S,3R) (3-formyloxy)pyrrolidine-l32-dicarboxylic acid 2-allyl ester l-(9H-fluoren-9-ylmethyl) ester / Bis-hydrazide of DIAD, a white solid, yield 4.0g. TLC (single UV spot, Rf = 0.50, heptane : ethyl acetate 1:1), analytical HPLC Rt = 19.064mins, HPLC-MS (single main UV peak with Rt = 9.721mins, 422.2 [M + H]+, 444.1 [M + Na]+ and a non-UV peak with Rt = 5.057mins, 205.1 [M + Hf, 431.2 [2M + Naf). (4) Preparation of (2S,3R) (3-hydroxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester l-(9H-fluoren-9-ylmethyl) ester. (2S,3R) (3-formyloxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester 1-(9H-fluoren-9-ylmethyl) ester / Bis-hydrazide of DIAD mixture (4.0g) was dissolved in allyl alcohol (18mL), cH2SO4 (75p.L) added and heated to reflux for 90mins. The mixture was cooled, EtOAc (200mL) added and washed with NaHCO3 (200mL), brine (200mL) and dried (Na2SO4). The solvents were removed in vacuo to give a viscous tan oil (4.2g). The crude oil was purified over silica gel (150g) eluting with a gradient of heptane : ethyl acetate 2:1 -> 1:1. Desired fractions were combined and reduced in vacuo to a colourless gum yield 1.75g (4.44mmole, 42.4%). TLC (single UV spot, Rf = 0.25, heptane : ethyl acetate 1:1), analytical HPLC Rt = 17.795mins, HPLC-MS (single main UV peak with Rt = 8.574mins, 394.2 [M + Hf, 416.2 [M + Na]+). Analysis by !H and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond. 8H (CDC13 at 298K); 2.09-2.19 (2Hy, m), 2.52 (OH, dd, / 1.0, 6.0), 3.54-3.65/3.70-3.82 (2H5, m), 4.14-4.20/4.22-4.30 (Fmoc H-9, dm), 4.30-4.39 (1 x Fmoc CH2, m), 4.43-4.58 (Ha + 1 x Fmoc CH2, m), 4.60-4.65 (Hp, m) 4.65-4.76 (2 x COOCH2CH=CH2, m), 5.23-5.36 (2 x COOCH2CH=CH2, m), 5.81-6.00 (1 x COOCH2CH=CH2, m), 7.28-7.38 (2H aromatic, Fmoc H-2 and H-7), 7.39-7.46 (2H aromatic, Fmoc H-3 and H-6), 7.53-7.69 (2H aromatic, Fmoc H-l and H-8), 7.73-7.81 (2H aromatic, Fmoc H-4 and H-5). 5C (CDC13 at 298K); 32.34/33.22 (d, Cy), 44.65/44.90 (d, C5), 47.57/47.63 (u, Fmoc C-9), 63.89/64.22 (u, Ca), 66.38/66.41 (d, COOCH2CH=CH2), 68.02 (d, Fmoc CH2), 71.84/72.80 (u, Cp), 119.08/119.31 (d, COOCH2CH=CH2), 120.39 (u, Fmoc C-4 and C-5), 125.32/125.46/125.56 (u, Fmoc C-l and C-8), 127.46 (u, Fmoc C-2 and C-7), 128.12 (u, Fmoc C-3 and C-6), 132.03/132.14 (u, COOCH2CH=CH2), 141.65/141.69 (q, Fmoc C-4' and C-5'), 144.00/144.16/144.43/144.48 (q, Fmoc C-l' and C-8'), 154.88/155.25 (q, OCON), 169.94/169.99 (COOCH2CH=CH2). (5) Preparation of (2S,2>R) (3-fe7^butoxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester 1 -(9H-fluoren-9-ylmethyl) ester. (2S,3K) (3-hydroxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester 1-(9H-fluoren-9-ylmethyl) ester (1.75g, 4.45mmole) was dissolved in dry dichloromethane (20mL) in a 50mL glass pressure tube and cooled to -78°C. Isobutylene gas (~10mL) was condensed into the solution and cH2SO4 (lOOuL) added. A stirrer bar was added, the tube was sealed, the cooling removed and stirred at RT for 72hr. The sealed tube was cooled to -78°C, N-methylmorpholine (200\|iL, leq w.r.t. cHaSC^) and allowed to warm to RT, unsealed, with stirring over 2hr. Dichloromethane (75mL) was added and the organics washed with NaHCO3 (75mL), then brine (75mL) and dried (Na2SO4). The solvents were removed in vacuo to give a mobile pale tan oil (2.41 g). The crude oil was purified over silica gel (150g) eluting with a gradient of heptane : ethyl acetate 4:1 -> 3:1. Desired fractions were combined and reduced in vacuo to a thick clear oil yield 1.66g (3.69mmole, 83.0%). TLC (single UV spot, Rf = 0.45, heptane : ethyl acetate 2:1), analytical HPLC Rt = 22.81 Imins, HPLC-MS (single main UV peak with Rt = 11.193mins, 450.2 [M + H]+, 472.2 [M + Naf, 921.4 [2M + Naf). (6) Preparation of (2S,3>K) (3-te^butoxy)pyrrolidine-l,2-dicarboxylic acid 1-(9H-fluoren-9-ylmethyl) ester. (2S,3R) (3-/er/-butoxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester 1-(9H-fluoren-9-ylmethyl) ester (1.60g, 3.56mmole) was dissolved in dry dichloromethane (20mL) with stirring. Tetrakistriphenylphosphine palladium (0) (83mg, 0.071mmole, 0.02eq) was added, followed by phenyltrihydrosilane (0.77g, 0.674mL, 7.12mmole, 2eq). After 2hr, dichloromethane (150mL) was added and the organics washed with 0.01N HC1 (150mL), brine (150mL) and dried (Na2SO4). The solvents were removed in vacuo to give a dark grey solid (2.14g). The crude solid was purified over silica gel (75g) eluting with a gradient of heptane : ethyl acetate 2:1 -^ 1:6. Desired fractions were combined and reduced in vacuo to a white crystalline yield 0.89g (2.17mmole, 61.1%). TLC (single UV spot, Rf = 0.30, heptane : ethyl acetate 1:2), analytical HPLC Rt = 19.135mins, HPLC-MS (single main UV peak with Rt = 9.386mins, 354.1 [M + H-Bu'f, 410.2 [M + H]+, 432.1 [M + Naf, 841.1 [2M + Naf). Analysis by 'H and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond. 8H (CDC13 at 298K); 1.23 (9H, s), 1.98-2.28 (2HY, m), 3.40-3.50 (1H6, m), 3.68-3.81 (IHfi, m), 4.10-4.50 (Ha + Hp + Fmoc H-9 + 2 x Fmoc CH2, m), 7.29-7.40 (2H aromatic, Fmoc H-2 and H-7), 7.42-7.47 (2H aromatic, Fmoc H-3 and H-6), 7.55-7.70 (2H aromatic, Fmoc H-1 and H-8), 7.73-7.85 (2H aromatic, Fmoc H-4 and H-5), 6.30-8.30 (COOH, b). 5C (CDC13 at 298K); 28.285 (u, (CH3)C), 31.47/32.29 (d, CJ, 44.13/44.38 (d, C5), 47.51/47.60 (u, Fmoc C-9), 62.73/62.95 (u, Ca), 67.96/68.13 (d, Fmoc CH2), 71.77/72.67 (u, Cp), 120.33 (u, Fmoc C-4 and C-5), 125.39 /125.50/125.55/125.61 (u, Fmoc C-l and C-8), 127.46 (u, Fmoc C-2 and C-7), 128.01/128.07 (u, Fmoc C-3 and C-6), 141.58/141.66 (q, Fmoc C-4' and C-5'), 144.04/144.23/144.46 (q, Fmoc C-l' and C-8'), 154.95/155.32 (q, OCON), 175.19/175.65 (COOH). (7) Preparation of (2S,3K) (3-te7^butoxy)-2-(2-diazoacetyl)pyrrolidine-l-carboxylic acid 9#-fluoren-9-ylmethyl ester. (2S,3R) (3-ter^butoxy)pyrrolidine-ls2-dicarboxylic acid l-(9H-fluoren-9-ylmethyl) ester (615mg, 1.5mmole) was dissolved with stirring in dry dichloromethane (15mL). The reaction was flushed with nitrogen and cooled to -15°C. Isobutylchloroformate (225mg, 1.6mmole in dry dichloromethane (2.5mL) and N-methylmorpholine (303mg, 3.0mmole in dry dichloromethane (2.5mL) were added simultaneously in 0.5mL aliquots over ISmins. Etheral diazomethane (generated from diazald (4.7g, ~15mmole in diethyl ether (75mL)) onto sodium hydroxide (5.25g) in water (7.5mL) / ethanol (15mL) at 60°C). was added to the activated aminoacid solution and stirred at RT for 24hr. Acetic acid (~2mL) was added to quench the reaction, then tert-butylmemylether (150mL) was added, the organics washed with water (3 x 200mL), then dried (Na2SO4). The solvents were removed in vacuo to give a thick yellow oil (890g). The crude oil was purified over silica gel (75g) eluting with a gradient of heptane : ethyl acetate 3:1 -> 2:1. Desired fractions were combined and reduced in vacuo to give (2S,3R) (3-fe;t-butoxy)-2-(2-diazoacetyl)pyrrolidine-l-carboxylic acid 9#-fiuoren-9-ylrnethyl ester, a pale yellow solid, yield 270mg (0.624mmole, 41.6%). TLC (single UV spot, Rf= 0.55, heptane : ethyl acetate 1:1), analytical HPLC Rt = 20.085mins, HPLC-MS (2 main UV peaks with Rt = 7.48mins, 350.1 [M + H]+, 372.1 [M + Naf, 721.0 [2M + Na]+ and Rt = 9.949mins, 406.2 [M + H-N2f, 456.2 [M + Naf, 889.3 [2M + Naf). Note that upon HPLC-MS analysis, m/z 350.1 corresponds to the desired bicycle product (3aS, 6aR) 3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carboxylic acid 9#-fluoren-9-ylmethyl ester (19). (8) Cyclisation to (3aS, 6aK) 3-oxo-hexahydrofuro[3,2-&]pyrrole-4-carboxylic acid 9#-fluoren-9-ylmethyl ester (19). A solution of lithium chloride (205mg, 4.8mmole) in water (1.25mL) and acetic acid (5.0mL) was added to (2S,3£) (3-te/-butoxy)-2-(2-diazoacetyl)pyrrolidine-l-carboxylic acid 9H-fluoren-9-ylmethyl ester (209mg, 0.483mmole). Gas was evolved and the yellow oily solid dissolved over Ihr to give a virtually colourless solution. After 90mins, chloroform (150mL) was added and the organics washed with NaHCOa (2 x ISOmL), brine (ISOmL) and dried (Na2SC>4). The solvents were removed in vacuo to give a thick yellow gum (200mg). The crude gum was purified over silica gel (40g) eluting with a gradient of heptane : ethyl acetate 3:2 -> 1:1. Desired fractions were combined and reduced in vacuo to a white crystalline solid, yield 133mg (0.38mmole, 18.6% from starting acid). TLC (single UV spot, Rf = 0.25, heptane : ethyl acetate 1:1), analytical HPLC broad peak Rt = 15.2-17.6mins, HPLC-MS (single broad UV peak with Rt = 7.45-8.56mins, 350.1 [M + Hf, 372.1 [M + Naf, 721.2 [2M + Naf). Analysis by 1H and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond. 5H (CDC13 at 298K); 1.61-1.97 (2Hy, m), 3.32-3.45 (1H8, m), 3.66-3.75/3.85-3.95 (1/2H5+ 1/2H8, dm), 3.95/4.10 (1 x COCH2a + 1 x COCH2p, dm), 4.15-4.30 (Fmoc H-9 + 2 x Fmoc CH2, m), 4.40-4.60 / 4.80-4.92 (Ha + Hp, complex), 7.20-7.30 (2H aromatic, Fmoc H-2 and H-7), 7.31-7.42 (2H aromatic, Fmoc H-3 and H-6), 7.50-7.57/7.60-7.66 (2H aromatic, Fmoc H-l and H-8), 7.68-7.76 (2H aromatic, Fmoc H-4 and H-5). 32.28 (d, Cy), 45.59/45.95 (d, Cs), 47.64 (u, Fmoc C-9), 62.26/62.77 (u, Ca), 68.03/68.65 (d, Fmoc CH2), 71.28 (d, COCH2), 82.17/83.11 (u, Cp), 120.38 (u, Fmoc C-4 and C-5), 125.41 /125.59 /125.88 (u, Fmoc C-l and C-8), 127.45/127.49 (u, Fmoc C-2 and C-7), 128.13 (u, Fmoc C-3 and C-6), 141.73 (q, Fmoc C-4' and C-5'), 144.16/144/37/144.88 (q, Fmoc C-l' and C-8'), 155.33 (q, OCON), 209.32 (COCH2). Following the general details from Scheme 2, the required bicycle building block (3a5} 6aK) 3-oxo-hexahydroruro[3,2-6]pyrrole-4-carboxylic acid 9-fiT-fluoren-9-ylmethyl ester (19) was converted to building block-linker construct (21) as follows: (3aS, 6a#) 3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carboxylic acid 9#-fiuoren-9-ylmethyl ester (19) (lOOmg, 0.286rnmole) was dissolved in a mixture of ethanol (S.OmL) and water (0.75mL) containing sodium acetate.trihydrate (59mg, 0.428mmole, l.Seq). 4-[[(hydrazinocarbonyl)amino] methyl]cyclohexanecarboxylic acid, trifluoroacetate (95mg, 0.286mmole, 1 .Oeq, Murphy, A. M. et al, J. Am. Chem. Soc., 114, 3156-3157, 1992) was added and the mixture refluxed for 90mins. Chloroform (50mL) was added and the organics washed with HC1 (50mL, ~ pH3), dried (Na2SO4) and reduced in vacuo to provide crude building block-linker construct (21) as a colourless gum. Yield 172mg, analytical HPLC 2 peaks Rt = 16.477 and 18.512mins (cis / trans geometrical isomers), HPLC-MS (2 x UV peak with Rt = 7.75 and 8.82mins, 547.3 [M + H]+, 569.3 [M + Na]4). Crude (21) was used directly for construct loading. Following the general details from Scheme 2, the required building block-linker construct (21) was attached to the solid phase providing loaded building block-linker construct (22) as follows: Building block-linker construct (21) (0.26mmoles), 2-(lH-benzotriazole-l-yl)-1,1,3,3-tetramethyluroniumhexafluoro phosphate (HBTU, 98.6mg, 0.26mmole), 1-hydroxybenzorriazole.hydrate and (HOBT, 39.8mg, 0.26mmole) were dissolved in dimethylformamide (3mL) and N-methylmorpholine (NMM, 58^L, 0.52mmole) added. After pre-activation for 5 minutes, free arnine gears (72 x 1.2umole) were added, followed by dimethylformamide (5.5mL) and left overnight. The spent coupling solution was then added to free amine crowns (10 x lOumole) and left overnight. Standard washing and analyses indicated quantitative loading. Following the general details from Scheme 2, the required loaded building block-linker construct (22) was elaborated on the solid phase as follows: Loaded construct (22) was elaborated to EXAMPLE 1 (3aS, 6aR) JV-[liS-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide by standard Fmoc deprotection and sequential coupling with Fmoc-Tyr(OBut)-OH then benzoic acid. The crude example was cleaved and analysed (see general techniques). HPLC Rt = 10.15mins (>95%), HPLC-MS 395.1 [M + Hf. The following examples (2 - 82) were prepared as detailed for EXAMPLE 1, coupling with the required reagents to provide the full length molecule. EXAMPLE 2. (2>aS, 6aR) Biphenyl-4-carboxylic acid [15-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide HPLC Rt = 14.94-15.46mins (> 90%), HPLC-MS 471.2 [M + H]+. EXAMPLE 3. (3aS, 6aR) 4-re^-Butyl-JV-[l1Sr-(4-hydroxyben2yl)-2-oxo-2.(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide HPLC Rt = 15.29-15.89mins (> 90%), HPLC-MS 451.3 [M + H]+ EXAMPLE 4. (3aS, 6aR) ^-[15-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyirol-4-yl)ethyl]-5-thiophen-2-ylnicotinamide HPLC Rt = 11.12 mins (> 90%), HPLC-MS 478.2 [M + H]+ EXAMPLE 5. (3a5, 6aR) 2-Pyridin-3-ylthiazole-4-carboxylic acid [IS-^hydroxy ben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide HPLC Rt = 8.58mins (> 90%), HPLC-MS 479.2[M + H]+ EXAMPLE 6. (2>aS, 6aR) JV-[15-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexaliydrofuro [3,2-6]pyrrol-4-yl)ethyl]-4-thiophen-2-ylbenzamide HPLC Rt = 14.77-15.3Imins (> 90%), HPLC-MS 477.2 [M + H]+ EXAMPLE 7. (3aS, 6aR) A'-[15r-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexaliydroftiro [3,2-6]pyrrol-4-yl)ethyl]-4-trifluoromthoxybenzamide EXAMPLE 8. (3oS, 6aK) Biphenyl-4-carboxylic acid [3-methyl-15-(3-oxo-hexahydrofuro[3,2-&]pyrrole-4-carbonyl)butyl]amide EXAMPLE 10. (3aS, 6aK) //-[3-methyl-l1S-(3-oxo-hexahydrofuro[3,2-&]pyrrole-4-rbonyl) butyl]-4-phenoxybenzamide5XAMPLE 11. (3aS, 6aK) JV-[3-methyl-15-(3-oxo-hexahydrofuro[3,2-fe]pyrrole-4-;arbonyl) butyl]-3-pherioxybenzamide HPLC Rt = 17.34-18.52mins (> 90%), HPLC-MS 437.2 [M + Hf EXAMPLE 12. (3aS, 6aR) 4-Bromo-AT-[3-methyl-15-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-cafbonyl) butyljbenzamide HPLC Rt = 14.81mins (> 90%), HPLC-MS 423.2 / 425.2 [M + H]+ EXAMPLE 13. (3aS, 6aR) 3-Bromo-^-[3-methyl-15-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyljbenzamide HPLC Rt = 14.85-16.20mins (> 90%), HPLC-MS 423.2 / 425.2 [M + H]+ EXAMPLE 14. (3aS, 6aR) 4-Isopropyl-A'-[3-methyl-15-(3-oxo-hexahydrofuro[3,2-&]pyrrole-4-carbonyl) butyl]benzamide HPLC Rt = 16.41-17.65mins (> 90%), HPLC-MS 387.2 [M + Hf EXAMPLE 15. (3aS, 6aR) W-[3-methyl-15-(3-oxo-hexahydroforo[3,2-6]pyrrole-4-carbonyl) butyl]-4-vinylbenzamide HPLC Rt = 14.63mins (> 90%), HPLC-MS 371.1 [M + H]+ EXAMPLE 16. (3aS, 6aR) 4-Imidazol-l-yl-A'-[3-methyl-15-(3-oxo-hexahydrofuro [3,2-i]pyrrole-4-carbonyl) butyl]benzatnide HPLC Rt = 9.20mins (> 90%), HPLC-MS 411.2 [M + H]+ EXAMPLE 17. (3oS, 6aR) //-[3-methyl-15-(3-oxo-hexahydrofuro[3,2-i]pyrrole-4-carbonyl)butyl]-4-thiophen-2-ylben2amide HPLC Rt = 16.49-18.34mins (> 90%), HPLC-MS 427.2 [M + H]+ EXAMPLE 18. (3oS, 6aR) JV-[3-methyl-15-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl)butyl]-4-oxazol-5-ylbenzamide HPLC Rt = 12.79mins (> 90%), HPLC-MS 412.2 [M + H]+ EXAMPLE 19. (3aS, 6aR) 2-Phenylthiazole-4-carboxylic acid [3-methyl-15-(3-oxo- hexaliydrofuro[3,2-6]pyrrole-4-carbonyl) butyl] amide HPLC Rt = 16.41-17.80mins (> 90%), HPLC-MS 428.2 [M + H]+ EXAMPLE 20. (3aS, 6aK) 5-Phenylthiophene-2-carboxylic acid [3-methyl-15-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl)butyl]amide HPLC Rt = 16.89mins (> 90%), HPLC-MS 427.2 [M + H]+ EXAMPLE 21. (3>aS, 6aR~) Quinoline-6-carboxylic acid [3-methyl-l,S-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl)butyl]amide HPLC Rt = 9.08mins (> 90%), HPLC-MS 396.2 [M + Hf EXAMPLE 22. (3aS, 6aR) JV-[3-methyl-l1Sr-(3-oxo-hexahydrofiiro[3,2-6]pyrrole-4-carbonyl) butyl]-4-[l ,2,3]thiadiazol- HPLC Rt = 13.41mins (> 90%), HPLC-MS 429.2 [M + H]+ EXAMPLE 23. (3aS, 6aR) ^-[3-methyl-15-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl)butyl]-5-thiophen-2-ylnicotinamide HPLC Rt = 13.24mins (> 90%), HPLC-MS 428.2 [M + H]+ EXAMPLE 24. (3aS, 6aR) 2-Pyridin-3-ylthiazole-4-carboxylic acid [3-methyl-15-(3-oxo-hexahydrofaro[3,2-6]pyrrole-4-carbonyl)butyl]amide (Figure Remove) HPLC Rt = 10.22mins (> 90%), HPLC-MS 429.2 [M + Hf EXAMPLE 25. (3aS, 6aK) JV-tS-methyl-l^-C carbonyl)butyl]-4-trifluoromethoxybenzamide HPLC Rt = 16.90-18.34mins (> 90%), HPLC-MS 429.2 [M + Hf EXAMPLE 26. (3aS, 6aK) 4-fe7t-Butyl-AT-[l1S-(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-b]pyrrol-4-yl)ethyl]benzamide HPLC Rt = 16.14-17.02mins (> 90%), HPLC-MS 451.2 [M + Hf EXAMPLE 27. (3aS, 6aK) JV-[15-(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexaliydrofuro [3r2-6]pyrrol-4-yl)ethyl]-4-thiophen-2-ylbenzamide(Figure Remove) HPLC Rt = 15.31-16.07mins (> 90%), HPLC-MS 477.2 [M + Hf EXAMPLE 28. (3aS, 6aK) 4-te^-Butyl-^-[15-(4-fluorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-b]pyirol-4-yl)ethyl]ben2amide HPLC Rt = 18.37-19.63mins (> 90%), HPLC-MS 453.1 [M + H]+ EXAMPLE 29. (3aS, 6aK) ]V-[l1S-(4-fluorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]-4-thiophen.-2-ylbenzamide HPLC Rt = 17.45-18.59mins (> 90%), HPLC-MS 479.2 [M + Hf EXAMPLE 30. (3aS, 6aR) Morpholine-4-carboxylic acid [lS-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide HPLC Rt = 7.38mins (>95%), HPLC-MS 404.2 [M + H]+. EXAMPLE 31. (3aS, 6aK) Morpholine-4-carboxylic acid [3-methyl-15-(3-oxo-hexa h.ydrofuro[3.,2-6]pyrrole-4-carbonyl)biityl]amide HPLC-MS 354.2 [M + Hf. EXAMPLE 32. (3aS, 6aR) Morpholine-4-carboxylic acid [lS-cyclohexylmethyl-2- oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide HPLC-MS 394.2 [M + Hf. EXAMPLE 33. (3aS, 6aK) Morpholine-4-carboxylic acid [15-benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-/>]pyrrol-4-yl)ethyl]amide HPLC Rt = 10.07mins (>95%), HPLC-MS 388.1 [M + H]+. EXAMPLE 34. (2RS, 3aS, 6aK) 4-(2-Benzyloxy-3-[cyclohexyl]propionoyl)tetra hydrofuro [3,2-£]pyrrol-3-one. HPLC Rt = 17.5-19.7mins (>90%), HPLC-MS 372.4 [M + H]+, 390.3 [M + H + 18]+. (a) Preparation of 3-Cyclohexyl-2S-hydroxypropionic acid (Compound (25) Scheme 4) A solution of sodium nitrite (12.1g, 175mmol) in water (40ml) was added dropwise to a stirred suspension of (S)-a-aminocyclohexanepropionic acid hydrate (5g, 26.5mmol) in 0.5M sulphuric acid (120ml, 60mmol) at 0°C over 1.Shouts. The mixture was allowed to warm to ambient temperature over 20hours. The product was extracted into diethyl ether (2 x 25ml) then the ethereal layers were washed with saturated aqueous sodium chloride solution (2 x 25ml), dried (Na2S04) and the solvents removed in vacua. The residue (5.3g) was recrystallized from diethyl ether (10ml) and heptane (25ml) to give 3-cyclohexyl-2S-hydroxypropionic acid as a white solid, yield 2.4 g, (53%). 5H (400MHz, CDC13 at 298K), 0.89-1.35 (5H, m) and 1.51-1.86 (7H, m) (OCHCH2 and cyclohexyl), 4.32 (1H, OCHCH2, m) (b)Preparation of 2&S'-Benzyloxy-3-cyclohexylpropionic acid (Compound (26) Scheme 4) Sodium hydride (265mg of 60% dispersion in oil, 6.6mmol) was added in two portions to a stirred mixture of S-cyclohexyWS'-hydroxypropionic acid (0.52g, S.Ommol), dimethylformamide (5ml) and dichloromethane (5ml) at 0°C over Sminutes. The mixture was stirred at 0°C for Sminutes then at ambient temperature for 45minutes. Benzyl bromide (0.45ml, 3.8mmol) was added then the mixture stirred for 1 hour before adding dimethylformamide (5ml). After stirring for 4 hours potassium iodide (50mg, 0.3mmol) was added. The mixture was stirred for 20hours then heated at 55°C for Ihour then allowed to cool to ambient temperature and poured into water (15ml). A saturated aqueous sodium chloride solution (5ml) was added then the mixture was extracted with dichloromethane (5ml then 10ml) that was discarded. The aqueous layer was acidified using 1M hydrochloric acid (10 ml) then extracted with dichloromethane (2 x 10ml). The dichloromethane layer was dried (MgSCU) and the solvent removed in vacua. The residue (0.5 5g) was dissolved in dimethylformamide (8ml) then cooled to 0°C before adding sodium hydride (190mg of 60% dispersion in oil, 4.75mmol). The mixture was stirred for 30 minutes then polymer bound isocyanate (380mg, 2mmolNg~1) added. The mixture was stirred for 2 hours at ambient temperature then poured into water (15ml). 1M Hydrochloric acid (10ml) was added then the product was extracted into dichloromethane (2 x 10ml), dried (Na2SC>4) and the solvent removed in vacua. The residue was purified by flash chromatography over silica gel eluting with a gradient of methanol: dichloromethane 0:1 -> 1:20. Appropriate fractions were combined and the solvents removed in vacua to give 2.&Sr-benzyloxy-3-cyclohexylpropionic acid as a colourless oil, yield 41mg (5.2%). HPLC-MS (single main UV peak with Rt = 9.47mins, 261.2 [M - H]", 285.2[M + Naf,547.3[2M + Na]+). 5H (400MHz, CDC13 at 298K), 0.72-1.03 (2H, cyclohexane, m), 1.08-1.38 (3H, cyclohexane, m), 1.45-1.93 (6H + 2Hp, cyclohexane, m), 3.93-4.18 (IHa, OCHCO), 4.35-4.53 (1H, CH2O, d, /= 11.52 Hz), 4.68-4.88 (1H CH2O, d, J= 11.54 Hz), 7.20-7.47 (5H, ArH, m), 9.36 (1H, OH, brs). Compound (26) was coupled under standard conditions to loaded building block-linker construct (22), then cleaved to provide EXAMPLE 34. EXAMPLE 35. (3aS, 6aK) Morpholine-4-carboxylic acid [17?-benzylsulfanylmethyl-2-oxo-2-(3 -oxo-hexahydrofuro[3,2-i]pyrrol-4-yl)ethyl]amide HPLC Rt = 12.15mins (>95%), HPLC-MS 44.1 [M + Hf. EXAMPLE 36. (3aS, 6aR) ^-[3-methyl-15-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyljbenzamide HPLC Rt = 12.43mins (>95%), HPLC-MS 345.2 [M + H]+. HPLC Rt = 15.09mins (>95%), HPLC-MS 425.1 [M + Hf. EXAMPLE 37. (3aS, 6aR) JV-[l^-benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydro furo[3,2-&]pyrrol-4-yl)ethyl]benzamide EXAMPLE 38. (3aS, 6aK) AT-[l,S'-cyclohexylmethyl-2-oxo-2-(3-oxo-hexaliydrofuro [3,2-6]pyrrol-4-yl)ethyl]benzarnide HPLC Rt = 15.63mins (>90%), HPLC-MS 385.3 [M + H]+, 791.3 [2M + Naf. EXAMPLE 39. (3aS, 6aK) N-[lS-benzyl-2-oxo-2-(3-oxo-hexahydrofaro[3,2-6]pyrrol-4-yl)ethyl]benzarnide HPLC Rt = 12.85mins (>90%), HPLC-MS 379.1 [M + H]+. EXAMPLE 40. (3aS, 6aR) Furan-3-carboxylic acid [15-cyclopentylmethyl-2-oxo-2-(3-oxo-hexah.ydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide HPLC Rt = 13.23mins (>95%), HPLC-MS 361.2 [M + H]+. EXAMPLE 41. (3oS, 6aK) 7V-[15-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-6]pyrrol-4-yl)ethyl]-2-phenylacetamide HPLC Rt = lOJlmins (>95%), HPLC-MS 409.1 [M + H]+. EXAMPLE 42. (3aS, 6aR) ^[3-me1hyl-15-(3K>xo-hexahydrofuro[3^-6]pyirole-4-carbonyl) butyl]-2-phenylacetamide HPLC Rt = 13.00mins (>95%), HPLC-MS 359.2 [M + H]+. EXAMPLE 43. (3aS, 6aR) JV-[l/?-benzylsulfanylmethyl-2-oxo-2-(3-oxo-b.exahydro furo[3}2-6]pyrrol-4-yl)ethyl]-2-phenylacetamide (Figure Remove)HPLC Rt = 15.53mins (>90%), HPLC-MS 439.1 [M + H]+. EXAMPLE 44. (3aS, 6aR) J?/-[15r-cyclohexylmethyl-2-oxo-2-(3-oxo-hexahydrofuro [3,2-fe]pyrrol-4-yl)ethyl]-2-phenylacetamide HPLC Rt = 18.5mins (>90%), HPLC-MS 399.2 [M + H]+. EXAMPLE 45. (3aS, 6aK) JV-[lJSr-ben2yl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-2-phenylacetamide HPLC Rt = 13.2mins (>90%), HPLC-MS 393.2 [M + H]+. EXAMPLE 46. (3aS, 6aK) Furan-3-carboxylic acid [16'-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexab.ydrofuro[3,2-Z>]pyrrol-4-yl)ethyl]ainide HPLC Rt = 8.65mins (>95%), HPLC-MS 385.2 [M + Hf. EXAMPLE 47. (3aS, 6aK) Furan-3-carboxylic acid [3-metliyl-15'-(3-oxo-hexahydro ruro[3,2-fo]pyrrole-4-carbonyl)butyl]amide HPLC Rt = 10.95mins (>90%), HPLC-MS 335.1 [M + H]+, 357.1 [M + Naf. EXAMPLE 48. (3aS, 6aR) Furan-3-carboxylic acid [l^-ben2ylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]amide LC Rt = 13.68mins (>90%), HPLC-MS 415.1 [M + Hf. EXAMPLE 49. (3aS, 6aK) Furan-3-carboxylic acid [15'-cycloriexylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide (Figure Remove) HPLC Rt = 14.39mins (>90%), HPLC-MS 375.2 [M + H]+, 771.3 [2M + Na]+. EXAMPLE 50. (3aS, 6aK) Furan-3-carboxylic acid [lS-ben2yl-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide HPLC Rt = 11 .Slmins (>90%), HPLC-MS 369.1 [M + Hf. EXAMPLE 51. (3aS, 6aR) Thiophene-3-carb.oxylic acid [15-cyclopentyhnethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)et;hyl]arnide HPLC Rt = 14.07mins (>95%), HPLC-MS 377.3 [M + Hf. EXAMPLE 52. (3aS, 6aK) Thiophene-3-carboxylic acid [lS-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide HPLC Rt = 11.75mins (>95%), HPLC-MS 401.3 [M + H]+. EXAMPLE 53. (3aS, 6aR) Thiophene-3-carboxylic acid [3-methyl-15-(3-oxo-hexa hydroruro[3,2-fo]pyrrole-4-carbonyl)butyl]amide HPLC Rt = H.Olmins (>90%), HPLC-MS 351.1 [M + H]+, 373.1 [M + Naf. EXAMPLE 54. (3aS, 6aR) Thiophene-3-carboxylic acid [1^-benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide HPLC Rt = 14.51mins (>95%), HPLC-MS 431.1 [M + H]+. EXAMPLE 55. (3aS, 6aR) Thiophene-3-carboxylic acid [15-cyclohexylmethyl-2-oxo-2-(3-oxo-hexahydroforo[3,2-6]pyrrol-4-yl)ethyl]amide (Figure Remove)-157-1 [M + Hf, 803.3 [2M + Naf. EXAMPLE 56. (3aS, 6aK) Thiophene-3-carboxylic acid [l,S-benzyl-2-oxc-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)etb.yl]amide HPLC Rt = 12.27mins (>95%), HPLC-MS 385.1[M + Hf, 407.1 [M + Naf, 791.2 [2M + Naf. EXAMPLE 57. (3aS, 6aK) Furan-3-carboxylic acid [3,3-dimethyl-15-(3-oxo-hexa hydrofuro[3,2-6]pyrrole-4-carbonyl)butyl]amide HPLC Rt = 12.50mins (>95%), HPLC-MS 349.2 [M + H]+. EXAMPLE 58. (3aS, 6aR) JV-[15-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-Z)]pyrrol-4-yl)ethyl]-3-plienylpropionamide HPLC Rt = 11.72mins (>90%), HPLC-MS 423.2 [M + H]+. EXAMPLE 59. (3aS, 6aR) JV-[3-methyl-15r-(3-oxo-hexaliydrofuro[3,2-6]pyrrole-4-carbonyl) butyl] -3 -phenylpropionamide HPLC Rt = 13.46mins (>90%), HPLC-MS 373.1 [M + H]+, 767.3 [2M + Naf. EXAMPLE 60. (3aS, 6aK) JV-[l^-benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydro furo[3,2-6]pyrrol-4-yl)ethyl]-3-phenylpropionainide HPLC Rt = 16.22mins (>90%), HPLC-MS 453.2 [M + H]+, 475.0 [M + Naf. EXAMPLE 61. (3aS, 6aK) JV-[15-cycloliexylmethyl-2-oxo-2-(3-oxo-hexaliydrofuro [3,2-&]pyrrol-4-yl)ethyl]-3 -phenylpropionamide (Figure Remove) HPLC-MS 413.2 [M + Hf. EXAMPLE 62. (3aS, 6aK) ^-[15-benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-b]pyrrol-4-yl)ethyl]-3-phenylpropionamide HPLC Rt = 16.68mins (>90%), HPLC-MS 407.2 [M + Hf, 835.2 [2M + Naf. EXAMPLE 63. (3aS, 6aK) Thiophene-3-carboxylic acid [3,3-dimethyl-l.S-(3-oxo-hexahydrofuro[352-6]pyrrole-4-carbonyl)butyl]amide HPLC Rt = 13.24mins (>95%), HPLC-MS 365.2 [M + Hf. EXAMPLE 64. (3aS, 6aR) Benzo[&]thiophene-2-carboxylic acid [15-(4-hydroxy ben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide HPLC-MS 451.1 [M + Hf, 923.2 [2M + Naf. EXAMPLE 65. (3aS, 6aR) Benzo[6]thiopliene-2-carboxylic acid [3-methyl-l,Sr-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl)butyl]amide HPLC Rt = 15.62mins (>90%), HPLC-MS 401.1 [M + Hf, 823.2 [2M + Naf. EXAMPLE 66. (3aS, 6aR) Benzo[fo]thiophene-2-carboxylic acid [Ifl-beuzylsulfanyl methyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-i]pyrrol-4-yl)etiiyl]amide HPLC Rt = 17.47mins (>90%)5 HPLC-MS 481.1 [M + Hf, 983.2 [2M +Naf. EXAMPLE 67. (3aS, 6aK) Benzo[6]thiopheae-2-carboxylic acid [15-cyclohexyl methyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide HPLC Rt = 18.15mins (>90%), HPLC-MS 441.2 [M + Hf, 903.2 [2M + Naf. EXAMPLE 68. (3aS, 6aR) Benzo[fc]thiophene-2-carboxylic acid [lS-benzyl-2-oxo-2-(3-oxo-hexab.ydrofuro[3,2-fe]pyrrol-4-yl)ethyl]ainide HPLC Rt = 15.58mins (>85%), HPLC-MS 435.1 [M + H]+, 891.2 [2M + Naf. EXAMPLE 69. (3aS, 6aK) Naphthalene-1-carboxylic acid [15'-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)ethyl]amide LC Rt = 12.42mins (>85%), HPLC-MS 445.1 [M + Hf, 911.3 [2M + Naf. EXAMPLE 70. (3aS, 6aR) Naphthalene-1-carboxylic acid [3-methyl-lS-(3-oxo-hexa hydrofuro [3,2-6]pyrrole-4-carbonyl) butyl] amide HPLC Rt = 14.81mins (>S5%), HPLC-MS 395.2 [M + Hf, 811.3 [2M + Na]+. EXAMPLE 71. (3>aS, 6aR) Naphthalene-1-carboxylic acid [IJ^benzylsulfanylmethyl -2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide HPLC Rt = 17.10mins (>90%), HPLC-MS 475.2 [M + H]+. EXAMPLE 72. (3aS, 6aR) Naphthalene-1-carboxylic acid [15-cyclohexyhnethyl-2-oxo-2-(3-oxo-hexah.ydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide HPLC Rt = 17.45mins (>90%), HPLC-MS 435.2 [M + H]+, 891.4 [2M + Naf. EXAMPLE 73. (3aS, 6aR) Naphthalene-1-carboxyHc acid [15-benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-Z?]pyrrol-4-yl)ethyl] amide HPLC Rt = 15.17miBs (>90%), HPLC-MS 429.1 [M + Hf, 879.3 [2M + Na]+. EXAMPLE 74. (IS, 3aS, 6aR) Morpholine-4-carboxylic acid l-cyclohexylmethyl-2-oxo-2-(3-oxo-hexahydromro[3,2-&]pyrrol-4-yl)ethyl ester HPLC-MS 395.2 [M + Hf, 811.4 [2M + Naf. (a) Preparation of 3-cyclohexyl-2S-hydroxypropionic acid methyl ester. (Compound (32), Scheme 6) Trimethylsilyl chloride (1.78ml, 14.0mmol) was added dropwise to a stirred solution of 3-cyclohexyl-2S-hydroxypropionic acid (compound (25) 1.31g, 7.6mmol) in methanol (35ml). The mixture was stirred at room temperature for 20hours then the solvents were removed in vacua to leave a pale yellow oil (1.38g). The crude oil was purified by chromatography over silica gel eluting with a gradient of n-heptane : ethyl acetate (4:1) Appropriate fractions were combined and the solvents removed in vacua to give 3-cyclohexyl-2S-hydroxypropionic acid methyl ester as a colourless oil, yield 1.26 g, (89%). HPLC-MS (single main peak with Rt = 7.43mins, 187.14 [M + Hf, 395.2 [2M +Naf). 6H (400MHz, CDC13 at298K), 0.84-1.04 (2H3 CH2(cyclohexane), m), 1.09-1.36 (3H, CH2(cyclohexane), CH (cyclohexane), m), 1.45-1.77 (7H CH2(cyclohexane), CH(cyclohexane), m), 1.80-1.90 (IHp, m), 2.72 (1H, OH, d, J= 5.99 Hz), 3.80 (3H, CH3O, s), 4.20-4.31 (IHa, m). (b)Preparation of morpholine-4-carboxylic acid l-carboxy-2-cyclohexylethyl ester (Compound (33), Scheme 6) A solution of phosgene (33.6ml, 20% in toluene) was added to 3-cyclohexyl-25r-hydroxypropionic acid methyl ester (compound (32), 0.7 g, 4.03mmol) followed by 6 drops of dimethylformamide. The mixture was stirred at ambient temperature for 16hours then the solvents removed in vacua. The residue was azeotroped with toluene (3 x 20ml), and dissolved in anhydrous dichloromethane (llml). The solution was cooled to 0°C then morpholine (0.86g, 9.85mmol) added. The mixture was stirred for 2 hours then partitioned between dichloromethane (30ml) and 0.5M hydrochloric acid (30ml). The dichloromethane layer was washed with saturated aqueous sodium hydrogen carbonate solution (30ml), saturated aqueous sodium chloride solution (30ml), dried (NaiSC^) and the solvent removed in vacua. The residue was purified by chromatography over silica gel eluting with ethyl acetate : heptane 1:1. Appropriate fractions were combined and the solvents removed in vacua to leave morpholine-4-cafboxylic acid 2-cyclohexyl-l-methoxycarbonylethyl ester (0.13g, 10%) as an oil. A solution of lithium hydroxide monohydrate (17.5mg, 0.418mmol) in water (0.76ml) was added to an iced-water chilled solution of morpholine-4-carboxylic acid 2-cyclohexyl-l-methoxycarbonylethyl ester (llOmg, 0.367mmol) in dioxane (1.5ml). The mixture was stirred at ambient temperature for Ihour then diluted with water (10ml). The aqueous layer was extracted with diethyl ether (2 x 10ml) which was discarded, men acidified to pH = 2 with 6M hydrochloric acid. The product was extracted into diethyl ether (2 x 10ml), then the combined ethereal layers washed with saturated aqueous sodium chloride (10ml), dried (MgS04) and the solvent removed in vacua to give morpholine-4-carboxylic acid 1-carboxy-25-cyclohexylethyl ester as a white solid, yield O.llg, (100% from ester). TLC (single spot, Rf = 0.20, methanol : dichloromethane 1:9), HPLC-MS (single main peak with Rt = 7.614mins, 286.2 [M + Hf, 287.2 [M + 2Hf, 593.3 [2M + Naf). H (400MHz, CDC13 at 298K), 0.75-1.00 (2H, CH2(cyclohexane), m), 1.02-1.28 (4H, CH2(cyclohexane),m), 1.33-1.46 (1H, CH(cyclohexane), m), 1.50-1.79 (6H, CH2(cyclohexane), m), 3.28-3.73 (8H, CH2OCH2 and CH2NCH2 ,m), 4.92-5.02 (lHa,m),5.99(lH,OH,brs). Compound (33) was coupled under standard conditions to loaded building block-linker construct (22), then cleaved to provide EXAMPLE 74. EXAMPLE 75. (3aS, 6aR) 4-[2-(4-fer^-butylbenzylsulfanyl)-4-methylpentanoyl] tetrahydrofuro[3,2-6]pyrrol-3-one HPLC Rt = 21.3-23.4mins (>80%), HPLC-MS 404.4 [M + H]+. (a) Preparation of 2/2-Bromo-4-methylpentanoic acid (Compound (28), Scheme 5) A solution of sodium nitrite (S.lg, 73mmol) in water (15ml) was added drop-wise at 0°C over Shours to a stirred mixture of D-leucine (8.75g, 67mmol), potassium bromide (29.75g, 0.25mol) and concentrated sulphuric acid (8.6ml) in water (100ml). The mixture was stirred for 30 minutes at 0°C then at ambient temperature for 20hours. The product was extracted into diethyl ether (2 x 150ml) then the combined ethereal layers were washed with saturated aqueous sodium chloride solution (2 x 100ml), dried (MgSO4) and the solvent removed in vacuo. The residue was purified by flash chromatography over silica gel eluting with a gradient of methanol : dichloromethane 1:50 -> 1:20. Appropriate fractions were combined and the solvents removed in vacuo to leave 2/?-bromo-4-methylpentanoic acid (28) as a colourless oil, le spot, Rf= 0.2, methanol: dichloromethane 1:20). Additionally, a second crop (5.2g, 40%) of slightly impure product was obtained 5H (400MHz, CDC13 at 298K), 0.95 and 0.99 (both 3H, CH3CH, d, J = 6.55Hz). 1.77-1.89 (1H, CH3CH, m), 1.93 (2Hp, m), 4.31 (IHa, t, J= 7.7Hz), 9.3 (1H, CO2H, brs). (b)Preparation of 2,S-(4-tert-butylbenzylsulfanyl)-4-methylpentanoic acid (Compound (30), Scheme 5) A solution of 2J?-bromo-4-methylpentanoic acid (compound (28), l.lg, 5.6mmol) and (4-(fert-butyl)phenyl)methanethiol (l.Og, 5.6mmol) in dimethylformamide (15ml) was purged with nitrogen for 5 minutes then cooled to 0°C. Triethylamine (0.79ml, 5.7mmol) was added drop-wise over 1 minute then the mixture was stirred for two days at ambient temperature. The solvents were removed in vacua and residue purified by flash chromatography over silica gel eluting with a gradient of methanol : dichloromethane 0:1 -> 1:20. Appropriate fractions were combined and the solvents removed in vacua to leave a residue which was purified by flash chromatography over silica gel eluting with ethyl acetate : heptane 2:5. Appropriate fractions were combined and the solvents removed in vacua to give 2S-(4-tert-butylbenzylsulfanyl)-4-methylpentanoic acid (30) as a colourless oil, yield 150 mg, (9%). TLC (single spot, Rf = 0.2, heptane : ethyl acetate 5:2), analytical HPLC with main peak Rt = 22.117mins, HPLC-MS (main UV peak with Rt = 11.072mins, 317.2 [M + Naf). oH (400MHz, CDC13 at 298K), 0.70 and 0.85 (both 3H, CH3CH, d, / = 6.3), 1.29 (9H, (CH3)3C, s), 1.44-1.51 (1H, CH3CH, m), 1.62-1.75 (2H(3, m), 3.15-3.20 (IHa, m), 3.81 and 3.88 (both 1H, SCH2, d, J- 13.2Hz), 7.25-7.35 (4H, aromatic). Compound (30) was coupled under standard conditions to loaded building block-linker construct (22), then cleaved to provide EXAMPLE 75. EXAMPLE 76. (3aS, 6aK) 4-[3-Cyclohexyl-2S-(ruran-2-ylmethanesulphonyl) propionyl]tetrahy drofuro[3,2-6]pyrrol-3 -one HPLC Rt = 12.8 - IS.lmins (>90%), HPLC-MS 410.1 [M + Hf, 841.2 [2M + Naf. (a)Preparation of (J?)-a-bromocyclohexanepropionic acid A solution of sodium nitrite (0.45g, 6.5mmol) in water (1.2ml) was added dropwise at 0°C over 4 hours to a stirred mixture of (R)-a-aminocyclohexanepropionic acid (Ig, S.Smmol), potassium bromide (2.3g, 19.2mmol) and concentrated sulphuric acid (0.66ml) in water (7ml). The mixture was stirred for Shours at 0°C then at ambient temperature for 16hours. The product was extracted into diethyl ether (4 x 20ml) then the combined ethereal layers were washed with saturated aqueous sodium chloride solution (2 x 50ml), dried (MgSCU) and the solvent removed in vacua. The residue was purified by chromatography over silica gel eluting with a gradient of methanol: dichloromethane 1:50 -^ 1:20. Appropriate fractions were combined and the solvents removed in vacua to give (#)-a-bromocyclohexanepropionic acid as a pale yellow oil 0.374 g, (27%). TLC (single spot, Rf = 0.45, 10% methanol in dichloromethane), HPLC-MS (single main peak with Rt = 8.88mins, 234.1 / 236.1 [M + Hf, 257.2 / 259.2 [M + Naf). 5H (400MHz, CDC13 at 298K), 0.83-1.88 (11H, CH(cyclohexane), CH2 (cyclohexane), m), 1.89-2.05 (2H(3, m), 4.32-4.44 (IHa, m). (b)Preparation of 3-cyclohexyl-25r-(furan-2-ylmethylsulfanyl)propionic acid (Compound (41), Scheme 9) (#)-a-bromocyclohexanepropionic (0.37g, 1.58rnmol) and furan-2-yl methanethiol (0.18g, 1.58mmol) were dissolved in dimethylformamide (10ml) and purged with nitrogen for lOminutes. The solution was cooled to 0°C then triethylamine (0.22ml, 1.58mmol) was added drop-wise over 1 minute. The mixture was stirred at 0°C for SOminutes then at ambient temperature for 16hours. The solvent was removed in vacuo, and the residue purified by chromatography over gel silica using methanol : dichloromethane 1:100 as eluent. Appropriate fractions were combined and the solvents removed in vacuo to give 3-cyclohexyl-2iS-(furan-2-ylmethyl sulfanyl)propionic acid (41) as a light brown oil, yield 142mg, (33%). Analytical HPLC peak Rt = 18.68mins. TLC (single spot, Rf = 0.45, 10% methanol in dichloromethane), HPLC-MS (single main peak with Rt = 9.53mins, 291.0 [M + Naf). 6H (400MHz, CDC13 at 298K), 0.68-0.89 (2H, CH2(cyclohexane), m), 0.97-1.77 (11H, CH(cyclohexane), CH2(cyclohexane), m), 3.21-3.32 (IHgt, t, J= 7.79 Hz), 3.69-3.79 (1H, CH2S, d, J = 14.79 Hz), 3.84-3.94 (1H, CHaS, d, J= 14.8 Hz), 6.15-6.28 (2H, furan H-3 and H-4, d, J= 27.34 Hz), 7.30 (1H, furan H-5, s). Compound (41) was coupled under standard conditions to loaded building block-linker construct (22). The intermediate loaded thioether (1.2umole gear) was oxidised with m-chloroperbenzoic acid (5eq, 65% reagent, 1.6mg) in dichloromethane (200uL) for 5hrs, followed by standard washing and then cleaved to provide EXAMPLE 76. EXAMPLE 77. (3aS, 6aK) 2^-Cyclohexylmethyl-4-morpholin-4-yl-l-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)butane-l,4-dione HPLC-MS 393.1 [M + H]+, 807.3 [2M + Naf. (a) Preparation of 2/?-cyclohexylmethyl-4-morpholin-4-yl-4-oxo-butyric acid methyl ester. l-(3-dimethylarninopropyl)-3-ethylcarbodiirnide.HCl (1.12g, 5.69mmol) then 1-hydroxybenzotriazole monohydrate (0.87g, 5.69mmol) were added to a stirred solution of 2^?-(cyclohexylmethyl)succinic acid 1 -methyl ester (compound (35),1.0g, 4.38mmol) in dimethylformamide (10ml) at 0°C under argon. The mixture was stirred for 25minutes then morpholine (0.7 ml, 8.76mmol) was added drop-wise over 1 minute and stirring continued at ambient temperature for 16hours. The product was extracted into ethyl acetate (200ml) then washed with l.OM hydrochloric acid (3 x 100ml), saturated aqueous sodium hydrogen carbonate solution (3 x 100ml), water (100ml), then saturated aqueous sodium chloride solution (100ml), dried (MgSCU), and the solvent removed in vacua to give 2/?-cyclohexylmernyl-4-morpholin-4-yl-4-oxo-butyric acid methyl ester as an off-white solid, yield 1.22g, (94%). HPLC-MS (single peak with Rt = 7.91mins, 298.1 [M + H]+, 617.3 [2M + Naf). (b)Preparation of 2/2-cyclohexylmethyl-4-morpholin-4-yl-4-oxo-butyric acid (Compound (36), Scheme 7). A solution of lithium hydroxide monohydrate (O.Slg, 12.18mmol) in water (27ml) was added a stirred solution of 2JR-cyclohexyhnethyl-4-morpholin-4-yl-4-oxo-butyric acid methyl ester (1.21g,, 4.06mmol) in tetrahydrofuran (55ml) and methanol (27ml) at 0°C. The mixture was stirred at ambient temperature for 1 hours then diluted with water (100ml). The aqueous layer was extracted with diethyl ether (2 x 50ml) which was discarded, then acidified to pH = 1-2 with 1M hydrochloric acid. The product was extracted into dichloromethane (3 x 50ml), then the combined ethereal layers washed with water (2 x 50ml), saturated aqueous sodium chloride solution (2 x 50ml), dried (MgSO4) and the solvent removed in vacua to leave a residue. The residue was purified by chromatography over silica gel eluting with a gradient of methanol : dichloromethane 1:100 -> 3:100. Appropriate fractions were combined and the solvents removed in vacuo was to give 2#-cyclohexylmemyl-4-morpholin-4-yl-4-oxo-butyric acid (36) as a white solid, yield 0.82 g, (71%). HPLC-MS (single peak with Rt = 6.769mins, 284.2 [M + Hf, 589.2 [2M + Naf). 5H (400MHz, CDC13 at 298K),0.77-0.90 (2H, CH2(cyclo} ie), m), 1.05-1.40 (4H, CH2(cyclohexane),m), 1.50-1.90 (7H, CH(cyclohexaik CH2(cyclohexane), m), 2.30-2.44 (2HJ3, m), 2.64-2.77 (IHa, m), 2.96-3.10 (1H, OH, brs), 3.40-3.78 (8H, CH2OCH2 and CH2NCH2 ,m). Compound (36) was coupled under standard conditions to loaded building block-linker construct (22), then cleaved to provide EXAMPLE 77. EXAMPLE 78. (3aS, 6aR) 4-(2-Biphenyl-3-yl-4- methylpentanoyl)tetrahydrofuro[3,2-6]pyrrol-3 -one HPLC Rt = 19.09-20.76mins (>90%), HPLC-MS 378.4 [M + H]+ (a)Preparation of Biphenyl-3-yl-acetic acid methyl ester (Compound (38), Scheme 8) Concentrated sulphuric acid (588uL) was added to a solution of 3-bromophenyl acetic acid (lOg, 46.5mmol) in methanol (lOOmL). The mixture was refiuxed for 1.5h and then cooled to ambient temperature and evaporated under reduced pressure to afford a residue. The residue was redissolved in diethyl ether (500mL), washed with water (2 x lOOmL), brine (lOOmL), dried (MgSO4) and then evaporated under reduced pressure to afford 3-bromophenyl acetic acid methyl ester (10.65g). The 3-bromophenyl acetic acid methyl ester was dissolved in toluene (117mL) then phenyl boronic acid (6.8g, 55.69mmol) added, followed by a aqueous solution of sodium carbonate (93mL, 2M) and tetrakis(triphenylphosphine)palladium (1.6g, 1.41mmol). The mixture was stirred overnight then cooled to ambient temperature and an aqueous solution of saturated ammonium chloride (lOOmL) added. The mixture was extracted with ethyl acetate (2 x 200mL), died (Na2SO4) and evaporated under reduced pressure to afford a residue. Flash chromatography of the residue over silica (200g) using ethyl acetate : heptane (3 : 48) as the eluent gave biphenyl-3-yl acetic acid methyl ester, yield 10.5g, (99 %), TLC (single UV spot, R{ = 0.24, 10% ethyl acetate in heptane), analytical HPLC Rt - 19.55 min, HPLC-MS (single main UV peak with Rt = 9.35 min, 227.1 [M + H]). 5H (400MHz, CDC13 at 298K) 3.76 (2H, s, CH2CO2CH3), 3.77 (3H, s, OCH3\ 7.34-7.66 (9H, m, biphenyl-3-yl). (b)Preparation of Biphenyl-3-yl-acetic acid Water (39mL), followed by lithium hydroxide monohydrate (4.2g, lOl.Smmol) were added to a solution of biphenyl-3-yl acetic acid methyl ester (11.43g, 50.57mmol) in methanol (265mL). The mixture was stirred at ambient temperature for 2h then the organics were removed under reduced pressure. The mixture was acidified with dilute hydrochloric acid (1M, 80mL), extracted with chloroform (2 x lOOmL), dried (MgS04) and evaporated under reduced pressure to afford biphenyl-3-yl acetic acid as a white solid, yield 10.6g, (99%), analytical HPLC /?t = 16.565 min, HPLC-MS (single main UV peak with RI = 7.91mm, 213. 1 [M + Hf). 8H (400MHz, CDC13 at 298K) 3.77 (2H, s, C^CC^CHj), 7.28-7.52 (9H, m, biphenyl-3-yl). (c)Preparation of 2/?S-Biphenyl-3-yl-4-rnethylpent-4-enoic acid A solution of biphenyl-3-yl acetic acid (7.0g, 33mmol) in anhydrous tetrahydrofuran (84mL) was added dropwise to a solution of lithium diisopropyl amide (36.4mL, 2M solution in hexanes) in anhydrous tetrahydrofuran (84mL) at -78 °C. The mixture was allowed to warm to 0 °C and stirred for 40 min. The mixture was then cooled to -78 °C and 3-bromo-2-methylpropene (4.97mL) rapidly added. The mixture was stirred for 1 h at -78 °C then water (28mL) added and the organics removed under reduced pressure. The mixture was then acidified with hydrochloric acid (6M, 14ml), extracted with ethyl acetate (3 x 100 ml), dried (MgS04) and evaporated under reduced pressure to afford a residue. Flash chromatography of the residue over silica (400g) using methanol : dichloromethane (3 : 97) as the eluent afforded impure 2-biphenyl-3-yl-4-methylpent-4-enoic acid (8.3 g). Flash chromatography over silica (400g) using methanol: dichloromethane (1.5 : 98.5) afforded pure 2-biphenyl-3-yl-4-methylpent-4-enoic acid, yield 5.27g, (60 %), TLC (single UV spot, Rf = 0.28, 5 % methanol hi dichloromethane), analytical HPLC J?t = 19.99 min, HPLC-MS (single main UV peak with ft = 9.57 min, 267.1 [M + H]4). SH (400MHz, CDC13 at 298K), 1.765 (3H, s, C#3)> 2.53 (1H, dd, J= 6.6 and 14.7 Hz, 3-Hi), 2.91 (1H, dd, J= 8.9 and 14.7 Hz, 3-Hi), 3.92 (1H, dd, /= 6.6 and 8.9 Hz, 2-H), 4.79 (2H, d, J= 10.7 Hz, 5-H2), 7.30-7.62 (9H, m, biphenyl-3-yl). (d)Preparation of 2&S-Biphenyl-3-yl-4-methylpentanoic acid (Compound (39), Scheme 8) Palladium on carbon (10%, 300mg) was added portionwise to a solution of 2RS-biphenyl-3-yl-4-methylpent-4-enoic acid (Ig, 3.76mmol) in ethanol (40mL) at 0 °C. A hydrogen atmosphere was then introduced and the mixture allowed to warm to ambient temperature. The mixture was stirred for 18h, then the hydrogen atmosphere removed and the mixture filtered over Celite and the catalyst washed with ethanol (40mL). The combined organic filtrate was concentrated under reduced pressure to afford a residue, which was flash chromatographed over silica (150g) using methanol : dichloromethane (1 : 99) as the eluent to afford 2^1S-biphenyl-3-yl-4-methylpentanoic acid, yield 980mg, (98%), TLC (single UV spot, R{ = 0.45, 5 % methanol in dichloromethane), analytical HPLC Rt = 20.92 min, HPLC-MS (single main UV peak with Rt = 10.15 min, 269.1 [M + H]+, 291.1 [M+Na]4). 5H (400MHz, CDC13 at 298K), 0.93 (6H, d, J= 6.6 Hz, 2 x CH3), 1.52-1.57 (IH, m, 4-HO, 1.71-1.76 (IH, m, 3-Hi), 1.97-2.05 (IH, m, 3-Hi), 3.66 (IH, t, J= 7.8 Hz, 2-Hi), 7.32-7.60 (9H, m, biphenyl-3-yl). Compound (39) was coupled under standard conditions to loaded building block-linker construct (22), then cleaved to provide EXAMPLE 78. EXAMPLE 79. (3aS, 6aK) 4-Methyl-JV-[3-methyl-15-(3-oxo-hexahydrofuro[3,2-&] pyrrole-4-carbonyl) butyl]benzamide HPLC Rt = 13.74-14.85mins (> 90%), HPLC-MS 359.2 [M + Hf. HPLC Rt = 12.95-13.87mins (> 90%), HPLC-MS 375.2 [M + Hf. EXAMPLE 80. (3aS, 6aR) 4-Methoxy-^-[3-methyl-15-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]benzamide (Figure Remove)-174- ^-Butyl-N-[lS-(4-bromobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]benzamide (Figure Remove)HPLC Rt = 18.40-19. lOmins (> 90%), HPLC-MS 513.2 / 515.2 [M + Hf. EXAMPLE 82. (3aS, 6aR) 4-fert-Butyl-]V-[15-(4-methoxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fc]pyrrol-4-yl)ethyl]benzamide HPLC Rt = 17.50-18.31mins (> 90%), HPLC-MS 465.2 [M + H]+. EXAMPLE 83 (An example of general formula (II)). (3aS, laK) Morpholine-4-carboxylic acid [l/?-ben2ylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3J2-^]pyridin-4-yl)ethyl] amide The required bicycle building block (3a5, 7aR) 3-oxo-hexahydrofuro[3,2-6]pyridine-4-carboxylic acid 9H-fluoren-9-ylmethyl ester was prepared in 8 steps as follows. (1) Preparation of Cz5-7-ac-(3-hydroxy)pyrrolidine-l,2-dicarboxylic acid 1-(9H-fluoren-9-ylmethyl) ester. O'5r-rac-(3-hydroxy)piperidine-2-carboxylic acid (obtained by hydrogenation of commercially available 3-hydroxypicolinic acid, see Drummond, J. et al, J. Med. Chem., 32, 2116-2128, 1989) (15.0g, 103.4mmole) was added to a vigorously stirred, ice-cooled solution of sodium carbonate (22.90gs 217.1mmole) in water (200mL). 1,4-Dioxan (lOOmL) was added providing a clear pale yellow solution. 9-Fluorenylmethyl chloroformate (27.52g, 108.4mmole) in 1,4-dioxan (lOOrnL) was added over Ihr, then the ice-cooling removed and the mixture stirred at RT for an additional Ihr. Additional water (150mL) was added, the reaction mixture washed with chloroform (2 x 250mL) and the combined organic layers discarded. The aqueous phase was acidified with IN HC1 to ~ pH 2, providing a thick opaque mixture. The acidified aqueous mixture was extracted with chloroform (2 x SOOmL) and the now clear aqueous phase discarded. The opaque combined chloroform layers were dried (NaiSO-O, filtered and reduced in vacuo to provide a white foam, yield 30.2g (82.2mmole, 79.5%). TLC (Minor UV spot, Rf = 0.58, major UV spot Rf = 0.25, methanol: chloroform 1:4), analytical HPLC major peak Rt = 16.990mins, HPLC-MS (single major UV peak with Rt = 7.976mins, 368.0 [M + H]+, 390.0 [M + Naf. Analysis by LH and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond. 5C (DMSO-d6 at 298K); 23.29/23.57 (d, C5), 28.89 (d, Cy), 40.19 (d, Ce), 47.59 (u, Fmoc C-9), 59.11/59.43 (u, Ca), 67.34 (u, Cp), 67.75/67.87 (d, Fmoc CH2), 121.13 (u, Fmoc C-4 and C-5), 126.90/127.02 (u, Fmoc C-l and C-8), 128.11 (u, Fmoc C-2 and C-7), 128.68 (u, Fmoc C-3 and C-6), 141.73 (q, Fmoc C-4' and C-5'), 144.58/144.76 (q, Fmoc C-l' and C-8'), 155.71/156.23 (q, OCON), 172.37/172.63 (COOH). (2) Preparation of Cw-rac-(3-hydroxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester l-(9H-fluoren-9-ylmethyl) ester. Cw-7-ac-(3-hydroxy)pyrrolidine-1,2-dicarboxylic acid 1 -(9H-fluoren-9-yl methyl) ester (28.2g, 76.7mmole) was dissolved in toluene (200mL) in a Dean-Stark apparatus. Allyl alcohol (50mL) was added followed by p-toluenesulphonic acid.hydrate (15.Ig, 78.2mmole). The mixture was refluxed for Ihr, cooled and CHCls (SOOmL) added. The organic layer was washed with NaHCO3 (2 x 250mL), 0.1N HC1 (250mL) and brine (250mL), then dried (Na2SO4). Filtration and reduction in vacuo gave a pale yellow mobile oil (35g). The crude oil was purified over silica gel (240g) eluting with a gradient of heptane : ethyl acetate 3:1 -^ 1:1. Desired fractions were combined and reduced in vacuo to a colourless gum yield 20.4g (SO.lmmole, 65.3%). TLC (single UV spot, Rf = 0.20, heptane : ethyl acetate 2:1), analytical HPLC Rt = 20.003mins, HPLC-MS (single main UV peak with Rt = 9.473mins, 408.1 [M + Hf, 430.1 [M + Naf). Analysis by !H and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond. 5H (CDC13 at 298K); 1.55 (2Hyor 2H5, d, J9.3), 1.81 (lHy or 1H5, d, J3.2), 2.06 (lHYor 1H5, d, J3.2), 2.78/2.92 (1HE, dbt), 3.55 (1HP, dd, J12.6, 9.2), 3.78 (OH, b), 3.95/4.11 (1H£, d, J13), 4.31 (Fmoc H-9, m), 4.49 (Fmoc CHj, m), 4.71 (2 x COOCH2CH=CH2, m), 4.99/5.17 (Ha, d, J4.8), 5.31 (2 x COOCH2CH=CH2j m), 5.87 (1 x COOCH2CH=CH2, m), 7.37 (2H aromatic, Fmoc H-2 and H-7), 7.47 (2H aromatic, Fmoc H-3 and H-6), 7.62 (2H aromatic, Fmoc H-l and H-8), 7.83 (2H aromatic, Fmoc H-4 and H-5). 6C (CDC13 at 298K); 23.87/24.11 (d, C5), 30.59 (d, Cy), 41.26/41.56 (d, Ce), 47.62 (u, Fmoc C-9), 58.29/59.54 (u, Ctt), 66.44/66.54 (d, COOCH2CH=CH2), 68.28 (d, Fmoc CH2), 69.14/69.42 (u, Cp), 119.67/119.90 (d, COOCH2CH=CH2), 120.43 (u, Fmoc C-4 and C-5), 125.22/125.35 (u, Fmoc C-l and C-8), 127.48 (u, Fmoc C-2 and C-7), 128.15 (u, Fmoc C-3 and C-6), 131.43/131.57 (u, COOCH2CH=CH2), 141.73 (q, Fmoc C-4' and C-5'), 144.19/144.38 (q, Fmoc C-l' and C-8'), 155.67/156.27 (q, OCON), 171.56 (COOCH2CH=CH2). (3) Enzymic Resolution of Cis-rac-(3-hydroxy)pyrroUdine-l,2-dicarboxylic acid 2-allyl ester l-(9H-fluoren-9-ylmethyl) ester to (2R, 35) (3-hydroxy) pyrrolidine-l,2-dicafboxylic acid 2-allyl ester l-(9H-fluoren-9-ylmethyl) ester and (25, 2>R) (3-acetoxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester l-(9H-fluoren-9-ylmethyl) ester. Cw-rac-(3-hydroxy)pyrrolidine-l,2-dicafboxylic acid 2-allyl ester 1-(9H-fluoren-9-ylmethyl) ester (19.6g, 48.1mmole), Lipase PS (25g, ex Amano Enzyme Inc.), vinyl acetate (300mL) and isopropyl ether (200mL) were stirred at 38°C. After 5 days, HPLC indicated equilibration of 2 peaks (52% : 48%, indicating 95% conversion) and the reaction mixture was filtered through celite (3cm x 10cm bed). The celite bed was washed with chloroform (3 x 250mL) and the combined organic filtrate reduced in vacuo to a viscous tan oil (23.Og). The crude oil was purified over silica gel (300g) eluting with a gradient of heptane : ethyl acetate 3:1 -^ 1:2. Two major fractions were identified and reduced in vacuo. Product 1. (25, 2>K) (3-acetoxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester l-(9H-fluoren-9-yknethyl) ester. A viscous very pale yellow oil, yield 9.5g (21.1mmole, 43.9%). TLC (single UV spot, Rf = 0.40, heptane : ethyl acetate 2:1), analytical HPLC Rt = 22.633mins, HPLC-MS (single main UV peak with Rt = 10.714mins, 450.1 [M + Hf, 472.1 [M + Na]4). Analysis by JH and ]3C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond. 5H (CDC13 at 298K); 1.25-1.32/1.52-1.70/1.72-2.00 (2HY + 2H5, bm), 2.05- 2.15 (OCOCHj, bs), 3.35-3.58 (1HE, m), 3.98-4.10/4.18-4.22 (1HE, dd), 4.24-4.31 (Fmoc H-9, m), 4.32-4.52 (Fmoc CKg, m), 4.60-4.82 (2 x COOCH2CH=CH2, m), 4.92-4.99 (Hp, m), 5.22-5.32 (Ha, m), 5.28- 5.32/5.34-5.41 (2 x COOCH2CH=CH2, dd, .717.2), 5.89-6.00 (1 x COOCH2CH=CH2, m), 7.30-7.37 (2H aromatic, Fmoc H-2 and H-7), 7.38- 7.46 (2H aromatic, Fmoc H-3 and H-6), 7.59-7.76 (2H aromatic, Fmoc H-1 and H-8), 7.78-7.80 (2H aromatic, Fmoc H-4 and H-5, d, J7.5). 5C (CDC13 at 298K); 21.22 (u, OCOCH3), 23.01 (d, C5), 25.54 (d, Cy), 40.51/40.88 (d, CE), 47.44 (u, Fmoc C-9), 55.55 (u, Ca), 66.08 (d, COOCH2CH=CH2), 68.30/68.65 (d, Fmoc CH2), 69.21/69.59 (u, Cp), 118.94/119.22 (d, COOCH2CH=CH2), 120.31 (u, Fmoc C-4 and C-5), 125.34/125.49 (u, Fmoc C-1 and C-8), 127.43 (u, Fmoc C-2 and C-7), 128.04 (u, Fmoc C-3 and C-6), 131.93 (u, COOCH2CH=CH2), 141.61 (q, Fmoc C-4' and C-5'), 144.15 (q, Fmoc C-1' and C-8'), 155.50/156.36 (q, OCON), 169.42/169.91/170.37 (COOCH2CH=CH2 + OCOCH3). Product 2. (2R, 35) (3-hydroxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester l-(9H-fluoren-9-ylmethyl) ester. A white crystalline solid, yield 9.05g (22.2mmole, 46.1%). TLC (single UV spot, Rf = 0.20, heptane : ethyl acetate 2:1), analytical HPLC Rt = 19.970mins, HPLC-MS (single main UV peak with Rt = 9.450mins, 408.1 [M + H]+, 430.1 [M + Na]4). Analysis by H and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond. 5H (CDC13 at 298K); 1.47-1.61 (lHy+ 1H5, m), 1.71-1.81 (1H5, b), 2.02-2.11 (1HY> b), 2.71-2.81/2.84-2.94 (1HE, dt), 3.54-3.59 (Hp, m), 3.70-3.82 (OH, b), 3.93-3.96/4.10-4.16/4.11 (1H6, d, JIO), 4.24-4.31 (Fmoc H-9, m), 4.40-4.56 (Fmoc CH^, m), 4.61-4.78 (2 x COOCH2CH=CH2} m), 4.98/5.18 (Ha, d, J5.0), 5.25-5.37 (2 x COOCH2CH=CH2, m), 5.86-5.96 (1 x COOCH2CH=CH2, m), 7.30-7.34 (2H aromatic, Fmoc H-2 and H-7), 7.41-7.48 (2H aromatic, Fmoc H-3 and H-6), 7.53-7.62 (2H aromatic, Fmoc H-l and H-8), 7.79-7.81 (2H aromatic, Fmoc H-4 and H-5). 8C (CDC13 at 298K); 23.87/24.12 (d, C6), 30.60 (d, C,\ 41.27/41.57 (d, Ce), 47.62 (u, Fmoc C-9), 58.29/59.54 (u, Ca), 66.44/66.54 (d, COOCH2CH=CH2), 68.28 (d, Fmoc CH2), 69.15/69.42 (u, Cp), 119.67/119.91 (d, COOCH2CH=CH2), 120.42 (u, Fmoc C-4 and C-5), 125.22/125.35 (u, Fmoc C-1 and C-8), 127.48 (u, Fmoc C-2 and C-7), 128.15 (u, Fmoc C-3 and C-6), 131.42/131.57 (u, COOCH2CH=CH2), 141.73 (q, Fmoc C-4' and C-5'), 144.12/144.37 (q, Fmoc C-1' and C-8'), 155.67/156.26 (q, OCON), 171.57 (COOCH2CH=CH2). (4) Preparation of (25, 3£) (3-hydoxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester l-(9H-fluoren-9-ylmethyl) ester. (25, 3K) (3-acetoxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester 1-(9H-fluoren-9-ylmethyl) ester (4.5g, lOmmole) ) was dissolved in allyl alcohol (50mL), cH2SO4 (200uL) added and heated to reflux for 24hrs. The mixture was cooled, EtOAc (250mL) added and washed with NaHCOj (250mL), brine (250mL) and dried (Na2SC«4). The solvents were removed in vacuo to give a tan oil (4.2g). The crude oil was purified over silica gel (170g) eluting with a gradient of heptane : ethyl acetate 3:1 -> 1:1. Desired tractions were combined and reduced in vacuo to a white crystalline solid, yield 3.0g (7.37mmole, 73.7%). TLC (single UV spot, Rf = 0.20, heptane : ethyl acetate 2:1), analytical HPLC Rt = 19.474mins, HPLC-MS (single main UV peak with Rt = 9.507mins, 408.2 [M + Hf, 430.2 [M + Naf). Analysis by 1H and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond. 5H (CDC13 at 298K); 1.46-1.59 (1HY+ 1H8, m), 1.74-1.81 (1H8, b), 2.01-2.10 (lHy, b), 2.70-2.82/2.88-2.99 (lHe, dt), 3.49-3.62 (Hp, m), 3.70-3.80 (OH, b), 3.90-3.94/4.09-4.14 (1H6, dd), 4.26-4.32 (Fmoc H-9, m), 4.41-4.58 (Fmoc CH^, m), 4.60-4.80 (2 x COOCH2CH=CH2, m), 4.96-4.98/5.17-5.18 (Ha, dd, J4.8), 5.25-5.42 (2 x COOCH2CH=CH2, m), 5.82-5.97 (1 x COOCH2CH=CH2, m), 7.30-7.38 (2H aromatic, Fmoc H-2 and H-7), 7.42-7.48 (2H aromatic, Fmoc H-3 and H-6), 7.55-7.63 (2H aromatic, Fmoc H-l and H-8), 7.79-7.84 (2H aromatic, Fmoc H-4 and H-5). 5C (CDC13 at 298K); 23.86/24.12 (d, C8), 30.62 (d, Cy), 41.28/41.57 (d, CE), 47.62 (u, Fmoc C-9), 58.29/59.54 (u, Ca), 66.43/66.54 (d, COOCH2CH=CH2), 68.27 (d, Fmoc CH2), 69.15/69.43 (u, Cp), 119.69/119.91 (d, COOCH2CH=CH2), 120.42 (u, Fmoc C-4 and C-5), 125.22/125.35 (u, Fmoc C-l and C-8), 127.47 (u, Fmoc C-2 and C-7), 128.14 (u, Fmoc C-3 and C-6), 131.43/131.57 (u, COOCH2CH=CH2), 141.73 (q, Fmoc C-4' and C-5'), 144.12/144.37 (q, Fmoc C-l' and C-8'), 155.67/156.26 (q, OCON), 171.57 (COOCH2CH=CH2). (5) Preparation of (2S, 3K) (3-tert-butoxy)pyrrolidine-l ,2-dicarboxylic acid 2-allyl ester l-(9H-fluoren-9-ylmethyl) ester. (25, 3K) (3-hydoxy)pyrrolidine-l ,2-dicarboxylic acid 2-allyl ester 1-(9H-fluoren-9-ylmethyl) ester (1.75g, 4.30mmole) was dissolved in dry dichloromethane (20mL) in a SOmL glass pressure tube and cooled to -78°C. Isobutylene gas (~10mL) was condensed into the solution and cH2SC«4 (lOOuL) added. A stirrer bar was added, the tube was sealed, the cooling removed and stirred at RT for 72hr. The sealed tube was cooled to -78°C, N-methylmorpholine (200uL, leq w.r.t. cH2SO4) and allowed to warm to RT, unsealed, with stirring over 2hr. Dichloromethane (75mL) was added and the organics washed with NaHCO3 (75mL), then brine (75mL) and dried (Na2SO4). The solvents were removed in vacuo to give a pale tan oil (1.91g). The crude oil was purified over silica gel (lOOg) eluting with a gradient of heptane : ethyl acetate 5:1 -^ 3:1. Desired fractions were combined and reduced in vacuo to a thick clear oil yield 1.59g (3.43mmole, 79.8%). TLC (single UV spot, Rf= 0.50, heptane : ethyl acetate 2:1), analytical HPLC Rt = 24.123mins, HPLC-MS (single main UV peak with Rt = 11.91mins, 408.2 [M + H-Bu'f, 486.3 [M + Naf, 949.5 [2M + Na]4). Analysis by !H and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond. sH (CDC13 at 298K); 1.23 (9 x C(CH3)3, s), 1.46-1.98 (2HY+ 2H5, m), 3.37-3.45/3.46-3.57 (1HE, dt), 3.61-3.72 (Hp, b), 3.90-3.99/4.02-4.10 (lHe, dd), 4.20-4.72 (Fmoc H-9 + Fmoc CH^ + 2 x COOCH2CH=CH2, bm), 4.89/5.01 (Ha, dd, J6.3), 5.24 (1 x COOCH2CH=CH2, d, J10.5), 5.38 (1 x COOCH2CH=CH2, d), 5.88-5.99 (1 x COOCH2CH=CH2, m), 7.29-7.36 (2H aromatic, Fmoc H-2 and H-7), 7.38-7.47 (2H aromatic, Fmoc H-3 and H-6), 7.56-7.69 (2H aromatic, Fmoc H-1 and H-8), 7.76-7.81 (2H aromatic, Fmoc H-4 and H-5). 5C (CDC13 at 298K); 23.69/23.99 (d, C8), 28.38/28.65 (u, C(CH3)3), 28.75 (d, q), 40.40/40.72 (d, Ce), 47.61 (u, Fmoc C-9), 58.36/58.47 (u, Ca), 65.67 (d, COOCH2CH=CH2), 67.82 (u, Cp), 67.99/68.13 (d, Fmoc CH2), 74.97 (q, C(CH3)3), 118.44/118.57 (d, COOCH2CH=CH2), 120.40 (u, Fmoc C-4 and C-5), 125.39/125.48 (u, Fmoc C-l and C-8), 127.46 (u, Fmoc C-2 and C-7), 128.10 (u, Fmoc C-3 and C-6), 132.49 (u, COOCH2CH=CH2), 141.71 (q, Fmoc C-4' and C-5'), 144.14/144.30 (q, Fmoc C-l' and C-8'), 155.44/157.00 (q, OCON), 170.43/171.05 (COOCH2CH=CH2). (6) Preparation of (2S, 3K) (3-tert-butoxy)pyrrolidine-l,2-dicarboxylic acid 1-(9H-fluoren-9-ylmethyl) ester. (25,3^) (3-fe7-/-butoxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester 1-(9H-fluoren-9-ylmethyl) ester (1.52g, 3.29mmole) was dissolved in dry dichloromethane (25mL) with stirring. Tetrakistriphenylphospuine palladium (0) (76mg, 0.066mmole, 0.02eq) was added, followed by phenyltrihydrosilane (0.71 g, 0.622mL, 6.58mmole, 2eq). After Ihr, dichloromethane (150mL) was added and the organics washed with 0.01N HC1 (ISOmL), brine (150mL) and dried (Na2SO4). The solvents were removed in vacuo to give a dark grey solid (2.05g). The crude solid was purified over silica gel (75g) eluting with a gradient of heptane : ethyl acetate 2:1 -> 1:2. Desired fractions were combined and reduced in vacuo to a white crystalline yield 0.97g (2.29mmole, 69.6%). TLC (single UV spot, Rf = 0.25, heptane : ethyl acetate 1:1), analytical HPLC Rt = 21.310mins, HPLC-MS (single main UV peak with Rt = 10.254mins, 368.2 [M + H-Bulf, 446.2 [M + Na]+, 869.3 [2M + Na]4). Analysis by 1E and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond. 5H (CDC13 at 298K); 1.32/1.40 (9 x C(CH3)3, ds), 1.42-1.58 (1HY+ 1H5, m), 1.72-1.80 (1H5, bm)3 1.82-1.96 (1HY, bm), 2.76-2.82/2.90-2.97 (1H£5 dt), 3.70-3.84 (Hp, b), 3.90-4.07 (lHe, dd), 4.22-4.32 (Fmoc H-9), 4.40-4.58 (Fmoc CH2, bm), 4.59-4.61/5.02-5.04 (Ha, dd, J5.7), 7.29-7.36 (2H aromatic, Fmoc H-2 and H-7), 7.39-7.43 (2H aromatic, Fmoc H-3 and H-6), 7.53-7.67 (2H aromatic, Fmoc H-l and H-8), 7.76-7.81 (2H aromatic, Fmoc H-4 and H-5), 10.90-11.10 (COOH,bs). 6C (CDC13 at 298K); 23.71/24.23 (d, C5), 28.21/28.28 (u, C(CH3)3), 30.58/30.72 (d, Cy), 40.49/40.79 (d, Ce), 47.58/47.63 (u, Fmoc C-9), 58.20/58.43 (u, Ca), 67.78/68.32 (u, Cp), 68.69/68.88 (d, Fmoc CH2), 78.48/78.67 (q, C(CH3)3), 120.31 (u, Fmoc C-4 and C-5), 125.16/125.27/125.50/125.53 (u, Fmoc C-l and C-8), 127.42/127.46/127.59 (u, Fmoc C-2 and C-7), 128.03/128.10 (u, Fmoc C-3 and C-6)5 141.55/141.69/141.79 (q, Fmoc C-4' and C-5'), 144.06/144.12/144.38/144.48 (q, Fmoc C-l' and C-8')5155.73/156.49 (q, OCON), 169.26/169.52 (COOH). (7) Preparation of (2S, 3R) (3-ter^butoxy)-2-(2-diazoacetyl)pyn:oHdine-l32-dicarboxylic acid l-(9H-fluoren-9-ylmethyl) ester. (25, 37?) (3-ter/-butoxy)pyrrolidme-l,2-dicarboxylic acid 1-(9H-fluoren-9-yhnethyl) ester (830mg, 1.96mmole) was dissolved with stirring in dry dichloromethane (20mL). The reaction was flushed with nitrogen and cooled to -15°C. Isobutylchloroformate (296mg, 2.16mmole in dry dichloromethane (2.5mL) and N-methyhnorpholine (397mg, 3.92mmole in dry dichloromethane (2.5mL) were added simultaneously in O.SmL aliquots over ISmrns. Etheral diazomethane (generated from diazald (2.5g, ~8mmole in diethyl ether (40mL)) onto sodium hydroxide (2.75g) in water (4.3mL) / ethanol (8.6mL) at 60°C). was added to the activated aminoacid solution and stirred at RT for 24hr. Acetic acid (~2mL) was added to quench the reaction, then fe/t-butyhnethylether (lOOmL) was added, the organics washed with water (3 x 150mL), then dried (Na2SO4). The solvents were removed in vacuo to give a thick yellow oil (1.02g). The crude oil was used for the next stage without purification. TLC (single main UV spot, Rf = 0.40, heptane : ethyl acetate 2:1), analytical HPLC Rt = 18.887mins (50.8%) plus numerous minor peaks, HPLC-MS (2 main UV peaks with Rt = 9.095mins, 364.2 [M + Hf, 749.2 [2M + Naf and Rt = 10.856mins, 420.2 [M + H-N2]+, 470.2 [M + Naf, 917.3 [2M + Naf). Note that upon HPLC-MS analysis, m/z 364.2 corresponds to the desired bicycle product (3aS, 7a/?) 3-oxo-hexahydrofuro[3,2-Z)]pyridme-4-carboxylic acid 9#-fluoren-9-ylmethyl ester. (8) Cyclisation to (3aS, laK) 3-oxo-hexahydrofuro[3,2-6]pyridine-4-carboxy lie acid 9/f-fluoren-9-ylmethyl ester. A solution of lithium chloride (844mg, 20mmole) in water (5mL) and acetic acid (20mL) was added to (25, 3R) (3-/ert-butoxy)-2-(2-diazoacetyfypyrrolidine-l^-dicarboxylic acid l-(9H-fluoren-9-ylmethyl) ester (lOOOmg, ~2mmole). Gas was evolved and the yellow oily solid dissolved over Ihr to give a virtually colourless solution. After 90mins, chloroform (150mL) was added and the organics washed with NaHCO3 (2 x ISQmL), brine (150mL) and dried (NaaSO/j). The solvents were removed in vacuo to give a pale yellow gum (920mg). The crude gum was purified over silica gel (135g) eluting with a gradient of heptane : ethyl acetate 3:1 -> 1:1. Desired fractions were combined and reduced in vacuo to a white crystalline solid, yield 370mg (1.02mmole, 51.9% from starting acid). TLC (single UV spot, Rf = 0.25, heptane : ethyl acetate 2:1), analytical HPLC Rt = 18.734mins, HPLC-MS (single UV peak with Rt = 9.051mins, 364.2 [M + Hf, 386.2 [M + Na]4). Analysis by !H and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond. sH (CDC13 at 298K); 1.29-1.49 (1HY+ 1H5, m), 1.61-1.80 (1H5, bm), 2.06-2.19 (1HY, bm), 2.50-2.62/2.63-2.80 (1H6, m), 3.96 (1HE, b), 3.97 (COCHiA, d, J16.3), 4.15 (Fmoc H-9, bt), 4.25-4.34 (Hp, b), 4.36-4.60 (Fmoc CHi + COCH2B, bm), 4.75-4.82/5.11-5.19 (Ha, bd), 7.30-7.36 (2H aromatic, Fmoc H-2 and H-7), 7.41-7.49 (2H aromatic, Fmoc H-3 and H-6), 7.52-7.68 (2H aromatic, Fmoc H-l and H-8), 7.75-7.85 (2H aromatic, Fmoc H-4 and H-5). SC (CDC13 at 298K); 21.75/22.04 (d, C5), 26.60 (d, Cy), 41.40/41.66 (d, CE), 47.59 (u, Fmoc C-9), 60.42 (u, Ca), 67.54 (d, Fmoc CH2), 68.21/68.39 (d, COCH2O), 72.61 (u, Cp), 120.39 (u, Fmoc C-4 and C-5), 125.43 (u, Fmoc C-1 and C-8), 127.51 (u, Fmoc C-2 and C-7), 128.14 (u, Fmoc C-3 and C-6), 141.73 (q, Fmoc C-41 and C-5'), 144.30 (q, Fmoc C-l' and C-8'), 155.79/156.35 (q, OCON), 211.10/211.4 (q, COCH2O). Following the general details from Scheme 2, the reqmred bicycle building block (3aS, laK) 3-oxo-hexahydrofuro[3,2-fc]pvridine-4-carboxylic acid 9ff-fluoren-9-ylmethyl ester was converted to building block-linker construct as follows: (3aS, 7a/?) 3-oxo-hexahydrofuro[3,2-6]pyridine-4-carboxylic acid 9//-fluoren-9-ylmethyl ester (250mg, 0.689mmole) was dissolved in a mixture of ethanol (12.0mL) and water (1.75mL) containing sodium acetate.trihydrate (141mg, 1.03mmole, 1.5eq). 4-[[(hydrazinocarbonyl)amino]methyl]cyclo hexanecarboxylic acid, trifluoroacetate (227mg, 0.689mmole, l.Oeq, Murphy, A. M. et al, J. Am. Chem. Soc., 114, 3156-3157, 1992) was added and the mixture refluxed for 4brs. Chloroform (150mL) was added and the organics washed with HC1 (150mL, ~ pH3), dried (NaaSC^) and reduced in vacuo to provide crude building block-linker construct a clear viscous oil. Yield 350mg, analytical HPLC 2 peaks Rt = 17.101 (74.7%) and 18.547mins (25.3%) (cis / trans geometrical isomers), HPLC-MS (2 x UV peak with Rt = 8.037 and 8.972mins, 561.2 [M + H]). Crude construct was used directly for construct loading. Following the general details from Scheme 2, the required building block-linker construct was attached to the solid phase providing loaded building block-linker construct as follows: Building block-linker construct (0.595mmoles), 2-(lH-benzotriazole-l-yl)-l, 1,3,3-tetramethyluroniumhexafluoro phosphate (HBTU, 226.0mg, 0.596mmole), 1- rrydroxybenzotriazolehydrate and (HOBT, 92mg, 0.596mmole) were dissolved in dimethylformamide (3mL) and N-methylmorpholine (NMM, 131 \iL, 1.197mmole) added. After pre-activation for 5 minutes, free amine gears (160 x 1.2umole) were added, followed by dimethylformamide (17.5mL) and left overnight. The spent coupling solution was then added to free amine crowns (18 x lOjamole) and left overnight. Standard washing and analyses indicated quantitative loading in both cases. Following the general details from Scheme 2, the required loaded building block-linker construct was elaborated on the solid phase as follows: Loaded construct was elaborated to EXAMPLE 83 (3aS, TaR) Morpholine-4-carboxylic acid [lJl?-benzylsulfanylmethyl-2-oxo-2-(3-oxohexahydrofuro[3,2-fe] pyridin-4-yl)ethyl]amide by standard Fmoc deprotection and sequential coupling with Fmoc-Cys(SBzl)-OH then morpholine-4-carbonylchloride. The crude example was cleaved and analysed (see general techniques). HPLC Rt = 15.59mins (>80%), HPLC-MS 449.2 [M + Hf. The following examples (84 - 130) were prepared as detailed for EXAMPLE 83, coupling with the required reagents to provide the full length molecule. HPLC Rt = 15.79mins (> 80%), HPLC-MS 459.3 [M + Hf. EXAMPLE 84. (3aS, 7a#) Naphthalene-2-carboxylic acid [15-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3s2-6]pyridin-4-yl)ethyl]amide EXAMPLE 85. (3aS, 7aR) 4-Dimethylamino-JV-[15-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-&]pyridin-4-yI)ethyl]benzamide HPLC Rt = 10.98mins (> 80%), HPLC-MS 452.2 [M + H]+. EXAMPLE 86. (3aS, 7a#) Naphthalene-1-cafboxylic acid [3-methyl-15-(3-oxo-hexahydrofuro [3,2-6]pyridine-4-carbonyl) butyl] amide PLC Rt = 17.71mins (> 80%), HPLC-MS 409.2 [M + Hf. EXAMPLE 87. (3aS, 7aR) Naphthalene-2-carboxylic acid [3-methyl-15-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl)butyl]amide(Figure Remove)EXAMPLE 8. (3aS, 7a#) JV-[lS-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydro furo[3,2- HPLC Rt = 13.16mins (> 80%), HPLC-MS 492.2 [M + Hf..ydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-b]pyridin-4-yl)ethyl]amide HPLC Rt = 17.38mins (> 80%), HPLC-MS 485.2 [M + H]+. EXAMPLE 91. (3aS, 7a#) 4-fe^-butyl-Ar-[16'-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-h.exahydrofuro[3,2-6]pyridin-4-yl)ethyl]benzamide HPLC Rt = 17.90mins (> 80%), HPLC-MS 485.2 [M + Hf. EXAMPLE 92. (3aS, 7aR) ^-[15-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexaliydrofuro [3^2-6]pyridin-4-yl)ethyl]-4-thiophen-2-ylbenzaniide HPLC Rt = 17.00mins (> 80%), HPLC-MS 491.2 [M + H]+. EXAMPLE 93. (3aS, 7aR) Ar-[l^-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-liexahydrofuro o,2-Z7]pyridin-4-yl)ethyl]-4-trifluorometlioxybenzamide HPLC Rt = 17.07mins (> 80%), HPLC-MS 493.2 [M + H]+. EXAMPLE 94. (3aS, 7aK) ^-[3-methyl-lS-(3-oxo-hexahydrofuro[3,2-Z)]pyridme-4-carbonyl)butyl]-5-thiophen-2-ylnicotinamide HPLC Rt = 15.82mins (> 80%), HPLC-MS 442.2 [M + H]+. EXAMPLE 95. (3aS, 7a£) 2-Pyridin-3-ylthiazole-4-carboxylic acid [3-methyl-15-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl) butyl]amide HPLC Rt = 12.55niins (> 80%), HPLC-MS 443.2 [M + H]+. EXAMPLE 96. (3aS, 7aR) Biphenyl-4-carboxylic acid [3-methyl-15-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl)butyl]amide EXAMPLE 97. (3aS, 7aR) 4-/ert-butyl-^-[3-mefliyl-15-(3-oxo-hexahydiofuro[3,2- 6]pyridine-4-carbonyl) butyl]benzamide HPLC Rt = 20.42mins (> 80%), HPLC-MS 415.2 [M + H]+. EXAMPLE 98. (3aS, 7aR) JV-[3-meayl-15-(3-oxo-hexahydK)fuio[3^-&]pyridine^-carbonyl)butyl]-4-thiophen-2-ylbenzamide (Figure Remove)HPLC Rt = 19.60mins (> 80%), HPLC-MS 441.2 [M + H]+. EXAMPLE 99. (3aS, 7aK) 7V-[3-methyl-15'-(3-oxo-hexahydrofuro[352-i]pyridine-4-carbonyl)butyl]-4-trifluoromethoxybenzamide (Figure Remove)- HPLC Rt = 19.66mins (> 80%), HPLC-MS 443.2 [M + H]+. EXAMPLE 100. (3aS; laK) 2-Methyl-5-phenylfuran-3-carboxylic acid [3-methyl- 1 iS-^-oxo-hexahydrofurop ,2-i]pyridine-4-carbonyl) butyljamide HPLC Rt = 20.48mins (> 80%), HPLC-MS 439.2 [M + H]+. EXAMPLE 101. (3aS, 7aR) Moipholine-4-carboxyUc acid [lS-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]amide HPLC-MS 418.2 [M + Hf. EXAMPLE 102. (3aS, laK) JV-[15-(4-hydroxybenzyl-2-oxo-2-(3-oxo-hexahydroforo [3,2-6]pyridin -4-yl)ethyl]benzamide PLC Rt = 12.71mins (>85%), HPLC-MS 409.2 [M + H]+. EXAMPLE 103. (3a5, laK) Naphthalene-1-carboxylic acid [15-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydroftjro[3,2-fe]pyridin-4-yl)ethyl]amide HPLC Rt = 14.88mins (>85%), HPLC-MS 459.2 [M + H]+. HPLC Rt = 11.14mins (>85%), HPLC-MS 399.2 [M + H]+. EXAMPLE 104. (3aS, laK) Furan-3-carboxylic acid [15-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydroiuro[3,2-^]pyridin-4-yl)ethyl]amide EXAMPLE 105. (3a5, laK) Benzo[fc]thiophene-2-carboxylic acid [15'-(4-hydroxy ben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridm-4-yl)ethyl]amide HPLC Rt = 15.99mins (>85%), HPLC-MS 465.2 [M + H]+. EXAMPLE 106. (3aS, 7aR) Benzo[fe]thiophene-3-carboxylic acid [15-(4-hydroxy benzyl)-2-oxo-2-(3-oxo-hexah.ydrofuro[3,2-6]pyridin-4-yl)eihyl]amide (Figure Remove)HPLC Rt = 15.58mins (>85%), HPLC-MS 465.2 [M + H]+5 951.3 [2M +Naf. EXAMPLE 107. (3aS, laK) Furan-3-carboxylic acid [15'-benzyl-2-oxo-2-(3-oxo-hexahydrofuro[352-^]pyridin-4-yl)ethyl]amide (Figure Remove) HPLC Rt = 14.44mins (>85%), HPLC-MS 383.2 [M + Hf. EXAMPLE 108. (3aS, 7 a/?) Thiophene-3-carboxylic acid [15-benzyl-2-oxo-2-(3-oxo-hexah.ydrofuro[3,2-6]pyridin-4-yl)ethyl]amide HPLC Rt = 15.34mins (>80%), HPLC-MS 399.2 [M + Hf. EXAMPLE 109. (3aS, 7aK) Morpholine-4-carboxylic acid [3-methyl-15-(3-oxo-hexa hydrofuro[3,2-6]pyridine-4-carbonyl)butyl]amide HPLC-MS 368.2 [M + Hf. EXAMPLE 110. (3aS, 7a^)^-[3-methyl-15-(3-oxo-hexahydrofuro[3,2-i]pyridine-4-carbonyl) butyl]benzamide HPLC Rt = 15.42mins (>85%), HPLC-MS 359.2 [M + H]+. EXAMPLE 111. (3aS, laK) Furan-3-carboxylic acid [3-methyl-15-(3-oxo-hexahydro furo [3,2-6]pyridine-4-carbonyl) butyl] amide (Figure Remove)HPLC Rt = 13.74mins (>85%), HPLC-MS 349.2 [M + H]+. EXAMPLE 112. (3aS, laK) Benzo[6]tbiophene-2-carboxylic acid [3-methyl-15-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl) butyl] amide HPLC Rt = 18.52mins (>85%), HPLC-MS 415.2 [M + H]+, 851.3 [2M + Naf. EXAMPLE 113. (3aS, 7&K) 4-Dime%larnino-Ar-[3-metliyl-15'-(3-oxo-hexahydro furo[3,2-6]pyridine-4-carbonyl)butyl]ben2amide HPLC Rt = 13.55mins (>85%), HPLC-MS 402.2 [M + H]+. EXAMPLE 114. (3aS, laR) Benzo[6]thiophene-3-carboxylic acid [3-methyl-lS-(3-oxo-hexahydrofuro[3,2-i]pyridine-4-carbonyl)butyl]amide HPLC Rt = 18.26mins (>85%), HPLC-MS 415.2 [M + H]+. EXAMPLE 115. (3aS, laR) Thiophene-3-carboxylic acid Omethyl-lS-^-oxo-hexa liydrofuro[3,2-fe]pyridine-4-carbonyl)butyl]amide HPLC Rt = 14.80mins (>85%), HPLC-MS 365.2 [M + Hf. EXAMPLE 116. (3aS, laR) N-[lR-ber^ls^lfanylme±y\-2-oxo-2-(3-oxo-'hexahydTO furo[3,2-Z)]pyridin-4-yl)ethyl]benzamide HPLC Rt = 17.78mins (>90%), HPLC-MS 439.2 [M + H]+, 899.3 [2M + Na]+. EXAMPLE 117. (3aS, 7aJ?) Furan-3-carboxylic acid-[l^-benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyridin-4-yl)ethyl]amide HPLC Rt = 16.41mins (>85%), HPLC-MS 429.2 [M + H]+, 451.2 [M + Na]+. EXAMPLE 118. (3aS, 7aJ?) Thiophene-3-carboxylic acid-[l/?-ben2ylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyridin-4-yl)ethyl]amide HPLC Rt = 17.23mins (>85%), HPLC-MS 445.2 [M + H]+, 911.2 [2M + Naf. EXAMPLE 119. (3aS, 7a/J) Morpholine-4-carboxylic acid [l.S-cyclohexylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyridin-4-carbonyl)ethyl]amide (Figure Remove) HPLC-MS 408.3 [M + Hf, 430.3 [M + Naf. EXAMPLE 120. (3aS, laR) W-[lS-cyclohexylme%l-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyridin-4-yl)ethyl]benzamide HPLC Rt = 18.47mins (>85%), HPLC-MS 399.2 [M + H]+, 819.2 [2M + Naf. EXAMPLE 121. (3aS, 7aK) Furan-3-carboxylic acid [l (Figure Remove)HPLC Rt = 17.12mins (>85%), HPLC-MS 389.2 [M + Hf, 411.2 [2M + Naf. EXAMPLE 122. (3aS, 7aK) Thiophene-3-carboxylic acid [15'-cyclohexylmethyl-2-oxo-2-(3-oxo-hexah.ydroforo[3,2-^]pyridin-4-yl)ethyl]amide HPLC Rt = 17.95mins (>85%), HPLC-MS 405.2 [M + Hf, 831.2 [2M + Naf. EXAMPLE 123. (3aS, 7 aft) Furan-3-carboxylic acid [15-cyclopentylmethyl-2-oxo-2-(3-oxo-hexab.ydrofuro[3,2-fo]pyridin-4-yl)ethyl]amide HPLC Rt = 1 S.SOmins (>80%), HPLC-MS 375.2 [M + Hf, 771.3 [2M + Naf. EXAMPLE 124. (3aS, 7aR) Thiophene-3-carboxylic acid [15-cyclopentylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]amide HPLC Rt = 16.72mins (>85%), HPLC-MS 391.1 [M + Hf, 803.3 [2M + Naf. EXAMPLE 125. (3aS, laR) Fioran-3-carboxylic acid [3J3-dimethyl-l1S'-(3-oxo-liexa hydrofuro[3,2-Z?]pyridine-4-carbohyl)butyl]amidePLC Rt = 15.06mins (>90%), HPLC-MS 363.2 [M + Hf, 747.4 [2M + Naf. EXAMPLE 126. (3aS, laK) Thiophene-3-carboxylic acid [3,3-dimethyl-15'-(3-oxo-hexahydrofuro[3,2-&]pyridine-4-carbonyl) butyl]amide HPLC Rt = 15.75mins (>85%), HPLC-MS 379.2 [M + Hf, 779.3 [2M + Naf. EXAMPLE 127. (3aS, 7aR) Morpholine-4-carboxylic acid 15-cyclohexyhnethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyridin-4-yl)ethyl ester HPLC-MS 409.2 [M + Hf, 839.5 [2M + Naf. As detailed for EXAMPLE 74, compound (33) was coupled under standard conditions (following Fmoc removal) to the (3aS, laK) 3-oxo-hexahydrofuro[3,2-£]pyridine-4-carboxylic acid 9#-fluoren-9-ylmethyl ester equivalent of loaded building block-linker construct (22), then cleaved to provide EXAMPLE 127. EXAMPLE 128. (2S, 3aS, 7aR) 4-[3-Cyclohexyl-2-(furan-2-yknethanesulfanyl) propionyl]hexahydrofuro[3,2-6]pyridin-3-one HPLC-MS 392.1 [M + Hf, 805.2 [2M + Na]+. As detailed for EXAMPLE 76, compound (41) was coupled under standard conditions (following Fmoc removal) to the (3aS, laR) 3-oxo-hexahydrofuro[3,2-&]pyridine-4-carboxylic acid 9.fiT-fiuoren-9-ylmetb.yl ester equivalent of loaded building block-linker construct (22), then cleaved to provide EXAMPLE 128. EXAMPLE 129. (2S, 3aS, laR) 4-[3-Cyclohexyl-2-(furan-2-yhnethanesulphonyl) propionyl]hexahydrofuro[3,2-fc]pyridin-3-one HPLC Rt = 19.015mins (>%), HPLC-MS 424.1 [M + Hf, 869.2 [2M + Naf. As detailed for EXAMPLE 76, compound (41) was coupled under standard conditions (following Fmoc removal) to the (3aS, laR) 3-oxo-hexahydrofuro[3,2-£»]pyridine-4-carboxylic acid 9/J-fluoren-9-ylmethyl ester equivalent of loaded building block-linker construct (22). The intermediate loaded thioether (1.2p,mole gear) was oxidised with m-chloroperbenzoic acid (5eq, 65% reagent, 1.6mg) in dichloromethane (200faL) for 5hrs, followed by standard washing and then cleaved to provide EXAMPLE 129. EXAMPLE 130. (3aS, 7aR) 2tf-Cyclohexylmethyl-4-morpholin-4-yl-l-(3-oxo-hexa hydrofuro[3,2-&] pyridin-4-yl)butane-l ,4-dione HPLC-MS 407.1 [M + Hf, 833.3 [2M+Naf. As detailed for EXAMPLE 77, compound (36) was coupled under standard conditions (following Fmoc removal) to the (3aS, 7a/?) 3-oxo-hexahydrofuro[3,2-6]pyridine-4-carboxylic acid 9#-fluoren-9-ylmethyl ester equivalent of loaded building block-linker construct (22), then cleaved to provide EXAMPLE 130. EXAMPLE 131 (An example of general formula (II)). (3aR, 1aS) Morpholine-4-carboxylic acid [lJ?-benzylsulfanylmethyl-2-oxo-2-(3-oxohexahydrofuro[3,2-6]pyridin-4-yl)ethyl]amide The required bicycle building block (3aR, laS) 3-oxo-hexahydrofuro[3,2-6]pyridine-4-carboxylic acid 977-fluoren-9-ylmethyl ester was prepared in a further 4 steps from (2R, 3S) (3-hydroxy) pyrrolidine-l,2-dicarboxylic acid 2-allyl ester l-(9H-fluoren-9-ylmethyl) ester as follows. (1) Preparation of (2R, 3S) (3-tert-butoxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester l-(9H-fluoren-9-ylmethyl) ester. (2R, 35) (3-hydoxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester 1-(9H-fluoren-9-ylmethyl) ester (1.75g, 4.30mmole) was dissolved in dry dichloromethane (20mL) in a 50mL glass pressure tube and cooled to -78°C. Isobutylene gas (~10mL) was condensed into the solution and cH2SO4 (100(j.L) added. A stirrer bar was added, the tube was sealed, the cooling removed and stirred at RT for 72hr. The sealed tube was cooled to -78°C, N-methylrnorpholine (200pL, leq w.r.t. cH2SO4) and allowed to warm to RT, unsealed, with stirring over 2hr. Dichloromethane (75mL) was added and the organics washed with NaHCO3 (75mL), then brine (75mL) and dried (NaiSO^. The solvents were removed in vacuo to give a pale tan oil (1.93g). The crude oil was purified over silica gel (lOOg) eluting with a gradient of heptane : ethyl acetate 5:1 •> 3:1. Desired fractions were combined and reduced in vacuo to a thick clear oil yield 1.48g (3.19mmole, 74.3%). TLC (single UV spot, Rf = 0.50, heptane: ethyl acetate 2:1), analytical HPLC Rt = 24.083mins, HPLC-MS (single main UV peak with Rt = 11.996mins, 408.2 [M + H-Bu'f, 486.3 [M + Naf). Analysis by H and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond. 8H (CDC13 at 298K); 1.24 (9 x C(CH3)3, s), 1.51-1.90 (2Hy+ 2H8, m), 3.38-3.48/3.49-3.58 (lHe, dt), 3.62-3.74 (Hp, b), 3.95-4.02/4.06-4.14 (1HE, dd), 4.24-4.78 (Fmoc H-9 + Fmoc CH^ + 2 x COOCH2CH=CH2, bm), 4.88/5.01 (Ha, dd, J5.8), 5.25 (1 x COOCH2CH=CH2, d, J5.7), 5.37 (1 x COOCH2CH=CH2, d, J16.8), 5.88-6.00 (1 x COOCH2CH=CH2, m), 7.28-7.39 (2H aromatic, Fmoc H-2 and H-7), 7.39-7.46 (2H aromatic, Fmoc H-3 and H-6), 7.57-7.71 (2H aromatic, Fmoc H-l and H-8), 7.78-7.86 (2H aromatic, Fmoc H-4 and H-5). 5C (CDC13 at 298K); 23.69/24.00 (d, C5), 28.38 (u, C(CH3)3), 28.65 (d, Cy), 40.41/40.72 (d, Ce), 47.61 (u, Fmoc C-9), 58.46 (u, Ca), 65.67 (d, COOCH2CH=CH2), 67.82 (u, Cp), 68.12 (d, Fmoc CH2), 74.93 (q, C(CH3)3), 118.46/118.57 (d, COOCH2CH=CH2), 120.40 (u, Fmoc C-4 and C-5), 125.39 (u, Fmoc C-l and C-8), 127.46 (u, Fmoc C-2 and C-7), 128.10 (u, Fmoc C-3 and C-6), 132.49 (u, COOCH2CH=CH2), 141.71 (q, Fmoc C-4' and C-5'), 144.12/144.35 (q, Fmoc C-l' and C-8'), 156.24 (q, OCON), 170.42/171.06 (COOCH2CH=CH2). (2) Preparation of (2R, 35) (3-ter^butoxy)pyrrolidine-l,2-dicarboxylic acid 1-(9H-fhioren-9-ylmethyl) ester. (27?,3S) (3-ter^butoxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester 1-(9H-fluoren-9-ylmethyl) ester (1.42g, 3.07mmole) was dissolved in dry dichloromethane (25mL) with stirring. TetraMstriphenylphosphine palladium (0) (71mg, 0.062mmole, 0.02eq) was added, followed by phenyltrihydrosilane (0.66g, 0.581mL, 6.14mmole, 2eq). After Ihr, dichloromethane (150mL) was added and the organics washed with 0.01N HC1 (150mL), brine (150mL) and dried (Na2SO4). The solvents were removed in vacuo to give a dark grey solid (2.0g). The crude solid was purified over silica gel (75g) eluting with a gradient of heptane : ethyl acetate 2:1 -^ 1:2. Desired fractions were combined and reduced in vacuo to a white crystalline yield 1.07g (2.53mmoles 82.3%). TLC (single UV spot, Rf = 0.25, heptane : ethyl acetate 1:1), analytical HPLC Rt = 21.306mins, HPLC-MS (single main UV peak with Rt = 10.269mins5 368.2 [M + H-Bu'f, 446.2 [M + Naf, 869.3 [2M + Naf). Analysis by H and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond. sH (CDC13 at 298K); 1.32/1.41 (9 x C(CH3)3, ds), 1.43-1.59 (1HY+ 1H5, m), 1.70-1.81 (1H8, bm), 1.82-1.99 (1HY, bm), 2.75-2.82/2.90-2.97 (!He, dt), 3.73-3.85 (Hp, b), 3.92-4.05 (lHe, dd), 4.23-4.32 (Fmoc H-9), 4.40-4.58 (Fmoc CH2, bm), 4.60-4.62/5.02-5.04 (Ha, dd, J5.7), 7.29-7.36 (2H aromatic, Fmoc H-2 and H-7), 7.39-7.44 (2H aromatic, Fmoc H-3 and H-6), 7.54-7.67 (2H aromatic, Fmoc H-l and H-8), 7.77-7.82 (2H aromatic, Fmoc H-4 and H-5), 10.80-11.15 (COOH,bs). 5C (CDC13 at 298K); 23.71/24.24 (d, C5), 28.20/28.27 (u, C(CH3)3), 30.60/30.73 (d, CY), 40.50/40.79 (d, CE), 47.58/47.63 (u, Fmoc C-9), 58.20/58.44 (u, Ca), 67.77/68.33 (u, Cp), 68.67/68.87 (d, Fmoc CH2), 78.48/78.67 (q, C(CH3)3), 120.30 (u, Fmoc C-4 and C-5); 125.16/125.27/125.52 (u, Fmoc C-l and C-8), 127.42/127.48/127.59 (u, Fmoc C-2 and C-7), 128.04/128.10 (u, Fmoc C-3 and C-6), 141.57/141.69/141.78 (q, Fmoc C-4' and C-51), 144.06/144.11/144.36/144.47 (q, Fmoc C-l' and C-8'), 155.73/156.49 (q, OCON), 169.26/169.55 (COOH). (3) Preparation of (2R, 35) (3-tert-butoxy)-2-(2-diazoacetyl)pyrrolidine-l,2-dicarboxylic acid l-(9H-fluoren-9-ylmethyl) ester. (2R, 3S) (3-te7t-butoxy)pyrrolidine-l,2-dicarboxylic acid l-(9H-fluoren~9-ylmethyl) ester (830mg, 1.96mmole) was dissolved with stirring in dry dichloromethane (20mL). The reaction was flushed with nitrogen and cooled to -15°C. Isobutylchloroformate (296mg, 2.16mrnole in dry dichloromethane (2.5mL) and N-methylmorpholine (397mg, 3.92mmole in dry dichloromethane (2.5mL) were added simultaneously in O.SmL aliquots over ISmins. Etheral diazomethane (generated from diazald (2.5g, ~8mmole in diethyl ether (40mL)) onto sodium hydroxide (2.75g) in water (4.3mL) / ethanol (8.6mL) at 60°C). was added to the activated aminoacid solution and stirred at RT for 3hr, Acetic acid (~3mL) was added to quench the reaction, then terNbutylmeraylether (lOOmL) was added, the organics washed with water (3 x 150mL), then dried (NaaSCU). The solvents were removed in vacuo to give a tacky pale yellow oil (910mg). The crude oil was purified over silica gel (75g) eluting with a gradient of heptane : ethyl acetate 4:1 -^ 3:1. Desired fractions were combined and reduced in vacuo to a pale yellow oil/solid, yield 560mg (1.25mmole, 63.9%). TLC (single main UV spot, Rf= 0.35, heptane : ethyl acetate 2:1), analytical HPLC Rt = 18.754min, HPLC-MS (UV peaks with Rt = 9.095mins, 364.2 [M + H]+, 386.1 [M + Naf, 749.2 [2M + Naf and Rt = 10.856mins, 420.2 [M + H-N2]+5 470.2 [M + Naf, 917.3 [2M + Naf). Note that upon HPLC-MS analysis, m/z 364.2 corresponds to the desired bicycle product (3a#, 7aS) 3-oxo-hexahydrofuro[3,2-6]pyridine-4-carboxylic acid 9#-fluoren-9-ylmethyl ester. Analysis by 1H and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond. 5H (CDC13 at 298K); 1.29/1.46 (9 x C(CH3)3, ds), 1.40-1.85 (2HY+ 2H55bm), 3.05/3.38 (1HE, dt), 3.39-3.44/3.62-3.69 (Hp, dm), 3.90-3.97 (1HE) dd), 4.05-4.07/4.78-4.83 (Ha, bin), 4.24-4.33 (Fmoc H-9), 4.36-4.42/4.46-4.51 (1 x Fmoc CH2, dm), 4.61-4.68 (1 x Fmoc CFjb, m), 4.89/5.90 (COCH=N2, s + bs), 7.28-7.39 (2H aromatic, Fmoc H-2 and H-7), 7.40-7.44 (2H aromatic, Fmoc H-3 and H-6), 7.53-7.67 (2H aromatic, Fmoc H-l and H-8), 7.76-7.81 (2H aromatic, Fmoc H-4 and H-5). 5C (CDC13 at 298K); 23.94/24.51 (d, C5), 28.38/28.53 (u, C(CH3)3), 29.23/29.84 (d, CY), 40.83/41.45 (d, CE), 47.57/47.64 (u, Fmoc C-9), 55.50/56.38 (u, COCH=N2), 61.69/62.66 (u, Ca), 66.85/68.20 (d, Fmoc CH2), 69.35/69.61 (u, Cp), 75.11/75.54 (q, C(CH3)3), 120.29/120.32 (u, Fmoc C-4 and C-5), 124.85/125.25/125.53 (u, Fmoc C-l and C-8), 127.46/127.59 (u, Fmoc C-2 and C-7), 127.97/128.03/128.07 (u, Fmoc C-3 and C-6), 141.62/141.69/141.73 (q, Fmoc C-4' and C-5'), 144.24/144.31/144.50/144.61 (q, Fmoc C-l' and C-8'), 155.68/156.89 (q, OCON), 191.90/192.37 (COCH=N2). (4) Cyclisation to (3aJ?, 7aS) 3-oxo-hexahydrofuro[3,2-i]pyridine-4-carboxy lie acid 9/f-fluoren-9-ylmethyl ester. A solution of lithium chloride (494mg, 11.6mmole) in water (3mL) and acetic acid (12mL) was added to (2R, 35) (3-tert-butoxy)-2-(2-diazoacetyl) pyrrolidine-l,2-dicarboxylic acid l-(9H-fluoren-9-ylmethyl) ester (520mg, 1.16mmole). Gas was evolved and the yellow oily solid dissolved over Ihr to give a virtually colourless solution. After 90mins, chloroform (150mL) was added and the organics washed with NaHC03 (2 x 150mL), brine (150mL) and dried (Na2SO4). The solvents were removed in vacuo to give a white crystalline solid (390mg). The crude solid was purified over silica gel (75g) eluting with a gradient of heptane : ethyl acetate 3:1 -> 2:1. Desired fractions were combined and reduced in vacuo to a white crystalline solid, yield 340mg (0.94mmole, 47.7% from starting acid). TLC (single UV spot, Rf = 0.25, heptane : ethyl acetate 2:1), analytical HPLC Rt = 18.563mins, HPLC-MS (single UV peak with Rt = 9.074mins, 364.2 [M + H]+, 386.2 [M + Naf). Analysis by JH and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond. 5H (CDC13 at 298K); 1.20-1.38 (1HY+ 1HS) m), 1.51-1,68 (1HS, bm), 1.96-2.08 (1HY, brn)} 2.40-2.52/2.53-2.67 (1HE, m), 3.87 (COCHjA, d, J16.5), 3.85-3.95 (IHe, b), 4.09 (Fmoc H-9, bt), 4.14 (Hp,b), 4.21-4.46 (Fmoc CHz + COCH2B, bm), 4.62-4.70/5.00-5.09 (Ha, 2 x b), 7.23-7.27 (2H aromatic, Fmoc H-2 and H-7), 7.32-7.35 (2H aromatic, Fmoc H-3 and H-6), 7.41-7.53 (2H aromatic, Fmoc H-l and H-8), 7.69-7.71 (2H aromatic, Fmoc H-4 and H-5). 8C (CDC13 at 298K); 22.08 (d, C5), 26.61 (d, C^), 41.67 (d, CE), 47.58 (u, Fmoc C-9), 60.54 (u, Ca), 67.55 (d, Fmoc CH^, 68.40 (d, COCH2O), 72.61 (u, Cp), 120.39 (u, Fmoc C-4 and C-5), 125.42 (u, Fmoc C-l and C-8), 127.51 (u, Fmoc C-2 and C-7), 128.14 (u, Fmoc C-3 and C-6), 141.73 (q, Fmoc C-4' and C-5'), 144.16 (q, Fmoc C-l' and C-8'), 156.20 (q, OCON), 211.64 (q, COCH20). Following the general details from Scheme 2, the required bicycle building block (3a#, 7aS) 3-oxo-hexahydroforo[3^-6]pyridine-4-carboxylic acid 9#-fluoren-9-yl methyl ester was converted to building block-linker construct as follows: (3a#, 7a5) 3-oxo-hexahydrofuro[3,2-6]pyridine-4-carboxylic acid 9#-fluoren-9-ylmethyl ester (290mg, O.SOmmole) was dissolved in a mixture of ethanol (14.0mL) and water (2.0mL) containing sodium acetate.trihydrate (163mg, 1.198mmole, l.Seq). 4-[[(hydrazinocarbonyl)amino]methyl]cyclo hexanecarboxylic acid. trifluoroacetate (263mg, O.SOmmole, l.Oeq, Murphy, A. M. et al, J. Am. Chem, Soc., 114, 3156-3157, 1992) was added and the mixture refluxed for 4hrs. Chloroform (150mL) was added and the organics washed with HC1 (150mL, ~ pH3), dried (Na2S04) and reduced in vacuo to provide crude building block-linker construct as an off-white crystalline solid. Yield 450mg, analytical HPLC 2 peaks Rt = 17.109 (67.6%) and 18.565mins (26.3%) (cis / trans geometrical isomers), HPLC-MS (2 x mins, 561.2 [M + Hf). Crude construct was used directly for construct loading. Following the general details from Scheme 2, the required building block-linker construct was attached to the solid phase providing loaded building block-linker construct as follows: Building block-linker construct (0.680mmoles), 2-(lH-benzotriazole-l-yl)-l, 1,3,3-tetramethyluroniumhexafluoro phosphate (HBTU, 258mg, 0.680mmole), 1-hydroxybenzotriazole.hydrate and (HOBT, lOSmg, 0.680mmole) were dissolved in dimethylformamide (3mL) and N-methylmorpholine (NMM, ISOjiL, 1.36mmole) added. After pre-activation for 5 minutes, free amine gears (190 x 1.2umole) were added, followed by dimethylformamide (17.5mL) and left overnight. The spent coupling solution was then added to free amine crowns (18 x lOfimole) and left overnight. Standard washing and analyses indicated quantitative loading in both cases. Following the general details from Scheme 2, the required loaded building block-linker construct was elaborated on the solid phase as follows: Loaded construct was elaborated to EXAMPLE 91 (3>aR, laS) Morpholine-4-carboxylic acid [lJ?-benzylsulfanyhnethyl-2-oxo-2-(3-oxohexahydrofuro[3,2-Z)] pyridin-4-yl)ethyl]amide by standard Fmoc deprotection and sequential coupling with Fmoc-Cys(SBzl)-OH then morpholine-4-carbonylchloride. The crude example was cleaved and analysed (see general techniques). HPLC Rt = 15.59mins (>80%), HPLC-MS 449.2 [M + Hf. The following examples (132 - 156) were prepared as detailed for EXAMPLE 131, coupling with the required reagents to provide the full length molecule. EXAMPLE 132. (3a#, 7aS) Morpholine-4-carboxylic acid [lS-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-b]pyridin-4-yl)ethyl]amide (Figure Remove)HPLC-MS 418.2[M + Hf. EXAMPLE 133. (3aR, 7aS) J\r-[l.S-(4-hydroxybenzyl-2-oxo-2-(3-oxo-hexahydroriiro [3,2-fc]pyridin-4-yl)ethyl]benzamide HPLC Rt = 12.67mins (>80%), HPLC-MS 409.1 [M + H]+. EXAMPLE 134. (3a#, 7aS) Naphthalene-1-carboxylic acid [15-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydroruro[3,2-6]pyridin-4-yl)ethyl]amide (Figure Remove)HPLC Rt = 14.87mins (>80%), HPLC-MS 459.2 [M + H]+. EXAMPLE 135. (3a/?, 7aS) Morpholine-4-carboxylic acid [3-methyl-15'-(3-oxo-liexa hydrofuro[3,2-Z?]pyridine-4-carbonyl)butyl]arnide HPLC-MS 368.2 [M + H]+, 390.2 [M + Naf. EXAMPLE 136. (3aR, laS) ^-[3-methyl-15-(3-oxo-hexahydrofuro[3,2-fe]pyridine-4-carbonyl) butyl]benzamide (Figure Remove)HPLC Rt = 15.40mins (>80%), HPLC-MS 359.2 [M + H]+. EXAMPLE 137. (3aR, 7aS) Furan-3-carboxylic acid [3-methyl-15-(3-oxo-hexahydro furo[3,2-6]pyridine-4-carbonyl)butyl]amide HPLC Rt = 13.74mins (>85%), HPLC-MS 349.2 [M + Hf, 371.2 [2M + Naf. EXAMPLE 138. (3aR, laS) Benzo[&]tbiophene-2-carboxylic acid [3-methyl-15-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl) butyl]amide (Figure Remove)HPLC Rt = 18.52mins (>80%), HPLC-MS 415.2 [M + H]+, 851.3 [2M + Naf. EXAMPLE 139. (3aR, laS) 4-Dimethylamino-^-[3-inethyl-15-(3-oxo-hexahydro furo[3,2-fo]pyridine-4-carbonyl)butyl]benzamide HPLC Rt = 13.55mins (>80%), HPLC-MS 402.2 [M + Hf, 825.3 [2M + Naf. EXAMPLE 140. (3a#, 7aS) Benzo[6]thiophene-3-carboxylic acid [3-methyl-l,S'-(3-oxo-hexahydrofuro[3,2-6]pyridme-4-carbonyl)butyl]amide HPLC Rt = 18.26mins (>80%), HPLC-MS 415.2 [M + Hf, 851.3 [2M + Naf. EXAMPLE 141. (3a#, 7aS) Thiophene-3-carboxylic acid [3-methyl-lS-(3-oxo-hexa hydroforo[3,2-6]pyridine-4-carbonyl)butyl]amide (Figure Remove)HPLC Rt = 14.80mins (>80%), HPLC-MS 365.2 [M + Hf, 387.2 [M + Naf. EXAMPLE 142. (3aR, 7a5) N-[l^-benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydro furo [3,2-6]pyridin-4-yl)ethyl]benzamide HPLC Rt = 17.78mins (>80%), HPLC-MS 439.2 [M + H]+, 899.3 [2M + Naf. EXAMPLE 143. (3a/?, 7aS) Furan-3-carboxylic acid-tl.R-benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyridin-4-yl)ethyl]amide- HPLC Rt = 16.41mins (>85%), HPLC-MS 429.6 [M + H]+, 451.5 [M + Naf. EXAMPLE 144. (3aR, laS) Thiophene-3-carboxylic acid-fljR-benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]amide HPLC Rt = 17.23mins (>SO%), HPLC-MS 445.1 [M + Hf, 91 1.2 [2M + Naf. EXAMPLE 145. (3a^, 7aS) Morpholine-4-carboxylic acid [15-cyclohexylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)e1:hyl]ainide HPLC-MS 408.2 [M + Hf. EXAMPLE 146. (3a#, 7aS) 7/-[l,S-cyclohexylmethyl-2-oxo-2-(3-oxo-hexaliydrofiiro [3,2-6]pyridin-4-yl)ethyl]benzamide HPLC Rt = 18.47mins (>80%), HPLC-MS 399.2 [M + H]+, 819.3 [2M +Na]+. EXAMPLE 147. (3aK, 7aS) Furan-3-carboxylic acid [15'-cyclohexylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]amide HPLC Rt = 17.12mins (>80%), HPLC-MS 389.2 [M + Hf. EXAMPLE 148. (3a#, 7aS) Thiophene-3-carboxylic acid [15-cyclohexylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]arnide HPLC Rt = 18.05mins (>85%), HPLC-MS 405.2 [M + H]+, 831.2 [2M +Naf. EXAMPLE 149. (3aR, 7aS) Furan-3-carboxylic acid [15-cyclopentylmethyl-2-oxo-2-(3-oxo-hexahydrofaro[3J2-ft]pyridin-4-yl)ethyl]amide HPLC Rt = 15.84mins (>85%), HPLC-MS 375.2 [M + Hf, 771.3 [2M +Naf. EXAMPLE 150. (3aR, laS) Thiophene-3-carboxylic acid [15-cyclopentylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyridin-4-yl)ethyl]amide HPLC Rt = 16.78mins (>85%), HPLC-MS 391.1 [M + Hf, 803.3 [2M EXAMPLE 151. (3aR, laS) Furan-3-carboxylic acid [3,3-dimethyl-l.Sl-(3-oxo-liexa hydrofuro[3,2-6]pyridine-4-carbonyl)butyl]amide HPLC Rt = 15.05mins (>85%), HPLC-MS 363.2 [M + Hf, 747.4 [2M + Naf. EXAMPLE 152. (3a#, 7aS) Thiophene-3-carboxylic acid [3,3-dim.ethyl-15-(3-oxo-hexahy droftiro [3,2-Z?]pyridine-4- carbonyl) butyl] amide HPLC Rt = 15.76mins (>80%)3 HPLC-MS 379.2 [M + H]+, 779.3 [2M + Naf. EXAMPLE 153. (3aR, 7aS) Morpholine-4-cafboxylic acid lS-cyclohexylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-i]pyridin-4-yl)ethyl ester HPLC-MS 409.2 [M + Hf, 839.5 [2M + Naf. As detailed for EXAMPLE 127, compound (33) was coupled under standard conditions (following Fmoc removal) to the (3aR, 7aS) 3-oxo-hexahydrofuro[3,2-6]pyridine-4-carboxylic acid 9//'-fluoren-9-ylmethyl ester equivalent of loaded building block-linker construct (22), then cleaved to provide EXAMPLE 153. EXAMPLE 154 (25, 3aR, laS) 4-[3-Cyclohexyl-2-(furan-2-ylmethanesulfanyl) propionyl]hexahydrofuro[3,2-6]pvridin-3-one HPLC-MS 392.1 [M + H]+, 805.3 [2M + Naf. As detailed for EXAMPLE 128, compound (41) was coupled under standard conditions (following Fmoc removal) to the (3aft, 7aS) 3-oxo-hexahydrofuro[3,2- &]pyridine-4-carboxylic acid 9Muoren-9-ylmethyl ester equivalent of loaded building block-linker construct (22), then cleaved to provide EXAMPLE 154. EXAMPLE 155. (25, 3aR, laS) 4-[3-Cyclohexyl-2-(furan-2-ylmethanesulphonyl) propionyl]hexahydrofuro[3,2-&]pyridin-3 -one HPLC Rt = 18.8 - 19.4mins (>90%), HPLC-MS 424.1 [M + Hf, 869.2 [2M + Naf. As detailed for EXAMPLE 129, compound (41) was coupled under standard conditions (following Fmoc removal) to the (3aR, 7aS) 3-oxo-hexahydrofuro[3,2-i]pyridine-4-carboxylic acid 9#-fluoren-9-ylmethyl ester equivalent of loaded building block-linker construct (22). The intermediate loaded thioethef (1.2jamole gear) was oxidised with m-chloroperbenzoic acid (5eq, 65% reagent, 1.6mg) in dichloromethane (200uL) for 5hrs, followed by standard washing and then cleaved to provide EXAMPLE 155. HPLC-MS 407.2 [M + Hf, 833.3 [2M + Naf. EXAMPLE 156. (3aR, laS) 2J?-Cyclohexyhnethyl-4-morpholin-4-yl-l-(3-oxo-hexa hydrofuro[3,2-6] pyridin-4-yl)butane-l ,4-dione As detailed for EXAMPLE 130, compound (36) was coupled under standard conditions (following Fmoc removal) to the (3aR, 7aS) 3-oxo-hexahydrofuro[3,2-&]pyridine-4-carboxylic acid 9#-fluoren-9-ylmethyl ester equivalent of loaded building block-linker construct (22), then cleaved to provide EXAMPLE 156. EXAMPLE A. Assays for Cysteine Protease Activity The compounds of this invention may be tested in one of a number of literature based biochemical assays that are designed to elucidate the characteristics of compound inhibition. The data from these types of assays enables compound potency and the rates of reaction to be measured and quantified. This information, either alone or in combination with other information, would allow the amount of compound required to produce a given pharmacological effect to be determined. General materials and methods Unless otherwise stated, all general chemicals and biochemicals were purchased from either the Sigma Chemical Company, Poole, Dorset, U.K. or from Fisher Scientific UK, Loughborough, Leicestershire, U.K. Absorbance assays were carried out in flat-bottomed 96-well plates (Spectra; Greiner Bio-One Ltd., Stonehouse, Gloucestershire, U.K.) using a SpectraMax PLUS384 plate reader (Molecular Devices, Crawley, U.K.). Fluorescence high throughput assays were carried out in either 384-well microtitre plates (Corning Costar 3705 plates, Fisher Scientific) or 96-well CU' bottomed Microfluor Wl microtitre plates (Thermo Labsystems, Ashford, Middlesex, U.K.). Fluorescence assays were monitored using a SpectraMax Gemini fluorescence plate reader (Molecular Devices). For substrates employing either a 7-amino-4-methylcoumarin (AMC) or a 7-amino-4-nifluoromethylcoumarin (AFC) fluorophore, assays were monitored at an excitation wavelength of 365 nm and an emission wavelength of 450 nm and the fluorescence plate reader calibrated with AMC. For substrates employing a 3-amino-benzoyl (Abz) fluorophore, assays were monitored at an excitation wavelength of 310 nm and an emission wavelength of 445 nm; the fluorescence plate reader calibrated with 3-amino-benzamide (Fluka). Unless otherwise indicated, all the peptidase substrates were purchased from Bachem UK, St. Helens, Merseyside, UK. Substrates utilizing fluorescence resonance energy transfer methodology (i.e. FRET-based substrates) were synthesized at Incenta Limited using published methods (Atherton & Sheppard, Solid Phase Peptide Synthesis, IRL Press, Oxford, U.K., 1989) and employed Abz (2-aminobenzoyl) as the fluorescence donor and 3-nitro-tyrosine [TyrCNOa)] as the fluorescence quencher (Meldal, M. and Breddam, K.,Anal. Biochem., 195,141-147, 1991). Hydroxyethylpiperazine ethanesulfonate (HEPES), tris-hydroxylro ethyl aminomethane (tris) base, bis-tris-propane and all the biological detergents (e.g. CHAPS, zwittergents, etc.) were purchased from CN Biosciences UK, Beeston, Nottinghamshire, U.K. Glycerol was purchased from Amersham Pharmacia Biotech, Little Chalfont, Buckinghamshire, U.K. Stock solutions of substrate or inhibitor were made up to 10 mM in 100 % dimethylsulfoxide (DMSO) (Rathburns, Glasgow, U.K.) and diluted as appropriately required. In all cases the DMSO concentration in the assays was maintained at less than 1% (vol./vol.). Assay protocols were based on literature precedent (Tablel; Barrett, A.J., Rawlings, N.D. and Woessner, J.F., 1998, Handbook of Proteolytic Enzymes, Academic Press, London and references therein) and modified as required to suit local assay protocols. Enzyme was added as required to initiate the reaction and the activity, as judged by the change in fluorescence upon conversion of substrate to product, was monitored over time. All assays were carried out at 25±1°C. Table 1. The enzyme assays described herein were earned out according to literature precedents. I: 10 mM BTP, pH 6.5 containing 1 mM EDTA, 5 mM 2-mercaptoethanol and 1 mM CaCl2 H: 10 mM BTP, pH 6.5 containing 1 mM EDTA, 142 mM NaCl, 1 mM DTT, 1 mM CaCl2, 0.035 mM Zwittergent 3-16 III: 50mM HEPES pH 7.2, 10% Glycerol, 0.1% CHAPS, 142 mM NaCl, 1 mM EDTA, 5 mM DTT TV: 100 mM sodium phosphate, pH 6.75 containing 1 mM EDTA and 10 mM L- cysteine V: 50 mM trisacetate, pH 8.4 containing 1 mM EDTA, 10 mM L-cysteine and 0.25% (w/v) CHAPS VI: 10 mM HEPES, pH 8.0 containing 5 mM CaCl2 VII: 10 mM HEPES, pH 7.5 containing 2 mM 2-mercaptoethanol and 100 p,M CaCl2 VHI: 10 mM HEPES, pH 7.5 containing 2 mM 2-mercaptoethanol and 200 uM CaCl2 EX: 100 mM sodium acetate; pH 5.5 containing 10 mM L-cysteine and 1 mM EDTA X: 100 mM sodium acetate; pH 5.5 containing 10 mM L-cysteine; 0.05% (w/v) Brij 35 and 1 mM EDTA XI: 100 mM sodium acetate; pH 5.5 containing 10 mM L-cysteine; 142 mM sodium chloride and 1 mM EDTA a Barrett, A.J., Biochem. J., 187,909-912,1980 b Barrett, AJ. and Kirschke, H., Methods Erizymol, 80, 535-561,1981 0 Quibell, M. and Taylor, S.5 WO0069855, 2000 dBromme, D., Steinert,., Freibe, S., Fittkau, S., Wiederanders, B., and Kirschke, H., Biochem. J., 264,475-481,1989 °Rano, T.A., et. al.Chem. Biol, 4,149,1997 fTalanian, R.V., et. al, J. Biol. Chem., 272, 9677,1997 8 Lazebnik, Y.A., Kaufinann, S.H., Desnoyers, S., Poirer, G.G. and Earnshaw, W.C., Nature., 371, 768-774,1994 hHan, Z., et. al., J. Biol. Chem., 272,13432,1997 1 Takahashi, A., et. al, PNAS, 93, 8395,1996 j Martins, L.M., et. al, J. Biol Chem., 272, 7421,1997 k Nagata, S., Cell, 88,355,1997 1 Harris, J.L., et. al, J. Biol. Chem., 273,27364,1998 m Cazzulo, J.J., Cazzulo Franke, M.C., Martinez, J. and Franke de Cazzulo, B.M., Biochim. Biophys. Acta., 1037.186-191,1990 n Cazzulo, J.J., Bravo, M., Raimondi, A., Engstrom, U., Lindeberg, G. and Hellman, U., CellMol Biol, 42, 691-696,1996 0 Potempa, J., Dubin, A., Korzus, G. and Travis, J., Biochem. J., 263, 2664-2667, 1988 p Kembhavi, A.A., Buttle, D J., Rauber, P. and Barrett, A.J., FEES Lett., 283, 277- 280,1991 q Alves, L.C., et. al.,Mol Biochem. Parasitol, 116,1-9, 2001. r Guarne, et.al, J. Mol. BioL, 302,1227-1240,2000. 5 Halfon and Craik, (Barret, Rawlings and Woessner, eds.), in Handbook of Proteolytic Enzymes, Academic Press, London, 12-21,1998. 1 Sasaki, et. al, (1984), J. BioL Chem., 259, 12489-12494,1984. "Bossard, M.J., et. al.,,J. Biol. Chem., 21,12517-12524,1996 v Santamaria, L, et. al., J. Biol. Chem., 273,16816-16823,1998 w Klemencic, J, et al, Eur.J.Biochem., 267,5404-5412,2000 Tiypanosoma cnizi cruzipain peptidase activity assays Wild-type cruzipain, derived from Trypanosoma ciiizi Dm28 epimastigotes, was obtained from Dr. Julio Scharfstein (Institute de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil). Activity assays were carried out in 100 mM sodium phosphate, pH 6.75 containing 1 mM EDTA and 10 mM L-cysteine using 2.5 nM enzyme. Ac-Phe-Arg-AMC (^Mapp ~ 12 uM) and D-Val-Leu-Lys-AMC (K^ ~ 4 uM) were used as the substrates. Routinely, Ac-FR-AMC was used at a concentration equivalent to jKMapp and D-Val-Leu-Lys-AMC was used at a concentration of 25 uM. The rate of conversion of substrate to product was derived from the slope of the increase in fluorescence monitored continuously over time. Leishmania mexicana cysteine protease B (CPB) peptidase activity assays Wild-type recombinant CPB without the C-terminal extention (i.e. CPB2.8ACTE; Sanderson, S.J., et. al., Biochem. J., 347, 383-388, 2000) was obtained from Dr. Jeremy Mottram (Wellcome Centre for Molecular Parasitology, The Anderson College, University of Glasgow, Glasgow, U.K.). Activity assays were carried out in 100 mM sodium acetate; pH 5.5 containing 1 mM EDTA; 200 mM NaCl and 10 mM DTT (Alves, L.C., et. al, Mol. Biochem. Parasitol, 116, 1-9, 2001) using 0.25 nM enzyme. Pro-Phe-Arg-AMC (£Mapp ~ 38uM) was used as the substrate at a concentration equivalent to JCMapp. The rate of conversion of substrate to product was derived from the slope of the increase in fluorescence monitored continuously over time, Cathepsin peptidase activity assays Bovine cathepsin S, human cathepsin L, human cathepsin H and human cathepsin B were obtained from CN Biosciences. Reconibinant human cathepsin S, human cathepsin K and human cathepsin X were obtained from Dr. Boris Turk (Josef Stefan Institute, Ljubljana, Slovenia). Unless otherwise stated, all peptidase activity assays were carried out in 10 mM bis-tris-propane (BTP), pH 6.5 containing 1 mM EDTA, 5 mM 2-mercaptoethanol and 1 mM CaCl2. Human cathepsin H activity assays were carried out in 10 mM BTP pH 6.5, 142 mM NaCl2, 1 mM CaCl2> 1 mM EDTA, 1 mM DTT, 0.035 mM Zwittergent 3-16, Human cathepsin K assays were carried out in 100 mM sodium acetate; pH 5.5 containing 20 mM L-cysteine and 1 mM EDTA (Bossard, M.J., et. a!., J. Biol Chem., 2_i, 12517-12524,1996). Human cathepsin X assays were carried out in 100 mM sodium acetate; pH 5.5 containing 20 mM L-cysteine; 0.05% (w/v) Brij 35 and 1 mM EDTA (Santamaria, I., et. a/., /. Biol Chem., 273, 16816-16823,1998; Klemencic, J, et aL, Eur. J. Biochem., 267, 5404-5412, 2000). The final enzyme concentrations used in the assays were 0.5 nM bovine cathepsin S, 1 nM cathepsin L, 0.1 nM cathepsin B, 0.25nM Cathepsin K; 1 nM cathepsin X and 10 nM cathepsin H. For the inhibition assays, the substrates used for cathepsin S, cathepsin L, cathepsin B, cathepsin K and cathepsin H were boc-Val-Leu-Lys-AMC (^Mapp « 30 nM), Ac-Phe-Arg-AMC (ATMapp « 20 uM), Z-Phe-Arg-AMC (£Mapp « 40 uM), Z-Leu-Arg-AMC (KMapp ~ 2uM); Bz-Phe-Val-Arg-AMC (&Mapp ~ 150 uM) respectively. In each case the substrate concentration used in each assay was equivalent to the KM&PP- The rate of conversion of substrate to product was derived from the slope of the increase in fluorescence monitored continuously over time. Trypsin peptidase activity assays Human pancreatic trypsin (iodination grade; CN Biosciences) activity assays were carried out in 10 mM HEPES, pH 8.0 containing 5 mM CaCl2 using 0.1 nM trypsin. For the inhibition assays, Z-Gly-Gly-Arg-AMC (^Mapp ~ 84 pM) was used as the substrate at a concentration equivalent to KMPP. The rate of conversion of substrate to product was derived from the slope of the increase in fluorescence monitored continuously over time. Clostripain peptidase activity assays Clostripain (Sigma) activity assays were carried out in 10 mM BTP, pH 6.5 containing 1 mM EDTA, 5 mM 2-mercaptoethanol and ImM CaQ2 using 0.3 nM enzyme. For the inhibition assays, Z-Gly-Gly-Arg-AMC CKMBPP ~ 100 pM) was used as the substrate at a concentration equivalent to XMSPP. The rate of conversion of substrate to product was derived from the slope of the increase in fluorescence monitored continuously over time. Calpain peptidase activity assays Calpain (human erythrocyte p,-calpain and porcine kidney m-calpain; CN Biosciences) activity assays were carried out in 10 mM HEPES, pH 7.5 containing 2 mM 2-mercaptoethanol and CaQ2 using 25 nM of either enzyme (Sasaki, et. al, J. Biol. Chem., 259, 12489-12494, 1984). For n-calpain inhibition assays, the buffer contained 100 uM CaCl2 and Abz-Ala-Asn-Leu-Gly-Arg-Pro-Ala-Leu-Tyr(NO2> Asp-NH2 (KMPP ~ 20 H.M; Incenta Limited) was used as the substrate. For m-calpain inhibition assays, the assay buffer contained 200 \iM CaCl2 and Abz-Lys-Leu-Cys(Bzl)-Phe-Ser-Lys-Gh-Tyr(N02)-Asp-NH2 (£Mapp « 22 \M; Incenta Limited) was used as the substrate. In both cases the substrate concentration employed in the assays was equivalent to the ^Mapp- The rate of conversion of substrate to product was derived from the slope of the increase in fluorescence monitored continuously over time. Extracellular S. aureus V8 cysteine peptidase (staphylopairi) peptidase activity assays S. aureus V8 was obtained from Prof. S. Arvidson, Karolinska Institute, Stockholm, Sweden. Extracellular S. aureus V8 cysteine peptidase (staphylopain) activity assays were carried out using partially purified S. aureus V8 culture supernatant (obtained from Dr. Peter Lambert, Aston University, Birrriingharn, U.K.). Activity assays were carried out in 10 mM BTP, pH 6.5 containing 1 mM EDTA, 5 mM 2-mercaptoethanol and ImM CaCl2 using two-times diluted partially purified extract. For the inhibition assays, Abz-Ile-Ala-Ala-Pro-Tyr(NO2)-Glu-NH2 (£MSPP ~ 117 uM; Incenta Limited) was used as the substrate at a concentration equivalent to KUPP. The rate of conversion of substrate to product was derived from the slope of the increase in fluorescence monitored continuously over time, Foot-and-mouth disease leader peptidase (FMDV-LP) activity assays Recombinant wild-type FMDV-LP was obtained from Dr. Tim Skern (Institut fur Medizinische Biochemie, Abteilung fur Biochemie, Universtat Wien, Wien, Austria). Activity assays were carried out in 50 mM trisacetate, pH 8.4 containing 1 mM EDTA, 10 mM L-cysteine and 0.25% (w/v) CHAPS using 10 nM enzyme. For the inhibition assays, Abz-Arg-Lys-Leu-Lys-Gly-Ala-Gly-Ser-Tyr(N02)-Glu-NH2 (&Mapp ~ 51 uM, Incenta Limited) was used as the substrate at a concentration equivalent to KMPP. The rate of conversion of substrate to product was derived from the slope of the increase in fluorescence monitored continuously over time. Caspase peptidase activity assays Caspases 1-10 were obtained from CN Biosciences or BioVision Inc. (Mountain View, CA, USA) and all assays were carried out in 50mM HEPES; pH 7.2, 10% (v/v) glycerol, 0.1% (w/v) CHAPS, 142 mM NaCl, 1 mM EDTA, 5 mM dithiothreitol (DTT) using 0.1-1 U per assay. For caspase 1, Ac-Leu-Glu-His-Asp-AMC was used as the substrate; for caspase 2, Z-Val-Asp-Val-Ala-Asp-AFC was used as the substrate; for caspase 3, Ac-Asp-Glu-Val-Asp-AMC was used as the substrate; for caspase 4, Suc-Tyr-Val-Ala-Asp-AMC was used as the substrate; for caspase 5, Ac-Leu-Glu-His-Asp-AMC was used as the substrate; for caspase 6, Ac-Val-Glu-Ile-Asp-AMC was used as the substrate; for caspase 7, Ac-Asp-Glu-Val-Asp-AMC was used as the substrate; for caspase 8, Ac-Be-Glu-Thr-Asp-AMC was used as the substrate; for caspase 9, Ac-Leu-Glu-His-Asp-AMC was used as the substrate; for caspase 10, Ac-Ile-Glu-Thr-Asp-AMC was used as the substrate (Nicholson, D.W. and Thornberry, N.A., TIBS, 22, 299-306, 1997; Stermicke, H.R. and Salvesen, G.S., J. Biol Chem., 272(41), 25719-25723, 1997; Talanian, R.V., et. al., J. Biol. Chem., 272(15), 9677-9682, 1997; Wolf, B.B. and Green, D.R., J. Biol. Chem. , 274(29), 20049-20052, 1999). The rate of conversion of substrate to product was derived from the slope of the increase La fluorescence monitored continuously over time. Measurement of the apparent macroscopic binding (Michaelis) constants CKMapp) for substrates The apparent macroscopic binding constant (KMBPP) for each substrate was calculated, from the dependence of enzyme activity as a function of substrate concentration. The observed rates were plotted on the ordinate against the related substrate concentration on the abscissa and the data fitted by direct regression analysis (Prism v 3.02; GraphPad, San Diego, USA) using Equation 1 (Comish-Bowden, A. Fundamentals of enzyme kinetics Portland Press; 1995, 93-128.). In Equation 1 'V is the observed initial rate, 'Fmaxapp' is the observed maximum activity at saturating substrate concentration, '£iviapp' is the apparent macroscopic binding (Michaelis) constant for the substrate, '[S0]' is the initial substrate concentration. Measurement of the inhibition constants The apparent inhibition constant (Kj) for each compound was determined on the basis that inhibition was reversible and occurred by a pure-competitive mechanism. The K\ values were calculated, from the dependence of enzyme activity as a function of inhibitor concentration, by direct regression analysis (Prism v 3.02) using Equation 2 (Cornish-Bowden, A., 1995.). In Equation 2 'vi' is the observed residual activity, 'Fmaxap!" is the observed maximum activity (i.e. in the absence of inhibitor), '^Mapp> is the apparent macroscopic binding (Michaelis) constant for the substrate, e[S]' is the initial substrate concentration, 1K\ is the apparent dissociation constant and '[I]' is the inhibitor concentration. In situations where the apparent dissociation constant (£iapp) approached the enzyme concentrations, the £iapp values were calculated using a quadratic solution in the form described by Equation 3 (Morrison, J.F. Trends Biochem. Sci.,_l, 102-105, 1982; Morrison, J.F. Biochim. Biophys. Act^ 185, 269-286, 1969; Stone, S.R. and Hofsteenge, J. Biochemistry, 25,4622-4628,1986). In Equation 3 'vi' is the observed residual activity, 'F' is the difference between the maximum activity (i.e. in the absence of inhibitor) and minimum enzyme activity, CE0' is the total enzyme concentration, '^Tjapp' is the apparent dissociation constant and '!„' is the inhibitor concentration. Curves were fitted by non-linear regression analysis (Prism) using a fixed value for the enzyme concentration. Equation 4 was used to account for the substrate kinetics, where 'Ki is the inhibition constant, '[S0]' is the initial substrate concentration and 'j5rMapp' is the apparent macroscopic binding (Michaelis) constant for the substrate (Morrison, 1982). The second-order rate of reaction of inhibitor with enzyme Where applicable, the concentration dependence of the observed rate of reaction (^obs) of each compound with enzyme was analysed by determining the rate of enzyme inactivation under pseudo-first order conditions in the presence of substrate (Morrison, J.F., TIBS, 102-105,1982; Tian, W.X. and Tsou, C.L., Biochemistry, 21, 1028-1032, 1982; Morrison, IF. and Walsh, C.T., from Meister (Ed.), Advances in Enzymol., 61, 201-301,1988; Tsou, C.L., from Meister (Ed.), Advances in Enzymol, 6\j 381-436,1988;). Assays were carried out by addition of various concentrations of inhibitor to assay buffer containing substrate. Assays were initiated by the addition of enzyme to the reaction mixture and the change in fluorescence monitored over time. During the course of the assay less than 10% of the substrate was consumed. The activity fluorescence progress curves were fitted by non-linear regression analysis (Prism) using Eq. 5 (Morrison, 1969; Morrison, 1982); where T' is the fluorescence response, 't' is time, 'v0' is the initial velocity, 'vs' is the equilibrium steady-state velocity, 'A:0bs' is the observed pseudo first-order rate constant and 'D' is the intercept at time zero (i.e. the ordinate displacement of the curve). The second order rate constant was obtained from the slope of the line of a plot of &0bS versus the inhibitor concentration (i.e. &0bs/[I]). To correct for substrate kinetics, Eq. 6 was used, where '[S0]' is the iniitial substrate concentration and 'jK"Mapp' is the apparent macroscopic binding (Michaelis) constant for the substrate. Compounds of the invention were tested by the above described assays and observed to exhibit cruzipain inhibitory activity or inhibitory activity against an alternative CA Cl cysteine protease with an in vitro Ki inhibitory constant of less than or equal to 100\|aM. Exemplary inhibition data for a number of example compounds of the invention are given in table 2. (TableRemove) We claim: 1. A compound according to general formula (I) or general formula (II): (Formula Removed) wherein: R1 = C0-7-alkyl (when C = 0, R1 is simply hydrogen). C3-6-cycloaIkyl or Ar- C0-7-alkyl (when C = 0, R1 is simply an aromatic moiety Ar); Z - O or S; P1=CR5R6, P2= CR7R8, Q=CR9R10 or NR11 where R11 is chosen from C0-7-alkyl; each of R2, R3. R5- R6, R7. R8. R9 and R10 is indcpendently chosen from C0-7-alkyl. C3-6-cycloaIkyl, Ar-C0-7-alkyl. O- C0-7,-alkyl. ()-C3.ft-cycloalkyl. O-Ar- C0-7-alkyl. S-C0-7-alkyl S-C3-6-cycloalkyl. S-Ar-C0-7-alkyl. NH- C0-7-alkyl. NH- C3-6-cycloaIkyl, NH-Ar-C0-7-alkyl, N(C0-7-alkyI)2, N( C3-6,.cycloalkyl)2 or N(Ar- C0-7-alkyl):: Y = CRl2Rl3-CO, where R12, R13 are chosen from C0-7-alkyl, C3-6-cycloalkyl and Ar-C0-7-alkyl; in the group (X)o, X =, CR,4RIS, where R14 and R15 are independently chosen from C0-7-alkyl, C3-6-cycloaIkyl and Ar-C0-7-alkyI and O is a number from zero to three; in the group (W)n. W = O, S, C(O), S(O) or S(0)2 or NR16, where R16 is chosen from C0-7-alkyl, C3.6.cycloalkyl and Ar-C0-7-alkyl and n is zero or one; in the group (V)m. V = C(O), C(S), S(O), S(O),. S«))2NH, OC(O), NHC(O), NHS(O), NHS(0)2, OC(0)NH, C(0)NH or CR17R18, where R17 and R18 are independently chosen from C0-7-alkyl, C3.6.cycloalkyl, Ar-C0-7-alkyl and m is a number from zero to three, provided that when m is greater than one, (V)m contains a maximum of one carbonyl or sulphonyl group; U = a 5- to 7-membered monocyclic or a 8- to 1 l-mcmbered bicyclic ring which is selected from the group consisting of: (Formula Removed) wherein R ' is: C0-7-alkyl, C3-6-cycIoalkyI, Ar-C0.7-aIkyl, O-C0-7-aIkyI, 0-C3-6-cycloalkyl, O-Ar-C0-7-alkyl, S-C0-7-aIkyl, S-C3-6-cycloalkyl, S-Ar-C0-7-aIkyl, NH-C0-7-alkyl, NH-C3-6-cycloalkyl, NH-Ar-C0-7-aIkyl, N(C0-7-alkyl)2, N(C3-6.cycloalkyI)2 or N(Ar-C0-7-alkyl)2; or, when part of a CHR19 or CR19 group, R19 may be halogen; A is chosen from: CH2, CHR19,0, S and NR20; where R19 is as defined above; and R20 is chosen from: C0-7-alkyl, C3-6-cycloalkyl and Ar-C0-7-alkyI; B, D and G are independently chosen from: CR19, where R19 is as defined above, or N or N-oxide (N→O); E is chosen from: CH22, CHR10, O, S and NR20, where R19 and R20 arc defined as above; J, L, M, R, T. T2, T3 and T4 are independently chosen from: CR19 and N or N-oxidc (N→0). where R19 is as defined above; T.s is chosen from: CH or N; q is a number from one to three, thereby defining a 5-, 6- or 7-membered ring; and all salts, hydrates, solvates, complexes and prodrugs thereof. 2. A compound as claimed in claim 1 wherein independently or in combination: a) Z is O, b) P1 and P2 arc CH:: c) Q is CH2 or NH; d) R1 is Co-7-alkyI or Ar- Co-7-alkyl; e) Y is CR12R13CO where R12, R13 are selected from C0-7-alkyl, C3-6-cycloalkyl and Ar- C0-7-alkyl; f) in the group (X)0, X is CR14R15 and each of R14 and R15 is selected from C0-7-alkyl and Ar- C0-7-alkyl; g) in the group (W)n. W is O, S, S02, C(O) or NR16. where R16 is C0-4- alkyl; and n is 0 or 1; h) in the group (V)m. V is C(O). C(0)NH or CR18, where R18 is C0-4-alkyl; and m is 0 or 1; i) U is a 5- or 6- membered saturated or unsaturated heterocycle or a saturated or unsaturated 9- or 10- membered heterocycle selected from the structures set out in claim I. 3. A compound as claimed in claim 1 or claim 2 wherein R1 is hydrogen, C1-4alkyl or Ar-C1-4-alkyl. 4. A compound as claimed in anyone of claims 1 to 4 where (X)„- Y is selected from one of the following moieties: (Formula Removed) wherein R19, R2U and Ar are as defined above. 5. A compound as claimed in anyone of claims 1 to 4 wherein Y is CHRl3CO where R is Ar-CH2-, where the aromatic ring is phenyl or monocyclic heterocycle, optionally substituted with an R19 group, wherein R19 is as defined in claim 1; or R13 comprises cyclohexylmethyl. 6. A compound as claimed in anyone of claims 1 to 5 wherein (X)0- Y is selected from the following: (Formula Removed) wherein R19 and Ar are as defined previously 7. A compound as claimed in anyone of claims 1 lo 6, wherein (W)n-(X)0- Y is one of the following moieties: (Formula Removed) 8. A compound as claimed in anyone of claims 1 to 7, wherein in the group (X)0: X is CR14R15, R14 is hydrogen, R15 is C1-4-alkyl; and o is 0 or 1. 9. A compound as claimed in anyone of claims 1 to 8 wherein, in the group (W)n: W is 0, S, SO2 C(O) or NH where n is 0 or 1. 10. A compound as claimed in anyone of claims 1 to 9 wherein the group (W)n is: NH. 11. A compound as claimed in anyone of claims 1 to 16 wherein the combination U-(V)n (W)n-(X)0-Y or U-(V)m-(W)n-(X)0 is one of the following: (Formula Removed) 12. A compound as claimed in anyone of claims 1 to 11 wherein the combination U-(V)n (W)n-(X)0-Y is one of the following: (Formula Removed) 13. A compound as claimed in anyone of claims 1 to 12 wherein U comprises one of the following: (Formula Removed) wherein R19 is as defined previously. 14. A compound as claimed in anyone of claims 1 to 13. wherein U represents a group: (Formula Removed) wherein R19, D, E, G, J, L, M, R, T, T2, T3 and T4 are as defined previously. 15. A compound as claimed in anyone of claims 1 to 14, wherein U represents a group (Formula Removed) wherein R , D, E, G, J, L, M, R and T are as defined previously. 16. A compound as claimed in anyone of claims 1 to 15. wherein U represents a group (Formula Removed) wherein R19 .D. I. G. J and 1. are as defined previously. 17. A method of validating a known or putative cysteine protease as a therapeutic target, the method comprising: (a) assessing the in vitro binding of a compound as claimed in anyone of claims 1 to 16 to an isolated known or putative cysteine protease, providing a measure of'potency'; and optionally, one or more of the steps of: (b) assessing the binding of a compound as claimed in anyone of claims 1 to 16 to closely related homologous proteases of the target and general house-keeping proteases (e.g. trypsin) to provides a measure of'selectivity'; (c) monitoring a cell-based functional marker of a particular cysteine protease activity, in the presence of a compound as claimed in anyone of claims 1 to 16; and (d) monitoring an animal model-based functional marker of a particular cysteine protease activity, in the presence of a compound as claimed in anyone of claims 1 lol6. 18. The use of a compound as claimed in anyone of claims 1 to 16, or a pharmaceutically or veterinarily acceptable salt thereof, in the validation of a known or putative cysteine protease as a therapeutic target. 19. A compound as claimed in anyone of claims 1 to 16, or a pharmaceutically or \eterinarily acceptable salt thereof, for use in medicine. 20. Use of a compound as claimed in anyone of claims. 1 to 16, or a pharmaceutically or veterinarily acceptable salt thereof, in the preparation of a medicament for preventing or treating diseases in which the disease pathology may be modified by inhibiting a cysteine protease. 21. Use of a compound as claimed in anyone of claims 1 to 16, or a pharmaceutically or veterinarily acceptable salt thereof, in the preparation of a medicament for treating infections caused by Pneumocystis carinii, Tryps'anoma cruzi, Trypsanoma brucei, Leishmania Mexicana, Clostridium histolyticum, Staphylococcus aureus, foot-and-mouth disease virus and Crithidia fusiculata; as well as in osteoporosis, autoimmunity, schistosomiasis, malaria, tumour metastasis, metachromatic leukodystrophy, muscular dystrophy and amytrophy. 22. Use of a compound as claimed in anyone of claims 1 to 16, or a pharmaceutical!}' or veterinarily acceptable salt thereof, in the preparation of a medicament for preventing or treating Chagas' disease. 23. Use of a compound as claimed in anyone of claims I to 16, or a pharmaceutical^ or veterinarily acceptable salt thereof, in the preparation of a medicament for treating an autoimmune disorder. 24. Use of a compound as claimed in anyone of claims 1 to 16, or a pharmaceutically or veterinarily acceptable salt thereof, in. the preparation of a medicament for treating osteoporosis. 25. A pharmaceutical or veterinary composition comprising one or mote compounds as claimed in anyone of claims 1 to 16 and a pharmaceutically or veterinarily acceptable carrier. 26. A process for the preparation of a pharmaceutical or veterinary composition as claimed in claim 25, the process comprising bringing the active compound(s) into association with the carrier, for example by admixture.

Full Text

INHIBITORS OF CRUZIPAIN AND OTHER CYSTEINE PROTEASES
T
HIS INVENTION relates to compounds which are inhibitors of the protease cruzipain, a gene product of the Tiypanosoma cruzi parasite. In particular, the invention provides compounds that are useful for the therapeutic treatment of Tiypanosoma cnizi infection, to the use of these compounds, and to pharmaceutical compositions comprising them. Furthermore, this invention relates to compounds which are inhibitors across a broad range of cysteine proteases, to the use of these compounds, and to pharmaceutical compositions comprising them. Such compounds are useful for the therapeutic treatment of diseases in which participation of a cysteine protease is implicated.
The trypanosomal family of parasites have a substantial worldwide impact on human and animal healthcare (McKerrow, J. H., et al, Ann. Rev. Microbiol. 47, 821-853, 1993). One parasite of this family, Trypanosoma cruzi, is the causative agent of Chagas' disease, which affects in excess of twenty million people annually in Latin and South America, is the leading cause of heart disease in these regions and results in more than 45,000 deaths per annum (Centers for Disease Control and prevention website). In addition, with the increase in migration of the infected population from rural to urban sites and movements from South and Central America into North America, the infection is spreading via blood transfusions, and at birth. The present treatments of choice for Tiypanosoma cruzi infection, nifurtimox and benznidazole (an NADH fumarate reductase inhibitor, Turrens, JF, et al, Mol Biochem Parasitol, 82(1), 125-9, 1996) are at best moderately successful, achieving ~60% cure during the acute phase of infection (see Docampo, R. Curr. Pham. Design, 7, 1157-1164, 2001 for a general discussion) whilst not being prescribed at all during the chronic phase where cardiomyopathy associated heart failure often occurs (Kirchhoff, L. V. New Engl. J. Med., 329, 639-644,1993). Additionally, these two drugs have serious adverse toxic effects, requiring close medical, supervision during treatment, and have been shown to induce chromosomal damage in chagastic infants (Gorla, N. B. et al, Mutat. Res. 206, 217-220, 1988). Therefore, a strong medical need exists for new effective drugs for the chemo therapeutic treatment si Tiypanosoma cruzi infection.
Classically, the identification of enzymes found to be crucial for the establishment or propagation of an infectious disease has been instrumental in the development of successful drugs such as antivirals (e.g. HIV aspartyl protease inhibitors) and anti-bacterials (e.g. pMactam antibiotics). The search for a similar Achilles heel in parasitic infections has examined numerous enzymes (e.g. parasitic dihydrofolate reductase, see Chowdhury, S. F. et al, J. Med. Chem., 42(21), 4300-4312, 1999; trypanothione reductase, see Li, Z. et al, Bioorg. Med. Chem. Lett., 11 (2), 251-254, 2001; parasitic glyceraldehydes-3-phosphate dehydrogenase, see Aranov, A. M. et al, J. Med. Chem., 41(24), 4790-4799, 1998). A particularly promising area of research has identified the role of cysteine proteases, encoded by the parasite, that play a pivotal role during the life cycle of the parasite (McKerrow, J. H., et al, Bioorg. Med. Chem., 7, 639-644, 1999). Proteases form a substantial group of biological molecules winch to date constitute approximately 2% of all the gene products identified following analysis of several genome sequencing programmes (e.g. see Southan, C. J. Pept. Sci, 6, 453-458, 2000). Proteases have evolved to participate in an enormous range of biological processes, mediating their effect by cleavage of peptide amide bonds within the myriad of proteins found hi nature. This hydrolytic action is performed by initially recognising, then binding to, particular three-dimensional electronic surfaces displayed by a protein, which aligns the bond for cleavage precisely within the protease.catalytic site. Catalytic hydrolysis then commences through nucleophilic attack of the amide bond to be cleaved either via an amino acid side-chain of the protease itself, or through the action of a water molecule that is bound to and activated by the protease. Proteases in which the attacking nucleophile is the thiol side-chain of a Cys residue are known as cysteine proteases. The general classification of 'cysteine protease' contains many members found across a wide range of organisms from viruses, bacteria, protozoa, plants and fungi to mammals.
Biological investigation of Trypanosoma cruzi infection has highlighted a number of specific enzymes that are crucial for the progression of the parasite's life cycle. One such enzyme, cruzipain, a cathepsin L-like cysteine protease, is a clear therapeutic
target for the treatment of Chagas' disease ((a) Cazzulo, J. J. et al, Cwr. Pharm. Des. 7, 1143-1156, 2001; (b) Caffrey, C. R. etal, Cwr. Drug Targets, 1, 155-162, 2000). Although the precise biological role of cruzipain within the parasite's life cycle remains unclear, elevated cruzipain messenger RNA levels in the epimastigote developmental stage indicate a role in the nutritional degradation of host-molecules in lysosomal-like vesicles (Engel, J. C. et al, J. Cell. Sci, 111, 597-606,1998). The validation of cruzipain as a viable therapeutic target has been achieved with increasing levels of complexity. Addition of a general cysteine protease inhibitor, Z-Phe-Ala-FMK to Tiypanosoma cmzz-infected mammalian cell cultures blocked replication and differentiation of the parasite, thus arresting the parasite life cycle (Harth, G., et al, Mol Biochem. Parasitol 58, 17-24, 1993). Administration of a vinyl sulphone-based inhibitor in a Tiypanosoma crwzz-infected murine animal model not only rescued the mice from lethal infections, but also produced a complete recovery (Engel, J. C. et al, J. Exp. Med, 188(4), 725-734,1998). Numerous other in vivo studies have confirmed that infections with alternative parasites such as Leishmania major (Selzer, P. M. et al, Proc. Nat'l. Acad. Sci. U.S.A., 96, 11015-11022,1999), Schistosoma mansoni and Plasmodiumfalciparium (Olson, J. E. et al, Bioorg. Med. Chem., 7, 633-638, 1999) can be halted or cured by treatment with cysteine protease inhibitors.
A variety of synthetic approaches have been described towards the design of cruzipain inhibitors. However, although providing a biological 'proof-of-principle' for the treatment of Trypanosoma cruzi infection, current inhibitors exhibit a number of biochemical and physical properties that may preclude their clinical development (e.g. see (a) Brinen, L. S. et al, Structure, 8, 831-840, 2000, peptidomimetic vinyl sulphones, possible adverse mammalian cell toxicity (see McKerrow, J. H. and Engel, J. unpublished results cited in Scheldt, K. A. et al, Bioorg. Med. Chem, 6, 2477-2494, 1998); (b) Du, X. et al, Chem. Biol, 7, 733-742, 2000, aryl ureas, generally with low uM activity, and high ClogP values, thus poor aqueous solubility and probably low oral bioavailability; (c) Roush, W. R. et al, Tetrahedron, 56, 9747-9762, 2000, peptidyl epoxysuccinates, irreversible inhibitors, with potent activity verses house-keeping mammalian proteases such as cathepsin B; (d) Li, R. et al,
Bioorg. Med. Chem. 4(9), 1421-1427, 1996, bisarylacylhydrazides and chalcones, polyhydroxylated aromatics; (e) US6143931, WO 9846559, non-peptide a-ketoamides). Of the many different approaches to enzyme inhibition to date, only the cruzipain protease inhibitors have proven effective in curing disease-related animal models of Trypanosoma cruzi infection. Therefore, a clear medical need exists to progress these 'proof-of-principle' findings towards clinical candidates, suitable for human use, through the discovery of more efficacious cruzipain inhibitors that have a desirable combination of potency, selectivity, low toxicity and optimised pharmacokinetic parameters.
Cruzipain and indeed many other crucial parasitic proteases belong to the papain-like CA Cl family and have close structural mammalian homologues. Cysteine proteases are classified into 'clans' based upon a similarity in the three-dimensional structure or a conserved arrangement of catalytic residues within the protease primary sequence. Additionally, 'clans' are further classified into 'families' in which each protease shares a statistically significant relationship with other members when comparing the portions of amino acid sequence which constitute the parts responsible for the protease activity (see Barrett, AJ et al, in 'Handbook of Proteolytic Enzymes', Eds. Barrett, A. J., Rawlings, N. D., and Woessner, J. F. Publ. Academic Press, 1998, for a thorough discussion). To date, cysteine proteases have been classified into five clans, CA, CB, CC, CD and CE (Barrett, A. J. et al, 1998). A protease from the tropical papaya fruit 'papain' forms the foundation of clan CA, which currently contains over 80 distinct and complete entries in various sequence databases, with many more expected from the current genome sequencing efforts. Proteases of clan CA/family Cl have been implicated in a multitude of disease processes e.g. human proteases such as cathepsin K (osteoporosis), cathepsin S (autoimmune disorders), cathepsin L (metastases) or parasitic proteases such as falcipain (malaria parasite Plasmodium falciparum), cruzipain (Trypanosoma cruzi infection). Recently a bacterial protease, staphylopain (S. aureus infection) has also been tentatively assigned to clan CA. X-ray crystallographic structures are available for a range of the above mentioned proteases in complex with a range of inhibitors e.g. papain (PDB entries, Ipad, Ipe6, Ipip, Ipop, 4pad, 5pad, 6pad, Ippp, Ithe, Icsb,
Ihuc), cathepsin K (lauO, Iau2, Iau3, Iau4, latk, Imem, Ibgo, layw, layu), cathepsin L (Ics8), cathepsin S (currently on-hold, but published McGrath, M. E. et al, Protein Science, 7, 1294-1302,1998), cruzain (a recombinant form of cruzipain see Eakin, A. E. et al, 268(9), 6115-6118, 1993) (lewp, laim, 2aim, 1F29, 1F2A, 1F2B, 1F2C), staphylopain (Icv8). Each of the structures displays a similar overall active-site topology, as would be expected by their 'clan' and 'family' classification and such structural similarity exemplifies one aspect of the difficulties involved in discovering a selective inhibitor of cruzipain suitable for human use. However, subtle differences in terms of the depth and intricate shape of the active site groove of each CA Cl protease are evident, which may be exploited for selective inhibitor design. Additionally, many of the current substrate-based inhibitor complexes of CA Cl family proteases show a series of conserved hydrogen bonds between the inhibitor and the protease backbone, which contribute significantly to inhibitor potency. Primarily a bidentate hydrogen-bond is observed between the protease Gly66 (C=O)/ inhibitor N-H and the protease Gly66(NH)/inhibitor (C=O), where the inhibitor (C=O) and (NH) are provided by an amino acid residue NHCHRCO that constitutes the S2 sub-site binding element within the inhibitor (see Berger, A. and Schecter, I. Philos. Trans. R. Soc. Lond. [Biol.J, 257.249-264,1970 for a description of protease binding site nomenclature). A further hydrogen-bond between the protease main-chain (C=O) of asparagine or aspartic acid (158 to 163, residue number varies between proteases) and an inhibitor (N-H) is often observed, where the inhibitor (N-H) is provided by the SI sub-site binding element within the inhibitor. Thus, the motif X-NHCHRCO-NH-Y is widely observed amongst the prior art substrate-based inhibitors of CA Cl proteases.
In the prior art, the development of cysteine protease inhibitors for human use has recently been an area of intense activity. Considering the CA Cl family members, particular emphasis has been placed upon the development of inhibitors of human cathepsins, primarily cathepsin K (osteoporosis), cathepsin S (autoimmune disorders) and cathepsin L (metastases), through the use of peptide and peptidomimetic nitriles (e.g. see WO-A-0109910, WO-A-0051998, WO-A-0119816, WO-A-9924460, WO-A-0049008, WO-A-0048992, WO-A-0049007, WO-A-0130772, WO-A-0055125,
WO-A-0055126, WO-A-0119808, WO-A-0149288, WO-A-0147886), linear and cyclic peptide and peptidomimetic ketones (e.g. see Veber, D. F. and Thompson, S. K., Cuir. Opin. Diiig Discovery Dev., 3(4), 362-369,2000, WO-A-0170232, WO-A-0178734, WO-A-0009653, WO-A-0069855, WO-A-0029408, WO-A-0134153 to WO-A-0134160, WO-A-0029408, WO-A-9964399, WO-A-9805336, WO-A-9850533), ketoheterocycles (e.g. see WO-A-0055144, WO-A-0055124) and monobactams (e.g. see WO-A-0059881, WO-A-9948911, WO-A-0109169). The prior art describes potent in vitro inhibitors, but also highlights the many difficulties in developing a human therapeutic. For example, WO-A-9850533 and WO-A-0029408 describe compounds that may be referred to as cyclic ketones and are inhibitors of cysteine proteases with a particular reference towards papain family proteases and as a most preferred embodiment, cathepsin K. WO-A-9850533 describes compounds subsequently detailed in the literature as potent inhibitors of cathepsin K with good oral bioavailability (Witherington, J., 'Tetrahydrofurans as Selective Cathepsin K Inhibitors', RSC meeting, Burlington House, London, 1999). The compounds of WO-A-9850533 were reported to bind to cathepsin K through the formation of a reversible covalent bond between the tetrahydrofuran carbonyl and the active site catalytic cysteine residue (Witherington, J., 1999). Additionally, the same cyclic ketone compounds are described in WO-A-9953039 as part of a wide-ranging description of inhibitors of cysteine proteases associated with parasitic diseases, with particular reference to the treatment of malaria by inhibition of falcipain. However, subsequent literature describes the cyclic ketone compounds of WO-A-9850533 to be unsuitable for further development or for full pharmacokinetic evaluation due to a physiochemical property of the inhibitors, the poor chiral stability of the ct-aminoketone chiral centre (Marquis, R. W. et al, J. Med. Chem., 44(5), 725-736, 2001). WO-A-0069855 describes compounds that may also be referred to as cyclic ketones with particular reference towards inhibition of cathepsin S. The compounds of WO-A-0069855 are considered to be an advance on compounds of WO-A-9850533 due to the presence of the p-substituent on the cyclic ketone ring system that provides chiral stability to the ct-carbon of the cyclic ketone ring system. However, the compounds of WO-A-0069855 and indeed those of WO-A-9850533 describe a requirement for the presence of the potential hydrogen-bonding motif X-
KHCHRCQ-NH-Y that is widely observed amongst the prior art substrate-based inhibitors of CA Cl proteases.
It has now been discovered that certain compounds, defined by general formulae (I)
and (II), are potent and selective cruzipain protease inhibitors which are useful in the
treatment of Trypanosoma cmzi infection. Other compounds defined by general
formulae (I) and (II) are protease inhibitors across a broad range of CA Cl cysteine
proteases and compounds useful in the treatment of diseases caused by cysteine
proteases. Compounds described by general formulae (I) and (II) do not contain the
X-NHCHRCO-NH-Y motif that is widely observed amongst the prior art substrate-
based inhibitors of CA Cl proteases, yet surprisingly compounds defined by general
formulae (I) and (II) retain good potency. The present invention provides substituted
tetrahydrofuro[3 ,2-6]pyrrol-3-one, tetrahydrothieno[3,2-Z>]pyrrol-3-one,
hexahydropyrrolo[3 ,2-&]pyrrol-3-one, hexahydrocyclopenta[6]pyrrol-6-one,
tetrahydrofuro[3,2-c]pyrazol-6-one, tetrahydrothieno[3,2-c]pyrazol-6-one,
hexahydropyrrolo[3,2-c]pyrazol-6-ones hexahydrocyclopentapyrazol-6-one,
hexahydrofuro[3,2-fc]pyridin-3-one, hexahydrothieno[3,2-fc]pyridin-3-one,
octahydropyrrolo[3,2-fc]pyridin-3-one, octahydrocyclopenta[fc]pyridin-7-one,
hexahydrofuro[3 ,2-c]pyridazin-7-one, hexahydrotbieno[3 ,2-c]pyridazin-7-one,
octahydropyrrolo[3,2-c3pyridazin-7-one or octahydrocyclopenta[c]pyridazin-7-one bicyclic compounds defined by general formulae (I) and (II).
Accordingly, the first aspect of the invention provides a compound according to general formula (I) or general formula (II): -
wherein: R1 = C0-7-alkyl (when C = 0, R1 is simply hydrogen), Cs-e-cycloalkyl or Ar-Co-7-alkyl (when C = 0, R1 is simply an aromatic moiety Ar);
Z = O, S, CR2R3 or NR4, where R4 is chosen from C0.ralkyl, C3.6-cycloalkyl or Ar-Co-7-alkyl;
P1 = CR5R6, P2 = CR7R8,
Q = CR9R10 orNR11, where Rn is chosen from C0-7~alkyl, C3.6-cycloalkyl or Ar-Co-7-alkyl;
Each of R2, R3, R5, R6, R7, R8, R9 and R10 is independently chosen from Co-7-alkyl, C3^-cycloalkyl, Ar-Co-7-alkyl, O-C0-7-alkyl, O-C3^-cycloalkyl, O-Ar-CVralkyl, S-C0.7-alkyl, S-C3.6-cycloalkyl, S-Ar-Co-7-alkyl, NH-Q,. 7-alkyl, NH-C3^-cycloalkyl, NH-Ar-Co-7-alkyl, N(Co-7-alkyl)2, N(C3.6-cycloalkyl)2 orN(Ar-Co-7-alkyl)2;
Y = CR12R13-CO, where R12, R13 are chosen from Co-7-alkyl, C3.6-cycloalkyl or Ar-Co-7-alkyl;
(X)0 = CR14R15, where R14 and R15 are independently chosen from CQ.?-alkyl, Cs-e-cycloalkyl and Ar-Co-7-alkyl and o is a number from zero to three;
(W)n = O, S, C(O), S(O) or S(O)2 or NR16, where R16 is chosen from Co-7-alkyl, C3.6-cycloalkyl and Ar-Co-7-alkyl and n is zero or one;
(V)m = C(0), C(S), S(0), S(0)2, S(0)2NH, OC(O), NHC(O), NHS(O), NHS(0)2, OC(O)NH, C(0)NH or CR17R18, where R17 and R18 are independently chosen from Co-7-alkyl, C3.6-cycloalkyl, Ar-Co-y-alkyl and m is a number from zero to three, provided that when m is greater than one, (V)m contains a maximum of one carbonyl or sulphonyl group;
U = a stable 5- to 7-membered monocyclic or a stable 8- to 11-membered bicyclic ring which is either saturated or unsaturated and which includes zero to four heteroatoms (as detailed below):

wherein R19 is:
Co-7-alkyl, C3^-cycloalkyl, Ar-Co-7-alkyl, O-Co-7-alkyl, O-C3.e-cycloalkyl, O-Ar-C0-7-alkyl, S-C0-7-alkyl, S-C3.6-cycloalkyl, S-Ar-C0-7-alkyl, NH-Co.7-alkyl, NH-C3.6-cycloalkyl, NH-Ar-C0-7-alkyl, N(C0.7-alkyl)2, N(C3.6-cycloalkyl)2 or N(Ar-Co-7-alkyl)2; or, when part of a CHR19 or CR19 group, R19 may be halogen; A is chosen from:
CH2,CHR19sO,SandNR20;
where R19 is as defined above; and R20 is chosen from:
Co-7-alkyl, C3.6-cycloalkyl and Ar-C0-7-alkyl;
B, D and G are independently chosen from:
CR19, where R19 is as defined above, or N;
E is chosen from:
CH2, CHR19, O, S and NR20, where R19 and R20 are defined as above;
J, L, M, R, T, T2, Ts and T4 are independently chosen from: CR19 and N, where R19 is as defined above;
TS is chosen from: CHorN;
q is a number from one to three, thereby defining a 5-, 6- or 7-membered ring.
B, D, G, J, L, M, R, T, T2, T3 and T4 may additionally represent an N-oxide (N->O).
The present invention includes all salts, hydrates, solvates, complexes and prodrugs of the compounds of this invention. The term "compound" is intended to include all such salts, hydrates, solvates, complexes and prodrugs, unless the context requires otherwise.
Appropriate pharmaceutically and veterinarily acceptable salts of the compounds of general formulae (I) and (II) include salts of organic acids, especially carboxylic acids, including but not limited to acetate, trifluoroacetate, lactate, gluconate, citrate, tartrate, maleate, malate, pantothenate, adipate, alginate, aspartate, benzoate, butyrate, digluconate, cyclopentanate, glucoheptanate, glycerophosphate, oxalate, heptanoate, hexanoate, fumarate, nicotinate, pahnoate, pectinate, 3-phenylpropionate, picrate, pivalate, proprionate, tartrate, lactobionate, pivolate, camphorate, undecanoate and succinate, organic sulphonic acids such as methanesulphonate,
ethanesulphonate, 2-hydroxyethane sulphonate, camphorsulphonate, 2-naphthalenesulphonate, benzenesulphonate, p-chlorobenzenesulphonate and p-toluenesulphonate; and inorganic acids such as hydrochloride, hydrobromide, hydroiodide, sulphate, bisulphate, hemisulphate, thiocyanate, persulphate, phosphoric and sulphonic acids. Salts which are not pharmaceutically or veterinarily acceptable may still be valuable as intermediates.
Prodrugs are any covalently bonded compounds which release the active parent drug
according to general formulae (I) or (II) in vivo. A prodrug may for example
constitute an acetal or hemiacetal derivative of the exocyclic ketone functionality
present in the tetrahydrofuro[3,2-6]pyrrol-3-one, tetrahydrothieno[3,2-6]pyrrol-3-
one, hexahydropyrrolo[3,2-6]pyrrol-3-one, hexahydrocyclopenta[&]pyrrol-6-one,
tetrahydrofuro[3,2-c]pyrazol-6-one, tetrahydrothieno[3,2-c]pyrazol-6-one,
hexahydropyrrolo[3,2-c]pyrazol-6-one, hexahydrocyclopentapyrazol-6-one,
hexahydrofuroES^-^pyridin-S-one, hexahydrothieno[3,2-6]pyridin-3-one,
octahydropyrrolo[3,2-ft]pyridin-3-one, octahydrocyclopenta[6]pyridin-7-one,
hexahydrofuro[3,2~c]pyridazin-7-one, hexahydrothieno[3,2-c]pyridazin-7-one,
octahydropyrrolo[3,2-c]pyridazin-7-one or octahydrocyclopenta[c]pyridazin-7-one scaffold. If a chiral centre or another form of isomeric centre is present in a compound of the present invention, all forms of such isomer or isomers, including enantiomers and diastereoisomers, are intended to be covered herein. Compounds of the invention containing a chiral centre may be used as a racemic mixture, an enantiomerically enriched mixture, or the racemic mixture may be separated using well-known techniques and an individual enantiomer may be used alone.
'fjalogen' as applied herein is meant to include F, Cl, Br, I; 'Heteroatom' as applied herein is meant to include O, S and N;
'Co-7-alkyl' as applied herein is meant to include stable straight and branched chain aliphatic carbon chains containing zero (i.e. simply hydrogen) to seven carbon atoms such as methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, ,t-butyl, pentyl,
isopentyl, hexyl, heptyl and any simple isomers thereof. Additionally, any Co-v-alkyl may optionally be substituted at any point by one, two or three halogen atoms (as defined above) for example to give a trifhioromethyl substituent. Furthermore, CQ.?-alkyl may contain one or more heteroatoms (as defined above) for example to give ethers, thioethers, sulphones, sulphonamides, substituted amines, amidines, guanidines, carboxylic acids, carboxamides. If the heteroatom is located at a chain terminus then it is appropriately substituted with one or two hydrogen atoms. A heteroatom or halogen is only present when Co-v-alkyl contains a minimum of one carbon atom.
'Cs-e-cycloalkyF as applied herein is meant to include any variation of 'Co-7-alkyl' which additionally contains a carbocyclic ring such as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl. The carbocyclic ring may optionally be substituted with one or more halogens (as defined above) or heteroatoms (as defined above) for example to give a tetrahydrofuran, pyrrolidine, piperidine, piperazine or morpholine substituent.
' Ar-Co-7-alkyl' as applied herein is meant to include any variation of Co-7-alkyl which additionally contains an aromatic ring moiety 'Ar'. The aromatic ring moiety Ar can be a stable 5 or 6-membered monocyclic or a stable 9 or 10 membered bicyclic ring which is unsaturated, as defined previously for U in general formulae (I) and (II). The aromatic ring moiety Ar may be substituted by R19 (as defined above for U in general formulae (T) and (II)). When C = 0 in the substituent Ar-Co-7-alkyl, the substituent is simply the aromatic ring moiety Ar.
Other expressions containing terms such as alkyl and cycloalkyl are intended to be construed according to the definitions above. For example "CM alkyl" is the same as Co-7-alkyl except that it contains from one to four carbon atoms.
If different structural isomers are present, and/or one or more chiral centres are present, all isomeric forms are intended to be covered. Enantiomers are characterised by the absolute configuration of their chiral centres and described by the R- and S-
sequencing rules of Cahn, Ingold and Prelog. Such conventions are well known in the art (e.g. see 'Advanced Organic Chemistry', 3rd edition, ed. March, J., John Wiley and Sons, New York, 1985).
Preferred compounds of general formulae (I) and (II) include, but are not limited to, those in which, independently or in combination:
ZisO,S,NHorCH2;
P1andP2areCH2;and
QisCH2orNH.
Also, preferred compounds of general formulae (I) and (II) include, but are not limited to, those in which, independently or in combination
Z is MR4, where R4 is a Ar-Ci-4-alkyl or a substituted carbonyl or sulphonyl group;
P1 andP2areCH2;and
QisCH2orNH.
Thus, examples of preferred compounds include those containing a bicyclic moiety
chosen from tetrahydrofuro[3,2-6]pyrrol-3-one, tetrahydrothieno[3,2-6]pyrrol-3-one,
hexahydropyrrolo[3,2-6]pyrrol-3-oue, hexahydrocyclopenta[6]pyrrol-6-one,
tetrahydrofuro[3,2-c]pyrazol-6-one, tetrahydro-thieno[3,2-c]pyrazol-6-one,
hexahydropyrrolo[3,2-c]pyrazol-6-one, hexahydro-cyclo-pentapyrazol-6-one,
hexahydrofuro[3,2-6]pyridin-3-one, hexahydrothieno[3,2-6]pyridin-3-one,
octahydropyrrolo[3,2-6]pyridin-3-one, octahydrocyclopenta[i]pyridin-7-one,
hexahydrofuro[3,2-c]pyridazin-7-one, hexahydrothieno[3,2-c]pyridazin-7-one,
octahydropyrrolo[3,2-c]pyridazin-7-one or octahydrocyclopenta[c]pyridazin-7-one as shown below.
In compounds of general formulae (I) and (II), it is preferred that R1 comprises CQ.?-alkyl or Ar-Co-7-alkyl. Thus, for example, preferred R1 moieties include hydrogen, or a straight or branched alkyl chain, or a straight or branched heteroalkyl chain, or an optionally substituted arylalkyl chain, or an optionally substituted arylheteroalkyl chain.
It is particularly preferred that R1 is hydrogen or Q-4 alkyl or Ar-Ci-4-alkyl and examples of such R1 substituents include, but are not limited to:
where R19 is defined above.
In preferred compounds of general formulae (I) and (II), Y is CHR13CO where R13 is selected from Co-7-alkyl or Ar-Co.7-alkyl, for example hydrogen, a straight or branched alkyl chain, a straight or branched heteroalkyl chain, an optionally substituted arylalkyl chain or an optionally substituted arylheteroalkyl chain. Additionally, in preferred compounds of general formulae (I) and (II), R13 is selected from Ca-e-cycloalkyl, for example cyclohexylmethyl.
Examples of preferred Y substituents include the following:

wherein R1*, R2U and Ar are as defined above.
More preferred R13 groups include Ci-4-alkyl, which may be substituted with OH, NR20R20, COOR20, or CONR20 or cycloalkylmethyl or Ar-CM-alkyl, where the aryl group may be substituted with R19; wherein each R19 and R20 is independently as defined above.
Even more preferred R13 groups comprise Ar-CHb-, where the aromatic ring is an optionally substituted phenyl or monocyclic heterocycle. Additionally, even more preferred R13 groups comprise simple branched alkyl groups such as isobutyl or straight heteroaUcyl chains such as benzylsulfanylmethyl or benzylsulphonylmethyl. Furthermore, even more preferred R13 groups comprise cyclohexylmethyl. Examples of even more preferred Y substituents comprise the following,

.19
wherein R and Ar are as defined previously

It is preferred that in the group (X)0, each of R14 and R15 is selected from C0-7-alkyl or Ar-Co-7-alkyl, for example hydrogen, a straight or branched alkyl chain, a straight or branched heteroalkyl chain, an optionally substituted arylalkyl chain or an optionally substituted arylheteroalkyl chain.
More preferred (X)0 groups comprise R14 chosen from hydrogen; R15 is Ci_4-alkyl, which may be substituted with OH, NR20R20, COOR20, or CONR20; or Ar-CM-alkyl, where the aryl group may be substituted with R19, wherein each R19 and R20 is independently as defined above.
wherein R and R are as efined previously.
Even more preferred ompounds of geeral formulae (I) and (II), comprise (X)0 groups that are simple alkyl groups such as methylene and where o = 0 or 1.
In the group (W)n, W is preferably O, S, SO2, S(O), C(O) or NR16, where R16 is Chalky!; and n is 0 or 1.
More preferred compounds of general formulae (I) and (II), comprise (W)n groups defined as O, S, SO2, C(O) and NH where n = 0 or 1.
Yet even more preferred compounds of general formulae (I) and (II), comprise (W)n groups defined as NH where n = 1.
In the group (V)m:
V is preferably C(O), C(O)NH or CHR18, where R18 is Qn-alkyl; and m is 0 or 1.
Preferred V and W substituent combinations encompassed by general formulae (I) and (II) include, but are not limited to:

Additionally, a preferred V and W substituent combination encompassed by general formulae (I) and (II) includes, but is not limited to:
More preferred V, W and X substituent combinations encompassed by general formulae (I) and (II) comprise, but are not limited to

In preferred compounds of general formulae (I) and (II), U comprises an optionally substituted 5- or 6-membered saturated or unsaturated heterocycle or Ar group or an optionally substituted saturated or unsaturated 9- or 10-membered heterocycle or Ar group. Examples of such preferred U rings include the following:
More preferred compounds of general formulae (I) and (II), contain a U group comprising of a bulky alkyl or aryl group at the para position of an aryl Ar. Also, more preferred compounds contain a meta or para-biaryl Ar-Ar, where Ar is as previously defined. Additionally, more preferred compounds contain a 6,6 or 6,5 or 5,6-fused aromatic ring. Examples of more preferred U groups are
wherein R19, D, E, G, J, L, M, R, T, T2, T3 and T4 are as defined previously.
Even more preferred compounds of general formulae (I) and (II), particularly for inhibition of cruzipain, contain a U group comprising a 6-membered Ar ring containing a bulky alkyl or aryl group at the para position, where Ar is as previously defined

formulae (I) and (II), contain a U group comprising but are not limited to the following,

wherein R19, D, E, G, J and L are as defined previously.
Abbreviations and symbols commonly used in the peptide and chemical arts are used herein to describe compounds of the present invention, following the general

guidelines presented by the TUP AC-TUB Joint Commission on Biochemical Nomenclature as described in Eur. J. Biochem., 158, 9-, 1984. Compounds of formulae (I) and (II) and the intermediates and starting materials used in then-preparation are named in accordance with the IUPAC rules of nomenclature in which the characteristic groups have decreasing priority for citation as the principle group. An example compound of formula (I), compound (1) in which R1 is H, PI is methylene, Q is methylene, Z is oxygen, Y is 4-methylpentyl, (X)o is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:-
(3aS,6a£)4-(2S-Berizyloxy-4-methylpentano
A second example compound of formula (I), compound (2) in which R1 is H, Pi is methylene, Q is methylene, Z is sulphur, Y is 4-methylpentyl, (X)o is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:-
(2) (3aR,6aR) 4-(25-Benzyloxy-4-methylpentanoyl)tetrahydrothieno[3,2-6]pyrrol-3-one
A third example compound of formula (I), compound (3) in R1 is H, PI is methylene, Q is methylene, Z is methylene, Y is 4-methylpentyl, (X)o is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:-
l-(2S-Benzyloxy-4-methylpentanoyl)hexahydrocyclopenta[6]pyrrol-6-one
A fourth example compound of formula (I), compound (4) in R1 is H, PI is methylene, Q is methylene, Z is NH, Y is 4-methylpentyl, (X)o is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:-
(3 aS,6aR) 1 -(25-Ben2yloxy-4-methylpentauoyl)hexahydropyrrolo[3,2-6]pyrrol-3-one
A fifth example compound of formula (I), compound (5) in which R1 is H, PI is methylene, Q is NH, Z is oxygen, Y is 4-methylpentyl, (X)0 is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:-

(3a7?,6a5)4-(25'-Benzyloxy-4-methylpentanoyl)tetrahydrofuro[352-c]pyrazol-6-one
A sixth example compound of formula (I), compound (6) in which R1 is H, PI is methylene, Q is NH, Z is sulphur, Y is 4-methylpentyl, (X)o is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:-
(3a/?,6a/?)4-(25'-Benzyloxy-4-methylpentanoyl)tetrahydrothieno[3,2-c]pyrazol-6-one
A seventh example compound of formula (I), compound (7) in R1 is H, PI is methylene, Q is NH, Z is methylene, Y is 4-methylpentyl, (X)o is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:-
-(25-Benzyloxy-4-methylpentanoyl)hexahydrocyclopentapyrazol-6-one
An eighth example compound of formula (I), compound (8) in R1 is H, PI is methylene, Q is NH, Z is NH, Y is 4-methylpentyl, (X)o is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:-
(3a/?,6aS) l-(2iSr-Ben2yloxy-4-methylpentanoyl)hexahydropyrrolo[3,2-c]pyrazol-6-one
An example compound of formula (II), compound (9) in which R1 is H, PI is methylene, ?2 is methylene, Q is methylene, Z is oxygen, Y is 4-methylpentyl, (X)o is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:-
(3a5,7aR)4-(2iSr-Ben2yloxy-4-methylpentanoyl)hexahydrofuro[3,2-fe]pyridin-3-one
A second example compound of formula (II), compound (10) in which R1 is H, PI is methylene, P2 is methylene, Q is methylene, Z is sulphur, Y is 4-methylpentyl, (X)o is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:-
(3a7?,7a/?)4-(2*S-Benzyloxy-4-methylpentanoyl)hexahydrotiiieno[3,2-fe]pyridin-3-one
A third example compound of formula (II), compound (11) in which R1 is H, P! is methylene, P2 is methylene, Q is methylene, Z is methylene, Y is 4-methylpentyl, (X)0 is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:-

(4aS,7aS) l-(25-Benzyloxy-4-methylpentanoyl)octahydrocyclopenta[6]pyridin-7-one
A fourth example compound of formula (II), compound (12) in which R1 is H, PI is methylene, Pa is methylene, Q is methylene, Z is NH, Y is 4-methylpentyl, (X)0 is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:-
(3a5,7a^)4-(21S-Ben2yloxy-4-methylpentanoyl)octahydropyrrolo[3,2-i]pyridin-3-one
A fifth example compound of formula (II), compound (13) in which R1 is H, PI is methylene, P2 is methylene, Q is NH, Z is oxygen, Y is 4-methylpentyl, (X)o is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:-

(4a/?,7a5)4-(25-Benzyloxy-4-methylpentanoyl)hexahydrofuro[3,2-c]pyridazin-7-one
A sixth example compound of formula (II), compound (14) in which R1 is H, PI is methylene, P2 is methylene, Q is NH, Z is sulphur, Y is 4-methylpentyl, (X)o is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:-

(4a^,7a/?)4-(21S-Benzyloxy-4-methylpentanoyl)hexahydrofuro[3,2-c]pyridazin-7-
one
A seventh example compound of formula (II), compound (15) in which R1 is H, PI is methylene, P2 is methylene, Q is NH, Z is methylene, Y is 4-methylpentyl, (X)o is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:-

-(25-Ben2yloxy-4-methylpentanoyl)octahydrocyclopenta[c]pyridazin-7-
one
An eighth example compound of formula (II), compound (16) in which R1 is H, PI is methylene, Pa is methylene, Q is NH, Z is NH, Y is 4-methylpentyl, (X)0 is zero, (W)n is oxygen, (V)m is methylene and U is phenyl is thus named:-
4-(21Sf-Benzyloxy-4-methylpentanoyl)octahydropyrrolo[3,2-c]pyridazhi-7-
one
Compounds of the invention include, but are not limited to, the following examples that are the (3aS, 6aR) isomer of general formula (I), where Z = 'O' and R1 = 'H', and also include the equivalent analogues included in the full definition of Z and R1
7V-[l-(4-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydroiuro[3,2-6]pyrrol-4-yl)ethyl] benzamide
4-/ert-Butyl-JV-[l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofiiro[3,2-Z)]pyrrol-4-yl)ethyl]benzamide
Ar-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]-4-trifluoromethoxyb enzamide
4-Dimethylamino-7V'-[l-(4-liydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[332-6]pyrrol-4-yl)ethyl]benzamide
7Vr-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydroftiro[3,2-i]pyrrol-4-yl)ethyl]-4-isopropylbenzamide
4-Difluoromethoxy-7V-[l-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fc]pyrrol-4-yl)etiiyl]benzamide
7V-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyirol-4-yl)ethyl]-4-trifluoromethylbenzamide
4-Bromo-JV-[l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide
3-Bromo-JV-[l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-liexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide
jV-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-^]pyrrol-4-yl)ethyl]-4-vinylbenzamide
Naphthalene-2-carboxylic acid [l-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-fe]pyrrol-4-yl)ethyl]amide
Naphthalene-1-carboxylic acid [l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3 52-6]pyrrol-4-yl)ethyl]amide
Quinoline-6-carboxylic acid [1 -(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-fc]pyrrol-4-yl)ethyl]amide
Benzo[b]thiophene-2-carboxylic acid [1 -(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-&]pyrrol-4-yl)ethyl]amide
Benzo[6]thiophene-3-carboxylic acid [l-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-fe]pyrrol-4-yl)ethyl]amide
Benzothiazole-5-carboxylic acid [l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-b.exahydro furo[3,2-6]pyrrol-4-yl)ethyl]amide
Biphenyl-4-carboxylic acid [ 1 -(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]amide
/A[l-(4-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-phenoxybenzamide
AT-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-fe]pyrrol-4-yl)ethyl]-3-phenoxybenzamide
N-[l -(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]-4-imidazol-1 -ylbenzamide
//-[l-(4-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-^]pyrrol-4-yl)ethyl]-4-thiopb.en-2-ylbenzamide
JV-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydroftiro[3,2-&]pyrrol-4-yl)ethyl]-4-oxazol-5 -ylbenzamide
jV"-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-[1,2,3]thiadiazol-5-ylbenzamide
.V-[l.(4.Hydroxybeiizyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-pyrazol-1 -ylbenzamide
N-[l-(4-Hydroxybeiizyl)-2-oxo-2-(3-oxo-liexahydrofuro[3,2-fe]pyrrol-4-yl)ethyl]-5-tMopb.en-2-ylnicotmainide
Ar-[l 2-Phenylthiazole-4-carboxylic acid [l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa b.ydrofuro[3,2-6]pyrrol-4-yl)ethyl]ainide
2-Pyridin-3-ylthiazole-4-carboxylic acid [l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
5-Pb.enyltMophaie-2-carboxylic acid [1 -(4-hydroxybenzyl)-2-oxo-2-(3-oxo-liexa hydrofuro[3,2-fe]pyrrol-4-yl)ethyl]amide
5-Pyridin-3-ylthiophene-2-carboxylic acid [l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
2-Methyl-5-phenylfuran-3-carboxylic acid [1 -(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexab.ydrofuro[352-fc]pyrrol-4-yl)ethyl]amide
4-Phenylthiophene-2-carboxylic acid [1 -(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa b.ydrofuro[3,2-6]pyrrol-4-yl)etiiyl]amide
4-Pyridin-3-yltbiophene-2-carboxylic acid [l-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fc]pyrrol-4-yl)ethyl]amide
2-Tbiophen-2-ylthiazole-4-carboxylic acid [1 -(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]amide
2-Methyl-5-(pyrrolidine-l-sulfonyl)furan-3-carboxylic acid [l-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
3-Phenylpyrrole-l-carboxylic acid [l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-^]pyrrol-4-yl)ethyl]amide
^-[3-Meth.yl-l-(3-oxo-hexahydrofuro[3,2-t]pyrrole-4-carbonyl)butyl]beo2ainide
benzamide
JV-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-fe]pyrrole-4-caibonyl) butyl]-4-trifluoro methoxybenzamide
4-Dime%lamino-JV'-[3-meihyl-l-(3-oxo-hexahydrofuro[3,2-fe]pyrrole-4-carbonyl) butyl]benzamide
4-Isopropyl-^-[3-methyl-l-(3-oxo-hexahydrofuro[3,2-i]pyrrole-4-carbonyl) butyl] benzamide
4-Difluoromethoxy-7V'-[3-methyl-l-(3-oxo-hexab.ydrofuro[3,2-fe]pyrrole-4-carbonyl) butyl]benzamide
^T-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-&]pyrrole-4-cafbonyl) butyl]-4-trifluoro methylbenzamide
4-Bromo-^-[3-methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl] benzamide
3-Bromo-N-[3-methyl-1 -(3-oxo-hexahydn3furo[3,2-6]pyrrole-4-carbonyl) butyl] benzamide
^-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-Zj]pyrrole-4-carbonyl) butyl]-4-vinyl
benzamide
Naphthalene-2-carboxylic acid [3 -methyl-1 -(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl] amide
Naphthalene-1-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-Z)]pyrrole-4-carbonyl) butyl]amide
Quinoline-6-cafboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]amide
Benzo[&]thiophene-2-carboxylic acid [3-methyl-l -(3-oxo-hexahydrofuro[3,2-&] pyrrole-4-carbonyl) butyl]amide
Benzo[&]thiophene-3-carboxylic acid [3-methyl-l -(3-oxo-hexahydrofuro[3,2-&] pyrrole-4-carbonyl) butyl]amide
Benzothiazole-5-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl] amide
Biphenyl-4-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) buryl]amide
7^-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]-4-phenoxy benzamide
7V-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]-3-phenoxy benzamide
4-Imidazol-l-yl-AT-[3-methyl-l-(3-oxo-liexahydrofuro[3}2-t]pyrrole-4-carbonyl) butyljbenzamide
Ar-[3-Methyl-l-(3-oxo-hexahydrofiiro[352-6]pyrrole-4-carbonyl) butylj-4-thiophen-2-ylbenzamide
JV-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]-4-oxazol-5-ylbenzamide
]V-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]-4-[ 1,2,3] thiadiazol-4-ylbenzamide
7V-[3-Methyl-l-(3-oxo-liexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]-4-pyrazol-l-ylbenzamide
7sT-[3-Methyl-l-(3-oxo-hexaliydrofuro[3}2-6]pyrrole-4-carbonyl) butyl]-5-tbiophen-2-ylnicotinamide
JV-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyTrole-4-carbonyl) butylj-6-phenyl nicotinamide
2-Phenylthiazole-4-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-6] pyrrole-4-carbonyl) butyl]amide
2-Pyridin-3-ylthiazole-4-carboxylicacid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-Z?]pyrrole-4-cafbonyl) butyl]amide
5-Phenylthiophene-2-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-&] pyrrole-4-carbonyl) butyl]amide
5-Pyridm-3-ylthiophene-2-carboxylic acid [3-metiiyl-l -(3-oxo-hexahydrofuro[3,2-6] pyrrole-4-carbonyl) butyl]amide
2-Methyl-5-phenylfuran-3-carboxylic acid [3-memyl-l-(3-oxo-hexahydrofuro[3,2-6] pyrrole-4-carbonyl) butyl]amide
4-Phenylthiophene-2-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-6] pyrrole-4-carbonyl) butyl] amide
4-Pyridin-3-ylthiophene-2-carboxylic acid [3-methyl-1 -(3-oxo-hexahydrofuro [3,2-b] pyrrole-4-carbonyl) butyl]amide
2-Thiopb.en-2-ylthiazole-4-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3s2-^] pyrrole-4-carbonyl) butyljamide
2-Methyl-5-(pyn-oUdine-l-sulfonyl)furan-3-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-6] pyrrole-4-carbonyl) butyl]amide
3-?henylpyrrole-l-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyrrole -4-carbonyl) butyljamide
^-[l-(3-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl] benzamide
4-fe7t-Butyl-A^[l-(3-hydroxybenzyl)-2-oxo-2-(3-oxo-liexahydroruro[3,2-i]pyrrol-4-yl)ethyl]benzamide
Ar-[l-(3-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[352-6]pyrrol-4-yl)ethyl]-4-trifluoromethoxybenzamide

4-Dime%lamino-//-[l-(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-£]pyrrol-4-yl)ethyl]benzamide
^V-[l-(3-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-^]pyrrol-4-yl)ethyl]-4-isopropylbenzamide
4-Difluoromethoxy-JV-[l-(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide
AT-[l-(3-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]-4-trifluoromethylbenzamide
4-Bromo-JV'-[l-(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-Z?]pyrrol-4-yl)ethyl]benzamide
3-Bromo-Ar-[l-(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide
2V-[l-(3-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofiaro[3,2-6]pyrrol-4-yl)ethyl]-4-vinylbenzamide
Naphthalene-2-carboxylic acid [1 -(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-fe]pyTrol-4-yl)ethyl]amide
Naphthalene- 1-carboxylic acid [l-(3-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofiiro [3 ^2-6]pyrrol-4-yl)ethyl]amide
Quinoline-6-carboxylic acid [1 -(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]amide
Benzo[6]thiophene-2-carboxylic acid [1 -(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa hydrofiiro[3,2-Z)]pyrrol-4-yl)ethyl]amide
Benzo[6]thiophene-3-carboxylic acid [ 1 -(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro [3,2-6]pyirol-4-yl)ethyl]amide
Benzothiazole-5-carboxylic acid [1 -(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydro fiiro[3,2-fe]pyrrol-4-yl)ethyl]amide
Biphenyl-4-carboxylic acid [l-(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-i]pyrrol-4-yl)ethyl]amide
JV-[l-(3-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-phenoxybenzamide
AT-[l-(3-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-i]pyrrol-4-yl)ethyl]-3-phenoxybenzamide
^V-Cl-(3-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-63pyrrol-4-yl)ethyl]-4-imidazol-1 -ylbenzamide
7/-[l-(3-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-thiophen-2-ylbenzainide
A^-[l Ar-[l-(3-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fc]pyrrol-4-yl)ethyl]-4-[ 1 }2,3]thiadiazol-5-ylbenzamide
yV-[l-(3-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]-4-pyrazol-1 -ylb enzamide
A^[l-(3-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)et;hyl]-5-thiophen-2-ylnicotinamide
A^-[l-(3-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-6-phenylnicotinamide
2-Phenylthiazole-4-carboxylic acid [1 -(3-hydroxyben2yl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-&]pyrrol-4-yl)ethyl]amide
2-Pyridin-3-ylthiazole-4-carboxylic acid [l-(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
5-Phenylthiophene-2-carboxylic acid [ 1 -(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-Z>]pyrrol-4-yl)ethyl]amide
5-Pyridin-3-yltbiophene-2-carboxylic acid [1 -(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
2-Methyl-5-phenylfuran-3-cafboxylic acid [1 -(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)eihyl]amide
4-Phenylthiophene-2-carboxylic acid [1 -(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
4-Pyridin-3-ylthiophene-2-carboxylic acid [l-(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydroforo[3,2-6]pyrrol-4-yl)etliyl]amide
2-Thiophen-2-ylthiazole-4-carboxylic acid [ 1 -(3-hydroxybenzyl)-2-oxo-2-(3 -oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
2-Methyl-5-(pyrrolidine-l-sulfonyl)furan-3-carboxylic acid [l-(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
3-Phenylpyrrole-l-carboxylic acid [l-(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydro iuro[3,2-6]pyrrol-4-yl)ethyl] amide
Ar-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-4-ylmethylethyl] benzamide
4-/ferr-Butyl-JV-[2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]benzamide
7^-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]-4-trifluoromethoxybenzamide
4-Dimethylamino-JV-[2-oxo-2-(3-oxo-hexahydrofiu>o[3,2-&]pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]benzamide
^-[2-Oxo-2-(3-oxo-hexahydrofco[3,2-6]pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]-4-isopropylbenzamide
4-Difluoromethoxy-/^-[2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]benzamide
A^[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)-l-pyridin-4-ylmetliylet:hyl]-4-trifluoromethylbenzamide
4-Bromo-Ar-[2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]benzamide
3-Bromo-//-[2-oxo-2--(3-oxo-hexahydroftiro[3)2-6]pyrrol-4-yl)-l-pyridin-4-yl methylethyljbenzamide
A7-[2-Oxo-2-(3-oxo-hexahydrofuro[332-6]pyrrol-4-yl)-l-pyridin-4-ylmet;hylethyl]-4-\dnylbenzamide
Naphthalene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]amide
Naphthalene-1-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-4-yknethylethyl]amide
Quinoline-6-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[352-£]pyrrol-4-yl)-l -pyridin-4-ylmethylethyl]amide
Benzo[6]thiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-1 -pyridin-4-yhnethylethyl] amide
Benzo[fe]thiophene-3-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3^-6]pyrrol-4-yl)-1 -pyridin-4-yhnethylethyl]amide
Benzotniazole-5-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-1 -pyridin-4-ylinethylethyl]amide
Biphenyl-4-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3 s2-i>]pyrrol-4-yl)-1 -pyridin-4-ylmethylethyl]amide
AT-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-4-yhnethylethyl]-4-phenoxybenzamide
Ar-[2-Oxo-2-(3-oxo-hexahydrofoo[3,2-fe]pyrrol-4-yl)-l-pyridin-4-ylinethyletliyl]-3-phenoxybenzamide
[3,2-fe]pyrrol-4-yl)-l-pyridin-4-ylmetliylethyl]-4-imidazol-1 -ylbenzamide
Ar-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-i]pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]-4-thiophen-2-ylbenzamide
A^-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-fc]pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]-4-oxazol-5-ylbenzamide
jV-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyiTol-4-yl)-l-pyridin-4-ylinethyleihyl]-4-[ 1,2,3]thiadiazol-5-ylbenzainide
Ar-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrroM-yl)-l-pyridin-4-ylmethylethyl]-4-pyrazol-1 -ylbenzamide
Ar-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-1 -pyridm-4-ylmethylethyl j-5-thiophen-2-ylnicotinamide
AT-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]py]Tol-4-yl)-l-pyridin-4-ylmel3iyletiiyl]-6-phenylnicotinamide
2-Phenylthiazole-4-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-1 -pyridin-4-ylmethylethyl]amide
2-Pyridin-3-ylthiazole-4-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6] pyrrol-4-yl)-l-pyridin-4-ylmetb.ylethyl]amide
5-Phenylthiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-i]pyrrol-4-yl)-1 -pyridin-4-ylmethylethyl]amide
5-Pyridin-3-ylthiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]amide
2-Methyl-5-phenylfuran-3-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe] pyrrol-4-yl)- 1 -pyridin-4-ylmethylethyl] amide
4-Phenylthiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l -pyridin-4-ylmethylethyl]amide
4-Pyridin-3-ylthiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-&] pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]amide
2-Thiophen-2-ylthiazole-4-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofiiro[3,2-fc] pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]amide
2-Methyl-5-(pyrrolidine-l-sulfonyl)furan-3-carboxylic acid [2-oxo-2-(3-oxo-b.exa hydrofuro[3,2-i]pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]arnide
3-Phenylpyrrole-l -carboxylic acid [2-oxo-2-(3-oxo-liexahydrofuro[3,2-&]pyrrol-4-yl)-l-pyridin-4-ylmethylethyl]amide
benzamide
4-te^Butyl-^-[2-oxo-2 trifluoTomethoxybenzamide
4-Dimethylamino-^-[2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-3-ylmethylethyl]benzamide
A^-[2-Oxo-2-(3-oxo-hexahydrofuro[352-i]pyrrol-4-yl)-l-pyridin-3-ylmethylethyl]-4-isopropylbenzamide
4-Difluoromethoxy-A^[2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-3-ylmethylethyl]benzamide
A^-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)-l-pyridin-3-yhnethylethyl]-4-trifluoromethylbenzainide
4-Bromo-JV-[2-oxo-2-(3 -oxo-hexahy drofuro[3,2-6]pyrrol-4-yl)-1 -pyridin-3-yhnethylethyl]benzamide
3-Bromo-7V-[2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)-l-pyridin-3-yhnethylethyljbenzamide
JV"-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-Z?]pyrrol-4-yl)-l-pyridin-3-yknethylethyl]-4-vinylbenzamide
Naphthalene-2-cafboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)-l-pyridm-3-ylmethylethyl]amide
Naphthalene-1-carboxyUc acid [2-oxo-2-(3-oxo-hexahydroiuro[3J2-6]pyrrol-4-yl)-l-pyridin-3-yhnethylethyl]amide
Quinoline-6-cafboxylic acid [2-oxo-2-(3 -oxo-hexahydrofuro [3,2-Z»]pyrrol-4-yl)-1 -pyridin-3-ylmethylethyl]amide
Benzo[6]thiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofwo[3,2-6]pyrrol-4-yl)-l -pyridin-3-yknethylethyl]amide
Benzo[6]tbiophene-3-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol--pyridin-3-yhnefhylethyl]amide
Benzothia2ole-5-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3s2-6]pyrrol-4-yl)-1 -pyridin-3-ylmethylethyl]amide
Biphenyl-4-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofviro[3,2-6]pyrrol-4-yl)-l -pyridin-3 -ylmethylethyljamide
A^-[2-Oxo-2-(3-oxo-hexahydrofuro[3J2-6]pyrrol-4-yl)-l-pyridin-3-ylmethylethyl]-4-phenoxybenzamide
Ar-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-3-ylmethyletliyl]-3-phenoxybenzamide
W-[2-Oxo-2-(3-oxo4iexahydrofuro[3,2-&]pyrroM imidazol-1 -ylbenzamide
JV"-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyiroW-yl)4-pyridin-3-ylmethylethyl]-4-thiophen-2-ylbenzamide
7^[2-Oxo-2-(3-oxo-hexahydroforo[3>6]pyrroW-yl)-l-pyridin-3-ylmethylethyl]-4-oxazol-5-ylbenzamide
7vr-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-3-ylmethylethyl]-4-[ 1,2,3]thiadiazol-5-ylbenzamide
Ar-[2-Oxo-2-(3-oxo-hexahydrofiiro[3,2-6]pyrrol-4-yl)-l-pyridia-3-ylniethylethyl]-4-pyrazol-1 -ylbenzamide
A'-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-3-ylmethylethyl]-5-tbiophen-2-ylnicotinamide
7\/-[2-Oxo-2-(3-oxo-hexahy(kofuro[3,2-i]pyrrol-4-yl)-l-pyridin-3-ylmethylet:hyl]-6-phenylnicotinamide
2-Phenylthiazole-4-carboxylic acid [2-oxo-2-(3-oxo-hexahydroforo[3 ,2-&]pyrrol-4-yl)- 1 -pyridin-3 -ylmethylethyl]amide
2-Pyridin-3-ylthiazole-4-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6] pyrrol-4-yl)-l-pyridin-3-ylmethylethyl]amide
5-Phenylthiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)- 1 -pyridin-3 -ylmethylethyl] amide
5-Pyridin-3-ylthiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-)- 1 -pyridin-3-ylmethylethyl]amide
2-Methyl-5-phenylfuran-3-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-&] pyrrol-4-yl)-l-pyridin-3-ylmethylethyl]amide
4-Pb.enylthiophene-2-cafboxylic acid [2-oxo-2-(3-oxo-hexah.ydrofuro[3,2-6]pyrrol-4-yl)- 1 -pyridin-3-ylmethylefliyl]amide
4-Pyridin-3-ylthiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6] pyrrol-4-yl)- 1 -pyridin-3-ylmethylethyl]amide
2-Thiophen-2-ylthiazole-4-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6] pyrrol-4-yl)-l-pyridin-3-ybiiethyletliyl]amide
2-Methyl-5-(pyrrolidine-l-sulfonyl)furan-3-carboxylic acid [2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)-l-pyridin-3-yknethylethyl]amide
3-Phenylpyrrole-l-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)-l -pyridin-3-ylmethylethyl] amide
A'.[l_(4-Bromobenzyl)-2-oxo-2-(3-oxo-hexaiiydrofuro[3,2-6]pyrrol-4-yl)ethyl] benzamide
4-re7^-Butyl-JV-[l-(4-bromoben2yl)-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-&]pyrrol-4-y 1) ethyl]benzamide
Ar.[ 1 .(4-Bromobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]-4-trifluoromethoxybenzamide
4-Dime1hylamino-Ar.[l-(4-broinobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2--Z?]pyrrol-4-yl)ethyl]benzamide
A-[l-(4-Bromobenzyl)-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-&]pyrrol-4-yl)ethyl]-4-isopropylbenzamide
4-Difluoromethoxy-JV'-[l-(4-bromobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide
^-[l-(4-Bromobenzyl)-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-fc]pyirol-4-yl)ethyl]-4-trifluoromethylbenzamide
4-Bromo-Ar-[l-(4-bromoben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[352-6]pyrrol-4-yl)ethyl]benzamide
3-Bromo-7V'-[l-(4-bromoben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)ethyl]benzamide
Ar-[l-(4-Bromobenzyl)-2-oxo-2-(3-oxo-liexaliydrofuro[3,2-&]pyrrol-4-yl)ethyl]-4-vinylbenzamide
Naphthalene-2-carboxylic acid [1 -(4-bromobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-fe]pyrrol-4-yl)ethyl]amide
Naphthalene-1 -cafboxylic acid [l-(4-bromobenzyl)-2-oxo-2-(3-oxo-hexahydrof\iro [3,2-6]pyrrol-4-yl)ethyl]amide
Quinoline-6-carboxylic acid [ 1 -(4-bromobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-&]pyrrol-4-yl)ethyl]aimde
Benzo[6]thiophene-2-carboxylic acid [l-(4-bromobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-&]pyrrol-4-yl)ethyl]amide
Benzo[6]thiophene-3-carboxylic acid [1 -(4-bromobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-£>]pyrrol-4-yl)ethyl]amide
Benzotbiazole-5-carboxylic acid [l-(4-bromoben2yl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-^]pyrrol-4-yl)ethyl]amide
Bipb.enyl-4-carboxylic acid [l-(4-bromoben2yl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-fe]pyrrol-4-yl)ethyl]ainide
^-[l-(4-Bromoben2yl)-2-oxo-2 A^-[l-(4-Bromobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fc]pyrrol-4-yl)ethyl]-3-phenoxybenzamide
A/"-[l-(4-Bromobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]-4-imidazol-1-ylbenzamide
7V-[l-(4-Bromobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3J2-6]pyrrol-4-yl)ethyl]-4-thiophen-2-ylberizamide
N-[l-(4-Bromobenzyl)-2-oxo-2-(3-oxo-hexahydroftiro[3,2-6]pyrrol-4-yl)ethyl]-4-oxazol-5 -ylbenzamide
7^-[l-(4-Bromoben2yl)-2-oxo-2-(3-oxo-liexahydrofuro[3,2-fe]pyrrol-4-yl)ethyl]-4-[1,2,3]thiadiazol-5-ylbenzainide
vV-[l-(4-Bromobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]-4-pyrazol-1 -ylbenzamide
Ar-[l-(4-Bromobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-5-thiophen-2-ylnicotinamide
N-[ 1 -(4-Bromoben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-6-phenylnicotinamide
2-Phenylthiazole-4-carboxylic acid [l-(4-bromobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-£>]pyrrol-4-yl)ethyl]amide
2-Pyridin-3-ylthia2ole-4-carboxylic acid [l-(4-bromobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-i]pyiTol-4-yl)ethyl]anude
5-Phenylthiophene-2-carboxylic acid [l-(4-bromobenzyl)-2-oxo-2-(3-oxo-liexa hydrofuro[332-fe]pyrrol-4-yl)ethyl]ainide
5-Pyridin-3-ylthiopheae-2-carboxylic acid [1 -(4-bromobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-Z)]pyrrol-4-yl)ethyl]aniide
2-Methyl-5-plienylfuran-3-carboxylic acid [1 -(4-bromobenzyl)-2-oxo-2-(3-oxo-hexah.ydrofuro[3,2-fe]pyrrol-4-yl)ethyl]amide
4-PhenyIthiophene-2-carboxyIic acid [1 -(4-bromobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
4-Pyridin-3-ylthiophene-2-carboxylic acid [1 -(4-bromobenzyl)-2-oxo-2-(3-oxo-hexahydroftiro[3,2-fr]pyrrol-4-yl)ethyl]ainide
2-Thiophen-2 -ylthiazole-4-carboxylic acid [l-(4-bromobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]amide
2-Methyl-5-(pyrrolidine-l-sulfonyl)furan-3-carboxylic acid [l-(4-bromoben2yl)-2-oxo-2-(3-oxo-hexahydroftiro[3,2-i]pyrrol-4-yl)ethyl]amide
3-Phenylpyrrole-l-carboxylic acid [1 -(4-bromobenzyl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-6]pyrrol-4-yl)ethyl]amide
Ar-[l-(4-Fluorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)eihyl] benzamide
4-fer^Butyl-7/-[l-(4-fluoroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide
Ar-[l-(4-Fluoroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3J2-6]pyrrol-4-yl)ethyl]-4-trifluoromethoxybenzamide
4-Dimethylamino-JV-[l-(4-fluorobenzyl)-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-i]pyrrol-4-yl)ethyl]benzamide
7/-[l-(4-Fliiorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)et;hyl]-4-isopropylbenzamide
4-Difluoromethoxy-7V-[l-(4-fluorobenzyl)-2-oxo-2-(3-oxo-liexaliydrofuro[3,2-£j]pyrrol-4-yl)ethyl]benzamide
Ar-[l-(4-Fluoroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-trifluoromethylbenzamide
4-Bromo-Ar-[l-(4-fluoroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-Z?]pyrrol-4-yl)ethyl]benzamide
3-Bromo-A;'-[l-(4-fluorobeii2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3s2-6]pyrrol-4-yl)ethyl]benzamide
A^[l-(4-Fluorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)eihyl]-4-\inylbenzamide
"Naphthalene-2-carboxylic acid [1 -(4-fluoroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]amide
Naphthalene-1-carboxylic acid [l-(4-fluoroben2yl)-2-oxo-2-(3-oxo-hexah.ydrofuro [3,2-fe]pyrrol-4-yl)ethyl]amide
Quinoline-6-carboxylic acid [l-(4-fluorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-i]pyrrol-4-yl)ethyl] amide
Benzo[6]thiophene-2-carboxylic acid [l-(4-fluorobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)ethyl]aniide
Benzo[fe]thiophene-3-carboxylic acid [l-(4-fluorobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
Benzothiazole-5-carboxylic acid [l-(4-fluorobenzyl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-fe]pyrrol-4-yl)ethyl]amide
Biphenyl-4-carboxylic acid [l-(4-fluoroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]amide .
^/•.[l.(4_FlUorobenzyl)-2-oxo-2-(3-oxo-hexahydroiuro[3,2-Z)]pyrrol-4-yl)ethyl]-4-phenoxybenzamide
AT-[l-(4-Fluorobenzyl)-2-oxo-2-(3-oxo-hexabydrofuro[352-fc]pyrrol-4-yl)ethyl]-3-phenoxybenzamide
7^-[l-(4-Fluorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-b]pyrrol-4-yl)et:hyl]-4-imidazol-1 -ylbenzamide
JV-[l-(4-Fluorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-thiophen-2-ylben2amide
//-[l-(4-Fluorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3J2-6]pyrrol-4-yl)ethyl]-4-oxazol-5-ylbenzamide
^-[l 7/-[l-(4-Fluoroben2yl)-2-oxo-2-(3-oxo-hexahydroiuro[3,2-fe]pyrrol-4-yl)ethyl]-4-pyrazol-1 -ylbenzamide
JV-[l-(4-Fluoroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3J2-6]pyrrol-4-yl)eihyl]-5-tbiophen-2-ylnicotinainide
JV-[l-(4-Fluorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]-6-phenylnicotinamide
2-Phenylthiazole-4-carboxylic acid [1 -(4-fluorobenzyl)-2-oxo-2-(3-oxo-hexa hydrofiiro[3,2-6]pyrrol-4-yl)ethyl]amide
2-Pyridin-3-ylthiazole-4-carboxylic acid [1 -(4-fluorobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
5-Phenylthiophene-2-carboxylic acid [l-(4-fl.uorobenzyl)-2-oxo-2-(3-oxo-liexa hydrofuro[3,2-6]pyrrol-4-yl)ethyl]atnide
5-Pyridin-3-yliMophene-2-carboxylic acid [l-(4-fluorobenzyl)-2-oxo-2-(3-oxo-hexahydroforo[3,2-&]pyrrol-4-yl)ethyl]amide
2-Methyl-5-phenylfuran-3-carboxylic acid [l-(4-fluorobenzyl)-2-oxo-2-(3-oxo-hexahydrofi3ro[3,2-6]pyrrol-4-yl)et:hyl]amide
4-Phe«yltbiophene-2-carboxylic acid [l-(4-fluoroben2yl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-i]pyrrol-4-yl)ethyl]amide
4-Pyridin-3-ylthiophene-2-cafboxylic acid [l-(4-fluorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)ethyl]amide
2-Tbiophen-2-ylthiazole-4-cafboxylic acid [l-(4-fiuoroben2yl)-2-oxo-2-(3-oxo-b.exahydrofuro[3,2-Z)]pyrrol-4-yl)eihyl]amide
2-Methyl-5-(pyrrolidine-l-sulfonyl)furan-3-carboxylic acid [l-(4-fluorobenzyl)-2-oxo-2-(3-oxo-b.exahydrof\aro[3}2-6]pyrrol-4-yl)ethyl]amide
3-Phenylpyrrole-l-carboxylic acid [l-(4-fluorobenzyl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-fe]pyrrol-4-yl)ethyl]amide
jV-[l -(4-Chlorobenzyl)-2-oxo-2-(3 -oxo-hexahydroforo[3,2-6]pyrrol-4-yl)ethyl] benzamide
yl)ethyl]benzamide
JV-[l-(4-Chlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-trifluoromethoxybenzamide
4-Dimethylamino-JV-[l-(4-chloroben2yl)-2-oxo-2-(3-oxo-hexaliydrojEuro[3,2-6]pyrrol-4-yl)ethyl]benzamide
^-[l-(4-Chlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofiiro[3,2-&]pyrrol-4-yl)ethyl]-4-isopropylbenzamide
4-Difluoromethoxy-JV-[l-(4-chlorobenzyl)-2-oxo-2-(3-oxo-hexaliydrofuro[332-i]pyrrol-4-yl)ethyl]benzamide
7^-[l-(4-CMoroben2yl)-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-%yrrol-4-yl)ethyl]-4-trifluoroinethylbenzaniide
4-Bromo-7/-[l-(4-chlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide
3-Bromo-JV"-[l-(4-cbloroben2yl)-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-fe]pyrrol-4-yl)ethyl]benzaniide
7^-[l-(4-Chloroberi2yl)-2-oxo-2-(3-oxo-hexahydrofuro[332-Z)]pyrrol-4-yl)ethyl]-4-vinylbenzaniide
Naphthalene-2-carboxylic acid [ 1 -(4-chloroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]amide
Naphthalene-1-cafboxylic acid [l-(4-chlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]amide
Quinoline-6-cafboxylic acid [1 -(4-chlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3 ,2-6]pyrrol-4-yl)ethyl]amide
Benzo[6]thiophene-2-carboxylic acid [l-(4-chlorobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-&]pyrrol-4-yl)etbyl]amide
Benzo[6]thiophene-3-carboxylic acid [1 -(4-chloroben2yl)-2-oxo-2-(3-oxo-hexa hydro furo[3,2-6]pyrrol-4-yl)ethyl]amide
Benzothiazole-5-carboxylic acid [1 -(4-chlorobenzyl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-6]pyrrol-4-yl)ethyl]amide
Biphenyl-4-carboxylic acid [l-(4-chloroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro 6]pyrrol-4-yl)eihyl]aniide
AT-[l-(4-CUorobenzyl)-2-oxo-2-(3-oxo-hexahydroftu:o[3,2-fe]pyrrol-4-yl)ethyl]-4-phenoxybenzamide
JV"-[l-(4-(^orobeii2yl)-2-oxo-2-(3-oxo-hexahydroftiro[3,2-fc]pyiToW-yl)ethyl]-^ phenoxybenzamide
A^[l-(4-CWoroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-imidazol- 1 -ylbenzamide
7V-[l-(4rChlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl3-4-thiophen-2-ylbenzamide
^-[l-(4-Chloroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-oxazol-5-ylbenzamide
_?V.[l.(4-Chlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-[1,2,3]thiadiazol-5-ylbenzainide
A^-[l-(4-Chlorobenzyl)-2-oxo-2-(3-oxo-hexahydroftiro[3,2-6]pyrrol-4-yl)ethyl]-4-pyrazol-1 -ylbenzamide
AT.[i_(4.chlorobenzyl)-2-oxo-2-(3-oxo-hexaliydroforo[3,2-6]pyrrol-4-yl)ethyl]-5-thiophen-2-ylnicotinamide
A^[l-(4-Chlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-6-phenylnicotinamide
2-Phenylthiazole-4-carboxylic acid [1 -(4-chlorobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-fc]pyrrol-4-yl)etb.yl]amide
2-Pyridin-3-ylthiazole-4-cai-boxylic acid [ 1 -(4-chlorobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-fe]pyrrol-4-yl)ethyl]amide
5-Phenyltbiophene-2-carboxylic acid [ 1 -(4-chlorobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
5-Pyridin-3-ylthiopliene-2-carboxylic acid [l-(4-cblorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-i]pyrrol-4-yl)ethyl]amide
2-Methyl-5-phenylfuran-3-carboxylic acid [l-(4-chloroben2yl)-2-oxo-2-(3-oxo-b.exahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
4-Phenyltbiophene-2-carboxylic acid [1 -(4-chloroben2yl)-2-oxo-2-(3-oxo-hexa hydrofuro[352-6]pyirol-4-yl)ethyl]arnide
4-Pyridin-3-yltbiophene-2-carboxylic acid [1 -(4-chlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-i]pyrrol-4-yl)ethyl]amide
i-Thiophen-2-ylthiazole-4-carboxylic acid [1 -(4-chlorobenzyl)-2-oxo-2-(3-oxo-iexahydrofuro[3,2-6]pyrrol-4-yl)eihyl]amide
2-Methyl-5-(pyrrolidiae-l -sulfonyl)furan-3-carboxylic acid [ 1 -(4-chlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)ethyl]amide
3-Phenylpyrrole-1 -carboxylic acid [1 -(4-chloroben2yl)-2-oxo-2-(3 -oxo-hexahydro furo[3,2-6]pyrrol-4-yl)ethyl]amide
7^-[l-(3,4-DicUorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[352-Z)]pyrrol-4-yl)ethyl] benzamide
4-fert-Butyl-^[l-(3J4-dichlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3J2-fc]pyrrol-4-yl)ethyl]benzamide
Ar-[l-(3,4-Dichloroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)eihyl]-4-trifl.uoromethoxybenzamide
4-Dimeihylamino-N'-[l-(3s4-dicUoroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide
JV-[l-(3,4-Dichloroben2yl)-2-oxo-2-(3-oxo-liexaliydrofliro[3,2-i]pyrrol-4-yl)ethyl]-4-isopropylbenzamide
4-Difluoromeflioxy-JV-[l-(3,4-dichlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide
^-[l-(3,4-DicMoroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyirol-4-yl)ethyl]-4-trifluoromethylben2amide
4-Bromo-W-[l-(3,4-dichlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide
3-Bromo-7\T-[l-(3,4-diclilorobenzyl)-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide
^.[i_(3)4-Dichlorobenzyl)-2-oxo-2-(3-oxo-liexahydrofuro[3)2-6]pyrrol-4-yl)ethyl]-4-vinylbenzamide
Naphthalene-2-carboxylic acid [1 -(3,4-dichlorobenzyl)-2-oxo-2-(3-oxo-
a,
hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]amide
Naphthalene-1-carboxylic acid [l-(354-dichlorobenzyl)-2-oxo-2-(3-oxo-
hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
Quinoline-6-carboxylic acid [1 -(3,4-dichlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]amide
Benzo[Z?3thiophene-2-carboxylic acid [1 -(3,4-dichlorobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-&]pyrrol-4-yl)ethyl]amide
Benzo[6]thiophene-3-carboxylic acid [1 -(3,4-dicUorobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
Benzothiazole-5-carboxylic acid [l-(3,4-dichlorobenzyl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-fe]pyrrol-4-yl)ethyl]amide
Biphenyl-4-carboxylic acid [1 -(3,4-dichlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [352-6]pyrrol-4-yl)ethyl]amide
A^-[l-(3,4-Dichloroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-phenoxybenzamide
iV-[l-(3,4-Dichlorobenzyl)-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-6]pyrrol-4-yl)ethyl]-3 -phenoxybenzamide
A-[l A^[l2-6]pyrrol-4-yl)ethyl]-4-thiophen-2-ylbenzamide
A:-[l-(3,4-Dichlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3J2-6]pyrrol-4-yl)ethyl]-4-oxazol-5 -ylbenzamide
A'-[l-(3J4-Dichlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)efhyl]-,2,3]tbiadiazol-5-ylbenzamide
Ar-[l-(3,4-Dichloroben2yl)-2-oxo-2-(3-oxo-hexahydrofurot3,2-fe]pyrrol-4-yl)ethyl]-4-pyrazol-l -ylbenzamide
Ar-[l-(3J4-Dichlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofbro[3>2-6]pyrrol-4-yl)ethyl]-5-thiophen-2-ylnicotinamide
Ar-[l-(3,4-Dichlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)ethyl]-6-phenylnicotinamide
2-Phenylthiazole-4-carboxylic acid [l-(3,4-dichlorobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-i]pyrrol-4-yl)ethyl]amide
2-Pyridin-3-ylthiazole-4-carboxylic acid [l-(3,4-dichlorobenzyl)-2-oxo-2-(3-oxo-hexahydrofhro[3,2-6]pyrrol-4-yl)ethyl]amide

5-Phenylthiophene-2-carboxylic acid [l-(3,4-dichlorobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-63pyrrol-4-yl)ethyl]ainide
5-Pyridin-3-ylthiophene-2-carboxylic acid [l-(3,4-dichlorobenzyl)-2-oxo-2-(3-oxo-b.exahydrofuro[3,2-6]pyrrol-4-yl)etb.yl]amide
2-Methyl-5-phenylfuran-3-carboxylic acid [ 1 -(3,4-dichloroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
4-Phenylthiophene-2-carboxylic acid [ 1 -(3,4-dichloroben2yl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-fe]pyrrol-4-yl)ethyl]ainide
4-Pyridin-3-yltbiophene-2-carboxylic acid [l-(3,4-dichloroben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-^]pyrrol-4-yl)ethyl]ainide
2-Tbiophen-2-ylthiazole-4-carboxylic acid [ 1 -(3,4-dichlorobenzyl)-2-oxo-2-(3-oxo-b.exahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
2-Methyl-5-(pyrrolidine-l-sulfonyl)furan-3-carboxylic acid [1 -(3,4-dichlorobenzyl)-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-£»]pyrrol-4-yl)ethyl]amide
3-PhenyIpyrrole-l-carboxylic acid [l-(3,4-dichlorobeii2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-i]pyrrol-4-yl)ethyl]amide
Ar-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-thiophen-2-ylmethylethyl] benzamide
4-re7'NButyl-^-[2-oxo-2-(3-oxo-hexahydrofuro[3,2-%yirol-4-yl)-l-thiophen-2-ylmethylethyljbenzamide
Ar-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-i]pyrrol-4-yl)-l-thioplien-2-yhnethylethyl]-4-trifluoroinethoxybenzainide
4-Dime%lamino-^-[2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)-l-thiophen-2-ylmethylethyl]benzamide
AT-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-thiophen-2-ylmethylethyl]-4-isopropylbenzamide
4-Difluoromethoxy-^-[2-oxo-2-(3-oxo-hexahydrofuro[352-fe]pyrrol-4-yl)-l-tbiophen-2-ylmethyleihyl]benzainide
Ar-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)-l-thiophen-2-ylmethylethyl]-4-trifluoromethylbenzamide
4-Bromo-7V"-[2-oxo-2-(3-oxo-hexahydrofuro[352-6]pyrrol-4-yl>l-tbiophen-2-ylmethylethyl]benzamide
3-Bromo-J\T-[2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-thiopheii-2-ylmethylethyl]benzamide
Ar-[2-Oxo-2 Naphthalene-2-cafboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)-1 -thiophen-2-ylmethylethyl]amide
Naphthalene-1-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)-l-thiophen-2-ybiethylethyl]ainide
Quinoline-6-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)-l-thiophen-2-ylmethylethyl]amide
Benzo[fc]thiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-Z?]pyrrol-4-yl)- 1 -thiophen-2-ylmethylethyl] amide
Benzo[fe]thiophene-3-carboxylic acid [2-oxo-2-(3-oxo-hexahydroforo[3,2-6]pyrrol-4-yl)- 1 -thiophen-2-ylmethylethyl]amide
Benzothiazole-5-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-1 -thiophen-2-ylmethylethyl]amide
Biphenyl-4-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3 ,2-6]pyrrol-4-yl)- 1 -thiophen-2-ylmethylethyl]amide
//-[2-Oxo-2-(3-oxo-hexahydroforo[3,2-6]pyrrol-4-yl)-l-tbiophen-2-ylmethylethyl]-4-phenoxybenzamide
3-phenoxybenzamide
A^[2-Oxo-2 A^[2-Oxo-2 AT-[2-Oxo-2 ^-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)-l-thiophen-2-ylmethylethyl]-4-[ 1 ,2,3]thiadiazol-5-ylbenzamide
^-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrroI-4-yl)-l-thiophen-2-ylmethylethyl]-4-pyrazol-l -ylbenzamide
Ar-[2-Oxo-2-(3-oxo-hexah.ydrofuro[3,2-fe]pyrrol-4-yl)-l-thiophen-2-ylmethyleihyl]-5-tbiophen-2-ylrucotinarnide
jY-[2-Oxo-2-(3-oxo-hexahydrofuro[3J2-fe]pyrrol-4-yl)-l-thiopheri-2-ylmethylethyl]-6-phenylnicotinarnide
2-Phenylthiazole-4-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyirol-4-yl)-1 -thiophen-2-ylmethylethyl]amide
2-Pyridin-3-ylthiazole-4-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-b] pyrrol-4-yl)-1 -lMophen-2-ylmethylethyl]amide
5-PhenyltMopriene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[352-b]pyrrol-4-yl)-1 -thiophen-2-ylmethylethyl]amide
5-Pyridin-3-yltbiopb.ene-2-carboxylic acid [2-oxo-2-(3-oxo-b.exah.ydrofuro[3,2-6]pyrrol-4-yl)-1 -thiophen-2-ylmethylethyl]ainide
2-Methyl-5-phenylfuran-3-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6] pyrrol-4-yl)-l-thiophen-2-ylmeihylethyl]amide
4-Phenylthiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-1 -tbiophen-2-ylmethylethyl]amide
4-Pyridin-3-ylthiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexah.ydrofuro[3,2-b] pyrrol-4-yl)-l-thiophen-2-ylmethylethyl]amide
2-Thiophen-2-ylthiazole-4-carboxylic acid [2-oxo-2-(3-oxo-hexaliydrofuro[3,2-6] pyrrol-4-yl)-1 -thiophen-2-ylraethylethyl]amide
2-Methyl-5-(pyrrolidine-l-sulfonyl)furan-3-carboxylic acid [2-oxo-2-(3-oxo-hexa hydrofuro[3,2-Z>]pyrrol-4-yl)-l -thiophen-2-ylmethylethyl] amide
3-Phenylpyrrole-l-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-%yrrol-4-yl)-1 -thiophen-2-ylmethylethyl]amide
A*-[l-(4-Aminobenzyl)-2-oxo-2-(3-oxo-hexaliydrofuro[3>2-6]pyrrol-4-yl)ethyl] benzamide
4-re^Butyl-N"-[l-(4-aminobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide
7/-[l-(4-Aiiiinoben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)e^ trifluoromethoxybenzainide
4-Dimethylamino-JV"-[l-(4-aminobenzyl)-2-oxo-2-(3-oxo-b.exahydrofuro[3,2-fc]pyrrol-4-yl)ethyl]benzamide
A^-[l-(4-Aminobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl3-4-isopropylbenzamide
4-Difluoromethoxy-JV-[l-(4-aminobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide
^-[l-(4-Aminobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-trifluoromeihylbenzamide
4-Bromo-^-[l-(4-aminobenzyl)-2-oxo-2-(3-oxo-hexah.ydrofuro[3,2-6]pyrrol-4-yl)ethyl]ben2;amide
3-Bromo-7V"-[l-(4-amiiiobenzyl)-2-oxo-2-(3-oxo-hexahydrofijro[3,2-fe3pyrrol-4-yl)ethyl]benzamide
N-[ 1 -(4-Aminobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-vinylbenzamide
Naphthalene-2-carboxylic acid [ 1 -(4-aminobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3.2-&]pyrrol-4-yl)ethyl]amide
Naphthalene-1-carboxylic acid [l-(4-aminoben2yl)-2-oxo-2-(3-oxo-hexahydrofuro [3.2-fe]pyrrol-4-yl)ethyl]amide
Quinoline-6-carboxylic acid [1 -(4-aminobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3.2-fc]pyrrol-4-yl)ethyl] amide
Benzo[fc]tbiophene-2-carboxylic acid [l-(4-ammobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyirol-4-yl)ethyl]amide
Benzo[fe]tbiophene-3-carboxylic acid [l-(4-aminobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3^2-6]pyrrol-4-yl)ethyl]amide
Benzothiazole-5-carboxylic acid [l-(4-aminoben2yl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-^]pyrrol-4-yl)ethyl]amide
Bipb.enyl-4-carboxylic acid [l-(4-aminobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]amide
AT-[l-(4-Atninobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-phenoxybenzamide
A7-[l-(4-Aminoben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]-3-phenoxybenzamide
N-[ 1 -(4-Aminoben2yl)-2-oxo-2-(3 -oxo-hexahydrofuro [3,2-2>]pyrrol-4-yl)ethyl]-4-imidazol-l-ylbenzamide
A'"-[l-(4-Aminobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-thiophen-2-ylbenzamide
A7-[l-(4-Aminoben2yl)-2-oxo-2-(3-oxo-liexahydroftiro[3,2-Z)]pyrrol-4-yl)ethyl]-4-oxazol-5-ylbenzamide
Ar-[l-(4-Aininobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-[1,2,3]thiadiazol-5-ylbenzainide
Ar-[l-(4-Aminobeii2yl)-2-oxo-2-(3-oxo-hexahydrofiiro[3,2-6]pyrrol-4-yl)ethyl]-4-pyrazol-1 -ylbenzamide
Ar-[l-(4-Aminoben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-5-tbiophen-2-ylnicotinamide
Ar-[l-(4-Aminobeiizyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)ethyl]-6-phenylnicotinamide
2-Phenylihiazole-4-carboxylic acid [l-(4-aminobenzyl)-2-oxo-2-(3-oxo-hexa hydrofUro[3s2-Z?]pyrrol-4-yl)ethyl]amide
2-Pyridin-3-ylthiazole-4-carboxylic acid [l-(4-aminobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-63pyrrol-4-yl)ethyl]amide
5-Phenylthiophene-2-carboxylic acid [l-(4-aminobenzyl)-2-oxo-2-(3-oxo-hexa hydroiuro[3,2-2?]pyrrol-4-yl)ethyl]amide
5-Pyridin-3-ylthiophene-2-carboxylic acid [l-(4-aminoben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-i]pyrrol-4-yl)ethyl]amide
2-Methyl-5-phenylfuran-3-carboxylic acid [1 -(4-aminobenzyl)-2-oxo-2-(3-oxo-b.exahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
4-Phenylthiophene-2-carboxylic acid [1 -(4-aminobenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
4-Pyridin-3-yltbiophene-2-carboxylic acid [1 -(4-aminobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
2-Thiopb.en-2-ylthiazole-4-carboxylic acid [1 -(4-aminobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]atnide
2-Methyl-5-(pyrrolidine-l-sulfonyl)furan-3-carboxylic acid [l-(4-aminoben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fc]pyrrol-4-yl)ethyl]amide
3-Phenylpyrrole-l-carboxylic acid [l-(4-aminobenzyl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-6]pytrol-4-yl)ethyl]amide
N-[l -Benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fc]pyrrol-4-yl)ethyl] benzamide
4-fe^^Butyl-A^-[l.ben2yl-2-oxo-2-(3-oxo-l^exahydrofuro[3s2-fe]pyr^ol-4-yl) ethyl]benzamide
7sT-[l-Benzyl-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-trifluoro methoxybenzamide
^-Dimethylamino-7y/'-[l-ben2yl-2-oxo-2-(3-oxo-liexahydrofuro[3,2-6]pyrrol-4-/l)ethyl]benzamide
7/-[l-Benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)et;hyl]-4-isopropyl benzamide
4-Difluoromethoxy-JVr-[l-ben2yl-2-oxo-2-(3-oxo-liexaliydrofuro[3,2-&]pyrrol-4-yl)ethyl]benzamide
A^-[l-Benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-trifluoro methylbenzamide
4-Bromo-JV-[l-benzyl-2-oxo-2-(3-oxo-hexah.ydrofuro[3,2-&]pyrrol-4-yl)ethyl] benzamide
3-Bromo-JV-[l-benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)ethyl] benzamide
N-[l-Ben2yl-2-oxo-2-(3-oxo-hexahydroforo[3,2-6]pyrrol-4-yl)ethyl]-4-vinyl benzamide
Naphthalene-2-carboxylic acid [l-ben2yl-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-6] pyirol-4-yl)ethyl]amide
Naphthalene-1 -carboxylic acid [l-benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe] pyrrol-4-yl)ethyl]amide
Quinoline-6-cafboxylic acid [l-benzyl-2-oxo-2-(3-oxo-hexah.ydrofuro[3,2-6] pyrrol-4-yl)ethyl]amide
Benzo[£]thiophene-2-carboxylic acid [l-benzyl-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]amide
Benzo[6]thiophene-3-carboxylic acid [l-benzyl-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)eihyl]amide
Benzothiazole-5-carboxylic acid [l-benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6] pyrrol-4-yl)ethyl] amide
Biphenyl-4-carboxylic acid [l-ben2yl-2-oxo-2-(3-oxo-hexaliydrofuro [3,2-6] pyrrol-4-yl)ethyl]amide
Ar-[l-Benzyl-2-oxo-2-(3-oxo-hexahydrofliro[352-6]pyiTol-4-yl)ethyl]-4-plienoxy benzamide
A'-[l-Benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3J2-fe]pyrrol-4-yl)ethyl]-3-phenoxy benzamide
JV-[l-Ben2yl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl3-4-imidazol-l-ylbenzamide
JV-[l-Ben2yl-2-oxo-2-(3-oxo-hexahydrofuro[3J2-6]pyrrol-4-yl)ethyl]-4-thiophen-2-ylbenzamide
7/-[l-Benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]-4-oxazol-5-ylbeazamide
^-[l-Benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)etliyl]-4-[l>2,3] thiadiazol-5-ylbenzamide
JV-[l-Benzyl-2-oxo-2-(3-oxo-hexahydrofiiro[332-fe]pyrrol-4-yl)etliyl]-4-pyrazol-l-ylbenzamide
AT-[l-Benzyl-2-oxo-2-(3-oxo-hexa]iydrofuro[352-&]pyrrol-4-yl)ethyl]-5-tbiophen-2-ylnicotinamide
7V-[l-Ben2yl-2-oxo-2-(3-oxo-liexaliydrofuro[3,2-&]pyrrol-4-yl)ethyl]-6-plienyl nicotinamide
2-Phenyltbiazole-4-carboxylic acid [l-benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
2-Pyridin-3-ylthiazole-4-carboxylic acid [l-benzyl-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl] amide
5-Phenylthiopb.ene-2-carboxylic acid [l-ben2yl-2-oxo-2-(3-oxo-hexahydrofuro [3,2-&]pyrrol-4-yl)ethyl]amide
5-Pyridin-3-ylthiophene-2-carboxylic acid [l-benzyl-2-oxo-2-(3-oxo-hexahydrofuro 3,2-i]pyrrol-4-yl)eihyl]ainide
2-Methyl-5-phenylfuran-3-cafboxylic acid [ 1 -benzyl-2-oxo-2-(3-oxo-hexahydro furo[3,2-6]pyrrcl-4-yl)ethyl]amide
4-Phenylthiopbene-2-carboxylic acid [l-ben2yl-2-oxo-2-(3-oxo-hexa hydrofuro [3,2-i]pyrrol-4-yl)ethyl]amide
4-Pyridin-3-ylthiophene-2-carboxylic acid [l-ben2yl-2-oxo-2-(3-oxo-hexahydro furo[3J2-^]pyrrol-4-yl)ethyl]amide
2-Thiophen-2-ylthiazole-4-carboxylic acid [ 1 -benzyl-2-oxo-2-(3-oxo-hexahydro furo[3,2-Z>]pyrrol-4-yl)ethyl]amide
2-Methyl-5-(pyrrolidine-l-sulfonyl)furan-3-carboxylic acid [l-benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
3-Phenylpyrrole-l-carboxylic acid [l-benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3}2-i]pyrrol-4-yl)ethyl]amide
4-[2-(4-fert-Butyl-benzylsulfanyl)-4-methyl-pentanoyl3-tetrahydrofuro[3,2-b3 pyrrol-3 -one
4_[2-(4-fer^-Butyl-benzylsulfanyl)-3-(4-hydroxy-phenyl)-propionyl]-tetrahydro-furo[3,2-b]pyrrol-3-one
2-Isobutyl-l-(3-oxo-hexahydrofuro[3,2-b]pyrrol-4-yl)-4-(3-phenyl-pyrrol-l-yl)-butane-l,4-dione
2-Isobutyl-l:(3-oxo-hexahydrofuro[3,2-b]pyrrol-4-yl)-4-(3-phenyl-pyrrolidin-l-yl)-butane-l,4-dione
2-(4-Hydroxyben2yl)-l-(3-oxo-hexahydrofaro[3,2-b]pyrrol-4-yl)-4-(3-phenyl-pyrrol-1 -yl)-butane-l ,4-dione
2-(4-Hydroxybenzyl)-l-(3-oxo-hexahydrofuro[3,2-b]pyrrol-4-yl)-4-(3-phenyl-pyrrolidin- 1 -yl)-butane- 1 ,4-dione
4-(l,3-Dib.ydro-isoindol-2-yl)-2-isobutyl-l-(3-oxo-hexaliydroforo[3,2-b]pyrrol-4-yl)-butane-1 ,4-dione
,3-Dihydro-isoindol-2-yl)-2-(4-hydroxybenzyl)- 1 -(3-oxo-hexahydrofuro[3 ,2-b]pyrrol-4-yl)-butane-l ,4-dione
4-(3,4-Dihydro-lH-isoquinolin-2-yl)-2-isobutyl-l-(3-oxo-hexahydrofuro[3,2-b]pyrrol-4-yl)-butane-l ,4-dione
4-(3,4-Dihydro-lH-isoquinolm-2-yl)-2-(4-hydroxybenzyl)-l-(3-oxo-hexab.ydro-furo[3,2-b]pyrrol-4-yl)-butane-ls4-dione
Further compounds of the invention include, but are not limited to, the following examples that are the (3aS, 7&R) or (3aR, 7aS) isomer of general formula (II), where Z = 'O' and R1 = 'H' and also include the equivalent analogues included in the full definition of Z and R1
Air-[l-(4-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[332-6]pyridin-4-yl)ethyl] benzamide
4-fert-Butyl-7V-[l-(4-liydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-i]pyridm-4-yl)ethyl]benzamide
A^[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydroftiro[3,2-6]pyridm-4-yl)ethyl]-4-trifluoromethoxybenzamide
4~Dimethylainino-JV'-[l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fc]pyridin-4-yl)ethyl]benzamide
7V-[l-(4-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)eth.yl]-4-isopropylbenzamide
4-Difluoromethoxy-7V-[l-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyridin-4-yl)ethyl]benzamide
77-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-&]pyridin-4-yl)ethyl]-4-trifluoromethylbenzamide
4-Bromo-JV-[l-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]benzamide
3-Bromo-^V-[l-(4-b.ydroxyben2yl)-2-oxo-2-(3-oxo-hexahydroftiro[3,2-6]pyridin-4-yl)eth.yl]benzamide
//-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]-4-vinylbenzamide
Naphthalene-2-carboxylic acid [l-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyridin-4-yl)ethyl]amide
Naphthalene-1-carboxylic acid [l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-b.exahydrofuro [3,2-fe]pyridin-4-yl)ethyl]amide
Quinoline-6-carboxylic acid [l-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyridin-4-yl)ethyl]amide
Benzo[6]thiophene-2-carboxylic acid [l-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexa hydrofuro[352-fe]pyridin-4-yl)ethyl]amide
Benzo[6]thiophene-3-carboxylic acid [l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-fe]pyridin-4-yl)ethyl]amide
Benzothiazole-5-carboxylic acid [l-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-i]pyridin-4-yl)ethyl]amide
Biphenyl-4-carboxylic acid [1 -(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-fe]pyridin-4-yl)ethyl]amide
^[l-(4-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyridin-4-yl)ethyl]-4-phenoxybenzamide
7/-[l-(4-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydroforo[3,2-^]pyridin-4-yl)ethyl]-3-phenoxyben2amide
7^-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]-4-imidazol-1 -ylbenzamide
AL[l-(4-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexab.ydrofuro[3,2-i]pyridin-4-yl)ethyl]-4-thiophen-2-ylbenzamide
oxazol-5-ylbenzamide
jV-[l-(4-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]-4-[ 1,2,3]thiadiazol-5-ylbenzamide
JV-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydroforo[3,2-6]pyridin-4-yl)eth.yl]-4-pyrazol-1 -ylbenzamide
Ar-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[352-6]pyridin-4-yl)ethyl]-5-tbiophen-2-ylnicotinamide
JV-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]-6-phenylnicotinamide
2-Phenylthiazole-4-carboxylic acid [l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-fe]pyridin-4-yl)ethyl]amide
2-Pyridin-3-ylthiazole-4-carboxylic acid [ 1 -(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyridin-4-yl)ethyl]amide
5-Phenylthiophene-2-carboxylic acid [l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-^]pyridin-4-yl)ethyl]amide
5-Pyridin-3-yltbiophene-2-carboxylic acid [l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-b.exahydrofuTo[3.)2-fc]pyridin-4-yl)ethyl]ainide
2-Methyl-5-phenylfuran-3-carboxylic acid [l-(4-b.ydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3J2-6]pyridin-4-yl)ethyl]amide
4-Phenylthiophene-2-carboxylic acid [ 1 -(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexa hydrofuro[3s2-&]pyridin-4-yl)ethyl]amide
4-Pyridin-3-ylthiophene-2-carboxylic acid [l-(4-bydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-£]pyridin-4-yl)ethyl]amide
2-Thiophen-2-ylthiazole-4-carboxylic acid [l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyridin-4-yl)ethyl]amide
2-Methyl-5-(pyrrolidine-1 -sulfonyl)fiiran-3-carboxylic acid [ 1 -(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyridin-4-yl)eihyl]amide
3-Phenylpyrrole-l-carboxylic acid [l-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-6]pyridin-4-yl)ethyl]amide
^-[3-Me1hyl-l-(3-oxo-hexahydrofuro[3J2-^]pyridine-4-carbonyl)butyl]benzainide 4-te7^Butyl-7V"-[3-methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl) butyl] benzamide
7V-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl) butyll-4-trifluoro methoxybenzamide
4-Dimethylamino-7/-[3-methyl-l-(3-oxo-hexahydrofuro[3J2-fc]pyridine-4-carbonyl) butyl]benzamide
4-Isopropyl-?/-[3-methyl-l-(3-oxo-hexaliydrofuro[3,2-&]pyridine-4-carbonyl) butyl] benzamide
4-Difluoromethoxy-?/-[3-methyl-l-(3-oxo-liexahydrofuro[3,2-6]pyridine-4-carbonyl) butyljbenzamide
Ar-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl) butyl]-4-trifluoro methylbenzamide

4-Bromo-7V-[3-methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-caxbonyl) butyl] benzamide
3-Bromo-JV-[3-metb.yl-l-(3-oxo-hexahydrofuro[352-fe]pyridine-4-carbonyl) butyl] benzamide
.V-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-fe]pyridine-4-carbonyl) butyl]-4-vinyl benzamide
Naphthalene-2-carboxylic acid [3-memyl-l-(3-oxo-hexahydrofuro[3,2-&]pyridine-4-carbonyl) butyl]amide
Naphthalene- 1 -carboxylic acid [3-methyl- 1 -(3-oxo-hexahydrofuro [3 ,2-6]pyridine~4-carbonyl) butyl]amide
Quinoline-6-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl) butyl]amide
Benzo[fe]thiophene-2-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-i] pyridine-4-carbonyl) butyl]amide
Benzo[6]tbiophene-3-carboxylic acid [3-methyl- 1 -(3-oxo-hexahydrofuro[3,2-6] pyridine-4-carbonyl) butyl]amide
Benzothiazole-5-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl) butyl] amide
Biphenyl-4-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-Z>]pyridine-4-carbonyl) butyl]amide
l-l -(3-oxo-hexahydrofuro[3 ,2-6]pyridine-4-carbonyl) butyl]-4-phenoxy benzamide
Ar-[3-Methyl-l-(3-oxo-hexahydrofbro[3,2-6]pyridine-4-carbonyl) butyl]-3-phenoxy benzamide
4-Imidazol-l-yl-AT-[3-methyl-l-(3-oxo-hexaliydrofiiro[3,2-6]pyridine-4-carbonyl) butyl]benzamide
JV-[3-Methyl-l-(3-oxo-b.exahydrofuro[3,2-&]pyridine-4-carbonyl) butyl]-4-thiophen-2-ylbenzamide
JN7-[3-Meihyl-l-(3-oxo-hexahydrofuro[352-6]pyTidine-4-carboiiyl) butyl]-4-oxazol-5-ylbenzamide
-V-t3-Methyl-l-(3-oxo-hexaliydrofuro[3,2-&]pyridine-4-carbonyl) butyl]-4-[ 1,2,3] tbiadiazol-4-ylbenzamide
JV-[3-Methyl-l-(3-oxo-hexahydrofuro[3)2-6]pyridine-4-carbonyl) butyl]-4-pyrazol-l-ylbenzamide
JV-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-cafbonyl) butyl]-5-thiopb.en-2-ylnicotinamide
JV-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-63pyridine-4-carbonyl) butyl]-6-phenyl nicotinamide
2-Phenylthiazole-4-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-6] pyridine-4-carbonyl) butyl]amide
2-Pyridin-3-ylthia2ole-4-carboxylic acid [3-methyl-l -(3-oxo-hexahydrofuro[3,2-b]pyridine-4-carbonyl) butyl]amide
5-Phenylthiophene-2-carboxylic acid [3-methyl-1 -(3-oxo-hexahydrofuro[3,2-b] pyridine-4-carbonyl) butyl]amide
5-Pyridin-3-ylthiophene-2-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-&] pyridine-4-carbonyl) butyl] amide
2-Methyl-5-phenylfuran-3-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-&] pyridine-4-carbonyl) butyljamide
4-Phenylthiophene-2-carboxylic acid [3-methyl-1 -(3-oxo-hexahydrofuro [3,2-b] pyridine-4-carbonyl) butyl]amide
4-Pyridin-3-ylthiophene-2-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-6j pyridine-4-carbonyl) butyljamide
2-Trhiophen-2-ylthiazole-4-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-&] pyridine-4-carbonyl) butyl]amide
2-Methyl-5-(pyrrolidine-l-sulfonyl)furan-3-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-&] pyridine-4-carbonyl) butyl]amide
3-Phenylpyrrole-l-carboxylic acid [3-methyl-l -(3-oxo-hexahydrofuro[3,2-6] pyridine-4-carbonyl) buryljamide
Additional compounds of the invention include, but are not limited to, the following examples that are the (3aS, 6a#) isomer of general formula (I), where Z = 'O' and R1 = CH', and also include the equivalent analogues included in the full definition of Z andR1
N~ [ 1 -Cyclopropyhnethyl-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]benzamide
4-fe7t-Butyl-JV-[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexaiiydrofuro[3J2-6]pyrrol-4-yl)ethyl]benzamide
Ar-[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-trifluoromethoxybenzamide
4-Dimethylamino-7V'-[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexa]iydrofuro[3,2-6]pyrrol-4-yl)etb.yl]benzamide
4-Isopropyl-A7-[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide
4-Difluoromethoxy-7>/-[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-b.exahydrofuro[3,2-fc]pyrrol-4-yl)ethyl]benzamide
A7-[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)ethyl]-4-trifluoroinethylbenzainide
4-Bromo-7V"-[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-^]pyrrol-4-yl)ethyl]benzamide
3-Bromo-A7:-[l-Cyclopropylinethyl-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide
A-[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydroftiro[3J2-6]pyrrol-4-yl)ethyl]-4-vinylbenzamide
Kapb.thalene-2-carboxylic acid [l-Cyclopropylmethyl-2-oxo-2>-(3-oxo-
hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
Naphthalene-1-carboxylic acid [l-Cyclopropylmethyl-2-oxo-2-(3-oxo-
hexahydrofuro[3,2-fe]pyrrol-4-yl)ethyl]ainide
Quinoline-6-carboxylic acid [l-Cyclopropylmethyl-2-oxo-2-(3-oxo-
hexab.ydrofuro[3,2-&]pyrrol-4-yl)ethyl]amide
Benzo[fe]thiophene-2-carboxylic acid [l-Cyclopropylmeihyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]ainide
Benzo[6]thiophene-3-carboxylic acid [l-Cyclopropybnethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
Benzothiazole-5-carboxylic acid [l-Cyclopropylmethyl-2-oxo-2-(3-oxo-
hexab.ydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
Biphenyl-4-carboxylic acid [l-Cyclopropylmethyl-2-oxo-2-(3-oxo-
hexab.ydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
JV-[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3J2-i]pyrrol-4-yl)ethyl3-4-phenoxy benzamide
JV-[l-Cyclopropylmethyl-2--oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)e1:hyl]-3-phenoxy benzamide
4-Imidazol-l -yl-N-[ 1 -Cyclopropylmethyl-2-oxo-2-(3 -oxo-hexahydrofiiro[3,2-6]pyrrol-4-yl)ethyl] benzamide
JV"-[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-thiophen-2-ylbenzamide
JV-[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-^]pyrrol-4-yl)ethyl]-4-oxazol-5-ylbenzamide
^[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexah.ydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-[1,2,3] thiadiazol-4-ylbenzatnide
JV-[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-4-pyrazol-1 -ylbenzamide
?/-[l-Cyclopropylinethyl-2-oxo-2-(3-oxo-liexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-5-thiophen-2-ylnicotinamide
2V-[l-Cyclopropylmethyl-2-oxo-2-(3-oxo-liexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]-6-phenyl nicotinamide
2-Phenylthiazole-4-carboxylic acid [l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofiiro[3,2-£]pyrrol-4-yl)ethyl]amide
2-Pyridin-3-ylthiazole-4-carboxylic acid [l-Cyclopropylmeihyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
5-Phenylthiophene-2-carboxylic acid [l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)ethyl]ainide
5-Pyridin-3-ylthiophene-2-carboxylic acid [1 -Cyclopropylmethyl-2-oxo-2-(3-oxo-b.exaliydrofuro[3,2-b] pyrrol-4-yl)ethyl]amide
2-Methyl-5-phenylfuran-3-carboxylic acid [1 -Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-Z»]pyrrol-4-yl)ethyl]amide
4-Phenylthiophene-2-carboxylic acid [l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
c acid [l-Cyclopropylrnethyl-2-oxo-2-(3-oxo-hexahydrofuro[352-6]pyrrol-4-yl)ethyl]amide
2-TMophen-2-ylthiazole-4-carboxylic acid [l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
2-Methyl-5-(pyrrolidine-l -sulfonyl)furan-3-carboxylic acid [1 -Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
3-Phenylpyrrole-l-carboxylic acid [l-Cyclopropylmethyl-2-oxo-2-(3-oxo-hexahydrofiaro[3,2-i]pyrrol-4-yl)ethyl]amide
Ar-[l-(3-Oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl)butyl]benzamide 4-tert-'B\tfy\-N-[ 1 -(3-oxo-hexahydrofuro [3,2-6]pyrrole-4-carbonyl) butyl] benzamide
N-[l -(3-Oxo-hexahydroforo[3,2-6]pyrrole-4-carbonyl) butyl]-4-trifluoromethoxy benzamide
4-Dimethylanimo-JV'-[l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]benzamide
4-Isopropyl-A^[l-(3-oxo-hexahydroruro[3,2-6]pyrrole-4-carbonyl) butyl] benzamide
4-Difluoromethoxy-7V-[ 1 -(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]benzamide
/f-[l-(3-Oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]-4-trifluoromethyl benzamide
4-Bromo-JV-[l -(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl] benzamide
i
3-Bromo-Ar-[l -(3-oxo-hexahydrof
N-[l -(3-Oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]-4-vinyl benzamide
Naphthalene-2-carboxylic acid [1 -(3-oxo4iexahydrofaro[3,2-6]pyrrole-4-carbonyl) butyl]amide
Naphthalene- 1-carboxylic acid [l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]amide
Quinoline-6-carboxylic acid [1 -(3-oxo-hexahydrofuro[3,2-fc]pyrrole-4-carbonyl) butyl]amide
Benzo[fe]tbiophene-2-carboxylic acid [l-(3-oxo-hexahydrofuro[3,2-&]pyrrole-4-carbonyl) butyljamide
Benzo[6]tbiophene-3-carboxylic acid [ 1 -(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]amide
Benzothiazole-5-carboxylic acid [l-(3-oxo-hexahydrofuro[3,2-i]pyrrole-4-carbonyl) butyl]amide
Biphenyl-4-carboxylic acid [l-(3-oxo-nexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]amide
JV-[l-(3-Oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl)butyl]-4-phenoxy benzamide 7V-[l-(3-Oxo-hexahydrofuro[3,2-fe]pyrrole-4-carbonyl)butyl]-3-phenoxy benzamide
4-Imidazol-l-yl-]V-[l-(3-oxo-liexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]
benzamide
7V-[l-(3-Oxo-hexahydroforo[3,2-6]pyrrole-4-carbonyl) butyl]-4-thiophen-2-y
Ibenzamide
AT-[l-(3-Oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]-4-oxazol-5-yl
benzamide
Ar.[l-(3-Oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]-4-[l,2,3]thiadiazol-4-yl benzamide
N-[l-(3-Oxo-hexahydrofuro[3,2-&]pyrrole-4-carbonyl) buty!]-4-pyrazol-l-yl
benzamide
JV-[l-(3-Oxo-hexahydrofiiro[3,2-6]pyrrole-4-cafbonyl) butyl]~5-thiophen-2-yl
nicotinamide
A^[l-(3-Oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl)butyl]-6-phenylmcotinamide
2-Phenylihiazole-4-carboxylic acid [l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-cafbonyl) butyl]amide
2-Pyridin-3-yltbiazole-4-carboxylic acid [1 -(3-oxo-hexah.ydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]amide
5-Phenylthiophene-2-cafboxylic acid [l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl] amide
5-Pyridin-3-yltbiophene-2-carboxylic acid [l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl] amide
2-Methyl-5-phenylfuran-3-carboxylic acid [1 -(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl] amide
4-Phenyltbiophene-2-carboxylic acid [1 -(3-oxo-hexahydrofuro[3,2-i]pyrrole-4-cafbonyl) butyl] amide
4-Pyridin-3-ylthiophene-2-carboxylic acid [l-(3-oxo-hexahydrofuro[3,2-fe]pyrrole-4-carbonyl) butyl] amide
2-Thiophen-2-ylthiazole-4-carboxylic acid [l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyljamide
2-Methyl-5-(pyrrolidme-l-sulfonyl)furan-3-carboxylic acid [l-(3-oxo-hexahydro furo[3,2-Z>]pyirole-4-carbonyl)butyl]amide
3-Phenylpyrrole-l-carboxylic acid [l-(3-oxo-hexahydrofuro[3,2-6]pyrrole -4-carbonyl) butyl]amide
4-Memyl-JV-[3-methyl-l-(3-oxo-hexahydrofuro[3,2-b]pyrrole-4-carbonyl) butyl] ben2amide
4-Methoxy-JV'-[3-methyl-l-(3-oxo-hexahydrofuro[3,2-b]pyrrole-4-carbonyl) butyl] benzamide
JV-[3-Memyl-l-(3-oxo-hexahydrofuro[3,2-b]pyrrole-4-carbonyl)butyl]-4-morpholin-4-ylbenzamide
4-fe^Butyl-A^[l-(4-methoxybenzyl)-2-oxo-2-(3-oxo-hexab.ydrofUro[3J2-i]pyrrol-4-yl)ethyl]benzamide
Naphthalene-2-carboxylic acid [ 1 -(4-methoxybenzyl)-2-oxo-2-(3 -oxo-hexahydro furo[3,2-b]pyrrol-4-yl)ethyl]amide
Biphenyl-4-cafboxylic acid [l-(4-methoxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-fe]pyrrol-4-yl)ethyl] amide
4-te/^-Butyl-//-[l-(4-liydroxyniethylbenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide
Naphthalene-2-carboxylic acid [l-(4-hydroxymethylbenzyl)-2-oxo-2-(3-oxo-hexahydrofaro[3,2-£]pyrrol-4-yl)ethyl]amide
Biphenyl-4-carboxylic acid [l-(4-hydroxymethylbenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
Furan-2-carboxylic acid [1 -(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-Z?]pyrrol-4-yl)ethyl] amide
Furan-3-carboxylic acid [l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl] amide
Thiophene-2-carboxylicacid[l-(4-liydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]amide
Tbiophene-3-carboxylicacid[l-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]amide
Morpholine-4-carboxylic acid [ 1 -(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-fc]pyrrol-4-yl)ethyl]amide
^V'-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-2-phenylacetamide
JV-[l-(4-Hydroxyben2yl)-2-oxo-2-(3-oxo-liexaliydrofuro[3,2-&]pyrrol-4-yl)ethyl]-3-phenylpropionamide
Furan-2-cafboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-fc]pyrrole-4-cafbonyl) butyl]amide
Furan-3-carboxylic acid [3-methyl-l-(3-oxo-hexahydrofuro[3,2-i]pyrrole-4-carbonyl) butyl] amide
Thiophene-2-carboxylicacid[3-methyl-l-(3-oxo-hexahydrofuro[3,2-^]pyrrole-4-carbonyl) butyl]amide
Thiophene-3-carboxylic acid [3-methyl-l-(3-oxo-hexahydroiuro[3,2-fe]pyrrole-4-cafbonyl) butyl]amide
Morpholine-4-carboxylicacid[3-methyl-l-(3-oxo-b.exahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]amide
^-[3-Methyl-l-(3-oxo-hexahy6^ofuro[3,2-fe]pyrrole-4-carbonyl)butyl]-2-phenyl acetamide
/^-[S-Methyl-l-CS-oxo-hexahydrofurotS^-ijpyrrole^-carbonyObutyll-S-phenyl propionamide
Furan-2-carboxylic acid [1 -cyclohexylmethyl-2-oxo-(3-oxo-hexahydrofuro[3,2-b] pyrrol-4-yl)ethyl]amide
Furan-3-carboxylic acid [l-cyclohexylmethyl-2-oxo-(3-oxo-hexahydroruro[3,2-6]pyrrol-4-yl)ethyl]amide
Thiophene-2-carboxylic acid [l-cyclohexyhnethyl-2-oxo-(3-oxo-hexahydro furo[3,2-6]pyrrol-4-yl)ethyl]amide
Thiophene-3-carboxylic acid [l-cyclohexylmethyl-2-oxo-(3-oxo-hexahydro furo[3,2-6]pyrrol-4-yl)ethyl] amide
Morpholine-4-carboxylicacid[l-cyclohexylmethyl-2-oxo-(3-oxo-hexahydro foro[3,2-£]pyrrol-4-yl)ethyl]amide
A^-[l-Cyclohexylmethyl-2-oxo-(3-oxo-liexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-2-phenyl acetamide
N-[l-Cyclohexylmetiiyl-2-oxo-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-3-phenylpropionamide
Ar-[l-Cyclohexylmethyl-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl] benzamide
Naphthalene-1 -carboxylic acid [ 1 -cy clohexylmethyl-2-oxo-2-(3 -oxo-hexahydro furo[3,2-3]pyrrol-4-yl)ethyl]amide
Benzo[6]thiophene-2-cafboxylic acid [1 -cyclohexyhnethyl-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
Furan-2-carboxylicacid[3,3-dimethyl-l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]amide
Furan-3-carboxylicacid[3,3-dimethyl-l-(3-oxo-hexahydrofuro[352-63pyrrole-4-carbonyl) butyl]amide
Thiophene-2-carboxylic acid [3,3 -dimethyl-1 -(3-oxo-hexahydrofuro [3,2-6]pyrrole-4-carbonyl) butyl]amide
Thiophene-3-carboxylic acid [3,3-dimethyl-l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl] amide
Morpholine-4-carboxylic acid [3,3-dimethyl-l -(3-oxo-hexahydrofuro[3,2-i]pyrrole-4-carbonyl) butyljamide
Ar-[3^-Dimethyl-l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl)butyl]-2-plienyl acetamide
JV-[3,3-Dimethyl-l-(3-oxo-hexaliydrofuro[3,2-6]pyrrole-4-carbonyl)butyl]-3-p1ienyl propionamide
Benzo[6]thiophene-2-carboxylicacid[3,3-dimethyl-l-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyljamide
JV-[33-Dimethyl-l-(3-oxo-hexaliydrofuro[3,2-Z>]pyrrole-4-carbonyl) butyl] benzamide
Furan-2-carboxyHcacid[l-ben27l-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl) ethyl]amide
Furan-3-carboxylicacid[l-ben2yl-2-oxo-2-(3-oxo-hexab.ydrofuro[3,2-6]pyrrol-4-yl) ethyl]amide
Thiophene-2-carboxylicacid[l-ben2yl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-£>]pyrroi-4-yl)ethyl]amide
Thiophene-3-carboxylic acid [ 1 -benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)ethyl] amide
Morpholine-4-carboxylic acid [1 -benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&] pyrroI-4-yl)ethyl]amide
JV-[l-Benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)eihyl]-2-plienyl acetamide
propionamide
Benzo[6]tliiophene-2-carboxylicacid[l-benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]amide
A^-[l-Ben2yl-2-oxo-2-(3-oxo-hexahydrof\ro[3,2-6]pyrrol-4-yl)ethyl]ben2amide
Furan-2-carboxylicacid[l-cyclopentylmethyl-2-oxo-(3-oxo-liexahydrofuro[3,2-6] pyrrol-4-yl)ethyl]amide
Furan-3-cafboxylic acid [l-cyclopentylmethyl-2-oxo-(3-oxo-hexahydro furo[3,2-&]pyrrol-4-yl)ethyl]amide
Thiophene-2-carboxylic acid [l-cyclopentylmethyl-2-oxo-(3-oxo-hexahydro furo[3,2-i]pyrrol-4-yl)ethyl]amide
Thiophene-3-carboxylic acid [l-cyclopentylmethyl-2-oxo-(3-oxo-hexahydro furo[3,2-i]pyrrol-4-yl)ethyl]amide
Morpholine-4-carboxylic acid [l-cyclopentylmethyl-2-oxo-(3-oxo-hexahydro furo[3,2-&]pyrrol-4-yl)ethyl]amide
A^-[l-Cyclopentylmeihyl-2-oxo-(3-oxo-hexahydrofuro[3,2-fo]pyrrol-4-yl)ethyl]-2-phenylacetamide
A?-[l-Cyclopentylinethyl-2-oxo-(3-oxo-hexaliydrofuro[3,2-6]pyrrol-4-yl)ethyl]-3-phenylpropionamide
N-[l -Cyclopentylmethyl-2-oxo-2-(3-oxo-hexahydroforo [3,2-6]pyrrol-4-yl)ethyl] benzamide
Naphthalene-1-carboxylic acid [l-cyclopentylmetliyl-2-oxo-2-(3-oxo-liexahydro furo[3 ,2-&]pyrrol-4-yl)ethyl]atnide
Benzo[6]thiophene-2-carboxylic acid [l-cyclopentylmethyl-2-oxo-2-(3-oxo-hexa
Furan-2-carboxylic acid [l-benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexaliydro furo[3 ,2-6]pyrrol-4-yl)ethyl]amide
Furan-3-carboxylic acid [l-benzylsulfanyLmethyl-2-oxo-2-(3-oxo-hexahydro furo[332-fe]pyrrol-4-yl)ethyl]amide
Thiophene-2-carboxylic acid [l-benzylsulfanylmethyl-2-oxo-2-(3-oxo-liexahydro furo[3,2-fe]pyrrol-4-yl)ethyl]aimde
Tbiophene-3-carboxylic acid [l-benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexaliydro furo[3,2-fc]pyrrol-4-yl)ethyl]amide
Morpholine-4-carboxylic acid [1 -ben2ylsulfanylmethyl-2-oxo-2-(3-oxo-liexahydro furo[3 ,2-&]pyrrol-4-yl)ethyl]amide
Ar-[l-Beiizylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydrofurot3,2-63pyrrol-4-yl)ethyl3-2-phenylacetamide
A^-[l-Benzylsulfanylme1iiyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-3-pb.enylpropionamide
Benzo[fe]thiophene-2-carboxylic acid [1 -ben2ylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]ainide
7^-[l-Benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexah.ydrofuro[3,2-6]pyrrol-4-yl) ethyl]benzamide
Naphthalene-1 -carboxylic acid [l-ben2ylsulfanylmethyl-2-oxo-2~(3-oxo4iexahydro foro[3,2-6]pyrrol-4-yl)ethyl]amide
Furan-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l -(phenylmethanesulfonylmethyl)ethyl]amide
Furan-3-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-(phenylmethanesulfonylmetliyl)eihyl]ainide
Thiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-1 -(phenylmethanesulfonylmethyl)ethyl]amide
Thiophene-3-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-l-(phenylmethanesulfonylmethyl)ethyl]amide
Morpholine-4-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-1 -(phenylmethanesulfonylmethyl)eihyl]amide
JV-[2-Oxo-2-(3-oxo-liexahydrofuro[3,2-6]pyrrol-4-yl)-l-(phenylmethanesulfonyl methyl)ethyl]-2-phenylacetamide
7V-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-i]pyrrol-4-yl)-l-(phenylmethanesulfonyl methyl)ethyl]-3-phenylpropionamide
Benzo[6]thiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3)2-6]pyrrol-4-yl)-l-(phenylmethanesulfonylmethyl)ethyl]ainide
JV-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)-1 -(phenylmethanesulfonyl methyl)ethyl]benzamide
Naphthalene-1-carboxylic acid [2-oxo-2-(3-oxo-b.exahydrofuro[3,2-6]pyrrol-4-yl)-l-(phenylmethanesulfonylmethyl)ethyl]ainide
4-(2-Benzyloxy-3-cyclohexyl-propionoyl)tetrahydrofuro[352-6]pyrrol-3-one 4-[2-(4-terr-Butylbenzyloxy)-4-methyl-pentanoyl]tetrahydrofuro[3,2-b]pyrrol-3-one
4-[2-(Naphthalen-l-ylmethoxy)-3-phenyl-propionyl]-tetrahydro-furo[3,2-b]pyrroi-3-one
4-[3-Cyclohexyl-2-(foran-2-ylmethanesulfanyl)propionoyl]tetrah.ydrofuro[3j2-b]pyrrol-3-one
4-[3-Cyclohexyl-2-(furan-3-ybnethanesulfanyl)propionoyl]tetrahydrofiiro[3,2-b]pyrrol-3-one
4-[3-C^clohexyl-2-(furan-2-ylmetbanesulphonyl)propionoyl]tetrahydrofuro[3 b]pyrrol-3-one
4-[3-C^clohexyl-2-(furan-3-ylmethanesulphonyl)propionoyl]tetrab.ydrofuro[3,2-b]pyrrol-3-one
Morpholine-4-carboxylic acid 1 -cyclohexylmethyl-2-oxo-2-(3-oxo-hexahydro-furo [3,2-b]pyrrol-4-yl)-ethyl ester
Morpb.oline-4-cafboxylic acid 3-methyl-l-(3-oxo-b.exahydrofuro[3,2-b]pyrrole-4-carbonyl)-butyl ester
Morpholine-4-cafboxylic acid 3,3-dimemyl4-(3-oxo-hexahydrofuro[3,2-b] pyrrole-4-carbonyl)-butyl ester
2-Cyclohexylmethyl-4-morpholin-4-yl-l-(3-oxo-hexaliydroftiro[3,2--b3pyrrol-4-yl)butane-l ,4-dione
2-Isobutyl-4-morpholin-4-yl-l-(3-oxo-hexahydrofuro[3,2-b]pyrrol-4-yl)-butane-l,4-dione
2-(2,2-Dimethyl-propyl)-4-morpholin-4-yl-l-(3-oxo-hexahydrofuro[352-b]pyrrol-4-yl)-butane-l ,4-dione
4-(2-Biphenyl-3-yl-4-methylpentanoyl)tetrab.ydrofuro[3,2-b]pyrrol-3-one
Further additional compounds of the invention include, but are not limited to, the following examples that are the (3aS, 7aR) or (3afl, 7aS) isomer of general formula (II) where Z = 'O' and R1 = 'H', and also include the equivalent analogues included in the full definition of Z and R1
Furan-2-carboxylicacid[l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3>2-&]pyridin-4-yl)ethyl]amide
Furan-3-cafboxylicacid[l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]amide
Thiophene-2-carboxylic acid [ 1 -(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-&]pyridin-4-yl)ethyl]amide
Thiophene-3-carboxylicacid[l-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3)2-i]pyridin-4-yl)ethyl]amide
Morpholine-4-carboxylic acid [ 1 -(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-i]pyridin-4-yl)et:hyl]amide
j\-.[ 1 -(4-Hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]-2-phenylacetamide
^•.[l_(4_Hydroxybenzyl)-2-oxo-2-(3-oxo-liexahydrofuro[3,2-6]pyridin-4-yl)ethyl]-3-phenylpropionamide
A"-[l-(4-Hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl] benzamide
Fnran-2-carboxylicacid[3-methyl-l-(3-oxo-hexahydrofuro[3,2-fr]pyridine-4-cafbonyl) butyl]amide
Furan-3-carboxylic acid [3-meth.yl-l-(3-oxo-h.exahydrofuro[3,2-6]pyridine-4-carbonyl) butyl]amide
TMophene-2-c^boxylicadd[3-methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl) butyl]amide
Thiophene-3-carboxylic acid [3-methyl-l -(3-oxo-hexahydrofuro[3,2-fc]pyridine-4-cafbonyl) butyl]amide
Morpholine-4-carboxylicacid[3-methyl-l-(3-oxo-liexaliydrofuro[3,2-6]pyridine-4-carbonyl) butyl]amide
A7-[3-Methyl-l-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl)butyl]-2-phenyl acetamide
JV>[3-Methyl-l-(3-oxo-hexah.ydrofuro[3,2-6]pyridine-4-carbonyl)butyl]-3-plienyl propionamide
Furan-2-carboxylicacid[l-cyclohexylmetliyl-2-oxo-(3-oxo-hexaliydrofuro[3,2-fe] pyridin-4-yl)eihyl]amide
Furan-3-carboxylicacid[l-cycloliexylmethyl-2-oxo-(3-oxo-hexahydroftiro[3,2-6]pyridin-4-yl)ethyl]amide
Thiophene-2-cafboxylic acid [l-cyclohexylmethyl-2-oxo-(3-oxo-hexahydro furo[3,2-6]pyridin-4-yl)ethyl]amide
Thiophene-3-carboxylic acid [l-cyclohexyhnethyl-2-oxo-(3-oxo-hexahydro faro[3,2-&]pyridin-4-yl)ethyl]amide
Morpholine-4-carboxylic acid [l-cyclohexylmethyl-2-oxo-(3-oxo-hexahydro furo[3,2-fc]pyridin-4-yl)ethyl]amide
JV"-[l-Cyclohexylmethyl-2-oxo-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]-2-phenylacetamide
7/-[l-Cyclohexyhiiethyl-2-oxo-(3-oxo-hexahydrofuro[3,2-6]pyridm-4-yl)ethyl]-3-phenylpropionamide
N-[ 1 -Cyclohexyhnethyl-2-oxo-2-(3-oxo-hexahydrofuro [3,2-ib]pyridin-4-yl)ethyl] benzamide
Naphthalene-1-carboxylic acid [l-cyclohexylmethyl-2-oxo-2-(3-oxo-hexahydro furo[3,2-fo]pyridin-4-yl)ethyl]amide
Benzo[6]thiophene-2-carboxylic acid [1 -cyclohexylmethyl-2-oxo-2-(3-oxo-hexa hydrofuro[3}2-&]pyridin-4-yl)ethyl]amide
Furan-2-carboxylicacid[3,3-dimethyl-l-(3-oxo-hexahydroforo[3,2-&]pyridine-4-carbonyl) butyl]amide
Furan-3-carboxylicacid[3,3-dimethyl-l-(3-oxo-hexahydrofuro[3,2-&]pyridine-4-carbonyl) butyl]amide
Thiophene-2-carboxylic acid [3 ,3 -dimethyl- 1 -(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl) butyl]amide
Tbiophene-3-carboxylicacid[3,3-diiiiethyl-l-(3-oxo-hexahydrofuro[3,2-63pyridine-4-carbonyl) butyl] amide
Morpholine-4-carboxylicacid[33-dimethyl-l-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl) butyl] amide
7/-[33-Dimetiiyl-l-(3-oxo-hexahydrofuro[3,2-fe]pyridine-4-carbonyl)butyl]-2-phenyi acetamide
?/-[33-Dimethyl-l-(3-oxo-hexahydrofuro[3,2-fe]pyridiiie-4-carbon.yl)butyl]-3-phenyl propionamide
Benzo[6]thiophene-2-carboxylicacid[3,3-dimethyl-l-(3-oxo-liexahydrofuro[3,2-
utyl]amide
7V-[3,3-Dimethyl-l-(3-oxo-hexahydrofuro[3,2-i>]pyridine-4-carbonyl) butyl] benzamide
Furan-2-carboxylic acid [ 1 -ben2yl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyridin-4-yl) etbyl]amide
Furan-3-carboxylicacid[l-benzyl-2-oxo-2-(3-oxo-liexahydrofaro[3,2-fe]pyridin-4-yl) ethyl] amide
Thiophene-2-carboxylic acid [l-ben2yl-2-oxo-2-(3-oxo-hexahydrofuxo[3,2-t]pyridin-4-yl)ethyl] amide
Tbiophene-3-cafboxylicacid[l-ben2yl-2-oxo-2-(3-oxo-liexahydrofuro[3s2-6]pyrrol-4-yl)ethyl]amide
Morpholine-4-carboxylic acid [1 -benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&] pyridin-4-yl)ethyl]amide
AT-[l-Ben2yl-2-oxo-2-(3-oxo-liexahydrofuro[3,2-ib]pyridin-4-yl)ethyl]-2-phenyl acetamide
//-[l-Ben2yl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-£)]pyridm-4-yl)ethyl]-3-phenyl propionamide
Benzo[fc]thiophene-2-carboxylic acid [1 -ben2yl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]amide
7f-[l-ben2yl-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-fc]pyridin-4-yl)ethyl]benz
Furan-2-carboxylicacid[l-cyclopentylmeihyl-2-oxo-(3-oxo-hexab.ydrofuro[3j2-£»] pyridin-4-yl)ethyl]amide
Furan-3-carboxylic acid [l-cyclopeaitylme1;hyl-2-oxo-(3-oxo-b.exahydro turo[3,2-fe]pyridin-4-yl)ethyl]amide
Thiophene-2-carboxylic acid [l-cyclopentylmethyl-2-oxo-(3-oxo-hexahydro furo[3,2-&]pyridin-4-yl)eihyl]amide
Thiophene-3-carboxylicacid[l-cyclopentyhiiethyl-2-oxo-(3-oxo-hexahydro furo[3,2-6]pyridin-4-yl)ethyl]amide
Morpholine-4-carboxylicacid[l-cyclopentylmethyl-2-oxo-(3-oxo-hexahydro furo[3 52-&]pyridm-4-yl)ethyl]amide
^-[l-Cyclopentylmethyl-2-oxo-(3-oxo-hexahydrofuro[3,2-Z>]pyridin-4-yl)ethyl]-2-phenylacetamide
JV-[l-Cyclopentylmethyl-2-oxo-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]-3-phenylpropionamide
JV-[l-Cyclopentylmethyl-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyridin-4-yl)ethyl] benzamide
Naphthalene- 1 -carboxylic acid [1 -cyclopentylmethyl-2-oxo-2-(3-oxo-hexahydro furo[3,2-6]pyridin-4-yl)eihyl]amide
Benzo[6]ihiophene-2-carboxylic acid [1 -cyclopentylmethyl-2-oxo-2-(3-oxo-hexa hydrofiiro[3 ,2-6]pyridin-4-yl)ethyl]amide
F\iran-2-carboxylic acid [l-benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydro furo[3,2-^]pyridin-4-yl)ethyl]amide
Furan-3-carboxylic acid [l-benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydro
Thiophene-2-carboxylic acid [1 -benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydro furo[3,2-6]pyridin-4-yl)ethyl]amide
Thiophene-3-carboxylic acid [l-benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydra furo[3,2-6]pyridin-4-yl)ethyl]amide
Morpholine-4-carboxylic acid [l-ben2ylsxdfanylmethyl-2-oxo-2-(3-oxo-hexahydro fiaro[3,2-i]pyridin-4-yl)ethyl]amide
N-[ 1 -Benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-i]pyridin-4-yl)ethyl]-2-phenylacetamide
N-[ 1 -Ben2ylsulfanylmetliyl-2-oxo-2-(3-oxo-hexaliydrofuro[3,2-6]pyridin-4-yl)ethyl]-3-plienylpropionainide
Benzo[fc]thiophene-2-carboxylic acid [1 -benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]ainide
JV"-[l-Benzylsulfanylmeihyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-Z)]pyridin-4-yl) ethyljbenzamide
Naphthalene-1-carboxylic acid [l-benzylsulfanybnethyl-2-oxo-2-(3-oxo-hexahydro furo[3,2-i]pyridin-4-yl)ethyl]amide
Furan-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofijro[3,2-fe]pyridin-4-yl)-l-(phenyhnethanesulfonylmethyl)ethyl]amide
Furan-3-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)-l-(phenykQethanesulfonybnethyl)ethyl]amide
Thiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)-l-(phenylmetiianesiilfonyhnethyl)ethyl]amide
Thiophene-3-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyridin-4-yl)-l-(phenylmethanesulfonyhnethyl)etiiyl]ainide
Morpholiiie-4-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)-l-(phenyhnethanesulfonyhnethyl)ethyl]ainide
7V-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)-l-(phenylmethanesulfonyl methyl)ethyl]-2-phenylacetamide
Ar-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyridin-4-yl)-l-(plienylinethanesulfonyl methyl)ethyl]-3-phenylpropionamide
Benzo[6]thiophene-2-carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)-1 -(phenylmethanesulfonylmethyl)ethyl]amide
7V-[2-Oxo-2-(3-oxo-hexahydrofuro[3,2-fc]pyridin-4-yl)-l-(phenylmethanesulfonyl inethyl)ethyl]benzamide
Naphthalene-1 -carboxylic acid [2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)-1 -(phenyhnethanesulfonyhnethyl)ethyl]amide
MorphoUne-4-carboxylic acid l-cyclohexylmethyl-2-oxo-2-(3-oxo-
hexahydrofuro[3,2-fc]pyridin-4-yl)ethyl ester
4-[3-Cyclohexyl-2-(furan-2-ybiethanesulfanyl)propionyl]hexahydrofuro[3J2-b] pyridin-3-one
4-[3-Cyclohexyl-2-(furan-2-yhnethanesulphonyl)propionyl]hexahydrofuro[3,2-b]pyridin-3-one
4-[3-Cyclohexyl-2-(furan-3-ylmethanesulfanyl)propionyl]hexahydrofuro[3s2-b] pyridin-3-one
4-[3-Cyclohexyl-2-(furan-3-ybnethanesulphonyl)propionyl]hexahydrofuro[3,2-b]pyridin-3-one
2-Cyclohexylmethyl-4-morpholin-4-yl-l-(3-oxo-hexahydrofuro[3,2-b]pyridm-4-yl)butane-l ,4-dione
Considering all of the above examples, it is also intended to include the oxidised analogues of capping groups that contain a readily oxidised nitrogen to give the N-oxide or a readily oxidised sulphur to give the sulphone. The following structures are illustrative examples;To those skilled in the practices of organic chemistry, compounds of general formulae (I) and (II) may be readily synthesised by a number of chemical strategies, performed either in solution or on the solid phase (see Atherton, E. and Sheppard, R. C. In 'Solid Phase Peptide Synthesis: A Practical Approach', Oxford University Press, Oxford, U.K. 1989, for a general review of solid phase synthesis principles). The solid phase strategy is attractive in being able to generate many thousands of analogues, typically on a 5-100mg scale, through established parallel synthesis methodologies (e.g. see (a) Bastos, M.; Maeji, N. J.; Abeles, R. H. Proc. Natl Acad. Sci. USA, 92,6738-6742,1995).
Therefore, one strategy for the synthesis of compounds of general formulae (I) and (El) comprises:-
(a) Preparation of an appropriately functionalised and protected bi-cyclic ketone
building block in solution.
(b) Attachment of the building block (a) to the solid phase through a linker that is
stable to the conditions of synthesis, but readily labile to cleavage at the end of a
synthesis (see James, I. W., Tetrahedron, 55(Report N9- 489), 4855-4946, 1999,
for examples of the 'linker' function as applied to solid phase synthesis).
(c) Solid phase organic chemistry (see Brown, R. D. /. Chem. Soc., Perkin Trans.1,
19, 3293-3320,1998), to construct the remainder of the molecule.
(d) Compound cleavage from the solid phase into solution.
(e) Cleavage work-up and compound analysis.
The first stage in a synthesis of compounds of general formulae (I) and (II) is the preparation in solution of a functionalised and protected building block. A typical scheme towards the tetrahydrofuro[3^-6]pyrrol-3-one (19) is detailed in Scheme 1. The following descriptions detailed in Schemes 1-13 could equally be applied using alternative scaffolds of general formulae (I) and (II).

Scheme 1. (a) 'BuOCOCl, NMM, DCM, -15°C, lOmins, under argon, (b) Diazomethane in diethyl ether, -15°C to RT over Ihr. (c) Acetic acid (d) LiCl (lOeq) in 80%aq acetic acid, 5°C to RT over Ihr.
FmOC(O) denotes the well known amine protecting group 9-fluorenyl methoxycarbonyl (Fmoc, see Atherton, E. and Sheppard, R. C., 1989) and Tg' denotes either a free hydroxyl or an hydroxyl protecting group such as tertJoutyl ether. In the illustrated case, condensation with diazomethane provides R1 = H. Although formation of the diazoketone is clearly observed at Ihr reaction, an overall improvement in isolated yield is obtained by leaving the reaction with ethereal diazomethane for 24hr.
Considering step (a), synthesis may commence from suitably protected p-hydroxyproline (17) (or protected (3-hydroxypipecolic acid), which are accessible through a variety of literature methods e.g. (a) Heffher, R. J., et al, J. Am. Chem. Soc., 114, 10181-10189, 1992; (b) Hughes, P. Cardy, J., /. Org. Chem, 54, 3260-3264, 1989, (c) Hefmer, R. J, Jouille, M. M., Tet. Lett, 30, 7021-7024, 1989, (d) Ewing, W. R., Jouille, M. M., Heterocydes, 27, 2843-2850, 1988. (e) Kolodziej, S. A., Marshall, G. R., Int. J. Pept. Prot. Res., 48, 274-280, 1996, (f) Evans, D. A., Weber, A, E., J. Am. Chem. Soc, 109. 7151-7157, 1987, (g) Langlois, N., Rakotondradany, F., Tetmhedron, 56, 2437-2448, 2000, (h) Sugisaki, C. H., et al, Tet. Lett., 39, 3413-3416, 1998, (i) Greek, C., et al, Tet. Lett, 37, 2031-, 1996, (j) Agami, C., et al, Tet. Lett., 37, 4001 -, 1996.
Activation of the suitably protected p-hydroxyproline (17) via isobutyl chloroformate mixed anhydride, followed by condensation with diazomethane, yields the diazomethylketone intermediates (18). Treatment of diazomethylketone intermediates (18) with lithium chloride in aqueous acetic acid provides the protected tetrahydroforo[3,2-6]pyrrol-3-one (19). Introduction of simple R1 substituents may be achieved by condensation of activated (17) with alternatives to diazomethane such as diazoethane (R1 = CHs), or 1-phenyloxydiazoethane (R1 =
The protected building blocks (synthesis exemplified by the tetrahydrofuro[3,2-&]-pyrrol-3-one (19)) detailed in Scheme 1 may be utilised in a solid phase synthesis of inhibitor molecules (steps (b) to (e)). Step (b), the solid phase linkage of an aldehyde or ketone, has previously been described by a variety of methods (e.g. see (a) James, I. W., 1999, (b) Lee, A., Huang, L., Ellman, J. A., J. Am. Chem. Soc, 121(43), 9907-9914, 1999, (c) Murphy, A. M., et al, J. Am. Chem. Soc, 114, 3156-3157, 1992). A suitable method amenable to the reversible linkage of an alkyl ketone functionality such as (19) is through a combination of the previously described chemistries. The semicarbazide, 4-[[(hydrazinocarbonyl)amino]methyl]cyclohexane carboxylic acid. trifluoroacetate (20) (Murphy, A. M., et al, J. Am. Chem. Soc, 114, 3156-3157,
1992), may be utilised as illustrated in Scheme 2, exemplified by linkage of the tetrahydrofuro[3,2-6]pyrrol-3-one(19).

Scheme 2. (a) (19) in 90% EtOH / H2O / 1.5eq NaOAc / 4-[[(hydrazinocarbonyl)amino]methyl]-cyclohexane carboxylic acid-trifluoroacetate (20), 2hr reflux, (b) 3eq construct (21) / 3eq HBTU / 3eq HOBt / 6eqNMM, NH2-SOUDD PHASE, DMF, RT, o/n. (c) 20% piperidine / DMF, 30mins. (d) Range of chemistries to introduce U-V-W-X-Y (e) TFA / H20 (95:5, v/v), RT, 2hr
Construct (21) is prepared through reaction of the linker molecule (20) and the tetrahydrofuro[3,2-£]pyrrol-3-one (19) by reflux in aqueous ethanol / sodium acetate. Standard solid phase techniques (e.g. see Atherton, E. and Sheppard, R. C., 1989) are used to anchor the construct to an amino-functionalised solid phase through the free carboxylic acid functionality of (21), providing the loaded construct (22).
Loaded construct (22) may be reacted with a wide range of carboxylic acids available commercially or in the literature, to introduce the left-hand portion 'U-V-W-X-Y' in general formula (I). In the simplest example, the entire left hand portion of an inhibitor of general formula (I) comprises a capped aminoacid (Scheme 3), providing for example analogues of general formula (I) where R12 = 'H', (X)0 = '-', (W)n = 'NH', R16 = 'H', n = 1, (V)m = 'CO', m = 1 and U = aryl
40eq NMM, DMF, o/n (c) 5eq carboxylic acid / 5eq HBTU / 5eq HOBt / lOeq NMM, DMF, RT, o/n (d) TFA / H20 (95:5, v/v), RT, 2hr.
Alternatively, carboxylic acids can be prepared in solution by traditional organic chemistry methods and coupled to construct (22) on the solid phase (Schemes 4-8). For example (Scheme 4), treatment in solution of an amino acid, exemplified by (24) with sodium nitrite / t^SCU, provides the a-hydroxyacid, exemplified by (25) (Degerbeck, F. et al, J. Chem. Soc, Perkin Trans. J, 11-14,1993). Treatment of cc-hydroxyacid, (25) with sodium hydride in a dimethylformamide / dichloromethane mixture followed by addition of benzyl bromide, provides IRS-benzyloxy-S-cyclohexylpropionic acid (26). Coupling of (26) to the solid phase construct (22) followed by cleavage, provides (27), an example of general formula (I) where R12 -'H', (X)0 = '-', (W)n = 'O', n = 1, (V)m = 'CH2', i.e. R17, R18 = 'H', m * 1 and U =
phenyl. To those skilled in the practices of organic synthesis, a wide variety of aminoacids such as (24) may be converted to the corresponding a-hydroxyacid such as (25) following the general conditions detailed. Additionally, benzylbromide may be replaced by any reasonable Ar-CR17R18-halide, providing many variations of carboxylic acid (26) following the general conditions detailed. In certain instances, it may be advantageous to temporarily protect the carboxylic acid as the methyl ester (for example compound (32), Scheme 6) prior to reaction with the alkylhalide. The ester intermediate is then simply hydrolysed to acid (26). Analogues of (27), exploring a wide range of (V)m and U in general formula (I) may be prepared through the general conditions detailed in Scheme 4. Since the final synthetic step involves a trifluoroacetic acid (TFA) mediated cleavage of the solid phase bound compound,
analogues where the substituted ether is labile to TFA may be prepared in solution by an alternative route (see Scheme 11).

Scheme 4. (a)NaNO2 / H2SO4,0°C-»RT, 2hr (b) 2.3eqNaH, 1:1 DMF /DCM, 1.4eqbenzylbromide, o/n (c) 20% piperidine / DMF, SOmins. (d) lOeq (26) / lOeq HBTU / lOeq HOBt / 20eq NMM, DMF, RT, o/n (e) TFA / H2O (95:5, v/v), RT, 2hr.
Alternatively, coupling of construct (22) (following removal of Fmoc) with the ct-hydroxyacid (25), provides a versatile solid phase bound intermediate' Y' substituent in general formula (I) that may be reacted with many reagents. For example, the o> hydroxyl can be reacted under Mitsunobu conditions (Hughes, D. L. Org. React.(N.Y), 42, 335-656, 1992) to give ethers (i.e. X = '-', W = 'O', in general formula (I)) (see Grabowska, U. et al, J. Comb. Chem., 2(5), 475-490, 2000, for an example of Mitsunobu reaction on the solid phase). Alternatively, the a-hydroxyl can be reacted with a carbamoyl chloride to give a carbamate (i.e. X = '-', W = *O', V = 'NHC(O)', in general formula (I)).
Alternatively, (Scheme 5), treatment in solution of an ammo acid, exemplified by (24) with sodium nitrite / H2S04 / potassium bromide provides the a-bromoacid, exemplified by (28) (Souers, A. J. et al, Synthesis, 4, 583-585,1999) with retention of configuration. Treatment of a-bromoacid (28) with an alkylthiol exemplified by 4-^rt-butylphenylmethanethiol (29) in dimethylformamide / triemylamine, provides
25r-(4-tert-butylbenzylsulfanyl)-4-methylpropionic acid (30), with inversion of configuration. Coupling of (30) to the solid phase construct (22) followed by cleavage, provides (31), an example of general formula (I) where R12 = 'H', (X)0 = '-', (W)n = 'S', n = 1, (V)m = 'CH2', i.e. R17, R18 = 'H', m = 1 and U = 4-tert-butylphenyl. To those skilled in the practices of organic synthesis, a wide variety of aminoacids such as (24) may be converted to the corresponding a-bromoacid such as (28) following the general conditions detailed. Additionally, starting with the S-isomer of (24) gives the S-bromoacid analogue of (28) and jR-thioether analogue of (30). Additionally, (4-ter/-butylphenyl)methanethiol (29) may be replaced by any reasonable Ar-CR17R18-SH, providing many variations of carboxylic acid (30) following the general conditions detailed. Thus analogues of (31) exploring a wide range of (V)m and U in general formula (I) may be prepared through the general conditions detailed in Scheme 5.

Scheme 5. (a)NaNO2 / H2SO4l KBr 0°C-»RT, 2hr (b) Alkylthiol (29) / DMF / NEt3) o/n (c) 20% pipendine / DMF, SOmins. (d) lOeq (30) / lOeq HBTU / lOeq HOBt / 20eq NMM, DMF, RT, o/n (e) TFA / H2O (95:5, v/v), RT, 2hr.
Alternatively, coupling of construct (22) (following removal of Fmoc) with an a-bromoacid e.g. (28), provides a versatile intermediate 'Y' substituent hi general formula (I) that may be reacted with many reagents. For example, the a-bromide can be displaced with nucleophiles e.g. alcohols, thiols, carbanions etc, to give ethers (i.e. X = '-', W = 'O', in general formula (I)), thioethers (i.e. X = '-', W = 'S', in general
formula (I)). The thioethers may optionally be oxidised to the sulphone (see Scheme 9, i.e. X = '-', W = 'SO2', in general formula (I)) (see Grabowska, U. et al, J. Comb. Chem., 2(5), 475-490, 2000, for an example of bromide displacement and tbioether oxidation on the solid phase).
Alternatively, (Scheme 6), treatment of an a-hydroxyacid, exemplified by (25) with trimethylsilylchloride and methanol provides the methyl ester (32). Activation of the free hydroxyl to the chloroformate with phosgene in dichloromethane followed by addition of morpholine, then hydrolysis, provides morpholine-4-carboxylic acid-15-carboxy-2-cyclohexyl ethyl ester (33). Coupling of (33) to the solid phase construct (22) followed by cleavage, provides (34), an example of general formula (I) where R12 = 'H', (X)0 = '-', (W)n = 'O', n = 1, (V)m = 'CO' and U = morpholino. To those skilled in the practices of organic synthesis, a wide variety of a-hydroxyacid esters such as (32) could be converted to the activated chloroformate following the general conditions detailed. Additionally, morpholine may be replaced by any reasonable amine, providing many variations of carboxylic acid (33) following the general conditions detailed. Thus analogues of (34) exploring a wide range of (V)m and U in general formula (I) may be prepared through the general conditions detailed in Scheme 6.
Altematively, (Scheme 7), a wide range of alkylsuccinate esters exemplified by 2R-cydohexylmethylsuccinic acid 1-methyl ester (35) are commercially available or readily prepared by known methods (see (a) Azam et al, J. Chem. Soc. Perkin Trans. 1, 621-, 1996; (b) Evans et al, J. Chem. Soc. Perkin Trans. 1, 103, 2127, 1981; (c) Oikawa et al, Tet. Lett, 37, 6169,1996). Carboxyl activation of alkylsuccinate ester
(35) followed by addition of morpholine in dimethylformamide and subsequent ester
hydroylsis, provides 2/?-cyclohexylmethyl-4-morpholin-4-yl-4-oxo-butyric acid (36).
Coupling of (36) to the solid phase construct (22) followed by cleavage, provides
(37), an example of general formula (I) where R12 = 'H', (X)0 = 'CH2' i.e. R14, R15 =
'H', o=l, (W)n = 'CO', n = 1, (V)m = '-' and U = morpholino. To those skilled in
the practices of organic synthesis, a wide variety of alkylsuccinate esters such as (35)
may be prepared and converted to the corresponding substituted alkylsuccinate acid
such as (36) following the general conditions detailed. Additionally, morpholine may
be replaced by any reasonable amine, providing many variations of carboxylic acid
(36) following the general conditions detailed. Thus analogues of (37) exploring a
wide range of (X)0, (V)m and U in general formula (I) may be prepared through the
general co
Scheme 7. (a) LEDC / 1-hydroxybenzotriazole /DMF, 0°C, SOmins. ii. Morpholine, RT, o/n (b) LiOH in H20 / dioxan, 0°C (c) 20% piperidine / DMF, SOmins. (d) lOeq (36) / lOeq HBTU / lOeq HOBt / 20eq NMM, DMF, RT, o/n (e) TFA / H20 (95:5, v/v), RT, 2hr.
Alternatively, (Scheme 8), a wide range of biarylalkylacetic acids, exemplified by 2&S-biphenyl-3-yl-4-methylpentanoic acid (39) are readily available by known methods (see (a) DesJarlais, R. L. et al, J. Am. Chem. Soc, 120. 9114-9115,1998; (b) Oballa, R. M. et al, WO 0149288). Coupling of biarylalkylacetic acid (39) to the solid phase construct (22) followed by cleavage, provides (40), an example of general formula (I) where R12 = 'H', (X)0 = '-', (W)n = '-', (V)m = '-' and U = m-biphenyl. To those skilled in the practices of organic synthesis, a wide variety of biarylalkylacetic acids such as (39) may be prepared by alkylation of the a-anion of the free acid analogue of (38), which in turn is prepared by Suzuki coupling of phenylboronic acid and 3-bromophenylacetic acid methyl ester. Phenylboronic acid may be replaced by a wide range of arylboronic acids in the Suzuki coupling, providing many variations of carboxylic acid (39) following the general conditions detailed. Thus analogues of (40) exploring a wide range of group 'U' in general formula (I) may be prepared through the general conditions detailed in Scheme 8.
Scheme 8. (a) LiOH in H2O / dioxan, 0°C (b) LLDA, THF, 2-methylpropenylbromide. ii. Pd/C, EtOH, H2 (c) 20% piperidine / DMF, 30mins. (d) lOeq (39) / lOeq HBTU / lOeq HOBt / 20eq NMM, DMF, RT, o/n (e) TFA / H2O (95:5, v/v), RT, 2hr.
Many other possibilities for solid phase organic chemistry (e.g. see Brown, R. D. J. Chem. Soc., Perkin Trans. 1, 19, 3293-3320, 1998, for a review of recent SPOC publications) can be used to derivatise construct (22) towards compounds of general formula (I) (and similarly, through the appropriate loaded derivative, compounds of general formula (II)). For example, the left-hand portion 'U-V-W-X-Y1 in general formulae (I) can be partially constructed in solution, coupled to construct (22) and further modified on the solid phase. For example (Scheme 9), a simple extension of Scheme 5 is through the oxidation of the intermediate solid phase bound species, with m-chloroperbenzoic acid in dichloromethane prior to cleavage, to give the sulphone analogue (42). Commencing from a variation of carboxylic acid (30), 3-cyclohexyl-25-(furan-2-ylmethylsulfanyl)propionic acid (41), sulphone (42) is prepared, an example of general formula (I) where R12 = 'H', (X)0 = '-', (W)n = 'S02', n = 1, (V)m = 'CH2', i.e. R17, R18 = 'H', m = 1 and U = 2-furanyL As described in Scheme 5, many variations of carboxylic acid (30) may be prepared following the general conditions detailed e.g. (41). Thus analogues of (42) exploring a wide range of (V)m and U in general formula (I) may be prepared through the general conditions detailed in Schemes 5 and 9.
Compounds of general formulae (I) and (II) are finally released from the solid phase by treatment with trifhioroacetic acid / water, followed by evaporation, lyophylis and standard analytical characterisation.
A second strategy for the synthesis of compounds of general formulae (I) and (II) comprises:-
(a) Preparation of an appropriately functionalised and protected
tetrahydrofuro[3,2-6]pyrrol-3-one, tetrahydrothieno[3,2-£]pyrrol-3-one,
hexahydropyrrolo[3,2-6] pyrrol-3-one, hexahydrocyclopenta[fc]pyrrol-6-one,
tetrahydrofuro[3,2-c]pyrazol-6-one, tetrahydrothienop^-cjpyrazol-e-one,
hexahydropyrrolo[3,2-c]pyrazol-6-one, hexahydrocyclopentapyrazol-6-one,
hexahydrofuro[3,2-fc]pyridin-3-one, hexahydrothieno[3,2-&]pyridin-3-one,
octahydropyrrolo[3,2-6] pyridin-3-one, octahydrocyclopenta[6]pyridin-7-one,
hexahydrofuro[3,2-c]pyridazin-7-one, hexahydrothieno[3,2-c]pyridazm-7-
one, octahydropyrrolo[3,2-c]pyridazin-7-one or
octahydrocyclopenta[c]pyrida2in-7-one building block in solution.
Preferred protecting groups for solution phase chemistry are the Na-terf-
butoxycarbonyl group and the Na-benzyloxycarbonyl group.
(b) Standard organic chemistry methods for the conversion of building block (a)
towards compounds of general formulae (I) and (II).
In the simplest example, the entire left hand portion of an inhibitor of general formulae (I) and (II) can be prepared in solution by traditional organic chemistry methods and coupled to building block (a) (see Scheme 10 exemplified by preparation and use of the 3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carboxyUc acid tert-butyl ester (45)).
cheme 10. (a) 'BuOCOCl, NMM, DCM, -15°C, lOmins, under argon, (b) Diazomethane in diethyl ether, -15°C to RT over 24hr. (c) Acetic acid (d) Lid (lOeq) in 80%aq acetic acid, 5°C to RT over Ihr. (e) 4M HCI in dioxan, 0°C, 2hrs. (f) Pre-prepared U-V-W-X-Y-COOH / activation e.g. HATU / HOAt / NMM, DMF, RT, o/n.
The general strategy detailed in Scheme 10 is particularly useful when the compound of general formula (I) contains a substituent that is labile to trifluoroacetic acid, this being the final reagent used in each of the solid phase Schemes 4-9. For example (Scheme 11), treatment in solution of a-hydroxyacid (47) with sodium hydride in a dimethylformamide / dichloromethane mixture followed by addition of 4-tert-butylbenzyl bromide, provides 2J?5'-(4-rer?-butylbenzyloxy)-4-methylpentanoic acid (48). Coupling of (48) to hydrochloride salt (46), provides (49), an example of general formula (I) where R12 = 'H', (X)0 = '-', (W)n = 'O', n = 1, (V)m = 'CH2', i.e. R17, R18 = 'H', m = 1 and U = 4-tert-butylphenyl. To those skilled in the practices of organic synthesis, 4-tert-butylbenzyl bromide may be replaced by any reasonable Ar-CR17R18-halide, providing many variations of carboxylic acid (48) under the conditions shown. Thus analogues of (49) exploring a wide range of (V)m and U in general formula (I) may be prepared through the conditions detailed in Scheme 11.
Scheme 11. (a)2.2eq NaH, 1:1 DMF / DCM, 1.25eq 4-tert-benzylbromide, 2hr (b)leq (48), leq teuOCOCl, 2eqNMM, DCM, -15°C, Ihr, under nitrogen, then leq, (46), RT, o/n.
A third strategy for the synthesis of compounds of general formulae (I) and (II) where the addition of U-V-W-X-Y to the protected building block involves multistep organic reactions comprises:-
(a) Preparation of an appropriately functionalised and protected
tetrahydrofuro[3,2-&]pyrrol-3-one, tetrahydrothieno[3,2-fe]pyrrol-3-one,
hexahydropyrrolo[3,2-6]pyrrol-3-one, hexahydrocyclopenta[&]pyrrol-6-one,
tetrahydrofuro[3,2-c]pyrazol-6-one, tetrahydrothieno[3,2-c]pyrazol-6-one,
hexahyd^opyrrolo[332Tc]pyrazol-6-one, hexahydrocyclopentapyrazol-6-one,
hexahydrofuro[3,2-i]pyridin-3-one, hexahydrothieno[3,2-&]pyridin-3-one,
octahydropyrrolo[3,2-i] pyridin-3-one, octahydrocyclopenta[i]pyridin-7-one,
hexahydrofuro[3j2-c]pyridazin-7-one., hexahydrothieno[3,2-c]pyridazin-7-
one, octahydropyrroloCS^-clpyridazin^-one or octahydrocyclopenta[c]
pyridazin-7-one building block in solution.
Preferred protecting groups for solution phase chemistry are the Na,-tert-butoxycarbonyl group and the Na-benzyloxycarbonyl group.
(b) Protection of the ketone functionality of the tetrahydrofuro[3,2-6]pyrrol-3-
one, tetrahydrothieno[3,2-6]pyrrol-3-one, hexahydropyrrolo[332-6]pyrrol-3-
one, hexahydrocyclopenta[6]pyrrol-6-one3 tetrahydrofuro[3,2-c]pyrazol-6-
one, tetrahydrothieno[3,2-c]pyrazol-6-one, hexahydropyrrolo[3,2-c]pyrazol-6-one, hexahydrocyclopentapyrazol-6-one, hexahydrofuro[3,2-&]pyridin-3-one, hexahydrothieno[3,2-&]pyridin-3-one, octahydropyrrolo[3,2-&]pyridin-3-one, octahydrocyclopenta[6]pyridin-7-one, hexahydrofuro[3,2-c]pyridazin-7-one, hexaliydrothieno[3,2-c]pyridazin-7-one, octahydropyrrolo[3,2-c]pyridazin-7-one or octahydrocyclopenta[c]pyridazin-7-one building block e.g. as a dimethylacetal. Alternatively, the ketone may be reduced to the achiral secondary alcohols and re-oxidised as the final synthetic step.
(c) Standard organic chemistry methods for the conversion of building block (b)
towards compounds of general formulae (I) and (II).
(d) Intermediates may be prepared in solution, followed by coupling to building
block (b) and further derivitisation towards compounds of general formulae
(I) and (II) (see Scheme 12 exemplified by preparation and use of the 3-
hydroxyhexahydrofuro[3,2-6]pyrrole-4-carboxylic acid tert-butyl ester (50)).
Alternatively, depending upon the types of chemistry used to construct the left hand side U-V-W-X-Y of compounds of general formula (I) and (II), the ketone may require protection e.g. as the dimethyl acetal. Such a method is detailed and exemplified in Scheme 13 by the preparation and use of 3,3-dimethoxyhexahydrofur
Scheme 13. (a) Triethylorthofonnate / pTSA / MeOH. (b) H2, Pd-C. (c) Stepwise reaction with intennediates of Y, then X, then W etc., to stepwise construct compounds (56). (d) Trifluoroacetic acid/CH2Cl2/H20.
The invention extends to novel intermediates as described above, and to processes for preparing compounds of general formulae (I) or (II) from each of their immediate precursors. In turn, processes for preparing intermediates from their immediate precursors also form part of the invention.
Compounds of general formulae (I) and (II) are useful both as laboratory tools and as
^
therapeutic agents. In the laboratory certain compounds of the invention are useful in establishing whether a known or newly discovered cysteine protease contributes a critical or at least significant biochemical function during the establishment or progression of a disease state, a process commonly referred to as 'target validation' .
According to a second aspect of the invention, there is provided a method of validating a known or putative cysteine protease inhibitor as a therapeutic target, the method comprising:
(a) assessing the in vitro binding of a compound as described above to an isolated
known or putative cysteine protease, providing a measure of potency; and optionally,
one or more of the steps of:
(b) assessing the binding of the compound to closely related homologous proteases
of the target and general house-keeping proteases (e.g. trypsin) to provides a measure
of selectivity;
(c) monitoring a cell-based functional marker of a particular cysteine protease
activity, in the presence of the compound; and
(d) monitoring an animal model-based functional marker of a particular cysteine
protease activity in the presence of the compound.
The invention therefore provides a method of validating a known or putative cysteine protease inhibitor as a therapeutic target. Differing approaches and levels of complexity are appropriate to the effective inhibition and 'validation' of a particular target. In the first instance, the method comprises assessing the in viti'o binding of a compound of general formulae (I) or (II) to an isolated known or putative cysteine protease, providing a measure of 'potency'. An additional assessment of the binding of a compound of general formulae (I) or (IT) to closely related homologous proteases of the target and general house-keeping proteases (e.g. trypsin) provides a measure of 'selectivity'. A second level of complexity may be assessed by monitoring a cell-based functional marker of a particular cysteine protease activity, in the presence of a compound of general formulae (I) or (II). For example, a 'human osteoclast resorption assay' has been utilised as a cell-based secondary in vifro testing system for monitoring the activity of cathepsin K and the biochemical effect of protease inhibitors (e.g. see WO-A-9850533). An 'MHC-II processing - T-cell

-119-
activation assay' has been utilised as a cell-based secondary in vitro testing system for monitoring the activity of cathepsin S and the biochemical effect of protease inhibitors (Shi, G-P., et at, Immunity, 10, 197-206,1999). When investigating viral or bacterial infections such a marker could simply be a functional assessment of viral (e.g. count of mRNA copies) or bacterial loading and assessing the biochemical effect of protease inhibitors. A third level of complexity may be assessed by monitoring an animal model-based functional marker of a particular cysteine protease activity, in the presence of a compound of general formulae (I) or (II). For example, murine models of Leishmania infection, P. vinckei infection, malaria (inhibition of falcipain) and T. cnizi infection (cruzipain), indicate that inhibition of cysteine proteases that play a key role in pathogen propagation is effective in arresting disease symptoms, 'validating' said targets.
The invention therefore extends to the use of a compound of general formulae (I) or (H) in the validation of a known or putative cysteine protease inhibitor as a therapeutic target.
Compounds of general formulae (I) and (II) are useful for the in vivo treatment or prevention of diseases in which participation of a cysteine protease is implicated.
According to a third aspect of the invention, there is provided a compound of general formulae (I) or (II) for use in medicine, especially for preventing or treating diseases in which the disease pathology may be modified by inhibiting a cysteine protease.
According to a fourth aspect of the invention, there is provided the use of a compound of general formulae (I) or (II) in the preparation of a medicament for preventing or treating diseases in which the disease pathology may be modified by inhibiting a cysteine protease.
Certain cysteine proteases function in the normal physiological process of protein degradation in animals, including humans, e.g. in the degradation of connective tissue. However, elevated levels of these eirzymes in the body can result in

jathological conditions leading to disease. Thus, cysteine proteases have been mplicated in various disease states, including but not limited to, infections by °neumocystis carinii, Trypsanoma cruzi, Trypsanoma brucei brucei and Criihidia The invention is useful in the prevention and/or treatment of each of the disease states mentioned or implied above. The present invention also is useful in a methods of treatment or prevention of diseases caused by pathological levels of cysteine proteases, particularly cysteine proteases of the papain superfamily, which methods comprise administering to an animal, particularly a mammal, most particularly a human, in need thereof a compound of the present invention. The present invention particularly provides methods for treating diseases in which cysteine proteases are implicated, including infections by Pneumocystis carinii, Trypsanoma cntzi, Trypsanoma brucei, Leishmania mexicana, Clostridium histofyticum, Staphylococcus aureus, foot-and-mouth disease virus and Criihidia fusiculata; as well as in osteoporosis, autoimmunity, schistosomiasis, malaria, tumour metastasis, metachromatic leukodystrophy, muscular dystrophy and amytrophy.
Inhibitors of cruzipain, particularly cruzipain-specific compounds, are useful for the treatment of Chagas' disease.
In accordance with this invention, an effective amount of a compound of general formulae (I) or (H) may be administered to inhibit the protease implicated with a particular condition or disease. Of course, this dosage amount will further be modified according to the type of administration of the compound. For example, to achieve an "effective amount" for acute therapy, parenteral administration of a compound of general formulae (I) or (II) is preferred. An intravenous infusion of the

compound in 5% dextrose in water or normal saline, or a similar formulation with suitable excipients, is most effective, although an intramuscular bolus injection is also useful. Typically, the parenteral dose will be about 0.01 to about 100 mg/kg; preferably between 0.1 and 20 mg/kg, in a manner to maintain the concentration of drug in the plasma at a concentration effective to inhibit a cysteine protease. The compounds may be administered one to four times daily at a level to achieve a total daily dose of about 0.4 to about 400 mg/kg/day. The precise amount of an inventive -compound which is therapeutically effective, and the route by which such compound is best administered, is readily determined by one of ordinary skill in the art by comparing the blood level of the agent to the concentration required to have a therapeutic effect. Prodrugs of compounds of the present invention may be prepared by any suitable method. For those compounds in which the prodrug moiety is. a .ketone functionality, specifically ketals and/or hemiacetals, the conversion may be effected in accordance with conventional methods.
The compounds of this invention may also be administered orally to the patient, in a manner such that the concentration of drug is sufficient to inhibit bone resorption or to achieve any other therapeutic indication as disclosed herein. Typically, a pharmaceutical composition containing the compound is administered at an oral dose of between about 0.1 to about 50 mg/kg in a manner consistent with the condition of the patient. Preferably the oral dose would be about 0.5 to about 20 mg/kg.
No unacceptable toxicological effects are expected when compounds of the present invention are administered in accordance with the present invention. The compounds of this invention, which may have good bioavailability, may be tested in one of several biological assays to determine the concentration of a compound which is required to have a given pharmacological effect.
According to a fifth aspect of the invention, there is provided a pharmaceutical or veterinary composition comprising one or more compounds of general formulae (I) or (II) and a pharmaceutically or veterinarily acceptable carrier. Other active
materials may also be present, as may be considered appropriate or advisable for the disease or condition being treated or prevented.
The carrier, or, if more than one be present, each of the carriers, must be acceptable in the sense of being compatible with the other ingredients of the formulation and not deleterious to the recipient.
The formulations include those suitable for rectal, nasal, topical (including buccal and sublingual), vaginal or parenteral (including subcutaneous, intramuscular, intravenous and intradermal) administration, but preferably the formulation is an orally administered formulation. The formulations may conveniently be presented in unit dosage form, e.g. tablets and sustained release capsules, and may be prepared by any methods well known in the art of pharmacy.
Such methods include the step of bringing into association the above defined active agent with the carrier. In general, the formulations are prepared by uniformly and intimately bringing into association the active agent with liquid carriers or finely divided solid carriers or both, and then if necessary shaping the product. The invention extends to methods for preparing a pharmaceutical composition comprising bringing a compound of general formulae (I) or (II) in conjunction or association with a pharmaceutically or veterinarily acceptable carrier or vehicle.
Formulations for oral administration in the present invention may be presented as: discrete units such as capsules, cachets or tablets each containing a predetermined amount of the active agent; as a powder or granules; as a solution or a suspension of the active agent in an aqueous liquid or a non-aqueous liquid; or as an oil-in-water liquid emulsion or a water in oil liquid emulsion; or as a bolus etc.
For compositions for oral administration (e.g. tablets and capsules), the term "acceptable carrier" includes vehicles such as common excipients e.g. binding agents, for example syrup, acacia, gelatin, sorbitol, tragacanth, polyvinylpyrrolidone (Povidone), methylcellulose, ethylcellulose, sodium carboxymethylcellulose, hydroxypropylmethylcellulose, sucrose and starch; fillers and carriers, for example
com starch, gelatin, lactose, sucrose, microcrystalline cellulose, kaolin, mannitol, dicalcium phosphate, sodium chloride and alginic acid; and lubricants such as magnesium stearate, sodium stearate and other metallic stearates, glycerol stearate stearic acid, silicone fluid, talc waxes, oils and colloidal silica. Flavouring agents such as peppermint, oil of wintergreen, cherry flavouring and the like can also be used. It may be desirable to add a colouring agent to make the dosage form readily identifiable. Tablets may also be coated by methods well known in the art. A tablet may be made by compression or moulding, optionally with one or more accessory ingredients. Compressed tablets may be prepared by compressing in a suitable machine the active agent in a free flowing form such as a powder or granules, optionally mixed with a binder, lubricant, inert diluent, preservative, surface-active or dispersing agent. Moulded tablets may be made by moulding in a suitable machine a mixture of the powdered compound moistened with an inert liquid diluent. The tablets may be optionally be coated or scored and may be formulated so as to provide slow or controlled release of the active agent.
Other formulations suitable for oral administration include lozenges comprising the active agent hi a flavoured base, usually sucrose and acacia or tragacanth; pastilles comprising the active agent in an inert base such as gelatin and glycerin, or sucrose and acacia; and mouthwashes comprising the active agent in a suitable liquid carrier.
Parenteral formulations will generally be sterile.
According to a sixth aspect of the invention, there is provided a process for the preparation of a pharmaceutical or veterinary composition as described above, the process comprising bringing the active compound(s) into association with the carrier, for example by admixture.
Preferred features for each aspect of the invention are as for each other aspect mutatis mutandis.
The invention will now be illustrated with the following examples:
Solution Phase Chemistry - General Methods
All solvents were purchased from ROMIL Ltd (Waterbeach, Cambridge, UK) at SpS or Hi-Dry grade unless otherwise stated. General peptide synthesis reagents were obtained from Chem-Impex Intl. Inc. (Wood Dale IL 60191. USA). Thin layer chromatography (TLC) was performed on pre-coated plates (Merck aluminium sheets silica 60 F254, part no. 5554). Visualisation of compounds was achieved under ultraviolet light (254nm) or by using an appropriate staining reagent. Flash column purification was performed on silica gel 60 (Merck 9385). All analytical HPLC were obtained on Phenomenex Jupiter C4, 5ji, 300A, 250 x 4.6mm, using mixtures of solvent A = 0.1 %aq trifluoroacetic acid (TFA) and solvent B = 90% acetonitrile /10% solvent A on automated Agilent systems with 215 and / or 254nm UV detection. Unless otherwise stated a gradient of 10 - 90% B in A over 25 minutes at 1.5mL / min was performed for full analytical HPLC analysis. HPLC-MS analysis was performed on an Agilent 1100 series LC/MSD, using automated Agilent HPLC systems, with a gradient of 10 - 90% B in A over 10 minutes on Phenomenex Columbus Cg, 5|i, 300A, 50 x 2.0mm at 0.4mL / min. Nuclear magnetic resonance (NMR) were obtained on a Bruker DPX400 (400MHz 1H frequency; QXI probe) in the solvents and temperature indicated. Chemical shifts are expressed in parts per million (5) and are referenced to residual signals of the solvent. Coupling constants (J) are expressed in Hz.
Solid Phase Chemistry - General Methods
Example inhibitors (1-156) were prepared through a combination of solution and solid phase Fmoc-based chemistries (see 'Solid Phase Peptide Synthesis', Atherton, E. and Sheppard, R. C., IRL Press Ltd, Oxford, UK, 1989, for a general description). An appropriately protected and functionalised building block was prepared in solution (e.g. general compound (19), Scheme 1), then reversibly attached to the solid phase through an appropriate linker. Rounds of coupling / deprotection / chemical modification e.g. oxidation were then performed until the full length
desired molecule was complete (Scheme 2). Example inhibitors (1-156) were then released (cleaved) from the solid phase, analysed, purified and assayed for inhibition verses a range of proteases.
Generally, multipins (polyamide 1.2 -> lOjimole loadings, see www.mimotopes.com) were used for the solid phase synthesis, although any suitable solid phase surface could be chosen. In general, the 1.2|imole gears were used to provide small scale crude examples for preliminary screening, whilst the 10 ^mole crowns were used for scale-up synthesis and purification of preferred examples. Standard coupling and Fmoc deprotection methods were employed (see Grabowska, U. et al, J. Comb. Chem. 2(5), 475-490, 2000. for a thorough description of solid phase multipin methodologies).
Preparation of Initial Assembly
Building Block-linker constructs (e.g.(21), typically lOOmg to 2g) were carboxyl activated with 2-(lH-benzotriazole-l-yl)-l,l,3,3-tetramethyluronium hexafluoro phosphate (HBTU, 1 mole equivalent), 1-hydroxybenzotriazole.hydrate (HOST, 1 mole equivalent) and N-methyhnorpholine (NMM, 2 mole equivalents) in dimethylformamide (DMF, typically 1 to lOmL) for 5 minutes. Ammo functionalised DA/MDA crowns or HEMA gears (lOjjmole per crown / 1.2umole per gear, 0.33 mole equivalent of total surface amino functionalisation compared to activated construct) were added, followed by additional DMF to cover the solid phase surface. The loading reaction was left overnight. Following overnight loading, crowns / gears were taken through standard cycles washing, Fmoc deprotection and loading quantification (see Grabowska, U. et al) to provide loaded Building Block-linker constructs (e.g.(22)). Analysis indicated virtually quantitative loading in all examples.
Coupling Cycles
The coupling of standard Fmoc-aminoacids (10 or 20 mole equivalent) were performed via carboxyl activated with 2-(lH4>enzotriazole-l-yl)-l,1,3,3-tetramethyluronium hexafluoro phosphate (HBTU, 10 or 20mole equivalent), 1-hydroxybenzotriazole.hydrate (HOBT, 10 or 20mole equivalent) and N-methylmorpholine (NMM, 20 or 40mole equivalents) in dimethylformamide, with pre-activation for 5 minutes. Activated species were dispensed to the appropriate wells of a polypropylene 96-well plate (Beckman, ImL wells, 500}iL solution per well for crowns or 250fj,L solution per well for gears) in a pattern required for synthesis. Loaded free arnino Building Block-linker constructs (e.g.(22)) were added and the coupling reaction left overnight. Following overnight coupling, crowns / gears were taken through standard cycles washing and Fmoc deprotection (see Grabowska, U. et al). Identical activation and coupling conditions were used for the coupling of a range of carboxylic acids (R-COOH). Alternatively, chloroformates e.g. morpholine-4-carbonylchloride (lOmole equivalent), were coupled in DMF with the addition of NMM (lOmole equivalents).
Acidolytic Cleavage Cycle
A mixture of 95% TFA / 5% water was pre-dispensed into two polystyrene 96-well plates (Beckman, ImL wells, 600uL solution per well for crowns or 300uL solution per well for gears) in a pattern corresponding to that of the synthesis. The completed multipin assembly was added to the first plate (mother plate), the block covered in tin foil and cleaved for 2 hours. The cleaved multipin assembly was then removed from the first plate and added to the second plate (washing plate) for 15 minutes. The spent multipin assembly was then discarded and the mother / washing plates evaporated on an HT-4 GeneVac plate evaporator.
Analysis and Purification of Cleaved Examples
(a) Ex 1.2umole Gears. lOOuL dirnethylsulphoxide (DMSO) was added to each post cleaved and dried washing plate well, thoroughly mixed, transferred to the corresponding post cleaved and dried mother plate well and again thoroughly
mixed. lOuL of this DMSO solution was diluted to lOOuL with a 90% acetonitrile /10% 0.1 %aq TFA mixture. 20uL aliquots were analysed by HPLC-MS and full analytical HPLC. In each case the crude example molecules gave the expected [M + H]+ ion and an HPLC peak at > 80% (by 215nm UV analysis). This provided an approximately lOmM DMSO stock solution of good quality crude examples for preliminary protease inhibitory screening.
(b) Ex lOfimole Crowns. SOOuL of a 90% acetonitrile / 10% 0.1 %aq TFA mixture
was added to each washing plate well, thoroughly mixed, transferred to the
corresponding mother plate well and again thoroughly mixed. 5uL of this
solution was diluted to lOOuL with a 90% acetonitrile / 10% 0.1%aq TFA
mixture. 20uL aliquots were analysed by HPLC-MS and full analytical HPLC. In
each case the crude example molecules gave the expected [M + H]+ ion and an
HPLC peak at > 80% (by 215nm UV analysis). The polystyrene blocks
containing crude examples were then lyophilised.
(c) Individual examples (ex (b)) were re-dissolved in a 1 :1 mixture of 0.1% aq TFA
/ acetonitrile (ImL) and purified by semi-preparative HPLC (Phenomenex Jupiter
C4, 5\i, 300A, 250 x 10mm, a 25-90% B in A gradient over 25mins, 4.0mL/min,
215nm UV detection). Fractions were lyophilised into pre-tarred glass sample
vials to provide purified examples (typically 2 to 4mg, 40 to 80% yield).
(d) Purified examples were dissolved hi an appropriate volume of DMSO to provide
a lOmM stock solution, for accurate protease inhibitory screening.
EXAMPLE 1. (An example of general formula (I)). (3aS, 6aK) N-[\S-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]benzamide
Following the general details from Scheme 1, the required bicycle building block (3a5,6aZ?) 3-oxo-hexahydrofuro[3,2-6]pyrrole~4-carboxylic acid 9J?-fluoren-9-yl methyl ester (19) was prepared in 8 steps as follows.
(1) Preparation of (2S,3S) (3-hydroxy)pyrrolidine-l,2-dicarboxylic acid 1-(9H-fluoren-9-ylmethyl) ester.
Trans-3-hydroxy-L-proline (10.Og, 76.3mmole) was added to a vigorously stirred, ice-cooled solution of sodium carbonate (16.90g, 160.2mmole) in water (lOOmL). 1,4-Dioxan (75mL) was added providing an opaque but mobile mixture. 9-Fluorenylmethyl chloroformate (20.3 Ig, SOmmole) in 1,4-dioxan (75mL) was added over Ihr, then the ice-cooling removed and the mixture stirred at RT for an additional 2hr. Additional water (300mL) was added, the reaction mixture washed with chloroform (2 x 250mL) and the combined organic layers discarded. The aqueous phase was acidified with IN HC1 to ~ pH 2, providing a thick opaque mixture. The acidified aqueous mixture was extracted with chloroform (2 x SOOmL) and the now clear aqueous phase discarded. The opaque combined chloroform layers were dried (Na2SC>4), filtered and reduced in vacuo to provide batch 1 (5.70g). The residual precipitate (a mixture of product and drying agent) was triturated with hot methanol (2 x 250mL) and the combined methanol solutions reduced in vacuo to provide batch 2 (10.25g). Batch 1 and 2 were individually analysed by TLC (single UV spot, Rf = 0.15, 20% MeOH in CHC13), and HPLC-MS (single main UV peak with Rt = 7.069mins, 354.2 [M + H]+, 376.2 [M + Na]+) and found to be identical, giving a combined yield of 15.95g (45.2mmole, 59.2%). Analysis by 1H and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond. 5H (DMSO-d6 at 298K); 1.80-2.02 (2Hy, m), 3.49-3.62 (2H§, rn), 4.12-4.38 (Ha, Hp, Fmoc H-9 and CRg, m), 5.55/5.62 (OH), 7.30-7.31 (2H aromatic, Fmoc H-2 and H-7), 7.35-7.37 (2H aromatic, Fmoc H-3 and H-6), 7.43-7.45 (2H aromatic, Fmoc H-l and H-8), 7.63-7.65 (2H aromatic, Fmoc H-4 and H-5), 12.8-13.0 (COOH).
5C (DMSO-de at 298K); 31.70/32.70 (d, C7), 44.68/45.32 (d, C5), 46.94/46.97 (u, Fmoc C-9), 67.04/67.33 (d, Fmoc CH2), 68.24/68.51 (u, Ca), 73.12/74.23 (u, Cp), 120.49/120.52 (u, Fmoc C-4 and C-5), 125.49/125.58 (u, Fmoc C-l and C-8), 127.50 (u, Fmoc C-2 and C-7), 128.04 (u, Fmoc C-3 and C-6), 140.99/141.09 (q, Fmoc C-4' and C-5'), 144.02/144.16 (q, Fmoc C-l' and C-8'), 154.33/154.54 (q, OCON), 172.10/172.39 (COOH).
(2) Preparation of (25,35) (3-hydroxy)pyrrolidine-lJ2-dicarboxylic acid 2-allyl ester l-(9H-fiuoren-9-ylmethyl) ester.
(25,35) (3-hydroxy)pyrrolidine-l,2-dicarboxylic acid l-(9H~fluoren-9-ylmethyl) ester (10.9g, 30.8mmole) was dissolved in toluene (75mL) in a Dean-Stark apparatus. Allyl alcohol (20mL) was added followed by p-toluenesulphonic acid.hydrate (6.05g, 31.4mmole). The mixture was refmxed for Ihr, cooled and CHCls (300mL) added. The organic layer was washed with NaHCOs (300mL), 0.1N HC1 (300mL) and brine (300mL), then dried (Na2SO4). Filtration and reduction in vacuo gave a pale yellow foam (13.5g). The crude foam was purified over silica gel (150g) eluting with a gradient of heptane : ethyl acetate 3:1 -> 1:1. Desired fractions were combined and reduced in vacuo to a colourless gum yield 10.34g (26.3mmole, 85.4%). TLC (single UV spot, Rf = 0.30, heptane : ethyl acetate 1:1), analytical HPLC Rt = 18.849mins, HPLC-MS (single mam UV peak with Rt = 8.354mins, 394.2 [M + H]+, 416.2 [M + Naf). Analysis by !H and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond. 5H (CDC13 at 298K); 2.00-2.21 (2HY, m), 2.70/2.85 (OH, b), 3.72-3.81 (2HS, m), 4.12-4.67 (Ha, Hp, Fmoc H-9 and CH^,2 x COOCH2CH=CH2, m), 5.20-5.40 (2 x COOCH2CH=CH2, m), 5.82-5.99 (1 x COOCH2CH=CH2, m), 7.28-7.33 (2H aromatic, Fmoc H-2 and H-7), 7.34-7.41 (2H aromatic, Fmoc H-3 and H-6), 7.53-7.66 (2H aromatic, Fmoc H-l and H-8), 7.77-7.81 (2H aromatic, Fmoc H-4 and H-5).
5C (CDC13 at 298K); 32.28/33.04 (d, C?), 44.98/45.32 (d, C8), 47.56/47.63 (u, Fmoc C-9), 66.44 (d, COOCH2CH=CH2), 68.01/68.11 (d, Fmoc CH2), 68.32/68.72 (u, Ca), 74.49/75.67 (u, Cp), 119.20/119.48 (d,-130-
COOCH2CH=CH2), 120.34/120.37 (u, Fmoc C-4 and C-5), 125.36/125.60 (u: Fmoc C-l and C-S), 127.47 (u, Fmoc C-2 and C-7), 128.06/128.12 (u, Fmr -C-3 and C-6), 131.79/131.94 (u, COOCH2CH=CH2), 141.65/141.71 (q, Fmoc C-4' and C-5'), 144.12/144.34 (q, Fmoc C-l' and C-8'), 155.13/155.59 (q, OCON), 170.53/170.55 (COOCH2CH=CH2).
(3) Preparation of (2S,3>R) (3-formyloxy)pyrrolidine-l,2-dicarboxylic acid 2-
allyl ester l-(9H-fluoren-9-ylmethyl) ester.
Triphenylphosphine (2.76g, 10.5mmole, TPP) was dissolved in dry tetrahydrofuran (75mL), under a nitrogen blanket with stirring and ice-cooled. Diisopropylazodicarboxylate (2.12g, 2.07mL, lO.Smmole, DIAD) was added dropwise over ISmins to give a precipitous white mixture. (2S,3S) (3-hydroxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester l-(9H-fluoren-9-ylmethyl) ester (4.15g, lO.Smmole) and formic acid (0.97g, 0.79mL, 2l.0mmole) were mixed in dry tetrahydrofuran (30mL) and added over ISmins to the TPP/DIAD mixture. The reaction was stirred for a further Ihr at 0°C, then overnight at RT. The solvents were removed in vacuo to give a viscous pale yellow oil (ll.lg). The crude oil was purified over silica gel (250g) eluting with a gradient of heptane : ethyl acetate 4:1 -> 1:1. Product fractions were identified and reduced in vacuo to give (2S,3R) (3-formyloxy)pyrrolidine-l32-dicarboxylic acid 2-allyl ester l-(9H-fluoren-9-ylmethyl) ester / Bis-hydrazide of DIAD, a white solid, yield 4.0g. TLC (single UV spot, Rf = 0.50, heptane : ethyl acetate 1:1), analytical HPLC Rt = 19.064mins, HPLC-MS (single main UV peak with Rt = 9.721mins, 422.2 [M + H]+, 444.1 [M + Na]+ and a non-UV peak with Rt = 5.057mins, 205.1 [M + Hf, 431.2 [2M + Naf).
(4) Preparation of (2S,3R) (3-hydroxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl
ester l-(9H-fluoren-9-ylmethyl) ester.
(2S,3R) (3-formyloxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester 1-(9H-fluoren-9-ylmethyl) ester / Bis-hydrazide of DIAD mixture (4.0g) was dissolved in allyl alcohol (18mL), cH2SO4 (75p.L) added and heated to reflux
for 90mins. The mixture was cooled, EtOAc (200mL) added and washed with NaHCO3 (200mL), brine (200mL) and dried (Na2SO4). The solvents were removed in vacuo to give a viscous tan oil (4.2g). The crude oil was purified over silica gel (150g) eluting with a gradient of heptane : ethyl acetate 2:1 -> 1:1. Desired fractions were combined and reduced in vacuo to a colourless gum yield 1.75g (4.44mmole, 42.4%). TLC (single UV spot, Rf = 0.25, heptane : ethyl acetate 1:1), analytical HPLC Rt = 17.795mins, HPLC-MS (single main UV peak with Rt = 8.574mins, 394.2 [M + Hf, 416.2 [M + Na]+). Analysis by !H and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond.
8H (CDC13 at 298K); 2.09-2.19 (2Hy, m), 2.52 (OH, dd, / 1.0, 6.0), 3.54-3.65/3.70-3.82 (2H5, m), 4.14-4.20/4.22-4.30 (Fmoc H-9, dm), 4.30-4.39 (1 x Fmoc CH2, m), 4.43-4.58 (Ha + 1 x Fmoc CH2, m), 4.60-4.65 (Hp, m) 4.65-4.76 (2 x COOCH2CH=CH2, m), 5.23-5.36 (2 x COOCH2CH=CH2, m), 5.81-6.00 (1 x COOCH2CH=CH2, m), 7.28-7.38 (2H aromatic, Fmoc H-2 and H-7), 7.39-7.46 (2H aromatic, Fmoc H-3 and H-6), 7.53-7.69 (2H aromatic, Fmoc H-l and H-8), 7.73-7.81 (2H aromatic, Fmoc H-4 and H-5). 5C (CDC13 at 298K); 32.34/33.22 (d, Cy), 44.65/44.90 (d, C5), 47.57/47.63 (u, Fmoc C-9), 63.89/64.22 (u, Ca), 66.38/66.41 (d, COOCH2CH=CH2), 68.02 (d, Fmoc CH2), 71.84/72.80 (u, Cp), 119.08/119.31 (d, COOCH2CH=CH2), 120.39 (u, Fmoc C-4 and C-5), 125.32/125.46/125.56 (u, Fmoc C-l and C-8), 127.46 (u, Fmoc C-2 and C-7), 128.12 (u, Fmoc C-3 and C-6), 132.03/132.14 (u, COOCH2CH=CH2), 141.65/141.69 (q, Fmoc C-4' and C-5'), 144.00/144.16/144.43/144.48 (q, Fmoc C-l' and C-8'), 154.88/155.25 (q, OCON), 169.94/169.99 (COOCH2CH=CH2).
(5) Preparation of (2S,2>R) (3-fe7^butoxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester 1 -(9H-fluoren-9-ylmethyl) ester.
(2S,3K) (3-hydroxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester 1-(9H-fluoren-9-ylmethyl) ester (1.75g, 4.45mmole) was dissolved in dry dichloromethane (20mL) in a 50mL glass pressure tube and cooled to -78°C. Isobutylene gas (~10mL) was condensed into the solution and cH2SO4
(lOOuL) added. A stirrer bar was added, the tube was sealed, the cooling removed and stirred at RT for 72hr. The sealed tube was cooled to -78°C, N-methylmorpholine (200|iL, leq w.r.t. cHaSC^) and allowed to warm to RT, unsealed, with stirring over 2hr. Dichloromethane (75mL) was added and the organics washed with NaHCO3 (75mL), then brine (75mL) and dried (Na2SO4). The solvents were removed in vacuo to give a mobile pale tan oil (2.41 g). The crude oil was purified over silica gel (150g) eluting with a gradient of heptane : ethyl acetate 4:1 -> 3:1. Desired fractions were combined and reduced in vacuo to a thick clear oil yield 1.66g (3.69mmole, 83.0%). TLC (single UV spot, Rf = 0.45, heptane : ethyl acetate 2:1), analytical HPLC Rt = 22.81 Imins, HPLC-MS (single main UV peak with Rt = 11.193mins, 450.2 [M + H]+, 472.2 [M + Naf, 921.4 [2M + Naf).
(6) Preparation of (2S,3>K) (3-te^butoxy)pyrrolidine-l,2-dicarboxylic acid 1-(9H-fluoren-9-ylmethyl) ester.
(2S,3R) (3-/er/-butoxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester 1-(9H-fluoren-9-ylmethyl) ester (1.60g, 3.56mmole) was dissolved in dry dichloromethane (20mL) with stirring. Tetrakistriphenylphosphine palladium (0) (83mg, 0.071mmole, 0.02eq) was added, followed by phenyltrihydrosilane (0.77g, 0.674mL, 7.12mmole, 2eq). After 2hr, dichloromethane (150mL) was added and the organics washed with 0.01N HC1 (150mL), brine (150mL) and dried (Na2SO4). The solvents were removed in vacuo to give a dark grey solid (2.14g). The crude solid was purified over silica gel (75g) eluting with a gradient of heptane : ethyl acetate 2:1 -^ 1:6. Desired fractions were combined and reduced in vacuo to a white crystalline yield 0.89g (2.17mmole, 61.1%). TLC (single UV spot, Rf = 0.30, heptane : ethyl acetate 1:2), analytical HPLC Rt = 19.135mins, HPLC-MS (single main UV peak with Rt = 9.386mins, 354.1 [M + H-Bu'f, 410.2 [M + H]+, 432.1 [M + Naf, 841.1 [2M + Naf). Analysis by 'H and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond.
8H (CDC13 at 298K); 1.23 (9H, s), 1.98-2.28 (2HY, m), 3.40-3.50 (1H6, m), 3.68-3.81 (IHfi, m), 4.10-4.50 (Ha + Hp + Fmoc H-9 + 2 x Fmoc CH2, m), 7.29-7.40 (2H aromatic, Fmoc H-2 and H-7), 7.42-7.47 (2H aromatic, Fmoc H-3 and H-6), 7.55-7.70 (2H aromatic, Fmoc H-1 and H-8), 7.73-7.85 (2H aromatic, Fmoc H-4 and H-5), 6.30-8.30 (COOH, b). 5C (CDC13 at 298K); 28.285 (u, (CH3)C), 31.47/32.29 (d, CJ, 44.13/44.38 (d, C5), 47.51/47.60 (u, Fmoc C-9), 62.73/62.95 (u, Ca), 67.96/68.13 (d, Fmoc CH2), 71.77/72.67 (u, Cp), 120.33 (u, Fmoc C-4 and C-5), 125.39 /125.50/125.55/125.61 (u, Fmoc C-l and C-8), 127.46 (u, Fmoc C-2 and C-7), 128.01/128.07 (u, Fmoc C-3 and C-6), 141.58/141.66 (q, Fmoc C-4' and C-5'), 144.04/144.23/144.46 (q, Fmoc C-l' and C-8'), 154.95/155.32 (q, OCON), 175.19/175.65 (COOH).
(7) Preparation of (2S,3K) (3-te7^butoxy)-2-(2-diazoacetyl)pyrrolidine-l-carboxylic acid 9#-fluoren-9-ylmethyl ester.
(2S,3R) (3-ter^butoxy)pyrrolidine-ls2-dicarboxylic acid l-(9H-fluoren-9-ylmethyl) ester (615mg, 1.5mmole) was dissolved with stirring in dry dichloromethane (15mL). The reaction was flushed with nitrogen and cooled to -15°C. Isobutylchloroformate (225mg, 1.6mmole in dry dichloromethane (2.5mL) and N-methylmorpholine (303mg, 3.0mmole in dry dichloromethane (2.5mL) were added simultaneously in 0.5mL aliquots over ISmins. Etheral diazomethane (generated from diazald (4.7g, ~15mmole in diethyl ether (75mL)) onto sodium hydroxide (5.25g) in water (7.5mL) / ethanol (15mL) at 60°C). was added to the activated aminoacid solution and stirred at RT for 24hr. Acetic acid (~2mL) was added to quench the reaction, then tert-butylmemylether (150mL) was added, the organics washed with water (3 x 200mL), then dried (Na2SO4). The solvents were removed in vacuo to give a thick yellow oil (890g). The crude oil was purified over silica gel (75g) eluting with a gradient of heptane : ethyl acetate 3:1 -> 2:1. Desired fractions were combined and reduced in vacuo to give (2S,3R) (3-fe;t-butoxy)-2-(2-diazoacetyl)pyrrolidine-l-carboxylic acid 9#-fiuoren-9-ylrnethyl ester, a pale yellow solid, yield 270mg (0.624mmole, 41.6%). TLC (single UV spot, Rf=
0.55, heptane : ethyl acetate 1:1), analytical HPLC Rt = 20.085mins, HPLC-MS (2 main UV peaks with Rt = 7.48mins, 350.1 [M + H]+, 372.1 [M + Naf, 721.0 [2M + Na]+ and Rt = 9.949mins, 406.2 [M + H-N2f, 456.2 [M + Naf, 889.3 [2M + Naf).
Note that upon HPLC-MS analysis, m/z 350.1 corresponds to the desired bicycle product (3aS, 6aR) 3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carboxylic acid 9#-fluoren-9-ylmethyl ester (19).
(8) Cyclisation to (3aS, 6aK) 3-oxo-hexahydrofuro[3,2-&]pyrrole-4-carboxylic acid 9#-fluoren-9-ylmethyl ester (19).
A solution of lithium chloride (205mg, 4.8mmole) in water (1.25mL) and acetic acid (5.0mL) was added to (2S,3£) (3-te/*-butoxy)-2-(2-diazoacetyl)pyrrolidine-l-carboxylic acid 9H-fluoren-9-ylmethyl ester (209mg, 0.483mmole). Gas was evolved and the yellow oily solid dissolved over Ihr to give a virtually colourless solution. After 90mins, chloroform (150mL) was added and the organics washed with NaHCOa (2 x ISOmL), brine (ISOmL) and dried (Na2SC>4). The solvents were removed in vacuo to give a thick yellow gum (200mg). The crude gum was purified over silica gel (40g) eluting with a gradient of heptane : ethyl acetate 3:2 -> 1:1. Desired fractions were combined and reduced in vacuo to a white crystalline solid, yield 133mg (0.38mmole, 18.6% from starting acid). TLC (single UV spot, Rf = 0.25, heptane : ethyl acetate 1:1), analytical HPLC broad peak Rt = 15.2-17.6mins, HPLC-MS (single broad UV peak with Rt = 7.45-8.56mins, 350.1 [M + Hf, 372.1 [M + Naf, 721.2 [2M + Naf). Analysis by 1H and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond.
5H (CDC13 at 298K); 1.61-1.97 (2Hy, m), 3.32-3.45 (1H8, m), 3.66-3.75/3.85-3.95 (1/2H5+ 1/2H8, dm), 3.95/4.10 (1 x COCH2a + 1 x COCH2p, dm), 4.15-4.30 (Fmoc H-9 + 2 x Fmoc CH2, m), 4.40-4.60 / 4.80-4.92 (Ha + Hp, complex), 7.20-7.30 (2H aromatic, Fmoc H-2 and H-7), 7.31-7.42 (2H aromatic, Fmoc H-3 and H-6), 7.50-7.57/7.60-7.66 (2H aromatic, Fmoc H-l and H-8), 7.68-7.76 (2H aromatic, Fmoc H-4 and H-5).
32.28 (d, Cy), 45.59/45.95 (d, Cs), 47.64 (u, Fmoc C-9), 62.26/62.77 (u, Ca), 68.03/68.65 (d, Fmoc CH2), 71.28 (d, COCH2), 82.17/83.11 (u, Cp), 120.38 (u, Fmoc C-4 and C-5), 125.41 /125.59 /125.88 (u, Fmoc C-l and C-8), 127.45/127.49 (u, Fmoc C-2 and C-7), 128.13 (u, Fmoc C-3 and C-6), 141.73 (q, Fmoc C-4' and C-5'), 144.16/144/37/144.88 (q, Fmoc C-l' and C-8'), 155.33 (q, OCON), 209.32 (COCH2).
Following the general details from Scheme 2, the required bicycle building block (3a5} 6aK) 3-oxo-hexahydroruro[3,2-6]pyrrole-4-carboxylic acid 9-fiT-fluoren-9-ylmethyl ester (19) was converted to building block-linker construct (21) as follows:
(3aS, 6a#) 3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carboxylic acid 9#-fiuoren-9-ylmethyl ester (19) (lOOmg, 0.286rnmole) was dissolved in a mixture of ethanol (S.OmL) and water (0.75mL) containing sodium acetate.trihydrate (59mg, 0.428mmole, l.Seq). 4-[[(hydrazinocarbonyl)amino] methyl]cyclohexanecarboxylic acid, trifluoroacetate (95mg, 0.286mmole, 1 .Oeq, Murphy, A. M. et al, J. Am. Chem. Soc., 114, 3156-3157, 1992) was added and the mixture refluxed for 90mins. Chloroform (50mL) was added and the organics washed with HC1 (50mL, ~ pH3), dried (Na2SO4) and reduced in vacuo to provide crude building block-linker construct (21) as a colourless gum. Yield 172mg, analytical HPLC 2 peaks Rt = 16.477 and 18.512mins (cis / trans geometrical isomers), HPLC-MS (2 x UV peak with Rt = 7.75 and 8.82mins, 547.3 [M + H]+, 569.3 [M + Na]4). Crude (21) was used directly for construct loading.
Following the general details from Scheme 2, the required building block-linker construct (21) was attached to the solid phase providing loaded building block-linker construct (22) as follows:
Building block-linker construct (21) (0.26mmoles), 2-(lH-benzotriazole-l-yl)-1,1,3,3-tetramethyluroniumhexafluoro phosphate (HBTU, 98.6mg, 0.26mmole), 1-hydroxybenzorriazole.hydrate and (HOBT, 39.8mg, 0.26mmole) were dissolved in dimethylformamide (3mL) and N-methylmorpholine (NMM, 58^L, 0.52mmole)
added. After pre-activation for 5 minutes, free arnine gears (72 x 1.2umole) were added, followed by dimethylformamide (5.5mL) and left overnight. The spent coupling solution was then added to free amine crowns (10 x lOumole) and left overnight. Standard washing and analyses indicated quantitative loading.
Following the general details from Scheme 2, the required loaded building block-linker construct (22) was elaborated on the solid phase as follows:
Loaded construct (22) was elaborated to EXAMPLE 1 (3aS, 6aR) JV-[liS-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide by standard Fmoc deprotection and sequential coupling with Fmoc-Tyr(OBut)-OH then benzoic acid. The crude example was cleaved and analysed (see general techniques). HPLC Rt = 10.15mins (>95%), HPLC-MS 395.1 [M + Hf.
The following examples (2 - 82) were prepared as detailed for EXAMPLE 1, coupling with the required reagents to provide the full length molecule.
EXAMPLE 2. (2>aS, 6aR) Biphenyl-4-carboxylic acid [15-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
HPLC Rt = 14.94-15.46mins (> 90%), HPLC-MS 471.2 [M + H]+.
EXAMPLE 3. (3aS, 6aR) 4-re^-Butyl-JV-[l1Sr-(4-hydroxyben2yl)-2-oxo-2.(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]benzamide
HPLC Rt = 15.29-15.89mins (> 90%), HPLC-MS 451.3 [M + H]+
EXAMPLE 4. (3aS, 6aR) ^-[15-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyirol-4-yl)ethyl]-5-thiophen-2-ylnicotinamide
HPLC Rt = 11.12 mins (> 90%), HPLC-MS 478.2 [M + H]+
EXAMPLE 5. (3a5, 6aR) 2-Pyridin-3-ylthiazole-4-carboxylic acid [IS-^hydroxy ben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
HPLC Rt = 8.58mins (> 90%), HPLC-MS 479.2[M + H]+
EXAMPLE 6. (2>aS, 6aR) JV-[15-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexaliydrofuro [3,2-6]pyrrol-4-yl)ethyl]-4-thiophen-2-ylbenzamide
HPLC Rt = 14.77-15.3Imins (> 90%), HPLC-MS 477.2 [M + H]+
EXAMPLE 7. (3aS, 6aR) A'-[15r-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexaliydroftiro [3,2-6]pyrrol-4-yl)ethyl]-4-trifluoromthoxybenzamide
EXAMPLE 8. (3oS, 6aK) Biphenyl-4-carboxylic acid [3-methyl-15-(3-oxo-hexahydrofuro[3,2-&]pyrrole-4-carbonyl)butyl]amide
EXAMPLE 10. (3aS, 6aK) //-[3-methyl-l1S-(3-oxo-hexahydrofuro[3,2-&]pyrrole-4-rbonyl) butyl]-4-phenoxybenzamide5XAMPLE 11. (3aS, 6aK) JV-[3-methyl-15-(3-oxo-hexahydrofuro[3,2-fe]pyrrole-4-;arbonyl) butyl]-3-pherioxybenzamide
HPLC Rt = 17.34-18.52mins (> 90%), HPLC-MS 437.2 [M + Hf
EXAMPLE 12. (3aS, 6aR) 4-Bromo-AT-[3-methyl-15-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-cafbonyl) butyljbenzamide
HPLC Rt = 14.81mins (> 90%), HPLC-MS 423.2 / 425.2 [M + H]+
EXAMPLE 13. (3aS, 6aR) 3-Bromo-^-[3-methyl-15-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyljbenzamide
HPLC Rt = 14.85-16.20mins (> 90%), HPLC-MS 423.2 / 425.2 [M + H]+
EXAMPLE 14. (3aS, 6aR) 4-Isopropyl-A'-[3-methyl-15-(3-oxo-hexahydrofuro[3,2-&]pyrrole-4-carbonyl) butyl]benzamide
HPLC Rt = 16.41-17.65mins (> 90%), HPLC-MS 387.2 [M + Hf
EXAMPLE 15. (3aS, 6aR) W-[3-methyl-15-(3-oxo-hexahydroforo[3,2-6]pyrrole-4-carbonyl) butyl]-4-vinylbenzamide
HPLC Rt = 14.63mins (> 90%), HPLC-MS 371.1 [M + H]+
EXAMPLE 16. (3aS, 6aR) 4-Imidazol-l-yl-A'-[3-methyl-15-(3-oxo-hexahydrofuro [3,2-i]pyrrole-4-carbonyl) butyl]benzatnide
HPLC Rt = 9.20mins (> 90%), HPLC-MS 411.2 [M + H]+
EXAMPLE 17. (3oS, 6aR) //-[3-methyl-15-(3-oxo-hexahydrofuro[3,2-i]pyrrole-4-carbonyl)butyl]-4-thiophen-2-ylben2amide
HPLC Rt = 16.49-18.34mins (> 90%), HPLC-MS 427.2 [M + H]+
EXAMPLE 18. (3oS, 6aR) JV-[3-methyl-15-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl)butyl]-4-oxazol-5-ylbenzamide
HPLC Rt = 12.79mins (> 90%), HPLC-MS 412.2 [M + H]+
EXAMPLE 19. (3aS, 6aR) 2-Phenylthiazole-4-carboxylic acid [3-methyl-15-(3-oxo-
hexaliydrofuro[3,2-6]pyrrole-4-carbonyl) butyl] amide
HPLC Rt = 16.41-17.80mins (> 90%), HPLC-MS 428.2 [M + H]+
EXAMPLE 20. (3aS, 6aK) 5-Phenylthiophene-2-carboxylic acid [3-methyl-15-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl)butyl]amide
HPLC Rt = 16.89mins (> 90%), HPLC-MS 427.2 [M + H]+
EXAMPLE 21. (3>aS, 6aR~) Quinoline-6-carboxylic acid [3-methyl-l,S-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl)butyl]amide
HPLC Rt = 9.08mins (> 90%), HPLC-MS 396.2 [M + Hf
EXAMPLE 22. (3aS, 6aR) JV-[3-methyl-l1Sr-(3-oxo-hexahydrofiiro[3,2-6]pyrrole-4-carbonyl) butyl]-4-[l ,2,3]thiadiazol-
HPLC Rt = 13.41mins (> 90%), HPLC-MS 429.2 [M + H]+
EXAMPLE 23. (3aS, 6aR) ^-[3-methyl-15-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl)butyl]-5-thiophen-2-ylnicotinamide
HPLC Rt = 13.24mins (> 90%), HPLC-MS 428.2 [M + H]+
EXAMPLE 24. (3aS, 6aR) 2-Pyridin-3-ylthiazole-4-carboxylic acid [3-methyl-15-(3-oxo-hexahydrofaro[3,2-6]pyrrole-4-carbonyl)butyl]amide
(Figure Remove) HPLC Rt = 10.22mins (> 90%), HPLC-MS 429.2 [M + Hf EXAMPLE 25. (3aS, 6aK) JV-tS-methyl-l^-C carbonyl)butyl]-4-trifluoromethoxybenzamide
HPLC Rt = 16.90-18.34mins (> 90%), HPLC-MS 429.2 [M + Hf
EXAMPLE 26. (3aS, 6aK) 4-fe7t-Butyl-AT-[l1S-(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-b]pyrrol-4-yl)ethyl]benzamide
HPLC Rt = 16.14-17.02mins (> 90%), HPLC-MS 451.2 [M + Hf
EXAMPLE 27. (3aS, 6aK) JV-[15-(3-hydroxybenzyl)-2-oxo-2-(3-oxo-hexaliydrofuro [3r2-6]pyrrol-4-yl)ethyl]-4-thiophen-2-ylbenzamide(Figure Remove)
HPLC Rt = 15.31-16.07mins (> 90%), HPLC-MS 477.2 [M + Hf
EXAMPLE 28. (3aS, 6aK) 4-te^-Butyl-^-[15-(4-fluorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-b]pyirol-4-yl)ethyl]ben2amide
HPLC Rt = 18.37-19.63mins (> 90%), HPLC-MS 453.1 [M + H]+
EXAMPLE 29. (3aS, 6aK) ]V-[l1S-(4-fluorobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]-4-thiophen.-2-ylbenzamide
HPLC Rt = 17.45-18.59mins (> 90%), HPLC-MS 479.2 [M + Hf
EXAMPLE 30. (3aS, 6aR) Morpholine-4-carboxylic acid [lS-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
HPLC Rt = 7.38mins (>95%), HPLC-MS 404.2 [M + H]+.
EXAMPLE 31. (3aS, 6aK) Morpholine-4-carboxylic acid [3-methyl-15-(3-oxo-hexa h.ydrofuro[3.,2-6]pyrrole-4-carbonyl)biityl]amide
HPLC-MS 354.2 [M + Hf.
EXAMPLE 32. (3aS, 6aR) Morpholine-4-carboxylic acid [lS-cyclohexylmethyl-2-
oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
HPLC-MS 394.2 [M + Hf.
EXAMPLE 33. (3aS, 6aK) Morpholine-4-carboxylic acid [15-benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-/>]pyrrol-4-yl)ethyl]amide
HPLC Rt = 10.07mins (>95%), HPLC-MS 388.1 [M + H]+.
EXAMPLE 34. (2RS, 3aS, 6aK) 4-(2-Benzyloxy-3-[cyclohexyl]propionoyl)tetra hydrofuro [3,2-£]pyrrol-3-one.
HPLC Rt = 17.5-19.7mins (>90%), HPLC-MS 372.4 [M + H]+, 390.3 [M + H + 18]+.
(a) Preparation of 3-Cyclohexyl-2S-hydroxypropionic acid (Compound (25) Scheme 4)
A solution of sodium nitrite (12.1g, 175mmol) in water (40ml) was added dropwise to a stirred suspension of (S)-a-aminocyclohexanepropionic acid hydrate (5g, 26.5mmol) in 0.5M sulphuric acid (120ml, 60mmol) at 0°C over 1.Shouts. The mixture was allowed to warm to ambient temperature over 20hours. The product was extracted into diethyl ether (2 x 25ml) then the ethereal layers were washed with saturated aqueous sodium chloride solution (2 x 25ml), dried (Na2S04) and the solvents removed in vacua. The residue (5.3g) was recrystallized from diethyl ether (10ml) and heptane (25ml) to give 3-cyclohexyl-2S-hydroxypropionic acid as a white solid, yield 2.4 g, (53%).
5H (400MHz, CDC13 at 298K), 0.89-1.35 (5H, m) and 1.51-1.86 (7H, m) (OCHCH2 and cyclohexyl), 4.32 (1H, OCHCH2, m)
(b)Preparation of 2&S'-Benzyloxy-3-cyclohexylpropionic acid (Compound (26) Scheme 4)
Sodium hydride (265mg of 60% dispersion in oil, 6.6mmol) was added in two portions to a stirred mixture of S-cyclohexyWS'-hydroxypropionic acid (0.52g, S.Ommol), dimethylformamide (5ml) and dichloromethane (5ml) at 0°C over Sminutes. The mixture was stirred at 0°C for Sminutes then at ambient temperature for 45minutes. Benzyl bromide (0.45ml, 3.8mmol) was added then the mixture stirred for 1 hour before adding dimethylformamide (5ml). After stirring for 4 hours potassium iodide (50mg, 0.3mmol) was added. The mixture was stirred for 20hours then heated at 55°C for Ihour then allowed to cool to ambient temperature and poured into water (15ml). A saturated aqueous sodium chloride solution (5ml) was added then the mixture was extracted with dichloromethane (5ml then 10ml) that was discarded. The aqueous layer was acidified using 1M hydrochloric acid (10 ml) then
extracted with dichloromethane (2 x 10ml). The dichloromethane layer was dried (MgSCU) and the solvent removed in vacua. The residue (0.5 5g) was dissolved in dimethylformamide (8ml) then cooled to 0°C before adding sodium hydride (190mg of 60% dispersion in oil, 4.75mmol). The mixture was stirred for 30 minutes then polymer bound isocyanate (380mg, 2mmolNg~1) added. The mixture was stirred for 2 hours at ambient temperature then poured into water (15ml). 1M Hydrochloric acid (10ml) was added then the product was extracted into dichloromethane (2 x 10ml), dried (Na2SC>4) and the solvent removed in vacua. The residue was purified by flash chromatography over silica gel eluting with a gradient of methanol: dichloromethane 0:1 -> 1:20. Appropriate fractions were combined and the solvents removed in vacua to give 2.&Sr-benzyloxy-3-cyclohexylpropionic acid as a colourless oil, yield 41mg (5.2%).
HPLC-MS (single main UV peak with Rt = 9.47mins, 261.2 [M - H]", 285.2[M + Naf,547.3[2M + Na]+).
5H (400MHz, CDC13 at 298K), 0.72-1.03 (2H, cyclohexane, m), 1.08-1.38 (3H, cyclohexane, m), 1.45-1.93 (6H + 2Hp, cyclohexane, m), 3.93-4.18 (IHa, OCHCO), 4.35-4.53 (1H, CH2O, d, /= 11.52 Hz), 4.68-4.88 (1H CH2O, d, J= 11.54 Hz), 7.20-7.47 (5H, ArH, m), 9.36 (1H, OH, brs).
Compound (26) was coupled under standard conditions to loaded building block-linker construct (22), then cleaved to provide EXAMPLE 34.
EXAMPLE 35. (3aS, 6aK) Morpholine-4-carboxylic acid [17?-benzylsulfanylmethyl-2-oxo-2-(3 -oxo-hexahydrofuro[3,2-i]pyrrol-4-yl)ethyl]amide
HPLC Rt = 12.15mins (>95%), HPLC-MS 44.1 [M + Hf.
EXAMPLE 36. (3aS, 6aR) ^-[3-methyl-15-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyljbenzamide
HPLC Rt = 12.43mins (>95%), HPLC-MS 345.2 [M + H]+.
HPLC Rt = 15.09mins (>95%), HPLC-MS 425.1 [M + Hf.
EXAMPLE 37. (3aS, 6aR) JV-[l^-benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydro furo[3,2-&]pyrrol-4-yl)ethyl]benzamide
EXAMPLE 38. (3aS, 6aK) AT-[l,S'-cyclohexylmethyl-2-oxo-2-(3-oxo-hexaliydrofuro [3,2-6]pyrrol-4-yl)ethyl]benzarnide
HPLC Rt = 15.63mins (>90%), HPLC-MS 385.3 [M + H]+, 791.3 [2M + Naf.
EXAMPLE 39. (3aS, 6aK) N-[lS-benzyl-2-oxo-2-(3-oxo-hexahydrofaro[3,2-6]pyrrol-4-yl)ethyl]benzarnide
HPLC Rt = 12.85mins (>90%), HPLC-MS 379.1 [M + H]+.
EXAMPLE 40. (3aS, 6aR) Furan-3-carboxylic acid [15-cyclopentylmethyl-2-oxo-2-(3-oxo-hexah.ydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
HPLC Rt = 13.23mins (>95%), HPLC-MS 361.2 [M + H]+.
EXAMPLE 41. (3oS, 6aK) 7V-[15-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-6]pyrrol-4-yl)ethyl]-2-phenylacetamide
HPLC Rt = lOJlmins (>95%), HPLC-MS 409.1 [M + H]+.
EXAMPLE 42. (3aS, 6aR) ^[3-me1hyl-15-(3K>xo-hexahydrofuro[3^-6]pyirole-4-carbonyl) butyl]-2-phenylacetamide
HPLC Rt = 13.00mins (>95%), HPLC-MS 359.2 [M + H]+.
EXAMPLE 43. (3aS, 6aR) JV-[l/?-benzylsulfanylmethyl-2-oxo-2-(3-oxo-b.exahydro furo[3}2-6]pyrrol-4-yl)ethyl]-2-phenylacetamide
(Figure Remove)HPLC Rt = 15.53mins (>90%), HPLC-MS 439.1 [M + H]+.
EXAMPLE 44. (3aS, 6aR) J?/-[15r-cyclohexylmethyl-2-oxo-2-(3-oxo-hexahydrofuro [3,2-fe]pyrrol-4-yl)ethyl]-2-phenylacetamide
HPLC Rt = 18.5mins (>90%), HPLC-MS 399.2 [M + H]+.
EXAMPLE 45. (3aS, 6aK) JV-[lJSr-ben2yl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]-2-phenylacetamide
HPLC Rt = 13.2mins (>90%), HPLC-MS 393.2 [M + H]+.
EXAMPLE 46. (3aS, 6aK) Furan-3-carboxylic acid [16'-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexab.ydrofuro[3,2-Z>]pyrrol-4-yl)ethyl]ainide
HPLC Rt = 8.65mins (>95%), HPLC-MS 385.2 [M + Hf.
EXAMPLE 47. (3aS, 6aK) Furan-3-carboxylic acid [3-metliyl-15'-(3-oxo-hexahydro ruro[3,2-fo]pyrrole-4-carbonyl)butyl]amide
HPLC Rt = 10.95mins (>90%), HPLC-MS 335.1 [M + H]+, 357.1 [M + Naf.
EXAMPLE 48. (3aS, 6aR) Furan-3-carboxylic acid [l^-ben2ylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)ethyl]amide
LC Rt = 13.68mins (>90%), HPLC-MS 415.1 [M + Hf.
EXAMPLE 49. (3aS, 6aK) Furan-3-carboxylic acid [15'-cycloriexylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
(Figure Remove)
HPLC Rt = 14.39mins (>90%), HPLC-MS 375.2 [M + H]+, 771.3 [2M + Na]+.
EXAMPLE 50. (3aS, 6aK) Furan-3-carboxylic acid [lS-ben2yl-2-oxo-2-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
HPLC Rt = 11 .Slmins (>90%), HPLC-MS 369.1 [M + Hf.
EXAMPLE 51. (3aS, 6aR) Thiophene-3-carb.oxylic acid [15-cyclopentyhnethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)et;hyl]arnide
HPLC Rt = 14.07mins (>95%), HPLC-MS 377.3 [M + Hf.
EXAMPLE 52. (3aS, 6aK) Thiophene-3-carboxylic acid [lS-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide

HPLC Rt = 11.75mins (>95%), HPLC-MS 401.3 [M + H]+.
EXAMPLE 53. (3aS, 6aR) Thiophene-3-carboxylic acid [3-methyl-15-(3-oxo-hexa hydroruro[3,2-fo]pyrrole-4-carbonyl)butyl]amide
HPLC Rt = H.Olmins (>90%), HPLC-MS 351.1 [M + H]+, 373.1 [M + Naf.
EXAMPLE 54. (3aS, 6aR) Thiophene-3-carboxylic acid [1^-benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
HPLC Rt = 14.51mins (>95%), HPLC-MS 431.1 [M + H]+.
EXAMPLE 55. (3aS, 6aR) Thiophene-3-carboxylic acid [15-cyclohexylmethyl-2-oxo-2-(3-oxo-hexahydroforo[3,2-6]pyrrol-4-yl)ethyl]amide
(Figure Remove)-157-1 [M + Hf, 803.3 [2M + Naf.
EXAMPLE 56. (3aS, 6aK) Thiophene-3-carboxylic acid [l,S-benzyl-2-oxc-2-(3-oxo-hexahydrofuro[3,2-&]pyrrol-4-yl)etb.yl]amide
HPLC Rt = 12.27mins (>95%), HPLC-MS 385.1[M + Hf, 407.1 [M + Naf, 791.2 [2M + Naf.
EXAMPLE 57. (3aS, 6aK) Furan-3-carboxylic acid [3,3-dimethyl-15-(3-oxo-hexa hydrofuro[3,2-6]pyrrole-4-carbonyl)butyl]amide
HPLC Rt = 12.50mins (>95%), HPLC-MS 349.2 [M + H]+.
EXAMPLE 58. (3aS, 6aR) JV-[15-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-Z)]pyrrol-4-yl)ethyl]-3-plienylpropionamide
HPLC Rt = 11.72mins (>90%), HPLC-MS 423.2 [M + H]+.
EXAMPLE 59. (3aS, 6aR) JV-[3-methyl-15r-(3-oxo-hexaliydrofuro[3,2-6]pyrrole-4-carbonyl) butyl] -3 -phenylpropionamide
HPLC Rt = 13.46mins (>90%), HPLC-MS 373.1 [M + H]+, 767.3 [2M + Naf.
EXAMPLE 60. (3aS, 6aK) JV-[l^-benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydro furo[3,2-6]pyrrol-4-yl)ethyl]-3-phenylpropionainide
HPLC Rt = 16.22mins (>90%), HPLC-MS 453.2 [M + H]+, 475.0 [M + Naf.
EXAMPLE 61. (3aS, 6aK) JV-[15-cycloliexylmethyl-2-oxo-2-(3-oxo-hexaliydrofuro [3,2-&]pyrrol-4-yl)ethyl]-3 -phenylpropionamide
(Figure Remove)
HPLC-MS 413.2 [M + Hf.
EXAMPLE 62. (3aS, 6aK) ^-[15-benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-b]pyrrol-4-yl)ethyl]-3-phenylpropionamide
HPLC Rt = 16.68mins (>90%), HPLC-MS 407.2 [M + Hf, 835.2 [2M + Naf.
EXAMPLE 63. (3aS, 6aK) Thiophene-3-carboxylic acid [3,3-dimethyl-l.S-(3-oxo-hexahydrofuro[352-6]pyrrole-4-carbonyl)butyl]amide
HPLC Rt = 13.24mins (>95%), HPLC-MS 365.2 [M + Hf.
EXAMPLE 64. (3aS, 6aR) Benzo[&]thiophene-2-carboxylic acid [15-(4-hydroxy ben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
HPLC-MS 451.1 [M + Hf, 923.2 [2M + Naf.
EXAMPLE 65. (3aS, 6aR) Benzo[6]thiopliene-2-carboxylic acid [3-methyl-l,Sr-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl)butyl]amide
HPLC Rt = 15.62mins (>90%), HPLC-MS 401.1 [M + Hf, 823.2 [2M + Naf.
EXAMPLE 66. (3aS, 6aR) Benzo[fo]thiophene-2-carboxylic acid [Ifl-beuzylsulfanyl methyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-i]pyrrol-4-yl)etiiyl]amide
HPLC Rt = 17.47mins (>90%)5 HPLC-MS 481.1 [M + Hf, 983.2 [2M +Naf.
EXAMPLE 67. (3aS, 6aK) Benzo[6]thiopheae-2-carboxylic acid [15-cyclohexyl methyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
HPLC Rt = 18.15mins (>90%), HPLC-MS 441.2 [M + Hf, 903.2 [2M + Naf.
EXAMPLE 68. (3aS, 6aR) Benzo[fc]thiophene-2-carboxylic acid [lS-benzyl-2-oxo-2-(3-oxo-hexab.ydrofuro[3,2-fe]pyrrol-4-yl)ethyl]ainide
HPLC Rt = 15.58mins (>85%), HPLC-MS 435.1 [M + H]+, 891.2 [2M + Naf.
EXAMPLE 69. (3aS, 6aK) Naphthalene-1-carboxylic acid [15'-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyrrol-4-yl)ethyl]amide
LC Rt = 12.42mins (>85%), HPLC-MS 445.1 [M + Hf, 911.3 [2M + Naf.
EXAMPLE 70. (3aS, 6aR) Naphthalene-1-carboxylic acid [3-methyl-lS-(3-oxo-hexa hydrofuro [3,2-6]pyrrole-4-carbonyl) butyl] amide
HPLC Rt = 14.81mins (>S5%), HPLC-MS 395.2 [M + Hf, 811.3 [2M + Na]+.
EXAMPLE 71. (3>aS, 6aR) Naphthalene-1-carboxylic acid [IJ^benzylsulfanylmethyl -2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
HPLC Rt = 17.10mins (>90%), HPLC-MS 475.2 [M + H]+.
EXAMPLE 72. (3aS, 6aR) Naphthalene-1-carboxylic acid [15-cyclohexyhnethyl-2-oxo-2-(3-oxo-hexah.ydrofuro[3,2-6]pyrrol-4-yl)ethyl]amide
HPLC Rt = 17.45mins (>90%), HPLC-MS 435.2 [M + H]+, 891.4 [2M + Naf.
EXAMPLE 73. (3aS, 6aR) Naphthalene-1-carboxyHc acid [15-benzyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-Z?]pyrrol-4-yl)ethyl] amide
HPLC Rt = 15.17miBs (>90%), HPLC-MS 429.1 [M + Hf, 879.3 [2M + Na]+.
EXAMPLE 74. (IS, 3aS, 6aR) Morpholine-4-carboxylic acid l-cyclohexylmethyl-2-oxo-2-(3-oxo-hexahydromro[3,2-&]pyrrol-4-yl)ethyl ester

HPLC-MS 395.2 [M + Hf, 811.4 [2M + Naf.
(a) Preparation of 3-cyclohexyl-2S-hydroxypropionic acid methyl ester. (Compound (32), Scheme 6)
Trimethylsilyl chloride (1.78ml, 14.0mmol) was added dropwise to a stirred solution of 3-cyclohexyl-2S-hydroxypropionic acid (compound (25) 1.31g, 7.6mmol) in methanol (35ml). The mixture was stirred at room temperature for 20hours then the solvents were removed in vacua to leave a pale yellow oil (1.38g). The crude oil was purified by chromatography over silica gel eluting with a gradient of n-heptane : ethyl acetate (4:1) Appropriate fractions were combined and the solvents removed in vacua to give 3-cyclohexyl-2S-hydroxypropionic acid methyl ester as a colourless oil, yield 1.26 g, (89%). HPLC-MS (single main peak with Rt = 7.43mins, 187.14 [M + Hf, 395.2 [2M +Naf).
6H (400MHz, CDC13 at298K), 0.84-1.04 (2H3 CH2(cyclohexane), m), 1.09-1.36 (3H, CH2(cyclohexane), CH (cyclohexane), m), 1.45-1.77 (7H CH2(cyclohexane), CH(cyclohexane), m), 1.80-1.90 (IHp, m), 2.72 (1H, OH, d, J= 5.99 Hz), 3.80 (3H, CH3O, s), 4.20-4.31 (IHa, m).
(b)Preparation of morpholine-4-carboxylic acid l-carboxy-2-cyclohexylethyl ester (Compound (33), Scheme 6)
A solution of phosgene (33.6ml, 20% in toluene) was added to 3-cyclohexyl-25r-hydroxypropionic acid methyl ester (compound (32), 0.7 g, 4.03mmol) followed by 6 drops of dimethylformamide. The mixture was stirred at ambient temperature for 16hours then the solvents removed in vacua. The residue was azeotroped with toluene (3 x 20ml), and dissolved in anhydrous dichloromethane (llml). The solution was cooled to 0°C then morpholine (0.86g, 9.85mmol) added. The mixture was stirred for 2 hours then partitioned between dichloromethane (30ml) and 0.5M hydrochloric acid (30ml). The dichloromethane layer was washed with saturated aqueous sodium hydrogen carbonate solution (30ml), saturated aqueous sodium chloride solution (30ml), dried (NaiSC^) and the solvent removed in vacua. The residue was purified by chromatography over silica gel eluting with ethyl acetate : heptane 1:1. Appropriate fractions were combined and the solvents removed in vacua to leave morpholine-4-cafboxylic acid 2-cyclohexyl-l-methoxycarbonylethyl ester (0.13g, 10%) as an oil. A solution of lithium hydroxide monohydrate (17.5mg, 0.418mmol) in water (0.76ml) was added to an iced-water chilled solution of morpholine-4-carboxylic acid 2-cyclohexyl-l-methoxycarbonylethyl ester (llOmg, 0.367mmol) in dioxane (1.5ml). The mixture was stirred at ambient temperature for Ihour then diluted with water (10ml). The aqueous layer was extracted with diethyl ether (2 x 10ml) which was discarded, men acidified to pH = 2 with 6M hydrochloric acid. The product was extracted into diethyl ether (2 x 10ml), then the combined ethereal layers washed with saturated aqueous sodium chloride (10ml), dried (MgS04) and the solvent removed in vacua to give morpholine-4-carboxylic acid 1-carboxy-25-cyclohexylethyl ester as a white solid, yield O.llg, (100% from ester). TLC (single spot, Rf = 0.20, methanol : dichloromethane 1:9), HPLC-MS (single main peak with Rt = 7.614mins, 286.2 [M + Hf, 287.2 [M + 2Hf, 593.3 [2M + Naf).
H (400MHz, CDC13 at 298K), 0.75-1.00 (2H, CH2(cyclohexane), m), 1.02-1.28 (4H, CH2(cyclohexane),m), 1.33-1.46 (1H, CH(cyclohexane), m), 1.50-1.79 (6H, CH2(cyclohexane), m), 3.28-3.73 (8H, CH2OCH2 and CH2NCH2 ,m), 4.92-5.02 (lHa,m),5.99(lH,OH,brs).
Compound (33) was coupled under standard conditions to loaded building block-linker construct (22), then cleaved to provide EXAMPLE 74.
EXAMPLE 75. (3aS, 6aR) 4-[2-(4-fer^-butylbenzylsulfanyl)-4-methylpentanoyl] tetrahydrofuro[3,2-6]pyrrol-3-one
HPLC Rt = 21.3-23.4mins (>80%), HPLC-MS 404.4 [M + H]+.
(a) Preparation of 2/2-Bromo-4-methylpentanoic acid (Compound (28), Scheme 5)
A solution of sodium nitrite (S.lg, 73mmol) in water (15ml) was added drop-wise at 0°C over Shours to a stirred mixture of D-leucine (8.75g, 67mmol), potassium bromide (29.75g, 0.25mol) and concentrated sulphuric acid (8.6ml) in water (100ml). The mixture was stirred for 30 minutes at 0°C then at ambient temperature for 20hours. The product was extracted into diethyl ether (2 x 150ml) then the combined ethereal layers were washed with saturated aqueous sodium chloride solution (2 x 100ml), dried (MgSO4) and the solvent removed in vacuo. The residue was purified by flash chromatography over silica gel eluting with a gradient of methanol : dichloromethane 1:50 -> 1:20. Appropriate fractions were combined and the solvents removed in vacuo to leave 2/?-bromo-4-methylpentanoic acid (28) as a colourless oil,
le spot, Rf= 0.2, methanol: dichloromethane 1:20). Additionally, a second crop (5.2g, 40%) of slightly impure product was obtained
5H (400MHz, CDC13 at 298K), 0.95 and 0.99 (both 3H, CH3CH, d, J = 6.55Hz). 1.77-1.89 (1H, CH3CH, m), 1.93 (2Hp, m), 4.31 (IHa, t, J= 7.7Hz), 9.3 (1H, CO2H, brs).
(b)Preparation of 2,S-(4-tert-butylbenzylsulfanyl)-4-methylpentanoic acid (Compound (30), Scheme 5)
A solution of 2J?-bromo-4-methylpentanoic acid (compound (28), l.lg, 5.6mmol) and (4-(fert-butyl)phenyl)methanethiol (l.Og, 5.6mmol) in dimethylformamide (15ml) was purged with nitrogen for 5 minutes then cooled to 0°C. Triethylamine (0.79ml, 5.7mmol) was added drop-wise over 1 minute then the mixture was stirred for two days at ambient temperature. The solvents were removed in vacua and residue purified by flash chromatography over silica gel eluting with a gradient of methanol : dichloromethane 0:1 -> 1:20. Appropriate fractions were combined and the solvents removed in vacua to leave a residue which was purified by flash chromatography over silica gel eluting with ethyl acetate : heptane 2:5. Appropriate fractions were combined and the solvents removed in vacua to give 2S-(4-tert-butylbenzylsulfanyl)-4-methylpentanoic acid (30) as a colourless oil, yield 150 mg, (9%). TLC (single spot, Rf = 0.2, heptane : ethyl acetate 5:2), analytical HPLC with main peak Rt = 22.117mins, HPLC-MS (main UV peak with Rt = 11.072mins, 317.2 [M + Naf).
oH (400MHz, CDC13 at 298K), 0.70 and 0.85 (both 3H, CH3CH, d, / = 6.3), 1.29 (9H, (CH3)3C, s), 1.44-1.51 (1H, CH3CH, m), 1.62-1.75 (2H(3, m), 3.15-3.20 (IHa, m), 3.81 and 3.88 (both 1H, SCH2, d, J- 13.2Hz), 7.25-7.35 (4H, aromatic).
Compound (30) was coupled under standard conditions to loaded building block-linker construct (22), then cleaved to provide EXAMPLE 75.
EXAMPLE 76. (3aS, 6aK) 4-[3-Cyclohexyl-2S-(ruran-2-ylmethanesulphonyl) propionyl]tetrahy drofuro[3,2-6]pyrrol-3 -one
HPLC Rt = 12.8 - IS.lmins (>90%), HPLC-MS 410.1 [M + Hf, 841.2 [2M + Naf. (a)Preparation of (J?)-a-bromocyclohexanepropionic acid
A solution of sodium nitrite (0.45g, 6.5mmol) in water (1.2ml) was added dropwise at 0°C over 4 hours to a stirred mixture of (R)-a-aminocyclohexanepropionic acid (Ig, S.Smmol), potassium bromide (2.3g, 19.2mmol) and concentrated sulphuric acid (0.66ml) in water (7ml). The mixture was stirred for Shours at 0°C then at ambient temperature for 16hours. The product was extracted into diethyl ether (4 x 20ml) then the combined ethereal layers were washed with saturated aqueous sodium chloride solution (2 x 50ml), dried (MgSCU) and the solvent removed in vacua. The residue was purified by chromatography over silica gel eluting with a gradient of methanol: dichloromethane 1:50 -^ 1:20. Appropriate fractions were combined and the solvents removed in vacua to give (#)-a-bromocyclohexanepropionic acid as a pale yellow oil 0.374 g, (27%). TLC (single spot, Rf = 0.45, 10% methanol in dichloromethane), HPLC-MS (single main peak with Rt = 8.88mins, 234.1 / 236.1 [M + Hf, 257.2 / 259.2 [M + Naf).
5H (400MHz, CDC13 at 298K), 0.83-1.88 (11H, CH(cyclohexane), CH2 (cyclohexane), m), 1.89-2.05 (2H(3, m), 4.32-4.44 (IHa, m).

(b)Preparation of 3-cyclohexyl-25r-(furan-2-ylmethylsulfanyl)propionic acid (Compound (41), Scheme 9)
(#)-a-bromocyclohexanepropionic (0.37g, 1.58rnmol) and furan-2-yl methanethiol (0.18g, 1.58mmol) were dissolved in dimethylformamide (10ml) and purged with nitrogen for lOminutes. The solution was cooled to 0°C then triethylamine (0.22ml, 1.58mmol) was added drop-wise over 1 minute. The mixture was stirred at 0°C for SOminutes then at ambient temperature for 16hours. The solvent was removed in vacuo, and the residue purified by chromatography over gel silica using methanol : dichloromethane 1:100 as eluent. Appropriate fractions were combined and the solvents removed in vacuo to give 3-cyclohexyl-2iS-(furan-2-ylmethyl sulfanyl)propionic acid (41) as a light brown oil, yield 142mg, (33%). Analytical HPLC peak Rt = 18.68mins. TLC (single spot, Rf = 0.45, 10% methanol in dichloromethane), HPLC-MS (single main peak with Rt = 9.53mins, 291.0 [M + Naf).
6H (400MHz, CDC13 at 298K), 0.68-0.89 (2H, CH2(cyclohexane), m), 0.97-1.77 (11H, CH(cyclohexane), CH2(cyclohexane), m), 3.21-3.32 (IHgt, t, J= 7.79 Hz), 3.69-3.79 (1H, CH2S, d, J = 14.79 Hz), 3.84-3.94 (1H, CHaS, d, J= 14.8 Hz), 6.15-6.28 (2H, furan H-3 and H-4, d, J= 27.34 Hz), 7.30 (1H, furan H-5, s).
Compound (41) was coupled under standard conditions to loaded building block-linker construct (22). The intermediate loaded thioether (1.2umole gear) was oxidised with m-chloroperbenzoic acid (5eq, 65% reagent, 1.6mg) in dichloromethane (200uL) for 5hrs, followed by standard washing and then cleaved to provide EXAMPLE 76.
EXAMPLE 77. (3aS, 6aK) 2^-Cyclohexylmethyl-4-morpholin-4-yl-l-(3-oxo-hexa hydrofuro[3,2-6]pyrrol-4-yl)butane-l,4-dione
HPLC-MS 393.1 [M + H]+, 807.3 [2M + Naf.
(a) Preparation of 2/?-cyclohexylmethyl-4-morpholin-4-yl-4-oxo-butyric acid methyl ester.
l-(3-dimethylarninopropyl)-3-ethylcarbodiirnide.HCl (1.12g, 5.69mmol) then 1-hydroxybenzotriazole monohydrate (0.87g, 5.69mmol) were added to a stirred solution of 2^?-(cyclohexylmethyl)succinic acid 1 -methyl ester (compound (35),1.0g, 4.38mmol) in dimethylformamide (10ml) at 0°C under argon. The mixture was stirred for 25minutes then morpholine (0.7 ml, 8.76mmol) was added drop-wise over 1 minute and stirring continued at ambient temperature for 16hours. The product was extracted into ethyl acetate (200ml) then washed with l.OM hydrochloric acid (3 x 100ml), saturated aqueous sodium hydrogen carbonate solution (3 x 100ml), water (100ml), then saturated aqueous sodium chloride solution (100ml), dried (MgSCU), and the solvent removed in vacua to give 2/?-cyclohexylmernyl-4-morpholin-4-yl-4-oxo-butyric acid methyl ester as an off-white solid, yield 1.22g, (94%). HPLC-MS (single peak with Rt = 7.91mins, 298.1 [M + H]+, 617.3 [2M + Naf).
(b)Preparation of 2/2-cyclohexylmethyl-4-morpholin-4-yl-4-oxo-butyric acid (Compound (36), Scheme 7).
A solution of lithium hydroxide monohydrate (O.Slg, 12.18mmol) in water (27ml) was added a stirred solution of 2JR-cyclohexyhnethyl-4-morpholin-4-yl-4-oxo-butyric acid methyl ester (1.21g,, 4.06mmol) in tetrahydrofuran (55ml) and methanol (27ml) at 0°C. The mixture was stirred at ambient temperature for 1 hours then diluted with water (100ml). The aqueous layer was extracted with diethyl ether (2 x 50ml) which was discarded, then acidified to pH = 1-2 with 1M hydrochloric acid. The product was extracted into dichloromethane (3 x 50ml), then the combined ethereal layers washed with water (2 x 50ml), saturated aqueous sodium chloride solution (2 x 50ml), dried (MgSO4) and the solvent removed in vacua to leave a residue. The residue was purified by chromatography over silica gel eluting with a gradient of
methanol : dichloromethane 1:100 -> 3:100. Appropriate fractions were combined and the solvents removed in vacuo was to give 2#-cyclohexylmemyl-4-morpholin-4-yl-4-oxo-butyric acid (36) as a white solid, yield 0.82 g, (71%). HPLC-MS (single peak with Rt = 6.769mins, 284.2 [M + Hf, 589.2 [2M + Naf).
5H (400MHz, CDC13 at 298K),0.77-0.90 (2H, CH2(cyclo} ie), m), 1.05-1.40 (4H, CH2(cyclohexane),m), 1.50-1.90 (7H, CH(cyclohexaik CH2(cyclohexane), m), 2.30-2.44 (2HJ3, m), 2.64-2.77 (IHa, m), 2.96-3.10 (1H, OH, brs), 3.40-3.78 (8H, CH2OCH2 and CH2NCH2 ,m).
Compound (36) was coupled under standard conditions to loaded building block-linker construct (22), then cleaved to provide EXAMPLE 77.
EXAMPLE 78. (3aS, 6aR) 4-(2-Biphenyl-3-yl-4-
methylpentanoyl)tetrahydrofuro[3,2-6]pyrrol-3 -one
HPLC Rt = 19.09-20.76mins (>90%), HPLC-MS 378.4 [M + H]+
(a)Preparation of Biphenyl-3-yl-acetic acid methyl ester (Compound (38), Scheme 8)
Concentrated sulphuric acid (588uL) was added to a solution of 3-bromophenyl acetic acid (lOg, 46.5mmol) in methanol (lOOmL). The mixture was refiuxed for 1.5h and then cooled to ambient temperature and evaporated under reduced pressure to afford a residue. The residue was redissolved in diethyl ether (500mL), washed with water (2 x lOOmL), brine (lOOmL), dried (MgSO4) and then evaporated under reduced pressure to afford 3-bromophenyl acetic acid methyl ester (10.65g). The 3-bromophenyl acetic acid methyl ester was dissolved in toluene (117mL) then phenyl
boronic acid (6.8g, 55.69mmol) added, followed by a aqueous solution of sodium carbonate (93mL, 2M) and tetrakis(triphenylphosphine)palladium (1.6g, 1.41mmol). The mixture was stirred overnight then cooled to ambient temperature and an aqueous solution of saturated ammonium chloride (lOOmL) added. The mixture was extracted with ethyl acetate (2 x 200mL), died (Na2SO4) and evaporated under reduced pressure to afford a residue. Flash chromatography of the residue over silica (200g) using ethyl acetate : heptane (3 : 48) as the eluent gave biphenyl-3-yl acetic acid methyl ester, yield 10.5g, (99 %), TLC (single UV spot, R{ = 0.24, 10% ethyl acetate in heptane), analytical HPLC Rt - 19.55 min, HPLC-MS (single main UV peak with Rt = 9.35 min, 227.1 [M + H]*).
5H (400MHz, CDC13 at 298K) 3.76 (2H, s, CH2CO2CH3), 3.77 (3H, s, OCH3\ 7.34-7.66 (9H, m, biphenyl-3-yl).
(b)Preparation of Biphenyl-3-yl-acetic acid
Water (39mL), followed by lithium hydroxide monohydrate (4.2g, lOl.Smmol) were added to a solution of biphenyl-3-yl acetic acid methyl ester (11.43g, 50.57mmol) in methanol (265mL). The mixture was stirred at ambient temperature for 2h then the organics were removed under reduced pressure. The mixture was acidified with dilute hydrochloric acid (1M, 80mL), extracted with chloroform (2 x lOOmL), dried (MgS04) and evaporated under reduced pressure to afford biphenyl-3-yl acetic acid as a white solid, yield 10.6g, (99%), analytical HPLC /?t = 16.565 min, HPLC-MS (single main UV peak with RI = 7.91mm, 213. 1 [M + Hf).
8H (400MHz, CDC13 at 298K) 3.77 (2H, s, C^CC^CHj), 7.28-7.52 (9H, m, biphenyl-3-yl).
(c)Preparation of 2/?S-Biphenyl-3-yl-4-rnethylpent-4-enoic acid
A solution of biphenyl-3-yl acetic acid (7.0g, 33mmol) in anhydrous tetrahydrofuran (84mL) was added dropwise to a solution of lithium diisopropyl amide (36.4mL, 2M
solution in hexanes) in anhydrous tetrahydrofuran (84mL) at -78 °C. The mixture was allowed to warm to 0 °C and stirred for 40 min. The mixture was then cooled to -78 °C and 3-bromo-2-methylpropene (4.97mL) rapidly added. The mixture was stirred for 1 h at -78 °C then water (28mL) added and the organics removed under reduced pressure. The mixture was then acidified with hydrochloric acid (6M, 14ml), extracted with ethyl acetate (3 x 100 ml), dried (MgS04) and evaporated under reduced pressure to afford a residue. Flash chromatography of the residue over silica (400g) using methanol : dichloromethane (3 : 97) as the eluent afforded impure 2-biphenyl-3-yl-4-methylpent-4-enoic acid (8.3 g). Flash chromatography over silica (400g) using methanol: dichloromethane (1.5 : 98.5) afforded pure 2-biphenyl-3-yl-4-methylpent-4-enoic acid, yield 5.27g, (60 %), TLC (single UV spot, Rf = 0.28, 5 % methanol hi dichloromethane), analytical HPLC J?t = 19.99 min, HPLC-MS (single main UV peak with ft = 9.57 min, 267.1 [M + H]4).
SH (400MHz, CDC13 at 298K), 1.765 (3H, s, C#3)> 2.53 (1H, dd, J= 6.6 and 14.7 Hz, 3-Hi), 2.91 (1H, dd, J= 8.9 and 14.7 Hz, 3-Hi), 3.92 (1H, dd, /= 6.6 and 8.9 Hz, 2-H), 4.79 (2H, d, J= 10.7 Hz, 5-H2), 7.30-7.62 (9H, m, biphenyl-3-yl).
(d)Preparation of 2&S-Biphenyl-3-yl-4-methylpentanoic acid (Compound (39), Scheme 8)
Palladium on carbon (10%, 300mg) was added portionwise to a solution of 2RS-biphenyl-3-yl-4-methylpent-4-enoic acid (Ig, 3.76mmol) in ethanol (40mL) at 0 °C. A hydrogen atmosphere was then introduced and the mixture allowed to warm to ambient temperature. The mixture was stirred for 18h, then the hydrogen atmosphere removed and the mixture filtered over Celite and the catalyst washed with ethanol (40mL). The combined organic filtrate was concentrated under reduced pressure to afford a residue, which was flash chromatographed over silica (150g) using methanol : dichloromethane (1 : 99) as the eluent to afford 2^1S-biphenyl-3-yl-4-methylpentanoic acid, yield 980mg, (98%), TLC (single UV spot, R{ = 0.45, 5 % methanol in dichloromethane), analytical HPLC Rt = 20.92 min, HPLC-MS (single main UV peak with Rt = 10.15 min, 269.1 [M + H]+, 291.1 [M+Na]4).
5H (400MHz, CDC13 at 298K), 0.93 (6H, d, J= 6.6 Hz, 2 x CH3), 1.52-1.57 (IH, m, 4-HO, 1.71-1.76 (IH, m, 3-Hi), 1.97-2.05 (IH, m, 3-Hi), 3.66 (IH, t, J= 7.8 Hz, 2-Hi), 7.32-7.60 (9H, m, biphenyl-3-yl).
Compound (39) was coupled under standard conditions to loaded building block-linker construct (22), then cleaved to provide EXAMPLE 78.
EXAMPLE 79. (3aS, 6aK) 4-Methyl-JV-[3-methyl-15-(3-oxo-hexahydrofuro[3,2-&] pyrrole-4-carbonyl) butyl]benzamide
HPLC Rt = 13.74-14.85mins (> 90%), HPLC-MS 359.2 [M + Hf.
HPLC Rt = 12.95-13.87mins (> 90%), HPLC-MS 375.2 [M + Hf.
EXAMPLE 80. (3aS, 6aR) 4-Methoxy-^-[3-methyl-15-(3-oxo-hexahydrofuro[3,2-6]pyrrole-4-carbonyl) butyl]benzamide
(Figure Remove)-174- ^-Butyl-N-[lS-(4-bromobenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyrrol-4-yl)ethyl]benzamide
(Figure Remove)HPLC Rt = 18.40-19. lOmins (> 90%), HPLC-MS 513.2 / 515.2 [M + Hf.
EXAMPLE 82. (3aS, 6aR) 4-fert-Butyl-]V-[15-(4-methoxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fc]pyrrol-4-yl)ethyl]benzamide
HPLC Rt = 17.50-18.31mins (> 90%), HPLC-MS 465.2 [M + H]+.
EXAMPLE 83 (An example of general formula (II)). (3aS, laK) Morpholine-4-carboxylic acid [l/?-ben2ylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3J2-^]pyridin-4-yl)ethyl] amide
The required bicycle building block (3a5, 7aR) 3-oxo-hexahydrofuro[3,2-6]pyridine-4-carboxylic acid 9H-fluoren-9-ylmethyl ester was prepared in 8 steps as follows.
(1) Preparation of Cz5-7-ac-(3-hydroxy)pyrrolidine-l,2-dicarboxylic acid 1-(9H-fluoren-9-ylmethyl) ester.
O'5r-rac-(3-hydroxy)piperidine-2-carboxylic acid (obtained by hydrogenation of commercially available 3-hydroxypicolinic acid, see Drummond, J. et al, J. Med. Chem., 32, 2116-2128, 1989) (15.0g, 103.4mmole) was added to a vigorously stirred, ice-cooled solution of sodium carbonate (22.90gs 217.1mmole) in water (200mL). 1,4-Dioxan (lOOmL) was added providing a clear pale yellow solution. 9-Fluorenylmethyl chloroformate (27.52g, 108.4mmole) in 1,4-dioxan (lOOrnL) was added over Ihr, then the ice-cooling removed and the mixture stirred at RT for an additional Ihr. Additional water (150mL) was added, the reaction mixture washed with chloroform (2 x 250mL) and the combined organic layers discarded. The aqueous phase was acidified with IN HC1 to ~ pH 2, providing a thick opaque mixture. The acidified aqueous mixture was extracted with chloroform (2 x SOOmL) and the now clear aqueous phase discarded. The opaque combined chloroform layers were dried (NaiSO-O, filtered and reduced in vacuo to provide a white foam, yield 30.2g (82.2mmole, 79.5%). TLC (Minor UV spot, Rf = 0.58, major UV spot Rf = 0.25, methanol: chloroform 1:4), analytical HPLC major peak Rt = 16.990mins, HPLC-MS (single major UV peak with Rt = 7.976mins, 368.0 [M + H]+, 390.0 [M + Naf. Analysis by LH and 13C NMR
showed the presence of cis and trans geometrical isomers around the 3° amide bond.
5C (DMSO-d6 at 298K); 23.29/23.57 (d, C5), 28.89 (d, Cy), 40.19 (d, Ce), 47.59 (u, Fmoc C-9), 59.11/59.43 (u, Ca), 67.34 (u, Cp), 67.75/67.87 (d, Fmoc CH2), 121.13 (u, Fmoc C-4 and C-5), 126.90/127.02 (u, Fmoc C-l and C-8), 128.11 (u, Fmoc C-2 and C-7), 128.68 (u, Fmoc C-3 and C-6), 141.73 (q, Fmoc C-4' and C-5'), 144.58/144.76 (q, Fmoc C-l' and C-8'), 155.71/156.23 (q, OCON), 172.37/172.63 (COOH).
(2) Preparation of Cw-rac-(3-hydroxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester l-(9H-fluoren-9-ylmethyl) ester.
Cw-7-ac-(3-hydroxy)pyrrolidine-1,2-dicarboxylic acid 1 -(9H-fluoren-9-yl methyl) ester (28.2g, 76.7mmole) was dissolved in toluene (200mL) in a Dean-Stark apparatus. Allyl alcohol (50mL) was added followed by p-toluenesulphonic acid.hydrate (15.Ig, 78.2mmole). The mixture was refluxed for Ihr, cooled and CHCls (SOOmL) added. The organic layer was washed with NaHCO3 (2 x 250mL), 0.1N HC1 (250mL) and brine (250mL), then dried (Na2SO4). Filtration and reduction in vacuo gave a pale yellow mobile oil (35g). The crude oil was purified over silica gel (240g) eluting with a gradient of heptane : ethyl acetate 3:1 -^ 1:1. Desired fractions were combined and reduced in vacuo to a colourless gum yield 20.4g (SO.lmmole, 65.3%). TLC (single UV spot, Rf = 0.20, heptane : ethyl acetate 2:1), analytical HPLC Rt = 20.003mins, HPLC-MS (single main UV peak with Rt = 9.473mins, 408.1 [M + Hf, 430.1 [M + Naf). Analysis by !H and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond.
5H (CDC13 at 298K); 1.55 (2Hyor 2H5, d, J9.3), 1.81 (lHy or 1H5, d, J3.2), 2.06 (lHYor 1H5, d, J3.2), 2.78/2.92 (1HE, dbt), 3.55 (1HP, dd, J12.6, 9.2), 3.78 (OH, b), 3.95/4.11 (1H£, d, J13), 4.31 (Fmoc H-9, m), 4.49 (Fmoc CHj, m), 4.71 (2 x COOCH2CH=CH2, m), 4.99/5.17 (Ha, d, J4.8), 5.31 (2 x COOCH2CH=CH2j m), 5.87 (1 x COOCH2CH=CH2, m), 7.37 (2H aromatic,
Fmoc H-2 and H-7), 7.47 (2H aromatic, Fmoc H-3 and H-6), 7.62 (2H aromatic, Fmoc H-l and H-8), 7.83 (2H aromatic, Fmoc H-4 and H-5). 6C (CDC13 at 298K); 23.87/24.11 (d, C5), 30.59 (d, Cy), 41.26/41.56 (d, Ce), 47.62 (u, Fmoc C-9), 58.29/59.54 (u, Ctt), 66.44/66.54 (d, COOCH2CH=CH2), 68.28 (d, Fmoc CH2), 69.14/69.42 (u, Cp), 119.67/119.90 (d, COOCH2CH=CH2), 120.43 (u, Fmoc C-4 and C-5), 125.22/125.35 (u, Fmoc C-l and C-8), 127.48 (u, Fmoc C-2 and C-7), 128.15 (u, Fmoc C-3 and C-6), 131.43/131.57 (u, COOCH2CH=CH2), 141.73 (q, Fmoc C-4' and C-5'), 144.19/144.38 (q, Fmoc C-l' and C-8'), 155.67/156.27 (q, OCON), 171.56 (COOCH2CH=CH2).
(3) Enzymic Resolution of Cis-rac-(3-hydroxy)pyrroUdine-l,2-dicarboxylic acid 2-allyl ester l-(9H-fluoren-9-ylmethyl) ester to (2R, 35) (3-hydroxy) pyrrolidine-l,2-dicafboxylic acid 2-allyl ester l-(9H-fluoren-9-ylmethyl) ester and (25, 2>R) (3-acetoxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester l-(9H-fluoren-9-ylmethyl) ester.
Cw-rac-(3-hydroxy)pyrrolidine-l,2-dicafboxylic acid 2-allyl ester 1-(9H-fluoren-9-ylmethyl) ester (19.6g, 48.1mmole), Lipase PS (25g, ex Amano Enzyme Inc.), vinyl acetate (300mL) and isopropyl ether (200mL) were stirred at 38°C. After 5 days, HPLC indicated equilibration of 2 peaks (52% : 48%, indicating 95% conversion) and the reaction mixture was filtered through celite (3cm x 10cm bed). The celite bed was washed with chloroform (3 x 250mL) and the combined organic filtrate reduced in vacuo to a viscous tan oil (23.Og). The crude oil was purified over silica gel (300g) eluting with a gradient of heptane : ethyl acetate 3:1 -^ 1:2. Two major fractions were identified and reduced in vacuo.
Product 1. (25, 2>K) (3-acetoxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester l-(9H-fluoren-9-yknethyl) ester.
A viscous very pale yellow oil, yield 9.5g (21.1mmole, 43.9%). TLC (single UV spot, Rf = 0.40, heptane : ethyl acetate 2:1), analytical HPLC Rt = 22.633mins, HPLC-MS (single main UV peak with Rt = 10.714mins, 450.1
[M + Hf, 472.1 [M + Na]4). Analysis by JH and ]3C NMR showed the
presence of cis and trans geometrical isomers around the 3° amide bond.
5H (CDC13 at 298K); 1.25-1.32/1.52-1.70/1.72-2.00 (2HY + 2H5, bm), 2.05-
2.15 (OCOCHj, bs), 3.35-3.58 (1HE, m), 3.98-4.10/4.18-4.22 (1HE, dd),
4.24-4.31 (Fmoc H-9, m), 4.32-4.52 (Fmoc CKg, m), 4.60-4.82 (2 x
COOCH2CH=CH2, m), 4.92-4.99 (Hp, m), 5.22-5.32 (Ha, m), 5.28-
5.32/5.34-5.41 (2 x COOCH2CH=CH2, dd, .717.2), 5.89-6.00 (1 x
COOCH2CH=CH2, m), 7.30-7.37 (2H aromatic, Fmoc H-2 and H-7), 7.38-
7.46 (2H aromatic, Fmoc H-3 and H-6), 7.59-7.76 (2H aromatic, Fmoc H-1
and H-8), 7.78-7.80 (2H aromatic, Fmoc H-4 and H-5, d, J7.5).
5C (CDC13 at 298K); 21.22 (u, OCOCH3), 23.01 (d, C5), 25.54 (d, Cy),
40.51/40.88 (d, CE), 47.44 (u, Fmoc C-9), 55.55 (u, Ca), 66.08 (d,
COOCH2CH=CH2), 68.30/68.65 (d, Fmoc CH2), 69.21/69.59 (u, Cp),
118.94/119.22 (d, COOCH2CH=CH2), 120.31 (u, Fmoc C-4 and C-5),
125.34/125.49 (u, Fmoc C-1 and C-8), 127.43 (u, Fmoc C-2 and C-7), 128.04
(u, Fmoc C-3 and C-6), 131.93 (u, COOCH2CH=CH2), 141.61 (q, Fmoc C-4'
and C-5'), 144.15 (q, Fmoc C-1' and C-8'), 155.50/156.36 (q, OCON),
169.42/169.91/170.37 (COOCH2CH=CH2 + OCOCH3).
Product 2. (2R, 35) (3-hydroxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester l-(9H-fluoren-9-ylmethyl) ester.
A white crystalline solid, yield 9.05g (22.2mmole, 46.1%). TLC (single UV spot, Rf = 0.20, heptane : ethyl acetate 2:1), analytical HPLC Rt = 19.970mins, HPLC-MS (single main UV peak with Rt = 9.450mins, 408.1 [M + H]+, 430.1 [M + Na]4). Analysis by *H and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond. 5H (CDC13 at 298K); 1.47-1.61 (lHy+ 1H5, m), 1.71-1.81 (1H5, b), 2.02-2.11 (1HY> b), 2.71-2.81/2.84-2.94 (1HE, dt), 3.54-3.59 (Hp, m), 3.70-3.82 (OH, b), 3.93-3.96/4.10-4.16/4.11 (1H6, d, JIO), 4.24-4.31 (Fmoc H-9, m), 4.40-4.56 (Fmoc CH^, m), 4.61-4.78 (2 x COOCH2CH=CH2} m), 4.98/5.18 (Ha, d, J5.0), 5.25-5.37 (2 x COOCH2CH=CH2, m), 5.86-5.96 (1 x
COOCH2CH=CH2, m), 7.30-7.34 (2H aromatic, Fmoc H-2 and H-7), 7.41-7.48 (2H aromatic, Fmoc H-3 and H-6), 7.53-7.62 (2H aromatic, Fmoc H-l and H-8), 7.79-7.81 (2H aromatic, Fmoc H-4 and H-5). 8C (CDC13 at 298K); 23.87/24.12 (d, C6), 30.60 (d, C,\ 41.27/41.57 (d, Ce), 47.62 (u, Fmoc C-9), 58.29/59.54 (u, Ca), 66.44/66.54 (d, COOCH2CH=CH2), 68.28 (d, Fmoc CH2), 69.15/69.42 (u, Cp), 119.67/119.91 (d, COOCH2CH=CH2), 120.42 (u, Fmoc C-4 and C-5), 125.22/125.35 (u, Fmoc C-1 and C-8), 127.48 (u, Fmoc C-2 and C-7), 128.15 (u, Fmoc C-3 and C-6), 131.42/131.57 (u, COOCH2CH=CH2), 141.73 (q, Fmoc C-4' and C-5'), 144.12/144.37 (q, Fmoc C-1' and C-8'), 155.67/156.26 (q, OCON), 171.57 (COOCH2CH=CH2).
(4) Preparation of (25, 3£) (3-hydoxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester l-(9H-fluoren-9-ylmethyl) ester.
(25, 3K) (3-acetoxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester 1-(9H-fluoren-9-ylmethyl) ester (4.5g, lOmmole) ) was dissolved in allyl alcohol (50mL), cH2SO4 (200uL) added and heated to reflux for 24hrs. The mixture was cooled, EtOAc (250mL) added and washed with NaHCOj (250mL), brine (250mL) and dried (Na2SC«4). The solvents were removed in vacuo to give a tan oil (4.2g). The crude oil was purified over silica gel (170g) eluting with a gradient of heptane : ethyl acetate 3:1 -> 1:1. Desired tractions were combined and reduced in vacuo to a white crystalline solid, yield 3.0g (7.37mmole, 73.7%). TLC (single UV spot, Rf = 0.20, heptane : ethyl acetate 2:1), analytical HPLC Rt = 19.474mins, HPLC-MS (single main UV peak with Rt = 9.507mins, 408.2 [M + Hf, 430.2 [M + Naf). Analysis by 1H and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond.
5H (CDC13 at 298K); 1.46-1.59 (1HY+ 1H8, m), 1.74-1.81 (1H8, b), 2.01-2.10 (lHy, b), 2.70-2.82/2.88-2.99 (lHe, dt), 3.49-3.62 (Hp, m), 3.70-3.80 (OH, b), 3.90-3.94/4.09-4.14 (1H6, dd), 4.26-4.32 (Fmoc H-9, m), 4.41-4.58 (Fmoc CH^, m), 4.60-4.80 (2 x COOCH2CH=CH2, m), 4.96-4.98/5.17-5.18 (Ha, dd, J4.8), 5.25-5.42 (2 x COOCH2CH=CH2, m), 5.82-5.97 (1 x
COOCH2CH=CH2, m), 7.30-7.38 (2H aromatic, Fmoc H-2 and H-7), 7.42-7.48 (2H aromatic, Fmoc H-3 and H-6), 7.55-7.63 (2H aromatic, Fmoc H-l and H-8), 7.79-7.84 (2H aromatic, Fmoc H-4 and H-5). 5C (CDC13 at 298K); 23.86/24.12 (d, C8), 30.62 (d, Cy), 41.28/41.57 (d, CE), 47.62 (u, Fmoc C-9), 58.29/59.54 (u, Ca), 66.43/66.54 (d, COOCH2CH=CH2), 68.27 (d, Fmoc CH2), 69.15/69.43 (u, Cp), 119.69/119.91 (d, COOCH2CH=CH2), 120.42 (u, Fmoc C-4 and C-5), 125.22/125.35 (u, Fmoc C-l and C-8), 127.47 (u, Fmoc C-2 and C-7), 128.14 (u, Fmoc C-3 and C-6), 131.43/131.57 (u, COOCH2CH=CH2), 141.73 (q, Fmoc C-4' and C-5'), 144.12/144.37 (q, Fmoc C-l' and C-8'), 155.67/156.26 (q, OCON), 171.57 (COOCH2CH=CH2).
(5) Preparation of (2S, 3K) (3-tert-butoxy)pyrrolidine-l ,2-dicarboxylic acid 2-allyl ester l-(9H-fluoren-9-ylmethyl) ester.
(25, 3K) (3-hydoxy)pyrrolidine-l ,2-dicarboxylic acid 2-allyl ester 1-(9H-fluoren-9-ylmethyl) ester (1.75g, 4.30mmole) was dissolved in dry dichloromethane (20mL) in a SOmL glass pressure tube and cooled to -78°C. Isobutylene gas (~10mL) was condensed into the solution and cH2SC«4 (lOOuL) added. A stirrer bar was added, the tube was sealed, the cooling removed and stirred at RT for 72hr. The sealed tube was cooled to -78°C, N-methylmorpholine (200uL, leq w.r.t. cH2SO4) and allowed to warm to RT, unsealed, with stirring over 2hr. Dichloromethane (75mL) was added and the organics washed with NaHCO3 (75mL), then brine (75mL) and dried (Na2SO4). The solvents were removed in vacuo to give a pale tan oil (1.91g). The crude oil was purified over silica gel (lOOg) eluting with a gradient of heptane : ethyl acetate 5:1 -^ 3:1. Desired fractions were combined and reduced in vacuo to a thick clear oil yield 1.59g (3.43mmole, 79.8%). TLC (single UV spot, Rf= 0.50, heptane : ethyl acetate 2:1), analytical HPLC Rt = 24.123mins, HPLC-MS (single main UV peak with Rt = 11.91mins, 408.2 [M + H-Bu'f, 486.3 [M + Naf, 949.5 [2M + Na]4). Analysis by !H and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond.
sH (CDC13 at 298K); 1.23 (9 x C(CH3)3, s), 1.46-1.98 (2HY+ 2H5, m), 3.37-3.45/3.46-3.57 (1HE, dt), 3.61-3.72 (Hp, b), 3.90-3.99/4.02-4.10 (lHe, dd), 4.20-4.72 (Fmoc H-9 + Fmoc CH^ + 2 x COOCH2CH=CH2, bm), 4.89/5.01 (Ha, dd, J6.3), 5.24 (1 x COOCH2CH=CH2, d, J10.5), 5.38 (1 x COOCH2CH=CH2, d), 5.88-5.99 (1 x COOCH2CH=CH2, m), 7.29-7.36 (2H aromatic, Fmoc H-2 and H-7), 7.38-7.47 (2H aromatic, Fmoc H-3 and H-6), 7.56-7.69 (2H aromatic, Fmoc H-1 and H-8), 7.76-7.81 (2H aromatic, Fmoc H-4 and H-5).
5C (CDC13 at 298K); 23.69/23.99 (d, C8), 28.38/28.65 (u, C(CH3)3), 28.75 (d, q), 40.40/40.72 (d, Ce), 47.61 (u, Fmoc C-9), 58.36/58.47 (u, Ca), 65.67 (d, COOCH2CH=CH2), 67.82 (u, Cp), 67.99/68.13 (d, Fmoc CH2), 74.97 (q, C(CH3)3), 118.44/118.57 (d, COOCH2CH=CH2), 120.40 (u, Fmoc C-4 and C-5), 125.39/125.48 (u, Fmoc C-l and C-8), 127.46 (u, Fmoc C-2 and C-7), 128.10 (u, Fmoc C-3 and C-6), 132.49 (u, COOCH2CH=CH2), 141.71 (q, Fmoc C-4' and C-5'), 144.14/144.30 (q, Fmoc C-l' and C-8'), 155.44/157.00 (q, OCON), 170.43/171.05 (COOCH2CH=CH2).
(6) Preparation of (2S, 3K) (3-tert-butoxy)pyrrolidine-l,2-dicarboxylic acid 1-(9H-fluoren-9-ylmethyl) ester.
(25,3^) (3-fe7-/-butoxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester 1-(9H-fluoren-9-ylmethyl) ester (1.52g, 3.29mmole) was dissolved in dry dichloromethane (25mL) with stirring. Tetrakistriphenylphospuine palladium (0) (76mg, 0.066mmole, 0.02eq) was added, followed by phenyltrihydrosilane (0.71 g, 0.622mL, 6.58mmole, 2eq). After Ihr, dichloromethane (150mL) was added and the organics washed with 0.01N HC1 (ISOmL), brine (150mL) and dried (Na2SO4). The solvents were removed in vacuo to give a dark grey solid (2.05g). The crude solid was purified over silica gel (75g) eluting with a gradient of heptane : ethyl acetate 2:1 -> 1:2. Desired fractions were combined and reduced in vacuo to a white crystalline yield 0.97g (2.29mmole, 69.6%). TLC (single UV spot, Rf = 0.25, heptane : ethyl acetate 1:1), analytical HPLC Rt = 21.310mins, HPLC-MS (single main UV peak with Rt = 10.254mins, 368.2 [M + H-Bulf, 446.2 [M
+ Na]+, 869.3 [2M + Na]4). Analysis by 1E and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond. 5H (CDC13 at 298K); 1.32/1.40 (9 x C(CH3)3, ds), 1.42-1.58 (1HY+ 1H5, m), 1.72-1.80 (1H5, bm)3 1.82-1.96 (1HY, bm), 2.76-2.82/2.90-2.97 (1H£5 dt), 3.70-3.84 (Hp, b), 3.90-4.07 (lHe, dd), 4.22-4.32 (Fmoc H-9), 4.40-4.58 (Fmoc CH2, bm), 4.59-4.61/5.02-5.04 (Ha, dd, J5.7), 7.29-7.36 (2H aromatic, Fmoc H-2 and H-7), 7.39-7.43 (2H aromatic, Fmoc H-3 and H-6), 7.53-7.67 (2H aromatic, Fmoc H-l and H-8), 7.76-7.81 (2H aromatic, Fmoc H-4 and H-5), 10.90-11.10 (COOH,bs).
6C (CDC13 at 298K); 23.71/24.23 (d, C5), 28.21/28.28 (u, C(CH3)3), 30.58/30.72 (d, Cy), 40.49/40.79 (d, Ce), 47.58/47.63 (u, Fmoc C-9), 58.20/58.43 (u, Ca), 67.78/68.32 (u, Cp), 68.69/68.88 (d, Fmoc CH2), 78.48/78.67 (q, C(CH3)3), 120.31 (u, Fmoc C-4 and C-5), 125.16/125.27/125.50/125.53 (u, Fmoc C-l and C-8), 127.42/127.46/127.59 (u, Fmoc C-2 and C-7), 128.03/128.10 (u, Fmoc C-3 and C-6)5 141.55/141.69/141.79 (q, Fmoc C-4' and C-5'), 144.06/144.12/144.38/144.48 (q, Fmoc C-l' and C-8')5155.73/156.49 (q, OCON), 169.26/169.52 (COOH).
(7) Preparation of (2S, 3R) (3-ter^butoxy)-2-(2-diazoacetyl)pyn:oHdine-l32-dicarboxylic acid l-(9H-fluoren-9-ylmethyl) ester.
(25, 37?) (3-ter/-butoxy)pyrrolidme-l,2-dicarboxylic acid 1-(9H-fluoren-9-yhnethyl) ester (830mg, 1.96mmole) was dissolved with stirring in dry dichloromethane (20mL). The reaction was flushed with nitrogen and cooled to -15°C. Isobutylchloroformate (296mg, 2.16mmole in dry dichloromethane (2.5mL) and N-methyhnorpholine (397mg, 3.92mmole in dry dichloromethane (2.5mL) were added simultaneously in O.SmL aliquots over ISmrns. Etheral diazomethane (generated from diazald (2.5g, ~8mmole in diethyl ether (40mL)) onto sodium hydroxide (2.75g) in water (4.3mL) / ethanol (8.6mL) at 60°C). was added to the activated aminoacid solution and stirred at RT for 24hr. Acetic acid (~2mL) was added to quench the reaction, then fe/t-butyhnethylether (lOOmL) was added, the organics washed with water (3 x 150mL), then dried (Na2SO4). The solvents were removed in
vacuo to give a thick yellow oil (1.02g). The crude oil was used for the next stage without purification. TLC (single main UV spot, Rf = 0.40, heptane : ethyl acetate 2:1), analytical HPLC Rt = 18.887mins (50.8%) plus numerous minor peaks, HPLC-MS (2 main UV peaks with Rt = 9.095mins, 364.2 [M + Hf, 749.2 [2M + Naf and Rt = 10.856mins, 420.2 [M + H-N2]+, 470.2 [M + Naf, 917.3 [2M + Naf).
Note that upon HPLC-MS analysis, m/z 364.2 corresponds to the desired bicycle product (3aS, 7a/?) 3-oxo-hexahydrofuro[3,2-Z)]pyridme-4-carboxylic acid 9#-fluoren-9-ylmethyl ester.
(8) Cyclisation to (3aS, laK) 3-oxo-hexahydrofuro[3,2-6]pyridine-4-carboxy lie acid 9/f-fluoren-9-ylmethyl ester.
A solution of lithium chloride (844mg, 20mmole) in water (5mL) and acetic acid (20mL) was added to (25, 3R) (3-/ert-butoxy)-2-(2-diazoacetyfypyrrolidine-l^-dicarboxylic acid l-(9H-fluoren-9-ylmethyl) ester (lOOOmg, ~2mmole). Gas was evolved and the yellow oily solid dissolved over Ihr to give a virtually colourless solution. After 90mins, chloroform (150mL) was added and the organics washed with NaHCO3 (2 x ISQmL), brine (150mL) and dried (NaaSO/j). The solvents were removed in vacuo to give a pale yellow gum (920mg). The crude gum was purified over silica gel (135g) eluting with a gradient of heptane : ethyl acetate 3:1 -> 1:1. Desired fractions were combined and reduced in vacuo to a white crystalline solid, yield 370mg (1.02mmole, 51.9% from starting acid). TLC (single UV spot, Rf = 0.25, heptane : ethyl acetate 2:1), analytical HPLC Rt = 18.734mins, HPLC-MS (single UV peak with Rt = 9.051mins, 364.2 [M + Hf, 386.2 [M + Na]4). Analysis by !H and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond.
sH (CDC13 at 298K); 1.29-1.49 (1HY+ 1H5, m), 1.61-1.80 (1H5, bm), 2.06-2.19 (1HY, bm), 2.50-2.62/2.63-2.80 (1H6, m), 3.96 (1HE, b), 3.97 (COCHiA, d, J16.3), 4.15 (Fmoc H-9, bt), 4.25-4.34 (Hp, b), 4.36-4.60 (Fmoc CHi + COCH2B, bm), 4.75-4.82/5.11-5.19 (Ha, bd), 7.30-7.36 (2H aromatic, Fmoc H-2 and H-7), 7.41-7.49 (2H aromatic, Fmoc H-3 and H-6), 7.52-7.68
(2H aromatic, Fmoc H-l and H-8), 7.75-7.85 (2H aromatic, Fmoc H-4 and H-5).
SC (CDC13 at 298K); 21.75/22.04 (d, C5), 26.60 (d, Cy), 41.40/41.66 (d, CE), 47.59 (u, Fmoc C-9), 60.42 (u, Ca), 67.54 (d, Fmoc CH2), 68.21/68.39 (d, COCH2O), 72.61 (u, Cp), 120.39 (u, Fmoc C-4 and C-5), 125.43 (u, Fmoc C-1 and C-8), 127.51 (u, Fmoc C-2 and C-7), 128.14 (u, Fmoc C-3 and C-6), 141.73 (q, Fmoc C-41 and C-5'), 144.30 (q, Fmoc C-l' and C-8'), 155.79/156.35 (q, OCON), 211.10/211.4 (q, COCH2O).
Following the general details from Scheme 2, the reqmred bicycle building block (3aS, laK) 3-oxo-hexahydrofuro[3,2-fc]pvridine-4-carboxylic acid 9ff-fluoren-9-ylmethyl ester was converted to building block-linker construct as follows:
(3aS, 7a/?) 3-oxo-hexahydrofuro[3,2-6]pyridine-4-carboxylic acid 9//-fluoren-9-ylmethyl ester (250mg, 0.689mmole) was dissolved in a mixture of ethanol (12.0mL) and water (1.75mL) containing sodium acetate.trihydrate (141mg, 1.03mmole, 1.5eq). 4-[[(hydrazinocarbonyl)amino]methyl]cyclo hexanecarboxylic acid, trifluoroacetate (227mg, 0.689mmole, l.Oeq, Murphy, A. M. et al, J. Am. Chem. Soc., 114, 3156-3157, 1992) was added and the mixture refluxed for 4brs. Chloroform (150mL) was added and the organics washed with HC1 (150mL, ~ pH3), dried (NaaSC^) and reduced in vacuo to provide crude building block-linker construct a clear viscous oil. Yield 350mg, analytical HPLC 2 peaks Rt = 17.101 (74.7%) and 18.547mins (25.3%) (cis / trans geometrical isomers), HPLC-MS (2 x UV peak with Rt = 8.037 and 8.972mins, 561.2 [M + H]*). Crude construct was used directly for construct loading.
Following the general details from Scheme 2, the required building block-linker construct was attached to the solid phase providing loaded building block-linker construct as follows:
Building block-linker construct (0.595mmoles), 2-(lH-benzotriazole-l-yl)-l, 1,3,3-tetramethyluroniumhexafluoro phosphate (HBTU, 226.0mg, 0.596mmole), 1-
rrydroxybenzotriazolehydrate and (HOBT, 92mg, 0.596mmole) were dissolved in dimethylformamide (3mL) and N-methylmorpholine (NMM, 131 \iL, 1.197mmole) added. After pre-activation for 5 minutes, free amine gears (160 x 1.2umole) were added, followed by dimethylformamide (17.5mL) and left overnight. The spent coupling solution was then added to free amine crowns (18 x lOjamole) and left overnight. Standard washing and analyses indicated quantitative loading in both cases.
Following the general details from Scheme 2, the required loaded building block-linker construct was elaborated on the solid phase as follows:
Loaded construct was elaborated to EXAMPLE 83 (3aS, TaR) Morpholine-4-carboxylic acid [lJl?-benzylsulfanylmethyl-2-oxo-2-(3-oxohexahydrofuro[3,2-fe] pyridin-4-yl)ethyl]amide by standard Fmoc deprotection and sequential coupling with Fmoc-Cys(SBzl)-OH then morpholine-4-carbonylchloride. The crude example was cleaved and analysed (see general techniques). HPLC Rt = 15.59mins (>80%), HPLC-MS 449.2 [M + Hf.
The following examples (84 - 130) were prepared as detailed for EXAMPLE 83, coupling with the required reagents to provide the full length molecule.
HPLC Rt = 15.79mins (> 80%), HPLC-MS 459.3 [M + Hf.
EXAMPLE 84. (3aS, 7a#) Naphthalene-2-carboxylic acid [15-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3s2-6]pyridin-4-yl)ethyl]amide
EXAMPLE 85. (3aS, 7aR) 4-Dimethylamino-JV-[15-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro [3,2-&]pyridin-4-yI)ethyl]benzamide
HPLC Rt = 10.98mins (> 80%), HPLC-MS 452.2 [M + H]+.
EXAMPLE 86. (3aS, 7a#) Naphthalene-1-cafboxylic acid [3-methyl-15-(3-oxo-hexahydrofuro [3,2-6]pyridine-4-carbonyl) butyl] amide
PLC Rt = 17.71mins (> 80%), HPLC-MS 409.2 [M + Hf.
EXAMPLE 87. (3aS, 7aR) Naphthalene-2-carboxylic acid [3-methyl-15-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl)butyl]amide(Figure Remove)EXAMPLE 8. (3aS, 7a#) JV-[lS-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydro furo[3,2-
HPLC Rt = 13.16mins (> 80%), HPLC-MS 492.2 [M + Hf..ydroxyben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-b]pyridin-4-yl)ethyl]amide HPLC Rt = 17.38mins (> 80%), HPLC-MS 485.2 [M + H]+.
EXAMPLE 91. (3aS, 7a#) 4-fe^-butyl-Ar-[16'-(4-hydroxyben2yl)-2-oxo-2-(3-oxo-h.exahydrofuro[3,2-6]pyridin-4-yl)ethyl]benzamide
HPLC Rt = 17.90mins (> 80%), HPLC-MS 485.2 [M + Hf.
EXAMPLE 92. (3aS, 7aR) ^-[15-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexaliydrofuro [3^2-6]pyridin-4-yl)ethyl]-4-thiophen-2-ylbenzaniide
HPLC Rt = 17.00mins (> 80%), HPLC-MS 491.2 [M + H]+.
EXAMPLE 93. (3aS, 7aR) Ar-[l^-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-liexahydrofuro o,2-Z7]pyridin-4-yl)ethyl]-4-trifluorometlioxybenzamide
HPLC Rt = 17.07mins (> 80%), HPLC-MS 493.2 [M + H]+.
EXAMPLE 94. (3aS, 7aK) ^-[3-methyl-lS-(3-oxo-hexahydrofuro[3,2-Z)]pyridme-4-carbonyl)butyl]-5-thiophen-2-ylnicotinamide

HPLC Rt = 15.82mins (> 80%), HPLC-MS 442.2 [M + H]+.
EXAMPLE 95. (3aS, 7a£) 2-Pyridin-3-ylthiazole-4-carboxylic acid [3-methyl-15-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl) butyl]amide
HPLC Rt = 12.55niins (> 80%), HPLC-MS 443.2 [M + H]+.
EXAMPLE 96. (3aS, 7aR) Biphenyl-4-carboxylic acid [3-methyl-15-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl)butyl]amide
EXAMPLE 97. (3aS, 7aR) 4-/ert-butyl-^-[3-mefliyl-15-(3-oxo-hexahydiofuro[3,2-
6]pyridine-4-carbonyl) butyl]benzamide
HPLC Rt = 20.42mins (> 80%), HPLC-MS 415.2 [M + H]+.
EXAMPLE 98. (3aS, 7aR) JV-[3-meayl-15-(3-oxo-hexahydK)fuio[3^-&]pyridine^-carbonyl)butyl]-4-thiophen-2-ylbenzamide
(Figure Remove)HPLC Rt = 19.60mins (> 80%), HPLC-MS 441.2 [M + H]+.
EXAMPLE 99. (3aS, 7aK) 7V-[3-methyl-15'-(3-oxo-hexahydrofuro[352-i]pyridine-4-carbonyl)butyl]-4-trifluoromethoxybenzamide
(Figure Remove)-
HPLC Rt = 19.66mins (> 80%), HPLC-MS 443.2 [M + H]+.
EXAMPLE 100. (3aS; laK) 2-Methyl-5-phenylfuran-3-carboxylic acid [3-methyl-
1 iS-^-oxo-hexahydrofurop ,2-i]pyridine-4-carbonyl) butyljamide
HPLC Rt = 20.48mins (> 80%), HPLC-MS 439.2 [M + H]+.
EXAMPLE 101. (3aS, 7aR) Moipholine-4-carboxyUc acid [lS-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]amide
HPLC-MS 418.2 [M + Hf.
EXAMPLE 102. (3aS, laK) JV-[15-(4-hydroxybenzyl-2-oxo-2-(3-oxo-hexahydroforo [3,2-6]pyridin -4-yl)ethyl]benzamide
PLC Rt = 12.71mins (>85%), HPLC-MS 409.2 [M + H]+.
EXAMPLE 103. (3a5, laK) Naphthalene-1-carboxylic acid [15-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydroftjro[3,2-fe]pyridin-4-yl)ethyl]amide
HPLC Rt = 14.88mins (>85%), HPLC-MS 459.2 [M + H]+.
HPLC Rt = 11.14mins (>85%), HPLC-MS 399.2 [M + H]+.
EXAMPLE 104. (3aS, laK) Furan-3-carboxylic acid [15-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydroiuro[3,2-^]pyridin-4-yl)ethyl]amide
EXAMPLE 105. (3a5, laK) Benzo[fc]thiophene-2-carboxylic acid [15'-(4-hydroxy ben2yl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridm-4-yl)ethyl]amide
HPLC Rt = 15.99mins (>85%), HPLC-MS 465.2 [M + H]+.
EXAMPLE 106. (3aS, 7aR) Benzo[fe]thiophene-3-carboxylic acid [15-(4-hydroxy benzyl)-2-oxo-2-(3-oxo-hexah.ydrofuro[3,2-6]pyridin-4-yl)eihyl]amide
(Figure Remove)HPLC Rt = 15.58mins (>85%), HPLC-MS 465.2 [M + H]+5 951.3 [2M +Naf.
EXAMPLE 107. (3aS, laK) Furan-3-carboxylic acid [15'-benzyl-2-oxo-2-(3-oxo-hexahydrofuro[352-^]pyridin-4-yl)ethyl]amide
(Figure Remove)
HPLC Rt = 14.44mins (>85%), HPLC-MS 383.2 [M + Hf.
EXAMPLE 108. (3aS, 7 a/?) Thiophene-3-carboxylic acid [15-benzyl-2-oxo-2-(3-oxo-hexah.ydrofuro[3,2-6]pyridin-4-yl)ethyl]amide
HPLC Rt = 15.34mins (>80%), HPLC-MS 399.2 [M + Hf.
EXAMPLE 109. (3aS, 7aK) Morpholine-4-carboxylic acid [3-methyl-15-(3-oxo-hexa hydrofuro[3,2-6]pyridine-4-carbonyl)butyl]amide
HPLC-MS 368.2 [M + Hf.
EXAMPLE 110. (3aS, 7a^)^-[3-methyl-15-(3-oxo-hexahydrofuro[3,2-i]pyridine-4-carbonyl) butyl]benzamide

HPLC Rt = 15.42mins (>85%), HPLC-MS 359.2 [M + H]+.
EXAMPLE 111. (3aS, laK) Furan-3-carboxylic acid [3-methyl-15-(3-oxo-hexahydro furo [3,2-6]pyridine-4-carbonyl) butyl] amide
(Figure Remove)HPLC Rt = 13.74mins (>85%), HPLC-MS 349.2 [M + H]+.
EXAMPLE 112. (3aS, laK) Benzo[6]tbiophene-2-carboxylic acid [3-methyl-15-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl) butyl] amide
HPLC Rt = 18.52mins (>85%), HPLC-MS 415.2 [M + H]+, 851.3 [2M + Naf.
EXAMPLE 113. (3aS, 7&K) 4-Dime%larnino-Ar-[3-metliyl-15'-(3-oxo-hexahydro furo[3,2-6]pyridine-4-carbonyl)butyl]ben2amide
HPLC Rt = 13.55mins (>85%), HPLC-MS 402.2 [M + H]+.
EXAMPLE 114. (3aS, laR) Benzo[6]thiophene-3-carboxylic acid [3-methyl-lS-(3-oxo-hexahydrofuro[3,2-i]pyridine-4-carbonyl)butyl]amide
HPLC Rt = 18.26mins (>85%), HPLC-MS 415.2 [M + H]+.
EXAMPLE 115. (3aS, laR) Thiophene-3-carboxylic acid Omethyl-lS-^-oxo-hexa liydrofuro[3,2-fe]pyridine-4-carbonyl)butyl]amide
HPLC Rt = 14.80mins (>85%), HPLC-MS 365.2 [M + Hf.
EXAMPLE 116. (3aS, laR) N-[lR-ber^ls^lfanylme±y\-2-oxo-2-(3-oxo-'hexahydTO furo[3,2-Z)]pyridin-4-yl)ethyl]benzamide
HPLC Rt = 17.78mins (>90%), HPLC-MS 439.2 [M + H]+, 899.3 [2M + Na]+.
EXAMPLE 117. (3aS, 7aJ?) Furan-3-carboxylic acid-[l^-benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyridin-4-yl)ethyl]amide
HPLC Rt = 16.41mins (>85%), HPLC-MS 429.2 [M + H]+, 451.2 [M + Na]+.
EXAMPLE 118. (3aS, 7aJ?) Thiophene-3-carboxylic acid-[l/?-ben2ylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyridin-4-yl)ethyl]amide
HPLC Rt = 17.23mins (>85%), HPLC-MS 445.2 [M + H]+, 911.2 [2M + Naf.
EXAMPLE 119. (3aS, 7a/J) Morpholine-4-carboxylic acid [l.S-cyclohexylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyridin-4-carbonyl)ethyl]amide
(Figure Remove)
HPLC-MS 408.3 [M + Hf, 430.3 [M + Naf.
EXAMPLE 120. (3aS, laR) W-[lS-cyclohexylme%l-2-oxo-2-(3-oxo-hexahydrofuro [3,2-6]pyridin-4-yl)ethyl]benzamide
HPLC Rt = 18.47mins (>85%), HPLC-MS 399.2 [M + H]+, 819.2 [2M + Naf.
EXAMPLE 121. (3aS, 7aK) Furan-3-carboxylic acid [l (Figure Remove)HPLC Rt = 17.12mins (>85%), HPLC-MS 389.2 [M + Hf, 411.2 [2M + Naf.
EXAMPLE 122. (3aS, 7aK) Thiophene-3-carboxylic acid [15'-cyclohexylmethyl-2-oxo-2-(3-oxo-hexah.ydroforo[3,2-^]pyridin-4-yl)ethyl]amide
HPLC Rt = 17.95mins (>85%), HPLC-MS 405.2 [M + Hf, 831.2 [2M + Naf.
EXAMPLE 123. (3aS, 7 aft) Furan-3-carboxylic acid [15-cyclopentylmethyl-2-oxo-2-(3-oxo-hexab.ydrofuro[3,2-fo]pyridin-4-yl)ethyl]amide
HPLC Rt = 1 S.SOmins (>80%), HPLC-MS 375.2 [M + Hf, 771.3 [2M + Naf.
EXAMPLE 124. (3aS, 7aR) Thiophene-3-carboxylic acid [15-cyclopentylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]amide
HPLC Rt = 16.72mins (>85%), HPLC-MS 391.1 [M + Hf, 803.3 [2M + Naf.
EXAMPLE 125. (3aS, laR) Fioran-3-carboxylic acid [3J3-dimethyl-l1S'-(3-oxo-liexa hydrofuro[3,2-Z?]pyridine-4-carbohyl)butyl]amidePLC Rt = 15.06mins (>90%), HPLC-MS 363.2 [M + Hf, 747.4 [2M + Naf.
EXAMPLE 126. (3aS, laK) Thiophene-3-carboxylic acid [3,3-dimethyl-15'-(3-oxo-hexahydrofuro[3,2-&]pyridine-4-carbonyl) butyl]amide
HPLC Rt = 15.75mins (>85%), HPLC-MS 379.2 [M + Hf, 779.3 [2M + Naf.
EXAMPLE 127. (3aS, 7aR) Morpholine-4-carboxylic acid 15-cyclohexyhnethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-&]pyridin-4-yl)ethyl ester
HPLC-MS 409.2 [M + Hf, 839.5 [2M + Naf.
As detailed for EXAMPLE 74, compound (33) was coupled under standard conditions (following Fmoc removal) to the (3aS, laK) 3-oxo-hexahydrofuro[3,2-£]pyridine-4-carboxylic acid 9#-fluoren-9-ylmethyl ester equivalent of loaded building block-linker construct (22), then cleaved to provide EXAMPLE 127.
EXAMPLE 128. (2S, 3aS, 7aR) 4-[3-Cyclohexyl-2-(furan-2-yknethanesulfanyl) propionyl]hexahydrofuro[3,2-6]pyridin-3-one
HPLC-MS 392.1 [M + Hf, 805.2 [2M + Na]+.
As detailed for EXAMPLE 76, compound (41) was coupled under standard conditions (following Fmoc removal) to the (3aS, laR) 3-oxo-hexahydrofuro[3,2-&]pyridine-4-carboxylic acid 9.fiT-fiuoren-9-ylmetb.yl ester equivalent of loaded building block-linker construct (22), then cleaved to provide EXAMPLE 128. EXAMPLE 129. (2S, 3aS, laR) 4-[3-Cyclohexyl-2-(furan-2-yhnethanesulphonyl) propionyl]hexahydrofuro[3,2-fc]pyridin-3-one
HPLC Rt = 19.015mins (>%), HPLC-MS 424.1 [M + Hf, 869.2 [2M + Naf.
As detailed for EXAMPLE 76, compound (41) was coupled under standard conditions (following Fmoc removal) to the (3aS, laR) 3-oxo-hexahydrofuro[3,2-£»]pyridine-4-carboxylic acid 9/J-fluoren-9-ylmethyl ester equivalent of loaded building block-linker construct (22). The intermediate loaded thioether (1.2p,mole gear) was oxidised with m-chloroperbenzoic acid (5eq, 65% reagent, 1.6mg) in dichloromethane (200faL) for 5hrs, followed by standard washing and then cleaved to provide EXAMPLE 129.
EXAMPLE 130. (3aS, 7aR) 2tf-Cyclohexylmethyl-4-morpholin-4-yl-l-(3-oxo-hexa hydrofuro[3,2-&] pyridin-4-yl)butane-l ,4-dione
HPLC-MS 407.1 [M + Hf, 833.3 [2M+Naf.
As detailed for EXAMPLE 77, compound (36) was coupled under standard conditions (following Fmoc removal) to the (3aS, 7a/?) 3-oxo-hexahydrofuro[3,2-6]pyridine-4-carboxylic acid 9#-fluoren-9-ylmethyl ester equivalent of loaded building block-linker construct (22), then cleaved to provide EXAMPLE 130. EXAMPLE 131 (An example of general formula (II)). (3aR, 1aS) Morpholine-4-carboxylic acid [lJ?-benzylsulfanylmethyl-2-oxo-2-(3-oxohexahydrofuro[3,2-6]pyridin-4-yl)ethyl]amide
The required bicycle building block (3aR, laS) 3-oxo-hexahydrofuro[3,2-6]pyridine-4-carboxylic acid 977-fluoren-9-ylmethyl ester was prepared in a further 4 steps from (2R, 3S) (3-hydroxy) pyrrolidine-l,2-dicarboxylic acid 2-allyl ester l-(9H-fluoren-9-ylmethyl) ester as follows.
(1) Preparation of (2R, 3S) (3-tert-butoxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester l-(9H-fluoren-9-ylmethyl) ester.
(2R, 35) (3-hydoxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester 1-(9H-fluoren-9-ylmethyl) ester (1.75g, 4.30mmole) was dissolved in dry dichloromethane (20mL) in a 50mL glass pressure tube and cooled to -78°C. Isobutylene gas (~10mL) was condensed into the solution and cH2SO4 (100(j.L) added. A stirrer bar was added, the tube was sealed, the cooling removed and stirred at RT for 72hr. The sealed tube was cooled to -78°C, N-methylrnorpholine (200pL, leq w.r.t. cH2SO4) and allowed to warm to RT, unsealed, with stirring over 2hr. Dichloromethane (75mL) was added and the organics washed with NaHCO3 (75mL), then brine (75mL) and dried (NaiSO^. The solvents were removed in vacuo to give a pale tan oil (1.93g). The crude oil was purified over silica gel (lOOg) eluting with a gradient of heptane : ethyl acetate 5:1 •> 3:1. Desired fractions were combined and reduced in vacuo to a thick clear oil yield 1.48g (3.19mmole, 74.3%). TLC (single UV spot, Rf = 0.50, heptane: ethyl acetate 2:1), analytical HPLC Rt = 24.083mins, HPLC-MS (single main UV peak with Rt = 11.996mins, 408.2 [M + H-Bu'f, 486.3 [M + Naf). Analysis by *H and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond. 8H (CDC13 at 298K); 1.24 (9 x C(CH3)3, s), 1.51-1.90 (2Hy+ 2H8, m), 3.38-3.48/3.49-3.58 (lHe, dt), 3.62-3.74 (Hp, b), 3.95-4.02/4.06-4.14 (1HE, dd), 4.24-4.78 (Fmoc H-9 + Fmoc CH^ + 2 x COOCH2CH=CH2, bm), 4.88/5.01 (Ha, dd, J5.8), 5.25 (1 x COOCH2CH=CH2, d, J5.7), 5.37 (1 x COOCH2CH=CH2, d, J16.8), 5.88-6.00 (1 x COOCH2CH=CH2, m), 7.28-7.39 (2H aromatic, Fmoc H-2 and H-7), 7.39-7.46 (2H aromatic, Fmoc H-3 and H-6), 7.57-7.71 (2H aromatic, Fmoc H-l and H-8), 7.78-7.86 (2H aromatic, Fmoc H-4 and H-5).
5C (CDC13 at 298K); 23.69/24.00 (d, C5), 28.38 (u, C(CH3)3), 28.65 (d, Cy), 40.41/40.72 (d, Ce), 47.61 (u, Fmoc C-9), 58.46 (u, Ca), 65.67 (d, COOCH2CH=CH2), 67.82 (u, Cp), 68.12 (d, Fmoc CH2), 74.93 (q, C(CH3)3), 118.46/118.57 (d, COOCH2CH=CH2), 120.40 (u, Fmoc C-4 and C-5), 125.39 (u, Fmoc C-l and C-8), 127.46 (u, Fmoc C-2 and C-7), 128.10 (u, Fmoc C-3
and C-6), 132.49 (u, COOCH2CH=CH2), 141.71 (q, Fmoc C-4' and C-5'), 144.12/144.35 (q, Fmoc C-l' and C-8'), 156.24 (q, OCON), 170.42/171.06 (COOCH2CH=CH2).
(2) Preparation of (2R, 35) (3-ter^butoxy)pyrrolidine-l,2-dicarboxylic acid 1-(9H-fhioren-9-ylmethyl) ester.
(27?,3S) (3-ter^butoxy)pyrrolidine-l,2-dicarboxylic acid 2-allyl ester 1-(9H-fluoren-9-ylmethyl) ester (1.42g, 3.07mmole) was dissolved in dry dichloromethane (25mL) with stirring. TetraMstriphenylphosphine palladium (0) (71mg, 0.062mmole, 0.02eq) was added, followed by phenyltrihydrosilane (0.66g, 0.581mL, 6.14mmole, 2eq). After Ihr, dichloromethane (150mL) was added and the organics washed with 0.01N HC1 (150mL), brine (150mL) and dried (Na2SO4). The solvents were removed in vacuo to give a dark grey solid (2.0g). The crude solid was purified over silica gel (75g) eluting with a gradient of heptane : ethyl acetate 2:1 -^ 1:2. Desired fractions were combined and reduced in vacuo to a white crystalline yield 1.07g (2.53mmoles 82.3%). TLC (single UV spot, Rf = 0.25, heptane : ethyl acetate 1:1), analytical HPLC Rt = 21.306mins, HPLC-MS (single main UV peak with Rt = 10.269mins5 368.2 [M + H-Bu'f, 446.2 [M + Naf, 869.3 [2M + Naf). Analysis by *H and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond. sH (CDC13 at 298K); 1.32/1.41 (9 x C(CH3)3, ds), 1.43-1.59 (1HY+ 1H5, m), 1.70-1.81 (1H8, bm), 1.82-1.99 (1HY, bm), 2.75-2.82/2.90-2.97 (!He, dt), 3.73-3.85 (Hp, b), 3.92-4.05 (lHe, dd), 4.23-4.32 (Fmoc H-9), 4.40-4.58 (Fmoc CH2, bm), 4.60-4.62/5.02-5.04 (Ha, dd, J5.7), 7.29-7.36 (2H aromatic, Fmoc H-2 and H-7), 7.39-7.44 (2H aromatic, Fmoc H-3 and H-6), 7.54-7.67 (2H aromatic, Fmoc H-l and H-8), 7.77-7.82 (2H aromatic, Fmoc H-4 and H-5), 10.80-11.15 (COOH,bs).
5C (CDC13 at 298K); 23.71/24.24 (d, C5), 28.20/28.27 (u, C(CH3)3), 30.60/30.73 (d, CY), 40.50/40.79 (d, CE), 47.58/47.63 (u, Fmoc C-9), 58.20/58.44 (u, Ca), 67.77/68.33 (u, Cp), 68.67/68.87 (d, Fmoc CH2), 78.48/78.67 (q, C(CH3)3), 120.30 (u, Fmoc C-4 and C-5);
125.16/125.27/125.52 (u, Fmoc C-l and C-8), 127.42/127.48/127.59 (u, Fmoc C-2 and C-7), 128.04/128.10 (u, Fmoc C-3 and C-6), 141.57/141.69/141.78 (q, Fmoc C-4' and C-51), 144.06/144.11/144.36/144.47 (q, Fmoc C-l' and C-8'), 155.73/156.49 (q, OCON), 169.26/169.55 (COOH).
(3) Preparation of (2R, 35) (3-tert-butoxy)-2-(2-diazoacetyl)pyrrolidine-l,2-dicarboxylic acid l-(9H-fluoren-9-ylmethyl) ester.
(2R, 3S) (3-te7t-butoxy)pyrrolidine-l,2-dicarboxylic acid l-(9H-fluoren~9-ylmethyl) ester (830mg, 1.96mmole) was dissolved with stirring in dry dichloromethane (20mL). The reaction was flushed with nitrogen and cooled to -15°C. Isobutylchloroformate (296mg, 2.16mrnole in dry dichloromethane (2.5mL) and N-methylmorpholine (397mg, 3.92mmole in dry dichloromethane (2.5mL) were added simultaneously in O.SmL aliquots over ISmins. Etheral diazomethane (generated from diazald (2.5g, ~8mmole in diethyl ether (40mL)) onto sodium hydroxide (2.75g) in water (4.3mL) / ethanol (8.6mL) at 60°C). was added to the activated aminoacid solution and stirred at RT for 3hr, Acetic acid (~3mL) was added to quench the reaction, then terNbutylmeraylether (lOOmL) was added, the organics washed with water (3 x 150mL), then dried (NaaSCU). The solvents were removed in vacuo to give a tacky pale yellow oil (910mg). The crude oil was purified over silica gel (75g) eluting with a gradient of heptane : ethyl acetate 4:1 -^ 3:1. Desired fractions were combined and reduced in vacuo to a pale yellow oil/solid, yield 560mg (1.25mmole, 63.9%). TLC (single main UV spot, Rf= 0.35, heptane : ethyl acetate 2:1), analytical HPLC Rt = 18.754min, HPLC-MS (UV peaks with Rt = 9.095mins, 364.2 [M + H]+, 386.1 [M + Naf, 749.2 [2M + Naf and Rt = 10.856mins, 420.2 [M + H-N2]+5 470.2 [M + Naf, 917.3 [2M + Naf).
Note that upon HPLC-MS analysis, m/z 364.2 corresponds to the desired bicycle product (3a#, 7aS) 3-oxo-hexahydrofuro[3,2-6]pyridine-4-carboxylic acid 9#-fluoren-9-ylmethyl ester.
Analysis by 1H and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond.
5H (CDC13 at 298K); 1.29/1.46 (9 x C(CH3)3, ds), 1.40-1.85 (2HY+ 2H55bm), 3.05/3.38 (1HE, dt), 3.39-3.44/3.62-3.69 (Hp, dm), 3.90-3.97 (1HE) dd), 4.05-4.07/4.78-4.83 (Ha, bin), 4.24-4.33 (Fmoc H-9), 4.36-4.42/4.46-4.51 (1 x Fmoc CH2, dm), 4.61-4.68 (1 x Fmoc CFjb, m), 4.89/5.90 (COCH=N2, s + bs), 7.28-7.39 (2H aromatic, Fmoc H-2 and H-7), 7.40-7.44 (2H aromatic, Fmoc H-3 and H-6), 7.53-7.67 (2H aromatic, Fmoc H-l and H-8), 7.76-7.81 (2H aromatic, Fmoc H-4 and H-5).
5C (CDC13 at 298K); 23.94/24.51 (d, C5), 28.38/28.53 (u, C(CH3)3), 29.23/29.84 (d, CY), 40.83/41.45 (d, CE), 47.57/47.64 (u, Fmoc C-9), 55.50/56.38 (u, COCH=N2), 61.69/62.66 (u, Ca), 66.85/68.20 (d, Fmoc CH2), 69.35/69.61 (u, Cp), 75.11/75.54 (q, C(CH3)3), 120.29/120.32 (u, Fmoc C-4 and C-5), 124.85/125.25/125.53 (u, Fmoc C-l and C-8), 127.46/127.59 (u, Fmoc C-2 and C-7), 127.97/128.03/128.07 (u, Fmoc C-3 and C-6), 141.62/141.69/141.73 (q, Fmoc C-4' and C-5'), 144.24/144.31/144.50/144.61 (q, Fmoc C-l' and C-8'), 155.68/156.89 (q, OCON), 191.90/192.37 (COCH=N2).
(4) Cyclisation to (3aJ?, 7aS) 3-oxo-hexahydrofuro[3,2-i]pyridine-4-carboxy lie acid 9/f-fluoren-9-ylmethyl ester.
A solution of lithium chloride (494mg, 11.6mmole) in water (3mL) and acetic acid (12mL) was added to (2R, 35) (3-tert-butoxy)-2-(2-diazoacetyl) pyrrolidine-l,2-dicarboxylic acid l-(9H-fluoren-9-ylmethyl) ester (520mg, 1.16mmole). Gas was evolved and the yellow oily solid dissolved over Ihr to give a virtually colourless solution. After 90mins, chloroform (150mL) was added and the organics washed with NaHC03 (2 x 150mL), brine (150mL) and dried (Na2SO4). The solvents were removed in vacuo to give a white crystalline solid (390mg). The crude solid was purified over silica gel (75g) eluting with a gradient of heptane : ethyl acetate 3:1 -> 2:1. Desired fractions were combined and reduced in vacuo to a white crystalline solid, yield 340mg (0.94mmole, 47.7% from starting acid). TLC (single UV spot, Rf = 0.25, heptane : ethyl acetate 2:1), analytical HPLC Rt = 18.563mins, HPLC-MS
(single UV peak with Rt = 9.074mins, 364.2 [M + H]+, 386.2 [M + Naf). Analysis by JH and 13C NMR showed the presence of cis and trans geometrical isomers around the 3° amide bond.
5H (CDC13 at 298K); 1.20-1.38 (1HY+ 1HS) m), 1.51-1,68 (1HS, bm), 1.96-2.08 (1HY, brn)} 2.40-2.52/2.53-2.67 (1HE, m), 3.87 (COCHjA, d, J16.5), 3.85-3.95 (IHe, b), 4.09 (Fmoc H-9, bt), 4.14 (Hp,b), 4.21-4.46 (Fmoc CHz + COCH2B, bm), 4.62-4.70/5.00-5.09 (Ha, 2 x b), 7.23-7.27 (2H aromatic, Fmoc H-2 and H-7), 7.32-7.35 (2H aromatic, Fmoc H-3 and H-6), 7.41-7.53 (2H aromatic, Fmoc H-l and H-8), 7.69-7.71 (2H aromatic, Fmoc H-4 and H-5).
8C (CDC13 at 298K); 22.08 (d, C5), 26.61 (d, C^), 41.67 (d, CE), 47.58 (u, Fmoc C-9), 60.54 (u, Ca), 67.55 (d, Fmoc CH^, 68.40 (d, COCH2O), 72.61 (u, Cp), 120.39 (u, Fmoc C-4 and C-5), 125.42 (u, Fmoc C-l and C-8), 127.51 (u, Fmoc C-2 and C-7), 128.14 (u, Fmoc C-3 and C-6), 141.73 (q, Fmoc C-4' and C-5'), 144.16 (q, Fmoc C-l' and C-8'), 156.20 (q, OCON), 211.64 (q, COCH20).
Following the general details from Scheme 2, the required bicycle building block (3a#, 7aS) 3-oxo-hexahydroforo[3^-6]pyridine-4-carboxylic acid 9#-fluoren-9-yl methyl ester was converted to building block-linker construct as follows:
(3a#, 7a5) 3-oxo-hexahydrofuro[3,2-6]pyridine-4-carboxylic acid 9#-fluoren-9-ylmethyl ester (290mg, O.SOmmole) was dissolved in a mixture of ethanol (14.0mL) and water (2.0mL) containing sodium acetate.trihydrate (163mg, 1.198mmole, l.Seq). 4-[[(hydrazinocarbonyl)amino]methyl]cyclo hexanecarboxylic acid. trifluoroacetate (263mg, O.SOmmole, l.Oeq, Murphy, A. M. et al, J. Am. Chem, Soc., 114, 3156-3157, 1992) was added and the mixture refluxed for 4hrs. Chloroform (150mL) was added and the organics washed with HC1 (150mL, ~ pH3), dried (Na2S04) and reduced in vacuo to provide crude building block-linker construct as an off-white crystalline solid. Yield 450mg, analytical HPLC 2 peaks Rt = 17.109 (67.6%) and 18.565mins (26.3%) (cis / trans geometrical isomers), HPLC-MS (2 x
mins, 561.2 [M + Hf). Crude construct was used directly for construct loading.
Following the general details from Scheme 2, the required building block-linker construct was attached to the solid phase providing loaded building block-linker construct as follows:
Building block-linker construct (0.680mmoles), 2-(lH-benzotriazole-l-yl)-l, 1,3,3-tetramethyluroniumhexafluoro phosphate (HBTU, 258mg, 0.680mmole), 1-hydroxybenzotriazole.hydrate and (HOBT, lOSmg, 0.680mmole) were dissolved in dimethylformamide (3mL) and N-methylmorpholine (NMM, ISOjiL, 1.36mmole) added. After pre-activation for 5 minutes, free amine gears (190 x 1.2umole) were added, followed by dimethylformamide (17.5mL) and left overnight. The spent coupling solution was then added to free amine crowns (18 x lOfimole) and left overnight. Standard washing and analyses indicated quantitative loading in both cases.
Following the general details from Scheme 2, the required loaded building block-linker construct was elaborated on the solid phase as follows:
Loaded construct was elaborated to EXAMPLE 91 (3>aR, laS) Morpholine-4-carboxylic acid [lJ?-benzylsulfanyhnethyl-2-oxo-2-(3-oxohexahydrofuro[3,2-Z)] pyridin-4-yl)ethyl]amide by standard Fmoc deprotection and sequential coupling with Fmoc-Cys(SBzl)-OH then morpholine-4-carbonylchloride. The crude example was cleaved and analysed (see general techniques). HPLC Rt = 15.59mins (>80%), HPLC-MS 449.2 [M + Hf.
The following examples (132 - 156) were prepared as detailed for EXAMPLE 131, coupling with the required reagents to provide the full length molecule.
EXAMPLE 132. (3a#, 7aS) Morpholine-4-carboxylic acid [lS-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydrofuro[3,2-b]pyridin-4-yl)ethyl]amide
(Figure Remove)HPLC-MS 418.2[M + Hf.
EXAMPLE 133. (3aR, 7aS) J\r-[l.S-(4-hydroxybenzyl-2-oxo-2-(3-oxo-hexahydroriiro [3,2-fc]pyridin-4-yl)ethyl]benzamide
HPLC Rt = 12.67mins (>80%), HPLC-MS 409.1 [M + H]+.
EXAMPLE 134. (3a#, 7aS) Naphthalene-1-carboxylic acid [15-(4-hydroxybenzyl)-2-oxo-2-(3-oxo-hexahydroruro[3,2-6]pyridin-4-yl)ethyl]amide
(Figure Remove)HPLC Rt = 14.87mins (>80%), HPLC-MS 459.2 [M + H]+.
EXAMPLE 135. (3a/?, 7aS) Morpholine-4-carboxylic acid [3-methyl-15'-(3-oxo-liexa hydrofuro[3,2-Z?]pyridine-4-carbonyl)butyl]arnide
HPLC-MS 368.2 [M + H]+, 390.2 [M + Naf.
EXAMPLE 136. (3aR, laS) ^-[3-methyl-15-(3-oxo-hexahydrofuro[3,2-fe]pyridine-4-carbonyl) butyl]benzamide
(Figure Remove)HPLC Rt = 15.40mins (>80%), HPLC-MS 359.2 [M + H]+.
EXAMPLE 137. (3aR, 7aS) Furan-3-carboxylic acid [3-methyl-15-(3-oxo-hexahydro furo[3,2-6]pyridine-4-carbonyl)butyl]amide

HPLC Rt = 13.74mins (>85%), HPLC-MS 349.2 [M + Hf, 371.2 [2M + Naf.
EXAMPLE 138. (3aR, laS) Benzo[&]tbiophene-2-carboxylic acid [3-methyl-15-(3-oxo-hexahydrofuro[3,2-6]pyridine-4-carbonyl) butyl]amide
(Figure Remove)HPLC Rt = 18.52mins (>80%), HPLC-MS 415.2 [M + H]+, 851.3 [2M + Naf.
EXAMPLE 139. (3aR, laS) 4-Dimethylamino-^-[3-inethyl-15-(3-oxo-hexahydro furo[3,2-fo]pyridine-4-carbonyl)butyl]benzamide
HPLC Rt = 13.55mins (>80%), HPLC-MS 402.2 [M + Hf, 825.3 [2M + Naf.
EXAMPLE 140. (3a#, 7aS) Benzo[6]thiophene-3-carboxylic acid [3-methyl-l,S'-(3-oxo-hexahydrofuro[3,2-6]pyridme-4-carbonyl)butyl]amide
HPLC Rt = 18.26mins (>80%), HPLC-MS 415.2 [M + Hf, 851.3 [2M + Naf.
EXAMPLE 141. (3a#, 7aS) Thiophene-3-carboxylic acid [3-methyl-lS-(3-oxo-hexa hydroforo[3,2-6]pyridine-4-carbonyl)butyl]amide
(Figure Remove)HPLC Rt = 14.80mins (>80%), HPLC-MS 365.2 [M + Hf, 387.2 [M + Naf.
EXAMPLE 142. (3aR, 7a5) N-[l^-benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydro furo [3,2-6]pyridin-4-yl)ethyl]benzamide
HPLC Rt = 17.78mins (>80%), HPLC-MS 439.2 [M + H]+, 899.3 [2M + Naf.
EXAMPLE 143. (3a/?, 7aS) Furan-3-carboxylic acid-tl.R-benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyridin-4-yl)ethyl]amide-
HPLC Rt = 16.41mins (>85%), HPLC-MS 429.6 [M + H]+, 451.5 [M + Naf.
EXAMPLE 144. (3aR, laS) Thiophene-3-carboxylic acid-fljR-benzylsulfanylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]amide

HPLC Rt = 17.23mins (>SO%), HPLC-MS 445.1 [M + Hf, 91 1.2 [2M + Naf.
EXAMPLE 145. (3a^, 7aS) Morpholine-4-carboxylic acid [15-cyclohexylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)e1:hyl]ainide
HPLC-MS 408.2 [M + Hf.
EXAMPLE 146. (3a#, 7aS) 7/-[l,S-cyclohexylmethyl-2-oxo-2-(3-oxo-hexaliydrofiiro [3,2-6]pyridin-4-yl)ethyl]benzamide
HPLC Rt = 18.47mins (>80%), HPLC-MS 399.2 [M + H]+, 819.3 [2M +Na]+.
EXAMPLE 147. (3aK, 7aS) Furan-3-carboxylic acid [15'-cyclohexylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]amide
HPLC Rt = 17.12mins (>80%), HPLC-MS 389.2 [M + Hf.
EXAMPLE 148. (3a#, 7aS) Thiophene-3-carboxylic acid [15-cyclohexylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-6]pyridin-4-yl)ethyl]arnide
HPLC Rt = 18.05mins (>85%), HPLC-MS 405.2 [M + H]+, 831.2 [2M +Naf.
EXAMPLE 149. (3aR, 7aS) Furan-3-carboxylic acid [15-cyclopentylmethyl-2-oxo-2-(3-oxo-hexahydrofaro[3J2-ft]pyridin-4-yl)ethyl]amide
HPLC Rt = 15.84mins (>85%), HPLC-MS 375.2 [M + Hf, 771.3 [2M +Naf.
EXAMPLE 150. (3aR, laS) Thiophene-3-carboxylic acid [15-cyclopentylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-fe]pyridin-4-yl)ethyl]amide
HPLC Rt = 16.78mins (>85%), HPLC-MS 391.1 [M + Hf, 803.3 [2M
EXAMPLE 151. (3aR, laS) Furan-3-carboxylic acid [3,3-dimethyl-l.Sl-(3-oxo-liexa hydrofuro[3,2-6]pyridine-4-carbonyl)butyl]amide
HPLC Rt = 15.05mins (>85%), HPLC-MS 363.2 [M + Hf, 747.4 [2M + Naf.
EXAMPLE 152. (3a#, 7aS) Thiophene-3-carboxylic acid [3,3-dim.ethyl-15-(3-oxo-hexahy droftiro [3,2-Z?]pyridine-4- carbonyl) butyl] amide
HPLC Rt = 15.76mins (>80%)3 HPLC-MS 379.2 [M + H]+, 779.3 [2M + Naf.
EXAMPLE 153. (3aR, 7aS) Morpholine-4-cafboxylic acid lS-cyclohexylmethyl-2-oxo-2-(3-oxo-hexahydrofuro[3,2-i]pyridin-4-yl)ethyl ester
HPLC-MS 409.2 [M + Hf, 839.5 [2M + Naf.
As detailed for EXAMPLE 127, compound (33) was coupled under standard conditions (following Fmoc removal) to the (3aR, 7aS) 3-oxo-hexahydrofuro[3,2-6]pyridine-4-carboxylic acid 9//'-fluoren-9-ylmethyl ester equivalent of loaded building block-linker construct (22), then cleaved to provide EXAMPLE 153.
EXAMPLE 154 (25, 3aR, laS) 4-[3-Cyclohexyl-2-(furan-2-ylmethanesulfanyl) propionyl]hexahydrofuro[3,2-6]pvridin-3-one
HPLC-MS 392.1 [M + H]+, 805.3 [2M + Naf.
As detailed for EXAMPLE 128, compound (41) was coupled under standard conditions (following Fmoc removal) to the (3aft, 7aS) 3-oxo-hexahydrofuro[3,2-
&]pyridine-4-carboxylic acid 9Muoren-9-ylmethyl ester equivalent of loaded building block-linker construct (22), then cleaved to provide EXAMPLE 154.
EXAMPLE 155. (25, 3aR, laS) 4-[3-Cyclohexyl-2-(furan-2-ylmethanesulphonyl) propionyl]hexahydrofuro[3,2-&]pyridin-3 -one
HPLC Rt = 18.8 - 19.4mins (>90%), HPLC-MS 424.1 [M + Hf, 869.2 [2M + Naf.
As detailed for EXAMPLE 129, compound (41) was coupled under standard conditions (following Fmoc removal) to the (3aR, 7aS) 3-oxo-hexahydrofuro[3,2-i]pyridine-4-carboxylic acid 9#-fluoren-9-ylmethyl ester equivalent of loaded building block-linker construct (22). The intermediate loaded thioethef (1.2jamole gear) was oxidised with m-chloroperbenzoic acid (5eq, 65% reagent, 1.6mg) in dichloromethane (200uL) for 5hrs, followed by standard washing and then cleaved to provide EXAMPLE 155.
HPLC-MS 407.2 [M + Hf, 833.3 [2M + Naf.
EXAMPLE 156. (3aR, laS) 2J?-Cyclohexyhnethyl-4-morpholin-4-yl-l-(3-oxo-hexa hydrofuro[3,2-6] pyridin-4-yl)butane-l ,4-dione
As detailed for EXAMPLE 130, compound (36) was coupled under standard conditions (following Fmoc removal) to the (3aR, 7aS) 3-oxo-hexahydrofuro[3,2-&]pyridine-4-carboxylic acid 9#-fluoren-9-ylmethyl ester equivalent of loaded building block-linker construct (22), then cleaved to provide EXAMPLE 156.
EXAMPLE A. Assays for Cysteine Protease Activity
The compounds of this invention may be tested in one of a number of literature based biochemical assays that are designed to elucidate the characteristics of compound inhibition. The data from these types of assays enables compound potency and the rates of reaction to be measured and quantified. This information, either alone or in combination with other information, would allow the amount of compound required to produce a given pharmacological effect to be determined.
General materials and methods
Unless otherwise stated, all general chemicals and biochemicals were purchased from either the Sigma Chemical Company, Poole, Dorset, U.K. or from Fisher Scientific UK, Loughborough, Leicestershire, U.K. Absorbance assays were carried out in flat-bottomed 96-well plates (Spectra; Greiner Bio-One Ltd., Stonehouse, Gloucestershire, U.K.) using a SpectraMax PLUS384 plate reader (Molecular Devices, Crawley, U.K.). Fluorescence high throughput assays were carried out in either 384-well microtitre plates (Corning Costar 3705 plates, Fisher Scientific) or 96-well CU' bottomed Microfluor Wl microtitre plates (Thermo Labsystems, Ashford, Middlesex, U.K.). Fluorescence assays were monitored using a SpectraMax Gemini fluorescence plate reader (Molecular Devices). For substrates employing either a 7-amino-4-methylcoumarin (AMC) or a 7-amino-4-nifluoromethylcoumarin (AFC) fluorophore, assays were monitored at an excitation wavelength of 365 nm and an emission wavelength of 450 nm and the fluorescence plate reader calibrated with AMC. For substrates employing a 3-amino-benzoyl (Abz) fluorophore, assays were monitored at an excitation wavelength of 310 nm and an emission wavelength of 445 nm; the fluorescence plate reader calibrated with 3-amino-benzamide (Fluka). Unless otherwise indicated, all the peptidase substrates
were purchased from Bachem UK, St. Helens, Merseyside, UK. Substrates utilizing fluorescence resonance energy transfer methodology (i.e. FRET-based substrates) were synthesized at Incenta Limited using published methods (Atherton & Sheppard, Solid Phase Peptide Synthesis, IRL Press, Oxford, U.K., 1989) and employed Abz (2-aminobenzoyl) as the fluorescence donor and 3-nitro-tyrosine [TyrCNOa)] as the fluorescence quencher (Meldal, M. and Breddam, K.,Anal. Biochem., 195,141-147, 1991). Hydroxyethylpiperazine ethanesulfonate (HEPES), tris-hydroxylro ethyl aminomethane (tris) base, bis-tris-propane and all the biological detergents (e.g. CHAPS, zwittergents, etc.) were purchased from CN Biosciences UK, Beeston, Nottinghamshire, U.K. Glycerol was purchased from Amersham Pharmacia Biotech, Little Chalfont, Buckinghamshire, U.K. Stock solutions of substrate or inhibitor were made up to 10 mM in 100 % dimethylsulfoxide (DMSO) (Rathburns, Glasgow, U.K.) and diluted as appropriately required. In all cases the DMSO concentration in the assays was maintained at less than 1% (vol./vol.).
Assay protocols were based on literature precedent (Tablel; Barrett, A.J., Rawlings, N.D. and Woessner, J.F., 1998, Handbook of Proteolytic Enzymes, Academic Press, London and references therein) and modified as required to suit local assay protocols. Enzyme was added as required to initiate the reaction and the activity, as judged by the change in fluorescence upon conversion of substrate to product, was monitored over time. All assays were carried out at 25±1°C.
Table 1. The enzyme assays described herein were earned out according to literature precedents.

I: 10 mM BTP, pH 6.5 containing 1 mM EDTA, 5 mM 2-mercaptoethanol and 1 mM
CaCl2 H: 10 mM BTP, pH 6.5 containing 1 mM EDTA, 142 mM NaCl, 1 mM DTT, 1 mM
CaCl2, 0.035 mM Zwittergent 3-16 III: 50mM HEPES pH 7.2, 10% Glycerol, 0.1% CHAPS, 142 mM NaCl, 1 mM
EDTA, 5 mM DTT TV: 100 mM sodium phosphate, pH 6.75 containing 1 mM EDTA and 10 mM L-
cysteine
V: 50 mM trisacetate, pH 8.4 containing 1 mM EDTA, 10 mM L-cysteine and
0.25% (w/v) CHAPS
VI: 10 mM HEPES, pH 8.0 containing 5 mM CaCl2
VII: 10 mM HEPES, pH 7.5 containing 2 mM 2-mercaptoethanol and 100 p,M CaCl2 VHI: 10 mM HEPES, pH 7.5 containing 2 mM 2-mercaptoethanol and 200 uM
CaCl2
EX: 100 mM sodium acetate; pH 5.5 containing 10 mM L-cysteine and 1 mM EDTA X: 100 mM sodium acetate; pH 5.5 containing 10 mM L-cysteine; 0.05% (w/v) Brij
35 and 1 mM EDTA
XI: 100 mM sodium acetate; pH 5.5 containing 10 mM L-cysteine; 142 mM sodium chloride and 1 mM EDTA
a Barrett, A.J., Biochem. J., 187,909-912,1980
b Barrett, AJ. and Kirschke, H., Methods Erizymol, 80, 535-561,1981
0 Quibell, M. and Taylor, S.5 WO0069855, 2000
dBromme, D., Steinert,., Freibe, S., Fittkau, S., Wiederanders, B., and Kirschke, H.,
Biochem. J., 264,475-481,1989 °Rano, T.A., et. al.Chem. Biol, 4,149,1997 fTalanian, R.V., et. al, J. Biol. Chem., 272, 9677,1997 8 Lazebnik, Y.A., Kaufinann, S.H., Desnoyers, S., Poirer, G.G. and Earnshaw, W.C.,
Nature., 371, 768-774,1994 hHan, Z., et. al., J. Biol. Chem., 272,13432,1997
1 Takahashi, A., et. al, PNAS, 93, 8395,1996
j Martins, L.M., et. al, J. Biol Chem., 272, 7421,1997
k Nagata, S., Cell, 88,355,1997
1 Harris, J.L., et. al, J. Biol. Chem., 273,27364,1998
m Cazzulo, J.J., Cazzulo Franke, M.C., Martinez, J. and Franke de Cazzulo, B.M.,
Biochim. Biophys. Acta., 1037.186-191,1990 n Cazzulo, J.J., Bravo, M., Raimondi, A., Engstrom, U., Lindeberg, G. and Hellman,
U., CellMol Biol, 42, 691-696,1996 0 Potempa, J., Dubin, A., Korzus, G. and Travis, J., Biochem. J., 263, 2664-2667,
1988
p Kembhavi, A.A., Buttle, D J., Rauber, P. and Barrett, A.J., FEES Lett., 283, 277-
280,1991
q Alves, L.C., et. al.,Mol Biochem. Parasitol, 116,1-9, 2001. r Guarne, et.al, J. Mol. BioL, 302,1227-1240,2000. 5 Halfon and Craik, (Barret, Rawlings and Woessner, eds.), in Handbook of
Proteolytic Enzymes, Academic Press, London, 12-21,1998. 1 Sasaki, et. al, (1984), J. BioL Chem., 259, 12489-12494,1984. "Bossard, M.J., et. al.,,J. Biol. Chem., 21,12517-12524,1996 v Santamaria, L, et. al., J. Biol. Chem., 273,16816-16823,1998 w Klemencic, J, et al, Eur.J.Biochem., 267,5404-5412,2000
Tiypanosoma cnizi cruzipain peptidase activity assays
Wild-type cruzipain, derived from Trypanosoma ciiizi Dm28 epimastigotes, was obtained from Dr. Julio Scharfstein (Institute de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil). Activity assays were carried out in 100 mM sodium phosphate, pH 6.75 containing 1 mM EDTA and 10 mM L-cysteine using 2.5 nM enzyme. Ac-Phe-Arg-AMC (^Mapp ~ 12 uM) and D-Val-Leu-Lys-AMC (K^ ~ 4 uM) were used as the substrates. Routinely, Ac-FR-AMC was used at a concentration equivalent to jKMapp and D-Val-Leu-Lys-AMC was used at a concentration of 25 uM. The rate of conversion of substrate to product was derived from the slope of the increase in fluorescence monitored continuously over time.
Leishmania mexicana cysteine protease B (CPB) peptidase activity assays
Wild-type recombinant CPB without the C-terminal extention (i.e. CPB2.8ACTE; Sanderson, S.J., et. al., Biochem. J., 347, 383-388, 2000) was obtained from Dr. Jeremy Mottram (Wellcome Centre for Molecular Parasitology, The Anderson College, University of Glasgow, Glasgow, U.K.). Activity assays were carried out in 100 mM sodium acetate; pH 5.5 containing 1 mM EDTA; 200 mM NaCl and 10 mM DTT (Alves, L.C., et. al, Mol. Biochem. Parasitol, 116, 1-9, 2001) using 0.25 nM enzyme. Pro-Phe-Arg-AMC (£Mapp ~ 38uM) was used as the substrate at a
concentration equivalent to JCMapp. The rate of conversion of substrate to product was derived from the slope of the increase in fluorescence monitored continuously over time,
Cathepsin peptidase activity assays
Bovine cathepsin S, human cathepsin L, human cathepsin H and human cathepsin B were obtained from CN Biosciences. Reconibinant human cathepsin S, human cathepsin K and human cathepsin X were obtained from Dr. Boris Turk (Josef Stefan Institute, Ljubljana, Slovenia). Unless otherwise stated, all peptidase activity assays were carried out in 10 mM bis-tris-propane (BTP), pH 6.5 containing 1 mM EDTA, 5 mM 2-mercaptoethanol and 1 mM CaCl2. Human cathepsin H activity assays were carried out in 10 mM BTP pH 6.5, 142 mM NaCl2, 1 mM CaCl2> 1 mM EDTA, 1 mM DTT, 0.035 mM Zwittergent 3-16, Human cathepsin K assays were carried out in 100 mM sodium acetate; pH 5.5 containing 20 mM L-cysteine and 1 mM EDTA (Bossard, M.J., et. a!., J. Biol Chem., 2_i, 12517-12524,1996). Human cathepsin X assays were carried out in 100 mM sodium acetate; pH 5.5 containing 20 mM L-cysteine; 0.05% (w/v) Brij 35 and 1 mM EDTA (Santamaria, I., et. a/., /. Biol Chem., 273, 16816-16823,1998; Klemencic, J, et aL, Eur. J. Biochem., 267, 5404-5412, 2000). The final enzyme concentrations used in the assays were 0.5 nM bovine cathepsin S, 1 nM cathepsin L, 0.1 nM cathepsin B, 0.25nM Cathepsin K; 1 nM cathepsin X and 10 nM cathepsin H. For the inhibition assays, the substrates used for cathepsin S, cathepsin L, cathepsin B, cathepsin K and cathepsin H were boc-Val-Leu-Lys-AMC (^Mapp « 30 nM), Ac-Phe-Arg-AMC (ATMapp « 20 uM), Z-Phe-Arg-AMC (£Mapp « 40 uM), Z-Leu-Arg-AMC (KMapp ~ 2uM); Bz-Phe-Val-Arg-AMC (&Mapp ~ 150 uM) respectively. In each case the substrate concentration used in each assay was equivalent to the KM&PP- The rate of conversion of substrate to product was derived from the slope of the increase in fluorescence monitored continuously over time.
Trypsin peptidase activity assays
Human pancreatic trypsin (iodination grade; CN Biosciences) activity assays were carried out in 10 mM HEPES, pH 8.0 containing 5 mM CaCl2 using 0.1 nM trypsin. For the inhibition assays, Z-Gly-Gly-Arg-AMC (^Mapp ~ 84 pM) was used as the substrate at a concentration equivalent to KM*PP. The rate of conversion of substrate to product was derived from the slope of the increase in fluorescence monitored continuously over time.
Clostripain peptidase activity assays
Clostripain (Sigma) activity assays were carried out in 10 mM BTP, pH 6.5 containing 1 mM EDTA, 5 mM 2-mercaptoethanol and ImM CaQ2 using 0.3 nM enzyme. For the inhibition assays, Z-Gly-Gly-Arg-AMC CKMBPP ~ 100 pM) was used as the substrate at a concentration equivalent to XMSPP. The rate of conversion of substrate to product was derived from the slope of the increase in fluorescence monitored continuously over time.
Calpain peptidase activity assays
Calpain (human erythrocyte p,-calpain and porcine kidney m-calpain; CN Biosciences) activity assays were carried out in 10 mM HEPES, pH 7.5 containing 2 mM 2-mercaptoethanol and CaQ2 using 25 nM of either enzyme (Sasaki, et. al, J. Biol. Chem., 259, 12489-12494, 1984). For n-calpain inhibition assays, the buffer contained 100 uM CaCl2 and Abz-Ala-Asn-Leu-Gly-Arg-Pro-Ala-Leu-Tyr(NO2> Asp-NH2 (KMPP ~ 20 H.M; Incenta Limited) was used as the substrate. For m-calpain inhibition assays, the assay buffer contained 200 \iM CaCl2 and Abz-Lys-Leu-Cys(Bzl)-Phe-Ser-Lys-Gh-Tyr(N02)-Asp-NH2 (£Mapp « 22 \M; Incenta Limited) was used as the substrate. In both cases the substrate concentration employed in the assays was equivalent to the ^Mapp- The rate of conversion of substrate to product was derived from the slope of the increase in fluorescence monitored continuously over time.
Extracellular S. aureus V8 cysteine peptidase (staphylopairi) peptidase activity assays
S. aureus V8 was obtained from Prof. S. Arvidson, Karolinska Institute, Stockholm, Sweden. Extracellular S. aureus V8 cysteine peptidase (staphylopain) activity assays were carried out using partially purified S. aureus V8 culture supernatant (obtained from Dr. Peter Lambert, Aston University, Birrriingharn, U.K.). Activity assays were carried out in 10 mM BTP, pH 6.5 containing 1 mM EDTA, 5 mM 2-mercaptoethanol and ImM CaCl2 using two-times diluted partially purified extract. For the inhibition assays, Abz-Ile-Ala-Ala-Pro-Tyr(NO2)-Glu-NH2 (£MSPP ~ 117 uM; Incenta Limited) was used as the substrate at a concentration equivalent to KUPP. The rate of conversion of substrate to product was derived from the slope of the increase in fluorescence monitored continuously over time,
Foot-and-mouth disease leader peptidase (FMDV-LP) activity assays
Recombinant wild-type FMDV-LP was obtained from Dr. Tim Skern (Institut fur Medizinische Biochemie, Abteilung fur Biochemie, Universtat Wien, Wien, Austria). Activity assays were carried out in 50 mM trisacetate, pH 8.4 containing 1 mM EDTA, 10 mM L-cysteine and 0.25% (w/v) CHAPS using 10 nM enzyme. For the inhibition assays, Abz-Arg-Lys-Leu-Lys-Gly-Ala-Gly-Ser-Tyr(N02)-Glu-NH2 (&Mapp ~ 51 uM, Incenta Limited) was used as the substrate at a concentration equivalent to KMPP. The rate of conversion of substrate to product was derived from the slope of the increase in fluorescence monitored continuously over time.
Caspase peptidase activity assays
Caspases 1-10 were obtained from CN Biosciences or BioVision Inc. (Mountain View, CA, USA) and all assays were carried out in 50mM HEPES; pH 7.2, 10% (v/v) glycerol, 0.1% (w/v) CHAPS, 142 mM NaCl, 1 mM EDTA, 5 mM dithiothreitol (DTT) using 0.1-1 U per assay. For caspase 1, Ac-Leu-Glu-His-Asp-AMC was used as the substrate; for caspase 2, Z-Val-Asp-Val-Ala-Asp-AFC was used as the substrate; for caspase 3, Ac-Asp-Glu-Val-Asp-AMC was used as the
substrate; for caspase 4, Suc-Tyr-Val-Ala-Asp-AMC was used as the substrate; for caspase 5, Ac-Leu-Glu-His-Asp-AMC was used as the substrate; for caspase 6, Ac-Val-Glu-Ile-Asp-AMC was used as the substrate; for caspase 7, Ac-Asp-Glu-Val-Asp-AMC was used as the substrate; for caspase 8, Ac-Be-Glu-Thr-Asp-AMC was used as the substrate; for caspase 9, Ac-Leu-Glu-His-Asp-AMC was used as the substrate; for caspase 10, Ac-Ile-Glu-Thr-Asp-AMC was used as the substrate (Nicholson, D.W. and Thornberry, N.A., TIBS, 22, 299-306, 1997; Stermicke, H.R. and Salvesen, G.S., J. Biol Chem., 272(41), 25719-25723, 1997; Talanian, R.V., et. al., J. Biol. Chem., 272(15), 9677-9682, 1997; Wolf, B.B. and Green, D.R., J. Biol. Chem. , 274(29), 20049-20052, 1999). The rate of conversion of substrate to product was derived from the slope of the increase La fluorescence monitored continuously over time.
Measurement of the apparent macroscopic binding (Michaelis) constants CKMapp) for substrates
The apparent macroscopic binding constant (KMBPP) for each substrate was calculated, from the dependence of enzyme activity as a function of substrate concentration. The observed rates were plotted on the ordinate against the related substrate concentration on the abscissa and the data fitted by direct regression analysis (Prism v 3.02; GraphPad, San Diego, USA) using Equation 1 (Comish-Bowden, A. Fundamentals of enzyme kinetics Portland Press; 1995, 93-128.).
In Equation 1 'V is the observed initial rate, 'Fmaxapp' is the observed maximum activity at saturating substrate concentration, '£iviapp' is the apparent macroscopic binding (Michaelis) constant for the substrate, '[S0]' is the initial substrate concentration.
Measurement of the inhibition constants
The apparent inhibition constant (Kj) for each compound was determined on the basis that inhibition was reversible and occurred by a pure-competitive mechanism. The K\ values were calculated, from the dependence of enzyme activity as a function of inhibitor concentration, by direct regression analysis (Prism v 3.02) using Equation 2 (Cornish-Bowden, A., 1995.).

In Equation 2 'vi' is the observed residual activity, 'Fmaxap!" is the observed maximum activity (i.e. in the absence of inhibitor), '^Mapp> is the apparent macroscopic binding (Michaelis) constant for the substrate, e[S]' is the initial substrate concentration, 1K\ is the apparent dissociation constant and '[I]' is the inhibitor concentration.
In situations where the apparent dissociation constant (£iapp) approached the enzyme concentrations, the £iapp values were calculated using a quadratic solution in the form described by Equation 3 (Morrison, J.F. Trends Biochem. Sci.,_l, 102-105, 1982; Morrison, J.F. Biochim. Biophys. Act^ 185, 269-286, 1969; Stone, S.R. and Hofsteenge, J. Biochemistry, 25,4622-4628,1986).

In Equation 3 'vi' is the observed residual activity, 'F' is the difference between the maximum activity (i.e. in the absence of inhibitor) and minimum enzyme activity, CE0' is the total enzyme concentration, '^Tjapp' is the apparent dissociation constant and '!„' is the inhibitor concentration. Curves were fitted by non-linear regression
analysis (Prism) using a fixed value for the enzyme concentration. Equation 4 was used to account for the substrate kinetics, where 'Ki is the inhibition constant, '[S0]' is the initial substrate concentration and 'j5rMapp' is the apparent macroscopic binding (Michaelis) constant for the substrate (Morrison, 1982).
The second-order rate of reaction of inhibitor with enzyme
Where applicable, the concentration dependence of the observed rate of reaction (^obs) of each compound with enzyme was analysed by determining the rate of enzyme inactivation under pseudo-first order conditions in the presence of substrate (Morrison, J.F., TIBS, 102-105,1982; Tian, W.X. and Tsou, C.L., Biochemistry, 21, 1028-1032, 1982; Morrison, IF. and Walsh, C.T., from Meister (Ed.), Advances in Enzymol., 61, 201-301,1988; Tsou, C.L., from Meister (Ed.), Advances in Enzymol, 6\j 381-436,1988;). Assays were carried out by addition of various concentrations of inhibitor to assay buffer containing substrate. Assays were initiated by the addition of enzyme to the reaction mixture and the change in fluorescence monitored over time. During the course of the assay less than 10% of the substrate was consumed.
The activity fluorescence progress curves were fitted by non-linear regression analysis (Prism) using Eq. 5 (Morrison, 1969; Morrison, 1982); where T' is the fluorescence response, 't' is time, 'v0' is the initial velocity, 'vs' is the equilibrium steady-state velocity, 'A:0bs' is the observed pseudo first-order rate constant and 'D' is the intercept at time zero (i.e. the ordinate displacement of the curve). The second order rate constant was obtained from the slope of the line of a plot of &0bS versus the inhibitor concentration (i.e. &0bs/[I]). To correct for substrate kinetics, Eq. 6 was used, where '[S0]' is the iniitial substrate concentration and 'jK"Mapp' is the apparent macroscopic binding (Michaelis) constant for the substrate.
Compounds of the invention were tested by the above described assays and observed to exhibit cruzipain inhibitory activity or inhibitory activity against an alternative CA Cl cysteine protease with an in vitro Ki inhibitory constant of less than or equal to 100|aM. Exemplary inhibition data for a number of example compounds of the invention are given in table 2.
(TableRemove)

We claim:
1. A compound according to general formula (I) or general formula (II):

(Formula Removed)
wherein: R1 = C0-7-alkyl (when C = 0, R1 is simply hydrogen). C3-6-cycloaIkyl or Ar- C0-7-alkyl (when C = 0, R1 is simply an aromatic moiety Ar);
Z - O or S;
P1=CR5R6,
P2= CR7R8,
Q=CR9R10 or NR11 where R11 is chosen from C0-7-alkyl;
each of R2, R3. R5- R6, R7. R8. R9 and R10 is indcpendently chosen from C0-7-alkyl. C3-6-cycloaIkyl, Ar-C0-7-alkyl. O- C0-7,-alkyl. ()-C3.ft-cycloalkyl. O-Ar- C0-7-alkyl. S-C0-7-alkyl S-C3-6-cycloalkyl. S-Ar-C0-7-alkyl. NH- C0-7-alkyl. NH- C3-6-cycloaIkyl, NH-Ar-C0-7-alkyl, N(C0-7-alkyI)2, N( C3-6,.cycloalkyl)2 or N(Ar- C0-7-alkyl)::

Y = CRl2Rl3-CO, where R12, R13 are chosen from C0-7-alkyl, C3-6-cycloalkyl and Ar-C0-7-alkyl;
in the group (X)o, X =, CR,4RIS, where R14 and R15 are independently chosen from C0-7-alkyl, C3-6-cycloaIkyl and Ar-C0-7-alkyI and O is a number from zero to three;
in the group (W)n. W = O, S, C(O), S(O) or S(0)2 or NR16, where R16 is chosen from C0-7-alkyl, C3.6.cycloalkyl and Ar-C0-7-alkyl and n is zero or one;
in the group (V)m. V = C(O), C(S), S(O), S(O),. S«))2NH, OC(O), NHC(O), NHS(O), NHS(0)2, OC(0)NH, C(0)NH or CR17R18, where R17 and R18 are independently chosen from C0-7-alkyl, C3.6.cycloalkyl, Ar-C0-7-alkyl and m is a number from zero to three, provided that when m is greater than one, (V)m contains a maximum of one carbonyl or sulphonyl group;
U = a 5- to 7-membered monocyclic or a 8- to 1 l-mcmbered bicyclic ring which is selected from the group consisting of:
(Formula Removed)
wherein R ' is:
C0-7-alkyl, C3-6-cycIoalkyI, Ar-C0.7-aIkyl, O-C0-7-aIkyI, 0-C3-6-cycloalkyl, O-Ar-C0-7-alkyl, S-C0-7-aIkyl, S-C3-6-cycloalkyl, S-Ar-C0-7-aIkyl, NH-C0-7-alkyl, NH-C3-6-cycloalkyl, NH-Ar-C0-7-aIkyl, N(C0-7-alkyl)2, N(C3-6.cycloalkyI)2 or N(Ar-C0-7-alkyl)2; or, when part of a CHR19 or CR19 group, R19 may be halogen;
A is chosen from:
CH2, CHR19,0, S and NR20;
where R19 is as defined above; and R20 is chosen from:
C0-7-alkyl, C3-6-cycloalkyl and Ar-C0-7-alkyI;
B, D and G are independently chosen from:
CR19, where R19 is as defined above, or N or N-oxide (N→O);
E is chosen from:
CH22, CHR10, O, S and NR20, where R19 and R20 arc defined as above;
J, L, M, R, T. T2, T3 and T4 are independently chosen from:
CR19 and N or N-oxidc (N→0). where R19 is as defined above;
T.s is chosen from:
CH or N;
q is a number from one to three, thereby defining a 5-, 6- or 7-membered
ring;
and all salts, hydrates, solvates, complexes and prodrugs thereof.
2. A compound as claimed in claim 1 wherein independently or in combination:
a) Z is O,
b) P1 and P2 arc CH::
c) Q is CH2 or NH;
d) R1 is Co-7-alkyI or Ar- Co-7-alkyl;
e) Y is CR12R13CO where R12, R13 are selected from C0-7-alkyl, C3-6-cycloalkyl and Ar- C0-7-alkyl;
f) in the group (X)0, X is CR14R15 and each of R14 and R15 is selected from
C0-7-alkyl and Ar- C0-7-alkyl; g) in the group (W)n. W is O, S, S02, C(O) or NR16. where R16 is C0-4-
alkyl; and n is 0 or 1; h) in the group (V)m. V is C(O). C(0)NH or CR18, where R18 is C0-4-alkyl;
and m is 0 or 1; i) U is a 5- or 6- membered saturated or unsaturated heterocycle or a saturated or
unsaturated 9- or 10- membered heterocycle selected from the structures set out in
claim I.
3. A compound as claimed in claim 1 or claim 2 wherein R1 is hydrogen, C1-4alkyl or Ar-C1-4-alkyl.
4. A compound as claimed in anyone of claims 1 to 4 where (X)„- Y is selected from one of the following moieties:

(Formula Removed)
wherein R19, R2U and Ar are as defined above.
5. A compound as claimed in anyone of claims 1 to 4 wherein Y is CHRl3CO where R is
Ar-CH2-, where the aromatic ring is phenyl or monocyclic heterocycle, optionally
substituted with an R19 group, wherein R19 is as defined in claim 1; or R13 comprises
cyclohexylmethyl.
6. A compound as claimed in anyone of claims 1 to 5 wherein (X)0- Y is selected
from the following:

(Formula Removed)
wherein R19 and Ar are as defined previously

7. A compound as claimed in anyone of claims 1 lo 6, wherein (W)n-(X)0- Y is one of the following moieties:

(Formula Removed)
8. A compound as claimed in anyone of claims 1 to 7, wherein in the group (X)0: X is CR14R15, R14 is hydrogen, R15 is C1-4-alkyl; and o is 0 or 1.
9. A compound as claimed in anyone of claims 1 to 8 wherein, in the group (W)n:
W is 0, S, SO2 C(O) or NH where n is 0 or 1.
10. A compound as claimed in anyone of claims 1 to 9 wherein the group (W)n is: NH.

11. A compound as claimed in anyone of claims 1 to 16 wherein the combination U-(V)n (W)n-(X)0-Y or U-(V)m-(W)n-(X)0 is one of the following:
(Formula Removed)
12. A compound as claimed in anyone of claims 1 to 11 wherein the combination U-(V)n (W)n-(X)0-Y is one of the following:
(Formula Removed)
13. A compound as claimed in anyone of claims 1 to 12 wherein U comprises one of the following:
(Formula Removed)
wherein R19 is as defined previously.
14. A compound as claimed in anyone of claims 1 to 13. wherein U represents a group:
(Formula Removed)
wherein R19, D, E, G, J, L, M, R, T, T2, T3 and T4 are as defined previously.
15. A compound as claimed in anyone of claims 1 to 14, wherein U represents a group
(Formula Removed)
wherein R , D, E, G, J, L, M, R and T are as defined previously.
16. A compound as claimed in anyone of claims 1 to 15. wherein U represents a group

(Formula Removed)
wherein R19 .D. I. G. J and 1. are as defined previously.

17. A method of validating a known or putative cysteine protease as a therapeutic target,
the method comprising:
(a) assessing the in vitro binding of a compound as claimed in anyone of claims 1 to 16 to an isolated known or putative cysteine protease, providing a measure of'potency'; and optionally, one or more of the steps of:
(b) assessing the binding of a compound as claimed in anyone of claims 1 to 16 to closely related homologous proteases of the target and general house-keeping proteases (e.g. trypsin) to provides a measure of'selectivity';

(c) monitoring a cell-based functional marker of a particular cysteine protease activity, in the presence of a compound as claimed in anyone of claims 1 to 16; and
(d) monitoring an animal model-based functional marker of a particular cysteine protease activity, in the presence of a compound as claimed in anyone of claims 1 lol6.
18. The use of a compound as claimed in anyone of claims 1 to 16, or a pharmaceutically
or veterinarily acceptable salt thereof, in the validation of a known or putative cysteine
protease as a therapeutic target.
19. A compound as claimed in anyone of claims 1 to 16, or a pharmaceutically or \eterinarily acceptable salt thereof, for use in medicine.
20. Use of a compound as claimed in anyone of claims. 1 to 16, or a pharmaceutically or veterinarily acceptable salt thereof, in the preparation of a medicament for preventing or treating diseases in which the disease pathology may be modified by inhibiting a cysteine protease.
21. Use of a compound as claimed in anyone of claims 1 to 16, or a pharmaceutically or

veterinarily acceptable salt thereof, in the preparation of a medicament for treating infections caused by Pneumocystis carinii, Tryps'anoma cruzi, Trypsanoma brucei, Leishmania Mexicana, Clostridium histolyticum, Staphylococcus aureus, foot-and-mouth disease virus and Crithidia fusiculata; as well as in osteoporosis, autoimmunity, schistosomiasis, malaria, tumour metastasis, metachromatic leukodystrophy, muscular dystrophy and amytrophy.
22. Use of a compound as claimed in anyone of claims 1 to 16, or a pharmaceutical!}' or
veterinarily acceptable salt thereof, in the preparation of a medicament for preventing or
treating Chagas' disease.
23. Use of a compound as claimed in anyone of claims I to 16, or a pharmaceutical^ or veterinarily acceptable salt thereof, in the preparation of a medicament for treating an autoimmune disorder.
24. Use of a compound as claimed in anyone of claims 1 to 16, or a pharmaceutically or veterinarily acceptable salt thereof, in. the preparation of a medicament for treating osteoporosis.
25. A pharmaceutical or veterinary composition comprising one or mote compounds as claimed in anyone of claims 1 to 16 and a pharmaceutically or veterinarily acceptable carrier.
26. A process for the preparation of a pharmaceutical or veterinary composition as claimed in claim 25, the process comprising bringing the active compound(s) into association with the carrier, for example by admixture.

Documents:

01054-delnp-2003-abstract.pdf

01054-delnp-2003-claims.pdf

01054-delnp-2003-correspondence-others.pdf

01054-delnp-2003-description (complete)-09-09-2008.pdf

01054-delnp-2003-description (complete).pdf

01054-delnp-2003-form-1.pdf

01054-delnp-2003-form-18.pdf

01054-delnp-2003-form-2.pdf

01054-delnp-2003-form-3.pdf

01054-delnp-2003-form-5.pdf

01054-delnp-2003-gpa.pdf

01054-delnp-2003-pct-101.pdf

01054-delnp-2003-pct-210.pdf

01054-delnp-2003-pct-220.pdf

01054-delnp-2003-pct-306.pdf

01054-delnp-2003-pct-401.pdf

01054-delnp-2003-pct-409.pdf

01054-delnp-2003-pct-416.pdf

1054-DELNP-2003-Abstract-(09-09-2008).pdf

1054-DELNP-2003-Abstract-(12-09-2008).pdf

1054-DELNP-2003-Claims-(09-09-2008).pdf

1054-DELNP-2003-Claims-(12-09-2008).pdf

1054-DELNP-2003-Correspondence-Others-(09-09-2008).pdf

1054-DELNP-2003-Correspondence-Others-(11-09-2008).pdf

1054-DELNP-2003-Form-1-(12-09-2008).pdf

1054-DELNP-2003-Form-2-(12-09-2008).pdf

1054-DELNP-2003-GPA-(11-09-2008).pdf

1054-DELNP-2003-Others-Document-(09-09-2008).pdf

1054-DELNP-2003-Petition-137-(09-09-2008).pdf

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Patent Number

223705

Indian Patent Application Number

01054/DELNP/2003

PG Journal Number

40/2008

Publication Date

03-Oct-2008

Grant Date

19-Sep-2008

Date of Filing

07-Jul-2003

Name of Patentee

AMURA THERAPEUTICS LIMITED

Applicant Address

INCENTA HOUSE, HORIZON PARK, BARTON ROAD, CAMBRIDGE CB3 7AJ, UNITED KINGDOM.

Inventors:

#	Inventor's Name	Inventor's Address
1	QUIBELL MARTIN	AMURA THERAPEUTICS LIMITED INCENTA HOUSE, HORIZON PARK, BARTON ROAD, CAMBRIDGE CB3 7AJ, UNITED KINGDOM

PCT International Classification Number

C07D 491/04

PCT International Application Number

PCT/GB02/00184

PCT International Filing date

2002-01-17

PCT Conventions:

#	PCT Application Number	Date of Convention	Priority Country
1	0101179.0	2001-01-17	U.K.
2	60/275,359	2001-03-13	U.K.