Title of Invention

PROCESS FOR CONTROLLING SIALIC ACID CONTENT OF GLYCOPROTEINS PRODUCED IN MAMMALIAN CELL CULTURE

Abstract

The present invention relates to a process for controlling the amount of sialic acid present on an oligosaccharide ode chain of a glycoprotein produced by mammalian cell culture which comprises: .culturing the mammalian host cell in a production phase of the culture which is characterized by; i) adding an alkanoic acid or a salt thereof to the cell culture at a concentration of 0.1 mM to 20 mM; and ii) maintaining the osmolality of the cell culture at 250 to 600 mOsm.

Full Text

MM IMALIAM CELL CULTWIE PROOFS Field of the Invention This invention relates to processes for controlling the sialic acid content of glycoproteins produced in mammalian cell culture. The invention provides processes for increasing and decreasing the sialic acid content of glycoproteins produced by mammalian cell culture. The invention further relates to processes for producing tumor necrosis factor receptor (TNFR)-immunoglobulin (Ig) chimeras as well as novel TNFR1 -IgG, preparations and their uses m the diagnosis and treatment of various inflammatory and immune disorders. Description of Related Art Differences in glycosylation patterns of recombinantly produced glycoproteins have recently been the topic of much attention in the scientific community as recombinant proteins produced as probable prophylactics and therapeutics approach the clinic. The oligosaccharide side chains of the glycoproteins affect the protein's function (Wittwer A., and Howard. S.C. (1990) Biochem. 29:4175-4180) and the intramolecular interaction between portions of the glycoprotein resulting in the conformation and presented three dimensional surface of the glycoprotein (Hart, (1992) Curr. Op. Cell Biol., 4:1017-1023: Goochee, et al, (1991) Bio/Technology, 9:1347-1355; Parekh. R.B., (1991) Curr. Op. Struct. Biol., 1:750-754). Oligosaccharides may also serve to target a given polypeptide to certain structures based upon specific cellular carbohydrate receptors (Bevilacqua, M.P. and Nelson, R.M., (1993) I. Clin. Invest. 91:379-387; Nelson, R.M., etai, (1993) j. Clin. Invest. 91:1157-1166, Norgard. K.E. etai, (1993) Proc. Natl. Acad. Sci. USA 90:1068-1072; Imai, Y. etai, (1993) Nature 361:555-557). The terminal sialic acid component of the glycoprotein oligosaccharide side chain affects absorption, serum half life, and clearance from the serum, as well as the physical, chemical and immunogenic properties of the glycoprotein*Parekh, R.B.. supra; Varki, A., (1993) Glycobiology 3:97-100; Paulson. J. (1989), TIBS. 14:272-276; Goochee, et at., (1991) Biotechnology 9:1347-1355; Kobata, A. (1992) Eur. J. Biochem. 209:483-501). It is therefore important to maintain the sialic acid content of glycoproteins, particularly of those proteins intended for use as therapeutics. Much attention has been paid to the factors which affect glycosylation during recombinant protein production such as growth mode (adherent or suspension), fetal bovine serum in media formulation, culture density, oxygenation, pH, purification schemes and the like (Werner, R. and Noe, W. (1993), Drug Res. 43:1134-1249; Hayier et ai, (1992) Biotech, and Bioeng. 39:327-335; Borys et at., (1994) Biotech and Bioeng. 43:505-514; Borys et al, (1993) Bio/technology 11:720-724; Hearing etai, (1989) J. Cell Biol. 108:339-353; Goochee et al., in Frontiers in Bioprocessing II, Todd et at., eds (1992) American Chemical Society pp. 199-240; U.S. Patent No. 5,096,816; Chotigeat, W., (1994) Cytotech. 15:217-221). Several groups have investigated the process parameters that surround the production of recombinant proteins and especially the effect of media composition in the production of recombinant proteins (Park etai, (1992) Biotech. Bioeng. 40:686-696; Cox and McCIure, (1983) In Vitro, 19:1-6; Mizutani etai, (1992) Biochem. Biophys. Res. Comm. 187:664-669; Le Gros etai, (1985) Lymph. Res. 4(3):221-227). Addition of alkanoic acids such as butyric acid are known to effect the transient expression of foreign DNA in recombinant cell culture (Prasad, and Sinha, (1976) In Vitro, 12:125-132; Japanese Patent Application No. 62-48935; Japanese Patent Application No. 55-150440; Klehr et al, (1992) Biochem. 31:3222-3229; Gorman, and Howard, (1983) Nucleic acid Res. 11:7631-7648). However, sodium butyrate has a range of effects on gene expression across various cell lines and media compositions (D'Anna *ra(., (1980) Biochem. 19:2656- 1671; Hagopian, H.K., (1977] Cell 12:855-860) and protein production (Milhaud (1980) J. Cell. Physiol. 04:163-170, U.K. Patent Application No. GB 2 122 207 Al suggesting that butyrate may modify gene ixpression (Yuan et al., (I985| 1. Biol, Chem. 3778-3783) or inhibit (he expression of certain genes (Yuan et a!., supra). European Patent No. 0 239 292 B1 describes a process for the enhanced production of protein in the presence of an alkanoic acid or a salt thereof such as butyric acid. The publication, however, provides little guidance >n selecting appropriate concenlratjons of rhe additive and further does not address the effect of (he additive on protein glycosylation. Others have described that the addition of low levels (0-1.5mM) of sodium butyrate to cell culture production medium to increase cell specific productivity leads to concomitant increases in acidic glycoforms (corresponding to increased sialic acid content) of the recombinant protein produced (Chotigeai, etal, (1994) Cytotecb. 15:2:17-221). Several groups have looked at the effects of osmolality on cell growth and polypeptide production (Ozturk and Palsson (1991) Biotech, and Bioeng. 37:989-993; Stubblefield et al. (1960) Cancer Research, 20:1646-1655; Garcia-Perezef a/-, Others have discussed the effect of glucose concentration on cell growth and/or polypeptide production m recombinant cell culture. See, for example, Parker al., (1992) Biotechnology and Bioengineering, 40:686-696; Huang etal, (1991) Journal of Biotechnology, 18:161-162; EP 387,840; Reuveny etal, (1986) Journal of Immunological Methods, 86:53-59; Fine etal, (1976) In Vitro, I2(10):693-701; Dircks etal, (1987) Exp. ■ Eye Res., 44:951-958; Mizutani et al, (1992) Biochemical and Biophysical Research Communications, 187(2):664-669; Sugiura, (1992) Biotechnology and Bioengineering, 39:953-959; WO 88/01643; Graf et al, (1989) DECHEMA Biotechnol. Conf., 3:615-618; Japanese Patent Application No. JP 1-101882; U.S. Patent No. 3,926,723; WO 87/00195; and Reischaker, Jr., Ph.D. Thesis, Massachusetts Institute of Technology, pp. 196-229 (June 1982). However, the previous studies have not studied the effect of various process parameters on the sialic acid content of the mature protein, a factor in glycoprotein production that is tantamount to clinical success. The present invention provides for processes for.controlling the content of sialic acid of glycoproteins produced by mammalian cell culture. Summary of the Invention The present inventors have discovered that certain mammalian cell culture process parameters affect cell specific productivity as well as the extent and type of glycosylation of the proteins produced. More particularly, the present inventors have found that certain factors which enhance cell specific productivity have an inverse effect on the sialic acid content of the produced protein. The present inventors have therefore devised various cell culture processes to enncb particular gJvcoforms of glycoproteins produced in mammalian cell culture. Accordingly, the invention provides for a process for controlling (he sialic acid content of a glycoprotein produced by mammalian cell culture. According to this aspect of the invention, varying the production rate of the glycoprotein in the production phase of the cell culture leads to variations in the sialic acid content of the mature glycoprotein. More particularly, an increase in cell specific productivity during the glycoprotein production phase results in a decrease in sialic acid content of the mature protein. Conversely, a decrease in cell specific productivity results in an increase in sialic acid content in the mature protein., - The present invention provides, in a particular embodiment, for varying the cell specific productivity of a mammalian host cell during the protein production phase of mammalian cell culture by controlling factors which affect cell specific productivity. According to one aspect of the invention, the concentration of factors which enhance DNA transcription are controlled. In another embodiment cell specific productivity is controlled by maintaining the osmolality of the cell culture within certain margins. According to the invention, any of the foregoing parameters are controlled, alone or in combination, to affect the mature glycoprotein sialic acid content. In a particular embodiment of the present invention, the factor which enhances DNA transcription is an alkanoic acid or salt thereof such as sodium butyrate at a concentration of about 0.1 mM to about 20 mM. According to a second aspect of the invention, the osmolality of the cell culture is maintained between about 250-600 mOsm. In a further aspect, the temperature of the cell culture is controlled between about 30°C and 37"C. [n a preferred embodiment, the invention provides for a process for increasing the sialic acid content of the mature glycoprotein produced by mammalian cell culture comprising maintaining a lower cell specific productivity by controlling any or all of the above identified process parameters, optionally together with other parameters known in the art. According to this aspect of the present invention, culturing the host cell at a concentration of the alkanoic acid or salt thereof of about 0.1 mM to about 6mM, and optionally together with maintaining the osmolality of the cell culture at about 300-450 mOsm produces a protein with an increased sialic acid content. In a further preferred embodiment, the invention provides for a process for decreasing the sialic acid content of the mature glycoprotein produced by mammalian cell culture comprising increasing cell specific productivity of the cell culture. The cell specific productivity is increased, in a preferred embodiment, by providing a cell culture process which comprises any of, culturing the host cell at a concentration of an alkanoic acid or salt thereof of about 6 mM to about 12mM; and, maintaining the osmolality of the cell culture at about 450-600 mOsm. The invention further provides, in a particular embodiment, for a cell culture process with three phases of cell cullure. The invention therefore provides a process for controlling the sialic acid content of a glycoprotein produced by mammalian cell culture comprising the steps of culturing a host cell which expresses the protein in a growth phase for a period of time and under such conditions that cell growth is maximized. According to this aspect of (he present invention, the growth phase is followed by a transition phase in which cell culture parameters for the desired sialic acid content of the mature glycoprotein are selected and engaged. The transition phase is followed by a production phase of the cell culture wherein parameters selected in the transition phase are maintained and glycoprotein product is produced and harvested. Varying the cell specific productivity of -> ihe production phase of the cell culture by adding an alkanoic acid or a salt thereof to the cell culture at a concentration of about 0.1 mM to about 20 mM and engaging an osmolality of the cell culture at ahout between 250 and 600 mOsm. optionally in combination with one another during the transition phase produces a protein with differing amounts of sialic acid. In a further preferred embodiment, the present invention provides a process for controlling the amount of sialic acid present in a soluble type 1 tumor necrosis factor receptor (TNFRl)-immunoglobulin G,(IgG|) chimeric protein. The present inventors have discovered that, under certain conditions of production, novel TNFRl-IgG, glycoform preparations may be obtained which exhibit the desirable properties of prolonged clearance from the blood while retaining significant functional activity. A long functional half-life permits simplified, bolus-dose administration and contributes to in vivo potency of the glycoprotein produced allowing for lower dose forms of the glycoprotein. According to this aspect of the present invention, a TNFRl-IgG, glycoprotein molecule is produced that contains increased sialic acid residues. The cell culture parameters for the production phase of the TNFRl-IgG, are selected to obtain the desired sialic acid content. In a preferred embodiment, the sodium butyrate is present in the production phase at a concentration of about 0.1 to about 6 mM and the osmolality is maintained at about 300-450 mOsm. In a more preferred aspect the sodium butyrate concentration is about 1 mM and the osmolality is maintained at about 350-400 mOsm, In yet another embodiment, the present invention provides for a preparation of TNFRl-IgG, glycoprotein produced by the process of the present invention. According to this aspect of the invention a preparation is provided comprising TNFRl-IgG, in which the range of pi of the preparation is between about 5.5 and 7.5. Further provided is a TNFRl-IgG, preparation having a molar ratio of sialic acid to protein of about 4 to about 7 and especially about 5 to about 6. In yet another aspect, the TNFRI-IgGl preparation has about 1 to about 2 moles of exposed N-acerylglucosamine residues per mole of protein. In a further aspect, the preparation has a molar ratio of sialic acid to N-acetylglucosamine of about 0.35 to about 0.5 and more preferably about 0.4 to about 0.45. The present invention also provides a therapeutic composition comprising the above preparation useful in the treatment of TNF-mediated pathologic conditions. Description of the Drawings Figure 1A and Figure IB. Figures lAand IB show the method used lo calculate specific productivity of a typical cell culture process in die production phase. The specific productivity can be expressed as a function of the viable cell counts (viable cell days) as shown in Figure 1A; or packed cell volume (PCV) as shown in Figure IB. The specific production rate is given with the range for a 90% confidence interval. Figure 2A and Figure 2B. Figures 2A and 2B show the correlation between specific productivity based on viable cell days (Figure 2A) and packed cell volume (PCVXFigure 2B) during the production phase and the sialic acid (NANA) content of the harvested product. Values for 9 different processes (A-I) are shown. The data points represent independent production processes as described in Table I. Detailed Description of the Invention I. Definitions The carbohydrate moieties of the present invention tvill be described with reference to commonly used nomenclature for the description of oligosaccharides. A review of carbohydrate chemistry which uses this nomenclature is found in Hubbard and Ivatt (1981) Ann., Rev. Biochem. 50:555-583. This nomenclature includes, for instance, Man, which represents mannose; GlcNAc, which represents 2-N-acetylglucosamine, Gal which represents galactose; and Glc, which represents glucose. Sialic acids are described with reference to the shorthand notation NeuNAc, for 5-N-acety(neuraminic acid, and NeuNGc for 5-gIycoly[neuraminic acid (J. Biol. Chem. 1982 257:3347; J. Biol. Chem., 1982. 257:3352). "Osmolality" is a measure of the osmotic pressure of dissolved solute particles in an aqueous solution. The solute particles include bodi ions and non-ionized molecules. Osmolality is expressed as the concentration of osmotically active particles (i.e., osmoles) dissolved in 1 kg of solution (1 mOsm/kg H;0 at 38°C is equivalent to an osmotic pressure of 19mm Hg). "Osmolality," by contrast, refers to the number of solute particles dissolved in 1 liter of solution. When used herein, the abbreviation "mOsm" means "milliosmoles/kg solution". As used herein, "glycoprotein" refers generally to peptides and proteins having more than about ten amino acids and at least one oligosaccharide side chain. The glycoproteins may be homologous to the host cell, or preferably, they are heterologous, i.e.. foreign, to the host cell being utilized, such as a human protein produced by a Chinese hamster ovary cell. Preferably, mammalian glycoproteins (glycoproteins that were originally derived from a mammalian organism) are used, more preferably, those which are directly secreted into ihe medium. Examples of mammalian glycoproteins include molecules such as cytokines and iheir receptors, as well as chimeric proteins comprising cytokines or their receptors, including, for instance tumor necrosis factor alpha and beta, their receptors (TNFR-1; EP 417,563 published 20 March 1991; and TNFR-2. EP 417,014 published 20 March 1991) and their derivatives; renin; a growth hormone, including human growth hormone, and bovine growth hormone; growth hormone releasing factor; parathyroid hormone; thyroid stimulating hormone; lipoproteins; alpha-l-amitrypsin; insulin A-chain; insulin B-chain; proinsulirt; follicle stimulating hormone; calcitonin; luteinizing hormone; glucagon; clotting factors such as factor VIIIC, factor IX. tissue factor", and von Willebrands factor; anti-clotting factors such as Protein C; atrial natriuretic factor; lung surfactant: a plasminogen activator, such as urokinase or human urine or tissue-type plasminogen activator (t-PA); bombesin; thrombin; hemopoietic growth factor; enkephalinase; RANTES (regulated on activation normally T-cell expressed and secreted); human macrophage inflammatory protein (MIP-I-aIpha); a serum albumin such as human serum albumin; mullerian-inhibiting substance; relaxin A-chain; relaxin B-chain; prorelaxin; mouse gonadotropin-associated peptide; a microbial protein, such as beta-lactamase; DNase; inhibin; activin; vascular endothelial growth factor (VEGF); receptors for hormones or growth factors; integrin; protein A or D; rheumatoid factors; a neurotrophic factor such as bone-derived neurotrophic factor (BDNF), neurotrophin-3, -4, -5, or -6 (NT-3,NT-4, NT-5, or NT-6), or a nerve growth factor such as NGF-p; platelet-derived growth factor (PDGF); fibroblast growth factor such as aFGF and bFGF; epidermal growth factor {EGF); transforming growth factor (TGF) such as TGF-alpha and TGF-beta, including TGF-p 1, TGF-(J2, TGF-03, TGF-P4, or TGF-P5; insulin-like growth faclor-I and -II (IGF-I and IGF-II); des( 1-3HGF-I (brain IGF-I), insulin-like growth factor binding proteins; CD proteins such as CD-3, CD-4, CD-8, and CD-19; erythropoietin; osteoinductive factors; immunotoxins; a bone morphogenetic protein (BMP); an interferon such as interferon-alpha, -beta, and -gamma; colony stimulating factors (CSFs), e.g., M-CSF. GM-CSF, and G-CSF; interleukins (ILs), e.g., IL-I to IL-10; superoxide dismutase; T-cell receptors; surface membrane proteins; decay accelerating factor; viral antigen such as, for example, a portion of the AIDS envelope; transport proteins; homing receptors; addressins; regulatory proteins; antibodies; chimeric proteins, such as immunoadhesins, and fragments of any of the above-listed polypeptides. The terms "cell culture medium" and "culture medium" refer to a nutrient solution used for growing mammalian cells that typically provides at least one component from one or more of the following categories; 11 an energy source, usually in the form of a carbohydrate such as glucose; 2) all essential amino acids, and usually the basic set of twenty amino acids plus cysteine; 3) vitamins and/or other organic compounds required at low concentrations; 4) free fatty acids; and 5) trace elements, where trace elements are defined as inorganic compounds or naturally occurring elements that are typically required at very low concentrations, usually in the micro molar range. The nutrient solution may optionally be supplemented with one or more components from any of the following categories; 1) hormones and other growth factors as, for example, insulin, transferrin, and epidermal growth factor; 2) salts and buffers as, for example, calcium, magnesium, and phosphate; 3} nucleosides and bases such as, for example, adenosine, thymidine, and hypoxanthine; and 4} protein and tissue hydrolysates. The term "mammalian host cell", "host cell", "mammalian cell" and the like, refer to cell lines derived from mammals that are capable of growth and survival when placed in either monolayer culture or in suspension culture in a medium containing the appropriate nutrients and growth factors. The necessary growth factors for a particular cell line are readily determined empirically without undue experimentation, as described for example in Mammalian Cell Culture (Mather, J.P. ed„ Plenum Press, N.Y. [1984]), and Barnes and Sato, (1980) Cell, 22:649. Typically, the cells are capable of expressing and secreting large quantities of a particular glycoprotein of interest into the culture medium. Examples of suitable mammalian host cells within the context of the present invention may include Chinese hamster ovary cells/-DHFR (CHO, Urlaub and Chasin, Proc. Natl. Acad. Sci. USA, 77:4216 [1980]); dpl2.CHO cells {EP 307,247 published 15 March 1989); monkey kidney CVI line transformed by SV40 (COS-7, ATCC CRL 1651); human embryonic kidney line (293 or 293 cells subcloned for growth in suspension culture, Graham etai, J. Gen Virol., 36:59 [1977]); baby hamster kidney cells (BHK, ATCC CCL 10); mouse senoli cells (TM4, Mather. Biol. Reprod., 23:243-251 [1980]); monkey kidney cells (CVI ATCC CCL 70); African green monkey kidney celts (VERO-76. ATCC CRL-1587); human cervical carcinoma cells (HELA, ATCC CCL 2); canine kidney cells (MDCK, ATCC CCL 34); buffalo rat liver cells (BRL 3A. ATCC CRL 1442); human lung cells (W138. ATCC CCL 75); human liver cells (Hep G2, HB 8065); mouse mammary tumor (MMT 060562, ATCC CCL51); TRI cells (Mather et ai. Annals N.Y. Acad. Sci.. 383:44-68 [ 1982]); MRC 5 cells; FS4 cells; and a human hepatoma line (Hep G2). Preferred host cells include Chinese hamster ovary cells/-DHFR (CHO, Urlaub and Chasin. Proc. Natl, Acad. Sci. USA. 77:4216 [1980]); dpl2.CHO cells (EP 307,247 published 15 March 1989). The term "peplone" within the context of the present invention is meant to refer to a media supplement that is essentially hydrolyzed animal protein. The source of this protein may be animal by-products from slaughter houses, purified gelatin, or plant material. The protein is typically hydrolyzed using acid, heat or various enzyme preparations. Preferred peptone mixtures are, for example. "Pnmatone RL" and "Primatone HS". boih of which are commercially available (Sheffield, England). "Cell specific productivity", "cell specific rate" and ihe like, are used herein to refer to the specific, i.e., per cell, or per measure of cell mass or volume, product expression rate. The cell specific productivity is measured in, for example, grams of protein produced per cell per day. Cell specific productivity can be conveniently measured according to the integral method: dP/dt =qp X, or P = qp/:: Xdt where qp is the cell specific productivity constant, X is the number of cells or cell volume, or cell mass equivalents, and dP/dt is the rate of protein production. Hence, qp can be obtained from a plot of product concentration versus time integral of viable cells ( }.c Xdt "viable cell days"). According to this formula, when the amount of glycoprotein product produced is plotted against the viable cell days the slope is equivalent to the ceil specific rate. Viable cells can be determined by several measures, for example, biomass, 0, uptake rate, lactase dehydrogenase (LDH), packed cell volume or turbidity. "Growth phase" of the cell culture refers to the period of exponential cell growth {the log phase) where cells are generally rapidly dividing. During this phase, cells are cultured for a period of time, usually between 1 -4 days, and under such conditions that cell growth is maximized. The determination of the growth cycle for the host cell can be determined for the panicutar host cell envisioned without undue experimentation. "Period of time and under such conditions that cell growth is maximized" and the like, refer to those culture conditions that, for a particular cell line, are determined to be optimal for cell growth and division. During the growth phase, cells are cultured in nutrient medium containing the necessary additives generally al about 30-40° C, in a humidified, controlled atmosphere, such that optimal growth is achieved for the particular cell line. Cells are maintained in the growth phase for a period of about between one and four days, usually between two to three days. "Transition phase" of the cell culture refers to the period of time during which culture conditions for the production phase are engaged. During the transition phase environmental factors such as temperature of the cell culture, medium osmolality and the like are shifted from growth conditions to production conditions. "Production phase" of the cell culture refers to (he period of time during which cell growth has plateaued. During the production phase, logarithmic cell growth has ended and protein production is primary. During this period of time the medium is generally supplemented to support continued protein production and to achieve the desired glycoprotein product. The term "expression" or "expresses" are used herein to refer to transcription and translation occurring within a host cell. The level of expression of a product gene in a host cell may be determined on the basis of either the amount of corresponding mRNA that is present in the cell or the amount of the protein encoded by the product gene that is produced by the cell. For example. mRNA transcribed from a product gene is desirably quantitated by northern hybridization. Sambrook«u/.. Molecular Cloning: A Laboratory Manual, pp. 7.3-7.57 (Cold Spring Harbor Laboratory Press, 1989). Protein encoded by a product gene can be quantitated either by assaying for the biological activity of the protein or by employing assays that are independent of such activity, such as western blotting or radioimmunoassay using antibodies that are capable of reacting with the protein. Sambrook ti ai. Molecular Cloning: A Laboratory Manual, pp. 18.1-18.88 (Cold Spring Harbor Laboratory Press, 1989). By alkanoic acid or salt thereof is meant a straight or branched chain, saturated or unsaturated, alkanoic acid or salt thereof. The alkanoic acid is generally from one to ten carbon atoms and is preferably from three to six carbon atoms. An exemplary alkanoic acid is butyric acid and the corresponding salt is sodium butyrate. II. Cell Culture Procedures The present inventors have discovered that factors which increase cell specific productivity during the production of a glycoprotein produced by mammalian cell culture have an inverse effect on sialic acid content of the glycoprotein produced. Since proteins expressing one or more sialic acid residues per complex oligosaccharide structure have longer clearance rates in vivo the clearance rate of the glycoprotein produced may be manipulated within broad limits by the overall degree of sialylation of the preparation. The present invention provides for processes for controlling the extent of sialylation of a glycoprotein produced by mammaiian cell culture. Following the methodology set forth herein, one is able to determine the precise process parameters that provide for the desired sialic acid content of a glycoprotein produced by mammalian ceil culture. According to the present invention a mammalian host cell is cultured to produce a recoverable glycoprotein product. The overall content of sialic acid in the glycoprotein is controlled by controlling cell culture parameters which affect cell specific productivity. Factors which affect cell specific productivity are well known in the art and include, but are not limited to, factors which affect DNA/RNA copy number, factors which affect RNA, such as factors which stabilize RNA, media nutrients and other supplements, the concentration of transcription enhancers, the osmolality of the culture environment, the temperature and pH of the cell culture, ..nd the like. According to the present invention adjustment of these factors, alone or in combination, to increase cell specific productivity generates a protein with a decreased sialic acid content. The adjustment of these factors, alone or in combination, to decrease cell specific productivity, generates a mature glycoprotein with an increased sialic acid content. The invention will now be described with reference to various, including well known, cell culture techniques and principles. According to the present invention mammalian cells are cultured to produce a desired glycoprotein product. In choosing a host cell for the production of the glycoprotein within the context of the present invention, it is important to recognize that different host cells have characteristic and specific mechanisms for the translational and post-translational processing and modification (e.g., glycosylation, cleavage) of the proteins expressed. Appropriate cell lines should be chosen to ensure that the desired post translational modifications are possible. Alternatively, host cells can be modified to express a desired gene product required for the specific post-translational modification. In particular, ihe mammalian cells which express the desired protein should express or be manipulated to express the particular enzymes such that under the appropriate conditions, described herein, the appropriate post translational modification occurs in vivo. The enzymes include those enzymes necessary for the addition and completion of N- and O- linked carbohydrates such as those described in Hubbard and Zvan supra for N-Iinked oligosaccharides. The enzymes optionally include oligosaccharyltransferase, alpha-glucosidase I. alpha-glucosidase II, ER alpha(l,2)mannosidase, Golgi alpha-mannodase I, N-acetylyglucosaminyltransferase I. Golgi alpha-mannodase II, N-acetylyglucosaminy I transferase II, alpha(l,6>fucosyltransferase, and S(1.4)galactosyltranferase. Additionally, the host cell expresses the appropriate sialyl transferase that can be expected to attach the terminal sialic acid in specific position and linkage as part of the host cell genome. Optionally, ihe host cell can be made to express the appropriate sialyl transferases by, for instance, transfection of the host cell with the DNA encoding the sialyl tranferase. The sialyl transferases described above would be expected to add the terminal sialic acid residue to the appropriate oligosaccharide core structure such as GalBl-4GlcNAc. Appropriate sialyl transferases within the context of the present invention include, but are not limited to, those sialyl transferases which catalyze the complex sialylation and branching of the N- and O-linked oligosaccharides. For the culture of the mammalian cells expressing the desired protein and capable of adding the desired carbohydrates in specific position and linkage, numerous culture conditions can be used paying particular attention to the host cell being cultured. Suitable culture conditions for mammalian cells are well known in the an (J. Immunol. Methods (1983)56:221-234) or can be easily determined by me skilled artisan (see. for example. Animal Cell Culture: A Practical Approach 2nd Ed., Rickwood. D. and Hames, B.D., eds. Oxford University Press. New York (1992)), and vary according to the particular host cell selected. The mammalian ceil culture of the present invention is prepared in a medium suitable for the particular cell being cultured. Commercially available media such as Ham's F10 (Sigma), Minimal Essential Medium ([MEM], Sigma), RPMI-I640 (Sigma), and Dulbecco's Modified Eagle's Medium ([DMEM], Sigma) are exemplary nutrient solutions. In addition, any of the media described in Ham and Wallace,(1979) Meth. Enz., 58:44; Barnes and Sato,(1980) Anal. Biochem., 102:255; U.S. Patent Nos. 4,767.704; 4.657.866; 4,927.762; 5.122,469 or 4,560.655; International Publication Nos. WO 90/03430; and WO 87/00195; the disclosures of all of which are incorporated herein by reference, may be used as culture media. Any of these media may be supplemented as necessary with hormones and/or other growth factors (such as insulin, transferrin, or epidermal growth factor), salts (such as sodium chloride, calcium, magnesium, and phosphate), buffers (such as HEPES), nucleosides (such as adenosine and thymidine), antibiotics (such as Gentamycin™ drug), trace elements (defined as inorganic compounds usually present at final concentrations in the micromolar range) lipids (such as linoleic or other fatty acids) and their suitable carriers, and glucose or an equivalent energy source. Any other necessary supplements may also be included at appropriate concentrations that would be known to those skilled in the art. In a particular embodiment, the mammalian host cell is a CHO cell, preferably a dpl2.CHO cell and a suitable medium contains a basal medium component such as a DMEM/HAM F-12 based formulation (for composition of DMEM and HAM F12 media, see culture media formulations in American Type Culture Collection Catalogue of Cell Lines and Hybridomas, Sixth Edition, 1988, pages 346-349) (the formulation of medium as described in U.S. Patent 5.122,469 are particularly appropriate) with modified concentrations of some components such as amino acids, salts, sugar, and vitamins, and optionally containing glycine, hypoxanthine, and thymidine; recombinant human insulin, hydrolyzed peptone, such as Primatone HS or Pnmatone RL (Sheffield. England), or the equivalent; a cell protective agent, such as Pluromc F68 or the equivalent pluronic polyol; Gentamycin; and trace elements. The glycoproteins of the present invention may be produced by growing cells which express the desired protein under a variety of cell culture conditions. For instance, cell culture procedures for the large or small scale production of proteins are potentially useful within the context of the present invention. Procedures including, but not limited to, a fluidized bed bioreactor, hollow fiber bioreactor, roller bottle culture, or stirred tank bioreactor system may be used, in the later two systems, with or without microcarriers, and operated alternatively in a batch, fed-batch, or continuous mode. In a preferred embodiment the cell culture of the present invention is performed in a stirred tank bioreactor system and a fed batch culture procedure is employed. In the preferred fed batch culture the mammalian host cells and culture medium are supplied to a culturing vessel initially and additional culture nutrients are fed, continuously or in discrete increments, to the culture during culturing, with or without periodic cell and/or product harvest before termination of culture. The fed batch culture can include, for example, a semi-continuous fed batch culture, wherein periodically whole culture (including cells and medium) is removed and replaced by fresh medium. Fed batch culture is distinguished from simple batch culture in which all components for cell culturing (including the cells and all culture nutrients) are supplied to the culturing vessel at the start of the culturing process. Fed batch culture can be further distinguished from perfusion culturing insofar as the supemate is not removed from the culturing vessel during the process (in perfusion culturing, the cells are restrained in the culture by, e.g.. tiltraiion, encapsulation, anchoring to microcarriers etc. and the culture medium is continuously or intermittently introduced and removed from the culturing vessel). Further, the cells of the culture may be propagated according to any scheme or routine that may be suitable for the particular host cell and the particular production plan contemplated. Therefore, the present invention contemplates a single step or multiple step culture procedure. In a single step culture the host cells are inoculated into a culture environment and the processes of the instant invention are employed during a single production phase of the cell culture. Alternatively, a mulu-stage culture is envisioned. In the multi-stage culture cells may be cultivated in a number of steps or phases. For instance, cells may be grown in a first step or growth phase culture wherein cells, possibly removed from storage, are inoculated into a medium suitable for promoting growth and high viability. The cells may be maintained in the growth phase for a suitable period of time by the addition of fresh medium to the host cell culture. According to a preferred aspect of the invention, fed batch or continuous cell culture conditions are devised to enhance growth of the mammalian cells in the growth phase of the cell culture. In the growth phase cells are grown under conditions and for a period of time that is maximized for growth. Culture conditions, such as temperature, pH, dissolved oxygen (dO:) and the like, are diose used with the particular host and will be apparent to the ordinarily skilled artisan. Generally, the pH is adjusted to a level between about 6.5 and 7.5 using either an acid (e.g., CO.) or a base (e.g., Na,CO, or NaOH). A suitable temperature range for culturing mammalian cells such as CHO cells is between about 30 to 38°C and a suitable dO, is between 5-90% of air saturation. Al a particular stage the cells may be used to inoculate a production phase or step of the cell culture. Alternatively, as described above the production phase or step may be continuous with the inoculation or growth phase or step. According to the present invention, the cell culture environment during the production phase of ihe cell culture is controlled. According to the process of the present invention, factors affecting cell specific productivity of the mammalian host cell are manipulated such that the desired sialic acid content is achieved in the resulting glycoprotein. In particular, factors which increase cell specific productivity are controlled during the production phase of the cull culture process such that the resulting glycoprotein product contains the desired sialic acid content. In a preferred aspect, the production phase of the cell culture process is preceded by a transition phase of the cell culture in which parameters for the production phase of the cell culture are engaged. According to one aspect of die present invention the concentration of a transcription enhancer such as an alkanoic acid, is manipulated to affect cell specific productivity and merefore the resulting sialic acid content of the mammalian cell glycoprotein product. The alkanoic acid may be any one of a number of single or branched chain alkanoic acids that enhance transcription of mammalian proteins. In a preferred embodiment the alkanoic acid is butyric acid and especially the salt thereof, sodium butyrate. According to the present invention the concentration of sodium butyrate is controlled to control the cell specific productivity. Concentrations of sodium butyrate of between 0.1 and 20 mM are used and modified according to the particular host cell being cultured and the desired sialic acid content of the glycoprotein produced. In order to generate a protein with the desired sialic acid content a concentration of sodium butyrate is chosen which provides for the highest cell specific productivity with the most acceptable sialic acid profile. Therefore, according to die present invention concentrations of a transcription enhancer such as sodium butyrate are chosen to obtain the desired sialic acid content. To increase sialic acid content of the mature glycoprotein, generally, lower concentrations of the transcription enhancer are used. The lower concentration provides for enhanced transcription, but maintains lower cell specific productivity while maintaining the viability of the host cell culture. Generally concentrations of the transcription enhancer such as sodium butyrate between about 0.1 mM and about 8 mM are used. More preferably concentrations between about 1.0 and 6.0 mM are used. In a particular embodiment about 6 mM sodium butyrate is used. In another embodiment about ImM sodium butyrate is used. In another embodiment a glycoprotein is produced with a decreased level of sialic acid. According to this aspect of the present invention, mammalian cells are cultured under conditions such that cell specific productivity is increased. According to this aspect of the present invention a concentration of alkanoic acid or other appropriate transcription enhancer is chosen such that increased cell specific productivity generates a protein with the desired sialic acid profile. In a preferred embodiment the concentration of the alkanoic acid or salt thereof is between about 5 and 20 mM and more preferably between about 6 mM and 12 mM. In a panicular embodiment, the concentration of sodium butyrate is about 12 mM. In determining the appropriate concentration of the transcription enhancer such as an alkanoic acid or salt thereof, reference can be made to Figure 2 as well as Table I infra at Example I. According to the present invention lower butyrate concentrations generally result in lower cell specific productivity. According to the invention concentrations of sodium butyrate are chosen keeping in mind other process parameters such as the osmolality of the production phase. As discussed below the osmolality can affect the cell specific productivity. Concentrations of butyrate are chosen keeping in mind the particular osmolality to be maintained during the production phase. Alternatively, for other mammalian host cells and other glycoproteins, small test cultures can be prepared and rate of glycoprotein product production, i.e. the cell specific productivity can be determined and the resulting sialic acid content can be used to prepare a similar tabic and figure appropriate for (he particular host cell being cultured, keeping in mind that decreases in cell specific productivity lead to increases in the sialic acid content of the glycoprotein produced. The alkanoic acid or salt thereof, such as sodium butyrate, or other appropriate transcription enhancer is added to the host cell culture at or about the time the production phase of the cell culture is initiated. Conveniently, a transition phase is employed during the cell culture process preceding the production phase in which the cell culture conditions as discussed herein are engaged for the desired ceil specific productivity and hence the desired glycofbrm profile. The alkanoic acid or salt thereof is added by any means known in the art. In a preferred embodiment the sodium butyrate is added in batch to the fed batch culture system with or without other appropriate nutrients as described herein or known to those skilled in the art of mammalian cell culture. According to the instant invention the osmolality of the cell culture environment is controlled in addition to the factors noted above to regulate the extent of sialylation of the mature glycoprotein. In one embodiment, osmolality is controlled to control the sialic acid content of the mature protein independent of" other factors which affect cell specific productivity. In another embodiment osmolality is controlled in addition to controlling other factors which affect cell specific productivity. In a preferred embodiment both osmolality and the concentration of alkanoic acid are controlled. The osmolality of the cell culture environment is controlled to produce the desired balance between the cell specific productivity and the sialic acid profile of the resulting glycoprotein. Generally, cell specific productivity is increased when osmolality is increased. An osmolality which produces a protein with the desired sialic acid is chosen keeping in mind that increases in the osmolality generally lead to increase in production rate of the particular protein. In order to decrease the production rate and increase the sialic acid content of the. mature glycoprotein osmolality is generally maintained at a lower level for the particular cell type being cultured. For mammalian cell culture the osmolality of me culture medium is generally about 290-330 mOsm. However, increases in the osmolality generally lead to increase in production rate of proteins. An osmolality is chosen such mat the production rate corresponds with the desired product profile. To increase the sialic acid content, production rate is decreased and osmolality is generally maintained within a lower margin keeping in mind the particular host cell being cultured. Osmolality in the range from about 250 mOsm to about 450 mOsm is appropriate for an increased sialic acid content. More preferably, the osmolality is maintained at about between 300 and 450 mOsm, according to this aspect of the invention, and more preferably between about 350 and 400 mOsm. and most preferably about 400 mOsm. For a lower sialic acid conient, an osmolality which provides an increased production rate is chosen. According to this aspect of the invention, the osmolality is maintained at about between 350 - 600 mOsm. and is preferably between about 450-550 mOsm. according to this aspect of the invention. The skilled practitioner will recognize that media osmolality is dependent upon the concentration of osmotically active panicles in the culture fluid and thai a number of variables that make up a complex mammalian cell culture medium impact osmolality. The initial osmolality of the culture medium is determined by the composition of the culture medium. The osmolality can be measured using an osmometer such as that sold by Fisher Scientific, Pittsburgh. Pennsylvania, under the brand name OSMETTE (or the Osmette model 2007. available from Precision Systems, Inc. Natick MA), for example. En order to achieve an osmolality in the desired range, the concentration of various constituents in the culture medium can be adjusted. Solutes which can be added to the culture medium so as to increase the osmolality thereof include proteins, peptides, amino acids, hydrolyzed animal proteins such as peptones, non-metabolized polymers, vitamins, ions, salts, sugars, metabolites, organic acids, lipids, and the like. In one embodiment, ihe osmolality is controlled by the addition of a peptone to the cell culture along with other components of the culture medium during a fed batch culture procedure. According to the present invention the osmolality is maintained or adjusted to the desired range via the addition of for instance, a basal medium formulation including amino acids, various salts (e.g. NaCl), in addition to a peptone. In a preferred embodiment, the culture medium is supplemented with, for instance, a basal culture medium containing excess amino acids (see, e.g., the "Super" medium of U.S. Patent No. 5,122.469), glucose, and a peptone. It will be appreciated however, that the concentration(s) of other constituents in the culture medium can be modified in order to achieve an osmolality range as set forth above. By controlling either intermittently or continuously the concentration of glucose (the primary energy source), for instance, in the culture medium throughout die culturing, the osmolality of the medium can be maintained at about the desirable range specified. Controlling the glucose concentration serves to provide adequate carbon source to the cells and simultaneously control the production of lactic acid by the host cells. This is advantageous in that it limits the pH decrease in the culture medium which necessitates the addition of a neutralizer(e.£., abase such as Na:C03 or NaOH), which causes the osmolality to rise. The medium can be supplemented to maintain the osmolality within the appropriate margins according to whatever scheme is being used to maintain the cell culture. In a preferred embodiment, the culture system is a fed batch culture system and the medium is supplemented in batch in a feed during the production phase of the cell culture. Additionally the medium can be supplemented during the production phase as described infra. Alternatively, intermittent off-line sampling of the culture medium can be carried out. The osmolality of the culture medium can then be modified by the modulation of a feed solution as required. It will be understood by the skilled artisan that the cell culture procedures of the present invention are selected to achieve the desired level of sialylation of the produced protein. Process parameters in addition to those described herein which influence the degree of sialylation include oxygen level, and glucose level. Culture density, time and storage conditions such as temperature also influence sialylation. The present invention is meant to include those process parameters which are additionally most suitable for enhanced sialylation. III. Recovery of the Glycoprotein Following the polypeptide production phase, the polypeptide of interest is recovered from the culture medium using techniques which are well established in the an. The polypeptide of interest preferably is recovered from the culture medium as a secreted polypeptide, although it also may be recovered from host cell lysates. As a first step, the culture medium or lysate is centrifuged to remove particulate cell debris. The polypeptide thereafter is purified from contaminant soluble proteins and polypeptides, with the following procedures being exemplary of suitable purification procedures: by fractionation on immunoaffinity or ion-exchange columns; ethanol precipitation; reverse phase HPLC; chromatography on silica or on a catjon-exchange resin such as DEAE; chromatofocusing; SDS-PAGE; ammonium sulfate precipitation; gel filtration using, for example, Sephadex G-75; and protein A Sepharose columns to remove contaminants such as IgG. A protease inhibitor such as phenyl methyl sulfonyl fluoride (PMSF) also may be useful to inhibit proteolytic degradation during purification. One skilled in the art will appreciate that purification methods suitable for the polypeptide of interest may require modification to account for changes in the character of the polypeptide upon expression in recombinant cell culture. Especially preferred within the context of the present invention are purification techniques and processes which select for the carbohydrates of the invention. The desired glycoforms of the present invention may be enriched for sialic acid-containing molecules by, for example, ion-exchange soft gel chromatography or HPLC using cation- or anion-exchange resins, wherein the more acidic fraction is collected. IV. Analysis of the Glycoprotein The complex carbohydrate portion of the glycoprotein produced by the processes of the present invention may be readily analyzed if desired, by conventional techniques of carbohydrate analysis. Thus, for example, techniques such as lectin blotting, well-known in the art, reveal proportions of terminal mannose or other sugars such as galactose. Termination of mono-, bi-, tri-, or tetra-antennary oligosaccharide by sialic acids can be confirmed by release of sugars from the protein using anhydrous hydrazine or enzymatic methods and fractionation of oligosaccharides by ion-exchange or size exclusion chromatography or other methods well-known in the an. The pi of the glycoprotein can also be measured, before and after treatment with neuraminidase to remove sialic acids. An increase in pi following neuraminidase treatment indicates the presence of sialic acids on the glycoprotein. The carbohydrate structures of the present invention occur on the protein expressed as N-linked or O-linked carbohydrates. The N-linked and O-linked carbohydrates differ primarily in their core structures, re¬linked glycosylation refers to the attachment of the carbohydrate moiety via GlcNAc to an asparagine residue in the peptide chain. The N-linked carbohydrates all contain a common Manl-6(Manl-3)ManBl-4GlcNAcBl-4GlcNAcB-R core structure. Therefore, in the core structure described, R represents an asparagine residue of the produced protein. The peptide sequence of the protein produced will contain an asparagine-X-serine, asparagine-X-threonine, and asparagine-X-cysteine, wherein X is any amino acid except proline. O-linked carbohydrates, by contrast are characterized by a common core structure, which is the GalNAc attached to the hydroxy! group of a threonine or serine. Of the N-linked, and O-linked carbohydrates, the most important are the complex N- and O-linked carbohydrates. Such complex carbohydrates will contain several antenanary structures. The mono-, bi-, tri,-, and tetra-, outer structures are important for the addition of terminal sialic acids. Such outer chain structures provide for additional sites for the specific sugars and linkages that comprise (he carbohydrates of the instant invention. The resulting carbohydrates can be analyzed by any method known in the art including those methods described herein. Several methods are known in the art for glycosylation analysis and are useful in the context of the present invention. Such methods provide information regarding the identity and the composition of the oligosaccharide attached to the peptide. Methods for carbohydrate analysis useful in the present invention include hut are not limited to lectin chromatography; HPAEC-PAD, which uses high pH anion exchange chromatography to separate oligosaccharides based on charge; NMR; Mass spectrometry; HPLC; GPC; monosaccharide compositional analysis; sequential enzymatic digestion. Additionally, methods for releasing oligosaccharides are known. These methods include 1 )enzymatic, which is commonly performed using peptide-N-glycosidase F/endo-B-galactosidase; 2) p elimination using harsh alkaiine environment to release mainly O-Iinked structures; and 3) chemical methods using anhydrous hydrazine to release both N-and O-linked oligosaccharides Analysis can be performed using the following steps: 1. Dialysis of the sample against deionized water, to remove all buffer salts, followed by lyophilization. 2. Release of intact oligosaccharide chains with anhydrous hydrazine. 3. Treatment of the intact oligosaccharide chains with anhydrous methanolic HCI to liberate individual monosaccharides as O-methyl derivative. 4. N-acetylation of any primary amino groups. 5. Derealization to give per-O-trimethylsilyl methyl glycosides. 6. Separation of these derivative, by capillary GLC (gas - liquid chromatography) on a CP-SIL8 column. 7. Identification of individual glycoside derivatives by retention lime from the GLC and mass spectroscopy, compared to known standards. 8. Quantitation of individual derivatives by FID with an internal standard (13-O-methyl-D-glucose). Neutral and amino-sugars can be determined by high performance anion-exchange chromatography combined with pulsed amperometric detection (HPAE-PAD Carbohydrate System, Dionex Corp.). For instance, sugars can be released by hydrolysis in 20% (v/v) trifiuoroacetic acid at 100°C for 6 h. Hydrolysates are then dried by Iyophiliiation or with a Speed-Vac (Savant Instruments). Residues are then dissolved in 1% sodium acetate trihydrate solution and analyzed on a HPLC-AS6 column as described by Anumula et al. (Anal. Biochem. 195:269-280(1991). Sialic acid can be determined separately by the direct colorimetric method of Yao et al- (Anal Biochem. 179:332-335 (1989)) in triplicate samples. In a preferred embodiment the thiobarbaturic acid (TBA) of Warren, L. J. Biol Chem 238:(8) (1959) is used. Alternatively, itnmunoblot carbohydrate analysis may be performed. According to this procedure protein-bound carbohydrates are detected using a commercial glycan detection system (Boehringer) which is based on the oxidative immunoblot procedure described by Haselbeck and Hosel [Haselbeck et al. Glycoconjugate J., 7:63 (1990)]. The staining protocol recommended by the manufacturer is followed except that the protein is transferred to a polyvinylidene difluonde membrane instead of nitrocellulose membrane and the blocking buffers contained 59c bovine serum albumin in 10 mM iris buffer. pH 7.4 with 0.99c sodium chloride. Detection is made with anti-digoxigenin antibodies linked with an alkaline phosphate conjugate (Boehringer). 1:1000dilution in Iris buffered saline using the phosphatase substrates. 4-nitroblue tetrazolium chloride. 0.03% (w/v) and 5-bromo-4 chloro-3-mdoyl-phosphate 0.03% (w/v) in 100 mM iris buffer, pH 9.5, containing 100 mM sodium chloride and 50 mM magnesium chloride. The protein bands containing carbohydrate are usually visualized in about 10 to 15 min. The carbohydrate may also be analyzed by digestion with peptide-N-glycosidase F. According to this procedure the residue is suspended in 14 fi] of a buffer containing 0.18% SDS. 18 mM beta-mercaptoethanol. 90 mM phosphate, 3.6 mM EDTA, at pH 8.6. and heated at 100 °C for 3 min. After cooling to room temperature, the sample is divided into two equal pans. One aliquot is not treated further and serves as a control. The second fraction is adjusted to about I % NP-40 detergent followed by 0.2 units of peptide-N-glycosidase F (Boehringer). Both samples are warmed at 37° C for 2 hr and then analyzed by SDS-polyacrylamide gel electrophoresis. V. Tumor Necrosis Factor Receptor-Immunoglobulin Chimeras In a preferred embodiment the processes of the present invention are used to produce tumor necrosis factor receptor (TNFRHmmunoglobhm. (Igl chimeras. Especially preferred among this class of chimeric proteins is the soluble type 1 TNFR IgG, The TNFRl-IgG, chimeras produced are useful in the treatment or diagnosis of many TNF-mediated or TNF-retated diseases and disorders. The term "treatment" in this context includes both prophylactic (prevention), suppression (e.g., of a symptom) and treatment of an existing condition. The pathologic conditions associated with TNF include but are not limited to gram negative and gram positive bacteremia, endotoxic shock, graft rejection phenomena, rheumatoid arthritis, systemic lupus, Crohn's disease and other autoimmune and inflammatory diseases associated with TNF. The TNFRl-IgG, preparations of the instant invention are useful generally in those indications where monoclonal antibodies to TNF have been found to be useful. For instance, in animal models, monoclonal antibodies to TNF-aipha were found to have protective effect when employed prophylaciically (Tracey, KJ. et ai, (1987) Nature 330:662). In a phase I clinical study reported by Exley, A.R. et al., (1990) Lancet 335:1275, a murine monoclonal antibody to recombinant human TNF-aipha was found to be safe when administered to human patients with severe septic shock. TNFRl-IgG, is suitably used in the treatment of rheumatoid arthritis as well as septic shock. In a method of treating a disease or disorder associated with TNF a therapeutically active amount of TNFRl-IgG, preparation is administered to a subject in need of such treatment. The preferred subject is a human. An effective amount of a TNFR 1-IgG, glycoform preparation of the instant invention for the treatment of a disease or disorder is in the dose range of 0.01-100 mg/patient; preferably 1 mg-75 mg/patient and most preferably between about 10 and about 50 mg/patient. For administration, the TNFRl-IgG, preparation should be formulated into an appropriate pharmaceutical or therapeutic composition. Such a composition typically contains a therapeutically active amount of the TNFRl-IgG, preparation and a pharmaceutical^ acceptable excipient or carrier such as saline, buffered saline, dextrose, or water. Compositions may also comprise specific stabilizing agents such as sugars, including mannose and mannitol, and local anesthetics for injectable compositions, including, for example, lidocaine. The present invention provides compositions which further comprise a therapeutically active amount of an additional active ingredient such as monoclonal antibodies (e.g. anti-TNF antibodies, antibodies to Mac 1 or LFA 11 or other receptors associated with TNF production, e.g. !L-1 or IL-2 receptors, etc. A preferred therapeutic composition for single or combined therapy, as above, comprises a novel TNFRl-[gG, preparation of this invention which exhibits prolonged clearance from the blood while retaining significant functional activity. Such a prolonged functional half-life permits simplified, bolus-dose administration and contributes to potency in vivo. Preferred TNFRl-IgG, chimeras in the therapeutic composition include the TNFRl-IgG, and preparations described herein, for example: (1) TNFRl-IgG, preparations comprising a complex oligosaccharide terminated by one or more residues of a sialic acid; (2) TNFRl-Igd preparations wherein the range of isoelectric point, pi, of the preparation is between 5.5 and 7.5 as determined by chromatofocusing, in which the pi is sensitive to neuraminidase treatment; (3) TNFRl-IgG, preparations having about 1-2 moles of exposed N-acetylglucosamine residues per mole of protein. (4) TNFR1 -IgG, preparations having a molar ratio of sialic acid to protein of about 4-7, especially about 5-6. (5) TNFR 1 -IgG, preparations having a molar ratio of sialic acid to N-acetylglucosamine of about 0.35 to about 0.5, and more preferably of about 0.4 to about 0.45. Routes of administration for the individual or combined therapeutic compositions of the present invention include standard routes, such as, for example, intravenous infusion or bolus injection. Also provided is the use of a TNFRl-IgG, preparation of this invention in the manufacture of a medicament for the treatment of a human or animal. Having now generally described the invention, the same will be more readily understood through reference to me following examples which are provided by way of illustration, and are not intended to be limiting of the present invention, unless specified. Examples The biological effects of TNF-alpha and TNF-beta are mediated through specific receptors (Dembic et at- (1990) Cytokines, 2:231). Molecular cloning has demonstrated the existence of two distinct types of TNF receptors (TNFR) with apparent molecular masses of 55kD (type 1) (Schall et at., (1990) Cell 61:361) and 75 kD (type 2) (Smith et ai, (1990) Science 248:1019), each of which naturally binds lo both TNF alpha and TNF beta (Loetscher et at., (1990) Cell, 61:351; Shall et ai (1990) Cell, 61:361; Kohno et at., (1990) Proc. Natl. Acad. Sci. USA 87:8331). The extracellular portions of both receptors are found naturally as soluble TNF binding proteins (Kohno et ai, supra). TNF agonists have been created which block the deleterious effect of TNF in various immune and inflammatory events (Peppel et ai, (1991) J. Exp. Med., 174:1483-1489; Ulich (1993) Am. J. Path., 142:1335-1338; Howard, O.M.Z., (1993) Proc. Natl. Acad. Sci. USA 90:2335-2339; Wooley, P.H., (1993) J. Immunol. 151:6602-6607). One such agonist (Werner ef ai, (1991) J. Cell. Biochem. Abstracts. 2U(h annual meeting, p. 115) combines the extracellular domain of human 55 kD type I TNFR with a portion of the hinge and Fc regions of human immunoglobulin G1 heavy chain. In this Example mammalian ceils transfected with a vector containing the cDNA encoding the TNFR I -IgG, chimera were cultured. Methods A. Cell Line The Chinese hamster ovary (CHO)celi line used as the mammalian host cell line was derived from CHO-K1 (ATCC No. CCL61 CHO-K1). A CHO-K1 mutant dihydrofolate reductase (DHFR) deficient cell line named CHO-Kl DUX-B11 (DHFR) (obtained from Dr. L. Chasin of Columbia University; Simonsen. C.C., and Levinson, A.D., (1983) Proc. Natl. Acad- Sci. USA 80:2495-2499; Urlaub G.. and Chasin, L., (1980) Proc. Nail. Acad. Sci. USA 77:4216-4220) was then used to obtain a cell line with a reduced requirement for insulin by transfection with the vector containing the cDNA fortiie prepromsulin (Surest at., (1980) Science, 208:57-59). The selected clone designated dpl2.CHO requires glycine, hypoxanthine, and thymidine for growth, thus verifying their DHFR genotype. B. Construction of Soluble Type 1 TNFR-lgG, Chimera A soluble type 1 TNFR-IgG( chimera was constructed by gene fusion of the extracellular domain of human type 1 TNFR with the hinge region and CH2 and CH3 domains of IgG, heavy chain (further referred to asTNFRl-IgG,). The human type 1 TNFR encoding DNA sequence (see Loetscher et ai., Supra) was obtained from the plasmid pRK-TNF-R [Schall exal. Cell 61., 361 (1990)]. To construct this starting plasmid, a 2.1kb placental cDNA clone (Schall et at. Supra) was inserted into the mammalian expression vector pRK5. the construction of which is described in EP Pub. No. 307,247, published 15 March 1989. This cDNA starts at nucleotide position 64 of the sequence reported by Loetscher et ai, with die initiating methionine 118 bp downstream. The source of the IgG, encoding sequence was the CD4-IgG expression plasmid pRrCCD4,Ftl [Capon, D.J. et al„ Nature 337.525 (1989); Bym et ai. Nature 344.667 (1990)], containing a cDNA sequence encoding a hybrid polypeptide consisting of residues 1-180 of the mature human CD4 protein (two N-terminal CD4 variable domains) fused to human IgG, sequences beginning ataspartic acid 216 (taking amino acid 114 as the first residue of the heavy chain constant region [Kabai etai, Sequences of Proteins of Immunological Interest 4th edition (1987)] which is me first residue of the IgG, hinge after the cysteine residue involved in heavy-light chain bonding), and ending with residue 441 to include the CH2 andCH3 Fc domains of IgG,- TNFRl-IgG, was constructed by generating restriction fragments of plasmids pRK-TNF-R and pRKCD4jFcl and ligating them, using deletional mutagenesis, so that threonine residue 171 of mature TNFR's is juxtaposed to aspartic acid residue 216 of IgG, heavy chain [Kabat et ai, Supra], The resulting plasmid pRKTNFR-IgG contained the full length coding sequence for TNFR, IgG,. C. Cell Culture The gene encoding me soluble type 1 TNFR-lgG, was introduced into dpl2.CHO cells by transfection. This was accomplished using the calcium phosphate technique for introducing DNA into mammalian cells. Two days following transfection me cells were trypsinized and replated into selective medium (glycine-hypoxanthine and thymidine free Ham's F-12 DMEM, 1:1 v/v with 2% dialyzed serum). Subsequent isolates were screened for secretion of TNFRl-IgG,. Clones expressing TNFRl-IgG, where amplified in methotrexate yielding high ;xpressing clones and subsequently adapted to serum free medium. These cells were under a continuous selective pressure until transferred to nonselective medium for growth and expansion of the inoculum. To provide cells for TNFRl-IgG, production cultures the cell population described above was expanded from the medium containing methotrexate by serial subcultivations in vessels of increasing volumes to growth medium containing no methotrexate. For these steps of the process the non selective growth medium was DMEM/HAM F-12 based formulation (see U.S. Patent 5.122.469, for example) with modified concentrations of some components, such as glucose, amino acids, salts, sugar, vitamins glycine, hypoxanthine, and thymidine; recombinant human insulin, hydrolyzed peptone (Primatone HS or Primatone RL), a cell protective agent such as Pluronic F68 (pluronic polyol) or the equivalent; Gentamycin; Lipid and trace elements. The cultures were controlled at pH 7.2 +/• 0.4 by the use of CO; gas (acid) and /or Na.CO, (base). Temperature was controlled near 37 3C during the growth period. Dissolved oxygen was maintained above 5% of air saturation by direct sparging with air and/or oxygen gas. The osmolality during the inoculum expansion phase was maintained between about 250 mOsm and 350 mOsm. The growth phase for each culture was followed by a second phase or transition phase wherein culture parameters were changed from optimal growth to production conditions. During this transition phase the temperature of the culture system was decreased, generally to about between 30 and 35 °C. Butyrate was added and a certain osmolality range was engaged. Product accumulated during this production phase was analyzed for sialic acid content. In a typical production schedule approximately 1.2 x 106 cells derived from the inoculum expansion from the selective stage where grown in a growth phase with a starting osmolality of 300 mOsm. The growth medium was supplemented with trace elements, recombinant human insulin and hydrolyzed peptone. The cells were grown under these condition for 2 days. At the start of the third day the temperature of the ceil culture was lowered. Simultaneous to or following the temperature shift, sodium butyrate was added to the cell culture and the desired production osmolality was engaged by addition of various media components. Cells were grown under these conditions with feeding for 9-10 days. The cells were fed when necessary with various media components. Table I describes the production conditions for various production processes. D. Recovery of the TNFR-leG The TNFRl-IgG, chimera was purified to greater than 95% homogeneity by affinity chromatography on immobilized Staphylococcus aureus Protein A as described by Capon et at.. Supra. E. Carbohydrate Analysis Sialic acid content was analyzed by the method of Warren, L. (1959) J. Biol. Chem. 234:1971-1975. Results Cell specific productivity for each of the production cultures described in Table I was plotted against the sialic acid content of the harvested product. The results are presented in Figure I. The highest sialic acid content was observed when process parameters where maintained at a production osmolality of between about 360 and 420 mOsm. and a butyrate concentration of about 1 mM. Sialic acid content could be controlled over a wide range of values by adjusting the process parameters. Example II Plasma pharmacokinetic half-lives and/or clearance rates of different preparations were determined. The preparations containing a higher sialic acid content in general exhibited increased plasma half-life and/or lower total clearance rates compared to preparations containing a decreased sialic acid content. Methods Seventeen male Sprague-Dawley-derived rats weighing 272-315 g were randomly assigned to one of three TNFRl-IgG, fusion protein treatment groups (N=5 or 6 per group). The animals were injected intravenously with a nominal dose of 5 mg/kg of test material via a femoral vein cannula. The test materials chosen included two TNFRl-IgG, preparations from a process in which the butyrate concentration during the production phase was 6mM and the osmolality during the production phase was maintained at about 400 mOsm, Process I, and a third TNFRl-IgG, preparation from a process in which the butyrate concentration was 12 mM and the osmolality during the production phase was about 500 mOsm, Process II. Two mL blood samples were collected onto EDTA (8,5 %) pre-dose, and at 5 min, and 2, 6, 10,24, 34, 48,56, 72,96, and 120 hours post- dose. Blood samples were cenirifuged. plasma harvested, and the samples analyzed for concentrations of TNFRI-lgG| fusion protein. An enzyme-linked immunological and biological binding assay (ELIB A) was used to quantitate TNFR1-IgGi in rat plasma. This assay is based on the ability of TNF-alpha coupled to horseradish peroxidase (TNF-alpha-HRP) to bind to the receptor portion of the TNFRl-IgG, fusion protein. In this assay, Fab fragments of goat ami human (gGFc coated on wells of microliter plates were used to capture TNFRl-IgG, by interaction with the Fc portion of the molecule. TNF-alpha-HRP was added to the wells and allowed to bind to the receptor portion of the captured TNFRl-IgG,. Quantification was determined by measurement of a color produced by reaction of peroxidase with peroxide and an onho-phenylenediamine (OPD) substrate. The range of the assay is 0.003 lo 0.02 mg/mL. The presence of EDTA in the plasma samples was shown to have no effect on the performance of the assay. The dose was normalized to account for dosing solution concentration differences. All calculations were based on concentration versus time data through the 120-hr limepoint. Truncated area under the curve (AUQia,) was calculated using the trapezoidal rule arid weight-normalized truncated clearance (Cl^-o/W") was calculated as dose/A"UC0.|20. Results Clearance rates varied depending upon the process version utilized (Process 1 compared to Process II) and the amount of sialic acid present in the product. Clearance rates for individual animals and the resulting mean and standard deviations from this study are presented in Table II below. Process I exhibits a slower and more favorable clearance rate. Example III MONOSACCHARIDE COMPOSITION OF TNFRMeG, Determination of oligosaccharide carbohydrate composition and structure of TNFRl-IgG,, prepared as described in Example ! showed that the sialic acid content of the various process version varied. Rapid plasma clearance was associated with high exposed GlcNAc, lower sialic acid on the oligosaccharide chains and (by inference) accessibility of the protein to mannose or galactose receptors. Slow plasma clearance was associated with more terminal sialic acid residues. A. Sources of Test Material TNFRl-IgG, was produced according to the methods outlined as described in Example 1 above. Process I material was obtained from eel] culture using 6mM butyrate and an osmolality of about 400 mOsm during the production phase. Process material II was obtained from cell culture using 12 mM butyrate and an osmolality of about 500 mOsm during the production phase. B. Methods Release of intact neutral and amino-sugars was determined by high pH anion-exchange chromatography combined with pulsed amperometric detection. Analysis was performed using the following steps: 1. Buffer exchange was performed with TNFRl-IgG, (approximately 50 ug/ml) and the appropriate reference samples so that the final sample was contained in 1% acetic acid. 2 Approximately 90 ug TNFRl-IgG, as well as samples of reference materials were frozen in a dry ice and alcohol bath and the frozen sample lyophilized overnight. 3. The freeze dried samples were reconstituted in 500pl trifluoroacetic acid and incubated at 120° C fori hour. 4. After acid hydrolysis the TNFRl-IgG, and reference samples were cooled and evaporated to dryness. 5. Samples were reconstituted with water to a final concentration of approximately 0.6 mg/ml. 6. Separation of the monosaccharides was performed at ambient temperature by high pH anion exchange chromatography with pulsed amperometric detection using a Dionex CarboPac PA I (4x250 mm) column (Dionex Corp., Sunnyvale, CA). The results presented in Table IV and V demonstrate that ihe composition of the TNFRl-IgG, are typical of sialic acid terminated mono- bi- and triantennary complex oligosaccahrides. It can be concluded that the TNFRl-IgG, prepared by Process I contains a significantly higher proportion of sialic acid and a significantly lower proportion of exposed GlcNAc. Sialic acid per mole protein indicates thai this material would have a lower isoelectric point than the material produced by Process II. By comparison with the results in Table II, these results also indicate that the slower plasma clearance of Process I material correlates with lower exposed GlcNAc and generally with a higher sialic acid content. Table IV and V demonstrate TNFRl-IgG, preparations comprising a complex oligosaccharide terminated by one or more residues of a sialic acid. The preferred TNERI-IgG, preparations comprise TNFRl-IgG 1 molecules having about 1 -2 moles of exposed N-acetylglucosamine residues per mole of protein. The molar ratio of sialic acid to protein is about 4-7. The TNFR1 -IgGj preparations have a molar ratio of sialic acid to N-acetylglucosamine of about 0.4 to about 0.45. Example IV The pi of the heavily sialylated preparation is lower than the pi of the lightly sialylated preparation. Methods Isoelectric focusing was performed for die various preparation described in Example II. The isoelectric focusing gels separate the glycoproteins of the preparation according to their isoelectric point, pi, using a pH gradient created with ampholytes of different pH. In this study, analysis of the preparations was performed using a pH gradient of 10 to 4. Results The TNFR 1-IgG, preparations exhibit an isoelectric point range, of about 5.5 to about 7.5 as determined by chromatofocusing, in which the pi is sensitive to neuraminidase treatment. The references cited above are all incorporated by reference herein, whether specifically incorporated or not. While this invention has been described in connection with specific embodiments thereof, it will be understood that it is capable of further modifications. This application is intended to cover any variations, uses, or adaptations of the inventions following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains and as may be applied to the essential features hereinbefore set forth as follows in the scope of the appended claims. WE CLAIM: 1. A process for controlling the amount of sialic acid present on an oligosaccharide side chain of a glycoprotein produced by mammalian cell culture which comprises: culluring the mammalian host cell in a production phase of the culture which is characterized by; i) adding an alkanoic acid or a sah thereof to the cell culture at a concentration of 0.1 mJVTto 20 mM; and ii) maintaining the osmolality of the cell culture at 250 to 600 mOsrri. 2. The process as claimed in claim 1 wherein the amount of sialic acid present on the oligosaccharide side chain of the glycoprotein is increased and wherein; cell specific productivity of the cell culture is decreased by culturing the host cell concentration of the alkanoic acid or salt thereof of 0.1 to 6mM and maintaining the osmolality at 300-450 mOsm. 3. The process as claimed in claim 2 in which the host cell is a CHO cell. 4. The process as claimed in claim 3 wherein the alkanoic acid or salt thereof is sodium butyrate. 5. The process as claimed in claim 4 wherein the glycoprotein produced is a mammalian glycoprotein. 6. The process as claimed in claim 5 wherein the glycoprotein is a tumor necrosis factor receptor immunoglobulin chimera. 7. The process as claimed in claim 6 in which the host cell is a dp 12.CHO cell line transfected with the vector carrying the cDNA encoding a soluble type 1 tumor necrosis factor immunoglobulin Gj chimera. ■/—- .' 8.) The process as claimed in claim 1 wherein the amount of sialic acid present on the oligosaccharide side chain of the glycoprotein is decreased and wherein; cell specific productivity of the cell culture is increased by culturing the host cell at a concentration of 6mM to 12 mM of the alkanoic acid or salt thereof and maintaining the osmolality at 450-600 mOsm. 9. The process as claimed in claim 8 in which the host cell is a CHO cell. 10. The process as claimed in claim 9 wherein the alkanoic acid or salt thereof is sodium butyrate. U. The process as claimed in claim 10 wherein the glycoprotein produced is a mammalian glycoprotein. 12. The process as claimed in claim II wherein the glycoprotein is a tumor necrosis factor receptor immunoglobulin chimera. 13. The process as claimed in claim 14 in which the host cell is a dp 12.CHO cell line cell transfected with the vector carrying the cDNA for a soluble type I tumor necrosis factor immunoglobulin G| TNFRI-IgG] chimera. 14 J A process for producing a tumor necrosis factor receptor-imniunogiobulin TNFRI-lgC chimeric protein comprising; (a) culturing a mammalian host cell which expresses a TNFR-IG chimera in a growth phase under such conditions and for a period of time such that maximum cell growth is achieved: (b) culturing the host cell in a production phase in the presence of sodium butyrate at a concentration of 6mM to 12 mM: (C) maintaining the osmolality of the production phase at 450-600 mOsm. 15.) A process for producing a tumor necrosis factor receptor-immunogiuhulin TNFRI-lgG, chimeric protein comprising; (a) culturing a mammalian host cell which expresses a TNFR-IG chimera in a growth phase under such conditions and for a period of time such that maximum cell growth is achieved; (b) culturing the host cell in a production phase in the presence of sodium hutyrate at a concentration of ImM to 6 mM: (c) maintaining the osmolaHty of the production phase at 300-450 mOsm. 16. The process as claimed in claim 15 in which the host cell is a CHO cell. 17. The process as claimed in claim 16 in which the host cell is a dp 12,CHO line. 18. A preparation comprising the TNFRl-IgG, produced by the process as claimed in claim 17, 19. A therapeutic composition comprising the TNFRI-IgG,produced by the process as claimed in claim 15 and a pharmaceutically acceptable exclpient. 20. A TNFRI-IgGl preparation comprising TNFRI-IgGl molecules wherein the TNFRI-IgGl molecules have a molar ratio of sialic acid to protein of 4-7. 21. A TNFR 1-IgGl preparation comprising TNFR I-IgG 1 molecules wherein the TNFRl-IGl molecules have about 1-2 moles of exposed N-acetylglucosam\ne residues per mole of TNFR 1 -IgGl protein. 22. A TNFRI-IgGl preparation comprising TNFRI-IgGl molecules wherein the TNFRI-IgGl molecules have a molar ratio of sialic acid to N-acetylglucosamine of 0.35 to 0.5. 23. The TNFRl-lgGl preparation as claimed in claim 22 wherein the molar ration of sialic acid to N-acetylglucosamine is 0.39 to 0.45.

Documents:

0979-mas-1996 abstract-duplicate.pdf

0979-mas-1996 abstract.pdf

0979-mas-1996 assignment.pdf

0979-mas-1996 claims-duplicate.pdf

0979-mas-1996 claims.pdf

0979-mas-1996 correspondence-others.pdf

0979-mas-1996 correspondence-po.pdf

0979-mas-1996 description (complete)-duplicate.pdf

0979-mas-1996 description (complete).pdf

0979-mas-1996 drawings-duplicate.pdf

0979-mas-1996 drawings.pdf

0979-mas-1996 form-1.pdf

0979-mas-1996 form-10.pdf

0979-mas-1996 form-19.pdf

0979-mas-1996 form-26.pdf

0979-mas-1996 form-3.pdf

0979-mas-1996 form-4.pdf

0979-mas-1996 form-6.pdf

0979-mas-1996 others.pdf

0979-mas-1996 petition.pdf