Title of Invention	NOVEL 14 AND 15 MEMBERRED RING COMPOUNDS
Abstract	The present invention relates to 14- or 15-mem- bered macrolides substituted at the 4" position of formula (I) and pharmaceutically acceptable derivatives thereof, to processes for their preparation and their use in therapy or prophylaxis of systemic or topical microbial infections in a human or animal body.

Full Text

R4 and R5 taken together with the Intervening atoms form a cyclic grogp having the following structure: H3C wherein Y is a bivalent radical selected from -CH2-, -CH(CN)-, -0-, -N(R13)- and - CH(SR13)-; R6 is hydrogen or fluorine; R7 is hydrogen or C1-6alkyl; R8 and R9 are each independently hydrogen, C1-6alkyl, -C(=NR10)NR14R15 or - C(0)R14 or R8 and R9 together form =CH(CR14R15)faryl, =CH(CR14R15)fheterocyclyl, =CR14R15 or =C(R14)C(0)OR14, wherein the alkyl, aryl and heterocyclyl groups are optionally substituted by up to three groups independently selected from R16; R10 is -OR17, C1-6alkyl. -(CH2)garyl, -(CH2)gheterocyclyl or -(CH2)hO(CH2)lOR7. wherein each R10 group is optionally substituted by up to three groups Independently selected from R16; R11 is a heterocyclic group having the following structure: or R12 is hydrogen or C1-6alkyl; R13 IS hydrogen or C1-4alky! optionally substituted by a group selected from optionally substituted phenyl, optionally substituted 5 or 6 membered heteroaryl and optionally substituted 9 to 10 membered fused bicyclic heteroaryl; R14 and R15 are each independently hydrogen or C1-6alkyl; R16 is halogen, cyano, nitro, trifluoromethyl, azido, -C(0)R21, -C(0)OR21, -OC(0)R21, - OC(0)OR21, -NR22C(O)R23, -C(O)NR22R23, -NR22R23, hydroxy, C1-6alkyl, -S(O)kC1. WO 2004/101587 PCT/EP2004/005083 6alkyl, C1-6alkoxy, -(CH2)maryl or -(CH2)mheteroaryl, wherein the alkoxy group is optionally substituted by up to three groups independently selected from -NR14R15, halogen and -OR14, and the aryl and heteroaryl groups are optionally substituted by up to five groups independently selected from halogen, cyano, nitro, trifluoromethyl, azido, - 5 C(0)R24, -C(0)OR24, .OC(0)OR24, -NR25C(0)R26, -C(0)NR25R26 -NR25R26, hydroxy, C1-6alkyl and C1-6alkoxy; R17 is hydrogen, C1-6alkyl, C3-7cycloalkyl, C3-6alkenyl or a 5 or 6 membered heterocyclic group, wherein the alkyl, cycloalkyl, alkenyl and heterocyclic groups are optionally substituted by up to three substituents independently selected from optionally 10 substituted 5 or 6 membered heterocyclic group, optionally substituted 5 or 6 membered heteroaryl, -OR27, -S(0)nR27, -NR27R28 CONR27R28 halogen and cyano; R18 is hydrogen, -C(0)OR29, -C(0)NHR29, -C(0)CH2N02 or-C(0)CH2S02R7; R19 js hydrogen, C1-4alkyl optionally substituted by hydroxy or C1-4alkoxy, C3. 7cycloaikyl, or optionally substituted phenyl or benzyl; 15 R20 is halogen, C1-4alkyl, C1-4thioalkyl, C1-4alkoxy, -NH2, -NH(C1-4alkyl) or -N(C1_ 4alkyl)2; R21 is hydrogen, C1-10alkyl. -(CH2)paryl or-(CH2)pheteroaryl; R22 and R23 are each independently hydrogen, -OR14, C1-6alkyl,-(CH2)qaryl or - (CH2)qheterocyclyl; 20 R24 is hydrogen, C1-10alkyl, -(CH2)raryl or -(CH2)rheteroaryl; R25 and R26 are each independently hydrogen, -OR14, C1-6alkyl, -(CH2)saryl or - (CH2)sheterocyclyl; R27 and R23 are each independently hydrogen, C1-4alkyl or C1-4alkoxyC1-4alkyl; R29 IS hydrogen, 25 C1-6alkyl optionally substituted by up to three groups independently selected from halogen, cyano, C1-4alkoxy optionally substituted by phenyl or C1-4alkoxy, - C(0)C1-6alkyl, -C(O)OC1-6alkyl. -OC(O)C1-6alkyl, -OC(O)OC1_6alkyl, - C(0)NR32R33, -NR32R33 and phenyl optionally substituted by nitro or -C(O)OC1. 6alkyl, 30 -(CH2)wC3-7cycloalkyll "(CH2)wheterocyclyl, -(CH2)wheteroaryl, -(CH2)waryl, C3-6alkenyl, or 35 C3-6alkynyl; R30 is hydrogen, C1-4alkyl, C3-7cycloalkyl, optionally substituted phenyl or benzyl, acetyl or benzoyl; R31 is hydrogen or R20, or R31 and R19 are linked to form the bivalent radical -0(CH2)2- or-(CH2)r; 40 R32 and R33 are each Independently hydrogen or C1-6alkyl optionally substituted by phenyl or -C(0)OC1-6alkyl, or WO 2004/101587 PCT/EP2004/005083 R32 and R33, together with the nitrogen atom to which they are bound, form a 5 or 6 membered heterocyclic group optionally containing one additional heteroatom selected from=oxygen, nitrogen and sulfur; X is-U(CH2)v-; 5 U is a divalent radical selected from -N(R30)-, -0-, -S(0)zr, -N(R30)C(O)-, -C(0)N(R30)- and-N[C(O)R30]-; W is -C(R31)- or a nitrogen atom; d is an integer from 1 to 5; e is an integer from 2 to 4; 10 f, g, h, m, p, q, r, s and w are each independently integers from 0 to 4; i is an integer from 1 to 6; J, k, n and z are each independently integers from 0 to 2; t is 2 or 3; v is an integer from 1 to 8; 15 and pharmaceutical acceptable derivatives thereof. According to another embodiment the present invention provides compounds of general formula (IA): 20 wherein 25 A is a bivalent radical selected from -C(0)-, -C(0)NH-, -NHC(0)-, -N(R7)-CH2-, -CK2- N(R7)-, -CH(NR8R9)-.and -C(=NR10)-; R1is-OC(0)(CH2)dXR11; R2 is hydrogen or a hydroxyl protecting group; R3 is hydrogen, C1-4alkyl, or C3-6alkeny optionally substituted by 9 to 10 membered 30 fused bicyclic heteroaryl; R4 is hydroxy, C3-6alkenyloxy optionally substituted by 9 to 10 membered fused bicyclic heteroaryl, or C1-6alkoxy optionally substituted by C1-6alkoxy or -O(CH2)eNR7R12, WO 2004/101587 PCT/EP2004/005083 R° is hydroxy, or R4 and R5 taken together with the intervening atoms form a. cyclic group having the following, structure: wherein Y is a bivalent radical selected from -CH2-. -CH(CN)-, -0-, -N(R13)- and - CH(SR13)-: R6 is hydrogen or fluorine; R7 is hydrogen or C1-6alkyl; R8 and R9 are each independently hydrogen, C1-6alkyl, -C(=NR10)NR14R15 or - C(0)R14, or R8 and R9 together form =CH(CR14R15)faryl, =CH(CR14R15)fheterocyclyl, =CR14R15 or =C(R14)C(O)OR14, wherein the alkyl, aryl and heterocyclyl groups are optionally substituted by up to three groups independently selected from R16; R10 is -OR17 C1-6alkyl, -(CH2)garyl, -(CH2)gheterocyclyl or -(CH2)hO(CH2)iOR7, wherein each R10 group is optionally substituted by up to three groups independently selected from R16; R11 is a heterocyclic group having the following structure: or R12 is hydrogen or C1-6alkyl; R13 is hydrogen or C1-4alkyl optionally substituted by a group selected from optionally substituted phenyl, optionally substituted 5 or 6 membered heteroaryi and optionally substituted 9 to 10 membered fused bicyclic heteroaryi; R14 and R15 are each independently hydrogen or C1-6alkyl; WO 2004/101587 PCT/EP2004/005083 R16 is halogen, cyano, nitro, trifluoromethyl, azido, -C(O)R21, -C(O)OR21, -OC(O)R21, - OC(O)OR21, -NR22C(O)R23, -C(O)NR22R23, -NR22R23, hydroxy, C1-6alkyl, -S(O)kC1. 6alkyl, C1-6alkoxy, -(CH2)maryl or -(CH2)mheteroaryl, wherein the alkoxy group is optionally substituted by up to three groups independently selected from -NR14R15, 5 halogen and -OR14, and the aryl and heteroaryl groups are optionally substituted by up to five groups independently selected from halogen, cyano, nitro, trifluoromethyl, azido, - C(O)R24, -C(O)OR24, -OC(O)OR24, -NR25C(O)R26, -C(O)NR25R26, -NR25R26, hydroxy, C1-6alkyl and C1-6alkoxy; R17 is hydrogen, C1-6alkyl, C3-7cycloalkyl, C3-6alkenyl or a 5 or 6 membered 10 heterocyclic group, wherein the alkyl, cycloalkyl, alkenyl and heterocyclic groups are optionally substituted by up to three substituents independently selected from optionally substituted 5 or 6 membered heterocyclic group, optionally substituted 5 or 6 membered heteroaryl, -OR27, -S(O)nR27, -NR27R28, -CONR27R28, halogen and cyano; R18 is hydrogen, -C(O)OR29, -C(O)NHR29 or -C(O)CH2NO2; 15 R19 is hydrogen, C1-4alkyl optionally substituted by hydroxy or C1-4alkoxy, C3- 7cycloalkyl, or optionally substituted phenyl or benzyl; R20 is halogen, C1-4alkyl, C1-4thloalkyl, C1-4alkoxy, -NH2, -NH(C1-4alkyl) or -N(C1_ 4alkyl)2; R21 is hydrogen, C1-10alkyl, -(CH2)paryl or -(CH2)pheteroaryl; 20 R22 and R23 are each independently hydrogen, -OR14, C1-6alkyl, -(CH2)qaryl or - (CH2)qheterocyclyl; R24 is hydrogen, C1-10alkyl, -(CH2)raryl or-(CH2)rheteroaryl; R25 and R26 are each independently hydrogen, -OR14, C1-6alkyl, -(CH2)sary' or - (CH2)sheterocyclyl; 25 R27 and R28 are each independently hydrogen, C1-4alkyl or C1-4alkoxyC1-4alkyl; R29 IS hydrogen, C1-6alkyl optionally substituted by up to three groups independently selected from halogen, C1-4alkoxy, -OC(O)C1-6alkyl. -OC(O)OC1-6alkyl, -C(O)NR32R33 and -NR32R33, -(CH2)wC3-7cycloalkyl, C3-6alkenyl or C3-6alkynyl; R30 IS hydrogen, C1-4alkyl, C3-7cycloalkyl, optionally substituted phenyl or benzyl, acetyl 30 or benzoyl; R31 is hydrogen or R20, or R31 and R19 are linked to form the bivalent radical -O(CH2)2- or-(CH2)t-; R32 and R33 are each independently hydrogen or C1-6alkyl optionally substituted by - C(O)OC1-6alkyl, or 35 R32 and R33, together with the nitrogen atom to which they are bound, form a 5 or 6 membered heterocyclic group optionally containing one additional heteroatom selected from oxygen, nitrogen and sulfur; X is-U(CH2)v; U is a divalent radical selected from -N(R30)-, -O-, -S(O)z-, -N(R30)C(O)-, -C(O)N(R30)- 40 and-N[C(O)R30]-; W is -C(R31)- or a nitrogen atom; d is an integer from 1 to 5; WO 2004/101587 PCT/EP2004/005083 e is an integer from 2 to 4; f. 9. h, m, p, q, r, s and w are each independently integers from 0 to 4; i is an integer from 1 to 6; J, k, n and z are each independently integers from 0 to 2; t is 2 or 3; v is an integer from 2 to 8; and pharmaceutically acceptable derivatives thereof. According to a further embodiment the present invention provides compounds of general formula (IB): CH3CH2. H3C OCH3 (IB) wherein A is a bivalent radical selected from -C(O) -C(O)NH-, -NHC(O)-, -N(R7)-CH2-, -CH2- N(R7)-, -CH(NR8R9)- and -C(=NR10); R1 is-OC(O)(CH2)dXR11; R2 is hydrogen or a hydroxyl protecting group; R3 is hydrogen, C1-4alkyl, or C3-6alkenyl optionally substituted by 9 to 10 membered fused bicyclic heteroaryl; R4 is hydroxy, C3-6alkenyloxy optionally substituted by 9 to 10 membered fused bicyclic heteroaryl, or C1-6alkoxy optionally substituted by C1-6alkoxy or -O(CH2)eNR7R12, R5 Is hydroxy, or R4 and R5 taken together with the intervening atoms form a cyclic group having the following structure: 11 12 H3C WO 2004/101587 PCT/EP2004/005083 wherein Y is a bivalent radical selected from -CH2-, -CH(CN)-, -O-, -N(R13) and - CH(SR13)-; R6 is-hydrogen or fluorine; R7 is hydrogen or C1-6alkyl; R8 and R9 are each independently hydrogen, C1-6alkyl, -C(=NR10)NR14R15 or - C(O)R14 or R8 and R9 together form =CH(CRl4Rl5)faryl, =CH(CR14R15)fheterocyclyl, =CR14R15 or =C(R14)C(O)OR14, wherein the alkyl, aryl and heterocyclyl groups are optionally substituted by up to three groups independently selected from R16; R10 is -OR17, C1-6alkyl. -(CH2)garyl, -(CH2)gheterocyclyl or -(CH2)hO(CH2)iOR7, wherein each R10 group is optionally substituted by up to three groups independently selected from R16; R11is a heterocyclic group having the following structure: or R12 IS hydrogen or C1-6alkyl; R13 IS hydrogen or C1-4alkyl substituted by a group selected from optionally substituted phenyl, optionally substituted 5 or 6 membered heteroaryl and optionally substituted 9 to 10 membered fused bicycllc heteroaryl; R14 and R15 are each independently hydrogen or C1-6alkyl; R16 is halogen, cyano, nitro, trifluoromethyl, azido, -C(O)R21, -C(O)OR21, -OC(0)R21, - OC(O)OR21, -NR22C(0)R23, -C(O)NR22R23f -NR22R23, hydroxy, C1-6alkyl, -S(O)kC1_ galkyl, C1-6alkoxy, -(CH2)maryl or -(CH2)mneteroaryl. wherein the alkoxy group is optionally substituted by up to three groups independently selected from -NR14R15, halogen and -OR14, and the aryl and heteroaryl groups are optionally substituted by up to five groups independently selected from halogen, cyano, nitro, trifluoromethyl, azido, - C(O)R24 -C(O)OR24 -OC(O)OR24, -NR25C(O)R26. -C(O)NR25R26, -NR25R26, hydroxy, C1-6alkyl and C1-6alkoxy; WO 2004/101587 PCT/EP2004/005083 R17 is hydrogen, C1-6alkyl, C3-7cycloalkyl, C3-6alkenyl or a 5 or 6 membered heterocyclic group, wherein the alkyl, cyctoalkyl. alkenyl and heterocyclic groups are optionally substituted by up to three substituents independently selected from optionally substituted 5 or 6 membered heterocyclic group, optionally substituted 5 or 6 membered 5 heteroaryl, -OR27, -S(O)nR27, -NR27R28 -CONR27R28, halogen and cyano; R18 is hydrogen. -C(O)OR29, -C(O)NHR29 or-C(O)CH2N02; R19 IS hydrogen, C1-4alkyl optionally substituted by hydroxy or C1-4alkoxy, C3. 7cycloalkyl, or optionally substituted phenyl or benzyl; R20 is halogen, C1-4alkyl. C1-4thioalkyl, C1-4alkoxy, -NH2, -NH(C1-4alkyl) or -N(C1. 10 4alkyl)2; R21 is hydrogen, C1-10alkyl, -(CH2)paryl or-(CH2)pheteroaryl; R22 and R23 are each independently hydrogen, -OR14, C1-6alkyl. -(CH2)qaryl or - (CH2)qheterocyclyl; R24 IS hydrogen, C1-10alkyl, -(CH2)raryl or -(CH2)rheteroaryl; 15 R25 and R26 are each independently hydrogen, -OR14, C1-6alkyl, -(CH2)saryl or - (CH2)sheterocyclyl; R27 and R28 are each independently hydrogen, C1-4alkyl or C1-4alkoxyC1-4alkyl; R29 is hydrogen or C1-6alkyl optionally substituted by up to three groups independently selected from halogen, C1-4alkoxy, -OC(O)C1-6alkyl and -OC(O)OC1-6alkyl; 20 R30 is hydrogen, C1-4alkyl, C3-7cycloalkyl, optionally substituted phenyl or benzyl, acetyl or benzoyl; R31 is hydrogen or R20, or R31 and R19 are linked to form the bivalent radical -O(CH2)2- or-(CH2)t; X is-U(CH2)v-; 25 U is a divalent radical selected from -N(R30)-, -O-, -S(O)z, -N(R30)C(O)-, -C(O)N(R30)- and -N[C(O)R30]-; W is -C(R31)- or a nitrogen atom; d is an integer from 1 to 5; e is an integer from 2 to 4; 30 f, g, h, m, p, q, r and s are each independently integers from 0 to 4; I is an integer from 1 to 6; j, k, n and z are each independently integers from 0 to 2; tis2or3; v is an integer from 2 to 8; 35 and pharmaceutically acceptable derivatives thereof. The term "pharmaceutically acceptable" as used herein means a compound which is suitable for pharmaceutical use. Salts and solvates of compounds of the invention which are suitable for use in medicine are those wherein the counterion or associated solvent 40 is pharmaceutically acceptable. However, salts and solvates having non- pharmaceutically acceptable counterions or associated solvents are within the scope of WO 2004/101587 PCT/EP2004/005083 the present Invention, for example, for use as intermediates In the preparation of other compounds of the invention and their pharmaceutically acceptable salts and solvates. The term "pharmaceutically acceptable derivative" as used herein means any 5 pharmaceutically acceptable salt, solvate or prodrug, e.g. ester, of a compound of the invention, which upon administration to the recipient is capable of providing (directly or indirectly) a compound of the Invention, or an active metabolite or residue thereof. Such derivatives are recognizable to those skilled in the art, without undue experimentation. Nevertheless, reference is made to the teaching of Burger's Medicinal Chemistry and 10 Drug Discovery, 5th Edition, Vol 1: Principles and Practice, which is incorporated herein by reference to the extent of teaching such derivatives. Preferred pharmaceutically acceptable derivatives are salts, solvates, esters, carbamates and phosphate esters. Particularly preferred pharmaceutically acceptable derivatives are salts, solvates and esters. Most preferred pharmaceutically acceptable derivatives are salts and esters, in 15 particular salts. The compounds of the present Invention may be in the form of and/or may be administered as a pharmaceutically acceptable salt. For a review on suitable salts see Berge et a/., J. Pharm. Sci„ 1977,66,1-19. 20 Typically, a pharmaceutical acceptable salt may be readily prepared by using a desired acid or base as appropriate. The salt may precipitate from solution and be collected by filtration or may be recovered by evaporation of the solvent. For example, an aqueous solution of an acid such as hydrochloric acid may be added to an aqueous suspension of 25 a compound of formula (I) and the resulting mixture evaporated to dryness (lyophilised) to obtain the acid addition salt as a solid. Alternatively, a compound of formula (I) may be dissolved in a suitable solvent, for example an alcohol such as isopropanol, and the add may be added in the same solvent or another suitable solvent. The resulting acid addition salt may then be precipitated directly, or by addition of a less polar solvent such 30 as diisopropyl ether or hexane, and isolated by filtration. Suitable addition salts are formed from inorganic or organic acids which form non-toxic salts and examples are hydrochloride, hydrobromide, hydroiodide, sulphate, blsulphate, nitrate, phosphate, hydrogen phosphate, acetate, trifluoroacetafe, maleate, malate, 35 fumarate, lactate, tartrate, citrate, formate, gluconate, succinate, pyruvate, oxalate, oxaloacetate, trifluoroacetate, saccharate, benzoate, alkyl or aryl sulphonates (eg methanesulphonate, ethanesulphonate, benzenesulphonate or p-toluenesulphonate) and isethionate. Representative examples Include trifluoroacetate and formate salts, for example the bis or tris trifluoroacetate salts and the mono or diformate salts, in particular 40 the tris trifluoroacetate salt and the diformate salt A further representative example of a formate salt is the tris formate salt. WO 2004/101587 PCT/EP2004/005083 Pharmaceutically acceptable base salts Include ammonium salts, alkali metal salts such as those of sodium and potassium, alkaline earth metal salts such as those of calcium and magnesium and salts with organic bases, including salts of primary, secondary and tertiary amines, such as isopropylamine, diethylamine, ethanolamine, trimethylamine, 5 dicyclohexyl amine and N-methyl-D-glucamine. Compounds of the invention may have both a basic and an acidic centre may therefore be in the form of zwitterlons. 10 Those skilled in the art of organic chemistry will appreciate that many organic compounds can form complexes with solvents in which they are reacted or from which they are precipitated or crystallized. These complexes are known as "solvates". For example, a complex with water Is known as a "hydrate". Solvates of the compound of the invention are within the scope of the invention. The salts of the compound of formula 15 (I) may form solvates (e.g. hydrates) and the invention also includes all such solvates. The term "prodrug" as used herein means a compound which is converted within the body, e.g. by hydrolysis In the blood, into its active form that has medical effects. Pharmaceutically acceptable prodrugs are descrlbed in T. Higuchi and V. Stella, 20 "Prodrugs as Novel Delivery Systems", Vol. 14 of the ACS. Symposium Series, Edward B. Roche, ed., "Bloreversible Carriers in Drug Design", American Pharmaceutical Association and Pergamon Press, 1987, and in D. Fleisher, S. Ramon and H. Barbra "Improved oral drug delivery: solubility limitations overcome by the use of prodrugs", Advanced Drug Delivery Reviews (1996) 19(2) 115-130, each of which are incorporated 25 herein by reference. Prodrugs are any covalently bonded carriers that release a compound of structure (I) In vivo when such prodrug is administered to a patient. Prodrugs are generally prepared by modifying functional groups in a way such that the modification is cleaved, either by 30 routine manipulation or In vivo, yielding the parent compound. Prodrugs include, for example, compounds of this invention wherein hydroxy, amine or sulfhydryl groups are bonded to any group that, when administered to a patient, cleaves to form the hydroxy, amine or sulfhydryl groups. Thus, representative examples of prodrugs include (but are not limited to) acetate, formate and benzoate derivatives of alcohol, sulfhydryl and amine 35 functional groups of the compounds of structure (I). Further, in the case of a carboxylic acid (-COOH), esters may be employed, such as methyl esters, ethyl esters, and the like. Esters may be active in their own right and/or be hydrolysable under in vivo conditions in the human body. Suitable pharmaceutically acceptable In vivo hydrolysable ester groups include those which break down readily in the human body to leave the 40 parent acid or its salt. WO 2004/101587 PCT/EP2004/005083 References hereinafter to a compound according to the invention include both compounds of formula (I) and their pharmaceutically acceptable derivatives. With regard to stereoisomers, the compounds of structure (I) have more than one 5 asymmetric carbon atom. In the general formula (I) as drawn, the solid wedge shaped bond Indicates that the bond is above the plane of the paper. The broken bond indicates that the bond Is below the plane of the paper. It will be appreciated that the substituents on the macrollde may also have one or more 10 asymmetric carbon atoms. Thus, the compounds of structure (I) may occur as individual enantiomers or diastereomers. Ail such isomeric forms are included within the present invention, including mixtures thereof. Where a compound of the invention contains an alkenyl group, cis (Z) and trans (E) 15 isomerism may also occur. The present invention includes the Individual stereoisomers of the compound of the invention and, where appropriate, the individual tautomeric forms thereof, together with mixtures thereof. Separation of diastereoisomers or cis and trans isomers may be achieved by 20 conventional techniques, e.g. by fractional crystallisation, chromatography or H.P.L.C. A stereoisomerc mixture of the agent may also be prepared from a corresponding optically pure intermediate or by resolution, such as H.P.L.C., of the corresponding mixture using a suitable chiral support or by fractional crystallisation of the diastereoisomeric salts formed by reaction of the corresponding mixture with a suitable optically active acid or 25 base, as appropriate. The compounds of structure (I) may be in crystalline or amorphous form. Furthermore, some of the crystalline forms of the compounds of structure (I) may exist as polymorphs, which are included in the present invention. 30 Compounds wherein R2 represents a hydroxyl protecting group are in general intermediates for the preparation of other compounds of formula (I). When the group OR2 is a protected hydroxyl group this is conveniently an ether or an 35 acyloxy group. Examples of particularly suitable ether groups include those in which R2 is a trialkylsilyl (i.e. trimethylsilyl). When the group OR2 represents an acyloxy group, then examples of suitable groups R2 include acetyl or benzoyl. R6 is hydrogen or fluorine. However, it will be appreciated that when A is -C(0)NH- or - 40 CH2-N(R7)-, R6 is hydrogen. When R11 is a heterocyclic group having the following structure: WO 2004/101587 PCT/EP2004/005083 said heterocyclic is linked in the 5,6,7 or 8 position to the X group as above defined. In 5 one embodiment, the heterocyclic is linked In the 6 or 7 position. In another embodiment, the heterocyclic is linked in the 5 or 8 position. When present, the R20 group or groups may be attached at any position on the ring. In one embodiment, an R20 group is attached at the 6 position. 10 When R11 is a heterocyclic group having the following structure: R18 wherein W is -C(R31)- where R31 is R20 or R31 and R19 are linked to form the bivalent 15 radical -0(CH2)2- or -(CH2)t. said heterocyclic is linked in the (i), (ii) or (iii) position to the X group as above defined. In one embodiment, the heterocyclic is linked in the (I) position. In another embodiment, the heterocyclic is linked in the (ii) or (ill) position. When R11 is a heterocyclic group having the following structure: 20 said heterocyclic is linked in the 5,6 or 7 position to the X group as defined above. In one embodiment, the heterocyclic is linked in the 6 or 7 position. In another embodiment, the 25 heterocyclic is linked in the 5 position. When R11 is heterocyclic group having the following structure: WO 2004/101587 PCT/EP2004/00S083 said heterocyclic is linked in the 6, 7, 8 or 9 position to the X group as above defined. In one embodiment, the heterocyclic is linked in the 7 or 8 position. In another embodiment, 5 the heterocyclic is linked In the 6 or 9 position. When R11 Is a heterocyclic group having the following structure: 10 15 wherein W is -C(R31)- where R31 is R20 or R31 and R19 are linked to form the bivalent radical -O(CH2)2- or -(CH2)t. said heterocyclic is linked in the (i), (ii) or (ill) position to the X group as above defined. In one embodiment, the heterocyclic is linked in the (I) position. In another embodiment, the heterocyclic is linked in the (II) or (ill) position. When R11 is a heterocyclic group having the following structure: 20 said heterocyclic is linked in the 2,3 or 4 position to the X group as above defined. In one embodiment, the heterocyclic is linked In the 2 or 3 position. In another embodiment, the heterocyclic is linked in the 4 position. The term "alkyl" as used herein as a group or a part of a group refers to a straight or 25 branched hydrocarbon chain containing the specified number of carbon atoms. For example, C1-10alkyl means a straight or branched alkyl containing at least 1, and at most 10, carbon atoms. Examples of "alkyl" as used herein include, but are not limited to, methyl, ethyl, n-propyl, n-buiyl, n-pentyl, isobutyl, isopropyl, t-butyl, hexyl, heptyl, octyl, WO 2004/101587 PCT7EP2004/005083 nonyl and decyl. A C1-4alkyl group Is preferred, for example methyl, ethyl, n-propyl, Isopropyl, n-butyl, isobutyl ort-butyl. The term "C3-7cycloalkyl group as used herein refers to a non-aromatic monocyclic 5 hydrocarbon ring of 3 to 7 carbon atoms such as, for example, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl or cycloheptyl. The term "alkoxy" as used herein refers to a straight or branched chain alkoxy group containing the specified number of carbon atoms. For example, C1-6alkoxy means a 10 straight or branched alkoxy containing at least 1, and at most 6, carbon atoms. Examples of "alkoxy" as used herein include, but are not limited to, methoxy, ethoxy, propoxy, prop- 2-oxy, butoxy, but-2-oxy, 2-methylprop-l-oxy, 2-methylprop-2-oxy, pentoxy and hexyloxy. A C1-4alkoxy group is preferred, for example methoxy, ethoxy, propoxy, prop-2-oxy, butoxy, but-2-oxy or 2-methylprop-2-oxy. 15 The term "alkenyl" as used herein as a group or a part of a group refers to a straight or branched hydrocarbon chain containing the specified number of carbon atoms and containing at least one double bond. For example, the term "C2-6alkenyl" means a straight or branched alkenyl containing at least 2, and at most 6, carbon atoms and 20 containing at least one double bond. Similarly, the term "C3-6alkenyl* means a straight or branched alkenyi containing at least 3, and at most 6, carbon atoms and containing at least one double bond. Examples of "alkenyl" as used herein include, but are not limited to, ethenyl, 2-propenyl, 3-butenyl, 2-butenyl, 2-pentenyl, 3-pentenyl, 3-methyl-2-butenyl, 3-methylbut-2-enyl, 3-hexenyl and 1,1-dimethylbut-2-enyl. It will be appreciated that in 25 groups of the form -0-C2-6alkenyl, the double bond is preferably not adjacent to the oxygen. The term "alkynyl" as used herein as a group or a part of a group refers to a straight or branched hydrocarbon chain containing the specified number of carbon atoms and 30 containing at least one triple bond. For example, the term "C3-6alkenyl means a straight or branched alkynyl containing at least 3, and at most 6, carbon atoms containing at least one triple bond. Examples of "alkynyl" as used herein include, but are not limited to, propynyl, 1-butynyl, 2-butynyl, 1-pentynyl and 3-methyl-1-butynyl. 35 The term "aryl" as used herein refers to an aromatic carbocyclic moiety such as phenyl, biphenyl or naphthyl. The term "heteroaryl" as used herein, unless otherwise defined, refers to an aromatic heterocycle of 5 to 10 members, having at least one heteroatom selected from nitrogen, 40 oxygen and sulfur, and containing at least 1 carbon atom, including both mono and bicyciic ring systems. Examples of heteroaryl rings include, but are not limited to, furanyl, thiophenyl, pyrrolyl, pyrazolyi, imidazolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, WO 2004/101587 PCT/EP2004/005083 triazolyl, oxadiazolyl, tetrazolyl, thiadiazolyl, pyridyl, pyridazinyl, pyrazinyl, pyrimidlnyl, triazinyl, quinolinyl, isoquinolinyl, 1,2,3,4-tetrahydroisoqulnolinyl, benzofuranyl, benzimidazolyl,-benzothienyl, benzoxazolyl, 1,3-benzodioxazolyl, indolyl, benzothiazolyl, furylpyridine, oxazolopyridyl and benzothiophenyl. 5 The term "5 or 6 membered heteroaryl" as used herein as a group or a part of a group refers to a monocyclic 5 or 6 membered aromatic heterocycle containing at least one heteroatom independently selected from oxygen, nitrogen and sulfur. Examples include, but are not limited to, furanyl, thiophenyl, pyrrolyl, pyrazolyl, imldazolyl, oxazolyl, 10 isoxazolyl, thiazolyl, isothiazolyl, triazolyl, oxadiazolyl, tetrazolyl, pyridyl, pyridazinyl, pyrazinyl, pyrimidinyl and triazinyl. The term "9 to 10 membered fused bicyclic heteroaryl" as used herein as a group or a part of a group refers to quinolinyl, Isoquinolinyl, 1,2,3,4-tetrahydroisoquinolinyl, benzofuranyl, 15 benzimidazolyl, benzothienyl, benzoxazolyl, 1,3-benzodioxazolyl, indolyl, benzothiazolyl, furylpyridine, oxazolopyridyl or benzothiophenyl. The term "heterocyclyl" as used herein, unless otherwise defined, refers to a monocyclic or bicyclic three- to ten-membered saturated or non-aromatic, unsaturated hydrocarbon 20 ring containing at least one heteroatom selected from oxygen, nitrogen and sulfur. Preferably, the heterocyclyl ring has five or six ring atoms. Examples of heterocyclyl groups lnclude, but are not limited to, pyrrolidinyl, tetrahydrofuranyl, tetrahydrothiophenyl, imidazolidinyl, pyrazolidinyl, piperidyl, piperazinyl, morpholino, tetrahydropyranyl and thiomorpholino. 25 The term "5 or 6 membered heterocyclic group" as used herein as a group or part of a group refers to a monocyclic 5 or 6 membered saturated hydrocarbon ring containing at least one heteroatom independently selected from oxygen, nitrogen and sulfur. Examples of such heterocyclyl groups include, but are not limited to, pyrrolidinyl, tetrahydrofuranyl, 30 tetrahydrothiophenyl, imidazolidinyl, pyrazolidinyl, piperidyl, piperazinyl, morpholino, tetrahydropyranyl and thiomorpholino. The term "halogen" refers to a fluorine, chlorine, bromine or iodine atom. 35 The terms "optionally substituted phenyl", "optionally substituted phenyl or benzyl", "optionally substituted 5 or 6 membered heteroaryl", "optionally substituted 9 to 10 membered fused bicyclic heteroaryl" or "optionally substituted 5 or 6 membered heterocyclic group" as used herein refer to a group which is substituted by 1 to 3 groups selected from halogen, C^alkyl, C^alkoxy, hydroxy, nitro, cyano, amino, C1. 40 4alkylamino or diC1-4alkylamino, phenyl and 5 or 6 membered heteroaryl. WO 2004/101587 PCT/EP2004/005083 In one embodiment, A is -C(O)-, -C(O)NH-, -NHC(O)-, -N(R7)-CH2-, -CH2-N(R7)- or - CH(NR8R9)-. In another embodiment, A is -C(O)-, -C(O)NH-, -NHC(O)-, -CH2-N(R7)-, - CH(NR8R9)- or -C(=NR10) In another embodiment, A is -C(O)-f -NHC(O)-, -N(R7)-CH2- , -CH2-N(R7)-, -CH(NR8R9)- or -C(=NR10)-. In another embodiment, A is -C(O)-, - 5 C(O)NH-, -NHC(O)-, -CH2-N(R7)- or -CH(NR8R9)-. In a further embodiment, A is -C(O)-, -N(R7)-CH2- or -C(=NR10)-. Representative examples of A include -C(O)- and -N(R7)- CH2-. A further representative example of A is -C(=NR10)-. In particular, A is -C(O)-. 10 A representative example of R2 is hydrogen. Representative examples of R3 include hydrogen and C1-4alkyl, for example hydrogen and methyl. In particular, R3 is methyl. In one embodiment, R4 and R5 are hydroxy, R4 is C1-4alkoxy such as methoxy and R5 is 15 hydroxy, or R4 and R5 taken together with the intervening atoms form a cyclic group having the following structure: H3C wherein Y is a bivalent radical selected from -CH2-, -CH(CN)-, -O-, -N(R13)- and - CH(SR13)-. In another embodiment, R4 and R5 are hydroxy. In a further embodiment, 20 R4 is C1-4alkoxy such as methoxy and R5 is hydroxy. Alternatively, R4 and R5 taken together with the intervening atoms form a cyclic group having the following structure: wherein Y is a bivalent radical selected from -O- and -N(R13)-, 25 A representative example of R6 is hydrogen. A representative example of R7 is C1-6alkyl, for example C1-4alkyl, in particular methyl. 30 A representative example of R10 is -OR17. In one embodiment, R11 includes heterocyclic groups having the following structure: WO 2004/101587 PCT/EP2004/005083 wherein the heterocyclic is linked in the 6 or 7 position to the X group as above defined, and heterocyclic groups having the following structure: wherein W is -C(R31)- where R31 and R19 are linked to form the bivalent radical - O(CH2)2- or-(CH2)t. in particular-(CH2)t, and said heterocyclic is linked in the (i), (ii) or 10 (iii) position, in particular the (ii) position, to the X group as above defined. Representative examples of R11 include heterocyclic groups having the following structure: 15 wherein the heterocyclic is linked in the 6 or 7 position to the X group as above defined. Further representative examples of R11 include heterocyclic groups having the following 20 structure: WO 2004/101587 PCT/EP2004/005083 wherein W is -C(R31). where R31 and R19 are linked to form the bivalent radical - O(CH2)2- or-(CH2)t-, in particular-(CH2)t, and said heterocyclic is linked in the (i), (ii) or (iii) position, in particular the (ii) position,-to the X group as above defined. 5 In one embodiment, R13 is hydrogen or C1-4alkyl substituted by a group selected from optionally substituted phenyl, optionally substituted 5 or 6 membered heteroaryl and optionally substituted 9 to 10 membered fused blcycllc heteroaryl. In another embodiment, R13 is hydrogen or C1-4alkyl. A representative example of R13 is hydrogen. A further representative example of R13 is methyl. 10 Representative examples of R17 include hydrogen and C1-6alkyl, for example C1-4alkyl, In particular methyl, optionally substituted by -OR27. In one embodiment, R18 is hydrogen, -C(O)OR29, -C(O)NHR29 or -C(O)CH2N02- In 15 another embodiment, R18 is -C(O)OR29, -C(O)NHR29 or -C(O)CH2NO2. In another embodiment, R18 is -C(O)OR29. In a further embodiment, R18 is -C(O)OR29, wherein R29 is hydrogen, C1-6alkyl optionally substituted by up to three groups Independently selected from C1-4alkoxy, -OC(O)C1-6alkyl, -C(O)NR32R33 and NR32 R33 -(CH2)wC3- 7cycloalkyl, C3-6alkenyl or C3-6alkynyl. A representative example of R18 is -C(O)OR29, 20 wherein R29 is hydrogen or C1-4alkyl, for example hydrogen or methyl. Further representative examples of R18 Include -C(O)OR29, wherein R29 is hydrogen; C1-6alkyl, for example C1-4alkyl such as methyl, ethyl, isopropyl, isobutyl or n-butyl, optionally substituted by up to three groups independently selected from C1-4alkoxy such as methoxy, -OC(O)C1-6alkyl such as -OC(O)t-butyl, -C(O)NR32R33 and -NR32R33; - 25 (CH2)wC3-7cydoalkyl, for example -(CH2)wc3-6cyioalkyl such as -(CH2)wcyclopropyl; C3-6alkenyl, for example C3-4alkenyl such as 2-propenyl or 3-butenyl; or C3-6alkynyl, for example C3-4alkynyl such as 2-butynyl. In particular, R29 is hydrogen. In one embodiment, R19 is C1-4alkyl, for example methyl or ethyl, optionally substituted 30 by C1-4alkoxy, for example methoxy, or R19 is C3-7cycloalkyl, for example C3. 6cycloalkyl such as cyclopropyl. A representative example of R19 is C1-4alkyl, in particular ethyl. A representative example of R20 is halogen, in particular fluorine. 35 A representative example of R27 is C1-4alkoxyC1-4alkyl. In one embodiment, R29 is hydrogen or C1-6alkyl optionally substituted by up to three groups independently selected from halogen, C1-4alkoxy, -OC(O)C1-6alkyl and - 40 OC(O)oC1-6alkyl. In another embodiment, R29 is hydrogen, C1-6alkyl optionally substituted by up to three groups independently selected from halogen, C1-4alkoxy, - OC(O)C1-6alkyl, -OC(O)OC1-6alkyl, -C(O)NR32R33 and -NR32R33, -(CH2)WC3- WO 2004/101587 PCT/EP2004/005083 7cycloalkyl, C3-6alkenyl or C3-6alkynyl. In a further embodiment, R29 is hydrogen, C1- 6alkyl optionally substituted by up to three groups independently selected from C1. 4alkoxy, -OC(O)C1-6alkyl, -C(O)NR32R33 and -NR32R33; -(CH2)wC3-7cycloalkyl; C3.' 6alkenyl; or C3-6alkynyl. Representative examples of R29 Include hydrogen; C1-6alkyl, 5 for example C1-4alkyl such as methyl, ethyl, isopropyl, 2-methylpropyl or n-butyl, optionally substituted by up to three groups independently selected from C1-4alkoxy such as methoxy, -OC(O)C1-6alkyl sucn as -OC(O)t-butyl, -C(O)NR32R33 and -NR32R33; - (CH2)wC3-7cycloalkyl, for example -(CH2)wC3-6cycloalkyl such as -(CH2)wcyclopropyl; C3-6alkenyl, for example C3-4alkenyl such as 2-propenyl or 3-butenyl; 0and C3-6alkynyl, 10 for example C3-4alkynyl such as 2-butynyl. In particular, R29 is hydrogen. In one embodiment, R30 is hydrogen or C1-4alkyl. A representative example of R30 is hydrogen. 15 In one embodiment, R31 is hydrogen or R31 and R19 are linked to form the bivalent radical -(CH2)r- A representative example of R31 is hydrogen. In one embodiment, R32 and R33 are each independently hydrogen or C1-6alkyl optionally substituted by-C(O)OC1-6alkyl, or 20 R32 and R33, together with the nitrogen atom to which they are bound, form a 5 or 6 membered heterocyclic group optionally containing one additional heteroatom selected from oxygen, nitrogen and sulfur. In another embodiment, R32 and R33 are each independently hydrogen or C1-6alkyl, for 25 example C1-4alkyl such as methyl, optionally substituted by -C(O)OC1-6alkyl, for example -C(O)OC1-4alkyl such as -C(O)Oethyl. In a further embodiment, R32 and R33, together with the nitrogen atom to which they are bound, form a 6 membered heterocyclic group optionally containing one additional oxygen 30 atom. In one embodiment, X is -U(CH2)v- wherein U is a divalent radical selected from -N(R39)-, -O- and -S(O)z-. In a further embodiment, X is -U(CH2)v- wherein U is a divalent radical selected from -N(R30)- and -0-. A representative example of X is -U(CH2)v wherein U is 35 the divalent radical -N(R30)-. A further representative example of X Is -U(CH2)v wherein U is the divalent radical -O-. A representative example of W is -C(R31)-. 40 Representative examples of Y Include -O- and -N(R13)-. WO 2004/101587 PCT/EP2004/005083 5 10 In one embodiment, d is an integer from 2 to 5. A representative example of d is 1 to 3, for example 2. A further representative example of d is 5. A representative example of w is 1. Representative examples of t are 2 and 3. In particular, t Is 3. in one embodiment, v is an integer of from 2 to 8. A representative example of y Is 2 to 4, for example 3. Representative examples of j include 0 and 1. In particular, j is 0. It is to be understood that the present invention covers all combinations of particular and preferred groups described hereinabove. It is also to be understood that the present 15 invention encompasses compounds of formula (I) in which a particular group or parameter, for example R7 R14, R15 R16, R20, R21, R22 R23 .R24 R25 R26 R27 R28, R32, R33, k, m, n, p, q, r and s may occur more than once. In such compounds it will be appreciated that each group or parameter is independently selected from the values listed. 20 In one embodiment, when A is -C(O)-, d is 2, X is -NH(CH2)3- and R11 is a heterocyclic group of the following formula: 25 wherein the heterocyclic Is linked in the 6 or 7 position to the X group, j is 0, R18 is carboxy and R19 is ethyl. In a further embodiment, when A Is -C(O)-, d Is 2, X is -NH(CH2)3- and R11 is a heterocyclic group of the following formula: 30 WO 2004/101587 PCT/EP2004/005083 wherein W is -C(R31)- where R31 and R19 are linked to form the bivalent radical -(CH2)t, said heterocyclic is linked in the (ii) or (iii) position to the X group, J is 0 and R18 is carboxy. 5 Particularly preferred compounds of the invention are: 4"O-{3-[3-(3-carboxy-1,4-dihydro-1-ethyl-4-oxo-6-quinollnyl)propylamino]proplony}-6-O- methylerythrornycin A; 4"-O-{3-[3-(3-carboxy-1,4-dlhydro-1 -ethyl-4-oxo-6-quinolinyl) propylamlno]propionyl}-azlthromycin-11,12-carbonate; 10 4"-0-{3-[3-(3-carboxy-1,4-dihydro-1-ethyl-4-oxo-6-quinolinyl) propylamlno]propionyl}-6-0-rnethyl-11 -desoxy-11 -(R)-amino-erythromycin A 11,12- carbamate; and pharmaceutically acceptable derivatives thereof. 15 Further particularly preferred compounds of the invention are: 4"-0-[3-[4-(2-carboxy-6,7-dihydro-1HI5H-pyrido[3,2,1-ij]-1-oxo-9- quinolinyl) propylamino]propionyl]-6-0-methyl erythromycin A; 4"-0-[3-{4-(3-carboxy-1-ethyl-1,4-dlhydro-4-oxo-6-quinollnyl)propyIamino]propionyl]-(9E)- 0-({[2-(methyloxy)ethyl]oxy}methanoximino erythromycin A; 20 4"-0-[3-[4-(3-carboxy-1 -ethyl-1,4rdlhydro-4-oxo-6-quinolinyl)propylamino]propionyl]-(9E)- O-hydroxImino erythromycin A; 4"-0-[3-[4-(2-carboxy-6,7-dihydro-1H,5H-pyrido[3,2,1-ij]-1-oxo-9- qulnollnyl) propylamino]propionyl}-(9E)-O-hydroximino erythromycin A; and pharmaceutically acceptable derivatives thereof. 25 Additional particularly preferred compounds of the invention are: 4"-0-{6-[3-(3-carboxy-1-ethyl-4-oxo-1,4-dihydro-quinolln-6-yl)-propoxy]-h6xanoyl}- azithromycin; 4"-0-{6-[3-(3-carboxy-1-ethyl-4-oxo-1,4-dihydro-quinolin-6-yl)-propoxy]-hexanoyl}- 30 clarithromycin; and pharmaceutically acceptable derivatives thereof. Compounds according to the invention also exhibit a broad spectrum of antimicrobial activity, in particular antibacterial activity, against a wide range of clinical pathogenic 35 microorganisms. Using a standard microtiter broth serial dilution test, compounds of the invention have been found to exhibit useful levels of activity against a wide range of pathogenic microorganisims. In particular, the compounds of the invention may be active against strains of Staphylococcus aureus, Streptopococcus pneumoniae, Moraxella catarrhalis, Streptococcus pyogenes, Haemophilus influenzae, Enterococcus faecalis, 40 Chlamydia pneumoniae, Mycoplasma pneumoniae and Legionella pneumophila. The compounds of the invention may also be active against resistant strains, for example erythromycin resistant strains. In particular, the compounds of the invention may be WO 2004/101587 PCT/EP2004/005083 active against erythromycin resistant strains of Streptococcus pneumoniae, Streptococcus pyogenes and Staphylococcus aureus. The compounds of the invention may therefore be used for treating a variety of diseases 5 caused by pathogenic microorganisms, in particular bacteria, in human beings and animals. It will be appreciated that reference to treatment includes acute treatment or prophylaxis as well as the alleviation of established symptoms. Thus, according to another aspect of the present invention we provide a compound of 10 formula (I) or a pharmaceutically acceptable derivative thereof for use in therapy. According to a further aspect of the invention we provide a compound of formula (I) or a pharmaceutically acceptable derivative thereof for use In the therapy or prophylaxis of systemic or topical microbial infections in a human or animal subject. 15 According to a further aspect of the invention we provide the use of a compound of formula (I) or a pharmaceutically acceptable derivative thereof in the manufacture of a medicament for use in the treatment or prophylaxis of systemic or toplcal microbial infections in a human or animal body. 20 According to a yet further aspect of the invention we provide a method of treatment of the human or non-human animal body to combat microbial infections comprising administration to a body in need of such treatment of an effective amount of a compound of formula (I) or a pharmaceutically acceptable derivative thereof. 25 While it is possible that, for use in therapy, a compound of the invention may be administered as the raw chemical it is preferable to present the active ingredient as a pharmaceutical formulation eg when the agent is in admixture with a suitable pharmaceutical excipient, diluent or carrier selected with regard to the intended route of 30 administration and standard pharmaceutical practice. Accordingly, in one aspect, the present invention provides a pharmaceutical composition or formulation comprising at least one compound of the invention or a pharmaceutically acceptable derivative thereof in association with a pharmaceutically acceptable 35 excipient, diluent and/or carrier. The excipient, diluent and/or carrier must be "acceptable" in the sense of being compatible with the other ingredients of the formulation and not deleterious to the recipient thereof. In another aspect, the invention provides a pharmaceutical composition comprising, as 40 active ingredient, at least one compound of the invention or a pharmaceutically acceptable derivative thereof in association with a pharmaceutically acceptable excipient, diluent and/or carrier for use in therapy, and in particular, in the treatment of WO 2004/101587 PCT/EP2004/005083 human or animal subjects suffering from a condition susceptible to amelioration by an antimicrobial compound. In another aspect, the invention provides a pharmaceutical composition comprising a 5 therapeutically effective amount of the compounds of the present invention and a pharmaceutically acceptable excipient, diluent and/or carrier (including combinations thereof). There is further provided by the present invention a process of preparing a 10 pharmaceutical composition, which process comprises mixing at least one compound of the invention or a pharmaceutically acceptable derivative thereof, together with a pharmaceutically acceptable excipient, diluent and/or carrier. The compounds of the Invention may be formulated for administration in any convenient 15 way for use in human or veterinary medicine and the invention therefore includes within its scope pharmaceutical compositions comprising a compound of the invention adapted for use in human or veterinary medicine. Such compositions may be presented for use In a conventional manner with the aid of one or more suitable excipients, diluents and/or carriers. Acceptable excipients, diluents and carriers for therapetic use are well known 20 in the pharmaceutical art, and are described, for example, in Remington's Pharmaceutical Sciences, Mack Publishing Co. (A. R. Gennaro edit. 1985). The choice of pharmaceutical excipient, diluent and/or carrier can be selected with regard to the intended route of administration and standard pharmaceutical practice. The pharmaceutical compositions may comprise as ~ or in addition to - the excipient, diluent 25 and/or carrier any suitable binder(s), lubricant(s), suspending agent(s), coating agent(s), solubilising agent(s). Preservatives, stabilisers, dyes and even flavouring agents may be provided in the pharmaceutical composition. Examples of preservatives include sodium benzoate, sorbic 30 acid and esters of p-hydroxybenzolc acid. Antioxidants and suspending agents may be also used. For some embodiments, the agents of the present invention may also be used in combination with a cyclodextrin. Cyclodextrins are known to form inclusion and non- 35 inclusion complexes with drug molecules. Formation of a drug-cyclodextrin complex may modify the solubility, dissolution rate, bioavailability and/or stability property of a drug molecule. Drug-cyclodextrin complexes are generally useful for most dosage forms and administration routes. As an alternative to direct complexation with the drug the cyclodextrin may be used as an auxiliary additive, e. g. as a carrier, diluent or solubiliser. 40 Alpha-, beta- and gamma-cyclodextrins are most commonly used and suitable examples are described in WO 91/11172, WO 94/02518 and WO 98/55148. WO 2004/101587 PCT/EP2004/005083 The compounds of the invention may be milled using known milling procedures such as wet milling to obtain a particle size appropriate for tablet formation and for other formulation types. Finely divided (nanoparticulate) preparations of the compounds of the invention may be prepared by processes known in the art, for example see International 5 Patent Application No. WO 02/00196 (SmithKline Beecham). The routes for administration (delivery) include, but are not limited to, one or more of: oral (e. g. as a tablet, capsule, or as an ingestable solution), topical, mucosal (e. g. as a nasal spray or aerosol for inhalation), nasal, parenteral (e. g. by an injectable form), 10 gastrointestinal, intraspinal, intraperitoneal, intramuscular, intravenous, intrauterine, intraocular, intradermal, intracranial, intratracheal, intravaglnal, intracerebroventricular, intracerebral, subcutaneous, ophthalmic (including intravitreal or intracameral), transdermal, rectal, buccal, epidural and sublingual. 15 There may be different composition/formulation requirements depending on the different delivery systems. By way of example, the pharmaceutical composition of the present invention may be formulated to be delivered using a mini-pump or by a mucosal route, for example, as a nasal spray or aerosol for inhalation or ingestable solution, or parenterally in which the composition is formulated by an injectable form, for delivery, by, 20 for example, an intravenous, intramuscular or subcutaneous route. Alternatively, the formulation may be designed to be delivered by both routes. Where the agent is to be delivered mucosaJly through the gastrointestinal mucosa, it should be able to remain stable during transit though the gastrointestinal tract; for 25 example, it should be resistant to proteolytic degradation, stable at acid pH and resistant to the detergent effects of bile. Where appropriate, the pharmaceutical compositions can be administered by inhalation, in the form of a suppository or pessary, topically in the form of a lotion, solution, cream, 30 ointment or dusting powder, by use of a skin patch, orally in the form of tablets containing excipients such as starch or lactose, or in capsules or ovules either alone or in admixture with excipients, or in the form of elixirs, solutions or suspensions containing flavouring or colouring agents, Or they can be injected parenterally, for example intravenously, intramuscularly or subcutaneously. For parenteral administration, the 35 compositions may be best used in the form of a sterile aqueous solution which may contain other substances, for example enough salts or monosaccharides to make the solution isotonic with blood. For buccal or sublingual administration the compositions may be administered in the form of tablets or lozenges which can be formulated in a conventional manner. 40 WO 2004/101587 PCT/EP2004/005083 It is to be understood that not all of the compounds need be administered by the same route. Likewise, if the composition comprises more than one active component, then those components may be administered by different routes. 5 The compositions of the invention include those in a form especially formulated for parenteral, oral, buccal, rectal, topical, implant, ophthalmic, nasal or genito-urinary use. For some applications, the agents of the present invention are delivered systemically (such as orally, buccally, sublingually), more preferably orally. Hence, preferably the agent is in a form that is suitable for oral delivery. 10 If the compound of the present invention is administered parenterally, then examples of such administration include one or more of: intravenously, intraarterially, intraperitoneally, intrathecally, intraventricularly, intraurethrally, intrasternally, intracranfally, intramuscularly or subcutaneously administering the agent; and/or by 15 using infusion techniques. For parenteral administration, the compound is best used in the form of a sterile aqueous solution which may contain other substances, for example, enough salts or glucose to make the solution isotonic with blood. The aqueous solutions should be 20 suitably buffered (preferably to a pH of from 3 to 9), if necessary. The preparation of suitable parenteral formulations under sterile conditions is readily accomplished by standard pharmaceutical techniques well-known to those skilled in the art. The compounds according to the invention may be formulated for use in human or 25 veterinary medicine by injection (e.g. by intravenous bolus injection or infusion or via Intramuscular, subcutaneous or intrathecal routes) and may be presented in unit dose form, in ampoules, or other unit-dose containers, or In multi-dose containers, if necessary with an added preservative. The compositions for injection may be in the form of suspensions, solutions, or emulsions, in oily or aqueous vehicles, and may contain 30 formulatory agents such as suspending, stabilising, solubilislng and/or dispersing agents. Alternatively the active ingredient may be in sterile powder form for reconstitutlon with a suitable vehicle, e.g. sterile, pyrogen-free water, before use. The compounds of the invention can be administered (e. g. orally or topically) in the form 35 of tablets, capsules, ovules, elixirs, solutions or suspensions, which may contain flavouring or colouring agents, for Immediate-, delayed-, modified-, sustained-, pulsed- or controlled-release applications. The compounds of the invention may also be presented for human or veterinary use in a 40 form suitable for oral or buccal administration, for example In the form of solutions, gels, syrups, mouth washes or suspensions, or a dry powder for constitution with water or other suitable vehicle before use, optionally with flavouring and colouring agents. Solid WO 2004/101587 PCT/EP2004/005083 compositions such as tablets, capsules, lozenges, pastilles, pills, boluses, powder, pastes, granules, bullets or premix preparations may also be used. Solid and liquid compositions for oral use may be -prepared according to methods well known in the art. Such compositions may also contain one or more pharmaceutically acceptable carriers and 5 excipients which may be in solid or liquid form. The tablets may contain excipients such as mlcrocrystalline cellulose, lactose, sodium citrate, calcium carbonate, dibasic calcium phosphate and glycine, disintegrants such as starch (preferably com, .potato or tapioca starch), sodium starch glycoilate, 10 croscarmellose sodium and certain complex silicates, and granulation binders such as polyvinylpyrrolidone, hydroxypropylmethylcellulose (HPMC), hydroxypropylcellulose (HPC), sucrose, gelatin and acacia. Additionally, lubricating agents such as magnesium stearate, stearic acid, glyceryl 15 behenate and talc may be included. Solid compositions of a similar type may also be employed as fillers in gelatin capsules. Preferred excipients in this regard include lactose, starch, a cellulose, milk sugar or high molecular weight polyethylene glycols. For aqueous suspensions and/or elixirs, the agent may be combined with various sweetening or flavouring agents, colouring matter or dyes, with emulsifying and/or suspending agents and with diluents such as water, ethanoi, propylene glycol and glycerin, and combinations thereof. The compounds of the Invention may also be administered orally in veterinary medicine in 25 the form of a liquid drench such as a solution, suspension or dispersion of the active ingredient together with a pharmaceutically acceptable carrier or exciplent. The compounds of the invention may also, for example, be formulated as suppositories e.g. containing conventional suppository bases for use in human or veterinary medicine or 30 as pessaries e.g. containing conventional pessary bases. The compounds according to the invention may be formulated for topical administration, for use in human and veterinary medicine, in the form of ointments, creams, gels, hydrogels, lotions, solutions, shampoos, powders (including spray or dusting powders), 35 pessaries, tampons, sprays, dips, aerosols, drops (e.g. eye ear or nose drops) or pour- ons. For application topically to the skin, the agent of the present invention can be formulated as a suitable ointment containing the active compound suspended or dissolved in, for 40 example, a mixture with one or more of the following: mineral oil, liquid petrolatum, white petrolatum, propylene glycol, polyoxyethylene polyoxypropylene compound, emulsifying wax and water. WO 2004/101587 PCT/EP2004/005083 Alternatively, it can be formulated as a suitable lotion or cream, suspended or dissolved in, for example, a mixture of one or more of the following: mineral oil, sorbitan monostearate, a polyethylene glycol, liquid paraffin, polysorbate 60, cetyl esters wax, 5 cetearyl alcohol, 2-octyldodecanol, benzyl alcohol and water. The compounds may also be dermally or transdermally administered, for example, by use of a skin patch. 10 For ophthalmic use, the compounds can be formulated as micronised suspensions in isotonic, pH adjusted, sterile saline, or, preferably, as solutions in isotonic, pH adjusted, sterile saline, optionally in combination with a preservative such as a benzylalkonlum chloride. Alternatively, they may be formulated in an ointment such as petrolatum. 15 As indicated, the compound of the present invention can be administered intranasal^ or by inhalation and is conveniently delivered in the form of a dry powder inhaler or an aerosol spray presentation from a pressurised container, pump, spray or nebuliser with the use of a suitable propellant, e. g. dichlorodifluoromethane, trichlorofiuoromethane,' dichlorotetrafluoroethane, a hydrofluoroalkane such as 1,1,1,2-tetrafluoroethane (HFA 20 134AT"") or 1,1,1,2,3,3,3-heptafluoropropane (HFA 227EA), carbon dioxide or other suitable gas. In the case of a pressurised aerosol, the dosage unit may be determined by providing a valve to deliver a metered amount. The pressurised container, pump, spray or nebuliser may contain a solution or suspension of the active compound, e. g. using a mixture of ethanol and the propellant as the solvent, which may additionally 25 contain a lubricant, e. g. sorbitan trioleate. Capsules and cartridges (made, for example, from gelatin) for use in an inhaler or insufflator may be formulated to contain a powder mix of the compound and a suitable powder base such as lactose or starch. 30 For topical administration by inhalation the compounds according to the invention may be delivered for use in human or veterinary medicine via a nebuliser. The compounds of the invention may also be used in combination with other therapeutic 35 agents. The invention thus provides, in a further aspect, a combination comprising a compound of the invention or a pharmaceutlcally acceptable derivative thereof together with a further therapeutic agent. When a compound of the invention or a pharmaceutlcally acceptable derivative thereof 40 is used in combination with a second therapeutic agent active against the same disease state the dose of each compound may differ from that when the compound is used alone. Appropriate doses will be readily appreciated by those skilled in the art It will be WO 2004/101587 PCT/EP2004/005083 appreciated that the amount of a compound of the invention required for use In treatment will vary with the nature of the condition being treated and the age and the condition of the patient and will be ultimately at the discretion of the attendant physician or veterinarian. The compounds of the present invention may for example be used for 5 topical administration with other active Ingredients such as corticosteroids or antifungals as appropriate. The combinations referred to above may conveniently be presented for use in the form of a pharmaceutical formulation and thus pharmaceutical formulations comprising a 10 combination as defined above together with a pharmaceutically acceptable carrier or excipient comprise a further aspect of the invention. The individual components of such combinations may be administered either sequentially or simultaneously in separate or combined pharmaceutical formulations by any convenient route. 15 When administration is sequential, either the compound of the invention or the second therapeutic agent may be administered first. When administration is simultaneous, the combination may be administered either in the same or different pharmaceutical composition. 20 When combined in the same formulation it will be appreciated that the two compounds must be stable and compatible with each other and the other components of the formulation. When formulated separately they may be provided in any convenient formulation, conveniently in such manner as are known for such compounds in the art. 25 The compositions may contain from 0.01-99% of the active material. For topical administration, for example, the composition will generally contain from 0.01-10%, more preferably 0.01-1% of the active material. Typically, a physician will determine the actual dosage which will be most suitable for an 30 individual subject. The specific dose level and frequency of dosage for any particular individual may be varied and will depend upon a variety of factors including the activity of the specific compound employed, the metabolic stability and length of action of that compound, the age, body weight, general health, sex, diet, mode and time of administration, rate of excretion, drug combination, the severity of the particular condition, 35 and the Individual undergoing therapy. For oral and parenteral administration to humans, the daily dosage level of the agent may be in single or divided doses. 40 For systemic administration the dally dose as employed for adult human treatment it will range from 2-100mg/kg body weight, preferably 5-60mg/kg body weight, which may be administered in 1 to 4 daily doses, for example, depending on the route of administration WO 2004/101587 PCT/EP2004/005083 and the condition of the patient When the composition comprises dosage units, each unit will preferably contain 200mg to 1g of active ingredient The duration of treatment will be dictated by the rate of response rather than by arbitrary numbers of days. 5 Compounds of general formula (I) and salts thereof may be prepared by the general methods outlined hereinafter, said methods constituting a further aspect of the invention. In the following description, the groups R1 to R33, A, X, Y, U, W, d, e, f, g, h, i, j, k, m, n, p, q, r, s, t, v, w and z have the meaning defined for the compounds of formula (I) unless otherwise stated. 10 The group XaR11a is XR11 as defined for formula (I) or a group convertible to XR11. Conversion of a group XaR11a to a XR11 group typically arises If a protecting group is needed during the reactions described below. A comprehensive discussion of the ways in which such groups may be protected and methods for cleaving the resulting protected 15 derivatives is given by for example T.W. Greene and P.G.M Wuts in Protective Groups in Organic Synthesis 2nd ed., John Wiley & Son, Inc 1991 and by P.J. Kocienski in Protecting Groups, Georg Thieme Verlag 1994 which are incorporated herein by reference. Examples of suitable amino protecting groups include acyl type protecting groups (e.g. formyl, trifluoroacetyl and acetyl), aromatic urethane type protecting groups (e.g. 20 benzyloxycarbonyl (Cbz) and substituted Cbz, and 9-fluorenylmethoxycarbonyl (Fmoc)), aliphatic urethane protecting groups (e.g. t-butyloxycarbonyl (Boc), isopropyloxycarbonyl and cyclohexyloxycarbonyl) and alkyl type protecting groups (e.g. benzyl, trityl and chlorotrityl). Examples of suitable oxygen protecting groups may Include for example alkyl siiyl groups, such as trimethylsilyl or tert-butyldimethylsilyl; alkyl ethers such as 25 tetrahydropyranyl or tert-butyl; or esters such as acetate. Hydroxy groups may be protected by reaction of for example acetic anhydride, benzoic anhydride or a trialkylsflyl chloride in an aprotlc solvent. Examples of aprotic solvents are dichloromethane, N,N- dimethylformamide, dimethylsulfoxide, tetrahydrofuran and the like. 30 Compounds of formula (I) may be prepared by reaction of a 4" hydroxy compound of formula (II) wherein R2 is a hydroxy protecting group with a suitable activated and protected derivative of the carboxyiic acid (ill), followed where necessary by subsequent removal of the hydroxy! protecting group R2 and conversion of the XaR11a group to XR11. WO 2004/101587 PCT/EP2004/005083 HOC(O)(CH2)dXaR11a (II) (III) Suitable activated derivatives of the carboxyl group include the corresponding acyl halide, 5 mixed anhydride or activated ester such as a thioester. The reaction is preferably carried out in a suitable aprotic solvent such as a halohydrocarbon (e.g. dichloromethane) or N,N- dimethylformamide optionally in the presence of a tertiary organic base such as dimethylarhinopyridine or triethylamine or in the presence of Inorganic base (eg sodium hydroxide) and at a temperature within the range of 0° to 120°C. The compounds of 10 formula (II) and (III) may also be reacted in the presence of a carbodiimide such as dicyclohexylcarbodiimide (DCC). In a further embodiment of the invention, compounds of formula (I) wherein U Is a group selected from -N(R30)- and -S-, may be prepared by reaction of compounds of formula 15 (IV), wherein d is an integer from 1 to 5 and L is a suitable leaving group, with XaR11a (V) in 20 which U Is a group selected from -N(R30)- and -S-. The reaction Is preferably carried out in a solvent such as a halohydrocarbon (e.g. dichloromethane), an ether (e.g. WO 2004/101587 PCT7EP2004/005083 tetrahydrofuran or dimethoxyethane), acetonitrile or ethyl acetate and the like, dimethylsulfoxide, N,N-dimethylformamlde or 1-methyl-pyrrolldone and in the presence of a base, followed, .if desired, by removal of the hydroxyl protecting group R2 and conversion of the XaR11a group to XR11. Examples of the bases which may be used include organic bases such as diisopropylethylamine, triethylamine and 1,8- diazabicyclo[5.4.0]undec-7-ene, and inorganic bases such as potassium hydroxide, cesium hydroxide, tetraalkylammonium hydroxide, sodium hydride, potassium hydride and the like. Suitable leaving groups for this reaction include halide (e.g. chloride, bromide or iodide) or a sulfonyloxy group (e.g. tosyloxy or methanesulfonyloxy). Compounds of formula (IV) may be prepared by reaction of a compound of formula (II), wherein R2 is a hydroxyl protecting group, with a suitable activated derivative of the carboxylic acid HOC(0)(CH2)dL (VI), wherein L is a suitable leaving group as above defined. Suitable activated derivatives of the carboxyl group are those defined above for carboxylic acid (III). The reaction is carried out using the conditions described above for the reaction of a compound of formula (II) with carboxylic acid (III). In a preferred embodiment of the invention, compounds of formula (I) wherein d is 2 and U is a group selected from -N(R30)- and -S-, may be prepared by Michael reaction of a compound of formula (VII), wherein R2 is optionally a hydroxyl protecting group with a compound of formula XaR11a (V). The reaction is suitably carried out in a solvent such as dimethylsulfoxide, N,N-dimethylformamide, 1-methyl-pyrrolldone, a halohydrocarbon (e.g. dichloromethane), an ether (e.g. tetrahydrofuran or dimethoxyethane), acetonitrile or alcohol (e.g methanol or isopropanol) and the like, and in the presence of a base, followed, if desired, by removal of hydroxyl protecting group R2 and conversion of the XaR11a group to XR11. WO 2004/101587 PCT/EP2004/005083 Compounds of formula (I) may be converted into other compounds of formula (I). Thus compounds of formula (I) wherein U Is -S(O)r and z is 1 or 2 may be prepared by oxidation of the corresponding compound of formula (I) wherein z is 0. The oxidation is preferably carried out using a peracid, e.g. peroxybenzoic acid, followed by treatment with 5 a phosphine, such as triphenylphosphine. The reaction is suitably carried out in an organic solvent such as methylene chloride. Compounds of formula (I) wherein U is -N(R30)- and R30 IS c1-4alkyl can be prepared from compounds wherein R30 Is hydrogen by reductive alkylation. 10 Compounds of formula (II) wherein A is -C(O)NH- or -NHC(O)-, R4 or R5 are hydroxy, R3 is hydrogen and R6 is hydrogen are known compounds or they may be prepared by analogous methods to those known in the art. Thus they can be prepared according to the procedures described in EP 507595 and EP 503932. 15 Compounds of formula (II), wherein A is -C(O)NH- or -NHC(O)-, R4 or R5 are hydroxy and R3 is C1-4alkyl or C3-6alkenyl optionally substituted by 9 to 10 membered fused bicyclic heteroaryl and R6 is hydrogen are known compounds or they may be prepared by analogous methods to those known in the art. Thus they can be prepared according to the procedures described in WO 9951616 and WO 0063223. 20 Compounds of formula (II), wherein A is -C(O)NH-, R4 and R5 taken together with the intervening atoms form a cyclic group having the following structure: R3 Is C1-4alkyl, or C3-6alkenyl optionally substituted by 9 to 10 membered fused bicyclic 25 heteroaryl and R6 is hydrogen are known compounds or they may be prepared by analogous methods to those known in the art. Thus they can be prepared according to the procedures described in US 6262030. Compounds of formula (II), wherein A is -C(O)-, -C(O)NH-, -NHC(O)-, -N(R7)-CH2-, -CH2- 30 N(R7). or -CH(NR8R9h R4 or R5 are hydroxy or R4 and R5 taken together with the intervening atoms form a cyclic group having the following structure: H3C WO 2004/101587 PCTYEP2004/005083 wherein Y is a bivalent radical selected from -O- and -N(R13)-, and R3 is C1-4alkyl, or C3. 6alkenyl optionally substituted by 9 to 10 membered fused blcyclic heteroaryl are known compounds or they may be prepared by analogous methods to those known in the art. Thus they can be prepared according to the procedures described in EP 307177, EP 248279, WO 0078773, WO 9742204. Compounds of formula (II), wherein A is -C(O)NH-, -NHC(O)-, -N(CH3)-CH2- or -CH2- N(CH3)-, R4 or R5 are hydroxy or R4 and R5 taken together with the Intervening atoms form a cyclic group having the following structure: 10 15 and R6 is hydrogen are known compounds or they may be prepared by analogous methods to those known in the art. Thus they can be prepared according to the procedures described in EP 508699 and J.Chem. Res.Synop (1988 pages 152-153), US 6262030. Compounds of formula (II), wherein A is -C(=NR10)-, R4 or R5 are hydroxy or R4 and R5 taken together with the intervening atoms form a cyclic group having the following structure: 20 and R6 is hydrogen, are known compounds or they may be prepared by analogous methods to those known in the art Thus they can be prepared according to the procedures described in EP 284203. Compounds of formula (II), wherein A is -C(O)-, R4 and R5 taken together with the 25 intervening atoms form a cyclic group having the following structure: R6 is hydrogen and R3 is C1-4alkyl may be prepared by decarboxylation of a compound of formula (VIII), wherein R34 is hydroxy protecting group followed, if required, by removal of the protecting group R2 or R34. 30 WO 2004/101587 PCT/EP2004/005083 The decarboxylation may be carried out in the presence of a lithium salt such as lithium 5 chloride, preferably in an organic solvent such as dimethylsulfoxide. Compounds of formula (II), wherein A is -C(0)-, R4 and R5 taken together with the intervening atoms form a cyclic group having the following structure: 10 and R3 is C1-4alky) may be prepared according to the procedures described in WO 02/50091 and WO 02/50092. Compounds of formula (III) wherein X is -U(CH2)v-, in which U is -N(R30)-, -O- or -S-, may be prepared by reaction of XaR11a(V), wherein Xa has the meaning defined above 15 with R35OC(0)(CH2)dL (IX) wherein R35 Is carboxyl protecting group and L is a suitable leaving group, followed by removal of R35. Suitable R35 carboxyl protecting group include t-butyl, allyl or benzyl. In order that the invention may be more fully understood the following examples are given 20 by way of illustration only. The following abbreviations are used in the text: Ac for acetyl, BOC for t-butoxycarbonyl, DCM for dichloromethane, DMAP for 4-dimethylaminopyridine, DMF for N,N- dimethylformamlde, DMSO for dimethyl sulfoxide, EtOAc for ethyl acetate, Me for methyl, 25 MeOH for methanol, TEA for triethylamine and TFA for trifluoroacetic acid. 35 WO 2004/101587 PCT/EP2004/005083 Examples 2'-0-Acetyl-6-0-methyl-erythromycin A may be prepared by the procedure described by W. R. Baker et al. in J. Org. Chem. 1988, 53, 2340, 2'-O-acetyl-azithromycin-11,12- 5 carbonate may be prepared by the procedure described by S. Djokic et al. in J. Chem. Res. (S) 1988, 152 and 11-O-(9E)-methoximino erythromycin A may be prepared according to the procedure described by E. Hunt et al. in J. Chem. Soc, 1989,1726. Nomenclature 15 1 -oxo-6,7-dihydro-1H, 5H-pyrido[3,2,1 -ij]quinoline In the Examples, compounds of formula (I) in which R11 is a tricyclic heterocyclic group are referred to using the numbering system below: 6-oxo-1,2-dihydro-1H,5H-pyrrolo[3,2,1-ij]quinoline 20 Intermediate 1: 7-(3-Amlnopropvl) ,4-dlhvdro-1 -ethvl-6-fiuoro-4-oxo-qulnoline-3- carboxvllc acid sodium salt a) 7-(3-t-Butoxycarbonylamino-prop-1-ynyl)-1,4-dlihydro-1-ethyl-6-fluoro-4-oxo- 25 qulnoline-3-carboxyllc acid ethyl ester. 14-Dihydro-1-ethyl-6-fluoro-7-iodo-4-oxo-quinoline-3-carboxylic acid ethyl ester (0.495 g, 1.265 mmol), copper (I) iodide (26 mg, 013 mmol) and triethylamine (6.16 mL, 44 mmol) were suspended in dry acetonitrile (22 mL). The light green suspension was heated to 50°C whilst argon was bubbled through. After 20 min, 30 dichlorobis(triphenylphosphine)palladium (II) (0.026g, 0.0379 mmol) and N- f-butoxycarbonylpropargylamine (0.341 g, 2.05 mmol) were added and the brown WO 2004/101587 PCT/EP2004/005083 suspension was heated under reflux. After 2 h the reaction mixture was cooled, filtered and concentrated. The residue was taken up in dichloromethane and washed with water. The organic phase was dried and concentrated to provide a brown oil which was purified by chromatography on silica gel eluting with 0-2.5% (9:1 MeOH/20 M NH3) in dichloromethane to yield the title compound as a beige solid; ESMS m/z 417 [M+H]+. 1,4-Dihydro-1-ethyl-6-fluoro-7-lodo-4-oxo-quinoline-3-carboxylic acid can, for example, be prepared by the following method: 10 wherein step (i) is carried out according to the procedure described by C.B. Ziegler, W. V. Curran, N. A. Kuck, S. M. Harris and Y-l Lin in J. Hot Chem., 1989, 26,1141 and step (ii) is carried out using sodium iodide, for example by the method of J. Med. Chem., 2002, 67, 15 843. b) 7-(3-f-Butoxycarbonylaminopropyl)-1,4-dihydro-1 -ethyl-6-fluoro-4-oxo-quinoline- 3-carboxyllc acid ethyl ester. A solution of Intermediate 1a (0.322 mg, 0.77 mmol) In dichloromethane (12 mL) was 20 treated with 10% palladium on carbon (60 mg) and hydrogenated at room temperature and atmospheric pressure overnight. The reaction mixture was filtered and concentrated to yield the title compound as a yellow solid; ESMS m/z 421 [M+H]+. c) 7-(3-Aminopropyl)-1,4-dlhydro-1 -ethyl-6-fluoro-4-oxo-quinoline-3-carboxylic acid 25 ethyl ester. To a solution of Intermediate 1b (254 mg, 0.6 mmol) in dichloromethane (6 mL) was added trifluoroacetic acid (0.66 mL). After 0.75 h at room temperature the reaction mixture was concentrated and the residue was applied to a Varian Bond Elute SCX cartridge. Flushing with MeOH and subsequent elution with 0.04 M NH3. in MeOH up to 2.0 M NH3 30 in MeOH to provided the title compound as a yellow oil; ESMS m/z 321 [M+H]+. d) 7-(3-Aminopropyl)-1,4-dlhydro-1 -ethy!-6-fluoro-4-oxo-qulnoline-3-carboxyllc acid sodium salt. Intermediate 1b (188 mg, 0.59 mmoi) was suspended in 1,4-dioxan (6 mL) and treated 35 with 2N aqueous sodium hydroxide (0.28 mL). The suspension was sonicated for 2 h then treated with excess solid carbon dioxide. Evaporation of the dioxan and filtration of the resultant mixture gave the title compound as a yellow solid. ESMS m/z 293 [M+H]+. WO 2004/101587 PCT/EP2004/005083 Intermediate 2: 6-(3-Aminopropvl)-1.4-dlhvdro-1 -ethvl-4-oxo-auinoHne-3-carboxvlic acid trifluoroacetate salt a) 1,4-Dihydro-1-ethyl-6-iodo-4-oxo-quinoline-3-carboxyllc acid ethyl ester. 5 A mixture of 1,4-dihydro-6-iodo-4-oxo-quinoline-3-carboxylic acid (J. Ellis, E. Gellert, J. Robson. Aust. J. Chem., 1973, 26, 907) (3.15 g, 10 mmol), potassium carbonate (6.9 g, 50 mmol) and iodoethane (15.6 g, 100 mmol) in dry DMF was heated at 70°C with vigorous stirring. After 16 h the mixture was cooled and diluted with ethyl acetate. The resultant mixture was washed with water and the organic phase separated, dried and 10 evaporated to yield the title compound as pale yellow solid, 1H NMR S (CDCI3) 1.41 (3H, t, J = 7.1 Hz), 1.54 (3H, t, J = 7.3 Hz), 4.23 (2H, q, J = 7.2 Hz), 4.40 (2H, q, J = 7.1 Hz), 7.20 (1H, d, J = 8.9 Hz), 7.95 (1H, dd, J = 2.1 & 8.9 Hz), 8.48 (1H, s), 8.86 (1H. d, J = 2.1 Hz). 15 b) 6-(3-f-Butoxycarbonylamino-prop-1-ynyl)-1,4-dlhydro-1-ethyl-4-oxo-qulnollne-3- carboxyllc acid ethyl ester. Using a similar procedure to that described in Intermediate la, a mixture of Intermediate 2a (0.371 g, 1mmol) and N-t-butoxycarbonylpropargylamine (0.264 g, 1.7 mmol) gave the title compound as a yellow solid; ESMS m/z 399 [M+H]+. 20 c) 6-(3-f-Butoxycarbonylamlnopropyl)-1,4-dlhydro-1-ethyl-4-oxo-qulnollne-3- carboxylic acid ethyl ester. Using a similar procedure to that described in Intermediate 1b, Intermediate 2b (0.366 mg, 0.77 mmol) gave the title compound as a yellow oil; ESMS m/z 403 [M+H]+. 25 d) 6-(3-Amlnopropyl)-1,4-dlhydro-1-ethyl-4-oxo-qulnollne-3-carboxyllc acid ethyl ester. Using a similar procedure to that described in Intermediate 1c, Intermediate 2c (355 mg, 0.88 mmol) gave the title compound as a yellow oil; ESMS m/z 303 [M+H]+. 30 e) 6-(3-Aminopropyl)-1,4-dihydro-1-ethyl-4-oxo-qulnollne-3-carboxyllc acid sodium salt. Using a similar procedure to that described in Intermediate 1d, Intermediate 2d (250 mg, 0.83 mmol) gave the title compound as a yellow solid; ESMS m/z 275 [M+H]+. 35 f) 6-(3-Aminopropyl)-1,4-dlhydro-1 -ethyl-4-oxo-qulnoline-3-carboxyllc acid trifluoroacetate salt. Intermediate 2e (0.06 g, 0.2 mmol) was subjected to reverse phase HPLC purification to give the title compound as white solid; 1H NMR δ [(CD3)2SO] 1.54 (3H, t, J = 7.2 Hz), 2.0- 40 2.1 (2H, m), 2.9-3.0 (4H, m), 4.58 (2H, q, J = 7.2 Hz), 7.85,(1H, dd, J = 2.2 & 8.8 Hz), 7.96 (1H, d, J = 8.8 Hz), 8.36 (1H, d, J = 1.8 Hz), 8.97 (1H, s). WO 2004/101587 PCT/EP2004/005083 Intermediate 3: 2'-0-Acetvl-4"-0-propenovl-azlthromvcin-11.12-carbonate A solution of 2'-0-acetyl-azithromycin-11,12-carbonate (10.9 g) in toluene (300 mL) was stirred at room temperature under argon atmosphere. To this solution TEA (12.66 mL) and 5 3-chloro-propionyl chloride (1.94 mL) were added in two portions over a period of 10 minutes. After 20 minutes the solution was diluted with a saturated aqueous solution of NaHC03 (300 mL) and extracted with toluene (4x80 mL). The collected organic phase was dried, filtered and concentrated under reduced pressure affording the title compound (11.0 g). 10 MS;m/z(ES):872[MH]+. Intermediate 4: 4"-0-Propenov[-azlthromvcln-11.12-carbonate A solution of intermediate 3 (11.0 g) in MeOH (200 mL) was stirred at room temperature 15 for 48 h. The solvent was evaporated under reduced pressure affording the title compound (9.81 g). MS; m/z(ES): 829.1 [MH]. 1H-NMR (500 MHz,) 8: 6.45 (d, 1H), 6.17 (dd, 1H), 5.87 (d, 1H), 5.11 (d, 1H), 4.88 (dd, 1H), 4.77 (d, 1H), 4.53 (d, 1H), 4.47-4.40 (m, 3H), 3.72 (m, 1H), 3.60 (d, 1H), 3.33 (s, 3H), 20 3.25 (dd, 1H), 2.87-2.85 (m, 2H), 2.58 (m, 1H), 2.44-2.38 (m, 2H), 2.32 (s, 6H), 2.21 (s, 3H), 2.06 (m, 1H), 2.00 (m, 1H), 1.92 (m, 1H), 1.84 (m, 1H), 170-1.56 (m, 4H), 1.45 (s, 3H), 1.40 (dd, 1H), 1.29 (s, 3H), 1.25 (m, 1H), 1.22 (d, 3H), 1.18 (d, 6H), 1.12 (s, 3H), 108- 1.06 (2d, 6H), 0.93 (m,6H). 25 Intermediate 5; 4"-O-Propenovl-azlthromvcln To a solution of Intermediate 4 (1.3 g) in acetonitrile (50 mL), a saturated aqueous solution of potassium carbonate. (30 mL) was added at room temperature. The resulting mixture was heated to 70°C for 8 h. The mixture was then diluted with water (100 mL), 30 extracted with EtOAc (4x30 mL). The collected organic phase was dried, filtered and concentrated under reduced pressure. The crude product was purified by flash chromatography (eluent: DCM/MeOH/NH3 90/9/0.5) affording the title compound (530 mg). MS; m/z (ES): 804 [MHf. 35 Intermediate 6: 2'-O-Acetvi-4"-0-propenovl-6-0-methvlervthromvcin A To a solution of 2-0-acetyl-6-0-methyl-erythromycin A (1.1 g) in DCM (20 mL) pyridine (1.7 mL) and acryi chloride (1.1 mL) were added at 0°C. After 2 h a further addition of 40 pyridine (1.7 mL) and of acryi chloride (1.1 mL) was performed. The reaction mixture was quenched with a saturated solution of NH4CI (10 mL) and extracted with DCM (3x20 mL). The organic phase was washed with a saturated solution of NaHC03 (10 mL), water (10 WO 2004/101587 PCT/EP2004/005083 mL), dried over Na2S04, filtered and evaporated under reduced pressure. The crude product was purified by flash-chromatography (DCM/MeOH/NH3 95/5/0.5) affording the title compound (470 mg); ESMS m/z 844 [M+H]+. 5 Intermediate 7: 2'-0-Acetvl-6-O-methvl-11-desoxv-11-(R)-amlno-ervthromvcln A 11.12-carbamate To a solution of 6-0-methyl-11-desoxy-11-(R)-amino-erythromycin A 11,12-carbamate (Allhodzlc et a/., WO 03/042228) in dichloromethane (50 mL) was added NaHCOs (478 10 mg) at room temperature. To this solution acetic anhydride (0.153 mL) was added and stirred overnight. To this mixture brine (50 mL) and water (20 mL) were added. The organic layer was separated, washed with brine (20 mL), dried, filtered and evaporated under reduced pressure, affording the title compound (1.2 g). MS; m/z (ES): 816.2 [MH]. 15 Intermediate 8: 2'-0-Acetvl-4"-0-propenovl-6-O-methvl-11-desoxv-11-(R)-amlno- ervthromvcln A 11.12-carbamate Intermediate 7 was dissolved in toluene (50 mL) and the solvent was evaporated. This 20 was performed 2 times. After that the residue was again dissolved in toluene (45 mL) and stirred under argon. To this solution TEA (1.8 mL) and 3-chloropropionylchloride (0.40 mL) (in 3 portions in a period of 20 minutes) were added. 20 min later a saturated aqueous solution of NaHCOs (50 mL) was added. The aqueous solution was extracted with toluene (3x50 mL), the combined organic solution dried over K2C03 and the solvent removed 25 under reduced pressure affording the title compound (1.04 g). MS; m/z (ES): 870.1 [MH]+. Intermediate 9: 4"-0-Propenovl-6-O-methviervthromvcln A 30 Intermediate 6 (1.82 g) was dissolved in MeOH (100 mL) and stirred at 60°C for 4 h, then at room temperature for 16 h. The solvent was evaporated under reduced pressure and the crude product was purified by flash chromatography (eluent: MeOH/DCM/NH4OH 5/90/0) affording the title compound (1.4 g). MS;m/z(ES):802[MH]+. 35 'H-NMR (500 MHz) 8: 6.44 (d, 1H), 6.13 (dd, 1H), 5.89 (d, 1H), 5.07 (d, 1H), 5.00 (d, 1H), 4.75 (d, 1H), 4.60 (d, 1H), 4.38 (m, 1H). 3.97 (s, 1H), 3.80-3.73 (m. 2H), 3.66 (d, 1H), 3.46 (s, 1H), 3.32 (s, 3H), 3.21-3.18 (m, 2H), 3.04 (s, 3 H), 3.00 (m, 1H), 2.92 (m, 1H), 2.56 (m, 2H), 2.43 (d,1H), 2.31 (s,6H). 13C-NMR (75 MHz) 5:221.0; 175.7; 165.8; 131.5; 128.0; 102.1; 96.0; 80.5, 78.8, 78.3; 40 78.0; 76.6; 74.3,72.7; 71.1; 69.1; 67.8; 65.3; 63.2:50.7; 49.5; 45.3; 44.9; 40.3; 39.2; 38.8; 37.2; 35.2; 28.9; 21.7,21.1; 19.7,18.3,18.0,15.9; 12.3; 10.6; 9.1. WO 2004/101587 PCT/EP2004/005083 Intermediate 10: 0-(9E)-Methoxlmino-4"-0-propenovi erythromycin A a) 2'-O-Acetyl-0-(9E)-methoximlno erythromycin. A A solution of 11-0-(9E)-methoximlno erythromycin A (5.7 g, 7.4 mmol) in dichloromethane (70 mL) was treated with triethylamine (1.63 g, 16 mmol) followed by acetic anhydride 6 (1.27 g, 12.5 mmol). After stirring overnight at room temperature the mixture was diluted with dichloromethane and washed with aqueous sodium bicarbonate. The organic layer was separated, dried and evaporated to yield the title product as a solid. ESMS m/z 806 [MH+|. 10 b) 2'-0-Acetyl-(9E)-methoxlmino-4"-O-propenoyl erythromycin A Using a similar procedure to that described in Intermediate 3, intermediate 10a (5.3 g, 6.6 mmol) gave the title compound as a white solid. ESMS m/z 860 [MH+]. c) O-(9E)-Methoximlno-4"-O-propenoyl erythromycin A 15 Using a similar procedure to that described in Intermediate 4, Intermediate 10b (4.17 g, 4.86 mmol) gave the title compound as a white solid. ESMS m/z 818 [MH+]. intermediate 11: 6-(3-Amlnopropvl)-l.4-dlhvdro-1 -ethvl-4-oxo-qulnollne-3- carboxvllc acid sodium salt 20 a) 6-(3-t-Butoxycarbonylamlno-prop-1-ynyl)-1,4-dihydro-1-ethyl-4-oxo-quinoline-3- carboxylic acid ethyl ester 1,4-Dihydro-1-ethyl-6-iodo-4-oxo-quinoline-3-carboxylic acid ethyl ester (0.469 g, 1.265 mmol), copper (I) iodide (26 mg, 0.13 mmol) and triethylamine (6.16 mL, 44 mmol) were 25 suspended In dry acetonltrile (22 mL). The light green suspension was heated to 50°C whilst argon was bubbled through. After 20 min, dichlorobis(triphenylphosphine)palladium (II) (0.026g, 0.0379 mmol) and f-butoxycarbonylpropargylamine (0.341 g, 2.05 mmol) were added and the brown suspension was heated under reflux. After 2 h the reaction mixture was cooled, filtered and concentrated. The residue was taken up in 30 dichloromethane and washed with water. The organic phase was dried and concentrated to provide a brown oil which was purified by chromatography on silica gel eluting with 0- 2.5% (9:1 MeOH/20 M NH3) in dichloromethane to yield the title compound as a beige solid. ESMS m/z 399 (MH+). 35 b) 6-(3-t-ButoxycarbonyFaminopropyl)-1,4-dlhydro-1 -ethyl-4-oxo-qulnoline-3- carboxyllc acid ethyl ester 6-(3-t-Butoxycarbonylaminoprop-1 -ynyl)-1,4-dihydro-1 -ethyl-4-oxo-quinollne-3-carboxylic acid ethyl ester (0.306 g, 0.77 mmol) was dissolved in dichloromethane (12 mL) treated with 10% palladium on carbon (0.06 g) and hydrogenated at room temperature and 40 atmospheric pressure overnight. The reaction mixture was filtered and concentrated to yield the title compound as a yellow solid. ESMS m/z 403 (MH+). WO 2004/101587 PCT/EP2004/005083 c) 6-(3-Amlnopropyl)-1,4-dlhydro-1-ethyl-4-oxo-qulnoline-3-carboxyllc acid ethyl ester 6-(3-t-Butoxycarbonylaminopropyl)-1,4-dihydro-1 -ethyl-4-oxo-quinoline-3-carboxylic acid 5 ethyl ester (0.242 g, 0.6 mmol) was dissolved in dichloromethane (6 mL) and trifiuorocetic acid (0.66 mL) was added. After 0.75 h at room temperature the reaction mixture was concentrated and the residue was applied to a Varian Bond Elute SCX cartridge. Flushing with MeOH and subsequent elution with 0.04 M NH3 in MeOH up to 2.0 M NH3 in MeOH to provided the title compound as a yellow oil. ESMS m/z 303 (MH+). 10 d) 6-(3-Aminopropyl)-1,4-dlhydro-1-ethyl-4-oxo-quinoline-3-carboxyllc acid sodium salt 6-(3-AmlnopropyI)-1,4-dihydro-1-ethyl-4-oxo-qulnollne-3-carboxylic acid ethyl ester (0.179 g, 0.59 mmol) was suspended in 1,4-dioxan (6 mL) and treated with 2M aqueous sodium 15 hydroxide (0.28 mL). The suspension was sonicated for 2 h then treated with excess solid carbon dioxide. Evaporation of the dioxan and filtration of the resultant mixture gave the title compound as a yellow solid. ESMS m/z 275 (MH+). Intermediate 12: 4"-O-[3-[4-(3-Carboxv-1 -ethvl-1.4-dihvdro-4-oxo-6-aulnollnvl) 20 propvlamlnolproplonvll-2'-0-acetvl-(9E)-O-methoximlno erythromycin A Using a similar procedure to that described for the preparation of Example 1 a, Intermediate 10b (0,36g, 0.41 mmol) and Intermediate 2f (0.165 g, 0.41 mmol) gave the title compound. ESMS m/z 1133[MH+]. 25 Intermediate 13: Diethyl 2-((3.4-dlhvdro-2H-qulnolln-1-yl)methvlene)malonate A mixture of tetrahydroquinoline (13.32g, lOOmmol) and diethyl ethoxymethylenemalonate (21.62g, 100mmol) was heated to 130°C using a Dean-Stark apparatus. After 1 hour the reaction mixture was concentrated to give the title compound as a brown oil. ESMS m/z 30 304 (MH+). Intermediate 14: Ethvl 1-oxo-6.7-dlhvdro-1H.5H-pvrldo [3.2.1-ll] qulnollne-2- carboxvlate 35 Intermediate 13 (2.5g, 8.24mmol) was dissolved in polyphosphoric acid and the viscous mixture stirred for 4 hours at 110°C, The reaction mixture was cooled down before adding ice. The resulting precipitate was filtered off, washed with water then dried in a dessicator in the presence of phosphorous pentoxide to give the title compound as a beige solid. ESMS m/z 258 (MH+). 1H NMR (DMSO-d6) 6 8.55(s, 1H), 8.05 (dd, 1H), 7.54 (dd, 1H), 40 7.36 (dd, 1H), 4.27 (q, 2H), 4.22 (q, 2H), 3.00 (t, 2H), 2.10 (tt, 2H), 1.28 (t, 3H). WO 2004/101587 PCT/EP2004/005083 Intermediate 15: Ethyl 9-bromo-1-oxo-6.7-dlhvdro-1H.5H-PVrldo [3.2.1-ll] qulnollne- 2-carboxvlate Intermediate 14 (290mg, 1.13mmol) was dissolved in acetic acid (3mL) and bromine 5 (197mg, 1.23mmol) was added dropwise. The reaction was followed by LC/MS, additional bromine (2 X 197mg) was added. After 24 hours water was added and the precipitate was filtered off, washed with diethyl ether then dried in a dessicator in the presence of phosphorous pentoxide to provide an orange solid which was purified by chromatography on silica gel eluting with 0-1.5% (9:1 MeOH/20 M NH3) in 10 dichloromethane to yield the title compound as a white solid. ESMS m/z 336/338 (MH+). 1H NMR (CDCI3) δ 8.34(d, 1H), 8.31 (s, 1H), 7.48 (d, 1H), 4.37 (q, 2H), 4.17 (t, 2H), 3.03 (t, 2H), 2.23 (tt, 2H), 1.40 (t, 3H). Intermediate 16: Ethvl 9-(3-fert-butoxvcarbonvlamlno-prop-1'Vnvl)-1-oxo-6.7- 15 dihvdro-1W.5H-pvrido [3.2.1-ll] qulnollne-2-carboxvlate A yellow suspension of palladium acetate (73mg, 0.32 mmol) and triphenylphosphine (191mg, 0.72mmol) in dry tetrahydrofuran (6mL) under argon was cooted to 0°C. A solution of n-butyllithium (2.5M in hexanes, 284µL) was added dropwise and after 15 20 minutes the dark green suspension is warmed to room temperature for 15 minutes. This suspension is then cannulated under argon into a white suspension of Intermediate 15 (337mg, 1mmol), copper iodide (84mg, 0.44mmol) and t-butoxycarbonylpropargylamine (198mg, 1.28 mmol) in diethylamine (6mL). The brown suspension is warmed to 45°C for 2 hours then filtered off and preabsorbed on silica gel. Chromatography on silica gel 25 eiuting with 0-5% (9:1 MeOH/20 M NH3) in dichloromethane provided the title compound as a brown oil. ESMS m/z 411 (MH+). 1H NMR (CDCI3) δ 8.23(s, 1H), 8.12 (d, 1H), 7.29 (d, 1H), 5.1 (m, 1H), 4.35 (q, 2H), 4.15 (m, 2x2H), 2.97 (t, 2H), 2.19 (tt, 2H), 1.49 (s, 9H), 1.38 (t,3H). 30 Intermediate 17: Ethvl 9-(3-terf-butoxvcarbonvlamino-propvl)-1-oxo-6.7-dlhvdro- 1H.5H-pvrldo [3.2.1-ll] qulnollne-2-carboxvlate Intermediate 16 (318 mg, 0.77 mmol) was dissolved in dichloromethane (50 mL), treated with 10% palladium on carbon (200 mg) and hydrogenated at room temperature and 35 atmospheric pressure overnight. The reaction mixture was filtered and concentrated to provide a brown oil which was purified by chromatography on silica gel eiuting with 0-1% (9:1 MeOH/20 M NH3) in dichloromethane to yield the title compound as a brown oil. ESMS m/z 415 (MH+). 1H NMR (CDCI3) δ 8.34(s, 1H), 8.11 (bs, 1H), 7.25 (bs, 1H), 4.60 (m, 1H), 4.37 (q, 2H), 4.17 (t, 2H), 3.13 (q, 2H), 3.02 (t, 2H), 2.71 (t,2H), 2.20 (tt, 2H), 40 1.85 (tt, 2H), 1.44 (s, 9H), 1.40 (t, 3H). Intermediate 18: 9-(3-terf-Butoxvcarbonvlamlno-propvn-1 -oxo-6.7-dlhvdro-1 H.5H- pyrldo [3.2.1-Il] qulnoline-2-carboxvllc acid sodium salt WO 2004/101587 PCT/EP2004/005083 Intermediate 17 (240 mg, 0.59 mmol) was dissolved in tetrahydrofuran (3 mL) and treated with 2N aqueous sodium hydroxide (0.32 mL). The solution was heated to 50°C overnight then treated with excess solid carbon dioxide. Evaporation of the solvent gave 5 the title compound as a beige solid. ESMS m/z 387 (MH+). NMR (DMSO-d6) δ 8.83 (s, 1H), 8.11 (bs, 1H), 7.99 (s, 1H), 7.57 (s, 1H), 6.89 (bt, 1H), 4.41 (bt, 2H), 3.04 (t, 2H), 2.94 (q, 2H), 2.71 (L2H), 2.13 (m, 2H), 1.74 (m, 2H), 1.37 (s. 9H). Intermediate 19: 9-(3-Amlno-propvl)-1-oxo-6.7-dlhvdro-1H/.5H-pvrldo [3.2.1-ll] 10 aulnollne-2-carboxvllc acid trtfluoroacetate salt Intermediate 18 (224 mg, 0.58 mmol) was dissolved in trifluoroacetic acid (3 mL). After 0.5 h at room temperature the reaction mixture was concentrated to provide the title compound as a beige solid. ESMS m/z 287 (MH+). NMR (MeOD-d4) δ 8.83 (s, 1H), 15 8.15 (d, 1H), 7.62 (d, 1H), 4.43 (t, 2H), 3.14 (t 2H), 2.98 (t, 2H), 2.89 (L2H), 2.66 (tt, 2H), 2.05 (tt, 2H). Intermediate 20: Diethyl 2-((2.3-dlhvdro-lndol-1-vlmethvlene)rnalonate 20 Using a similar procedure to that described in Intermediate 13 a mixture of indoline (11.9g, lOOmmol) and diethyl ethoxymethylenemalonate (21.62g, 100mmol) at 110°C gave the title compound as a brown oil. ESMS m/z 290 (MH+). Intermediate 21: 6-Oxo-1 .2-dlhvdro-6H-pyrrolo [3.2,1-ll] auinoline-5-carboxvllc acid 25 Using a similar procedure to that described in Intermediate 14 a mixture of Intermediate 20 (28.9g, lOOmmol) and polyphosphoric acid (85g) at 130°C gave the title compound as a brown oil. 1H NMR (DMSO-d6) δ 15.6 (s,1H), 9.11 (s, 1H), 7.97 (dd, 1H), 7.78 (dd, 1H), 7.57 (dd, 1H), 4.77 (t, 2H), 3.57 (t 2H). 30 Intermediate 22 : Ethvl 6-oxo-1.2-dlhvdro-6H-pyrrolo [3.2.1-ll] qulnollne-5- carboxvlate Intermediate 21 (900mg, 4.19mmol) was solublllsed In warm dlmethylformamide (50 mL) 35 then potassium carbonate (2.89g, 20.95mmol) and iodoethane (3.35 mL, 41.9mmoi) were added. The brown suspension was stirred at 70°C for 3 hours then the reaction mixture was concentrated. The residue was taken up In methanol, the solid filtered off and the filtrate preabsorbed on silica gel. Purification by chromatography on silica gel eluting with 0-5% (9:1 MeOH/20 M NH3) in dichloromethane provided the title compound as a beige 40 solid. ESMS m/z 266 (MNa+). 1H NMR (CDCI3) δ 8.57 (s, 1H), 8.08(dd, 1H), 7.46 (dd, 1H), 7.32 (dd, 1H), 4.55 (t 2H), 4.38 (q, 2H), 3.57 (t, 2H), 1.41 (t, 3H). WO 2004/101587 PCT/EP2004/005083 Intermediate 23: Ethvl 8-bromo-6-oxo-1.2-dlhvdro-6H-Pyrrolo [3.2r1-ll] quinollne-5- carboxylate Using a similar procedure to that described in Intermediate 15 a mixture of Intermediate .5 22 (320mg, 1.3mmol), acetic acid (3ml) and bromine (222µL, 4.34mmol) provided the title compound as a yellow solid. ESMS m/z 322/324 (MH+). 1H NMR (CDCI3) δ 8.49(s, 1H), 8.15 (bs, 1H), 7.53 (bs, 1H), 4.56 (t, 2H), 4.36 (q, 2H), 3.56 (t, 2H), 1.39 (t, 3H). Intermediate 24: Ethvl 8-(3-ferf-butoxvcarbonvlamlno-prop-1-ynvl)-6-oxo-1.2- 10 dlhvdro-6H-Pyrrolo [3.2.1-ll] qulnollne-5-carboxvlate Using a similar procedure to that described in Intermediate 16 a mixture of palladium acetate (158mg, 0.69 mmol), triphenylphosphine (415mg, 1.56mmoi), tetrahydrofuran (13mL), n-butyllithium (1.5M in hexanes, 968µL), Intermediate 23 (700mg, 2.17mmol), 15 copper iodide (182mg, 0;96mmol), f-butoxycarbonylpropargylamine (430mg, 2.77 mmol) and dlethylamlne (13mL) provided the title compound as a brown solid. ESMS m/z 397 (MH+). 1H NMR (CDCI3) δ 8.43(s, 1H), 7.98 (bs, 1H), 7.36 (bs, 1H), 4.95 (m, 1H), 4.53 (t, 2H), 4.36 (q, 2H), 4.17 (m, 2H), 3.50 (t, 2H), 1.48 (s, 9H), 1.39 (t, 3H). 20 Intermediate 25: Ethvl 8-(3-terf-butoxvcarbonvlamlno-propvl)-6-oxo-1.2-dlhvdro-6H- pyrrolo [3.2.1-ll] qulnollne-5-carboxvlate Using a similar procedure to that described in Intermediate 17, Intermediate 24 (396 mg, 1 mmol), dichloromethane (80 mL) and 10% palladium on carbon (400 mg) provided the 25 title compound as a yellow oil. ESMS m/z 401 (MH+). 1H NMR (CDCI3) δ 8.54(s, 1H), 7.88 (bs, 1H), 7.32 (bs, 1H), 4.57 (m, 1H), 4.54 (t, 2H), 4.37 (q, 2H), 3.54 (t, 2H), 3.14 (td, 2H), 2.75 (t, 2H), 1.83 (tt, 2H), 1.44 (s, 9H), 1.40 (t, 3H). Intermediate 26: 8-(3-terf-Butoxvcarbonvlamlno-propvl)-6-oxo-1.2-dlhydro-6H- 30 pyrrolo [3.2.1 -il] qulnollne-5-carboxvllc acid sodium salt Using a similar procedure to that described in Intermediate 18, Intermediate 25 (290 mg, 0.72 mmol), tetrahydofuran (3 mL), dioxan (3 mL) and 2N aqueous sodium hydroxide (800 uL) heated at 60°C provided the title compound as a beige solid, ESMS m/z 373 (MH+). 35 1H NMR (DMSO-d6) δ 8.80 (bs, 1H), 7.68 (bs, 1H), 7.48 (bs, 1H), 6.85 (bt, 1H), 4.62 (bt, 2H), 3.51 (t, 2H), 2.95 (td, 2H), 2.73 (t,2H), 1.71 (tt, 2H), 1.34 (s, 9H). Intermediate 27: 8-(3-Amino-propyl)-6-oxo-1.2-dihvdro-6H-Pyrrolo [3.2.1-il] aulnollne-5-carboxylic acid 2.2.2-trlfluoroacetate salt 40 Using a similar procedure to that described in Intermediate 19, Intermediate 26 (285 mg, 0.72 mmol) and trifluoroacetic acid (3mL) provided after FLEX purification the title compound as a pink solid. ESMS m/z 273 (MH+). 1H NMR (DMSO-d6) δ 15.65 (bs, 1H), WO 2004/101587 PCT/EP2004/005083 9.07 (s, 1H), 7.85 (bs, 3H), 7.81 (bs, 1H), 7.66 (bs, 1H), 4.77 (t, 2H), 3.55 (hidden t, 2H), 2.83 (m,4H), 1.91 (tt,2H). Intermediate 28a: 2'.O-Acetyl-O-(M9E)-1-(methvloxy)-2- 5 {{(methyloxy)methylioxy}ethanoxtmlno erythromycin A Using a similar procedure to that described in Intermediate 10a, O-(9E)- 1-(methyloxy)-2- {[(methyloxy)methyl]oxy}ethanoximino erythromycin A (6.25 g, 7.47 mmol) gave the title compound as a white solid. ESMS m/z 880 [MH+]. 10 Intermediate 28b: 2'"O-Acetyl-(9E)-1-(methyloxy)-2-(r(methyloxy)methynoxy}ethano oxlmlno-4"-O-propenoyl erythromycin A Using a similar procedure to that described in Intermediate 3, Intermediate 28a (6.57 g, 15 7.47 mmol) gave the titie compound as a white solid. ESMS m/z 934 [MH+]. Intermediate 28c: O-(9E)-1-(Methyloxy)-2-{{(methyloxy)methyl]oxy}ethano oxlmlno- 4"-O-oropenovl erythromycin A 20 Using a similar procedure to that described in Intermediate 4, Intermediate 28b (5.45 g, 5.84 mmol) gave the title compound as a white solid. ESMS m/z 892 [MH+]. Intermediate 29a: 2'-O-Acetyl-O-(9E)-acetylhydroxlmlno erythromycin A 25 Using a similar procedure to that described in Intermediate 10a, O-(9E)-hydroximino erythromycin A (Tetrahedron Lett,. 1967:1645, 1967) (1.63 g, 1.96 mmol) gave the title compound as a white solid. ESMS m/z 834 [MH+]. Intermediate 29b: 2'-O-Acetyl-(9E)-acetylhydroximlno-4"-O-propenoyl erythromycin 30 A Using a similar procedure to that described in Intermediate 3, Intermediate 29a (1.20 g, 1.35 mmol) gave the title compound as a white solid. ESMS m/z 888 [MH+]. 35 Intermediate 29c: O-(9E)-Oximlno-4"-O-propenoyl erythromycin A Using a similar procedure to that described in Intermediate 4, Intermediate 29b (1.00 g, 1.24 mmol) gave the title compound as a white solid. ESMS m/z 804 [MH+]. 40 General Procedure for the Preparation of Quinolone Esters 30(a-p) WO 2004/101587 PCT/EP2004/005083 A solution of 6-[3-([[(1,1dimethylethyl)oxy]cart]onyl}amino)propyl)-1-ethyl-4-oxo-1,4- dihydro-3-quinolinecarboxyllc acid (0.85 g, 2.27 mmol) and potassium carbonate (0.63 g, 4.54 mmol) in DMF (15 ml) at 60°C-was treated with the requisite alkylating agent (2 equlvs). The reaction was assayed by LC/MS. Once complete, the mixture was cooled 5 and the DMF evaporated and the residue partioned between water and dfchloromethane. The organic phase was separated, dried and evaporated. Chromatography over silica gel eluting with dichloromethane containing an increasing concentration of methanol/ammonium hydroxide gave the N-Boc protected intermediate. After treatment with TFA (1 mL) and evaporation the amine trifluoroacetate salts 30(a-p), described below 10 were obtained. Intermediate 30a: 6-(3-Aminopropyl)-1-ethyl-4-oxo-1,4-dlhvdroqulnoline-3- carboxyilc acid ethyl ester ESMS m/z 303 [MH+]. 15 Intermediate 30b: 6-(3-Aminopropy|)-1-ethvl-4-oxo-1.4-dlhvdroqulnollne-3- carboxvlic acid butvl ester ESMS m/z 431 [MH+]. 20 Intermediate 30c: 6-(3-Aminopropyl)-1-ethvl-4-oxo-1.4-dlhvdroqulnollne-3- carboxyllc acid plvaloyloxymethyl ester ESMS m/z 389 [MH+]. Intermediate 30d: 6-(3-Amlnopropyl)-1-ethyl-4-oxo-1.4-dlhydroauinollne-3- 25 carboxyllc acid 2-(1-N-p|perldlnynethyl ester ESMS m/z 386 [MH+]. Intermediate 30e: 6-(3-Amlnopropyl)-1-ethyl-4-oxo-1.4-dlhydroqulnoline-3- carboxyllc acid 2-methoxyethyl ester 30 ESMS m/z 333 [MH+]. Intermediate 30f: 6-(3-Amlnopropyl)-1-ethvl-4-oxo-1.4-dlhydroaulnollne-3- carboxyllc acid 2-N.N-dlmethyiaminocarbonyl)methyl ester ESMS m/z 360 [MH+]. 35 Intermediate 30g: 6-(3-Amlnooropyl)-1-ethyl-4-oxo.1.4-dlhydroaulnollne-3- carboxyllc acid 2-(1-N-morphlllno)ethyl ester ESMS m/z 388 [MH+]. 40 Intermediate 30h: 6-(3-Amlnopropyl)-1-ethyl-4-oxo-1.4-dlhydroqulnollne-3- carboxyllc acid (ethoxycarbonvlrnethylcarbamoyl)methyl ester ESMS m/z 418 [MH+]. WO 2004/101587 PCT/EP2004/00S083 Intermediate 30l: 6-(3-Amlnopropy)-1-ethyl-4-oxo-1.4-dthydroqulnoline-3- carboxyilc add /-oropyl ester ESMS m/z 317 [MH+]. 5 Intermediate 30l: 6-(3-Amlnopropyl)-1-ethyl-4-oxo-1-4-dlhydroaulnollne-3- carboxylic add Abutyl ester ESMS m/z 331 [MH+], 10 Intermediate 30k: 6-(3-Amlnopropyl)-1-ethyl-4-oxo-1.4-dlhydroqulnollne-3- carboxyllc acid aliyl ester ESMS m/z 315 [MH+]. Intermediate 30m: 6-(3-Amlnopropy1)-1-ethyl-4-oxo-1.4-dlhydroqulnollne-3- 15 carboxyllc acid cycloproqyimethyl ester ESMS m/z 329 [MH+]. Intermediate 30n: 643.Amlnopropyl)-1-ethy|-4-oxo-1.4-dlhydroqulnollne-3- carboxyllc acid 3-butenyl ester 20 ESMS m/z 329 [MH+]. Intermediate 30p: 6-(3-Amlnopropyl)-1-ethyl-4-oxo-1.4-dihydroqulnoline-3- carboxylic acid 2-butynyl ester ESMS m/z 327[MH+]. 25 Intermediate 31: 6-(3-Amlnopropyl)-1.4-dlhydro-1 -methyl-4-oxo-aulnoline-3- carboxyllc acid trlfluoroacetate salt a) 6-{3-t-Butoxycarbonylamlno-prop-1 -ynyl)-1l4-dihydro-4-oxo-qulnollne-3- 30 carboxyllc acid ethyl ester. Using a similar procedure to that described in Intermediate 1a, a mixture of 1,4-dihydro- 6-iodo-4-oxo-quinoline-3-carboxylic acid ethyl ester (J. Tucker, V. Valllancourt; J. Strohbach; K. Romines; M. Schnute; M. Cudahy; S. Thaisrivongs and S. Turner, WO 99/32450) (1.97 g, 5.73 mmol) and N-t-butoxycarbonylpropargylamine (1.34 g, 8.6 mmol) 35 in N.N-dimethyfformamide (50 mL) at 57°C gave the title compound as a cream solid; ESMS m/z 371 [M+H]+. b) 6-(3-t-Butoxycarbonyiamlnopropyl)-1,4-dihydro-4-oxo-quinoline-3-carboxylic acid ethyl ester. 40 Using a similar procedure to that described in intermediate 1b, Intermediate 31a (1.00 g, 2.71 mmol) in dichloromethane:methanol 3:1 (100 mL) gave the title compound as a tan solid; ESMS m/z 375 [M+H]+. 48 WO 2004/101587 PCT/EP2004/005083 c) 6-(3-t-Birtoxycarbonylamlnopropyl)-1,4-dlhydro-1 -methyl-4-oxo-quInollne-3- carboxyilc acid ethyl ester. To a mixture of Intermediate 31b (0.496 g, 1.32 mmol), and potassium carbonate (0.274 5 g, 1.98 mmol) in N,N-dimethylforrnamide (5 mL) was added lodomethane (0.17 mL, 2.65 mmol). After 4.5 h the mixture was diluted with ethyl acetate, filtered, then concentrated In vacuo. The residue was taken up in water, extracted with ethyl acetate, then the organic layers combined, dried (MgS04), filtered, and concentrated in vacuo to give the title compound as a cream solid; ESMS mfz 389 [M+H]+. 10 d) 6-(3-t-Butoxycarbonylamlnopropyl)-1,4-dlhydro-1 -methyl-4-oxo-qulnollne-3- carboxyllc acid. A solution of Intermediate 31c (0.494 g, 1.27 mmol) In tetrahydrofuran (8 mL) was treated with 0.2 N aqueous sodium hydroxide (7.6 mL). After 29 h the mixture was 15 concentrated In vacuo. The resulting residue was taken up in water, treated with excess solid carbon dioxide, and filtered to give the title compound as a cream solid; ESMS mfz 361 [M+H]+. e) 6-(3-Amlnopropyl)-1,4-dihydro-1-methyl-4-oxo-qulnollne-3-carboxylic acid 20 trifluoroacetate salt A solution of Intermediate 31 d (0.388 g, 1.08 mmol) in dichloromethane (6 mL) was treated with trifluoroacetic acid (2 mL). After 35 mln the solvent was removed In vacuo, the residue taken up in toluene (20 mL), the mixture concentrated In vacuo, then the residue taken up in dichloromethane (20 mL), and concentrated In vacuo to give the title 25 compound as a cream solid; ESMS mfz 261 [M+H]+. Intermediate 32: 6-(3-AmlnoDroptl)-1,4-dihydro-1-(2-methoxyethyl)-4-oxo-aulnollne. 3-carboxyllc acid trifluoroacetate salt 30 a) 6-(3-t-utoxycarbonylamlnopropyl)-1,4-dlhydro-1-(2-methoxyethyl)-4-oxo- qulnollne-3-carboxylic acid ethyl ester. To a mixture of Intermediate 31b (0.491 g, 1.31 mmol), sodium carbonate (0.209 g, 1.97 mmol), and sodium Iodide (0.235 gr 1.57 mmol) In N,N-dimethylformamide (5 mL) was added 1-bromo-2-methoxyethane (0.15 mL, 1.57 mmol). After stirring at r.t for 17.5 h the 35 mixture was heated to 67°C for a further 31 h. Additional sodium carbonate (0.050 g, 0.47 mmol) and 1-bromo-2-methoxyethane (0.04 mL, 0.43 mmol) was then added and heating continued for a further 65 h. The mixture was then diluted with ethyl acetate, filtered, and concentrated In vacuo. This residue was taken up in water, extracted with ethyl acetate, then the organic layers combined, dried (MgS04), filtered, and concentrated In vacuo to 40 give a residue which was purified by flash chromatography (silica gel, 0-50% ethyl acetate in dichloromethane) to give the title compound as a cream solid; ESMS mfz 433 [M+H]+. 49 WO 2004/101587 PCT/EP2004/005083 b) 6-(3-t-Butoxycarbonylaminopropy1)-1,4-dlhydro-1 -(2-methoxyethyl)-4-oxo- qulnollne-3-carboxyllc acid. Using a similar procedure to that described in Intermediate 31 d, Intermediate 32a (0.287 g, 0.66 mmol) gave the title compound as a cream solid; ESMS m/z 405 [M+H]+. 5 c) 6-(3-Aminopropyl)-1,4-dlhydro-1 -{2-metrioxyethyl)-4-oxo-qulnollne-3-carboxyllc acid trifluoroacetate salt Using a similar procedure to that described in Intermediate 31 e, Intermediate 32b (0.178 g, 0.44 mmol) gave the title compound as a cream solid; ESMS m/z 305 [M+H]+. 10 Intermediate 33: 6-(3-Amlnopropvl)-1.4-dlhvdro-1-cyclopropyl-4-oxo-qulnollne-3- carboxyllc acid trifluoroacetate salt a) 6-(3-t-Butoxycarbonylamlno-prop-1 -ynyl)-1,4-dihydro-1 -cyclopropyl-4-oxo- 15 quinollne-3-carboxylic acid ethyl ester. Using a similar procedure to that described in Intermediate 1a, a mixture of 1,4-dihydro- 1-cyclopropyi-6-lodo-4-oxo-quinoline-3-carboxylic acid ethyl ester (S. Turner, J. Strohbach; S. Thaisrivongs; V. Vaillancourt; M. Schnute and J. Tucker, WO 00/40561) (0.647 g, 1.69 mmol) and N-t-butoxycarbonylpropargylamine (0.393 g, 2.53 mmol) in 20 acetonltrile (15 mL) at 50°C gave the title compound as a cream solid; ESMS m/z 411 [M+H]+. b) 6-(3-t-Butoxycarbonylamlnopropyl)-1,4-dlhydro-1 -cyclopropyl-4-oxo-qulnollne-3- carboxyllc acid ethyl ester. 25 Using a similar procedure to that described in Intermediate 1b, Intermediate 33a (0.450 g, 1.10 mmol) in dichloromethane (20 mL) gave the title compound as a cream solid; ESMS m/z 415 [M+H]+. c) 6-(3-t-ButoxycarbonyIaminopropyl)-1,4-dihydro-1-cyclopropyl-4-oxo-qulnollne-3- 30 carboxyllc acid. Using a similar procedure to that described in Intermediate 32b, Intermediate 33b (0.447 g, 1.08 mmol) gave the title compound as a cream solid; ESMS m/z 387 [M+H]+. d) 6-(3-Aminopropyl)-1,4-dlhydro-1-cyclopropyl-4-oxo-qulnoline-3-carboxylic. acid 35 trifluoroacetate salt Using a similar procedure to that described In Intermediate 32c, Intermediate 33c (0.392 g, 1.01 mmol) gave the title compound as a cream solid; ESMS m/z 287 [M+H]+. Intermediate 34: 6-Prop-2-vnvloxv-hexanolc acid ethyl ester 40 To a solution of 6-hydroxy-hexanolc acid ethyl ester (0.5 mL, 3.1 mmol) In THF (5 mL) was added tetrabutylammonium iodide (57.2 mg, 0.155 mmol), sodium Iodide (69.7 mg, 50 WO 2004/101587 PCT/EP2004/005083 0.465 mmol), 3-bromo-propyne (518 µl, 4.65 mmol) and potassium hydroxide (173.9 mg, 3.1 mmol) and the mixture was stirred for 5 hours at room temperature. The solvent was evaporated and the residue extracted with EtOAc and water (2x20 mL). The organic layer was washed with NaCl (2x20 mL), dried over K2CO3 and evaporated In vacuo yielding 5 (0.347 g) of the title product. MS (ES)m/z[MH]+ 199.27. 1H NMR (500 MHz, DMSO) δ ppm: 4.09 (2H, CH2), 4.04 (2H, CH2), 3.40 (2H, CH2), 3.37 (H, C-CH), 2.27 (2H, CH2), 1.48 (4H, 2xCH2), 1.30 (2H, CH2), 1.17 (3H. CH3). 13C NMR (300 MHz, DMSO) δ ppm: 177.40, 85.14, 81.44, 73.57, 64.26, 61.89, 37.99, 10 33.21,29.80,28.88,18.74. Intermediate 35; 6-[3-(5-Ethoxvcarbonyl-Dentyloxy)-Drop-1-ynynl]1-ethyl~4-oxo-1.4. dlhydro-qunollne-3-carboxyllc acid ethyl ester 15 Intermediate 2a (312 mg, 0.84 mmol), copper(l) iodide (16 mg, 0.084 mmol) and triethylamlne (4.072 mL, 29.4 mmol) were suspended in dry acetonitrile (10 mL). The suspension was heated to 50°C and N2 bubbled through. After 20 min, dichlorobls(triphenylphosphine) palladium (II) (18 mg, 0.0252 mmol) and Intermediate 34 (347 mg, 1.75 mmol) were added and the suspension was stirred at 50 °C for 4 hours. 20 The solvent was evaporated and the residue was extracted with EtOAc and water (2x50 mL). The organic layer was washed with NaCI and NaHC03 (2x50 mL), dried over K2C03 and evaporated In vacuo yielding (476 mg) of the title product MS (ES)m/z:[MH]+442.25. 1H NMR (500 MHz, DMSO) δ ppm: 8.70 (1H, Q), 8.23 (1H, Q), 7.82 (2x1 H, Q), 4.43 (2H, 25 Q-N-CH2-CH3), 4.40 (2H, CH2), 4.23 (2H,Q-CO2-CH2-CH3), 4.03 (2H, CH2), 3.52 (2H, CH2), 2.29 (2H, CH2), 1.55 (4H, 2xCH2), 1.36 (3H, Q-N-CH2-CH3), 1.34 (2H, CH2), 1.29 (Q-CO2-CH2-CH3), 1.16 (3H, CH3). 13C NMR (300 MHz, DMSO) δ ppm: 172.73, 171.87, 164.33, 149.20, 138.32, 134.88, 129.34, 128.15, 118.20, 117.94,110.55, 87.26, 84.29, 69.11, 59.74, 59.54, 57.82, 47.93, 30 33.37,28.57, 25.10, 24.17,14.23,14.02. Intermediate 36: 6-[3-(5-Carboxy-pentyloxy)-prop-1-ynyl]'1-ethyl-4-oxo-1.4-dlhydro- aulnoline-3-carboxylic acid 35 To a solution of Intermediate 35 (476 mg, 1.08 mmol) in THF (5.55 mL) was added a solution of sodium hydroxide (185 mg, 4.62 mmol) In water (5.5 mL) and the mixture was stirred for 2 hours at 80°C and for 12 hours at room temperature. The reaction mixture was extracted with EtOAc and water (2x20 mL). The pH value of water layer was adjusted from 9.8 to 5.2 by adding of 2N HCI and the layer was extracted with DCM. The organic 40 layer was evaporated In vacuo yielding (184 mg) of the title product MS (ES)m/z:[MH]+386.19. 51 WO 2004/101587 PCI7EP2004/005083 Intermediate 37: 2'-Acetyl-4'-O-{6-[3-(3-carboxy-1-ethy1-4-oxo-1.4-dlhydro-qulnolln- 6-yl)-prop-2'ynuloxy1-hexanoyl)-azlthromycin To a solution of Intermediate 36 (184 mg, 0.48 mmol) in dry DMF (6 mL), which was 5 cooled to. 0°C and N2 bubbled through, was added 1-(3-dlmethylamlnopropyl}-3- ethyicarbodilmide hydrochloride (131.8 mg, 0.69 mmol) and a solution of 2'OAc- azithromycin (237.3 mg, 0.3 mmol) in dry DCM (3 mL) was added dropwise. DMAP (61.6 mg, 0.504 mmol) was then added to the reaction mixture. The suspension was stirred at first for 3 hours at 0°C and then gradually up to room temperature for 24 hours. The 10 solvent was evaporated and residue was extracted with EtOAc and water (2x20 mL). The organic layer was dried over K2C03 and evaporated in vacuo yielding (325 mg) of the title product MS (ES) m/z: [MH]* 1158.63. Intermediate 38: 4"-O-{6-[3-(3-Carboxy-1-ethyl-4-oxo-1.4-dlhydro-qulnolln-6-yl)- 15 prop-2-vnyloxy1-hexanoyl}-azltr}romycln A solution of Intermediate 37 (325 mg, 0.28 mmol) in methanol (40 mL) was heated to 55 °C for 12 hours. The solvent was evaporated and the residue was purified by column chromatography (DCM: MeOH: NH3= 90:5:0.5) yielding (106 mg) crude yellow product. 20 MS (ES) m/z: [MH]+ 1117.08. Intermediate 39: 2'-Acetyl-4"-O-{6-[3-(3-carboxy-1-ethyl-4-oxo-1.4-dihydro-qulnolln- . 6-yl)-prop-2-ynyloxy]-hexanoyl]-clarlthromycln 25 Using a similar procedure to that described in Intermediate 37, Intermediate 36 (148 mg, 0.38 mmol) and 2'OAc-clarithromycln (233 mg, 0.3 mmol) in dry DCM (3 mL) gave the title product (302 mg). Intermediate 40: 4"-O-{6-[3-(3-Carboxy-1-ethyl-4-oxo-1.4-dlhydro-qulnolln-6-yl)- 30 prop-2-ynyloxyl-hexanovll-clarrthromycln Using a similar procedure to that described in Intermediate 38, Intermediate 39 (302 mg, 0.26 mmol) gave the title product as a yellow solid (78mg). MS (ES) m/r [MH]* 1115.55. 35 Intermediate 41: 2'-Acetyl-4"-O-{6-[3-(3-carboxy-1-ethyl-4-oxo-1.4-dihydro-quinolln- 6-yl)-prop-2-tnyloxy]-hexanoyl)11 -O-Me-azithromycin Using a similar procedure to that described in Intermediate 37, Intermediate 36 (280 mg, 40 0.73 mmol) and 2'OAc-11-O-Me-azithromycin (Kobrehel et a/., J. Antibiotics, 1982, 45, 527) (489 mg, 0.61 mmol) in dry DCM (5 mL) gave the title product (250 mg). 52 WO 2004/101587 PCT/EP2004/005083 Intermediate 42: 4"-O-{6-[3-(3-Carboxy-1-ethyl-4-oxo-1.4-dlhydro-qulnolin-6-yl)- prop-2-ynyloxy]-hexanoyl]-11 -O-Me-azithromycIn Using a similar procedure to that described in Intermediate 38, Intermediate 41 (250 mg, 5 0.21 mmol) gave the title product as a yellow solid (141 mg). MS (ES) m/r [MH]+ 1130.56. Example 1; 4"-O-{3-[3-(3-Carboxy-1.4-dlhydro-1-ethyl-6-fluoro-4-oxo-7-quinolinyl) propylamlnolproplonyl}-6-O-methylerythromycinA a) 2'-O-Acetyl-4"-0-{3-[3-(3-carboxy-1,4-dlhydro-1 -ethyl-6-fluoro-4-oxo-7-quInollnyl) propylamlno]proplonyl}-6-O-methyterythromyclnA. A mixture of Intermediate 6 (0.089 g, 0.1 mmoi) and Intermediate 1 trifluoroacetate salt 15 (0.1 g, 0.25 mmol) in DMSO (3 mL), water (5 drops) and triethylamine (0.127 g, 1.25 mmol) was heated at 80°C. After 3 days additional Intermediate 6 (0.089 g, 0.1 mmoi) was added and the mixture heated for a further 2 days. The mixture was cooled, partitioned between dichloromethane and water and the organic phase dried and concentrated. The residue was chromatographed over silica gel eluting with 0-2.5% (9:1 20 MeOH/20 M NH3) in dichloromethane to yield the title compound as a white solid; ESMS m/z 1136[M+H]+. b) 4"-O-{3-[3-(3-Carboxy-1,4-dlhydro.1-ethyl-6-fluoro-4-oxo-7-qulnollnyl) propylamlno]propionyl}-6-0-methylerythromycinA. 25 Example 1a (0.04 g, 0.035 mmol) was dissolved in methanol (5 mL) and heated to 50°C for 24 h. The reaction mixture was concentrated to provide the title compound as a beige solid; ESMS m/z 1094 [M+H]+. Example 2: 4"-O-{3-[3-(3-Carboxy-1.4-dihydro-1-ethyl-6-fluoro-4-oxo-7-qulnollnyn 30 propylamlnolproplonyltazlthromycln tris trifluoroacetate salt 53 WO 2004/101587 PCT/EP2004/005083 Intermediate 1 (0.117 g, 0.34 mmol) and Intermediate 5 (0.273 g, 0.34 mmol) in methanol (3 mL) were heated at 64°C overnight The reaction mixture was 5 chromatographed over silica gel eluting with 0-10% (9:1 MeOH/20 M NH3) in dichloromethane followed by reverse phase HPLC purification to yield the title compound as a white solid; ESMS m/z 1095 [M+H]+. Example 3: 4"-O-{3-[3-(3-Carboxy-1.4-dlhydro-1-ethyl-6-fluoro-4-oxo-7-qulnolinyl 10 propylamlnolproplonyl]-azlthromycln-11.12-carbonate Intermediate 4 (0.282 g, 0.34 mmol) and Intermediate 1 (0.117 g, 0.34 mmol) in 15 isopropanol (4 mL), water (1 drop) and triethylamine (0.069 g, 0.68 mmol) were heated at 80°C. After 3 days DMSO (2 mL) was added and the mixture heated overnight, the reaction mixture was diluted with methanol and purified by reverse phase HPLC followed by chromatography over silica gel eluting with 0-5% (9:1 MeOH/20 M NH3) in dichloromethane to yield the title compound as a white solid; ESMS m/z 1121 [M+H]+. 20 Example 4: 4"-O-{3-[3-(3-Carboxy-1.4-dlhydro-1-ethyl-4-oxo-6-qulnoHnyn propylamino]proplonyl}-6-O-methylerythromycin A 54 WO 2004/101587 PCT/EP2004/005083 Using a similar procedure to that described in Example 1a, Intermediate 9 (0.120 g, 0.15 mmol) and Intermediate 2 (0.058g, 0.15 mmol) gave the title compound as a white solid; 5 1H NMR 8 (CDCI3) inter alia 4.99 (1H, d, J = 5.0 Hz), 5.06 (1H, d, J = 8.9 Hz), 7.57 (1H, d, J = 8.8 Hz), 7.69 (1H, d x d, J = 2.1 & 8.7 Hz), 8.37 (1H, d, J = 2.0 Hz), 8.77 (1H, s); ESMS m/z 1076 [M+H]+. Example 5: 4"-O-{3-[3-(3-Carboxy-1.4-dihydro-1-ethyl-4-oxo-6-auinollnyl) 10 propylamlnoTpropionyll-azithromycln Using a similar procedure to that described in Example 1a, Intermediate 5 and Intermediate 2e (0.082 g, 0.26 mmol) gave the title compound as a white solid; ESMS m/z 1077 [M+H]+. Example 6: 4"-O-{3-[3-(3-Carboxy-1.4-dlhydro-1-ethyl-4-oxo-6-aulnoluinyl) propylamlno]proplonyl]-azlthromycin-11.12-carbonate 55 Using a similar procedure to that described in Example 1a, Intermediate 4 (0.216 g, 0.26 mmol) and Intermediate 2e (0.082 g, 0.26 mrnol) gave the title compound as a white solid; 1H NMR δ (CD3OD) Inter alia 4.85 (1H, d, J = 6.0 Hz), 5.09 (1H, d, J = 4.4 Hz), 7.78 (1H, d, J = 8.8 Hz), 7.86 (1H, d, J = 8.8 Hz), 8.30 (1H, d, J = 1.6 Hz), 8.84 (1H, s); ESMS m/z1103[M+H]+. Example 7: 4"-O-{3-[3-(3-Carboxy.1.4-dihydro-1 -ethyl-4-oxo-6-aulnollnyl) Dropylamlno1proplonyl}-6-0-m9thyl-11-desoxy-11-(R)-amlno-erythromycln A 11.12- carbamate dtformate salt a) 4"-O-{3-[3-(3-Carboxy-1,4-dihydro-1 -ethyl-4-oxo-6-qulnolinyl)propylamlno] propIonyl-2'-0-acetyl-6-0-methyl-11-desoxy-11-(R)-amino-erythromycln A 11,12- carbamate. Using the procedure described in Example 1a, Intermediate 8 (0.108 g, 0.125 mmol) and Intermediate 2e gave, after chromatography, the title compound as white solid; ESMS m/z 1143 [M+H]+. Also Isolated was 4"-0-{3-[3-(3-carboxy-1,4-dihydro-1-ethyl-4-oxo-6- quinolinyl)propylamlno]proplonyl-2-0-acetyi-6-O-methyl-1 1 -desoxy-11 -(R)-amino- erythromycin A 11,12-carbamate methyl ester, obtained as a white solid; ESMS m/z 1157 [M+H]+. b) 4"-O-{3-[3-(3-Carboxy-1,4-dihydro-1 -ethyl-4-oxo-6-qulnollnyl)propylamlno] WO 2004/101587 PCT/EP2004/005083 proplonyl-6-O-methyl-11-desoxy-11-(R)-amlno-erythromycIn A 11,12-carbamate dlformate. Using the procedure described in Example 1b, Example 7a was converted to the title compound. Purification by reverse phase HPLC gave a white solid; 1H NMR 8 (CD3OD) 5 Inter alia 4.98 (1H, d, J = 4.8 Hz), 5.05 (1H, d, J = 8.4 Hz), 7.84 (1H, d x d, J = 2.0 & 9.2 Hz), 7.95 (1H, d, J = 8.8 Hz), 8.36 (1H, d, J = 1.6 Hz), 8.97 (1H, s); ESMS m/z 1102 [M+H]+. Example 8: 4"-O-{3-[3-(3-Carboxy-1.4-dihydro-1-ethyl-4-oxo-6-aulnolinyl) 10 propylaminolproplonyl}-6-0-methyl-11 -desoxy-11 -(R)-amlno-erythromycin A 11/12- carbamate methyl ester dlformate salt 15 Using the procedure described in Example 1b, 4"-O-{3-[3-(3-carboxy-1,4-dihydro-1-ethyl- 4-oxo-6-quinolinyl)propylamino]propionyl-2'-O-acetyl-6-0-methyl-11-desoxy-11-(R)-amino- erythromycin A 11,12-carbamate methyl ester obtained in Example 7a was converted to the title compound. Purification by reverse phase HPLC gave a white solid; ESMS m/z 1116[M+H]+. 20 Example 9: 4"-O{3-[4-(3-Carboxy-1 -ethyl-1.4-dihydro-4-oxo-6-auinollnyl) propylamlnolproplonyl]-(9E)-O-methoxlmlno erythromycin A 57 WO 2004/101587 PCT/EP2004/005083 Using a similar procedure to that described for the preparation of Example 1b, Intermediate 12 (0.14 g, 0.12 mmol) gave the title compound as a white solid ESMS m/z 1091 [MHJ+. Example 10: 4"-O-{3.[4.(2-Carboxy-6.7-dlhydro.1H,5H-pyrido[3.2.1-l]-1-oxo-9- qulnollnyl)propylamlno]proplonyl].6-O-methyl erythromycin A A mixture of Intermediate 9 (0.116 g, 0.145 mmol) and Intermediate 19 (0.116 g, 0.29 mmol) in DMSO (1 mL), water (1 drop) and triethylamlne (0.13 µL, 0.9 mmol) was heated at 80°C. After 2 days the mixture was cooled and submitted to Mass Directed Auto Prep purification followed by chromatography over silica gel eluting with 0-5% (9:1 MeOH/20 M NH3) in dichloromethane to yield the title compound as a white solid. ESMS m/z 1089 (MH+). NMR (MeOD-44) 5 8.70 (s, 1H), 8.07 (bs, 1H), 7.55 (bs, W), 5.13 (dd, 1H), later alia. Example 11: 4"-O-{3-[4-(2-Carboxy-6.7-dihydro-1H.5H-pyrldo[3.2.1-il]-1-oxo-9- qulnolinyl) propylamino]proplonyl]azithromycln tris formate salt A mixture of Intermediate 5 (0.088 g, 0.11 mmol) and Intermediate 19 (0.066 g, 0.165 mmol) in DMSO (1 mL), water (1 drop) and triethylamlne (0.072 µL, 0.49 mmol) was heated at 80°C overnight. The mixture was cooled and submitted to Mass Directed Auto Prep purification to yield the title compound as a white solid. ESMS m/z 1090 (MH+). 58 WO 2004/101587 PCT/EP2004/005083 NMR (MeOD-d4) δ 8.82 (s, 1H), 8.45 (s, 3H), 8.15 (bs, 1H), 7.63 (bs, 1H), 5.10 (d. 1H), Inter alia. Example 12: 4"-O-{3-[4-(2-Carboxy-6.7.dlhydro-1H.5H.Dyrido[3.2.1.ll]-1-oxo-9. 5 qulnollnyl)propylamlno]proplonyl]azithromycln-11.12-carbonate 10 15 A mixture of Intermediate 4 (0.120 g, 0.145 mmol) and Intermediate 19 (0.116 g, 0.29 mmol) in DMSO (1 mL), water (1 drop) and triethylamine (0.13 µL, 0.9 mmol) was heated at 80°C. After 2 days the mixture was cooled and submitted to Mass Directed Auto Prep purification followed by chromatography over silica gel eluting with 0-5% (9:1 MeOH/20 M NH3) In dichloromethane to yield the title compound as a white solid. ESMS m/z 1116 (MH+). NMR (CDCI3) δ 8.65 (s, 1H), 8.15 (d, 1H), 7.44 (d, 1H), 5.10 (d, 1H), inter alia. Example 13: 4"-O-{3-[4-(5-Carboxy-1.2-dlhydro-6H-pyrroio[3.2.1-ll]-6-oxo-8- aulnollnyl) propylarnlno]proplonyn-6-O-rnethyl erythromycin A bis formate salt 20 25 Using a similar procedure to that described in Example 10 a mixture of Intermediate 9 (0.160 g, 0.2 mmol) and Intermediate 27 (0.094 g, 0.24 mmol), DMSO (1 mL), water (1 drop) and triethylamine (0.104 µL, 0.72 mmol) provided after Mass Directed Auto Prep purification the title compound as a beige solid. ESMS m/z 1075 (MH+). NMR (MeOD-d4) δ 9.01 (s, 1H), 8.37 (s, 2H), 7.91 (bs, 1H), 7.67 (bs, 1H), 5.15 (dd, 1H), Inter alia. 59 WO 2004/101587 PCT/EP2004/005083 Example 14: 4"-O-{3-[4-(5-Carboxy-1.2-dlhydro-6H.pyrrolo[3.2.1-ll]-6-oxo-8- aulnollnyl) propylamlno]proplonyl]azlthromycln tris formate salt Using a similar procedure to that described in Example 11 a mixture of Intermediate 5 (0.051 g, 0.063 mmol), Intermediate 27 (0.039 g, 0.1 mmol), DMSO (2 mL), water (1 drop) and triethyiamine (0.300 µL, 2.04 mmol) provided after Mass Directed Auto Prep purification the title compound as a white solid. ESMS m/z 1076 (MH+). NMR (MeOD-d4) 8 9.0 (s, 1H), 8.39 (s, 3H), 7.91 (bs, 1H), 7.67 (bs, 1H), 5.10 (d, 1H), Inter alia. Example 15: 4"-O-[3-[4-(5-Carboxy-1.2-dlhydro-6H-pyrrolor3.2.1-ll]-6-oxo-8- qulnollnyl) propylamlno]proplonyl]azlthromycln-11,12-carbopate tris formate salt Using a similar procedure to that described in Example 12 a mixture of Intermediate 4 (0.1 g, 0.2 mmol), Intermediate 27 (0.094 g, 0.24 mmol), DMSO (1 mL), water (1 drop) and triethyiamine (0.104 µL, 0.72 mmol) provided after Mass Directed Auto Prep purification the title compound as a beige solid. ESMS m/z 1102 (MH+). NMR (MeOD-d4) δ 8.93 (s, 1H), 8.87 (s, 1H), 8.39 (s, 3H), 7.66 (s, 1H), 5.09 (d, 1H), Inter alia. Example 16: 4"-O-[3-[4-(2-Carboxy-6.7-dlhydro-1H.5H-pyrido[3.2.1-ll]-1-oxo-9- quinollnvl) propylamlno]proplonyl]-(9E)-O-methoxlmlno erythromycin A blsformate 60 WO 2004/101587 PCT/EP2004/005083 Using a similar procedure to that described in Example 1a, Intermediate 10c and Intermediate 19 gave the title compound as a white solid; ESMS 1104 m/z [M+H]+. Example 17: 4"-O-[3-[4-(3-Carboxy-1-ethyl-1.4-dihydro-4-oxo-6-qulnolinyl) propylamlno]proplonyl]-(9E)-O-({[2-(methyloxy)ethynoxy}methanoxlmlno erythromycin A blsformate Using a similar procedure to that described in Example 1a, Intermediate 28c and Intermediate 2 gave the title compound as a white solid; ESMS m/z 1166 [M+H]+. Example 18: 4"-O-[3-[4-(2-Carboxy-6.7-dlhydro-1H,5H-pyrido[3.2.1-il]-1-oxo-9- aulnollnyl>propylairilno]propionyn-(9E)-O-({[2. (methyloxy)ethyfloxy}methanoxlmlno erythromycin A blsformate 61 WO 2004/101587 PCT/EP2004/005083 '■V—9 Using a similar procedure to that described in Example 1a, Intermediate 28c and Intermediate 19 gave the title compound as a white solid; ESMS m/z 1178 [M+H]+. 5 Example 19: 4"-O-[3-[4-(3-Carpoxy-1 -ethyl-1.4-dlhydro-4-oxo-6-qulnollnyl) propylamlno]proplonyl]-(9E)-O-hydroxlmlno erythromycin A Using a similar procedure to that described in Example 1a, Intermediate 29b and 10 Intermediate 2 gave the title compound as a white solid; ESMS m/z 1078 [M+H]+. Example 20: 4"-O-[3-[4-(2-Carboxy-6.7-dlhydro-1H.5H-Pyrldo[3`.2.1-ll]-1-oxo-9- qulnollnyl) propylamlno]proplonyl]-(9E)-O-hydroxlmlno erythromycin A 15 62 WO 2004/101587 PCT/EP2004/005083 Using a similar procedure to that described in Example 1a, Intermediate 29c and Intermediate 19 gave the title compound as a white solid; ESMS m/z 1090 [M+H]+. General Procedure for the preparation of Esters of Example 4 5 The title compounds were prepared as described in Example 1a from Intermediate 9 (0.54 g, 0.64 mmol) and the requisite quinolone 3-carboxylic ester, Intermediate 30(a-p) (1.28 mmol). Once complete, as determined by LC/MS, the reaction was cooled and partitioned between water and dichloromethane. The organic layer was separated, dried and evaporated to yield the crude product. Chromatography over silica gel eluting with 10 dichloromethane containing an increasing concentration of methanol/ammonlum hydroxide (0 to 10 %) gave the compounds described below. Example 21: 4"-O-{3-[3-(3-Carboxy-1.4-dihydro-1-ethyl-4-oxo-6-qulnollnyl) propylamlno]propionyl}-6-O-methylerythromycln A ethyl ester 15 ESMS m/z 1105 [MH+]. 20 Example 22: 4"-O-{3-[3-(3-Carboxy-1.4-dihydro-1-ethyl-4-oxo-6-qulnollnyl) propylamlno]propionyl]-6-O-methyierythromycin A n-butyl ester 63 WO 2004/101587 PCT7EP2004/005083 ESMS m/z 1133[MH+J. 5 Example 23: 4"-O-{3-[3-(3-Carboxy-1.4-dihydro-1-ethyl-4-oxo-6-qulnollnyl) propylamino]proplonyl}-6-O-methylerythromycln A pivaloyloxymethyl ester ESMS m/z 1191 [MH+J. 10 Example 24: 4"-O-{3-[3-(3-Carboxy-1.4-dlhydro-1-ethyl-4-oxo-6-quinollnyl) propylamlno]proplonyl]-6-O-methylerythromycin A 2-(1 -N/piperldinyl)ethyl ester 64 WO 2004/101587 PCT/EP2004/005083 > 0 ESMS m/z 1188[MH+]. Example 25: 4"-O-{3-[3-(3-Carboxy-1.4-dlhydro-1-ethyl-4-oxo-6-gulnollnyl) 5 propylamlno]proplonyl]-6-O-methylerythromycin A2-methoxyethyl ester ESMS m/z 1135[MH+l. 10 Example 26: 4"-O-{3-[3-(3-Carboxy-1.4-dlhydro-1-ethyl-4-oxo-6-quinollnyl) propylamlno]proplonyl]-6-0-methylerythromycln A 2-(N.N- dlmethlyamlnocarbonyl)methyl ester 65 WO 2004/101587 PCIYEP2004/005083 ESMS m/z 1162[MH+]. Example 27: 4"-O-{3-[3-(3-Carboxy-1.4-dlhydro-1-athyl-4-oxo-6-quinollnyl) 5 propylamlno]proplonyl]-6-0-methylerythromycln A 2-(1-N-morphlllno)ethyl ester ESMS m/z1140 MH+J. 10 Example 28: 4"-O-{3-[3-(3-Carboxy-1.4-dlhydro-1-ethyl-4-oxo-6-qulnollnyl) propylamlno]proplonyl]-6-O-methylerythromycln A 2-(2-(ethoxy)-2- oxoethylamlnocarbonyl]ethyl ester 66 WO 2004/101587 PCT/EP2004/005083 ESMS m/z 1220[MH+]. Example 29: 4"-O-{3-[3-(3-Carboxy-1,4-dlhydro-1-ethyl-4-oxo-6-qulnolinyl) 5 propylamino]proplonyl}-6-0-methylerythromycln A /-propyl ester ESMS m/z 1119[MH+]. 10 Example 30: 4"-O-{3-[3-(3-Carboxy-1.4-dihydro-1-ethyl-4-oxo-6-qulnollnyl) propylamlnolproplonyl]-6-O-methylerythromycin A /-butyl ester 67 WO 2004/101587 PCT/EP2004/005083 ESMS m/z 1134[MH+]. Example 31: 4".O-{3-[3-(3-Carboxy-1.4-Cahydro-1-ethyl-4-oxo-6-qulnolinyl) 5 propylamlno]proplonyl]-6-O-methylerythromycln A allyl ester ESMS m/z 1117[MH+]. 10 Example 32: 4".O-{3-[3-(3-Carboxy-1.4-dIhydro-1-ethyl-4-oxo-6-qulnollnyl) propylamlno]proplonyl]-6-O-methylerythromycln A cyclopropylmethyl ester 68 WO 2004/101587 PCT/EP2004/005083 ESMS m/z 1131[MH+]. Example 33: 4"-O-{3-[3-(3-Carboxy-1.4-dihydro-1-ethyl-4-oxo-6-aulnollnyl) 5 Dropylamlno]proplonyl]-6-O-methylervthromycln A 3-butenyl ester ESMS m/z 1131 [MH+J. 10 Example 34: 4"-O-{3-[3-(3-Carboxy-1.4-dlhydro-1-ethyI-4-oxo-6-qulnollnyl) propylamlno]proplonyl]-6-O-methylerythromycln A 2-butynyl ester WO 2004/101587 PCT7EP2004/005083 ESMS m/z 1129 [MH+]. Example 35: 4"-O-{3-[3-(3-Carboxy-1.4-dlhydro-1-ethyl-4-oxo-6-qulnollnyl) propylamlno]proplonyl}6-O-methyl-11-desoxy-11-(R)-amlnomethyl-erythromycin A 11.12-carbamate dlformate salt Using a similar procedure to that described in Example 1a, Intermediate 2 (0.090 g, 0.23 mmol) and Intermediate 31 (S. Alihodzic et a/., WO 03/042228) (0.103 g, 0.23 mmol) gave, after chromatography, methanoiysls of the 2'OAc, followed by chromatography gave the title compound as a cream solid; ESMS m/z 1116 [MH]. Example 36: 4"-O-{3-[3-(3-Carboxy-1.4-dlhydro-1-methyl-4-oxo-6-qulnollnyl) propylamlno]proplonyl]-azlthromycln-11.12-carbonate 70 WO 2004/101587 PCT/EP2004/005083 Using a similar procedure to that described in Example 1a, Intermediate 31e and Intermediate 4 gave the title compound as a white solid; ESMS 1090 m/z [M+H]+. Example 37: 4"-O-{3-[3-(3-Carboxy-1.4-dlhydro-1 -methyl-4-oxo-6-quinollnyl) propylamlno]proplonyl]-6-O-methylerythromycln A Using a similar procedure to that described in Example 1a, Intermediate 32c and Intermediate 9 gave the title compound as a white solid; ESMS 1063 m/z [M+H]+. Example 38: 4"-O-{3-[3-(3-Carboxy-1.4-dlhydro-1 -(2-methoxyethyl)-4-oxo-6- 71 quinollnyl) propylamlno]proplonyl]-6-O-methylerythromycln A WO 2004/101587 PCT/EP2004/005083 Using a similar procedure to that described in Example 1a, Intermediate 32c and Intermediate 9 gave the title compound as a white solid; ESMS 1107 m/z [M+H]+. Example 39: 4".O-{3-[3-(3-Carboxy-1,4-dihydro-1-(2-methoxyethyl)-4-oxo-6- qulnollnyl)propylamlno]proplonyl]-azlthromycln-11.12-carbonate Using a similar procedure to that described in Example 1a, Intermediate 32c and Intermediate 4 gave the title compound as a white solid; ESMS 1134 m/z [M+H]+. Example 40: 4"-O-{3-[3-(3-Carboxy-1.4-dlhydro-1-cyclopropyl-4-oxo-6-qulnolinyl) propyiamlno]proplonyl]-azlthromycln-11.12-carbonate trlformate salt Using a similar procedure to that described in Example 1a, Intermediate 33d and Intermediate 4 gave, after chromatography, the title compound as a cream solid; ESMS 1116 m/z [M+H]+. Example 41: 4"-O-{3-[3-(3-Carboxy-1.4-dihydro-1 -cyclopropyl-4-oxo-6-quinollnyl) propylamlno]proplonyl]-6-O-methylerythromycin A dlformate 72 WO 2004/101587 PCT/EP2004/005083 Y Using a similar procedure to that described in Example 1a, Intermediate 33d and Intermediate 9 gave, after chromatography, the title compound as a tan solid; ESMS 1089 m/z [M+H]+. Example 42: 4"-O-{6-[3-(3-Carboxy-1 -ethyl-4-oxo-1.4-dlhydro-qulnolln-6-yl)- propoxy]-hexanoyll-azlthromycin 10 Hydrogenation of Intermediate 38 (106 mg, 0.095 mmol) in ethanol (15 mL) with 10 % Pd/C (14 mg) in Parr apparatus at 5 bar for 20 hours gave the title product (51 mg). MS (ES) m/z: [MH]+ 1120.68. 73 15 Example 43: 4"-O-{6-[3-(3-Carboxy-1-ethyl-4-oxo-1.4-dlhydro-quinolln-6-yl)- propoxy]-hexanoyl}-clarlthromycln WO 2004/101587 PCT/EP2004/005083 Hydrogenation of Intermediate 40 (44 mg, 0.04 mmol) in ethanol (15 mL) with 10 % Pd/C (20 mg) in Parr apparatus at 5 bar for 20 hours gave the title product (41 mg). MS (ES) m/z [MH]+1119.5. Example 44: 4"-O-{6-[3-(3-Carboxy-1 -ethyl-4-oxo-1.4-dlhydro-qulnolln-6-yl)- propoxy]-hexano yI}-11-O-methyl-azfthromycin Hydrogenation of Intermediate 42 (55 mg, 0.05 mmol) in ethanol (20 mL) with 10 % Pd/C 10 (25 mg) in Parr apparatus at 5 bar for 20 hours gave the title product (50 mg). MS (ES) m/z: [MH]+1134.51. Biological Data 15 Using a standard broth dilution method in microtitre, compounds were tested for antibacterial activity. The compounds in the above examples gave minimum inhibitory concentrations (MICs) less than 1 microgram per millilitre against erythromycin-sensitive and erythromycln-resistant strains of Streptococcus pneumoniae and Streptococcus pyogenes. 20 25 In addition, the MIC (µg/mL) of test compounds against various organisms was determined including: S. aureus Smith ATCC 13709, S. pneumoniae SP030, S. pyogenes 3565, £ faecails ATCC 29212, H. influenzae ATCC 49247, M. catarrhalls ATCC 23246. Examples 1-4, 6 and 8 have an MIC pneumoniae SP030, S. pyogenes 3565 and E faecails ATCC 29212. Examples 3,4,6 and 7 have an MIC 30 catarrhalls ATCC 23246. Examples 4-8 have an" MIC Streptococcus pneumoniae and Streptococcus pyogenes. The application of which this description and claims forms part may be used as a basis for priority in respect of any subsequent application. The claims of such subsequent 74 WO 2004/101587 PCT/EP2004/005083 application may be directed to any feature or combination of features described herein. They may take the form of product, composition, process, or use claims and may include, by way of example and without limitation, the following claims: 75 WE CLAIM: 1. Novel 14 and 15 membered ring compounds of formula (I) H3C /CH3 CH3CH2' O 14 4 H3C OCH2 (I) wherein A is a bivalent radical selected from -C(O)-, -C(0)NH-, -NHC(O)-, -N(R7)-CH2-, -CH2-N(R7)-, -CH(NR8R9)- and -C(=NR10)-; R1 is-OC(0)(CH2)dXR11; R2 is hydrogen or a hydroxyl protecting group; R3 is hydrogen, C1-4alkyl, or C3-6alkenyl optionally substituted by 9 to 10 membered fused bicyclic heteroaryl; R4 is hydroxy, C3-6alkenyloxy optionally substituted by 9 to 10 membered fused bicyclic heteroaryl, or C1-6alkoxy optionally substituted by C1-6alkoxy or -0(CH2) eNR7R12, R5 is hydroxy, or R4 and R5 taken together with the intervening atoms form a cyclic group having the following structure: 11 12 H3C wherein Y is a bivalent radical selected from -CH2-, -CH(CN)-, -0-, -N(R13)- and -CH(SR13)-; R6 is hydrogen or fluorine; R7 is hydrogen or C1-6alkyl; R8 and R9 are each independently hydrogen, C1-6alkyl, -C(=NR10)NR14R15 or -C (O)R14, or 76 R8 and R9 together form =CH(CR14R:!5)faryl, =CH(CR14R15)fheterocyclyl, =CR14R15 or =C(R14)C(O)OR14, wherein the alkyl, aryl and heterocyclyl groups are optionally substituted by up to three groups independently selected from R16; R10 is -OR17, C1-6alkyl, -(CH2)garyl, -(CH2)gheterocyclyl or -(CH2)hO(CH2)iOR7, wherein each R10 group is optionally substituted by up to three groups independently selected from R16; R11 is a heterocyclic group having the following structure: or R12 is hydrogen or C1-6alkyl; R13 is hydrogen or C1-4alkyl optionally substituted by a group selected from optionally substituted phenyl, optionally substituted 5 or 6 membered heteroaryl and optionally substituted 9 to 10 membered fused bicyclic heteroaryl; R14 and R15 are each independently hydrogen or C1-6alkyl; R16 is halogen, cyano, nitro, trifluoromethyl, azido, -C(O)R21, -C(O)OR21, - OC(O)R21, -OC(O)OR21, -NR22C(O)R23, -C(O)NR22R23, -NR22R23, hydroxy, C1_ 6alkyl, -S(O)kC1-6alkyl, C1-6alkoxy, -(CH2)maryl or-(CH2)mheteroaryl, wherein the alkoxy group is optionally substituted by up to three groups independently selected from -NR14R15, halogen and -OR14, and the aryl and heteroaryl groups are optionally substituted by up to five groups independently selected from halogen, cyano, nitro, trifluoromethyl, azido, -C(O)R24, -C(O)OR24, -OC(O)OR24, - NR25C(O)R26, -C(O)NR25R26I _NR25R26I hydroxy, C1-6alkyl and C1-6alkoxy; R17 is hydrogen, C1-6alkyl, C3-7cycloalkyl, C3-6alkenyl or a 5 or 6 membered heterocyclic group, wherein the alkyl, cycloalkyl, alkenyl and heterocyclic groups are optionally substituted by up to three substituents independently selected from optionally substituted 5 or 6 membered heterocyclic group, optionally substituted 5 or 6 membered heteroaryl, -OR27, -S(O)nR27, -NR27R28, -CONR27R28, halogen and cyano; R18 is hydrogen, -C(O)OR29, -C(O)NHR29, -C(O)CH2NO2 or-C(O)CH2SO2R7; 77 R19 is hydrogen, C1-4alkyl optionally substituted by hydroxy or C1-4alkoxy, C3_ 7cycloalkyl, or optionally substituted phenyl or benzyl; R20 is halogen, C1_4alky, C1-4thioalkyl, C1-4alkoxy, -NH2, -NH(C1-4alkyl) or - N(C1.4alkyl)2; R21 is hydrogen, C1_10alkyl, -(CH2)paryl or-(CH2)pheteroaryl; R22 and R23 are each independently hydrogen, -OR14, C1-6alkyl, -(CH2)qaryl or - (CH2)qheterocyclyl; R24 is hydrogen, C1-10alkyl, -(CH2)raryl or-(CH2)rheteroaryl; R25 and R26 are each independently hydrogen, -OR14, C1-6alkyl, -(CH2)saryl or - (CH2)sheterocyclyl; R27 and R28 are each independently hydrogen, C1-4alkyl or C1-4alkoxyC1-4alkyl; R29 is hydrogen, C1-6alkyl optionally substituted by up to three groups independently selected from halogen, cyano, C1-4alkoxy optionally substituted by phenyl or C1- 4alkoxy, -C(O)C1-6alkyl, -C(O)OC1-6alkyl, -OC(O)C1-6alkyl, -OC(O)OC1- 6alkyl, -C(O)NR32R33, -NR32R33 and phenyl optionally substituted by nitro or-C(O)OC1-6alkyl, -(CH2)wC3.7cycloalkyl, -(CH2)wheterocyclyl, -(CH2)wheteroaryl, -(CH2)waryl, C3-6alkenyl, or C3-6alkynyl; R30 is hydrogen, C1-4alkyl, C3-7cycloalkyl, optionally substituted phenyl or benzyl, acetyl or benzoyl; R31 is hydrogen or R20, or R31 and R19 are linked to form the bivalent radical - O(CH2)2-or-(CH2)t-; R32 and R33 are each independently hydrogen or C1-6alkyl optionally substituted by phenyl or-C(O)OC1-6alkyl, or R32 and R33, together with the nitrogen atom to which they are bound, form a 5 or 6 membered heterocyclic group optionally containing one additional heteroatom selected from oxygen, nitrogen and sulfur; X is -U(CH2)V-; U is a divalent radical selected from -N(R30)-, -O-, -S(O)z-, -N(R30)C(O)-, - C(O)N(R30)- and -N[C(O)R30]-; W is -C(R31)- or a nitrogen atom; d is an integer from 1 to 5; e is an integer from 2 to 4; f, g, h, m, p, q, r, s and w are each independently integers from 0 to 4; i is an integer from 1 to 6; j, k, n and z are each independently integers from 0 to 2; t is 2 or 3; v is an integer from 1 to 8; 78 or a pharmaceutically acceptable derivative thereof. 2. A compound as claimed in claim 1 wherein A is -C(O)-. 3. A compound as claimed in claim 1 or claim 2 wherein X is -N(R30)(CH2)V-- 4. A compound as claimed in any one of the preceding claims wherein d is 2. 5. A compound as claimed in any one of the preceding claims wherein R11 is a heterocyclic group of the following formula: wherein the heterocyclic is linked in the 6 or 7 position and j, R18 R19 and R20 are as defined in claim 1. 6. A compound as claimed in any one of claims 1 to 4 wherein R11 is a heterocyclic group of the following formula: wherein W is -C(R31)- where R31 and Rl9 are linked to form the bivalent radical - O(CH2)2" or -(CH2)t- and said heterocyclic is linked in the (i), (ii) or (iii) position and j, R18 and R20 are as defined in claim 1. 7. A compound as claimed in claim 1 as defined in any one of Examples 1 to 44, or a pharmaceutically acceptable derivative thereof. 8. A compound selected from: 4"-O-{3-[3-(3-carboxy-1,4-dihydro-1-ethyl-4-oxo-6-quinolinyl)propylamino]propionyl}- 6-O-methylerythromycin A; 4"-O-{3-[3-(3-carboxy-1,4-dihydro-1-ethyl-4-oxo-6-quinolinyl) propylamino]propionyl}-azithromycin-11,12-carbonate; 4"-O-{3-[3-(3-carboxy-1,4-dihydro-1-ethyl-4-oxo-6-quinolinyl) propylamino]propionyl}-6-O-methyl-11 -desoxy-11 -(R)-arnino-erythromycin A 11,12- carbamate; 79 4"-O-[3-[4-(2-carboxy-6,7-dihydro-1H,5H-pyrido[3,2,1-ij]-1-oxo-9- quinolinyl) propylamino]propionyl]-6-0-methyl erythromycin A; 4"-O-[3-[4-(3-carboxy-1-ethyl-1,4-dihydro-4-oxo-6-quinolinyl)propylamino]propionyl]- (9E)-O-({[2-(methyloxy)ethyl]oxy}methanoximino erythromycin A; 4"-O-[3-[4-(3-carboxy-1-ethyl-1,4-dihydro-4-oxo-6-quinolinyl)propylamino]propionyl]- (9E)-O-hydroximino erythromycin A; 4"-O-[3-[4-(2-carboxy-6,7-dihydro-1H,5H-pyrido[3,2,1 -ij]-1 -oxo-9- quinolinyl) propylamino]propionyl]-(9E)-O-hydroximino erythromycin A; 4"-O-{6-[3-(3-carboxy-1-ethyl-4-oxo-1,4-dihydro-quinolin-6-yl)-propoxy]-hexanoyl}- azithromycin; and 4"-O-{6-[3-(3-carboxy-1-ethyl-4-oxo-1,4-dihydro-quinolin-6-yl)-propoxy]-hexanoyl}- clarithromycin; or a pharmaceutically acceptable derivative thereof. 9. A process for the preparation of a compound as claimed in claim 1, or a pharmaceutically acceptable derivative thereof, which comprises: a) reacting a compound of formula (II) with a suitable activated derivative of the acid (III), wherein Xa and R11a are X and R11 as defined in claim 1 or groups convertible to X and R11; b) reacting a compound of formula (IV) 80 (IV) with a compound of formula XaR11a (V), wherein R11a is R11 as defined in claim 1 or a group convertible to R11 and Xa is -U(CH2)V- or a group convertible to - U(CH2)V- in which U is a group selected from -N(R30)- and -S-, and L is suitable leaving group, to produce a compound of formula (I) wherein U is a group selected from -N(R30)- and -S-; or c) reacting a compound of formula (VII), with a compound of formula XaR11a (V), wherein R11a is R11 as defined in claim 1 or a group convertible to R11 and Xa is - U(CH2)V- or a group convertible to -U(CH2)V- in which U is a group selected from - N(R30)- and -S-, to produce a compound of formula (I) wherein d is 2 and U is a group selected from -N(R30)- and -S-, and thereafter, if required, subjecting the resulting compound to one or more of the following operations: i) removal of the protecting group R2 ii) conversion of XaR11a to XR11, and iii) conversion of the resultant compound of formula (I) into a pharmaceutically acceptable derivative thereof. 81 10. A compound as claimed in any one of claims 1 to 8, or a pharmaceutically acceptable derivative thereof, for therapy. 11. A pharmaceutical composition comprising at least one compound as claimed in any one of claims 1 to 8, or a pharmaceutically acceptable derivative thereof, in association with a pharmaceutically acceptable excipient, diluent and/or carrier. 12. A pharmaceutical composition as claimed in claim 11 in the manufacture of a medicament for the treatment or prophylaxis of systemic or topical microbial infections in a human or animal body. 13. A pharmaceutical composition as claimed in claim 11 for the treatment or prophylaxis of systemic or topical microbial infections in a human or animal body. 14. A compound of formula (IA): H,C OCH, (IA) wherein A is a bivalent radical selected from -C(O)-, -C(O)NH-, -NHC(O)-, -N(R7)-CH2-, -CH2-N(R7)-, -CH(NR8R9)- and -C(=NR10)-; R1 is-OC(O)(CH2)dXR11; R2 is hydrogen or a hydroxyl protecting group; R3 is hydrogen, C1-4alkyl, or C3-6alkenyl optionally substituted by 9 to 10 membered fused bicyclic heteroaryl; R4 is hydroxy, C3-6alkenyloxy optionally substituted by 9 to 10 membered fused bicyclic heteroaryl, or C1-6alkoxy optionally substituted by C1-6alkoxy or -O(CH2) eNR7R12, R5 is hydroxy, or R4 and R5 taken together with the intervening atoms form a cyclic group having the following structure: 82 wherein Y is a bivalent radical selected from -CH2-, -CH(CN)-, -O-, -N(R13)- and - CH(SR13)-; R6 is hydrogen or fluorine; R7 is hydrogen or C1-6alkyl; R8 and R9 are each independently hydrogen, C1-6alkyl, -C(=NR10)NR14R15 or - C(O)R14, or R8 and R9 together form =CH(CR14R15)faryl, =CH(CR14R15)fheterocyclyl, =CR14R15 or =C(R14)C(O)OR14, wherein the alkyl, aryl and heterocyclyl groups are optionally substituted by up to three groups independently selected from R16; R10 is -OR17, C1-6alkyl, -(CH2)garyl, -(CH2)gheterocyclyl or -(CH2)hO(CH2)iOR7, wherein each R10 group is optionally substituted by up to three groups independently selected from R16; R11 is a heterocyclic group having the following structure: or R12 is hydrogen or C1-6alkyl; R13 is hydrogen or C1-4alkyl optionally substituted by a group selected from optionally substituted phenyl, optionally substituted 5 or 6 membered heteroaryl and optionally substituted 9 to 10 membered fused bicyclic heteroaryl; R14 and R15 are each independently hydrogen or C1-6alkyl; R16 is halogen, cyano, nitro, trifluoromethyl, azido, -C(O)R21, -C(O)OR21, - OC(O)R21, -OC(O)OR21, -NR22C(O)R23, -C(O)NR22R23, -NR22R23, hydroxy, C1- 6alkyl, -S(O)kC-1-6alkyl, C1-6alkoxy, -(CH2)maryl or-(CH2)mheteroaryl, wherein the alkoxy group is optionally substituted by up to three groups independently selected from -NR14R15, halogen and -OR14, and the aryl and heteroaryl groups are 83 optionally substituted by up to five groups independently selected from halogen, ■cyano, nitro, trifluoromethyl, azido, -C(O)R24, -C(O)OR24, -OC(O)OR24, - NR25C(O)R26, -C(O)NR25R26, -NR25R26, hydroxy, C1-6alkyl and C1-6alkoxy; R17 is hydrogen, C1-6alkyl, C3-7cycloalkyl, C3-6alkenyl or a 5 or 6 membered heterocyclic group, wherein the alkyl, cycloalkyl, alkenyl and heterocyclic groups are optionally substituted by up to three substituents independently selected from optionally substituted 5 or 6 membered heterocyclic group, optionally substituted 5 or 6 membered heteroaryl, -OR27, -S(O)nR27, -NR27R28, -CONR27R28, halogen and cyano; R18 is hydrogen, -C(O)OR29, -C(O)NHR29 or-C(O)CH2NO2; R19 is hydrogen, C1-4alkyl optionally substituted by hydroxy or C1-4alkoxy, C3- 7cycloalkyl, or optionally substituted phenyl or benzyl; R20 is halogen, C1-4alkyl, C1-4thioalkyl, C1-4alkoxy, -NH2, -NH(C1-4alkyl) or - N(C1.4alkyl)2; R21 is hydrogen, C1-10alkyl, -(CH2)paryl or-(CH2)pheteroaryl; R22 and R23 are each independently hydrogen, -OR14, C1-6alkyl, -(CH2)qaryl or - (CH2)qheterocyclyl; R24 is hydrogen, C1-10alkyl, -(CH2)raryl or-(CH2)rheteroaryl; R25 and R26 are each independently hydrogen, -OR14, C1-6alkyl, -(CH2)saryl or - (CH2)sheterocyclyl; R27 and R28 are each independently hydrogen, C1-4alkyl or C1-4alkoxyC1-4alkyl; R29 is hydrogen, C1-6alkyl optionally substituted by up to three groups independently selected from halogen, C1-4alkoxy, -OC(O)C1-6alkyl, -OC(O)OC1- 6alkyl, -C(O)NR32R33 and -NR32R33, -(CH2)wC3-7cycloalkyl, C3-6alkenyl or C3. 6alkynyl; R30 is hydrogen, C1-4alkyl, C3-7cycloalkyl, optionally substituted phenyl or benzyl, acetyl or benzoyl; R31 is hydrogen or R20 or R31 and R19 are linked to form the bivalent radical - O(CH2)2-or-(CH2)t-; R32 and R33 are each independently hydrogen or C1-6alkyl optionally substituted by -C(O)OC1-6alkyl, or R32 and R33, together with the nitrogen atom to which they are bound, form a 5 or 6 membered heterocyclic group optionally containing one additional heteroatom selected from oxygen, nitrogen and sulfur; X is -U(CH2)V-; U is a divalent radical selected from -N(R30)-, -O-, -S(O)z-, -N(R30)C(O)-, - C(O)N(R30)- and -N[C(O)R30]-; W is -C(R31)- or a nitrogen atom; d is an integer from 1 to 5; e is an integer from 2 to 4; f, g, h, m, p, q, r, s and w are each independently integers from 0 to 4; i is an integer from 1 to 6; 84 j, k, n and z are each independently integers from 0 to 2; t is 2 or 3; v is an integer from 2 to 8; or a pharmaceutically acceptable derivative thereof. 15. A compound of formula (IB) CH3CH2 O 14 i- H3C OCH3 (IB) wherein A is a bivalent radical selected from -C(O)-, -C(O)NH-, -NHC(O)-, -N(R7)-CH2-, - CH2-N(R7)-, -CH(NR8R9)- and -C(=NR10)-; R1 is-OC(O)(CH2)dXR11; R2i is hydrogen or a hydroxyl protecting group; R3 is hydrogen, C1-4alkyl, or C3-6alkenyl optionally substituted by 9 to 10 membered fused bicyclic heteroaryl; R4 is hydroxy, C3-6alkenyloxy optionally substituted by 9 to 10 membered fused bicyclic heteroaryl, or C1-6alkoxy optionally substituted by C1-6alkoxy or - O(CH2)eNR7R12, R5 is hydroxy, or R4 and R5 taken together with the intervening atoms form a cyclic group having the following structure: wherein Y is a bivalent radical selected from -CH2-, -CH(CN)-, -O-, -N(R13)- and - CH(SR13)-; R6 is hydrogen or fluorine; R7 is hydrogen or C1-6alkyl; 85 R8 and R9 are each independently hydrogen, C1-6alkyl, -C(=NR10)NR14R15 or - C(O)R14, or R8 and R9 together form =CH(CR14R15)faryl, =CH(CR14R15)fheterocyclyl, =CR14R15 or =C(R14)C(O)OR14, wherein the alkyl, aryl and heterocyclyl groups are optionally substituted by up to three groups independently selected from R16; R10 is -OR17, C1-6alkyl, -(CH2)garyl, -(CH2)gheterocyclyl or -(CH2)hO(CH2)iOR7, wherein each R10 group is optionally substituted by up to three groups independently selected from R16; R11 is a heterocyclic group having the following structure: R12 is hydrogen or C1-6alkyl; R13 IS hydrogen or C1-4alkyl substituted by a group selected from optionally substituted phenyl, optionally substituted 5 or 6 membered heteroaryl and optionally substituted 9 to 10 membered fused bicyclic heteroaryl; R14 and R15 are each independently hydrogen or C1-6alkyl; R16 is halogen, cyano, nitro, trifluoromethyl, azido, -C(O)R21, -C(O)OR21, - OC(O)R21, -OC(O)OR21, -NR22C(O)R23, -C(O)NR22R23, -NR22R23, hydroxy, C1- 6alkyl, -S(O)kC1-6alkyl, C1-6alkoxy, -(CH2)maryl or -(CH2)mheteroaryl, wherein the alkoxy group is optionally substituted by up to three groups independently selected from -NR14R15 halogen and -OR14, and the aryl and heteroaryl groups are optionally substituted by up to five groups independently selected from halogen, cyano, nitro, trifluoromethyl, azido, -C(O)R24 -C(O)OR24, -OC(O)OR24, - NR25C(O)R26, -C(O)NR25R26, -NR25R26, hydroxy, C1-6alkyl and C1-6alkoxy; R17 is hydrogen, C1-6alkyl, C3-7cycloalkyl, C3-6alkenyl or a 5 or 6 membered heterocyclic group, wherein the alkyl, cycloalkyl, alkenyl and heterocyclic groups are optionally substituted by up to three substituents independently selected from optionally substituted 5 or 6 membered heterocyclic group, optionally substituted 5 or 6 membered heteroaryl, -OR27, -S(O)nR27, -NR27R28, -CONR27R28 halogen and cyano; 86 R18 is hydrogen, -C(O)OR29, -C(O)NHR29 or -C(O)CH2N02; R19 is hydrogen, C1-4alkyl optionally substituted by hydroxy or C1-4alkoxy, C3- 7cycloalkyl, or optionally substituted phenyl or benzyl; R20 is halogen, C1-4alkyl, C1-4thioalkyl, C1-4alkoxy, -NH2, -NH(C1-4alkyr) or - N(C1-4alkyl)2; R21 is hydrogen, C1-10alkyl, -(CH2)paryl or-(CH2)pheteroaryl; R22 and R23 are each independently hydrogen, -OR114, C1-6alkyl, -(CH2)qaryl or - (CH2)qheterocyclyl; R24 is hydrogen, C1-10alkyl, -(CH2)raryl or -(CH2)rheteroaryl; R25 and R26 are each independently hydrogen, -OR14, C1-6alkyl, -(CH2)saryl or - (CH2)sheterocyclyl; R27 and R28 are each independently hydrogen, C1-4alkyl or C1-4alkoxyC1-4alkyl; R29 IS hydrogen or C1-6alkyl optionally substituted by up to three groups independently selected from halogen, C1-4alkoxy, -OC(O)C1-6alkyl and - OC(O)OC1-6alkyl; R30 IS hydrogen, C1-4alkyl, C3-7cycloalkyl, optionally substituted phenyl or benzyl, acetyl or benzoyl; R31 IS hydrogen or R20 or R31 and R19 are linked to form the bivalent radical - O(CH2)2-or-(CH2)t-; X is -U(CH2)V-; U is a divalent radical selected from -N(R30)-, -O-, -S(O)z-, -N(R30)C(O)-, - C(O)N(R30)- and -N[C(O)R30]-; W is -C(R31)- or a nitrogen atom; d is an integer from 1 to 5; e is an integer from 2 to 4; f, g, h, m, p, q, r and s are each independently integers from 0 to 4; i is an integer from 1 to 6; j, k, n and z are each independently integers from 0 to 2; t is 2 or 3; v is an integer from 2 to 8; or a pharmaceutically acceptable derivative thereof. 87 The present invention relates to 14- or 15-mem- bered macrolides substituted at the 4" position of formula (I) and pharmaceutically acceptable derivatives thereof, to processes for their preparation and their use in therapy or prophylaxis of systemic or topical microbial infections in a human or animal body.

Documents:

2192-kolnp-2005-granted-abstract.pdf

2192-kolnp-2005-granted-assignment.pdf

2192-kolnp-2005-granted-claims.pdf

2192-kolnp-2005-granted-correspondence.pdf

2192-kolnp-2005-granted-description (complete).pdf

2192-kolnp-2005-granted-examination report.pdf

2192-kolnp-2005-granted-form 1.pdf

2192-kolnp-2005-granted-form 18.pdf

2192-kolnp-2005-granted-form 3.pdf

2192-kolnp-2005-granted-form 5.pdf

2192-kolnp-2005-granted-gpa.pdf

2192-kolnp-2005-granted-reply to examination report.pdf

2192-kolnp-2005-granted-specification.pdf