| Title of Invention |
A FUSION PROTEIN AND A METHOD FOR DETERMINING THE BINDING OF COMPOUNDS TO THE LEPTIN RECEPTOR |
| Abstract |
The present invention relates to a fusion protein, characterized in that it is composed of a leptin receptor having a short intracellular domain wherein the leptin receptor is an isoform comprising a Boxl intracellular domain, free of a Box 3 intracellular domain or of a soluble form of such a receptor containing the leptin- binding site, or of a substantial portion of such a leptin receptor, and of an energy donor or energy acceptor protein, or of a substantial and active portion of an energy donor or energy acceptor protein. The invention also relates to a method for determining the binding of compounds to the leptin receptor, comprising the steps consisting in: -bringing said compound into contact with an energy donor fusion protein as herein described and an energy acceptor fusion protein as herein described, or cells, or fragments or lysates or membranes of cells comprising such a protein, and optionally an appropriate enzyme substrate, and -measuring the energy transfer. |
Documents:
1859-chenp-2004 abstract.pdf
1859-chenp-2004 claims.pdf
1859-chenp-2004 correspondence others.pdf
1859-chenp-2004 correspondence po.pdf
1859-chenp-2004 description (complete).pdf
1859-chenp-2004 drawings.pdf
1859-chenp-2004 form 1.pdf
1859-chenp-2004 form 18.pdf
1859-chenp-2004 form 3.pdf
1859-chenp-2004 form 5.pdf
1859-chenp-2004 pct.pdf
1859-chenp-2004 petition.pdf
1859-chenp-2004 power of attorney.pdf