Indian Patents. 233191:"DEVICES FOR COMPONENT REMOVAL DURING BLOOD COLLECTION, AND USES THEREOF"

Title of Invention	"DEVICES FOR COMPONENT REMOVAL DURING BLOOD COLLECTION, AND USES THEREOF"
Abstract	The invention relates to a device (120) for collecting blood whereby certain target components are isolated or removed from the blood sample at the time of collecting the blood. FIG. 1

Full Text	DEVICE FOR COLLECTING BLOOD, FOR COMPONENT REMOVAL DURING BLOOD COLLECTION Background of the Invention Field of Invention [0001] The present invention relates to devices for collecting blood, for component removal during blood collection, whereby certain target components are isolated or removed from the blood sample at the time of collecting the blood, as well as methods of using such devices. Background of the Invention [0002] Today with the growth of proteomics, large-scale isolation and identification of proteins is becoming the focus of research, drug development and diagnostics. Protein separation methods are well-established in the molecular and biochemical arts; however, with the growth of proteomics, new technologies have arisen which require different, more stringent protein separation techniques. For example, exploring the plasma proteome requires the separation and identification of low abundance proteins and the determination of their characteristics using electrophoresis in a 2-dimensional (2D) gel system. However, plasma proteins or other components present in high concentrations, for example albumin, can hinder the separation and obscure the identification of these lower abundance proteins and, at times, make it difficult or impossible to determine their identity on 2D gels or by any other downstream testing of blood samples. [0003] Currently, many different methods exist to remove albumin and other high abundance components from blood samples; however, most of these clean-up methods are not specific enough and may actually remove many of the desirable proteins or components. Current albumin clean-up methods, for example, require transferring the sample into another container, thus requiring additional handling steps, and increasing the risk of processing errors, sample contamination, and extra risk of operator exposure to possibly infectious blood components. Furthermore, current methods do not use any kind of specifically directed affinity molecule targeting the target components in the clean-up process, and may thus remove components of interest. Accordingly, there is a need for blood sample collection devices, methods of using such devices, and processes that eliminate the risk of error and contamination, while also providing specific isolation of target components. Summary of the Invention 100041 The present invention relates to a device for collecting blood comprising a reservoir and a f f i n i t y molecules, wherein the affinity molecules arc exposed to blood during the time of blood collection, [0005] The present invention also relates to a method of collecting blood comprising exposing the blood to affinity molecules during the time of blood collection, Brief Description of the Drawings (0006) FIG. 1 depicts one of the devices of the present invention. J0007] FIG. ." is a longitudinal sectional view of the device of FIG. 1. \|OOU8\| FIG. •• is a longitudinal sectional view of the device of FIG. 1, illustrating fluid Jcin try into the device. [0009] FIG. 'I illustrates the device of FIG. 1 during centrifugation, with the subsequent release of the separator. [0010\| FIG. 5 also illustrates the device of FIG. 1 during centrifligation, with the subsequent re least of the separator. \|00l 1] FIG. t) illustrates the device of FIG. 1 after centrirugation, showing the separation of (he l i q u i d sample Into higher and lower specific gravities. [0012] FIG. 7 depicts another device of the present invention. [0013] FIG. 8 is a longitudinal sectional view of the device of FIG. 7. [0014] FIG. 9 is a longitudinal sectional view of the device of FIG. 7, illustrating fluid delivery into the device. [0015] FIG. 10 illustrates the device of FIG. 7 during centrifugation, with subsequent release of the separator. [\|0()16\| FIG. 1 1 also illustrates the device of FIG. 7 during centrifugation, with subsequent release of the separator, [0017] FIG. 12 illustrates the device of FIG. 7 after centrifugation, showing separation of the liquid sample into higher and lower specific gravities. [0018\| FIG. 13 depicts another device of the present invention. [0019] FIG. 14 depicts another device of the present invention, wherein antibody-coated beads are placed within a syringe assembly. \|0020] FIG. ! 5 depicts another device of the present invention, wherein antibody-coated tubing is used for in-line component removal. Detailed Description of the Invention [0021) 'Hie present invention relates to a device for collecting blood comprising a reservoir and a f f i n i ty molecules, wherein the affinity molecules are exposed to the blood during the nine of collecting the blood. [0022) The present invention also relates to a method of collecting blood comprising exposing the blood to affinity molecules during the time of said collection. The affinity molecules will bind target molecules present in the blood. As used herein, "target molecules" or "targets" is used to mean a component that may or may not be present in the actual sample of blood, but will bind to the affinity molecules if present.. The target may be "desired" in that Ihe moltcule targeted by the o Trinity molecule is of interest for further processing or assaying. Alternatively, the target may be "undesired" in that the target may be considered an impurity, or may be unnecessar' or unwanted in further processing or assaying steps. J0023\| As used herein, "blood" is intended to mean whole blood, or the separate components of whole blood, from any animal. Separate components of blood include, but are not limited to, plasma and serum, the definitions of which are used herein as these terms are used in the art. For example, plasma is the component of whole blood that is substantially free of cells, including plaielels. Serum is plasma in which fibrinogen and other clotting factors have been removed. Thus, "blood," as used herein, can include whole blood, plasma, serum, the cellular portion of blood and even the protein portion of whole blood. Furthermore, for the purposes of this invention, components of blood can be isolated, such as the isolation of protein or other molecules from blood such as an isolate of fibrinogen, or can be a mixture of molecules, such as plasma or serum. For example, as used in the current context, albumin is considered a component of blood. As used herein, "component" does not include the cellular portion of blood, whereas, "cellular component" includes cellular portions. Cellular components may or may not include, and are not limited to, hematopoetic cells, myeioid stem cells, erythrocytes, neutrophils, basophils. eosinophils, megakaryocytes, platelets, monocytcs, mast ceils, lymphoid cells, including all T-cells and B-cells, normal or diseased cells from any body tissue. [0024\| Furthermore, "collecting" is used to mean extraction of the blood from its normal environment, whether or not the blood is returned to the individual from which it was extracted, or placed into a different individual, such as during a transfusion. As used herein, the terms "individual," "subject" and "patient" are used interchangeably and are used to mean any animal, preferably a mammal, more preferably a human or non-human primate. [0025] The device of the present invention comprises a reservoir. The reservoir can be any device, open ur closed to the environment, that can hold any amount of blood, at least temporarily. Of c reservoir can also be capable of being punctured and resealed. Examples of reservoirs of the current invention include, but are not limited to, evacuated tubes, non-evacuated tubes, petri dishes, microtiter plates, syringe barrels, needles, machines that can process or analyze blood, blood collect ion systems such as blood collection bags, such as the type used when donating blood, iiiid even plastic tubing. [0026] The blood need not be stationary inside the reservoir, but can be sedentary, or moving or flowing, la one embodiment, the reservoir comprises a density gradient barrier wherein the sample collected within the reservoir is separated into higher and lower densities during centrifugation. Any type ofblood collection device or system may be used as a reservoir. Suitable devices and systems that can act as reservoirs include, but are not limited to, blood tubes manufactured by, for example, Becton Dickinson and Company (BD) including, but not limited to, Serum Clot Activator Tubes, Serum Gel Clot Activator Tubes, Lithium Heparin Tubes, Sodium Fluoride Tubes, Sodium Citrate Tubes, ESR Citrate Tubes and Trace Mettal Tubes. Additionally, capillary blood collection systems produced by any manufacturer including, but not limited to, Microvette™ systems can act as reservoirs. Examples of some types of reservoirs contemplated for use in the present invention are contained in U.S. Patent No. 6,479,298, the entirety of which is incorporated herein by reference. [0027] In one embodiment, the reservoir further comprises an insert. As used herein, the term "insert" means a material that is completely encapsulated by the reservoir, such as a solid dipstick, mechanical density partition element, or a paddle within an evacuated tube. The term "insert" can also refer to material that is only partially within the reservoir. The inserts can be a solid object, such as a paddle, or the inserts can be a liquid, a gel or a gas. Other examples ot inserts include, but are not limited to, beads, particles and microparticlcs. Examples of beads include, but are not limited to, paramagnetic beads that can be manipulated by a magnetic field. The inserts can be any shape, provided that at least a portion of the insert is located within the reservoir. Examples of the shapes of inserts include, but are not limited to, rectangular, circular, oval, straight rod and helical-shaped rod. 10028] In another embodiment, the interior walls of the reservoir can be smooth or ridged. Providing a reservoir with ridges serves to increase the interior surface area of the reservoir. In yet another embodiment, the inserts are also ridged to increase their respective surface areas. In still another embodiment, both the insert and the interior walls of the reservoir arc ridged to provide even more surface area. The purpose of increasing the surface area in any of the embodiments of the present invention is to increase the amount of surface-immobilized affinity molecules that can come into contact with the blood at the time of its collection. [0029] In addition to the reservoir, the device of the present invention also comprises one or more affinity molecules. As used herein, "affinity molecules" can be any type of molecule that, possesses an affinity towards single or multiple components of the blood. The affinity can be a specific affinity in that the affinity molecule has an affinity for a focused subset of components, such as an antibody specific for fibrinogen. The affinity of the affinity molecules can also be less specific in nature, such as a protein that binds the general class immunoglobulins, for example, protein A. Even further, the affinity molecules as contemplated by the present invention can exhibit an even broader affinity such that the affinity molecules can possess an affinity for a mixture of different classes of proteins. [0030] Examples of affinity molecules include, but are not limited to, antibodies, antibody fragments, enzymes, fragments of enzymes, enzyme substrates, fragments of enzyme substrates, nucleotides, oligonucleotides, polynucleotides, receptors, fragments of receptors, ligands. fragments of enzymes, other proteins, amino acids, peptides, polypeptides, oligopeptides, saccharides, disaccharides, polysaccharides, glycoproteins, proteoglycans, natural and synthetic polymers, aptamers, and nanobodics. [n one embodiment, the affinity molecules arc antibodies, or fragments of antibodies, such as, but not limited to, Fab fragments, FiulV'b fragments, and scFv fragments. The antibodies may be polyclonal, monoclonal, chimeric, synthetic or naturally occurring. [0031] In another embodiment, the affinity molecules of the present invention bind a component within the blood. Preferably, the affinity molecules bind a blood protein. Examples of proteins that the affinity molecules will bind include, but are not limited to, fibrinogen, albumin, immunoglobulins including, but not limited to, alpha-, beta- and gamma-globulins, proteases such as chymotrypsin and thrombin, and protease inhibitors such as antichymotrypsin, untithrombin, macroglobulins, intcr-alpha-trypsin inhibitor, Cl i n h i b i t o r , plusmin inhibitor, hcparin cofactor II, apoprotein A and fibronectin. [0032) In OIK: embodiment, the affinity molecule or set of affinity molecules can bind to more tlv.in one nudes i red component at the same lime, resulting in more than one undcsired component ixmg removed from the sample. In another embodiment, the affinity molecule or set ol .'affinity molecules can bind lo more than one desired component at the same time, resulting in more than one desired component being isolated. In another embodiment, more than one affinity molecule or set of affinity molecules can be employed, with each affinity molecule or set of molecules binding different undesired components, resulting in more than one uiHiesiruJ component being removed from the lampk. Of course, the invention encompasses ut.iliv.ing one affinity molecule or set of affinity molecules that can bind only one undcsired component. Optionally, the invention encompasses utilizing one affinity molecule or a set of affinity molecules that can enrich the sample by removing all but a few desired components from the original specimen. As used herein, the phrase "a set of affinity molecules" is used to mean a plurality of affinity molecules that possess the identical type of affinity, in both specificity and strength. As used herein, "affinity molecules" is used to mean one or more affinity molecules and one or more sets of affinity molecules. In one embodiment, the invention encompasses a reservoir and a single device, comprising an affinity molecule or a set of affinity molecules. In another embodiment, the invention also encompasses a single device, comprising a mixture of more than one distinct affinity molecules or sets of molecules. In yet another example, the invention also comprises multiple devices, with each device comprising a distinct affinity molecule or set of affinity molecules directed towards, for example, itnmunoglobulins and clotting cactors. In still another embodiment, the invention also comprises multiple devices, witu each device comprising a mixture of more than one distinct affinity molecules or sets of affinity molecules. In still another embodiment, the invention also comprises a kit of multiple devices, with each device comprising a mixture oi more than one distinct affinity molecules or sets of affinity molecules. [0033] The affinity molecules of the present invention can be present in soluble or immobilized rbrm. If. for example, the affinity molecules arc in soluble form, they may be present in the reservoir in liquid or lyophilized form. The blood would then be added to the solubili/.ed ailinitv molecules, which could then be pulled oat of the sample or fractionated from the sample depending on the specific user needs for the final sample. For example, the aifinity molecules can be bound or attached to inert substances such as, for example, glass, polypropylene, nylon, nitrocellulose, polymcthacrylate, polystyrene, polyvinylchloride, styrenebutadiene copolymer, styrene-acrylatc copolymer, latex, chemically modified plastic, rubber, red blood cells, -_i polymeric material or biological cells. According to the present invention, examples of the surfaces on which the inert substances can be found include, but are not limited to, any portion of the reservoir, the sides and/or bottoms of tubes, the sides and/or bottom of culture dishes, aay type of insert such as a paddle, dipstick, gel, beads, particles, microparticles, and the I ning of tubing. The affinity molecules can be attached to the surfaces by any known method including, but not limited to. covalent and non-covalenl oTtachnitiit or coupling, such as at! iching the affinity molecules directly to the inert surfaces or attaching the affinity molecules o derivatized and/or coated surfaces. \|0034\| In one embodiment, microp irticles such as beads are used in the present invention, with the beads comprising scpharo.ie or polystyrene. In another embodiment, the beads may comprise magnetic particles, such as magnetic, paramagnetic, and superparamagnetic beads produced by Dynal AS (Oslo, Norway) and sold as DYNABEADS™. If magnetic beads are used, regard le >s o! the manufacturer, a magnet could be used to manipulate the movement of the proteins bound to the beads. The magnet can further be used to collect or separate the beads from the rest of the blood sample. [0035] In another embodiment of the present of the invention, a density separator gradient may be introduced to further separate density phases of a collected sample. Such density gradients may be chosen from the group consisting of a thixotropic gel and a mechanical separator. Additionally, beads or microparticles may be chosen such that their density is greater than the density separator. For instance, if the density of the separator is about 1.02 to about 1.08 g/cin', the density of the beads may be chosen such that all or a portion of the beads have a density greater than about 1.08 g/cnr\ The higher density beads should come to rest at the bottom of the container during ccntrifugation, thus moving a portion of the bound targets below the density separator. When the targeted molecules are highly abundant, removal of the beads or microparticles will thus enrich the sample remaining in the reservoir. In another embodiment of the present invention, beads may be chosen such that their density is smaller than the density of a density separator. For instance, if the density of the separator is about i .02 to about 1.08 g/cnr , the density of the beads may be chosen such that all or a portion ot the beads have a density lower than about 1.02 g/cm3. The lower density beads should iloat to the top of the sample, thus moving a portion of the bound targeted proteins above the separator. When the targeted molecules are highly abundant, removal of the beads or microparticles will thus enrich the sample remaining in the reservoir. [0036] The ih.ixotropic gel separator may be applied to the inner surface the reservoir. Altermnely, ;i mechanical separator may be inserted within the tube to separate fluids. Once the blood collection tube is subjected to centrifugal force, the separator will then move up or down the tube as it comes into a density equilibrium with the blood sample, thus acting to set up a density gradient for the blood. In conjunction with the separator, the beads, if used in Hie present invention, can also act within and help establish a density gradient to allow ease uf separation of tht- various components of the blood. [0037] The present invention may be embodied in other specific forms and is not limited to any specific embodiments described in detail, which are merely exemplary. Various other modifications will be apparent to and readily made by those skilled in the art without departing from the scope and spirit of the invention. The scope of the invention will be measured by the appended claims and their equivalents. [0038] One embodiment of the present invention is illustrated in FIGS. 1-6, wherein assembly 120 comprises a reservoir 130 (here, depicted as a tube), a closure 150 and a separator 170. [0039] Reservoir 130 has an open end 132 that includes a top edge (not shown), a closed end 134 and a sidevvall 136 extending between the open end and the closed end. Sidewall 136 has an outer surface 138 and an inner surface 140. Reservoir 130 defines a receptacle with a central axis "A". Assembly 120 includes affinity molecules 131 located on inner surface 140 of sidewall 136. \|0040\| Closure 150 is disposed to fit over open end 132 of reservoir 130. Closure 150 comprises an annular upper portion 152, which extends over the top edge of sidewall 136, and a lower annular portion or skirt 154 of lesser diameter than the annular upper portion 152, which extends into and forms an interference fit with inner surface 140 of sidewall 136 for maintaining closure 150 in place in open end 132. [0041] Annular upper portion 152 includes a top surface area 156, sidewall 158 that converges from surface area 156 towards upper well area 160. Lower annular skirt portion 154 defines a lower well 162, an inner wall surface 164, an outer wall surface 166 and a bottom surface 168, Well area 160 and lower well area 162 define a thin diaphragm or selfsealing septum through which a needle may be inserted. The self-sealing septum material allows penetration by a piercing element such as a needle and then rcseals when the piercing element is withdrawn. [0042] An annular ledge or abutment 157 separates annular upper portion 152 and lower annular portion 154, [0043] Preferably, closure 150 is made of natural rubber elastomer, synthetic thermoplastic and ihermoset elastomeric materials. Preferably, the closure is made of a resilient elastomeric material whereby the septum is self-sealing. [0044] As shown in FIG. 3, a liquid sample A is delivered to the tube by a needle that penetrates closure 150 in upper well area 160. For purposes of illustration only, the liquid sample is blood. The liquid sample is delivered into the passageway of separator 170 so that the liquid sample is introduced between closed end 134 of reservoir 130 and separator 170, whereby the outer surface of all components of separator 170 are substantially free of any contact with the fluid sample. [0045] As shown in FIGS. 4 and 5, when assembly 120 is subjected to centriftigation or axial centrifugation force, seal body 191 of separator 170 deflects, thereby reducing its diameter and eliminating its interference lit with the inner wall of the reservoir. Separator 170 releases from the inner wail of the reservoir such that separator 170 descends towards closed end 134 of reservoir 1 30. This also opens up a path 300 between the reservoir and the separator, permitting the tlow of the low-density component B of the fluid upwardly past the separator as the separator migrates down the reservoir. The low-density component inside the passageway i 99 of the separator will migrate downwardly and upwardly past the separator. Thus, separator 170 is permitted to sink into the fluid sample. (0040] As shown in FIG. 6, after ccntrifugation is terminated, the absence of the centrifugal load will cause seal body 191 to resiliency return toward an underformed condition and lightly seal with the inner wall of the tube. Thus, separator 170 serves as a divider between lower specific gravity portion B and higher specific gravity portion C of the liquid sample. \|0047\| FIGS. 7-12 represent an alternative embodiment of the present invention. The embodiment of FIGS. 7-12 operates in the same manner as the embodiment of FIGS. 1-6, but contains beads 135' w i th a f f i n i ty molecules 131' thereon, as well as affinity molecules 131' 1'ieutxd on inner surface 140' of sidcwall 136'. Elements common lo the embodiment of I ' I G S . l-o and FIGS. 7-12 are numbered identically, with those of the latter embodiment denoted as "prime': ('). [0048] FIG. 13 shows the interaction of the device of FIGS. 1-6 with a holder 42. Holder 42 has a cannula 44 with a venous entry end 48 and a non-patient end 46 such that when :i>sembly 120 i? inserted into holder 42, the self-scaling septum on closure 150 is pierced by non-puii'_ nt tud 4o, thereby starting the process of collecting blood into reservoir 130. 10049] In one embodiment, the reservoir is compatible with the numerous additives used in the art such us citrates, silicone. silicates, ethylenediaminctetraacctic acid (EDTA) and the like. The additives are used, for example, to condition the sample either to facilitate or retard clotting or to preserve the sample for a particular analysis. It is within the purview of the present invention that one or more additives may be used. [0050] The affinity molecules themselves may or may not be modified to allow ease of separation. For example, the affinity molecules may be biotinylated, and streptavidin could be used to remove the biotinylated affinity molecules after having bound the target component. Thus, a component can be removed from a sample first by exposing the affinity molecules to the sample at the time of collection and subsequently using biotinylated anti-affinity molecule antibodies. The biotinylated antibodies would then be captured using, for example, avidin, streptavidin, or Neutravidin™, resulting in a sampL substantially depleted of the targeted component. [0051] In one embodiment, the affinity molecules are found within the reservoir. For example, the affinity molecules may be bound to beads, which are found within the reservoir. Thus, tlic invention can comprise an evacuated blood collection device with, for example, antibody-coated beads added to the tube. The tube containing the beads would be used, for example, during venipuncture blood collection procedures in human.s and/or animals. In a similar embodiment, the invention could also be, tor example, non-evacuated microcollection containers containing antibody-coated beads. The foregoing embodiment can be modified, for example, by substituting the beads for any inert substance or surface, whether or not the inert substance or surface is listed herein. [0052] In another embodiment, the affinity molecules may be external to the reservoir. For example, the affinity molecules may be bound to beads that are external to the reservoir and m;i'-' comprise, for example, an affinity column packed with affinity beads. Thus, the invention can also comprise a reservoir for collecting the blood and one or more affinity columns linked, in sequence, before or after and optionally integrated to the reservoir. The foregoing embodiment can be modified, lor example, by substituting the beads for any portion of the reservoir, the sides tnd/or bottoms of tubes, particles, microparticles, the sides and/or bottom of culture dishes and the lining of tubing. [0053] Indeed, lilt, loregoing embo liments can be modified, for example, by substituting the beads for any men substance or sur ace, whether or not the inert substance or surface is listed herein. Further, all of the cmbodim ;nts described herein can also be modified by substituting antibodies, for example, with any t} pe of affinity molecule such as antibody fragments, enzymes, fragments of enzymes, crzyme substrates, fragments of enzyme substrates, nucleotides, oligonucleotides, polynucleotides, receptors, fragments of receptors, ligands, fragments of enzymes, other proteins, amino acids, pcptides, polypeptides, oligopeptides, saccharides, di.saccharides, polysaccharides, glycoproteins, proteoglycans, and natural and synthetic polymer*-.. Further, all of the embodiments described herein can be modified by substituting protein for any undesirable component of blood. [0054] The present invention provides distinct advantages over current methods, these advantages being achieved by exposing the affinity molecules to the blood during and/or immediately alter the blood collection process. As used herein, "during the time of collection" is used to mean that the affinity molecules are exposed to the blood during the blood collection process, The exposure of the blood to the affinity molecules need not be briefer instantaneous in time, so long as the exposure of the biood to the affinity molecules occurs prior to any subsequent processing or analysis of the blood sample. Thus, the affinity molecules must be .situated in such a manner as to come into contact with the blood during the time of collection. As such, the affinity molecules may be situated to where they come into contact with the blood cither before or after the blood collects in the reservoir, so long as the affinity molecules contact the blood during the time of collection. The purpose of contacting the affinity molecules with the blood during the time of collection is to reduce the number of handling ana processing steps. [0055] Therefore, present invenlion provides a means of separating abundant proteins or other components from blood by adding, for example, polystyrene microparticles coated with antibodies spi.cifk to blood proteins or other components. I'he microparticles, for example, can be readily purchased from the manufacturer of microspheres and are designed for immunological applications. Such microspheres are sold with antibodies, without antibodies, or arc derivative-ready for antibody conjugation. [0056] The methods of the present invention can serve as an integrated and self-contained une-sicp ckyn-up tool or, alternatively, an integrated and self-contained one-step enrichment tool, for downstream testing during the blood collection process. Accordingly, the collection and removal of at least one undesircd component from the blood sample can be performed during the same procedure. After clot formation in serum (or no clot formation in plasma), the sample can optionally be subjected to a short waiting period for the binding reaction to take place to the coated polystyrene beads, for example. The time required for the reaction will depend on the kinetics associated with the binding for the intended targets and may be affected by other factors such as, but not limited to, diffusion or mixing. [0057] After exposure of the blood to the affinity molecules, the sample can then be ccntrifuged, for example, according to the manufacturer's recommendations, allowing the particles with the bound undesired component to settle out at the same rate or faster than the clot or other cellular portion of the blood. The affinity molecule-coated particles, for example, should be of similar density as the clot or the cells so that all of the bound components will settle out of the serum or the plasma. Alternatively, the density and size can be chosen so that the bound components will float. Particles coated with affinity molecules can be chosen based on specific sizes and densities. Accordingly, the methods of the present invention can remove most or all of the targeted components of the blood, based on the time of incubation, before centrifugation. The removal need not be complete such that 100% of the undesired component is removed from the blood. Rather, all that is required is that the removal of at least one component of the blood be to such a degree that will allow more accurate analysis and/or efficient processing of the blood, compared to blood where the undesired component has not been removed. The resulting plasma or serum, for example, should be sufficiently free of undesired components, and most other components should not be affected because of the speciiicity of the affinity molecules toward the specific components. [0058] In another embodiment, for example, tube walls can be coated with affinity molecules. In this case, the tube walls would function as a solid support, and the affinity molecules would be conjugated, covalently or non-covalently, to the polymers or particles of the tube. The targeted components would attach to the tube wall and remain there during any pre-analytic steps. Accordingly, samples would be enriched during the collection process and, after centrifugation, the enriched samples could be used directly from the primary tube or transferred to a secondary container. [0059] In another embodiment, as depicted in FIG. 14, the reservoir can be formed by a syringe barrel and stopper assembly. The syringe 20 would comprise syringe body 22 having distal end 27 and proximal end 25, a stopper 31 axially movable within the syringe body, and a distal end 30 for mating with a needle assembly 37. The distal end for mating with a needle assembly could, in one embodiment, comprise a frusta-conical tapered male luer 28, thereby providing a conduit from the reservoir to and through the distal male luer taper. Typically, a hypodermic needle comprising a female luer hub with a distally extending lumen forming a conduit from the female luer to the distal lumen tip, would be employed onto the frustoconical tapered male luer on the distal end of the syringe body. [0060] In other embodiments, the distal end for mating with a needle assembly could comprise an elastomeric access seal so that a cannula integral with a needle assembly can puncture the elastomeric access seal thus establishing fluid communication with the reservoir. The needle assembly would comprise both an elastomeric seal puncture cannula and a venous access cannula such as a stainless steel needle. Specifically, the elastomeric seal puncture cannula would pierce through the elastomeric access seal to establish fluid communication between the reservoir and a patient's vein. In certain embodiments, the needle assembly would have hook elements to reversibly mate to lugs radially extending from the distal portion of the syringe barrel. Examples of such devices are found, for example, in U.S. Patent No. 4,449,539, the disclosure of which is iiereby incorporated by reference. [0061 ] Svringe body 22 houses an axially movable stopper 31, as known in the art. Preferably, a plunger rod 32 integral to stopper 31 extends proximally from stopper 31 and can be manipulated by a user to move stopper 31 along the axis of syringe body 22. Movement of the stopper causes compression or expansion of the reservoir. According to the present invention, affinity molecules (not shown) are positioned inside the reservoir. In certain embodiments, the affinity molecules are placed on the inner sidewalls of the syringe body, hi other embodiments, the affinity molecules are attached to one or more inserts disposed w i i h i n the reservoir of the syringe assembly. It is possible to provide an initial -.pace lxiv,x-en UK; stopper and the distal end of the syringe body to provide space for such inserts to be present prior to collection. In further embodiments, affinity molecules are placed on the one or more inserts and on the inner sidewalls of the syringe. Preferably, the inserts would include microparticles or beads. In some embodiments, the inserts would be small enough to be able to exit the reservoir without disassembling the assembly such as t . v i i i n g ilv,: lumen extending through the male luer taper or the lumen of the cannula punciunnj.: UK '.-Lui.urneric seal described above. In other embodiments, the inserts would be large enouyh to be retained within the reservoir, or a mesh or filter can be provided to prevent exit 01 smaller inserts such as microparticles. In further embodiments, a combination of inserts large enough to be retained within the reservoir and small enough to be able to exit the reservoir delimited by the syringe barrel and stopper may be present. In the embodiments described above, the inserts can be comprised of particles that do and do not respond to magnetic fields placed near the syringe barrel assembly. [0062] Illustrated in FIG. 15 is another embodiment of the present invention in which the affinity molecules 410 are placed in a conduit 426 that communicates between a collection vessel and a venous access element. The venous access element may include catheters, blood collection sets, dialysis needle sets, fluid administration sets, hemodialysis needle sets, aphaeresis needles, blood bags attached to tubing with port access elements or skin puncture needles, and the like. In some embodiments, the venous access point ci.n directly access a vein through a purcutancous puncture or indirectly by accessing a port that is in fluid communication with a vein. The conduit is preferably a flexible section of polymer tubing such as FVC- Affinity molecules can be disposed inside the conduit directly by adherence to the conduit's inner sidewall surface 428. Alternately, affinity molecules can be disposed inside the conduit by disposing inserts such as beads or microparticles inside the conduit. The size and shape of the inserts can be such that they are maintained inside the conduit and are restricted front traveling freely within the conduit because of size restrictions or other elements added to retain their location. [0063] FIG. 1 5 shows a blood collection set 420 with an intravenous puncture needle 422, a reservoir access needle 424, and a conduit 426, which contains affinity molecules 410, extending therebetween. Integrated to intravenous puncture needle 422 is a wing element 430 extending laterally on both sides of the intravenous puncture needle. The wing element also establishes a hub 432 that helps interface conduit 426 to the inner lumen of intravenous puncture ivjsdle 422. Conduit 426 can directly or indirectly interface with reservoir access needle -\"1~\. Indirectly, conduit 426 can be integral with a female luered connector that is further integrated to a male luer hub bonded to the reservoir access needle. Preferably, surrounding reservoir access need;e 424 would be a collection container holder 440 that facilitates the connection of a bloo 1 collection tube or blood culture bottle to blood collection itt 420. Additionally, a shield elei v.'nt may be disposed on or about the distal portion of the conduit to provide shielding of the listal tip of the intravenous access needle. In additional embodiments, the intravenous punc ure needle can be replaced by a valve such as a lueractivated valve. Examples of luer a ;t.ivated valves include the Alarais SmartSite® Needleless valve sold by Alaris Medical S /stems and the Clave® valve sold by ICU Medical. Alternatively, the intravenous punc lire needle can be replaced by a stop-cock port or possibly a split septum port such as the Interlink® system sold by Baxter International, Inc. Examples [0064] Example 1 ('oating Evacuated Polystyrene Tubes with High Affinity Antibodies [0065\| High affinity antibodies (~10"8 M - ~10"12 M) directed toward, for example, albumin ("albumin antibodies") can be purchased from various suppliers. Polystyrene tubes are sterilized, and the albumin antibodies are covalently linked to the tubes as outlined in Tijssen, P., Practice and Theory of Enzyme ImmunoAssays, in Burdon, RH and Knippenberg, PH, (Eds.), Laboratory Techniques in Biochemistry and Molecular Biology, Elsevier Science Publishers. Vol. 15: 297-329 (1985), the entirety of which is hereby incorporated by reference- Briefly, (he tubes are pretreated with 0.2% (v/v) glutaraldchydc in 100 mM sodium phosphate buffer, pH 5.0, for 4 hours at room temperature. The tubes are then washed twice with the same buffer and an antibody solution, which is prepared by adding 2- 10 ug/mL in 100 mM sodium phosphate buffer, pH 8.0, is poured into the tubes and allowed to incubate for 3 hours at 37°C. After incubation, the tubes are washed twice with 0.9% NaCl. Alter washing, 100 mM lysine in sodium phosphate buffer, pH 8.0, is placed in the tubes for i hour at 37°C to block any remaining free aldehydes on the sides of the tubes. The tubes are then washed with several changes of PBS containing 0.05% Tween™ 20. The assembly and evacuation of the tubes is then completed as is standard in the art. [0066] Example 2 Collection of Blood In Coated Tubes [0067] Blood is drawn from a patient and collected in the coated evacuated tubes as prepared in Example 1. flic blood is allowed to incubate in the tube at room temperature for approximately 5-90 minutes (depending on the affinity and concentration the antibody used in coating the tubes), while the tubes are gently rocked or otherwise mixed to allow binding of albumin to the antibodies on the sides of the tubes. The tubes can be incubated overnight at room temperature. [0068]Example 3 •-• Testing the Ability of /lie Coated Tubes to Remove Albumin or Other Intci'fe rit ig /'/ ~o te u ?.v [0069] The blood collected in Example 2 is then centrifuged at 1100 G for about 10 minutes 10 separate the components. After component separation, the proteins in the albumindepleted plasma is subjected to 2D gel, as described in Celis, JE, and Bravo, R, (Eds.), Two- Dimemional gel Electraphoresix of Proteins, Academic Press (1984), the entirety of which is hereby incorporated by reference. 2D gel electrophoresis will separate the remaining proteins in the sample, based on molecular weight and isoelectric point. The separated proteins are then isolated and purified using the Montage In-Gel Digestgr, Kit™ (Millipore Corp.) according to the manufacturer's protocol. Proteins of interest are identified using Matrixassisted laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF MS) on an Axima CFR MALDI-TOF Mass Spectrometer (Kratos Analytical, Inc.), as described in Worrall, TA, et al., Anal, Chern. 70: 750 (1998), or using liquid chromatography. [0070] The amount of recovery of the protein of interest can be adjusted by varying the concentration and/or affinity of the antibody used in Example 1. [0071] Example 4 - Covalent Linkage of Antibodies to Polystyrene Beads [0072] Add 5mL of 12.5% (v/v) glutaraldehyde in 50 mM sodium phosphate, pH 7.0, to about 25 hydrazide derivatized polystyrene beads (6.4mm diameter; Pierce Chemical Company) and shake very gently for about 2 hours at room temperature. The beads are then washed in a Buchner funnel (without filter paper) with lOOmL water and with 20 mL of 100 mM sodium phosphate buffer, pli 6.0. Next, the glutaraldehyde-activated beads arc placed in an antibody solution prepared by dissolving 2.5mg Ab in 5mL of sodium phosphate buffer, pH 6.0. After the beads are in the Ab solution, add 1 mg NaCNBH3 to the solution. Shake gently overnight at room temperature. After shaking, the beads are washed with lOOmL of 100 mM sodium phosphate buffer, pH 6.0. and then washed with 50 mL of 100 rnM NaHCO) The residual aldehydes on the beads are blocked by incubating the beads with 5mL of 100 mM lysirie lor about 1 hour at room temperature. After incubating with lysine, the beads are washed with lOOrnL of lOOmM Na2CO3 and with lOOmL water and dried. The Abcoated beads can be stored dry at 4°C. The method above is disclosed in Tijssen, P., Practice and Theory of Enzyme ImmunoAssays, in Burdon, RH and Knippenberg, PH, (Eds.), Laboratory Techniques in Biocheinwtiy and Molecular Biology, Elsevier Science Publishers, Vol. 15(1935). [0073] While various embodiments of the present invention have been described herein, it should be understood that these examples and embodiments have been presented by way of example only, and not limitation. Thus, the breadth and scope of the present invention should not be limited by any of the above-described exemplary embodiments. We Claim: 1. A device for collecting blood comprising a reservoir and affinity molecules, wherein said affinity molecules are exposed to said blood during the time of collecting said blood, wherein said affinity molecules are completely contained within said reservoir and, wherein said affinity molecules are coated onto the interior of said reservoir. 2. The device as claimed in claim 1, wherein said affinity molecules are coated onto the interior of side walls of said reservoir. 3. The device as claimed in claim 2, wherein said interior side walls are ridged. 4. The device as claimed in claim 1, wherein said affinity molecules have an affinity towards undesired targets. 5. The device as claimed in claim 1, wherein said affinity molecules have an affinity towards desired targets. 6. The device as claimed in claim 1, wherein said affinity molecules are selected from the group consisting of antibodies, antibody fragments, enzymes, fragments of enzymes, enzyme substrates, fragments of enzyme substrates, nucleotides, oligonucleotides, polynucleotides, receptors, aptamers, nanobodies, fragments of receptors, ligands, fragments of enzymes, other proteins, amino acids, peptides, polypeptides, oligopeptides, saccharides, disaccharides, polysaccharides, glycoproteins, proteoglycans, and natural and synthetic polymers. 7. The device as claimed in claim 6, wherein said affinity molecules are antibodies or fragments thereof. 8. The device as claimed in claim 7, wherein said antibody or fragment thereof binds at least one protein within said blood. 9. The device as claimed in claim 8, wherein said at least one protein is selected from the group consisting of albumin, immunoglobulins, fibrinogen, protease inhibitors, antichymotrypsin, antithrombin, macroglobulins, inter-alpha-trypsin inhibitor, CI inhibitor, plasmin inhibitor, heparin cofactor II, tranferrin, immunoglobulin M, haptoglobin complement and alpha-1 antitrypsin. 10. The device as claimed in claim 9, wherein said protein is albumin.

Full Text

DEVICE FOR COLLECTING BLOOD, FOR COMPONENT REMOVAL DURING BLOOD COLLECTION
Background of the Invention
Field of Invention
[0001] The present invention relates to devices for collecting blood, for component removal during blood collection, whereby certain target components are isolated or removed from the blood sample at the time of collecting the blood, as well as methods of using such devices.
Background of the Invention
[0002] Today with the growth of proteomics, large-scale isolation and identification of proteins is becoming the focus of research, drug development and diagnostics. Protein separation methods are well-established in the molecular and biochemical arts; however, with the growth of proteomics, new technologies have arisen which require different, more stringent protein separation techniques. For example, exploring the plasma proteome requires the separation and identification of low abundance proteins and the determination of their characteristics using electrophoresis in a 2-dimensional (2D) gel system. However, plasma proteins or other components present in high concentrations, for example albumin, can hinder the separation and obscure the identification of these lower abundance proteins and, at times, make it difficult or impossible to determine their identity on 2D gels or by any other downstream testing of blood samples.
[0003] Currently, many different methods exist to remove albumin and other high abundance components from blood samples; however, most of these clean-up methods are not specific enough and may actually remove many of the desirable proteins or components. Current albumin clean-up methods, for example, require transferring the sample into another container, thus requiring additional handling steps, and increasing the risk of processing errors, sample contamination, and extra risk of operator exposure to possibly infectious blood components. Furthermore, current methods do not use any kind of specifically directed affinity molecule targeting the target components in the clean-up process, and may thus remove components of interest. Accordingly, there is a need for blood sample collection

devices, methods of using such devices, and processes that eliminate the risk of error and
contamination, while also providing specific isolation of target components.
Summary of the Invention
100041 The present invention relates to a device for collecting blood comprising a reservoir
and a f f i n i t y molecules, wherein the affinity molecules arc exposed to blood during the time
of blood collection,
[0005] The present invention also relates to a method of collecting blood comprising
exposing the blood to affinity molecules during the time of blood collection,
Brief Description of the Drawings
(0006) FIG. 1 depicts one of the devices of the present invention.
J0007] FIG. ." is a longitudinal sectional view of the device of FIG. 1.
|OOU8| FIG. •• is a longitudinal sectional view of the device of FIG. 1, illustrating fluid
Jcin try into the device.
[0009] FIG. 'I illustrates the device of FIG. 1 during centrifugation, with the subsequent
release of the separator.
[0010| FIG. 5 also illustrates the device of FIG. 1 during centrifligation, with the subsequent
re least of the separator.
|00l 1] FIG. t) illustrates the device of FIG. 1 after centrirugation, showing the separation of
(he l i q u i d sample Into higher and lower specific gravities.
[0012] FIG. 7 depicts another device of the present invention.
[0013] FIG. 8 is a longitudinal sectional view of the device of FIG. 7.
[0014] FIG. 9 is a longitudinal sectional view of the device of FIG. 7, illustrating fluid
delivery into the device.
[0015] FIG. 10 illustrates the device of FIG. 7 during centrifugation, with subsequent release
of the separator.
[|0()16| FIG. 1 1 also illustrates the device of FIG. 7 during centrifugation, with subsequent
release of the separator,
[0017] FIG. 12 illustrates the device of FIG. 7 after centrifugation, showing separation of the
liquid sample into higher and lower specific gravities.
[0018| FIG. 13 depicts another device of the present invention.
[0019] FIG. 14 depicts another device of the present invention, wherein antibody-coated
beads are placed within a syringe assembly.
|0020] FIG. ! 5 depicts another device of the present invention, wherein antibody-coated
tubing is used for in-line component removal.
Detailed Description of the Invention
[0021) 'Hie present invention relates to a device for collecting blood comprising a reservoir
and a f f i n i ty molecules, wherein the affinity molecules are exposed to the blood during the
nine of collecting the blood.
[0022) The present invention also relates to a method of collecting blood comprising
exposing the blood to affinity molecules during the time of said collection. The affinity
molecules will bind target molecules present in the blood. As used herein, "target molecules"
or "targets" is used to mean a component that may or may not be present in the actual sample
of blood, but will bind to the affinity molecules if present.. The target may be "desired" in
that Ihe moltcule targeted by the o Trinity molecule is of interest for further processing or
assaying. Alternatively, the target may be "undesired" in that the target may be considered
an impurity, or may be unnecessar' or unwanted in further processing or assaying steps.
J0023| As used herein, "blood" is intended to mean whole blood, or the separate components
of whole blood, from any animal. Separate components of blood include, but are not limited
to, plasma and serum, the definitions of which are used herein as these terms are used in the
art. For example, plasma is the component of whole blood that is substantially free of cells,
including plaielels. Serum is plasma in which fibrinogen and other clotting factors have been
removed. Thus, "blood," as used herein, can include whole blood, plasma, serum, the
cellular portion of blood and even the protein portion of whole blood. Furthermore, for the
purposes of this invention, components of blood can be isolated, such as the isolation of
protein or other molecules from blood such as an isolate of fibrinogen, or can be a mixture of
molecules, such as plasma or serum. For example, as used in the current context, albumin is
considered a component of blood. As used herein, "component" does not include the cellular
portion of blood, whereas, "cellular component" includes cellular portions. Cellular
components may or may not include, and are not limited to, hematopoetic cells, myeioid stem
cells, erythrocytes, neutrophils, basophils. eosinophils, megakaryocytes, platelets, monocytcs,
mast ceils, lymphoid cells, including all T-cells and B-cells, normal or diseased cells from
any body tissue.
[0024| Furthermore, "collecting" is used to mean extraction of the blood from its normal
environment, whether or not the blood is returned to the individual from which it was
extracted, or placed into a different individual, such as during a transfusion. As used herein,
the terms "individual," "subject" and "patient" are used interchangeably and are used to mean
any animal, preferably a mammal, more preferably a human or non-human primate.
[0025] The device of the present invention comprises a reservoir. The reservoir can be any
device, open ur closed to the environment, that can hold any amount of blood, at least
temporarily. Of c reservoir can also be capable of being punctured and resealed. Examples of reservoirs of the
current invention include, but are not limited to, evacuated tubes, non-evacuated tubes, petri
dishes, microtiter plates, syringe barrels, needles, machines that can process or analyze blood,
blood collect ion systems such as blood collection bags, such as the type used when donating
blood, iiiid even plastic tubing.
[0026] The blood need not be stationary inside the reservoir, but can be sedentary, or moving
or flowing, la one embodiment, the reservoir comprises a density gradient barrier wherein
the sample collected within the reservoir is separated into higher and lower densities during
centrifugation. Any type ofblood collection device or system may be used as a reservoir.
Suitable devices and systems that can act as reservoirs include, but are not limited to, blood
tubes manufactured by, for example, Becton Dickinson and Company (BD) including, but not
limited to, Serum Clot Activator Tubes, Serum Gel Clot Activator Tubes, Lithium Heparin
Tubes, Sodium Fluoride Tubes, Sodium Citrate Tubes, ESR Citrate Tubes and Trace Mettal
Tubes. Additionally, capillary blood collection systems produced by any manufacturer
including, but not limited to, Microvette™ systems can act as reservoirs. Examples of some
types of reservoirs contemplated for use in the present invention are contained in U.S. Patent
No. 6,479,298, the entirety of which is incorporated herein by reference.
[0027] In one embodiment, the reservoir further comprises an insert. As used herein, the
term "insert" means a material that is completely encapsulated by the reservoir, such as a
solid dipstick, mechanical density partition element, or a paddle within an evacuated tube.
The term "insert" can also refer to material that is only partially within the reservoir. The
inserts can be a solid object, such as a paddle, or the inserts can be a liquid, a gel or a gas.
Other examples ot inserts include, but are not limited to, beads, particles and microparticlcs.
Examples of beads include, but are not limited to, paramagnetic beads that can be
manipulated by a magnetic field. The inserts can be any shape, provided that at least a portion
of the insert is located within the reservoir. Examples of the shapes of inserts include, but are
not limited to, rectangular, circular, oval, straight rod and helical-shaped rod.
10028] In another embodiment, the interior walls of the reservoir can be smooth or ridged.
Providing a reservoir with ridges serves to increase the interior surface area of the reservoir.
In yet another embodiment, the inserts are also ridged to increase their respective surface
areas. In still another embodiment, both the insert and the interior walls of the reservoir arc
ridged to provide even more surface area. The purpose of increasing the surface area in any
of the embodiments of the present invention is to increase the amount of surface-immobilized
affinity molecules that can come into contact with the blood at the time of its collection.
[0029] In addition to the reservoir, the device of the present invention also comprises one or
more affinity molecules. As used herein, "affinity molecules" can be any type of molecule
that, possesses an affinity towards single or multiple components of the blood. The affinity
can be a specific affinity in that the affinity molecule has an affinity for a focused subset of
components, such as an antibody specific for fibrinogen. The affinity of the affinity
molecules can also be less specific in nature, such as a protein that binds the general class
immunoglobulins, for example, protein A. Even further, the affinity molecules as
contemplated by the present invention can exhibit an even broader affinity such that the
affinity molecules can possess an affinity for a mixture of different classes of proteins.
[0030] Examples of affinity molecules include, but are not limited to, antibodies, antibody
fragments, enzymes, fragments of enzymes, enzyme substrates, fragments of enzyme
substrates, nucleotides, oligonucleotides, polynucleotides, receptors, fragments of receptors,
ligands. fragments of enzymes, other proteins, amino acids, peptides, polypeptides,
oligopeptides, saccharides, disaccharides, polysaccharides, glycoproteins, proteoglycans,
natural and synthetic polymers, aptamers, and nanobodics. [n one embodiment, the affinity
molecules arc antibodies, or fragments of antibodies, such as, but not limited to, Fab
fragments, FiulV'b fragments, and scFv fragments. The antibodies may be polyclonal,
monoclonal, chimeric, synthetic or naturally occurring.
[0031] In another embodiment, the affinity molecules of the present invention bind a
component within the blood. Preferably, the affinity molecules bind a blood protein.
Examples of proteins that the affinity molecules will bind include, but are not limited to,
fibrinogen, albumin, immunoglobulins including, but not limited to, alpha-, beta- and
gamma-globulins, proteases such as chymotrypsin and thrombin, and protease inhibitors such
as antichymotrypsin, untithrombin, macroglobulins, intcr-alpha-trypsin inhibitor, Cl
i n h i b i t o r , plusmin inhibitor, hcparin cofactor II, apoprotein A and fibronectin.
[0032) In OIK: embodiment, the affinity molecule or set of affinity molecules can bind to
more tlv.in one nudes i red component at the same lime, resulting in more than one undcsired
component ixmg removed from the sample. In another embodiment, the affinity molecule or
set ol .'affinity molecules can bind lo more than one desired component at the same time,
resulting in more than one desired component being isolated. In another embodiment, more
than one affinity molecule or set of affinity molecules can be employed, with each affinity
molecule or set of molecules binding different undesired components, resulting in more than
one uiHiesiruJ component being removed from the lampk. Of course, the invention
encompasses ut.iliv.ing one affinity molecule or set of affinity molecules that can bind only
one undcsired component. Optionally, the invention encompasses utilizing one affinity
molecule or a set of affinity molecules that can enrich the sample by removing all but a few
desired components from the original specimen. As used herein, the phrase "a set of affinity
molecules" is used to mean a plurality of affinity molecules that possess the identical type of
affinity, in both specificity and strength. As used herein, "affinity molecules" is used to mean
one or more affinity molecules and one or more sets of affinity molecules. In one
embodiment, the invention encompasses a reservoir and a single device, comprising an
affinity molecule or a set of affinity molecules. In another embodiment, the invention also
encompasses a single device, comprising a mixture of more than one distinct affinity
molecules or sets of molecules. In yet another example, the invention also comprises
multiple devices, with each device comprising a distinct affinity molecule or set of affinity
molecules directed towards, for example, itnmunoglobulins and clotting cactors. In still
another embodiment, the invention also comprises multiple devices, witu each device
comprising a mixture of more than one distinct affinity molecules or sets of affinity
molecules. In still another embodiment, the invention also comprises a kit of multiple
devices, with each device comprising a mixture oi more than one distinct affinity molecules
or sets of affinity molecules.
[0033] The affinity molecules of the present invention can be present in soluble or
immobilized rbrm. If. for example, the affinity molecules arc in soluble form, they may be
present in the reservoir in liquid or lyophilized form. The blood would then be added to the
solubili/.ed ailinitv molecules, which could then be pulled oat of the sample or fractionated
from the sample depending on the specific user needs for the final sample. For example, the
aifinity molecules can be bound or attached to inert substances such as, for example, glass,
polypropylene, nylon, nitrocellulose, polymcthacrylate, polystyrene, polyvinylchloride,
styrenebutadiene copolymer, styrene-acrylatc copolymer, latex, chemically modified plastic,
rubber, red blood cells, -_i polymeric material or biological cells. According to the present
invention, examples of the surfaces on which the inert substances can be found include, but
are not limited to, any portion of the reservoir, the sides and/or bottoms of tubes, the sides
and/or bottom of culture dishes, aay type of insert such as a paddle, dipstick, gel, beads,
particles, microparticles, and the I ning of tubing. The affinity molecules can be attached to
the surfaces by any known method including, but not limited to. covalent and non-covalenl
oTtachnitiit or coupling, such as at! iching the affinity molecules directly to the inert surfaces
or attaching the affinity molecules o derivatized and/or coated surfaces.
|0034| In one embodiment, microp irticles such as beads are used in the present invention,
with the beads comprising scpharo.ie or polystyrene. In another embodiment, the beads may
comprise magnetic particles, such as magnetic, paramagnetic, and superparamagnetic beads
produced by Dynal AS (Oslo, Norway) and sold as DYNABEADS™. If magnetic beads are
used, regard le >s o! the manufacturer, a magnet could be used to manipulate the movement of
the proteins bound to the beads. The magnet can further be used to collect or separate the
beads from the rest of the blood sample.
[0035] In another embodiment of the present of the invention, a density separator gradient
may be introduced to further separate density phases of a collected sample. Such density
gradients may be chosen from the group consisting of a thixotropic gel and a mechanical
separator. Additionally, beads or microparticles may be chosen such that their density is
greater than the density separator. For instance, if the density of the separator is about 1.02 to
about 1.08 g/cin', the density of the beads may be chosen such that all or a portion of the
beads have a density greater than about 1.08 g/cnr\ The higher density beads should come to
rest at the bottom of the container during ccntrifugation, thus moving a portion of the bound
targets below the density separator. When the targeted molecules are highly abundant,
removal of the beads or microparticles will thus enrich the sample remaining in the reservoir.
In another embodiment of the present invention, beads may be chosen such that their density
is smaller than the density of a density separator. For instance, if the density of the separator
is about i .02 to about 1.08 g/cnr , the density of the beads may be chosen such that all or a
portion ot the beads have a density lower than about 1.02 g/cm3. The lower density beads
should iloat to the top of the sample, thus moving a portion of the bound targeted proteins
above the separator. When the targeted molecules are highly abundant, removal of the beads
or microparticles will thus enrich the sample remaining in the reservoir.
[0036] The ih.ixotropic gel separator may be applied to the inner surface the reservoir.
Altermnely, ;i mechanical separator may be inserted within the tube to separate fluids. Once
the blood collection tube is subjected to centrifugal force, the separator will then move up or
down the tube as it comes into a density equilibrium with the blood sample, thus acting to set
up a density gradient for the blood. In conjunction with the separator, the beads, if used in
Hie present invention, can also act within and help establish a density gradient to allow ease
uf separation of tht- various components of the blood.
[0037] The present invention may be embodied in other specific forms and is not limited to
any specific embodiments described in detail, which are merely exemplary. Various other
modifications will be apparent to and readily made by those skilled in the art without
departing from the scope and spirit of the invention. The scope of the invention will be
measured by the appended claims and their equivalents.
[0038] One embodiment of the present invention is illustrated in FIGS. 1-6, wherein
assembly 120 comprises a reservoir 130 (here, depicted as a tube), a closure 150 and a
separator 170.
[0039] Reservoir 130 has an open end 132 that includes a top edge (not shown), a closed end
134 and a sidevvall 136 extending between the open end and the closed end. Sidewall 136 has
an outer surface 138 and an inner surface 140. Reservoir 130 defines a receptacle with a
central axis "A". Assembly 120 includes affinity molecules 131 located on inner surface 140
of sidewall 136.
|0040| Closure 150 is disposed to fit over open end 132 of reservoir 130. Closure 150
comprises an annular upper portion 152, which extends over the top edge of sidewall 136,
and a lower annular portion or skirt 154 of lesser diameter than the annular upper portion
152, which extends into and forms an interference fit with inner surface 140 of sidewall 136
for maintaining closure 150 in place in open end 132.
[0041] Annular upper portion 152 includes a top surface area 156, sidewall 158 that
converges from surface area 156 towards upper well area 160. Lower annular skirt portion
154 defines a lower well 162, an inner wall surface 164, an outer wall surface 166 and a
bottom surface 168, Well area 160 and lower well area 162 define a thin diaphragm or selfsealing
septum through which a needle may be inserted. The self-sealing septum material
allows penetration by a piercing element such as a needle and then rcseals when the piercing
element is withdrawn.
[0042] An annular ledge or abutment 157 separates annular upper portion 152 and lower
annular portion 154,
[0043] Preferably, closure 150 is made of natural rubber elastomer, synthetic thermoplastic
and ihermoset elastomeric materials. Preferably, the closure is made of a resilient elastomeric
material whereby the septum is self-sealing.
[0044] As shown in FIG. 3, a liquid sample A is delivered to the tube by a needle that
penetrates closure 150 in upper well area 160. For purposes of illustration only, the liquid
sample is blood. The liquid sample is delivered into the passageway of separator 170 so that
the liquid sample is introduced between closed end 134 of reservoir 130 and separator 170,
whereby the outer surface of all components of separator 170 are substantially free of any
contact with the fluid sample.
[0045] As shown in FIGS. 4 and 5, when assembly 120 is subjected to centriftigation or axial
centrifugation force, seal body 191 of separator 170 deflects, thereby reducing its diameter
and eliminating its interference lit with the inner wall of the reservoir. Separator 170 releases
from the inner wail of the reservoir such that separator 170 descends towards closed end 134
of reservoir 1 30. This also opens up a path 300 between the reservoir and the separator,
permitting the tlow of the low-density component B of the fluid upwardly past the separator
as the separator migrates down the reservoir. The low-density component inside the
passageway i 99 of the separator will migrate downwardly and upwardly past the separator.
Thus, separator 170 is permitted to sink into the fluid sample.
(0040] As shown in FIG. 6, after ccntrifugation is terminated, the absence of the centrifugal
load will cause seal body 191 to resiliency return toward an underformed condition and
lightly seal with the inner wall of the tube. Thus, separator 170 serves as a divider between
lower specific gravity portion B and higher specific gravity portion C of the liquid sample.
|0047| FIGS. 7-12 represent an alternative embodiment of the present invention. The
embodiment of FIGS. 7-12 operates in the same manner as the embodiment of FIGS. 1-6, but
contains beads 135' w i th a f f i n i ty molecules 131' thereon, as well as affinity molecules 131'
1'ieutxd on inner surface 140' of sidcwall 136'. Elements common lo the embodiment of
I ' I G S . l-o and FIGS. 7-12 are numbered identically, with those of the latter embodiment
denoted as "prime': (').
[0048] FIG. 13 shows the interaction of the device of FIGS. 1-6 with a holder 42. Holder 42
has a cannula 44 with a venous entry end 48 and a non-patient end 46 such that when
:i>sembly 120 i? inserted into holder 42, the self-scaling septum on closure 150 is pierced by
non-puii'_ nt tud 4o, thereby starting the process of collecting blood into reservoir 130.
10049] In one embodiment, the reservoir is compatible with the numerous additives used in
the art such us citrates, silicone. silicates, ethylenediaminctetraacctic acid (EDTA) and the
like. The additives are used, for example, to condition the sample either to facilitate or retard
clotting or to preserve the sample for a particular analysis. It is within the purview of the
present invention that one or more additives may be used.
[0050] The affinity molecules themselves may or may not be modified to allow ease of
separation. For example, the affinity molecules may be biotinylated, and streptavidin could
be used to remove the biotinylated affinity molecules after having bound the target
component. Thus, a component can be removed from a sample first by exposing the affinity
molecules to the sample at the time of collection and subsequently using biotinylated
anti-affinity molecule antibodies. The biotinylated antibodies would then be captured using,
for example, avidin, streptavidin, or Neutravidin™, resulting in a sampL substantially
depleted of the targeted component.
[0051] In one embodiment, the affinity molecules are found within the reservoir. For
example, the affinity molecules may be bound to beads, which are found within the reservoir.
Thus, tlic invention can comprise an evacuated blood collection device with, for example,
antibody-coated beads added to the tube. The tube containing the beads would be used, for
example, during venipuncture blood collection procedures in human.s and/or animals. In a
similar embodiment, the invention could also be, tor example, non-evacuated microcollection
containers containing antibody-coated beads. The foregoing embodiment can be
modified, for example, by substituting the beads for any inert substance or surface, whether
or not the inert substance or surface is listed herein.
[0052] In another embodiment, the affinity molecules may be external to the reservoir. For
example, the affinity molecules may be bound to beads that are external to the reservoir and
m;i'-' comprise, for example, an affinity column packed with affinity beads. Thus, the
invention can also comprise a reservoir for collecting the blood and one or more affinity
columns linked, in sequence, before or after and optionally integrated to the reservoir. The
foregoing embodiment can be modified, lor example, by substituting the beads for any
portion of the reservoir, the sides tnd/or bottoms of tubes, particles, microparticles, the sides
and/or bottom of culture dishes and the lining of tubing.
[0053] Indeed, lilt, loregoing embo liments can be modified, for example, by substituting the
beads for any men substance or sur ace, whether or not the inert substance or surface is listed
herein. Further, all of the cmbodim ;nts described herein can also be modified by substituting
antibodies, for example, with any t} pe of affinity molecule such as antibody fragments,
enzymes, fragments of enzymes, crzyme substrates, fragments of enzyme substrates,
nucleotides, oligonucleotides, polynucleotides, receptors, fragments of receptors, ligands,
fragments of enzymes, other proteins, amino acids, pcptides, polypeptides, oligopeptides,
saccharides, di.saccharides, polysaccharides, glycoproteins, proteoglycans, and natural and
synthetic polymer*-.. Further, all of the embodiments described herein can be modified by
substituting protein for any undesirable component of blood.
[0054] The present invention provides distinct advantages over current methods, these
advantages being achieved by exposing the affinity molecules to the blood during and/or
immediately alter the blood collection process. As used herein, "during the time of
collection" is used to mean that the affinity molecules are exposed to the blood during the
blood collection process, The exposure of the blood to the affinity molecules need not be
briefer instantaneous in time, so long as the exposure of the biood to the affinity molecules
occurs prior to any subsequent processing or analysis of the blood sample. Thus, the affinity
molecules must be .situated in such a manner as to come into contact with the blood during
the time of collection. As such, the affinity molecules may be situated to where they come
into contact with the blood cither before or after the blood collects in the reservoir, so long as
the affinity molecules contact the blood during the time of collection. The purpose of
contacting the affinity molecules with the blood during the time of collection is to reduce the
number of handling ana processing steps.
[0055] Therefore, present invenlion provides a means of separating abundant proteins or
other components from blood by adding, for example, polystyrene microparticles coated with
antibodies spi.cifk to blood proteins or other components. I'he microparticles, for example,
can be readily purchased from the manufacturer of microspheres and are designed for
immunological applications. Such microspheres are sold with antibodies, without antibodies,
or arc derivative-ready for antibody conjugation.
[0056] The methods of the present invention can serve as an integrated and self-contained
une-sicp ckyn-up tool or, alternatively, an integrated and self-contained one-step enrichment
tool, for downstream testing during the blood collection process. Accordingly, the collection
and removal of at least one undesircd component from the blood sample can be performed
during the same procedure. After clot formation in serum (or no clot formation in plasma),
the sample can optionally be subjected to a short waiting period for the binding reaction to
take place to the coated polystyrene beads, for example. The time required for the reaction
will depend on the kinetics associated with the binding for the intended targets and may be
affected by other factors such as, but not limited to, diffusion or mixing.
[0057] After exposure of the blood to the affinity molecules, the sample can then be
ccntrifuged, for example, according to the manufacturer's recommendations, allowing the
particles with the bound undesired component to settle out at the same rate or faster than the
clot or other cellular portion of the blood. The affinity molecule-coated particles, for
example, should be of similar density as the clot or the cells so that all of the bound
components will settle out of the serum or the plasma. Alternatively, the density and size can
be chosen so that the bound components will float. Particles coated with affinity molecules
can be chosen based on specific sizes and densities. Accordingly, the methods of the present
invention can remove most or all of the targeted components of the blood, based on the time
of incubation, before centrifugation. The removal need not be complete such that 100% of
the undesired component is removed from the blood. Rather, all that is required is that the
removal of at least one component of the blood be to such a degree that will allow more
accurate analysis and/or efficient processing of the blood, compared to blood where the
undesired component has not been removed. The resulting plasma or serum, for example,
should be sufficiently free of undesired components, and most other components should not
be affected because of the speciiicity of the affinity molecules toward the specific
components.
[0058] In another embodiment, for example, tube walls can be coated with affinity
molecules. In this case, the tube walls would function as a solid support, and the affinity
molecules would be conjugated, covalently or non-covalently, to the polymers or particles of
the tube. The targeted components would attach to the tube wall and remain there during any
pre-analytic steps. Accordingly, samples would be enriched during the collection process
and, after centrifugation, the enriched samples could be used directly from the primary tube
or transferred to a secondary container.
[0059] In another embodiment, as depicted in FIG. 14, the reservoir can be formed by a
syringe barrel and stopper assembly. The syringe 20 would comprise syringe body 22 having
distal end 27 and proximal end 25, a stopper 31 axially movable within the syringe body, and
a distal end 30 for mating with a needle assembly 37. The distal end for mating with a needle
assembly could, in one embodiment, comprise a frusta-conical tapered male luer 28, thereby
providing a conduit from the reservoir to and through the distal male luer taper. Typically, a
hypodermic needle comprising a female luer hub with a distally extending lumen forming a
conduit from the female luer to the distal lumen tip, would be employed onto the frustoconical
tapered male luer on the distal end of the syringe body.
[0060] In other embodiments, the distal end for mating with a needle assembly could
comprise an elastomeric access seal so that a cannula integral with a needle assembly can
puncture the elastomeric access seal thus establishing fluid communication with the reservoir.
The needle assembly would comprise both an elastomeric seal puncture cannula and a venous
access cannula such as a stainless steel needle. Specifically, the elastomeric seal puncture
cannula would pierce through the elastomeric access seal to establish fluid communication
between the reservoir and a patient's vein. In certain embodiments, the needle assembly
would have hook elements to reversibly mate to lugs radially extending from the distal
portion of the syringe barrel. Examples of such devices are found, for example, in U.S.
Patent No. 4,449,539, the disclosure of which is iiereby incorporated by reference.
[0061 ] Svringe body 22 houses an axially movable stopper 31, as known in the art.
Preferably, a plunger rod 32 integral to stopper 31 extends proximally from stopper 31 and
can be manipulated by a user to move stopper 31 along the axis of syringe body 22.
Movement of the stopper causes compression or expansion of the reservoir. According to
the present invention, affinity molecules (not shown) are positioned inside the reservoir. In
certain embodiments, the affinity molecules are placed on the inner sidewalls of the syringe
body, hi other embodiments, the affinity molecules are attached to one or more inserts
disposed w i i h i n the reservoir of the syringe assembly. It is possible to provide an initial
-.pace lxiv,x-en UK; stopper and the distal end of the syringe body to provide space for such
inserts to be present prior to collection. In further embodiments, affinity molecules are
placed on the one or more inserts and on the inner sidewalls of the syringe. Preferably, the
inserts would include microparticles or beads. In some embodiments, the inserts would be
small enough to be able to exit the reservoir without disassembling the assembly such as
t . v i i i n g ilv,: lumen extending through the male luer taper or the lumen of the cannula
punciunnj.: UK '.-Lui.urneric seal described above. In other embodiments, the inserts would
be large enouyh to be retained within the reservoir, or a mesh or filter can be provided to
prevent exit 01 smaller inserts such as microparticles. In further embodiments, a combination
of inserts large enough to be retained within the reservoir and small enough to be able to exit
the reservoir delimited by the syringe barrel and stopper may be present. In the embodiments
described above, the inserts can be comprised of particles that do and do not respond to
magnetic fields placed near the syringe barrel assembly.
[0062] Illustrated in FIG. 15 is another embodiment of the present invention in which the
affinity molecules 410 are placed in a conduit 426 that communicates between a collection
vessel and a venous access element. The venous access element may include catheters,
blood collection sets, dialysis needle sets, fluid administration sets, hemodialysis needle sets,
aphaeresis needles, blood bags attached to tubing with port access elements or skin puncture
needles, and the like. In some embodiments, the venous access point ci.n directly access a
vein through a purcutancous puncture or indirectly by accessing a port that is in fluid
communication with a vein. The conduit is preferably a flexible section of polymer tubing
such as FVC- Affinity molecules can be disposed inside the conduit directly by adherence to
the conduit's inner sidewall surface 428. Alternately, affinity molecules can be disposed
inside the conduit by disposing inserts such as beads or microparticles inside the conduit.
The size and shape of the inserts can be such that they are maintained inside the conduit and
are restricted front traveling freely within the conduit because of size restrictions or other
elements added to retain their location.
[0063] FIG. 1 5 shows a blood collection set 420 with an intravenous puncture needle 422, a
reservoir access needle 424, and a conduit 426, which contains affinity molecules 410,
extending therebetween. Integrated to intravenous puncture needle 422 is a wing element
430 extending laterally on both sides of the intravenous puncture needle. The wing element
also establishes a hub 432 that helps interface conduit 426 to the inner lumen of intravenous
puncture ivjsdle 422. Conduit 426 can directly or indirectly interface with reservoir access
needle -\"1~\. Indirectly, conduit 426 can be integral with a female luered connector that is
further integrated to a male luer hub bonded to the reservoir access needle. Preferably,
surrounding reservoir access need;e 424 would be a collection container holder 440 that
facilitates the connection of a bloo 1 collection tube or blood culture bottle to blood collection
itt 420. Additionally, a shield elei v.'nt may be disposed on or about the distal portion of the
conduit to provide shielding of the listal tip of the intravenous access needle. In additional
embodiments, the intravenous punc ure needle can be replaced by a valve such as a lueractivated
valve. Examples of luer a ;t.ivated valves include the Alarais SmartSite® Needleless
valve sold by Alaris Medical S /stems and the Clave® valve sold by ICU Medical.
Alternatively, the intravenous punc lire needle can be replaced by a stop-cock port or possibly
a split septum port such as the Interlink® system sold by Baxter International, Inc.
Examples
[0064] Example 1 ('oating Evacuated Polystyrene Tubes with High Affinity Antibodies
[0065| High affinity antibodies (~10"8 M - ~10"12 M) directed toward, for example, albumin
("albumin antibodies") can be purchased from various suppliers. Polystyrene tubes are
sterilized, and the albumin antibodies are covalently linked to the tubes as outlined in Tijssen,
P., Practice and Theory of Enzyme ImmunoAssays, in Burdon, RH and Knippenberg, PH,
(Eds.), Laboratory Techniques in Biochemistry and Molecular Biology, Elsevier Science
Publishers. Vol. 15: 297-329 (1985), the entirety of which is hereby incorporated by
reference- Briefly, (he tubes are pretreated with 0.2% (v/v) glutaraldchydc in 100 mM
sodium phosphate buffer, pH 5.0, for 4 hours at room temperature. The tubes are then
washed twice with the same buffer and an antibody solution, which is prepared by adding 2-
10 ug/mL in 100 mM sodium phosphate buffer, pH 8.0, is poured into the tubes and allowed
to incubate for 3 hours at 37°C. After incubation, the tubes are washed twice with 0.9%
NaCl. Alter washing, 100 mM lysine in sodium phosphate buffer, pH 8.0, is placed in the
tubes for i hour at 37°C to block any remaining free aldehydes on the sides of the tubes. The
tubes are then washed with several changes of PBS containing 0.05% Tween™ 20. The
assembly and evacuation of the tubes is then completed as is standard in the art.
[0066] Example 2 Collection of Blood In Coated Tubes
[0067] Blood is drawn from a patient and collected in the coated evacuated tubes as prepared
in Example 1. flic blood is allowed to incubate in the tube at room temperature for
approximately 5-90 minutes (depending on the affinity and concentration the antibody used
in coating the tubes), while the tubes are gently rocked or otherwise mixed to allow binding
of albumin to the antibodies on the sides of the tubes. The tubes can be incubated overnight
at room temperature.
[0068]Example 3 •-• Testing the Ability of /lie Coated Tubes to Remove Albumin or Other
Intci'fe rit ig /'/ ~o te u ?.v
[0069] The blood collected in Example 2 is then centrifuged at 1100 G for about 10 minutes
10 separate the components. After component separation, the proteins in the albumindepleted
plasma is subjected to 2D gel, as described in Celis, JE, and Bravo, R, (Eds.), Two-
Dimemional gel Electraphoresix of Proteins, Academic Press (1984), the entirety of which is
hereby incorporated by reference. 2D gel electrophoresis will separate the remaining proteins
in the sample, based on molecular weight and isoelectric point. The separated proteins are
then isolated and purified using the Montage In-Gel Digestgr, Kit™ (Millipore Corp.)
according to the manufacturer's protocol. Proteins of interest are identified using Matrixassisted
laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF MS) on
an Axima CFR MALDI-TOF Mass Spectrometer (Kratos Analytical, Inc.), as described in
Worrall, TA, et al., Anal, Chern. 70: 750 (1998), or using liquid chromatography.
[0070] The amount of recovery of the protein of interest can be adjusted by varying the
concentration and/or affinity of the antibody used in Example 1.
[0071] Example 4 - Covalent Linkage of Antibodies to Polystyrene Beads
[0072] Add 5mL of 12.5% (v/v) glutaraldehyde in 50 mM sodium phosphate, pH 7.0, to
about 25 hydrazide derivatized polystyrene beads (6.4mm diameter; Pierce Chemical
Company) and shake very gently for about 2 hours at room temperature. The beads are then
washed in a Buchner funnel (without filter paper) with lOOmL water and with 20 mL of 100
mM sodium phosphate buffer, pli 6.0. Next, the glutaraldehyde-activated beads arc placed in
an antibody solution prepared by dissolving 2.5mg Ab in 5mL of sodium phosphate buffer,
pH 6.0. After the beads are in the Ab solution, add 1 mg NaCNBH3 to the solution. Shake
gently overnight at room temperature. After shaking, the beads are washed with lOOmL of
100 mM sodium phosphate buffer, pH 6.0. and then washed with 50 mL of 100 rnM
NaHCO) The residual aldehydes on the beads are blocked by incubating the beads with 5mL
of 100 mM lysirie lor about 1 hour at room temperature. After incubating with lysine, the
beads are washed with lOOrnL of lOOmM Na2CO3 and with lOOmL water and dried. The Abcoated
beads can be stored dry at 4°C. The method above is disclosed in Tijssen, P., Practice
and Theory of Enzyme ImmunoAssays, in Burdon, RH and Knippenberg, PH, (Eds.),
Laboratory Techniques in Biocheinwtiy and Molecular Biology, Elsevier Science Publishers,
Vol. 15(1935).
[0073] While various embodiments of the present invention have been described herein, it
should be understood that these examples and embodiments have been presented by way of
example only, and not limitation. Thus, the breadth and scope of the present invention should
not be limited by any of the above-described exemplary embodiments.

We Claim:
1. A device for collecting blood comprising a reservoir and affinity molecules, wherein said affinity molecules are exposed to said blood during the time of collecting said blood, wherein said affinity molecules are completely contained within said reservoir and, wherein said affinity molecules are coated onto the interior of said reservoir.
2. The device as claimed in claim 1, wherein said affinity molecules are coated onto the interior of side walls of said reservoir.
3. The device as claimed in claim 2, wherein said interior side walls are ridged.
4. The device as claimed in claim 1, wherein said affinity molecules have an affinity towards undesired targets.
5. The device as claimed in claim 1, wherein said affinity molecules have an affinity towards desired targets.
6. The device as claimed in claim 1, wherein said affinity molecules are selected from the group consisting of antibodies, antibody fragments, enzymes, fragments of enzymes, enzyme substrates, fragments of enzyme substrates, nucleotides, oligonucleotides, polynucleotides, receptors, aptamers, nanobodies, fragments of receptors, ligands, fragments of enzymes, other proteins, amino acids, peptides, polypeptides, oligopeptides, saccharides, disaccharides, polysaccharides, glycoproteins, proteoglycans, and natural and synthetic polymers.
7. The device as claimed in claim 6, wherein said affinity molecules are antibodies or fragments thereof.
8. The device as claimed in claim 7, wherein said antibody or fragment thereof binds at least one protein within said blood.
9. The device as claimed in claim 8, wherein said at least one protein is selected from the group consisting of albumin, immunoglobulins, fibrinogen, protease inhibitors, antichymotrypsin, antithrombin, macroglobulins, inter-alpha-trypsin inhibitor, CI

inhibitor, plasmin inhibitor, heparin cofactor II, tranferrin, immunoglobulin M, haptoglobin complement and alpha-1 antitrypsin.
10. The device as claimed in claim 9, wherein said protein is albumin.

Documents:

3583-DELNP-2005-Abstract-(04-11-2008).pdf

3583-delnp-2005-abstract.pdf

3583-DELNP-2005-Assignment-(11-11-2008).pdf

3583-delnp-2005-assingment.pdf

3583-DELNP-2005-Claims-(04-11-2008).pdf

3583-delnp-2005-claims.pdf

3583-delnp-2005-correspodence-others.pdf

3583-DELNP-2005-Correspondence-Others(15-1-2008).pdf

3583-DELNP-2005-Correspondence-Others-(04-11-2008).pdf

3583-DELNP-2005-Correspondence-Others-(11-11-2008).pdf

3583-delnp-2005-correspondence-others-(22-12-2008).pdf

3583-DELNP-2005-Description (Complete)-(04-11-2008).pdf

3583-delnp-2005-description (complete).pdf

3583-DELNP-2005-Drawings-(04-11-2008).pdf

3583-delnp-2005-drawings.pdf

3583-DELNP-2005-Form-1-(11-11-2008).pdf

3583-delnp-2005-form-1.pdf

3583-delnp-2005-form-13-(11-11-2008).pdf

3583-delnp-2005-form-18.pdf

3583-DELNP-2005-Form-2-(04-11-2008).pdf

3583-delnp-2005-form-2.pdf

3583-DELNP-2005-Form-3(15-1-2008).pdf

3583-DELNP-2005-Form-3-(04-11-2008).pdf

3583-delnp-2005-form-3.pdf

3583-DELNP-2005-Form-5-(04-11-2008).pdf

3583-DELNP-2005-Form-5-(11-11-2008).pdf

3583-delnp-2005-form-5-(22-12-2008).pdf

3583-delnp-2005-form-5.pdf

3583-DELNP-2005-GPA-(04-11-2008).pdf

3583-delnp-2005-gpa-(22-12-2008).pdf

3583-delnp-2005-gpa.pdf

3583-delnp-2005-others-document-(22-12-2008).pdf

3583-delnp-2005-pct-304.pdf

3583-DELNP-2005-Petition-137(15-1-2008).pdf

3583-DELNP-2005-Petition-137-(04-11-2008).pdf

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Patent Number

233191

Indian Patent Application Number

3583/DELNP/2005

PG Journal Number

13/2009

Publication Date

27-Mar-2009

Grant Date

27-Mar-2009

Date of Filing

12-Aug-2005

Name of Patentee

BECTON, DICKINSON AND COMPANY

Applicant Address

1 BECTON DRIVE FRANKLIN LAKES NJ 07417 USA

Inventors:

#	Inventor's Name	Inventor's Address
1	ROSS, JULIE	15 WOOD DRIVE MORRIS PLAINS NJ 07950 USA.

PCT International Classification Number

B01L 3/14

PCT International Application Number

PCT/US2004/002019

PCT International Filing date

2004-02-13

PCT Conventions:

#	PCT Application Number	Date of Convention	Priority Country
1	60/447,014	2003-02-13	U.S.A.