| Title of Invention | NUCLEIC ACID SEQUENCES ENCODING ENANTIOSELECTIVE AMIDASES |
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| Abstract | The invention relates to a nucleic acid sequence encoding an enantioselective amidase with an amino acid sequence, which amino acid sequence has at least 70% identity with SEQ ID NO: 2. The invention also relates to a process for the fermentation, comprising a batch and a feed phase, of a microorganism in a fermentation medium, wherein the microorganism expresses a nucleic acid according to the invention and wherein between 0.5 and 50mg/l_fermentation medium Zn?2+¿ is fed during the fermentation. The invention also relates to a process for the preparation of an enantiomerically enriched carboxylic acid and/or an enantiomerically enriched carboxylic acid amide, in which a mixture of the corresponding D- and L-carboxylic acid amides is contacted with an expression product according to the invention in the presence of between 0.01mM and 100mM Zn?2+¿, whereby one of the enantiomers of the carboxylic acid amide is enantioselectively hydrolysed to form the corresponding enantiomerically enriched carboxylic acid, while the other enantiomer of the carboxylic acid amide remains unchanged. |
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0128-chenp-2004 description(complete).pdf
0128-chenp-2004 sequence listing.pdf
0128-chenp-2004 correspondence others.pdf
0128-chenp-2004 correspondence po.pdf
0128-chenp-2004 pct search report.pdf
0128-chenp-2004 power of attorney.pdf
| Patent Number | 234293 | ||||||||||||||||||
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| Indian Patent Application Number | 128/CHENP/2004 | ||||||||||||||||||
| PG Journal Number | 24/2009 | ||||||||||||||||||
| Publication Date | 12-Jun-2009 | ||||||||||||||||||
| Grant Date | 19-May-2009 | ||||||||||||||||||
| Date of Filing | 22-Jan-2004 | ||||||||||||||||||
| Name of Patentee | DSM IP Assets B.V | ||||||||||||||||||
| Applicant Address | Het Overloon 1, NL-6411 TE Heerlen, | ||||||||||||||||||
Inventors:
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| PCT International Classification Number | C12N 15/55 | ||||||||||||||||||
| PCT International Application Number | PCT/NL02/00471 | ||||||||||||||||||
| PCT International Filing date | 2002-07-15 | ||||||||||||||||||
PCT Conventions:
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