Title of Invention	METHOD FOR PRODUCING LACOSAMIDE
Abstract	The present invention relates to method of producing (R)-2- acetamido-N-benzyl-3-methoxypropionamide (lacosamide) comprising the O-methylation of a compound of formula I to produce a compound of formula II wherein Rx is an N-protecting group, characterised in that the O- methylation is being carried out in a one-step reaction and wherein the compound of formula II is obtained as an R-enantiomer of at least 88 % purity and the method is performed either (a) as a phase transfer catalysis or (b) by adding a methylation agent and an organo lithium compound to the compound of formula I.

Title of Invention

METHOD FOR PRODUCING LACOSAMIDE

Abstract

The present invention relates to method of producing (R)-2- acetamido-N-benzyl-3-methoxypropionamide (lacosamide) comprising the O-methylation of a compound of formula I to produce a compound of formula II wherein Rx is an N-protecting group, characterised in that the O- methylation is being carried out in a one-step reaction and wherein the compound of formula II is obtained as an R-enantiomer of at least 88 % purity and the method is performed either (a) as a phase transfer catalysis or (b) by adding a methylation agent and an organo lithium compound to the compound of formula I.

Full Text	Description (R)-2-acetamido-N-benzyl-3-methoxypropionamide (recommended INN: lacosamide) is an anticonvulsant drug useful for treating epilepsy and pain. Two methods for producing this compound are disclosed in US 6,048,899. Scheme 2 of US 6,048,899 comprises the benzylamide formation prior to the O-methylation. However, this reaction scheme results in various impurities which must be removed by chromatography which is impractical on an industrial scale. Also, the yield of the individual steps is only between 80 and 85%. Scheme 1 of US 6,048,899 involves the O-methylation of an N-protected D- serine prior to benzylamide formation, N-deprotection and N-acetylation. Although this production scheme is a more promising starting point for upscaling it suffers from major deficiencies. Most importantly, the O- methylation of N-protected D-serine using silver (I) oxide and methyliodide is impractical and expensive and results in partial racemisation (about 15%) which reduces the yield of this step to 79%. Also, the removal of the S- enantiomer during production of (R)-2-acetamido-N-benzyl-3- methoxypropionamide is extremely difficult. It has now been detected, surprisingly, that the racemisation can be avoided by using alternative O-methylation methods, such as e.g. O-methylation using phase transfer catalysis or O-methylation using organolithium and a suitable methylation agent, such as dimethyl sulfate. Also, the present invention provides an improved lacosamide synthesis route, wherein the O-methylation method is selective for the alcoholic hydroxy group of the N-protected D-serine. Accordingly, compared to the unspecific methylation suggested in scheme 1 of US 6,048,899, which also leads to the esterification of the carboxylic group, the present invention results in a shortened, more effective synthesis, wherein the subsequent step of hydrolysing the methyl ester group of an intermediate is avoided. Accordingly, the present invention relates to an improved method of producing (R)-2-acetamido-N-benzyl-3-methoxypropionamide comprising the O-methylation of a compound of formula I to produce a compound of formula II wherein Rx is an N-protecting group, characterised in that the O-methylation is being carried out in a one-step reaction and wherein racemisation is avoided such that the compound of formula II is obtained as an R-enantiomer of at least 88%, preferably at least 90% and even more preferred of at least 95, 96, 97, 98 or 99 % enantiomeric purity. The language "one-step reaction" as used in this patent application means that when transforming a compound of formula I to a compound of formula II no significant amount (i.e. an amount of 5 Mol% or more) of ester of the carboxyl group is formed that needs to be hydrolysed in a separate step. Usually, even less than 1 Mol% of ester is formed which is then removed during the further processing to lacosamide as described further below without the need for any additional hydrolysis step. The inventive O-methylation can be achieved by adding to a compound of formula I, such as e.g. to N-Boc-D-serine, a methylation agent in the presence of an organo metal compound, preferably an organo lithium compound. Suitable methylation agents are e.g. dimethyl sulphate, trimethyl phosphate or methyl iodide with dimethyl sulphate being particularly preferred. The organo lithium compound is preferably an alkyl lithium compound, such as butyllithium, methyllithium or hexyllithium or an aryllithium compound, such as phenyl lithium. More preferably the organo lithium compound is t-butyllithium or n-butyllithium and particularly preferred n-butyliithium. Alternatively, other organo metal compounds comprising a metal-carbon binding may be used, such as organo zinc compounds including organo zinc halides, organo aluminium compounds including organo aluminium halides, organo tin compounds including organo tin halides or/and organo magnesium compounds including organo magnesium halides (Grignard compounds), wherein halides include CI, Br or/and I. The organo moiety may be aryl or alkyl. Preferred are Grignard compounds Alkyl-Mg-Y, or Aryl-Mg-Y, wherein Y is CI, Br or I. THF/2-methoxyethyl ether mixtures, diethoxymethane or, preferably, THF may be used as solvent. The reaction is usually allowed to proceed for at least 5 hours at O-10°C, and preferably for 7-24 hours at O-10 °C, most preferably for 9-18 hours at O-5°C. Also, the reaction may be performed at higher or lower temperatures such as any temperature between -10 and +25°C if the reaction time is adapted accordingly. If the N-protecting group Rx of the compound of formula I is N-Boc a typical reaction can be illustrated by the following scheme (step 1-A) Surprisingly, this process does not result in methyl ester formation or significant racemisation of the product. The experimental yield is 91%, with the major impurities being N-methylations (e.g. Example 1). Therefore, the yield of the methylation according to the method of the present invention using an organo metal compound may be at least 85%, preferably at least 90%. Typically the amount of ester impurity after methylation using an organo metal or preferably an organo lithium compound, in particular after step 1a is significantly below 1 Mol%, preferably below 0,1 Mol% and is regularly below the limit of detection. In an alternative route the selective O-methylation of the alcoholic group of N-protected D-serine is performed by phase transfer catalysis ("PTC"). PTC is a method that makes use of heterogeneous two-phase systems - one phase being an aqueous or solid phase and a reservoir of reacting anions or a base for the generation of organic anions, whereas the organic reactants and catalysts are located in the second, organic phase. Usually, a quarternary ammonium, phosphonium or suifonium salt, such as e.g. a tetraalkylammonium halide, is used as phase transfer catalyst. Suitable catalysts and PTC reagents can be purchased from many vendors, e.g. from Sigma-Aldrich or Hawks Chemical. Accordingly, one embodiment of the present invention relates to a method of producing lacosamide, characterized in that a compound of formula I is O- methylated to a compound of formula II by performing the reaction as a phase transfer catalysis. Typically this method comprises the addition of a methylation reagent, such as dimethylsulfate, methyl iodide or trimethyl phosphate to a phase transfer reaction system comprising the compound of formula I, an aqueous phase, an organic phase and a phase transfer catalyst. In the PTC of the present invention preferably (a) the methylation agent is selected from dimethylsulfate, methyl iodide or trimethyl phosphate, wherein dimethylsulfate is particularly preferred; (b)the first (aqueous) phase is an alkaline aqueous solution, such as aqueous sodium hydroxide, aqueous lithium hydroxide, aqueous potassium hydroxide, aqueous sodium carbonate or aqueous potassium carbonate, wherein aqueous sodium hydroxide is particularly preferred; (c) the second (organic) phase is selected from toluene, hexane, methylene chloride or methyl t-butyl ether, with toluene being particularly preferred and (d) the phase transfer catalyst is an ammonium or phosphonium salt of formula IV, a sulfonium salt of formula V or a pyridinium salt of formula VI wherein R, R', R" and R'" are independently selectable alkyl, aryl or aralkyl groups, Q is a nitrogen or phosphorus and X is a halide, acetate, p-toluenesulfonate, trifluoromethanesulfonate, hexafiuoroantimonate, hydroxide, perchlorate, hydrogensulfate, thiocyanate or tetrafluoroborate. Examples of suitable phase transfer catalysts are tetraethylammonium p- toluenesulfonate, tetrapropylammonium trifluoromethanesulfonate, tetraphenylphosphonium hexafluoroantimonate, cetylpyridinium bromide, triphenylmethyl triphenyiphosponium chloride, benzyltriethylammonium chloride, benzyltrimethylammonium chloride, benzyltriphenylphosphonium chloride, benzytributylammonium chloride, butyltriethylammonium bromide, butyltriphenylphosphonium bromide, cetyltrimethyl ammonium bromide, cetyltrimethyl ammonium chloride, ethyltriphenylphosphonium bromide, ethyltriphenylphosphonium iodide, methyltrioctylammonium bromide, methyltriphenylphosphonium bromide, methyltriphenylphosphonium iodide, phenyltrimethylammonium chloride, tetrabutylammonium hydroxide, tetrabutylammonium perchlorate, tetrabutylammonium bromide, tetrabutylammonium hydrogensulphate, tetrabutylammonium iodide, tetrabutylammonium tetrafluoroborate, tetrabutylammonium thiocyanate, tetraethylammonium hydroxide, tetraethylammonium iodide, tetraethylammonium bromide, tetramethylammonium chloride, tetramethylammonium iodide, tetramethylammonium chloride, tetraoctylammonium bromide, tetraphenylphosphonium bromide, tetrapropylammonium hydroxide, tetrapropylammonium bromide and tributylmethylammonium chloride, wherein tetrabutylammonium salts and particularly tetrabutylammonium halides, e.g. the bromide are especially preferred. In the PTC of the present invention suitable concentrations of components (a)-(d) as defined above are as follows (a) the amount of methylation agent is 1 to 5 molar equivalents with respect to the compound of formula I (b) aqueous alkali is provided as a 5 to 50% w/w solution and in an amount of 1.1 to 10 molar equivalents with respect to the compound of formula I (c) the amount of organic solvent with respect to the compound of formula I is preferably between 3-20 volumes, in particular 3-20 l/kg compound of formula I (d) the amount of phase transfer catalyst is between 0.01 to 0.1 molar equivalents of the compound of formula I In the present invention, in particular in formula IV to VI "aryl" refers to an aromatic group, substituted with one ore more substituents or unsubstituted, which contains from 6 up to 18 ring carbon atoms and up to a total of 25 carbon atoms and includes polynuclear aromatics. These aryl groups may be monocyclic, bicyclic, tricyclic or polycyclic and may be fused rings. A polynuclear aromatic compound as used herein, is meant to encompass bicyclic fused aromatic ring systems containing from 1O-18 ring carbon atoms and up to a total of 25 carbon atoms. In the aryl group, one to six carbon atoms may be replaced by a heteroatom, such as oxygen, sulfur or/and nitrogen. "Aryl" comprises unsubstituted phenyl; unsubstituted naphtyl; phenyl or napthyl substituted with one or more substituents selected from e.g. hydroxy, carboxy, halogen, nitro, C1-6 alkyl, C1-6 alkoxy, amino; substituted or unsubstituted heteroaryls such as pyrroyl; thienyl, indolyl, etc. In the present invention, in particular in formula IV, "aryl" is preferably chosen from unsubstituted phenyl or substituted phenyl, e.g. 2,6- difluorophenyl, p-nitrophenyl or p-toluyl. Unsubstituted phenyl is particularly preferred. In the present invention, in particular in formula IV to VI "alkyl" comprises branched or linear saturated hydrocarbon chains. Preferably "alkyl" is a branched or linear hydrocarbon with up to 20 carbon atoms, more preferably with up to 6 carbon atoms; most preferably with up to 4 carbon atoms. The hydrocarbon may be substituted with one ore more substituents or unsubstituted. Preferred examples of "alkyl" are cetyl, octyl, heptyl, pentyl, butyl, propyl, ethyl and methyl. In the present invention, in particular in formula IV to VI, "aralkyl" means a group aryl-alkyl wherein "aryl and "alkyl" are as defined above. Preferably "aralkyl" is benzyl. In the present invention, substitution refers to substitution of a H atom by e.g. hydroxy, carboxy, halogen, nitro, C1-6 alkyl, C1-6 alkoxy, amino. The PTC reaction is usually allowed to proceed at O-10 °C for at least 30 minutes, e.g. for 0.5 to 24 hours, preferably for at least 45 minutes and even more preferred for at least 1 hour. The PTC of the present invention under the specific condition of e.g. Example 2 (step 1-B) yielded 96%. The yield of the PTC of the present invention may thus be at least 85%, preferably at least 90%, even more preferably at least 91%, 92%, 93%, 94%, 95% or 96%. A compound of formula I is obtainable from many vendors e.g. from Sigma- Aldrich or Lancaster. N-Boc-D-serine can also be produced by reacting D- serine with di-t-butyl dicarbonate to N-Boc-D-serine in a phase transfer catalysis reaction using essentially the conditions (e.g. choice and concentration/amount of alkali, solvent, PCT-catalysts, temperature, reaction time etc) as described above, except that di-t-butyl dicarbonate is used as the reagent instead of a methylation agent. If the N-protecting group of the compound of formula I is Boc (t- Butoxycarbonyl), a preferred PTC reaction can be illustrated by the following scheme (Step 1-B) This reaction does not result in any racemisation or esterification of the product. Also, the yield is further improved with impurity levels of only about 1%. Typically the amount of ester impurity after methyiation by PTC, in particular after step 1-B is well below 1 Mol%, preferably below 0,1 Mol%, and usually below the limit of detection. The process of the current invention may further comprise the step of processing the compound of formula II to a compound of formula III (Step 2) The benzyl amide formation can be performed by adding to a compound of formula II an amount of benzylamine in the presence of (a) a base such as triethylamine, diisopropylethylamine, 1,8-diazabicyclo [5.4.0]undec-7-ene, potassium bicarbonate or a morpholine derivative, preferably 4-methylmorpholine and (b) an activator of the carboxyl group such as a carbodiimide or an alkyl chloroformate, preferably isobutyl chloroformate. Experimentally, the yield of the benzylamid formation under the conditions of e.g. Example 3 (step 2) was typically between 90% and 99%. Thus, the yield of the benzylamid formation of the present invention may be in the range of at least 85% up to 99,9%, preferably in the range of at least 90% up to 99% product. This step 2 has been basically described in US 6,048,899, which is included herein by reference. Suitable protecting groups in the method according to the present invention are e.g. t-butoxycarbonyl (Boc) or carbobenzoxy (Cbz), with the Boc group being particularly preferred. In the compound of formula III the protecting group Rx can be cleaved off to obtain (R)-2-amino-N-benzyl-3-methoxypropionamide by appropriate measures known from the art. For example, if the protecting group Rx is a carbobenzoxy group it may be cleaved off with H2, Pd/C as described in US 6,048,899. If the protecting group is a Boc group this group may be conveniently removed with an acid, such as hydrochloric acid, e.g. at room temperature (step 3). Experimentally, the amine formation under the conditions of e.g. Example 3, step 3 typically yielded product in an amount ranging from 95% to 100%. Thus, the amine formation step in the method of the present invention may yield at least 85%, preferably at least 90%, more preferably at least 95% product. (R)-2-amino-N-benzyl-3-methoxypropionamide can then be transformed to lacosamide by N-acetylation using acetic anhydride (step 4) Experimentally, the acetylation under the conditions of e.g. Example 3, step 4 typically yielded between 81% and 95%. The yield of the acetylation step in the method of the present invention may thus be in the range of at least 70% up to 99%, preferably in the range of at least 80% up to 95%. This step has been also described in US 6,048,899. However, US 6,048,899 suggests the use of acetic anhydride in the presence of a base, e.g. pyridine. It has now been found unexpectedly that the pure (R)-enantiomer can be also obtained effectively if the toxic pyridine is removed from the reaction mixture. One embodiment of the present invention is thus the production of lacosamide comprising a step of N-acetylation of (R)-2-amino-N-benzyl-3- methoxypropionamide with acetic anhydride in the absence of a base, in particular in the absence of pyridine. The advantage of a base-free reaction is that toxic bases, such as pyridine, can be excluded. Finally, Iacosamide can be isolated from the reaction mixture of step 4 with improved purity by crystallisation in appropriate solvents, such as ethyl acetate. Experimentally, Iacosamide was obtained under the specific conditions of Examples 1 to 4 in a yield of typically 63%-70% (using butyllithium in step 1- A) or 66% to 75% (using PTC in step 1-B). Thus, Iacosamide may be obtained by the method of the present invention in a total yield ranging from at least 50% up to 90%, preferably from at least 60% up to 80%. If an organo metal compound is employed the total yield of Iacosamide may be more preferably in the range of at least 60% up to 70%, most preferably in the range of at least 63% up to 70%. If a PTC is employed, the total yield of Iacosamide may be more preferably in the range of at least 60% up to 75%, most preferably at least 66% up to 75%. The formation of a compound of formula II from a compound of formula I is comprised by the method of lacosamide synthesis as described above. Subject of the present invention is thus a method for production of a compound of formula (II) from a compound of formula (I) by O-methylation as described above essentially in the abscence of methyl ester formation or significant racemisation. D-serine derivatives or L-serine derivatives or mixtures of D- and L-serine derivatives in any ratio may be used in the method of the present invention. Due to the fact that during O-methylation of the alcoholic OH-group of D- or/and L-serine derivatives in the method of the present invention, there is essentially no methyl ester formation and no significant racemisation of the product, the method of the present invention for production of a compound of formula (II) or/and of lacosamide leads to an improved yield and an improved enantiomeric purity of the product. The invention also relates to important intermediates of the current process. The most important intermediate (R)-2-N-Boc-amino-3-methoxypropanoic acid (C-936) results from the improved O-methylation step according to the present invention (see figure 1). The compound can be easily isolated from the reaction mixture as the free acid or by forming a salt, such as e.g. a cyclohexylammonium salt. Suitable C-936 salts are also regarded to be part of the invention. Also, (R)-N-benzyl-2-N-Boc-amino-3-methoxypropionamide (C-937) which results from the benzyl amide formation (step 2 of figure 1) constitutes a part of the invention. Another aspect of the invention relates to the use of C-936 or C-937 or any salt thereof as an adduct or intermediate in a method of producing (R)-2- acetamido-N-benzyl-3-methoxypropionamide (lacosamide). Yet another aspect of the invention pertains to a method of producing a pharmaceutical formulation by the subsequent steps of (a) producing lacosamide by the method according to the present invention and (b) mixing lacosamide with pharmaceutically acceptable excipients. Another aspect of the present invention is a method of producing a compound of formula VIII comprising the O-methylation of a compound of formula VII, to produce a compound of formula VIII, wherein R4 is H, an N-protecting group, or/and a group having O-30 C atoms, and wherein R1 R2, and R3 are independently selected from H and groups having O-30 C atoms, characterized in that the O-methylation in being carried out in a one-step reaction and wherein the compound of formula VIII is obtained in the same configuration as the compound VII, of at least 88%, preferably of at least 90 %, more preferably of at least 95%, 96%, 97%, 98% or 99% enantiomeric purity. Preferably, R1, R2, and R3 are independently hydrogen, -OH, -SH, -NH2, -NO2, -CN, -COOH, -SOH, -SO2H, -SO3H, halogen, -OR10, -SR10, -NR10R11, -SOR10, -SO2R10, -SO3R10, substituted or unsubstituted alkyl as definied above, substituted or unsubstituted C2-C6-alkenyl, substituted or unsubstituted C2-C6-alkynyl, -(CO)-R10, -(CO)-O-R10, -O-(CO)-R10, substituted or unsubstituted aryl as defined above, substituted or unsubstituted C3-C13- hetaryl having 1-3 heteroatoms independently selected from N, S, O, substituted or unsubstituted aralkyl as defined above, substituted or unsubstituted C7-C15-alkaryl, substituted or unsubstituted C4-C14-hetaralkyl having 1-3 heteroatoms independently selected from N, S, O; substituted or unsubstituted C4-C14-alkhetaryl having 1-3 heteroatoms independently selected from N, S, O; or substituted or unsubstituted C3-C12-cycloalkyl having 0-3 heteroatoms independently selected from N, S, O. It is more preferred that R1 is H, R2 is H or/and R3 is H. It is most preferred that R1 is H, R2 is H and R3 is H. Preferably, R4 is selected from R1 and N-protecting groups. More preferably, R4 is the N-protecting group Rx as described above. Even more preferred is R1 being H, R2 being H, R3 being H and R4 being Rx as described above. In the substituents R4, R1, R2, R3, the groups R10 and R11 are independently hydrogen, substituted or unsubstituted alkyl as defined above, substituted or unsubstituted C2-C6-alkenyl, substituted or unsubstituted C2-C6-aIkynyl, substituted or unsubstituted aryl as defined above, substituted or unsubstituted C3-C13-hetaryl having 1-3 heteroatoms independently selected from N, S, O, substituted or unsubstituted aralkyl as defined above, substituted or unsubstituted C2-C15-alkaryl, substituted or unsubstituted C4- C14-hetaralkyl having 1-3 heteroatoms independently selected from N, S, O; substituted or unsubstituted C4-C14-alkhetaryl having 1-3 heteroatoms independently selected from N, S, O; or substituted or unsubstituted C3-C12- cycloalkyl having O-3 heteroatoms independently selected from N, S, O. Substitution in the groups R4, R1, R2, R3, R10 and R11 refers to substitution by one or more substituents as defined above, e.g. by hydroxy, carboxy, halogen, nitro, C1-C6 alkyl, C1-C6 alkoxy, amino, etc. "The same configuration" of the compound VIII with reference to compound VII means that essentially no racemisation takes place, or compound VIII is obtained in the same configuration as compound VII with an enantiomeric purity as defined above. If compound VII is in the R configuration, compound VIII is also in the R configuration. If compound VII is in the S configuration, compound VIII is also in the S configuration. It is preferred that compound VII is in the R configuration. The parameter of enantiomeric purity can be applied mutatis mutandis to enantiomer mixtures. If compound VII is a mixture of the R and S configuration, compound VIII is essentially the same mixture of the R and S configuration, i.e. the ratio of the R and S configuration remains essentially unaltered, or an enantiomeric ratio as follows is obtained. The obtained enantiomeric ratio of compound VIII may be at least 88%, preferably at least 90 %, more preferably in at least 95, 96, 97, 98 or 99% of the enantiomeric ratio of compound VII. "One-step reaction" has the same meaning as discussed above. The reaction schema of compound VII to compound VIII is a generalization of the O-methylation of the present invention of the compound of formula I to produce a compound of formula II as described above. If the compound VII is in the R configuration, R1 is H, R2 is H, R3 is H and R4 is Rx, the compound VIII corresponds to the compound of formula II and may be used for the production of lacosamide, e.g. by the reaction steps as described above. Starting from the compound II or VII, lacosamide may be produced by any suitable method to introduce the N-benzylamide group and the N-acetyl group. Therefore, in a particular preferred embodiment, the compound VII is in the R configuration and R4 is Rx. It is most preferred that R4 is Rx, R1 is H, R2 is H, and R3 is H and the compound VII is in the R configuration. The inventive O-methylation of compound VII can be achieved by adding to a compound of formula VII a methylation agent in the presence of an organo metal compound, in particular an organo lithium compound, as defined above. Suitable methylation agents are defined above. In an alternative route, the selective O-methylation of the alcoholic group may be performed by phase transfer catalysis as defined above. Specific embodiments of the O-methylation of the compound of formula VII correspond to the specific embodiments of the production method of lacosamide comprising the O-methylation of the compound of formula I as described above, in particular specific embodiments relating to the phase transfer catalysis, phase transfer catalysts, in particular as defined in formula IV, V, and VI, the phase transfer reaction system and its components, the organo metal compound, reaction conditions during phase transfer catalysis or in the presence of the organo metal compound, further reaction steps and reaction conditions leading to lacosamide including N-benzylamide formation, N-deprotection and N-acetylation, etc. The yield of the methylation of compound VII by the method of the present invention may be at least 85%, preferably at least 90% when using an organo metal compound. When using PTC, the yield of methylation of compound VII may be at least 85%, preferably at least 90%, even more preferably at least 91%, 92%, 93%, 94%, 95 % or 96%. Due to the fact that during O-methylation of D- or/and L-serine derivatives in the method of the present invention, there is essentially no methyl ester formation and no significant racemisation of the product, the method of the present invention for production of a compound of formula VIII by methylation of a compound of formula VII leads to an improved yield and an improved enantiomeric purity of the product. The invention is further illustrated by Figure 1 (comprising alternative steps 1 a or 1 b) and the following Examples: Example 1: Production of (R)-2-N-Boc-amino-3-methoxypropanoic acid (C-936) using butyllithium (step 1a) A solution of N-Boc-D-serine (22g, 0.107 mol) in dry tetrahydrofuran (352ml) was cooled to dry addition funnel 15% w/w n-butyllithium in hexanes (134ml, 0.216mol) keeping the temperature 5'C. Dimethyl sulphate (12.1ml, 0.128mol) was added keeping the temperature at O-5'C and the reaction mixture aged at O-5'C for 9 hours. The reaction was quenched by the addition of water (110ml), basified to pH 10-13 with 30% sodium hydroxide (3ml) and the tetrahydrofuran/hexane evaporated in vacuo. The residue was washed with toluene (44ml) and then acidified to a pH of with methylene chloride (2x91ml, 1x66ml) and the combined C936 extracts dried by azeotropic distillation. Yield on evaporation 23.7g, 100%. HPLC purity 90.0%, Chiral purity 100%. Example 2: Production of (R)-2-N-Boc-amino-3-methoxypropanoic acid (C-936) using PTC (step 1b) A suspension of N-Boc-D-serine (22g, 0.107 mol) and tetrabutylammonium bromide (1.3g, 0.004mol) in toluene (110ml) was cooled to was added 20% sodium hydroxide (17.6ml, 0.107mol) keeping the temperature (25.4ml, 0.485mol) were added keeping the temperature reaction mixture aged at 10"C for 1 hour. Water (66ml) was added to the mixture and the phases separated. The aqueous layer was acidified to a pH of and the combined C936 extracts dried by azeotropic distillation. (Yield on evaporation 27.5g, 100%, HPLC purity 96.3%, Chiral purity 98.1%) Example 3: Steps 2 to 4 (R)-N-benzyl-2-N-Boc-amino-3-methoxypropionamide (C-937) solution (step 2) The C936 solution prepared as above in example 2 was cooled to and isobutyl chloroformate (14.2ml, 0.107mol) at (11.8ml, 0.17mol) was added at added at hour the mixture was washed with water (44ml), 1N HCI (44ml), 8% sodium bicarbonate (44ml) and water (44ml) to yield a C937 solution in methylene chloride. (R)-2-amino-N-benzyl-3-methoxypropionamide solution (step 3) To the C937 solution prepared above was added 36% HCI (46.5ml, 0.541 mol) and the mixture aged for 1 hour. Water (66ml) was added and the phases separated. The organic phase was extracted with water (22ml) and the aqueous layers combined. The aqueous was basified to pH 1O-12 with 30% sodium hydroxide at aqueous layer was extracted with methylene chloride (2x110ml) and the combined organic layers washed with water (44ml) yielding a methylene chloride solution of (R)-2-amino-N-benzyl-3-methoxypropionamide. Lacosamide (step 4) The (R)-2-amino-N-benzyl-3-methoxypropionamide solution prepared above was cooled to The reaction mixture is warmed to room temperature over 30 minutes and aged for a further 30 minutes. The mixture is then washed with water (44ml), 8% sodium bicarbonate (44ml) and water (44ml). The methylene chloride was exchanged for ethyl acetate by distillation and the solution distilled to a volume of 115ml. The product was crystallised by cooling the solution to O-5'C and the pure Lacosamide isolated by filtration (18.7g, 69.8%) HPLC purity 99.98%, Chiral purity 99.8% ee. Example 4: Isolation of (R)-2-N-Boc-amino-3-methoxypropanoic acid (C-936) The (R)-2-N-Boc-amino-3-rnethoxypropanoic acid (C-936) solution prepared in example 1 was evaporated in vacuo yielding (R)-2-N-Boc-amino-3- methoxypropanoic acid (C-936) as a waxy solid (23.7g, 100%). HPLC purity 90.0%. Elemental analysis Calculated for C9H17NO5 49.31% C; 7.82% H; 6.39% N. Found 49.12% C; 7.72% H; 8.97% N. Example 5: Isolation of (R)-N-benzyl-2-N-Boc-amino-3- methoxypropionamide (C-937) The (R)-N-benzyl-2-N-Boc-amino-3-methoxypropionamide (C-937) solution prepared in example 3 above was evaporated in vacuo yielding crude C937 as an oily solid. The crude solid (2g) was dissolved in 10% chloroform in hexane (30ml) at 60'C, cooled to room temperature and left stand for 1 hour at this temperature. The resulting solids were isolated by filtration yielding crude C937 (1.1g). This crude solid was further recrystallised twice in 10 volumes of 10% chloroform in hexane to yield (R)-N-benzyl-2-N-Boc-amino-3- methoxypropionamide (C-937) as a white crystalline solid (0.28g, 14%). HPLC purity 97.3%. Elemental analysis Calculated for C16H24N2O5 62.32% C; 7.84% H; 9.08% N. Found 62.19% C; 7.79% H; 9.04% N. WE CLAIM: 1. Method of producing (R)-2-acetamido-N-benzyl-3- methoxypropionamide (lacosamide) comprising the O-methylation of a compound of formula I to produce a compound of formula II wherein Rx is an N-protecting group, characterised in that the O-methylation is being carried out in a one-step reaction and wherein the compound of formula II is obtained as an R-enantiomer of at least 88 % purity and the method is performed either (a) as a phase transfer catalysis or (b) by adding a methylation agent and an organo lithium compound to the compound of formula I. 2. Method as claimed in claim 1, wherein the method comprises the addition of a methylation agent to a phase transfer reaction system comprising the compound of formula I, an aqueous phase, an organic phase and a phase transfer catalyst. 3. Method as claimed in claim 2, wherein a pyridinium, phosphonium, ammonium or sulfonium salt is used as a phase transfer catalyst. 4. Method as claimed in anyone of claims 2-3, wherein the phase transfer catalyst is chosen from compounds of (a) general formula IV (b) general formula V or (c) general formula VI wherein R, R', R" and R"', if present, are independently selectable alkyl, aryl or aralkyl groups; Q, in compounds of formula IV, is a nitrogen or phosphorus; and X is a halide, acetate, p-toluenesulfonate, trifluoromethanesulfonate, hexafluoroantimonate, hydroxide, perchlorate, hydrogensulfate, thiocyanate or tetrafluoroborate group. 5. Method as claimed in anyone of claims 2-4, wherein the phase transfer catalyst is tetrabutylammonium bromide. 6. Method as claimed in anyone of claims 2-5, wherein the methylating agent used in the phase transfer catalysis is chosen from dimethyl sulphate, trimethyl phosphate or methyl iodide. 7. Method as claimed in anyone of claims 2-6, wherein the aqueous phase is aqueous sodium hydroxide, aqueous lithium hydroxide, aqueous potassium hydroxide, aqueous sodium carbonate or aqueous potassium carbonate. 8. Method as claimed in anyone of claims 2-7, wherein the organic solvent is toluene, hexane, methylene chloride or methyl t-butyl ether. 9. Method as claimed in anyone of claims 1-8, wherein the phase transfer catalysis is performed at O-10 °C for at least 30 minutes. 10. Method as claimed in claim 1, wherein the methylation agent used with the organo lithium compound is dimethylsulfate. 11. Method as claimed in anyone of claims 1 or 10, wherein the organo lithium compound is butyl lithium. 12. Method as claimed in claims 1 or 10 to 11, wherein the O- methylation in the presence of an organo lithium compound takes place at a temperature of O-10°C for at least 5 hrs. 13. Method as claimed in anyone of the preceding claims further comprising the reaction of compound II with benzylamine to give the compound of formula III, and then replacing the protecting group Rx with methyl carbonyl to give (R)-2-acetamido-N-benzyl-3-methoxypropionamide (lacosamide). 14. Method as claimed in claim 13, wherein the reaction of the compound of formula II with benzylamine takes place in the presence of an activator of the carboxyl group and a base. 15. Method as claimed in claim 14, wherein the base is 4- methylmorpholine, triethylamine, diisopropylethylamine, 1.8- diazabycyclo[5.4.0]undec-7-ene or potassium bicarbonate and the activator of the carboxyl group is an alkyl chloroformate or a carbodiimide. 16. Method as claimed in anyone of claims 13-15, wherein the N- protecting group Rx is replaced by methyl carbonyl by successively (a) cleaving off the protecting group Rx from the compound of formula III by the addition of (i) a mineralic acid or (ii) H2/Pd-C to yield (R)-2- amino-N-benzyl-3-methoxypropionamide and then (b) adding the methyl carbonyl group to (R)-2-amino-N-benzyl-3-methoxyprop ionamide by the reaction of (R)-2-amino-N-benzyl-3- methoxypropionamide with acetic anhydride. 17. Method as claimed in claim 16, wherein step (b) is performed in the absence of pyridine. 18. Method as claimed in anyone of claims 13-15 wherein the N- protecting group Rx is cleaved off to obtain (R)-2-amino-N-benzyl-3- methoxypropionamide. 19. Method as claimed in anyone of the preceding claims wherein lacosamide is isolated from the final reaction mix by crystallisation. 20. Method as claimed in anyone of the preceding claims wherein the N-protecting group is t-butyloxy carbonyl (Boc). 21. Method as claimed in any of the preceding claims comprising the step of N-acetylation of (R)-2-amino-N-benzyl-3-methoxypropionamide with acetic anhydride in the absence of a base, in particular in the absence of pyridine. 22. Method as claimed in any one of the preceding claims wherein the compound of formula II is (R)-2-N-Boc-amino-3-methoxypropanoic acid (C-936) or salts thereof. 23. Method as claimed in any one of claims 13-18, wherein the compound of formula III is (R)-N-benzyl-2-N-Boc-amino-3- methoxypropionamide (C-937) or any salt thereof. 24. Method of producing a pharmaceutical formulation comprising lacosamide by the subsequent steps of (a) producing lacosamide by anyone of claims 1 to 22 and (b) mixing lacosamide with pharmaceutical acceptable excipients. 25. Method as claimed in anyone of claims 1 to 22 further comprising production of N-Boc-D-serine by reacting D-serine with di-t-butyl dicarbonate in a phase transfer reaction. 26. Method of producing a compound of formula VIII comprising the O-methylation of a compound of formula VII, to produce a compound of formula VIII, wherein R1, R2 and R3 are independently selected from hydrogen, -OH, -SH, -NH2, -NO2, -CN, -COOH, -SOH, -SO2H, -SO3H, halogen, -OR10, - SR10, -NR10R11, -SOR10, -SO2R10, -SO3R10, substituted or unsubstituted alkyl, substituted or unsubstituted C2-C6-alkenyl, substituted or unsubstituted C2-C6-alkynyl, -(CO)-R10, -(CO)-O-R10, - O-(CO)-R10, substituted or unsubstituted aryl, substituted or unsubstituted C3-C13-hetaryl having 1-3 heteroatoms independently selected from N, S, O, substituted or unsubstituted aralkyl, substituted or unsubstituted C7-C15-alkaryl, substituted or unsubstituted C4-C14-hetaralkyl having 1-3 heteroatoms independently selected from N, S, O; substituted or unsubstituted C4- C14-alkhetaryl having 1-3 heteroatoms independently selected from N, S, O; and substituted or unsubstituted C3-C12-cycloalkyl having O-3 heteroatoms independently selected from N, S, O, wherein R4 is selected from R1 and N-protecting groups and wherein R10 and R11 are independently selected from hydrogen, substituted or unsubstituted alkyl, substituted or unsubstituted C2-C6-alkenyl, substituted or unsubstituted C2-C6-alkynyl, substituted or unsubstituted aryl, substituted or unsubstituted C3-C13-hetaryl having 1-3 heteroatoms independently selected from N, S, O, substituted or unsubstituted aralkyl, substituted or unsubstituted C7- C15-alkaryl, substituted or unsubstituted C4-C14-hetaralkyl having 1-3 heteroatoms independently selected from N, S, O; substituted or unsubstituted C4-C14-alkhetaryl having 1-3 heteroatoms independently selected from N, S, O; and substituted or unsubstituted C3-C12-cycloalkyl having O-3 heteroatoms independently selected from N, S, O, characterised in that the O-methylation is being carried out in a one-step reaction wherein the compound of formula VIII is obtained in the same configuration as the compound VII in at least 88 % purity and the method is performed either (a) as a phase transfer catalysis or (b) by adding a methylation agent and an organo lithium compound to the compound of formula VII. 27. Method as claimed in claim 26, wherein R1 is H, R2 is H and R3 is H and R4 is a N-protecting group. 28. Method as claimed in any of claims 26 or 27, wherein compound VII is in the R-configuration. ABSTRACT TITLE: METHOD FOR PRODUCING LACOSAMIDE The present invention relates to method of producing (R)-2- acetamido-N-benzyl-3-methoxypropionamide (lacosamide) comprising the O-methylation of a compound of formula I to produce a compound of formula II wherein Rx is an N-protecting group, characterised in that the O- methylation is being carried out in a one-step reaction and wherein the compound of formula II is obtained as an R-enantiomer of at least 88 % purity and the method is performed either (a) as a phase transfer catalysis or (b) by adding a methylation agent and an organo lithium compound to the compound of formula I.

Full Text

Description
(R)-2-acetamido-N-benzyl-3-methoxypropionamide (recommended INN:
lacosamide) is an anticonvulsant drug useful for treating epilepsy and pain.
Two methods for producing this compound are disclosed in US 6,048,899.
Scheme 2 of US 6,048,899 comprises the benzylamide formation prior to the
O-methylation. However, this reaction scheme results in various impurities
which must be removed by chromatography which is impractical on an
industrial scale. Also, the yield of the individual steps is only between 80 and
85%.
Scheme 1 of US 6,048,899 involves the O-methylation of an N-protected D-
serine prior to benzylamide formation, N-deprotection and N-acetylation.
Although this production scheme is a more promising starting point for
upscaling it suffers from major deficiencies. Most importantly, the O-
methylation of N-protected D-serine using silver (I) oxide and methyliodide is
impractical and expensive and results in partial racemisation (about 15%)
which reduces the yield of this step to 79%. Also, the removal of the S-
enantiomer during production of (R)-2-acetamido-N-benzyl-3-
methoxypropionamide is extremely difficult.
It has now been detected, surprisingly, that the racemisation can be avoided
by using alternative O-methylation methods, such as e.g. O-methylation
using phase transfer catalysis or O-methylation using organolithium and a
suitable methylation agent, such as dimethyl sulfate.
Also, the present invention provides an improved lacosamide synthesis
route, wherein the O-methylation method is selective for the alcoholic
hydroxy group of the N-protected D-serine. Accordingly, compared to the
unspecific methylation suggested in scheme 1 of US 6,048,899, which also

leads to the esterification of the carboxylic group, the present invention
results in a shortened, more effective synthesis, wherein the subsequent
step of hydrolysing the methyl ester group of an intermediate is avoided.
Accordingly, the present invention relates to an improved method of
producing (R)-2-acetamido-N-benzyl-3-methoxypropionamide comprising
the O-methylation of a compound of formula I

to produce a compound of formula II

wherein Rx is an N-protecting group,
characterised in that the O-methylation is being carried out in a one-step
reaction and wherein racemisation is avoided such that the compound of
formula II is obtained as an R-enantiomer of at least 88%, preferably at least
90% and even more preferred of at least 95, 96, 97, 98 or 99 %
enantiomeric purity.
The language "one-step reaction" as used in this patent application means
that when transforming a compound of formula I to a compound of formula II
no significant amount (i.e. an amount of 5 Mol% or more) of ester of the
carboxyl group is formed that needs to be hydrolysed in a separate step.
Usually, even less than 1 Mol% of ester is formed which is then removed
during the further processing to lacosamide as described further below
without the need for any additional hydrolysis step.

The inventive O-methylation can be achieved by adding to a compound of
formula I, such as e.g. to N-Boc-D-serine, a methylation agent in the
presence of an organo metal compound, preferably an organo lithium
compound. Suitable methylation agents are e.g. dimethyl sulphate, trimethyl
phosphate or methyl iodide with dimethyl sulphate being particularly
preferred. The organo lithium compound is preferably an alkyl lithium
compound, such as butyllithium, methyllithium or hexyllithium or an
aryllithium compound, such as phenyl lithium. More preferably the organo
lithium compound is t-butyllithium or n-butyllithium and particularly preferred
n-butyliithium. Alternatively, other organo metal compounds comprising a
metal-carbon binding may be used, such as organo zinc compounds
including organo zinc halides, organo aluminium compounds including
organo aluminium halides, organo tin compounds including organo tin
halides or/and organo magnesium compounds including organo magnesium
halides (Grignard compounds), wherein halides include CI, Br or/and I. The
organo moiety may be aryl or alkyl. Preferred are Grignard compounds
Alkyl-Mg-Y, or Aryl-Mg-Y, wherein Y is CI, Br or I. THF/2-methoxyethyl ether
mixtures, diethoxymethane or, preferably, THF may be used as solvent. The
reaction is usually allowed to proceed for at least 5 hours at O-10°C, and
preferably for 7-24 hours at O-10 °C, most preferably for 9-18 hours at O-5°C.
Also, the reaction may be performed at higher or lower temperatures such
as any temperature between -10 and +25°C if the reaction time is adapted
accordingly.
If the N-protecting group Rx of the compound of formula I is N-Boc a typical
reaction can be illustrated by the following scheme (step 1-A)

Surprisingly, this process does not result in methyl ester formation or
significant racemisation of the product. The experimental yield is 91%, with
the major impurities being N-methylations (e.g. Example 1). Therefore, the
yield of the methylation according to the method of the present invention
using an organo metal compound may be at least 85%, preferably at least
90%.
Typically the amount of ester impurity after methylation using an organo
metal or preferably an organo lithium compound, in particular after step 1a is
significantly below 1 Mol%, preferably below 0,1 Mol% and is regularly below
the limit of detection.
In an alternative route the selective O-methylation of the alcoholic group of
N-protected D-serine is performed by phase transfer catalysis ("PTC"). PTC
is a method that makes use of heterogeneous two-phase systems - one
phase being an aqueous or solid phase and a reservoir of reacting anions or
a base for the generation of organic anions, whereas the organic reactants
and catalysts are located in the second, organic phase.
Usually, a quarternary ammonium, phosphonium or suifonium salt, such as
e.g. a tetraalkylammonium halide, is used as phase transfer catalyst.
Suitable catalysts and PTC reagents can be purchased from many vendors,
e.g. from Sigma-Aldrich or Hawks Chemical.
Accordingly, one embodiment of the present invention relates to a method of
producing lacosamide, characterized in that a compound of formula I is O-
methylated to a compound of formula II by performing the reaction as a
phase transfer catalysis.
Typically this method comprises the addition of a methylation reagent, such
as dimethylsulfate, methyl iodide or trimethyl phosphate to a phase transfer
reaction system comprising the compound of formula I, an aqueous phase,
an organic phase and a phase transfer catalyst.

In the PTC of the present invention preferably
(a) the methylation agent is selected from dimethylsulfate, methyl iodide or
trimethyl phosphate, wherein dimethylsulfate is particularly preferred;
(b)the first (aqueous) phase is an alkaline aqueous solution, such as
aqueous sodium hydroxide, aqueous lithium hydroxide, aqueous
potassium hydroxide, aqueous sodium carbonate or aqueous potassium
carbonate, wherein aqueous sodium hydroxide is particularly preferred;
(c) the second (organic) phase is selected from toluene, hexane, methylene
chloride or methyl t-butyl ether, with toluene being particularly preferred
and
(d) the phase transfer catalyst is an ammonium or phosphonium salt of
formula IV, a sulfonium salt of formula V or a pyridinium salt of formula VI

wherein R, R', R" and R'" are independently selectable alkyl, aryl or
aralkyl groups, Q is a nitrogen or phosphorus and X is a halide,
acetate, p-toluenesulfonate, trifluoromethanesulfonate,
hexafiuoroantimonate, hydroxide, perchlorate, hydrogensulfate,

thiocyanate or tetrafluoroborate.
Examples of suitable phase transfer catalysts are tetraethylammonium p-
toluenesulfonate, tetrapropylammonium trifluoromethanesulfonate,
tetraphenylphosphonium hexafluoroantimonate, cetylpyridinium bromide,
triphenylmethyl triphenyiphosponium chloride, benzyltriethylammonium
chloride, benzyltrimethylammonium chloride, benzyltriphenylphosphonium
chloride, benzytributylammonium chloride, butyltriethylammonium bromide,
butyltriphenylphosphonium bromide, cetyltrimethyl ammonium bromide,
cetyltrimethyl ammonium chloride, ethyltriphenylphosphonium bromide,
ethyltriphenylphosphonium iodide, methyltrioctylammonium bromide,
methyltriphenylphosphonium bromide, methyltriphenylphosphonium iodide,
phenyltrimethylammonium chloride, tetrabutylammonium hydroxide,
tetrabutylammonium perchlorate, tetrabutylammonium bromide,
tetrabutylammonium hydrogensulphate, tetrabutylammonium iodide,
tetrabutylammonium tetrafluoroborate, tetrabutylammonium thiocyanate,
tetraethylammonium hydroxide, tetraethylammonium iodide,
tetraethylammonium bromide, tetramethylammonium chloride,
tetramethylammonium iodide, tetramethylammonium chloride,
tetraoctylammonium bromide, tetraphenylphosphonium bromide,
tetrapropylammonium hydroxide, tetrapropylammonium bromide and
tributylmethylammonium chloride, wherein tetrabutylammonium salts and
particularly tetrabutylammonium halides, e.g. the bromide are especially
preferred.
In the PTC of the present invention suitable concentrations of components
(a)-(d) as defined above are as follows
(a) the amount of methylation agent is 1 to 5 molar equivalents with respect
to the compound of formula I
(b) aqueous alkali is provided as a 5 to 50% w/w solution and in an amount
of 1.1 to 10 molar equivalents with respect to the compound of formula I
(c) the amount of organic solvent with respect to the compound of formula I

is preferably between 3-20 volumes, in particular 3-20 l/kg compound of
formula I
(d) the amount of phase transfer catalyst is between 0.01 to 0.1 molar
equivalents of the compound of formula I
In the present invention, in particular in formula IV to VI "aryl" refers to an
aromatic group, substituted with one ore more substituents or unsubstituted,
which contains from 6 up to 18 ring carbon atoms and up to a total of 25
carbon atoms and includes polynuclear aromatics. These aryl groups may
be monocyclic, bicyclic, tricyclic or polycyclic and may be fused rings. A
polynuclear aromatic compound as used herein, is meant to encompass
bicyclic fused aromatic ring systems containing from 1O-18 ring carbon
atoms and up to a total of 25 carbon atoms. In the aryl group, one to six
carbon atoms may be replaced by a heteroatom, such as oxygen, sulfur
or/and nitrogen. "Aryl" comprises unsubstituted phenyl; unsubstituted
naphtyl; phenyl or napthyl substituted with one or more substituents selected
from e.g. hydroxy, carboxy, halogen, nitro, C1-6 alkyl, C1-6 alkoxy, amino;
substituted or unsubstituted heteroaryls such as pyrroyl; thienyl, indolyl, etc.
In the present invention, in particular in formula IV, "aryl" is preferably
chosen from unsubstituted phenyl or substituted phenyl, e.g. 2,6-
difluorophenyl, p-nitrophenyl or p-toluyl. Unsubstituted phenyl is particularly
preferred.
In the present invention, in particular in formula IV to VI "alkyl" comprises
branched or linear saturated hydrocarbon chains. Preferably "alkyl" is a
branched or linear hydrocarbon with up to 20 carbon atoms, more preferably
with up to 6 carbon atoms; most preferably with up to 4 carbon atoms. The
hydrocarbon may be substituted with one ore more substituents or
unsubstituted. Preferred examples of "alkyl" are cetyl, octyl, heptyl, pentyl,
butyl, propyl, ethyl and methyl.

In the present invention, in particular in formula IV to VI, "aralkyl" means a
group aryl-alkyl wherein "aryl and "alkyl" are as defined above. Preferably
"aralkyl" is benzyl.
In the present invention, substitution refers to substitution of a H atom by
e.g. hydroxy, carboxy, halogen, nitro, C1-6 alkyl, C1-6 alkoxy, amino.
The PTC reaction is usually allowed to proceed at O-10 °C for at least 30
minutes, e.g. for 0.5 to 24 hours, preferably for at least 45 minutes and even
more preferred for at least 1 hour.
The PTC of the present invention under the specific condition of e.g.
Example 2 (step 1-B) yielded 96%. The yield of the PTC of the present
invention may thus be at least 85%, preferably at least 90%, even more
preferably at least 91%, 92%, 93%, 94%, 95% or 96%.
A compound of formula I is obtainable from many vendors e.g. from Sigma-
Aldrich or Lancaster. N-Boc-D-serine can also be produced by reacting D-
serine with di-t-butyl dicarbonate to N-Boc-D-serine in a phase transfer
catalysis reaction using essentially the conditions (e.g. choice and
concentration/amount of alkali, solvent, PCT-catalysts, temperature, reaction
time etc) as described above, except that di-t-butyl dicarbonate is used as
the reagent instead of a methylation agent.
If the N-protecting group of the compound of formula I is Boc (t-
Butoxycarbonyl), a preferred PTC reaction can be illustrated by the following
scheme (Step 1-B)

This reaction does not result in any racemisation or esterification of the
product. Also, the yield is further improved with impurity levels of only about
1%.
Typically the amount of ester impurity after methyiation by PTC, in particular
after step 1-B is well below 1 Mol%, preferably below 0,1 Mol%, and usually
below the limit of detection.
The process of the current invention may further comprise the step of
processing the compound of formula II to a compound of formula III (Step 2)

The benzyl amide formation can be performed by adding to a compound of
formula II an amount of benzylamine in the presence of
(a) a base such as triethylamine, diisopropylethylamine, 1,8-diazabicyclo
[5.4.0]undec-7-ene, potassium bicarbonate or a morpholine derivative,
preferably 4-methylmorpholine and
(b) an activator of the carboxyl group such as a carbodiimide or an alkyl
chloroformate, preferably isobutyl chloroformate.
Experimentally, the yield of the benzylamid formation under the conditions of
e.g. Example 3 (step 2) was typically between 90% and 99%. Thus, the yield
of the benzylamid formation of the present invention may be in the range of
at least 85% up to 99,9%, preferably in the range of at least 90% up to 99%
product.
This step 2 has been basically described in US 6,048,899, which is included
herein by reference.

Suitable protecting groups in the method according to the present invention
are e.g. t-butoxycarbonyl (Boc) or carbobenzoxy (Cbz), with the Boc group
being particularly preferred.
In the compound of formula III the protecting group Rx can be cleaved off to
obtain (R)-2-amino-N-benzyl-3-methoxypropionamide by appropriate
measures known from the art. For example, if the protecting group Rx is a
carbobenzoxy group it may be cleaved off with H2, Pd/C as described in US
6,048,899. If the protecting group is a Boc group this group may be
conveniently removed with an acid, such as hydrochloric acid, e.g. at room
temperature (step 3).

Experimentally, the amine formation under the conditions of e.g. Example 3,
step 3 typically yielded product in an amount ranging from 95% to 100%.
Thus, the amine formation step in the method of the present invention may
yield at least 85%, preferably at least 90%, more preferably at least 95%
product.
(R)-2-amino-N-benzyl-3-methoxypropionamide can then be transformed to
lacosamide by N-acetylation using acetic anhydride (step 4)

Experimentally, the acetylation under the conditions of e.g. Example 3, step
4 typically yielded between 81% and 95%. The yield of the acetylation step
in the method of the present invention may thus be in the range of at least
70% up to 99%, preferably in the range of at least 80% up to 95%.
This step has been also described in US 6,048,899. However, US 6,048,899
suggests the use of acetic anhydride in the presence of a base, e.g.
pyridine. It has now been found unexpectedly that the pure (R)-enantiomer
can be also obtained effectively if the toxic pyridine is removed from the
reaction mixture.
One embodiment of the present invention is thus the production of
lacosamide comprising a step of N-acetylation of (R)-2-amino-N-benzyl-3-
methoxypropionamide with acetic anhydride in the absence of a base, in
particular in the absence of pyridine. The advantage of a base-free reaction
is that toxic bases, such as pyridine, can be excluded.
Finally, Iacosamide can be isolated from the reaction mixture of step 4 with
improved purity by crystallisation in appropriate solvents, such as ethyl
acetate.
Experimentally, Iacosamide was obtained under the specific conditions of
Examples 1 to 4 in a yield of typically 63%-70% (using butyllithium in step 1-
A) or 66% to 75% (using PTC in step 1-B). Thus, Iacosamide may be
obtained by the method of the present invention in a total yield ranging from
at least 50% up to 90%, preferably from at least 60% up to 80%. If an
organo metal compound is employed the total yield of Iacosamide may be
more preferably in the range of at least 60% up to 70%, most preferably in
the range of at least 63% up to 70%. If a PTC is employed, the total yield of
Iacosamide may be more preferably in the range of at least 60% up to 75%,
most preferably at least 66% up to 75%.
The formation of a compound of formula II from a compound of formula I is

comprised by the method of lacosamide synthesis as described above.
Subject of the present invention is thus a method for production of a
compound of formula (II) from a compound of formula (I) by O-methylation
as described above essentially in the abscence of methyl ester formation or
significant racemisation.
D-serine derivatives or L-serine derivatives or mixtures of D- and L-serine
derivatives in any ratio may be used in the method of the present invention.
Due to the fact that during O-methylation of the alcoholic OH-group of D-
or/and L-serine derivatives in the method of the present invention, there is
essentially no methyl ester formation and no significant racemisation of the
product, the method of the present invention for production of a compound
of formula (II) or/and of lacosamide leads to an improved yield and an
improved enantiomeric purity of the product.
The invention also relates to important intermediates of the current process.
The most important intermediate (R)-2-N-Boc-amino-3-methoxypropanoic
acid (C-936) results from the improved O-methylation step according to the
present invention (see figure 1). The compound can be easily isolated from
the reaction mixture as the free acid or by forming a salt, such as e.g. a
cyclohexylammonium salt. Suitable C-936 salts are also regarded to be part
of the invention.
Also, (R)-N-benzyl-2-N-Boc-amino-3-methoxypropionamide (C-937) which
results from the benzyl amide formation (step 2 of figure 1) constitutes a part
of the invention.
Another aspect of the invention relates to the use of C-936 or C-937 or any
salt thereof as an adduct or intermediate in a method of producing (R)-2-
acetamido-N-benzyl-3-methoxypropionamide (lacosamide).

Yet another aspect of the invention pertains to a method of producing a
pharmaceutical formulation by the subsequent steps of
(a) producing lacosamide by the method according to the present
invention and
(b) mixing lacosamide with pharmaceutically acceptable excipients.
Another aspect of the present invention is a method of producing a
compound of formula VIII comprising the O-methylation of a compound of
formula VII,

to produce a compound of formula VIII,

wherein R4 is H, an N-protecting group, or/and a group having O-30 C atoms,
and
wherein R1 R2, and R3 are independently selected from H and groups having
O-30 C atoms,

characterized in that the O-methylation in being carried out in a one-step
reaction and wherein the compound of formula VIII is obtained in the same
configuration as the compound VII, of at least 88%, preferably of at least 90
%, more preferably of at least 95%, 96%, 97%, 98% or 99% enantiomeric
purity.
Preferably, R1, R2, and R3 are independently hydrogen, -OH, -SH, -NH2,
-NO2, -CN, -COOH, -SOH, -SO2H, -SO3H, halogen, -OR10, -SR10, -NR10R11,
-SOR10, -SO2R10, -SO3R10, substituted or unsubstituted alkyl as definied
above, substituted or unsubstituted C2-C6-alkenyl, substituted or
unsubstituted C2-C6-alkynyl, -(CO)-R10, -(CO)-O-R10, -O-(CO)-R10, substituted
or unsubstituted aryl as defined above, substituted or unsubstituted C3-C13-
hetaryl having 1-3 heteroatoms independently selected from N, S, O,
substituted or unsubstituted aralkyl as defined above, substituted or
unsubstituted C7-C15-alkaryl, substituted or unsubstituted C4-C14-hetaralkyl
having 1-3 heteroatoms independently selected from N, S, O; substituted or
unsubstituted C4-C14-alkhetaryl having 1-3 heteroatoms independently
selected from N, S, O; or substituted or unsubstituted C3-C12-cycloalkyl
having 0-3 heteroatoms independently selected from N, S, O.
It is more preferred that R1 is H, R2 is H or/and R3 is H. It is most preferred
that R1 is H, R2 is H and R3 is H.
Preferably, R4 is selected from R1 and N-protecting groups. More preferably,
R4 is the N-protecting group Rx as described above.
Even more preferred is R1 being H, R2 being H, R3 being H and R4 being Rx
as described above.
In the substituents R4, R1, R2, R3, the groups R10 and R11 are independently
hydrogen, substituted or unsubstituted alkyl as defined above, substituted or
unsubstituted C2-C6-alkenyl, substituted or unsubstituted C2-C6-aIkynyl,

substituted or unsubstituted aryl as defined above, substituted or
unsubstituted C3-C13-hetaryl having 1-3 heteroatoms independently selected
from N, S, O, substituted or unsubstituted aralkyl as defined above,
substituted or unsubstituted C2-C15-alkaryl, substituted or unsubstituted C4-
C14-hetaralkyl having 1-3 heteroatoms independently selected from N, S, O;
substituted or unsubstituted C4-C14-alkhetaryl having 1-3 heteroatoms
independently selected from N, S, O; or substituted or unsubstituted C3-C12-
cycloalkyl having O-3 heteroatoms independently selected from N, S, O.
Substitution in the groups R4, R1, R2, R3, R10 and R11 refers to substitution by
one or more substituents as defined above, e.g. by hydroxy, carboxy,
halogen, nitro, C1-C6 alkyl, C1-C6 alkoxy, amino, etc.
"The same configuration" of the compound VIII with reference to compound
VII means that essentially no racemisation takes place, or compound VIII is
obtained in the same configuration as compound VII with an enantiomeric
purity as defined above. If compound VII is in the R configuration, compound
VIII is also in the R configuration. If compound VII is in the S configuration,
compound VIII is also in the S configuration.
It is preferred that compound VII is in the R configuration.
The parameter of enantiomeric purity can be applied mutatis mutandis to
enantiomer mixtures. If compound VII is a mixture of the R and S
configuration, compound VIII is essentially the same mixture of the R and S
configuration, i.e. the ratio of the R and S configuration remains essentially
unaltered, or an enantiomeric ratio as follows is obtained. The obtained
enantiomeric ratio of compound VIII may be at least 88%, preferably at least
90 %, more preferably in at least 95, 96, 97, 98 or 99% of the enantiomeric
ratio of compound VII.
"One-step reaction" has the same meaning as discussed above.

The reaction schema of compound VII to compound VIII is a generalization
of the O-methylation of the present invention of the compound of formula I to
produce a compound of formula II as described above. If the compound VII
is in the R configuration, R1 is H, R2 is H, R3 is H and R4 is Rx, the compound
VIII corresponds to the compound of formula II and may be used for the
production of lacosamide, e.g. by the reaction steps as described above.
Starting from the compound II or VII, lacosamide may be produced by any
suitable method to introduce the N-benzylamide group and the N-acetyl
group. Therefore, in a particular preferred embodiment, the compound VII is
in the R configuration and R4 is Rx. It is most preferred that R4 is Rx, R1 is H,
R2 is H, and R3 is H and the compound VII is in the R configuration.
The inventive O-methylation of compound VII can be achieved by adding to
a compound of formula VII a methylation agent in the presence of an organo
metal compound, in particular an organo lithium compound, as defined
above. Suitable methylation agents are defined above. In an alternative
route, the selective O-methylation of the alcoholic group may be performed
by phase transfer catalysis as defined above.
Specific embodiments of the O-methylation of the compound of formula VII
correspond to the specific embodiments of the production method of
lacosamide comprising the O-methylation of the compound of formula I as
described above, in particular specific embodiments relating to the phase
transfer catalysis, phase transfer catalysts, in particular as defined in formula
IV, V, and VI, the phase transfer reaction system and its components, the
organo metal compound, reaction conditions during phase transfer catalysis
or in the presence of the organo metal compound, further reaction steps and
reaction conditions leading to lacosamide including N-benzylamide
formation, N-deprotection and N-acetylation, etc.
The yield of the methylation of compound VII by the method of the present
invention may be at least 85%, preferably at least 90% when using an
organo metal compound. When using PTC, the yield of methylation of

compound VII may be at least 85%, preferably at least 90%, even more
preferably at least 91%, 92%, 93%, 94%, 95 % or 96%.
Due to the fact that during O-methylation of D- or/and L-serine derivatives in
the method of the present invention, there is essentially no methyl ester
formation and no significant racemisation of the product, the method of the
present invention for production of a compound of formula VIII by
methylation of a compound of formula VII leads to an improved yield and an
improved enantiomeric purity of the product.
The invention is further illustrated by Figure 1 (comprising alternative steps
1 a or 1 b) and the following Examples:
Example 1: Production of (R)-2-N-Boc-amino-3-methoxypropanoic acid
(C-936) using butyllithium (step 1a)
A solution of N-Boc-D-serine (22g, 0.107 mol) in dry tetrahydrofuran (352ml)
was cooled to dry addition funnel 15% w/w n-butyllithium in hexanes (134ml, 0.216mol)
keeping the temperature 5'C. Dimethyl sulphate (12.1ml, 0.128mol) was added keeping the
temperature at O-5'C and the reaction mixture aged at O-5'C for 9 hours. The
reaction was quenched by the addition of water (110ml), basified to pH 10-13
with 30% sodium hydroxide (3ml) and the tetrahydrofuran/hexane evaporated
in vacuo. The residue was washed with toluene (44ml) and then acidified to a
pH of with methylene chloride (2x91ml, 1x66ml) and the combined C936 extracts
dried by azeotropic distillation. Yield on evaporation 23.7g, 100%. HPLC
purity 90.0%, Chiral purity 100%.
Example 2: Production of (R)-2-N-Boc-amino-3-methoxypropanoic acid
(C-936) using PTC (step 1b)

A suspension of N-Boc-D-serine (22g, 0.107 mol) and tetrabutylammonium
bromide (1.3g, 0.004mol) in toluene (110ml) was cooled to was added 20% sodium hydroxide (17.6ml, 0.107mol) keeping the
temperature (25.4ml, 0.485mol) were added keeping the temperature reaction mixture aged at 10"C for 1 hour. Water (66ml) was added to the
mixture and the phases separated. The aqueous layer was acidified to a pH of
and the combined C936 extracts dried by azeotropic distillation. (Yield on
evaporation 27.5g, 100%, HPLC purity 96.3%, Chiral purity 98.1%)
Example 3: Steps 2 to 4
(R)-N-benzyl-2-N-Boc-amino-3-methoxypropionamide (C-937) solution
(step 2)
The C936 solution prepared as above in example 2 was cooled to and isobutyl chloroformate (14.2ml, 0.107mol) at (11.8ml, 0.17mol) was added at added at hour the mixture was washed with water (44ml), 1N HCI (44ml), 8% sodium
bicarbonate (44ml) and water (44ml) to yield a C937 solution in methylene
chloride.
(R)-2-amino-N-benzyl-3-methoxypropionamide solution (step 3)
To the C937 solution prepared above was added 36% HCI (46.5ml,
0.541 mol) and the mixture aged for 1 hour. Water (66ml) was added and the
phases separated. The organic phase was extracted with water (22ml) and
the aqueous layers combined. The aqueous was basified to pH 1O-12 with
30% sodium hydroxide at aqueous layer was extracted with methylene chloride (2x110ml) and the
combined organic layers washed with water (44ml) yielding a methylene

chloride solution of (R)-2-amino-N-benzyl-3-methoxypropionamide.
Lacosamide (step 4)
The (R)-2-amino-N-benzyl-3-methoxypropionamide solution prepared above
was cooled to The reaction mixture is warmed to room temperature over 30 minutes and
aged for a further 30 minutes. The mixture is then washed with water (44ml),
8% sodium bicarbonate (44ml) and water (44ml). The methylene chloride was
exchanged for ethyl acetate by distillation and the solution distilled to a volume
of 115ml. The product was crystallised by cooling the solution to O-5'C and the
pure Lacosamide isolated by filtration (18.7g, 69.8%) HPLC purity 99.98%,
Chiral purity 99.8% ee.
Example 4: Isolation of (R)-2-N-Boc-amino-3-methoxypropanoic acid
(C-936)
The (R)-2-N-Boc-amino-3-rnethoxypropanoic acid (C-936) solution prepared
in example 1 was evaporated in vacuo yielding (R)-2-N-Boc-amino-3-
methoxypropanoic acid (C-936) as a waxy solid (23.7g, 100%). HPLC purity
90.0%. Elemental analysis Calculated for C9H17NO5 49.31% C; 7.82% H;
6.39% N. Found 49.12% C; 7.72% H; 8.97% N.
Example 5: Isolation of (R)-N-benzyl-2-N-Boc-amino-3-
methoxypropionamide (C-937)
The (R)-N-benzyl-2-N-Boc-amino-3-methoxypropionamide (C-937) solution
prepared in example 3 above was evaporated in vacuo yielding crude C937
as an oily solid. The crude solid (2g) was dissolved in 10% chloroform in
hexane (30ml) at 60'C, cooled to room temperature and left stand for 1 hour at
this temperature. The resulting solids were isolated by filtration yielding crude
C937 (1.1g). This crude solid was further recrystallised twice in 10 volumes of
10% chloroform in hexane to yield (R)-N-benzyl-2-N-Boc-amino-3-
methoxypropionamide (C-937) as a white crystalline solid (0.28g, 14%).

HPLC purity 97.3%. Elemental analysis Calculated for C16H24N2O5 62.32% C;
7.84% H; 9.08% N. Found 62.19% C; 7.79% H; 9.04% N.

WE CLAIM:
1. Method of producing (R)-2-acetamido-N-benzyl-3-
methoxypropionamide (lacosamide) comprising the O-methylation
of a compound of formula I

to produce a compound of formula II

wherein Rx is an N-protecting group,
characterised in that
the O-methylation is being carried out in a one-step reaction and
wherein the compound of formula II is obtained as an R-enantiomer of
at least 88 % purity and
the method is performed either (a) as a phase transfer catalysis or (b)
by adding a methylation agent and an organo lithium compound to
the compound of formula I.

2. Method as claimed in claim 1, wherein the method comprises the
addition of a methylation agent to a phase transfer reaction system
comprising the compound of formula I, an aqueous phase, an
organic phase and a phase transfer catalyst.
3. Method as claimed in claim 2, wherein a pyridinium, phosphonium,
ammonium or sulfonium salt is used as a phase transfer catalyst.
4. Method as claimed in anyone of claims 2-3, wherein the phase
transfer catalyst is chosen from compounds of (a) general formula
IV

(b) general formula V

or (c) general formula VI

wherein R, R', R" and R"', if present, are independently selectable
alkyl, aryl or aralkyl groups;
Q, in compounds of formula IV, is a nitrogen or phosphorus; and
X is a halide, acetate, p-toluenesulfonate, trifluoromethanesulfonate,
hexafluoroantimonate, hydroxide, perchlorate, hydrogensulfate,
thiocyanate or tetrafluoroborate group.
5. Method as claimed in anyone of claims 2-4, wherein the phase
transfer catalyst is tetrabutylammonium bromide.
6. Method as claimed in anyone of claims 2-5, wherein the
methylating agent used in the phase transfer catalysis is chosen from
dimethyl sulphate, trimethyl phosphate or methyl iodide.

7. Method as claimed in anyone of claims 2-6, wherein the aqueous
phase is aqueous sodium hydroxide, aqueous lithium hydroxide,
aqueous potassium hydroxide, aqueous sodium carbonate or aqueous
potassium carbonate.
8. Method as claimed in anyone of claims 2-7, wherein the organic
solvent is toluene, hexane, methylene chloride or methyl t-butyl ether.
9. Method as claimed in anyone of claims 1-8, wherein the phase
transfer catalysis is performed at O-10 °C for at least 30 minutes.
10. Method as claimed in claim 1, wherein the methylation agent used
with the organo lithium compound is dimethylsulfate.
11. Method as claimed in anyone of claims 1 or 10, wherein the
organo lithium compound is butyl lithium.
12. Method as claimed in claims 1 or 10 to 11, wherein the O-
methylation in the presence of an organo lithium compound takes
place at a temperature of O-10°C for at least 5 hrs.

13. Method as claimed in anyone of the preceding claims further
comprising the reaction of compound II with benzylamine to give the
compound of formula III,

and then replacing the protecting group Rx with methyl carbonyl to
give (R)-2-acetamido-N-benzyl-3-methoxypropionamide (lacosamide).
14. Method as claimed in claim 13, wherein the reaction of the
compound of formula II with benzylamine takes place in the presence
of an activator of the carboxyl group and a base.
15. Method as claimed in claim 14, wherein the base is 4-
methylmorpholine, triethylamine, diisopropylethylamine, 1.8-
diazabycyclo[5.4.0]undec-7-ene or potassium bicarbonate and the
activator of the carboxyl group is an alkyl chloroformate or a
carbodiimide.

16. Method as claimed in anyone of claims 13-15, wherein the N-
protecting group Rx is replaced by methyl carbonyl by successively
(a) cleaving off the protecting group Rx from the compound of formula
III by the addition of (i) a mineralic acid or (ii) H2/Pd-C to yield (R)-2-
amino-N-benzyl-3-methoxypropionamide and then (b) adding the
methyl carbonyl group to (R)-2-amino-N-benzyl-3-methoxyprop
ionamide by the reaction of (R)-2-amino-N-benzyl-3-
methoxypropionamide with acetic anhydride.
17. Method as claimed in claim 16, wherein step (b) is performed in
the absence of pyridine.
18. Method as claimed in anyone of claims 13-15 wherein the N-
protecting group Rx is cleaved off to obtain (R)-2-amino-N-benzyl-3-
methoxypropionamide.
19. Method as claimed in anyone of the preceding claims wherein
lacosamide is isolated from the final reaction mix by crystallisation.
20. Method as claimed in anyone of the preceding claims wherein the
N-protecting group is t-butyloxy carbonyl (Boc).

21. Method as claimed in any of the preceding claims comprising the
step of N-acetylation of (R)-2-amino-N-benzyl-3-methoxypropionamide
with acetic anhydride in the absence of a base, in particular in the
absence of pyridine.
22. Method as claimed in any one of the preceding claims wherein the
compound of formula II is (R)-2-N-Boc-amino-3-methoxypropanoic
acid (C-936) or salts thereof.

23. Method as claimed in any one of claims 13-18, wherein the
compound of formula III is (R)-N-benzyl-2-N-Boc-amino-3-
methoxypropionamide (C-937) or any salt thereof.
24. Method of producing a pharmaceutical formulation comprising
lacosamide by the subsequent steps of

(a) producing lacosamide by anyone of claims 1 to 22 and
(b) mixing lacosamide with pharmaceutical acceptable excipients.
25. Method as claimed in anyone of claims 1 to 22 further comprising
production of N-Boc-D-serine by reacting D-serine with di-t-butyl
dicarbonate in a phase transfer reaction.

26. Method of producing a compound of formula VIII comprising the
O-methylation of a compound of formula VII,

to produce a compound of formula VIII,

wherein R1, R2 and R3 are independently selected from hydrogen, -OH,
-SH, -NH2, -NO2, -CN, -COOH, -SOH, -SO2H, -SO3H, halogen, -OR10, -
SR10, -NR10R11, -SOR10, -SO2R10, -SO3R10, substituted or
unsubstituted alkyl, substituted or unsubstituted C2-C6-alkenyl,

substituted or unsubstituted C2-C6-alkynyl, -(CO)-R10, -(CO)-O-R10, -
O-(CO)-R10, substituted or unsubstituted aryl, substituted or
unsubstituted C3-C13-hetaryl having 1-3 heteroatoms independently
selected from N, S, O, substituted or unsubstituted aralkyl,
substituted or unsubstituted C7-C15-alkaryl, substituted or
unsubstituted C4-C14-hetaralkyl having 1-3 heteroatoms
independently selected from N, S, O; substituted or unsubstituted C4-
C14-alkhetaryl having 1-3 heteroatoms independently selected from N,
S, O; and substituted or unsubstituted C3-C12-cycloalkyl having O-3
heteroatoms independently selected from N, S, O,
wherein R4 is selected from R1 and N-protecting groups and wherein
R10 and R11 are independently selected from hydrogen, substituted or
unsubstituted alkyl, substituted or unsubstituted C2-C6-alkenyl,
substituted or unsubstituted C2-C6-alkynyl, substituted or
unsubstituted aryl, substituted or unsubstituted C3-C13-hetaryl
having 1-3 heteroatoms independently selected from N, S, O,
substituted or unsubstituted aralkyl, substituted or unsubstituted C7-
C15-alkaryl, substituted or unsubstituted C4-C14-hetaralkyl having 1-3
heteroatoms independently selected from N, S, O; substituted or
unsubstituted C4-C14-alkhetaryl having 1-3 heteroatoms

independently selected from N, S, O; and substituted or
unsubstituted C3-C12-cycloalkyl having O-3 heteroatoms
independently selected from N, S, O,
characterised in that the O-methylation is being carried out in a
one-step reaction wherein the compound of formula VIII is obtained in
the same configuration as the compound VII in at least 88 % purity
and the method is performed either (a) as a phase transfer catalysis or
(b) by adding a methylation agent and an organo lithium compound to
the compound of formula VII.
27. Method as claimed in claim 26, wherein R1 is H, R2 is H and R3 is
H and R4 is a N-protecting group.
28. Method as claimed in any of claims 26 or 27, wherein compound
VII is in the R-configuration.

ABSTRACT

TITLE: METHOD FOR PRODUCING LACOSAMIDE
The present invention relates to method of producing (R)-2-
acetamido-N-benzyl-3-methoxypropionamide (lacosamide)
comprising the O-methylation of a compound of formula I

to produce a compound of formula II

wherein Rx is an N-protecting group, characterised in that the O-
methylation is being carried out in a one-step reaction and wherein
the compound of formula II is obtained as an R-enantiomer of at least
88 % purity and the method is performed either (a) as a phase
transfer catalysis or (b) by adding a methylation agent and an organo
lithium compound to the compound of formula I.

METHOD FOR PRODUCING LACOSAMIDE

Documents:

Inventors:

PCT Conventions: