Title of Invention	"A PROCESS FOR THE PREPARATION OF NOVEL MACROPORUS BEADED CROSS LINKED CO-POLYMER"
Abstract	The present invention provides a process for the preparation of copolymer supports with an appropriate pore size distribution for affinity interactions of enzyme molecules with the ligands. The copolymer supports prepared by present process are rigid, stable over a wider range of pH and resistant to microbial attack, having longer operational life. The process comprises of polymerizing allyl glycidyl ether and a crosslinking monomer, wherein amount of allyl glycidyl ether and the crosslinking monomer used is in the range of 5 to 95% and from 95 to 5% respectively , in an inert atmosphere selected from nitrogen or argon, in the presence of a protective colloid, a porogen and a polymerization initiator.

Title of Invention

"A PROCESS FOR THE PREPARATION OF NOVEL MACROPORUS BEADED CROSS LINKED CO-POLYMER"

Abstract

The present invention provides a process for the preparation of copolymer supports with an appropriate pore size distribution for affinity interactions of enzyme molecules with the ligands. The copolymer supports prepared by present process are rigid, stable over a wider range of pH and resistant to microbial attack, having longer operational life. The process comprises of polymerizing allyl glycidyl ether and a crosslinking monomer, wherein amount of allyl glycidyl ether and the crosslinking monomer used is in the range of 5 to 95% and from 95 to 5% respectively , in an inert atmosphere selected from nitrogen or argon, in the presence of a protective colloid, a porogen and a polymerization initiator.

Full Text	This invention relates to a process for the preparation of novel macroporous beaded crosslinked copolymers. More particularly it relates to a process for the preparation of novel macroporous beaded crosslinked copolymers based on allyl glycidyl ether. The copolymers made by the process of this invention are useful as versatile copolymers for the preparation of affinity chromatography supports. The affinity chromatography supports are useful for the purification of industrial enzymes. Affinity chromatography supports are used in the isolation and purification of various enzymes. These materials are comprised of a rigid support of a specific particle size, pore size and its distribution. Further these supports provide a functional group on which a ligand can be linked. The ligands linked onto these supports display selective affinity interactions towards a particular enzyme. This recognition leads to specific binding of the enzyme of interest on to these supports through a ligand enzyme complex. The enzyme thus selectively bound is eluted by a subtle play of number of experimentally adjustable parameters. The requirements of an ideal support are : Insolubility, minimum non-specific adsorbtion hydrophilic/hydrophobic balance, mechanical strength, chemical stability, larger surface area, optimal particle size, resistance to microbial attack, resistance to variations in temperature and pH, a functional group for linking of the ligand etc. as described in 'Affinity chromatography: practical and theoretical aspects, Marcel Dekker, New York, 1985; Journal of Chromatography, Vol. 218, p 241, 1981 and Chemcial Technology, vol. 5, p 564, 1975. The different supports that are used in the preparation of affinity supports are: agarose, cellulose, crosslinked dextrans, porous glass, copolymers of silica, polyacrylamide and polystyrene as described in Methods in Enzymology, Vol. 34, 1974. These supports suffer from one or more of the following disadvantages : 1. The supports need activation by using toxic chemicals like cyanogen bromide during derivatization with ligands. 2. They are susceptible to microbial attack. 3. These supports lead to bed compression during operation. 4. The supports are not chemically stable over a wider range of pH. 5. The supports retain appreciable quantities of water thereby leading to larger elution volumes. The main object of the present invention is to provide a process for the preparation of novel macroporous beaded crosslinked beaded copolymer supports. Another object of the present invention is to provide a process for the preparation of copolymer supports with an active functional epoxy groups readily available for reaction with ligand molecules. Yet another object of the present invention is to provide a process for the preparation of copolymer supports with an appropriate pore size distribution for affinity interactions of enzyme molecules with the ligands. Still another object of the present invention is to provide a process for the preparation of copolymer supports, which are rigid, stable over a wider range of pH and resistant to microbial attack, so that longer operational life is achieved. Accordingly, the present invention provides a process for the preparation of novel macroporous beaded copolymers, which comprises of polymerizing allyl glycidyl ether and a crosslinking monomer such as herein described wherein amount of allyl glycidyl ether and the crosslinking monomer used is in the range of 5 to 95% and from 95 to 5% respectively , in an inert atmosphere selected from nitrogen or argon, in the presence of a protective colloid, a porogen and a polymerization initiator as defined herein , in an aqueous media, heating the polymerization reaction mixture at a temperature in the range of 60°C to 80°C for a period ranging from 2 to 4 hours, isolating the product by conventional methods, washing the resulting copolymer with water followed by a protic solvent as described herein and drying the resultant product at a temperature below 60°C to get the desired copolymer support. In an embodiment of the present invention, the crosslinking monomer used is selected from ethylene glycol dimethacrylate, divinyl benzene, pentaerythritol triacrylate, pentaerylthitol trimethacrylate, trimethylol propane trimethacrylate. In another embodiment of the invention, the protective colloid used may be such as poly vinyl pyrrolidone, poly vinyl acetate, sodium salt of poly acrylic acid, poly vinyl alcohol. In yet another embodiment of the invention, the inert gas used is chosen from nitrogen or argon. In yet another embodiment of the invention, the polymerization initiator used may be such as azobis isobutyronitrile, benzoyl peroxide or methyl ethyl ketone peroxide. In yet another embodiment of the invention, the protic solvent used for the washing of the copolymer matrices may be selected from alkanols such as methanol, ethanol. In still another embodiment of the invention, the protective colloid may be such as poly vinyl alcohol, salts of poly acrylic acid and poly methacrylic acid, poly vinyl pyrrolidone. In yet another embodiment of the invention, the porogen used may be such as decanol, octanol, butanol, cyclohexanol, preferably cyclohexanol. In a feature of the invention, the allyl glycidyl ether and the crosslinking monomer used may be in the range of 5 to 95 % and 95 to 5 % respectively. The process of the present invention is described with reference to the following Examples, which are illustrative only and should not be construed to limit the scope of this invention in any manner. Example 1 In an inert atmosphere of nitrogen, 1.5 grams of poly vinyl pyrrolidone was dissolved in 150 ml of distilled water in a 250 ml capacity glass reactor. 9.72 grams of allyl glycidyl ether, 8.38 grams of pentaerythritol triacrylate and 22.5 ml of cyclohexanol were added to the aqueous solution of poly vinyl pyrrolidone. The suspension was stirred at 300 rpm. 0.6 gram of azo bis isobutyronitrile was added to this suspension and the resultant polymerization reaction mixture was heated with agitation at a temperature of 70°C for 3 hours. The copolymer separated out as beads during the polymerization. The copolymer beads were isolated by filtration, washed with distilled water, followed by washing by methanol and dried in a vacuum oven at 40°C. The yield of copolymer obtained was 17.10 grams. Example 2 In an inert atmosphere of nitrogen, 1.5 grams of poly vinyl pyrrolidone was dissolved in 150 ml of distilled water in a 250 ml capacity glass reactor. 4.30 grams of allyl glycidyl ether, 14.50 grams of pentaerythritol trimethacrylate and 22.0 ml of cyclohexanol were added to the aqueous solution of poly vinyl pyrrolidone. The suspension was stirred at 300 rpm. 0.6 gram of azo bis isobutyronitrile was added to this suspension and the resultant polymerization reaction mixture was heated with agitation at a temperature of 70°C for 3 hours. The copolymer separated out as beads during the polymerization. The copolymer beads were isolated by filtration, washed with distilled water, followed by washing by methanol and dried in a vacuum oven at 40°C. The yield of copolymer obtained was 14.5 grams. Example 3 In an inert atmosphere of nitrogen, 2.0 grams of poly vinyl acetate was dissolved in 150 ml of distilled water in a 250 ml capacity glass reactor. 8.6 grams of allyl glycidyl ether, 7.8 grams of divinyl benzene and 23 ml of cyclohexanol were added to the aqueous solution of poly vinyl pyrrolidone. The suspension was stirred at 300 rpm. 0.8 gram of azo bis isobutyronitrile was added to this suspension and the resultant polymerization reaction mixture was heated with agitation at a temperature of 70oC for 3 hours. The copolymer separated out as beads during the polymerization. The copolymer beads were isolated by filtration, washed with distilled water, followed by washing by ethanol and dried in a vacuum oven at 50°C. The yield of copolymer obtained was 14.25 grams. Example 4 In an inert atmosphere of nitrogen, 1.8 grams of poly vinyl alcohol was dissolved in 150 ml of distilled water in a 250 ml capacity glass reactor. 9.0 grams of allyl glycidyl ether, 8 grams of ethylene glycol dimethacrylate and 43.5 ml of cyclohexanol were added to the aqueous solution of poly vinyl pyrrolidone. The suspension was stirred at 300 rpm. 0.6 gram of methyl ethyl ketone peroxide was added to this suspension and the resultant polymerization reaction mixture was heated with agitation at a temperature of 60°C for 4 hours. The copolymer separated out as beads during the polymerization. The copolymer beads were isolated by filtration, washed with distilled water, followed by washing by ethanol and dried in a vacuum oven at 40°C. The yield of copolymer obtained was 15.25 grams. Example 5 In an inert atmosphere of nitrogen, 1.5 grams of poly vinyl pyrrolidone was dissolved in 150 ml of distilled water in a 250 ml capacity glass reactor. 8.5 grams of allyl glycidyl ether, 8.25 grams of trimethylol propane triacrylate and 43.5 ml of cyclohexanol were added to the aqueous solution of poly vinyl pyrrolidone. The suspension was stirred at 300 rpm. 0.5 gram of benzoyl peroxide was added to this suspension and the resultant polymerization reaction mixture was heated with agitation at a temperature of 70°C for 3 hours. The copolymer separated out as beads during the polymerization. The copolymer beads were isolated by filtration, washed with distilled water and dried in a vacuum oven at 40°C. The yield of copolymer obtained was 15.35 grams. Example 6 In an inert atmosphere of nitrogen, 2.25 grams of sodium salt of poly acrylic acid was dissolved in 150 ml of distilled water in a 250 ml capacity glass reactor. 9.0 grams of allyl glycidyl ether, 8.75 grams of trimethylol propane trimethacrylate and 43.5 ml of cyclohexanol were added to the aqueous solution of poly vinyl pyrrolidone. The suspension was stirred at 300 rpm. 0.6 gram of benzoyl peroxide was added to this suspension and the resultant polymerization reaction mixture was heated with agitation at a temperature of 80°C for 2 hours. The copolymer separated out as beads during the polymerization. The copolymer beads were isolated by filtration, washed with distilled water, followed by washing by ethanol and dried in a vacuum oven at 50°C. The yield of copolymer obtained was 15.75 grams. Advantages of the invention The macroporous beaded crosslinked allyl glycidyl ether copolymer supports prepared as described herein have the following advantages : 1. The copolymer supports provide chemically active glycidyl groups for derivatization with ligands. 2. The copolymer supports have appropriate pore size and pore size distribution for free translocation of enzyme molecules. 3. The copolymer supports are chemically stable over a wider range of pH from 2 to 11. 4. The copolymer supports are rigid. 5. The copolymer supports do not require activation or derivatisation for linking of ligand. 6. The copolymer supports are resistant to microbial degradation. 7. The copolymer supports do not retain water. We Claim: 1. A process for the preparation of novel macroporous beaded copolymers, which comprises of polymerizing allyl glycidyl ether and a crosslinking monomer such as herein described wherein amount of allyl glycidyl ether and the crosslinking monomer used is in the range of 5 to 95% and from 95 to 5% respectively , in an inert atmosphere selected from nitrogen or argon, in the presence of a protective colloid, a porogen and a polymerization initiator as defined herein , in an aqueous media, heating the polymerization reaction mixture at a temperature in the range of 60°C to 80°C for a period ranging from 2 to 4 hours, isolating the product by conventional methods, washing the resulting copolymer with water followed by a protic solvent as described herein and drying the resultant product at a temperature below 60°C to get the desired copolymer support. 2. A process as claimed in claim 1, wherein the crosslinking monomer used is selected from ethylene glycol dimethacrylate, divinyl benzene, pentaerythritol triacrylate, pentaerythritol trimethacrylate, trimethylol propane trimethacrylate, trimethylol propane triacrylate. 3. A process as claimed in claims 1 - 2 wherein, the protective colloid used is selected from poly vinyl pyrrolidone, poly vinyl acetate, poly vinyl alcohol, sodium salt of poly acrylic acid. 4. A process as claimed in claims 1 to 4 wherein, the polymerization initiator used is suchajs azobis isobutyronitrile, benzoyl peroxide or methyl ethyl ketone peroxide. 5. A process as claimed in claims 1 to 4, wherein the protic solvent used is selected from water, alkanols such as methanol, ethanol. 6. A process as claimed in claims 1 - 6 wherein, the porogen used is alcohols selected from decanol, octanol, butnol, cyclohexanol. A process for the preparation of novel macroporous beaded crossiinked copolymer supports as herein described with reference to the Examples 1 to 6.

Full Text

This invention relates to a process for the preparation of novel macroporous beaded crosslinked copolymers. More particularly it relates to a process for the preparation of novel macroporous beaded crosslinked copolymers based on allyl glycidyl ether. The copolymers made by the process of this invention are useful as versatile copolymers for the preparation of affinity chromatography supports. The affinity chromatography supports are useful for the purification of industrial enzymes.
Affinity chromatography supports are used in the isolation and purification of various enzymes. These materials are comprised of a rigid support of a specific particle size, pore size and its distribution. Further these supports provide a functional group on which a ligand can be linked. The ligands linked onto these supports display selective affinity interactions towards a particular enzyme. This recognition leads to specific binding of the enzyme of interest on to these supports through a ligand enzyme complex. The enzyme thus selectively bound is eluted by a subtle play of number of experimentally adjustable parameters.
The requirements of an ideal support are :
Insolubility, minimum non-specific adsorbtion hydrophilic/hydrophobic balance, mechanical strength, chemical stability, larger surface area, optimal particle size, resistance to microbial attack, resistance to variations in temperature and pH, a functional group for linking of the ligand etc. as described in 'Affinity chromatography: practical and theoretical aspects, Marcel Dekker, New York, 1985; Journal of Chromatography, Vol. 218, p 241, 1981 and Chemcial Technology, vol. 5, p 564, 1975.
The different supports that are used in the preparation of affinity supports are: agarose, cellulose, crosslinked dextrans, porous glass, copolymers of silica, polyacrylamide and polystyrene as described in Methods in Enzymology, Vol. 34, 1974.
These supports suffer from one or more of the following disadvantages :
1. The supports need activation by using toxic chemicals like cyanogen bromide during derivatization with ligands.
2. They are susceptible to microbial attack.
3. These supports lead to bed compression during operation.
4. The supports are not chemically stable over a wider range of pH.
5. The supports retain appreciable quantities of water thereby leading to larger elution volumes.
The main object of the present invention is to provide a process for the preparation of novel macroporous beaded crosslinked beaded copolymer supports.
Another object of the present invention is to provide a process for the preparation of copolymer supports with an active functional epoxy groups readily available for reaction with ligand molecules.
Yet another object of the present invention is to provide a process for the preparation of copolymer supports with an appropriate pore size distribution for affinity interactions of enzyme molecules with the ligands. Still another object of the present invention is to provide a process for the preparation of copolymer supports, which are rigid, stable over a wider range of pH and resistant to microbial attack, so that longer operational life is achieved.
Accordingly, the present invention provides a process for the preparation of novel macroporous beaded copolymers, which comprises of polymerizing allyl glycidyl ether and a crosslinking monomer such as herein described wherein amount of allyl glycidyl ether and the crosslinking monomer used is in the range of 5 to 95% and from 95 to 5% respectively , in an inert atmosphere selected from nitrogen or argon, in the presence of a protective colloid, a porogen and a polymerization initiator as defined herein , in an aqueous media, heating the polymerization reaction mixture at a temperature in the range of 60°C to 80°C for a period ranging from 2 to 4 hours, isolating the product by conventional methods, washing the resulting copolymer with water followed by a protic solvent as described herein and drying the resultant product at a temperature below 60°C to get the desired copolymer support.
In an embodiment of the present invention, the crosslinking monomer used is selected from ethylene glycol dimethacrylate, divinyl benzene, pentaerythritol triacrylate, pentaerylthitol trimethacrylate, trimethylol propane trimethacrylate.

In another embodiment of the invention, the protective colloid used may be such as poly vinyl pyrrolidone, poly vinyl acetate, sodium salt of poly acrylic acid, poly vinyl alcohol.
In yet another embodiment of the invention, the inert gas used is chosen from nitrogen or argon.
In yet another embodiment of the invention, the polymerization initiator used may be such as azobis isobutyronitrile, benzoyl peroxide or methyl ethyl ketone peroxide.
In yet another embodiment of the invention, the protic solvent used for the washing of the copolymer matrices may be selected from alkanols such as methanol, ethanol.
In still another embodiment of the invention, the protective colloid may be such as poly vinyl alcohol, salts of poly acrylic acid and poly methacrylic acid, poly vinyl pyrrolidone.
In yet another embodiment of the invention, the porogen used may be such as decanol, octanol, butanol, cyclohexanol, preferably cyclohexanol.
In a feature of the invention, the allyl glycidyl ether and the crosslinking monomer used may be in the range of 5 to 95 % and 95 to 5 % respectively.
The process of the present invention is described with reference to the following Examples, which are illustrative only and should not be construed to limit the scope of this invention in any manner.
Example 1
In an inert atmosphere of nitrogen, 1.5 grams of poly vinyl pyrrolidone was dissolved in 150 ml of distilled water in a 250 ml capacity glass reactor. 9.72 grams of allyl glycidyl ether, 8.38 grams of pentaerythritol triacrylate and 22.5 ml of cyclohexanol were added to the aqueous solution of poly vinyl pyrrolidone. The suspension was stirred at 300 rpm. 0.6 gram of azo bis isobutyronitrile was added to this suspension and the resultant polymerization reaction mixture was heated with agitation at a temperature of 70°C for 3 hours. The copolymer separated out as beads during the polymerization. The copolymer beads were isolated by filtration, washed with distilled water, followed by washing by methanol and dried in a vacuum oven at 40°C. The yield of copolymer obtained was 17.10 grams.
Example 2
In an inert atmosphere of nitrogen, 1.5 grams of poly vinyl pyrrolidone was dissolved in 150 ml of distilled water in a 250 ml capacity glass reactor. 4.30 grams of allyl glycidyl ether, 14.50 grams of pentaerythritol trimethacrylate and 22.0 ml of cyclohexanol were added to the aqueous solution of poly vinyl pyrrolidone. The suspension was stirred at 300 rpm. 0.6 gram of azo bis isobutyronitrile was added to this suspension and the resultant polymerization reaction mixture was heated with
agitation at a temperature of 70°C for 3 hours. The copolymer separated out as beads during the polymerization. The copolymer beads were isolated by filtration, washed with distilled water, followed by washing by methanol and dried in a vacuum oven at 40°C. The yield of copolymer obtained was 14.5 grams.
Example 3 In an inert atmosphere of nitrogen, 2.0 grams of poly vinyl acetate was dissolved in 150 ml of distilled water in a 250 ml capacity glass reactor. 8.6 grams of allyl glycidyl ether, 7.8 grams of divinyl benzene and 23 ml of cyclohexanol were added to the aqueous solution of poly vinyl pyrrolidone. The suspension was stirred at 300 rpm. 0.8 gram of azo bis isobutyronitrile was added to this suspension and the resultant polymerization reaction mixture was heated with agitation at a temperature of 70oC for 3 hours. The copolymer separated out as beads during the polymerization. The copolymer beads were isolated by filtration, washed with distilled water, followed by washing by ethanol and dried in a vacuum oven at 50°C. The yield of copolymer obtained was 14.25 grams.
Example 4
In an inert atmosphere of nitrogen, 1.8 grams of poly vinyl alcohol was dissolved in 150 ml of distilled water in a 250 ml capacity glass reactor. 9.0 grams of allyl glycidyl ether, 8 grams of ethylene glycol dimethacrylate and 43.5 ml of cyclohexanol were added to the aqueous solution of poly vinyl pyrrolidone. The suspension was stirred at 300 rpm. 0.6 gram of methyl ethyl ketone peroxide was added to this suspension and the resultant polymerization reaction mixture was heated with agitation at a temperature of 60°C for 4
hours. The copolymer separated out as beads during the polymerization. The copolymer beads were isolated by filtration, washed with distilled water, followed by washing by ethanol and dried in a vacuum oven at 40°C. The yield of copolymer obtained was 15.25 grams.
Example 5
In an inert atmosphere of nitrogen, 1.5 grams of poly vinyl pyrrolidone was dissolved in 150 ml of distilled water in a 250 ml capacity glass reactor. 8.5 grams of allyl glycidyl ether, 8.25 grams of trimethylol propane triacrylate and 43.5 ml of cyclohexanol were added to the aqueous solution of poly vinyl pyrrolidone. The suspension was stirred at 300 rpm. 0.5 gram of benzoyl peroxide was added to this suspension and the resultant polymerization reaction mixture was heated with agitation at a temperature of 70°C for 3 hours. The copolymer separated out as beads during the polymerization. The copolymer beads were isolated by filtration, washed with distilled water and dried in a vacuum oven at 40°C. The yield of copolymer obtained was 15.35 grams.
Example 6
In an inert atmosphere of nitrogen, 2.25 grams of sodium salt of poly acrylic acid was dissolved in 150 ml of distilled water in a 250 ml capacity glass reactor. 9.0 grams of allyl glycidyl ether, 8.75 grams of trimethylol propane trimethacrylate and 43.5 ml of cyclohexanol were added to the aqueous solution of poly vinyl pyrrolidone. The suspension was stirred at 300 rpm. 0.6 gram of benzoyl peroxide was added to this suspension and the resultant polymerization reaction mixture was heated with
agitation at a temperature of 80°C for 2 hours. The copolymer separated out as beads during the polymerization. The copolymer beads were isolated by filtration, washed with distilled water, followed by washing by ethanol and dried in a vacuum oven at 50°C. The yield of copolymer obtained was 15.75 grams.
Advantages of the invention
The macroporous beaded crosslinked allyl glycidyl ether copolymer supports prepared
as described herein have the following advantages :
1. The copolymer supports provide chemically active glycidyl groups for derivatization with ligands.
2. The copolymer supports have appropriate pore size and pore size distribution for free translocation of enzyme molecules.
3. The copolymer supports are chemically stable over a wider range of pH from 2 to 11.
4. The copolymer supports are rigid.
5. The copolymer supports do not require activation or derivatisation for linking of ligand.
6. The copolymer supports are resistant to microbial degradation.
7. The copolymer supports do not retain water.

We Claim:
1. A process for the preparation of novel macroporous beaded copolymers, which comprises of polymerizing allyl glycidyl ether and a crosslinking monomer such as herein described wherein amount of allyl glycidyl ether and the crosslinking monomer used is in the range of 5 to 95% and from 95 to 5% respectively , in an inert atmosphere selected from nitrogen or argon, in the presence of a protective colloid, a porogen and a polymerization initiator as defined herein , in an aqueous media, heating the polymerization reaction mixture at a temperature in the range of 60°C to 80°C for a period ranging from 2 to 4 hours, isolating the product by conventional methods, washing the resulting copolymer with water followed by a protic solvent as described herein and drying the resultant product at a temperature below 60°C to get the desired copolymer support.
2. A process as claimed in claim 1, wherein the crosslinking monomer used is selected from ethylene glycol dimethacrylate, divinyl benzene, pentaerythritol triacrylate, pentaerythritol trimethacrylate, trimethylol propane trimethacrylate, trimethylol propane triacrylate.
3. A process as claimed in claims 1 - 2 wherein, the protective colloid used is selected from poly vinyl pyrrolidone, poly vinyl acetate, poly vinyl alcohol, sodium salt of poly acrylic acid.
4. A process as claimed in claims 1 to 4 wherein, the polymerization initiator used is suchajs azobis isobutyronitrile, benzoyl peroxide or methyl ethyl ketone peroxide.
5. A process as claimed in claims 1 to 4, wherein the protic solvent used is selected from water, alkanols such as methanol, ethanol.
6. A process as claimed in claims 1 - 6 wherein, the porogen used is alcohols selected from decanol, octanol, butnol, cyclohexanol.
A process for the preparation of novel macroporous beaded crossiinked copolymer supports as herein described with reference to the Examples 1 to 6.

Documents:

1059-del-2000-abstract.pdf

1059-del-2000-claims.pdf

1059-del-2000-correspondence-others.pdf

1059-del-2000-correspondence-po.pdf

1059-del-2000-description (complete).pdf

1059-del-2000-form-1.pdf

1059-del-2000-form-19.pdf

1059-del-2000-form-2.pdf

1059-del-2000-form-3.pdf

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Patent Number

254221

Indian Patent Application Number

1059/DEL/2000

PG Journal Number

41/2012

Publication Date

12-Oct-2012

Grant Date

04-Oct-2012

Date of Filing

24-Nov-2000

Name of Patentee

COUNCIL OF SCIENTIFIC AND INDUSTRIAL RESEARCH

Applicant Address

RAFI MARG, NEW DELHI - 110 001, INDIA.

Inventors:

#	Inventor's Name	Inventor's Address
1	VARSHA BHIKOBA GHADGE	NATIONAL CHEMICAL LABORATORY, PUNE 411008, MAHARASHTRA, INDIA.
2	CHELANATH KHIZHAKKE MADATH RANJAN	NATIONAL CHEMICAL LABORATORY, PUNE 411008, MAHARASHTRA, INDIA.
3	MADATH RANJAN	NATIONAL CHEMICAL LABORATORY, PUNE 411008, MAHARASHTRA, INDIA.
4	SURENDRA PONARATHNAM	NATIONAL CHEMICAL LABORATORY, PUNE 411008, MAHARASHTRA, INDIA.

PCT International Classification Number

C08F 016/00

PCT International Application Number

N/A

PCT International Filing date

PCT Conventions:

#	PCT Application Number	Date of Convention	Priority Country
1			NA