Title of Invention	AN INJECTOR DEVICE
Abstract	The invention relates to a method and device for treating or preventing or suppressing a disease or condition in a non-human animal. The method comprises the steps of providing a single delivery device containing two components for sequential delivery from the delivery device. A first component is delivered from the single delivery device into a teat canal of a non-human animal and subsequently the second component is delivered from the single delivery device into the teat canal. The components are delivered without substantial mixing of the components.

Title of Invention

AN INJECTOR DEVICE

Abstract

The invention relates to a method and device for treating or preventing or suppressing a disease or condition in a non-human animal. The method comprises the steps of providing a single delivery device containing two components for sequential delivery from the delivery device. A first component is delivered from the single delivery device into a teat canal of a non-human animal and subsequently the second component is delivered from the single delivery device into the teat canal. The components are delivered without substantial mixing of the components.

Full Text	1 METHOD AND DEVICE FOR TREATING A TEAT CANAL OF AN ANIMAL Introduction The invention relates to a method and device for administering two components into the teat canal of a non-human animal. Bovine mastitis is a severe, potentially fatal, inflammatory disease of the udder, caused by a broad range of infectious organisms, but most notably by various Gram positive bacteria of the genera Staphylococcus and Streptococcus and the Gram negative species, Escherichia coli. The infection usually enters the udder via the teat or streak canal. Mastitis is treated by a variety of antibiotic cerates, infused into the udder via the teat or streak canal. In severe cases, high doses of antibiotic are also given by injection. A high proportion of mastitic infections are contracted during the "dry" period, which precedes calving. The infection may later become clinically significant either during the dry period, or after calving when lactation has resumed. It is known to treat mastitis using a twin injector pack, one injector containing an anti-bacterial formulation and a second injector containing a seal or barrier formulation. The anti-bacterial formulation is delivered first to the teat canal followed by delivery of the seal formulation forming a physical barrier in the teat canal against the entry of bacteria into the udder. These twin injector packs are sold under the name Teat Seal™. WO94/13261 and WO95/31180 describe the twin injectors in detail and are hereby incorporated by reference. While the twin injector system provides an effective method to reduce the incidence of clinical mastitis administration of the injectors can be time consuming, doubles the risk of introducing extraneous environmental bacteria and doubles the risk of causing damage to the epithelium of the streak canal. The use of two injectors also increases the cost of treatment and creates additional non-degradable waste. WO 2005/072644 PCT/IE2005/000007 2 There is a need for an improved method and device for preventing intrammary disorders which will overcome at least some of these problems. Statements of Invention According to another aspect of the invention there is provided use of a single delivery device for treating or preventing or suppressing a disease or condition in a non-human animal comprising the steps of: - providing a single delivery device containing two components for sequential delivery from the delivery device; delivering a first component from the single delivery device into a teat canal of anon-human animal; and subsequently delivering the second component from the single delivery device into the teat canal without substantial mixing of the components. In one embodiment the delivery device comprises an injector device containing the two components, the components being separated by a barrier and the method comprises the steps of: - delivering the first component from the injector device; at least partially releasing the barrier; and subsequently delivering the second component from the injector device. WO 2005/072644 PCT/IE2005/000007 3 In another embodiment the disease or condition is mastitis and the use is for treating or preventing a mastitis-causing micro-organism. In one embodiment the second component comprises a seal. According to another aspect of the invention there is provided use of a single injector device for treating preventing or suppressing mastitis or a mastitis causing microorganism comprising the steps of sequentially delivering from a single delivery device an antimicrobial formulation and a seal formulation into the teat canal of a non-human animal wherein the antimicrobial formulation and the seal formulation are delivered into the teat canal without substantial mixing of the formulations prior to delivery into the teat canal. In one embodiment the seal formulation comprises a non-toxic heavy metal salt. In another embodiment the seal formulation comprises greater than 40% by weight of the heavy metal salt. In a further embodiment the seal formulation comprises between 50% and 75% by weight of the heavy metal salt. In one embodiment the seal formulation comprises approximately 65% by weight of the heavy metal salt. In another embodiment the heavy metal is bismuth. In a further embodiment the salt is a sub-nitrate salt. In one embodiment the seal formulation comprises a gel base. In another embodiment the gel base is a gel based on aluminium stearate. WO 2005/072644 PCT/IE2005/000007 4 In a further embodiment the gel base includes liquid paraffin as a vehicle. In one embodiment the first component comprises an antimicrobial. In another embodiment the antimicrobial is selected from any one or more of betalactam antibiotics, polymyxins, glycopeptides, aminoglycosides, lincosamides, macrolides, pleuromutilins, "fenicols" such as chloramphenicol and florfenicol, tetracylcines, sulphonamides and potentiated sulphonamides such as mixtures of trimethoprim and one or more sulphonamide, quinolones and fluoroquinolones, ionophores, courmarins such as novobiocin, natural or synthetic peptides, aminoglycosides, antimicrobial peptides or antimicrobials, lantibiotics, or other products of bacteria and other micro-organisms. In a further embodiment the betalactam is selected from any one or more of penicillin, modified penicillin such as cloxacillin, amoxycillin, ampicillin, cephalosporins or beta lactam antibiotics potentiated by beta lactamase inhibitors such as clavulanic acid. In one embodiment the aminoglycoside is selected from any one or more of streptomycin, dihydrostreptomycin, neomycin, gentamycin, framycetin, aparamycin or kanamycin. In another embodiment the antimicrobial is selected from any one or more of macrolide, lincosamide or pleuromutilin, erythromycin, spiramycin, tylosin, spiramycin, tilmicosin, lincomycin, spectinomysin, pirlimycin or tiamulin. In a further embodiment the antimicrobial is selected from any one or more of potentiated sulphonamide mixtures, trimethoprim plus sulphadiazine, sulphadimidine, sulphadoxine, sulphadimethoxine or other sulphonamide, WO 2005/072644 PCT/IE2005/000007 5 oxytetracycline, minocycline or doxyclycline, fluoroquinolones, enrofloxacin, ciprofloxacin, norfloxacin, danofloxacin, difloxacin or niarbofloxacin. In one embodiment the first component comprises an anti-inflammatory. In another embodiment the anti-inflammatory is selected from any one or more of steroids such as prednisolone, betamethazone, dexamethazone, phenylbutazone, or non-steroids such as fiunixin, ketoprofen, carprofen, vedaprofen, meloxicam, tepoxalin, eltenac, nimesulide or tolfenamic acid. According to further aspect of the invention there is provided an injector device for delivery of components into the teat canal of a non-human animal comprising: a barrel for containing a first component, an outlet nozzle at one end of the barrel, an internal receptacle for containing a second component, a barrier for separating a first component and a second component, and a delivery means for delivery of a first component from the barrel and sequential delivery of a second component from the internal receptacle through the outlet nozzle. In one embodiment the barrier is normally closed. In another embodiment the barrier is releasable for delivery of the second component. WO 2005/072644 PCT/IE2005/000007 6 In a further embodiment the barrier is defined by at least a portion of the internal receptacle. In one embodiment the barrier comprises one or more passageways. In another embodiment the one or more passageways are opened when the barrier is released for delivery of the second component through said one or more passageways. In a further embodiment the device comprises an activator for releasing the barrier. In one embodiment the activator comprises a mechanical activator means. In another embodiment the activator comprises at least one projecting member. In a further embodiment the activator is located in the barrel. In one embodiment the activator is located adjacent to the outlet nozzle. In another embodiment the activator comprises one or more passageways. In a further embodiment the activator is configured for engagement with the internal receptacle to provide a direct passageway for delivery of the second component from the internal receptacle into the outlet nozzle. In one embodiment the delivery means comprises a plunger for the barrel, In another embodiment the barrier is released by the delivery means. In a further embodiment the internal receptacle comprises an inner barrel located within an outer barrel defined by the barrel of the injector. WO 2005/072644 PCT/IE2005/000007 7 In one embodiment the inner barrel is a close fit within the outer barrel. In another embodiment the delivery means comprises the inner barrel. In a further embodiment the inner barrel defines a plunger for the outer barrel. In one embodiment the delivery means comprises a plunger for the inner barrel. In another embodiment the inner barrel comprises engagement means for engagement with the outer barrel on assembly. In a further embodiment the engagement means comprises an external seal. In one embodiment the outer barrel comprises engagement means for engagement with the inner barrel. In another embodiment the outer barrel comprises a locking ring for engagement with the inner barrel. In a further embodiment the inner barrel comprises engagement means for engagement with the plunger. In one embodiment the inner barrel comprises a locking ring for engagement with the plunger. In another embodiment the receptacle comprises a bag. In a further embodiment the receptacle comprises a capsule. WO 2005/072644 PCT/IE2005/000007 8 In one embodiment the receptacle is attached to or forms an integral part of the delivery means. In another embodiment the activator comprises a rupturing means for at least partially releasing the barrier. In a further embodiment the rupturing means comprises a mechanical rupture member. In one embodiment the rupture member comprises at least one blade. In another embodiment the rupture member comprises at least one tooth. In a further embodiment the rupture member is located in the barrel. In one embodiment the rupture member is located adjacent to the outlet nozzle. In another embodiment the barrel contains a first component. In a further embodiment the first component comprises an antimicrobial formulation. In one embodiment the internal receptacle contains a second component. In another embodiment the second component comprises a seal formulation. In a further embodiment a first component is delivered from the barrel and a second component is subsequently delivered from the internal receptacle without substantial mixing of the components. WO 2005/072644 PCT/IE2005/000007 9 In one embodiment the seal formulation may comprise the seal formulation as described above. In another embodiment the antimicrobial formulation may comprise the antimicrobial formulation as described above. According to the invention there is provided a method for treating or preventing or suppressing a disease or condition in a non-human animal comprising the steps of: - providing a single delivery device containing two components for sequential delivery from the delivery device; delivering a first component from the single delivery device into a teat canal of a non-human animal; and subsequently delivering the second component from the single delivery device into the teat canal without substantial mixing of the components. In one embodiment the delivery device comprises an injector device containing the two components, the components being separated by a barrier and the method comprises the steps of: - delivering the first component from the injector device; at least partially releasing the barrier; and subsequently delivering the second component from the injector device. WO 2005/072644 PCT/IE2005/000007 10 In another embodiment the disease or condition is mastitis and the method of the invention is for treating or preventing a mastitis-causing micro-organism. In a further embodiment the second component comprises a seal. According to a further aspect the invention provides a method for treating, preventing or suppressing mastitis or a mastitis causing micro-organism comprising the steps of sequentially delivering, from a single delivery device an antimicrobial formulation and a seal formulation into the teat canal of a non-human animal wherein the antimicrobial formulation and the seal formulation are delivered into the teat canal without substantial mixing of the formulations prior to delivery into the teat canal. In one embodiment the seal formulation comprises a non-toxic heavy metal salt. ii In another embodiment the seal formulation comprises greater than 40% by weight of the heavy metal salt. In a further embodiment the seal formulation comprises between 50% and 75% by weight of the heavy metal salt. In one embodiment the seal formulation comprises approximately 65% by weight of the heavy metal salt. In another embodiment the heavy metal is bismuth. In a further embodiment the salt is a sub-nitrate salt. In one embodiment the seal formulation comprises a gel base. In another embodiment the gel base is a gel based on aluminium stearate. WO 2005/072644 PCT/IE2005/000007 11 In a further embodiment the gel base includes liquid paraffin as a vehicle. In one embodiment the first component comprises an antimicrobial. In another embodiment the antimicrobial is selected from any one or more of betalactam antibiotics, polymyxins, glycopeptides, aminoglycosides, lincosamides, macrolides, pleiiromutilins, "fenicols" such as chloramphenicol and florfenicol., tetracylcines, sulphonamides and potentiated sulphonamides such as mixtures of trimethoprim and one or more sulphonamide, quinolones and fluoroquinolones, ionophores, courmarins such as novobiocin, natural or synthetic peptides, aminoglycosides, antimicrobial peptides or antimicrobials, lantibiotics, or other products of bacteria and other micro-organisms. In a further embodiment the betalactam is selected from any one or more of penicillin, modified penicillin such as cloxacillin, amoxycillin, ampicillin, cephalosporins or beta lactam antibiotics potentiated by beta lactamase inhibitors such as clavulanic acid. In one embodiment the aminoglycoside is selected from any one or more of streptomycin, dihydrostreptomycin, neomycin, gentamycin, framycetin, aparamycin or kanamycin. In another embodiment the antimicrobial is selected from any one or more of rnacrolide, lincosamide or pleuromutilin, erythromycin, spiramycin, tylosin, spiramycin, tilmicosin, lincomycin, spectinomysin, pirlimycin or tiamulin. In a further embodiment the antimicrobial is selected from any one or more of potentiated sulphonamide mixtures, trimethoprim plus sulphadiazine, sulphadimidine, sulphadoxine, sulphadimethoxine or other sulphonamide, WO 2005/072644 PCT/IE2005/000007 12 oxytetracycline, minocycline or doxyclycline, fluoroquinolones, enrofloxacin, ciprofloxacin, nprfloxacin, danofloxacin, difloxacin or marbofloxacin. In one embodiment the first component comprises an anti-inflammatory. In another embodiment the anti-inflammatory is selected from any one or more of steroids such as prednisolone, betamethazone, dexamethazone, phenylbutazone, or non-steroids such as flunixin, ketoprofen, carprofen, vedaprofen, meloxicam, tepoxalin, eltenac, nimesulide or tolfenamic acid. Brief description of Drawings The invention will be more clearly understood from the following description thereof given by way of example only with reference to the accompanying drawings, in which: - Fig. 1 is a schematic cross sectional view of an injector device according to the invention; Figs. 2 is a cross sectional view of an internal barrel of the device; Fig. 3 is a cross sectional view of the internal barrel of Fig. 2 with a seal component in place; Figs. 4 is a cross sectional view of the internal barrel of Fig. 3 with a plunger inserted; Fig. 5 is a cross sectional view of an external barrel of the device; WO 2005/072644 PCT/IE2005/000007 13 Fig. 6 is a cross sectional view of the external barrel of Fig. 5 with an antimicrobial component in place; Fig. 7 is a cross sectional view of the assembled injector device; Figs. 8(a) to 8(c) are cross sectional views of the injector device, in use; Fig. 9 is a cross sectional view of another injector of the invention; Fig. 9(a) is an enlarged view of portion of the injector in Fig. 9; Figs. 10 to 12 are cross sectional views of a further injector device according to the invention in different configurations of use; Fig. 13 is a top plan view of the device of Fig. 9; Figs. 14 (a) to 14 (c) are cross sectional views of a further injector device according to the invention in different configurations of use; Fig 15 is an exploded broken out view of components of the injector of Fig.14; Figs. 16 (a) is a detailed cross sectional view of a portion of the injector of Fig. 14, and Figs. 16(b) and 16 (c) are detailed perspective views of a portion of the device in the configurations of Fig. 14 (b) and (c) respectively; Fig. 17 is a schematic cross sectional view of an injector device according to a further embodiment of the invention; Figs. 18(a) to 18(d) are cross sectional views of the device of Fig. 17, in use; 14 Fig. 19 is a schematic cross sectional view of another injector device according to the invention; and Figs. 20(a) to 20(c) are cross sectional views of the injector device of Fig. 19, in use. Detailed Description The invention provides an injector device that allows for the sequential delivery of two incompatible components such as an antimicrobial formulation and a seal formulation into the teat canal of a non-human animal. The seal formulation and antimicrobial formulation are contained separately within a single injector device. This enables the product to be stored without affecting the stability of either component. The device also provides for the delivery of the antimicrobial formulation ahead of the seal formulation which effectively forms a physical barrier in the teat canal preventing any further entry into the teat canal. The seal formulation also prevents the possibility of the antimicrobial phase leaking or being expressed from the teat by gravitational or hydrostatic forces. The seal formulation may comprise a viscous oil-based cerate containing a high proportion of a heavy metal salt, bismuth subnitrate. The product Teat Seal (trade mark of Cross Vetpharm Group) is described in detail in WO98/26759 and comprises a non-toxic heavy metal salt in a gel base. The base is a gel based on aluminium stearate. The gel preferably includes a vehicle such as liquid paraffin. The gel may also comprise a polyethylene gel. The gel may be based on low density polyethylene or on high density polyethylene. Preferably, the heavy metal salt is present in an amount of greater than 40%, preferably between 50% and 75% by weight, most preferably approximately 65% by weight. The seal formulation prevents infection entering the udder via the teat or streak canal through a combination of its viscosity, density and adhesiveness. WO 2005/072644 PCT/IE2005/000007 15 The antimicrobial or anti-inflammatory formulation may be selected from any one or more of a wide variety of compounds that are known to be effective for the treatment, prevention and elimination of mastitis and mastitis-causing organisms, including inter alia gram positive and gram negative bacteria, yeasts, fungi and rickettsias. The antimicrobial or anti-inflammatory materials may include inter alia beta lactam antibiotics such as penicillins and cephalosporins, beta lactam antibiotics potentiated by beta lactamase inhibitors such as clavulanic acid, polymyxins, glycopeptides, aminoglycosides, lincosamides, macrolides, pleuromutilins, "fenicols" such as chloramphenicol and florfenicol, tetracyclines, sulphonamides and potentiated sulphonamides such as mixtures of trimethoprim and one or more sulphonamide, quinolones and fluoroquinolones, ionophores, coumarins such as novobiocin, natural or synthetic peptides, lantibiotics, and other antimicrobial products of bacteria and other micro-organisms. Other antimicrobials may be selected from any one or more of macrolide, lincosamide or pleuromutilin, erythromycin, spiramycin, tylosin, spiramycin, tilmicosin, lincomycin, spectinomysin, pirlimycin, tiamulin, potentiated sulphonamide mixtures, trimethoprim plus sulphadiazine, sulphadimidine, sulphadoxine, sulphadimethoxine or other sulphonamide, oxytetracycline, minocycline or doxyclycline, fluoroquinolones, enrofloxacin, ciprofloxacin, norfloxacin, danofloxacin, difloxacin or marbofloxacin. The second component may be selected from any one or more of anti-inflammatory compounds, steroids such as prednisolone, betamethazone, dexamethasone, phenylbutazone, or non-steroids such as flunixin, ketoprofen, carprofen, vedaprofen, meloxicam, tepoxalin, eltenac, nimesulide or tolfenamic acid. Other antimicrobial or anti-inflammatory compounds used in the treatment of intramammary infections in non-human animals may also be used. WO 2005/072644 PCT/IE2005/000007 16 These antimicrobial or anti-inflammatory materials may be formulated either singly or in combinations of two or more compounds as liquids, cerates, solutions, suspensions emulsions or flowable powders in water, oil (of animal, vegetable, mineral or other origin) or other organic vehicles. Other excipients such as solubilising, suspending or emulsifying agents, viscosity modifiers, surfactants, encapsulating agents and other means to adjust the rate at which the compound(s) is released from the formulation, buffers and such agents to maintain the pH of the formulation, anti-inflammatory agents such as various steroidal and non-steroidal compounds commonly used for this purpose, and various preservative agents commonly used in pharmaceutical preparations. Referring to the drawings an initially to Figs. 1 to 8 there is illustrated an injector device 1 according to the invention. The injector device 1 in this case comprises an inner barrel 2 and an outer barrel 3. The outer barrel 3 has a nozzle 4. The inner barrel 2 contains a first component comprising a seal 9. The barrel 2 has a barrier or membrane 5 at its distal end. A plunger 6 is inserted into proximal end of the inner barrel 2 above the seal component 9. An antimicrobial or anti-inflammatory component 10 is contained within the outer barrel 3 below the inner barrel 2. In use, the nozzle 4 is inserted into a teat canal 20 of a non-human animal such as a cow. The inner barrel 2 is pushed through the outer barrel 3 by the plunger 6 to expel the antimicrobial or anti-inflammatory component 10 (Fig. 8(a)). When the antimicrobial or anti-inflammatory component 10 has been expelled (Fig. 8(b)) the plunger 6 on the inner barrel 2 is further depressed to expel the seal 9 from the inner barrel 2. The pressure of the plunger 6 may be sufficient to release or rupture the barrier/membrane 5 on the inner barrel 2 allowing the seal 9 to be expelled from the injector device through the nozzle 4 and into the teat canal (Fig. 8(c)). Rupturing means such as teeth 30 situated within the outer barrel 3 adjacent to the outlet nozzle 4, may be used to release or rupture or open the barrier/membrane 5. Referring to Figs. 9 and 9 (a) there is illustrated another injector which is similar to the injector of Figs. 1 to 8 and like parts are assigned the same reference numerals. WO 2005/072644 PCT/IE2005/000007 17 The injector device 1 comprises an inner barrel 2 and an outer barrel 3. The outer barrel 3 has a nozzle 4. In this case the barrier is released by an activator defined by a number of spikes 50 projecting upwardly to different lengths. The membrane/barrier has a portion 51 which is knocked-out by the spikes 50 to allow ejection of the seal component. The distal end of the inner barrel 2 has a seal or frill 53 which seals with the inner wall of the outer barrel 3. Referring to Figs. 10 to 13 there is illustrated a further injector which is again similar to that of Figs. 1 to 8 and like parts are assigned the same reference numerals. The injector device 1 comprises an inner barrel 2 and an outer barrel 3. The outer barrel 3 has a nozzle 4. In this case the barrier comprises a valve 60 at the distal end of the inner barrel 2. An activator 61 projects upwardly from the lower wall of the outer barrel. In use, when the inner barrel 2 is in the configuration of Fig. 11 the valve 60 is lifted by engagement with the activator 61 and allows the seal component 9 in the inner barrel 2 to pass through the injector nozzle 4. When the plunger 6 is pushed down, the inner barrel 2 also travels down through the outer barrel 3. The outside of the inner barrel 2 is a close fit in the outer barrel 3 so that the inner barrel 2 itself acts as a delivery device or plunger for delivery of the first component from the outer barrel 3 through the nozzle 4. Referring to Figs. 14 to 16 there is illustrated another injector which is again similar to the injector of Figs. 1 to 13 and like parts are assigned the same reference numerals. The injector device comprises an inner barrel 2 and an outer barrel 3. The outer barrel 3 has a nozzle 4. The inner barrel 2 contains a seal component 9. A plunger 6 is inserted into proximal end of the inner barrel 2 above the seal component 9. Antimicrobial 10 is contained within the outer barrel 3. In use, the nozzle 4 is inserted into a teat canal 20. The inner barrel 2 comprises a distal end 74 having an outlet 75 and the barrier comprises a valve 70 which is received in the outlet 75. The valve 70 is normally closed (Figs. 14(a), (b)) during delivery of the first component from the outer barrel 3. An activator 71 projects upwardly from the WO 2005/072644 PCT/IE2005/000007 18 lower wall of the outer barrel 3. In use, when all of the first component has been delivered and the inner barrel 2 is in the configuration of Fig. 14(c) the valve 70 is released by engagement with the activator 71 and the seal component 9 in the inner barrel 2 is allowed to pass through side passageways 76 in valve through the outlet 75 and into the injector nozzle 4. The inner wall 6f the inner barrel 2 is formed for engagement with the plunger 6 and comprises a locking ring 173 between the proximal and distal ends thereof. The inner barrel 2 comprises a distal portion 178 and a proximal portion 179, the distal portion 178 having an internal diameter less than the internal diameter of the proximal portion 179. The plunger 6 comprises a seal 77 which passes over the locking ring 173 of the inner barrel on assembly and the plunger 6 is thus sealingly engaged with the inner barrel 2. The inner barrel 2 further comprises an external seal 72 for engagement with the internal wall of outer barrel 3. The external seal 72 may for example comprise an integrally formed seal or an o-ring housed in a recess 172 in the external side wall of the inner barrel 2. The external seal 72 is located near the distal end 74 of the inner barrel. The internal wall of the outer barrel 3 is formed for engagement with the inner barrel 2 and comprises a. locking ring 73 between the proximal and distal ends thereof for engagement with the external seal 72 of the inner barrel 2. The outer barrel 3 comprises a distal, portion 78 and a proximal portion 79. The distal portion 78 having an internal diameter less than the internal diameter of the proximal portion 79. The external seal 72 passes over locking ring 73 of the outer barrel 3 on assembly and sealingly engages the outer barrel. The antimicrobial or anti-inflammatory formulation 10 is thus prevented from passing between the barrels as the plunger 6 is depressed. WO 2005/072644 PCT/IE2005/000007 19 The valve 70 comprises a plurality of channels 76 which are exposed when the valve 70 is released to allow the seal component to pass out through the valve. Thus upon releasing of the valve the channels 76 which define a passageway for the seal componenfare opened. The activator 71 defines a substantially cylindrical form defining a passageway and corresponds in form to the outlet 75 enabling it to be received in a portion of the outlet. The activator 71 is a close fit with the outlet 75. In use the inner barrel 2 is pushed through the outer barrel 3 by the plunger 6 to expel the antimicrobial 10 (Fig. 14(a)). The outside of the inner barrel 2 has the geometry of the plunger 6 so that the inner barrel 2 itself acts as a plunger. When the antimicrobial 10 has been expelled (Fig. 14(b)) the plunger 6 on the inner barrel 2 is further depressed to expel the seal 9 from the inner barrel 2. The valve 70 is released by engagement of the inner barrel with the activator 71 and the seal component 9 in the inner barrel 2 is allowed to pass through the valve passageways into the injector nozzle 4 and is expelled into the teat canal (Fig. 14(c)). Referring to Figs. 14 to 16, and in particular Figs. 16 (b) and (c) the operation of the valve is described in more details. When closed the valve 70 which is a close fit with the outlet 75 rests in the outlet and prevents any mixing of the components in the outer and inner barrels (Fig. 14(b) and 16(b)). When the activator 71 is received in the outlet 75 (Figs. 14(c) and 16(c)) the valve is released and the passageways 76 in the valve are opened to enable the seal component to pass into the valve passageways 76 through the passageway defined by the activator 71 and into the injector nozzle 4. WO 2005/072644 PCT/IE2005/000007 20 The engagement of the activator 71 with the outlet of the inner barrel 2 provides for alignment of the respective passageways 76 of the valve and the activator before the valve is released and the seal component is allowed to pass from the inner barrel. The respective passageways 76 of the valve and activator provide a closed and 6 isolated pathway for the seal component to pass from the inner barrel 2 directly into the injector nozzle 4. One advantage of a valve type barrier of this embodiment is that there is not risk of any part of the barrier becoming mobile. The barrier is retained with the injector. Referring to Figs. 17 to 18 there is illustrated an injector device 81 of an alternative embodiment of the invention (which is similar to the injector of Figs. 1 to 16 and like parts are assigned the same reference numerals). The injector device 81 comprises a barrel 3, an outlet nozzle 4 and a plunger 6. Two incompatible components 9 and 10 are placed within the barrel 3 of the injector device 81. The two components such as an antimicrobial 10 and a seal 9 are separated from one another by a barrier/membrane. The antimicrobial 10 is placed in the barrel 3 and a seal 9 is placed in a receptacle 86 such as a bag which is defined by an outer membrane 87 which provides the barrier. The receptacle 86 may comprise a capsule into which the seal is filled on manufacture. The capsule may then be readily dropped into the barrel 3 of the injector before a plunger 6 is inserted. When the nozzle 4 of the device 81 is inserted into a teat canal 16 a user depresses the plunger 6 to effect delivery of the antimicrobial 10 from the injector device into the teat canal (Figs. 17(a)). After the antimicrobial 10 is expelled from the injector device further pressure applied to the plunger results in puncturing or bursting of the receptacle 86 allowing egress of the seal 9 which is delivered into the teat canal (Fig. 17(b)). Fig. 17(d) shows the position of a seal formulation 9 and antimicrobial formulation 8 on delivery into the teat of a non-human animal. WO 2005/072644 PCT/IE2005/000007 21 The injector device may comprise rupturing means such as sharp teeth 88 at the distal end of the barrel 3 to assist in puncturing or bursting the receptacle 86. It will be appreciated that for ease of manufacture and use, the receptacle 86 may be attached to the plunger 6. In this case the receptacle is unable to come into contact with the rupturing device until substantially all of the first component has been expelled from the device. When delivering a seal 9 and antimicrobial or anti-inflammatory formulation 10 into the teat canal of a non-human animal the injector device is typically positioned vertically below the teat with the delivery nozzle uppermost. The seal formulation 9 has a much higher specific gravity than the antimicrobial or anti-inflammatory formulation 10 and therefore the receptacle containing the seal formulation remains at the lower end of the barrel 3 containing the antimicrobial or anti-inflammatory formulation during the delivery of the antimicrobial or anti-inflammatory formulation into the teat. In this case the receptacle comes into contact with the rupturing device only when substantially all of the antimicrobial or anti-inflammatory formulation has been delivered into the teat. Referring to Figs. 19 and 20 there is illustrated another injector device 90 of the invention. The injector device 90 comprises an inner barrel 92 and an outer barrel 93. The outer barrel 93 has a nozzle 94. The inner barrel 92 comprises a breakable/burstable barrier or membrane at its distal end and a plunger 98 at its proximal end. Antimicrobial 10 is contained within the outer barrel 93. In use, the nozzle 94 is inserted into a teat canal 16. The inner barrel 92 is pushed through the outer barrel 93 to expel the antimicrobial (Fig. 20(b)). This procedure may be facilitated by a flange 97 around the proximal end of the inner barrel 92 and a flange 99 around the proximal end of the outer barrel 93. When the antimicrobial 10 has been expelled (Fig. 20(c)) the plunger 98 on the inner barrel 92 is depressed to expel the seal 9 from the inner barrel 91. The pressure of the plunger 98 is sufficient to WO 2005/072644 PCT/IE2005/000007 22 burst or rupture the barrier/membrane 96 on the inner barrel 92 allowing the seal 9 to be expelled from the injector device through the nozzle 94 and into the teat canal (Fig. 20(c)). Alternatively rupturing means such as sharp teeth situated within the outer barrel 93 at its aperture into the nozzle, ruptures the barrier/membrane 96. A seal 96 may be provided at the front end of the inner injector barrel 92 to provide a positive seal. The inner injector may be moulded with a weak burstable barrier/membrane 95 across the outlet aperture. It will be appreciated that the seal portion of the formulation may be manufactured in one facility and subsequently combined with the anti-bacterial portion of the formulation at a later stage, in the same or another facility. The invention is not limited to the embodiments hereinbefore described which may be varied in detail. WO 2005/072644 PCT/IE2005/000007 23 Claims 1. Use of a single delivery device for treating or preventing or suppressing a disease or condition in a non-human animal comprising the steps of: - providing a single delivery device containing two components for sequential delivery from the delivery device; delivering a first component from the single delivery device into a teat canal of a non-human animal; and subsequently delivering the second component from the single delivery device into the teat canal without substantial mixing of the components. 2. Use as Iclaimed in claim 1 wherein the delivery device comprises an injector device containing the two components, the components being separated by a barrier and the method comprises the steps of: - delivering the first component from the injector device; at least partially releasing the barrier; and subsequently delivering the second component from the injector device. 3. Use as claimed in claim 1 or 2 wherein the disease or condition is mastitis. 4. Use as claimed in claim 1 or 2 for treating or preventing a mastitis-causing micro-organism. WO 2005/072644 PCT/IE2005/000007 24 5. Use as claimed in any of claims 1 to 4 wherein the second component comprises a seal. 6. Use of a single injector device for treating preventing or suppressing mastitis or a mastitis causing micro-organism comprising the steps of sequentially delivering from a single delivery device an antimicrobial formulation and a seal formulation into the teat canal of a non-human animal wherein the antimicrobial formulation and the seal formulation are delivered into the teat canal without substantial mixing of the formulations prior to delivery into the teat canal. 7. Use as claimed in claim 5 or 6 wherein the seal formulation comprises a non- toxic heavy metal salt. 8. Use as claimed in claim 7 wherein the seal formulation comprises greater than 40% by weight of the heavy metal salt. 9. Use as claimed in claim 7 or 8 wherein the seal formulation comprises between 50% and 75% by weight of the heavy metal salt. 10. Use as claimed in claims 7 to 9 wherein the seal formulation comprises approximately 65% by weight of the heavy metal salt. 11. Use as claimed in claims 7 to 10 wherein the heavy metal is bismuth. 12. Use as claimed in claims 7 to 11 wherein the salt is a sub-nitrate salt. 13. Use as claimed in claims 7 to 12 wherein the seal formulation comprises a gel base. WO 2005/072644 PCT/IE2005/000007 25 14. Use as claimed in claim 13 wherein the gel base is a gel based on aluminium stearate. 15. Use as claimed in claim 13 or 14 wherein the gel base includes liquid paraffin as a vehicle. 16. Use as claimed in claims 1 to 5 or 7 to 15 wherein the first component comprises an antimicrobial. 17. Use as claimed in claim 6 or 16 wherein the antimicrobial is selected from any one or more of betalactam antibiotics, polymyxins, glycopeptides, aminoglycosides, lincosamides, macrolides, pleuromutilins, "fenicols" such as chloramphenicol and florfenicol, tetracylcines, sulphonamides and potentiated sulphonamides such as mixtures of trimethoprim and one or more sulphonamide, quinolones and fluoroquinolones, ionophores, courmarins such as novobiocin, natural or synthetic peptides, aminoglycosides, antimicrobial peptides or antimicrobials, lantibiotics, or other products of bacteria and other micro-organisms. 18. Use as claimed in claim 17 wherein the betalactam is selected from any one or more of penicillin, modified penicillin such as cloxacillin, amoxycillin, ampicillin, cephalosporins or beta lactam antibiotics potentiated by beta lactamase inhibitors such as clavulanic acid. 19. Use as claimed in claim 17 wherein the aminoglycoside is selected from any one or more of streptomycin, dihydrostreptomycin, neomycin, gentamycin, framycetin, aparamycin or kanamycin. 20. Use as claim 6 or 16 wherein the antimicrobial is selected from any one or more of macrolide, lincosamide or pleuromutilin, erythromycin, spiramycin, WO 2005/072644 PCT/IE2005/000007 26 tylosin, spiramycin, tilmicosin, lincomycin, spectinomysin, pirlimycin or tiamulin. 21. Use as claimed in claim 6 or 16 wherein the antimicrobial is selected from any one or more of potentiated sulphonamide mixtures, trimethoprim plus sulphadiazine, sulphadimidine, sulphadoxine, sulphadimethoxine or other sulphonamide, oxytetracycline, minocycline or doxyclycline, fiuoroquinolones, enrofloxacin, ciprofloxacin, norfloxacin, danofioxacin, difloxacin or marbofloxacin. 22. Use of claim 1 to 5 or 7 to 15 wherein the first component comprises an anti- inflammatory. 23. Use as claimed in claim 22 wherein the anti-inflammatory is selected from any one or more of steroids such as prednisolone, betamethazone, dexamethazone, phenylbutazone, or non-steroids such as flunixin, ketoprofen, carprofen, vedaprofen, meloxicam, tepoxalin, eltenac, nimesulide or tolfenamic acid. 24. Use substantially as hereinbefore described with reference to the drawings. 25. An injector device for delivery of components into the teat canal of a non- human animal comprising: a barrel for containing a first component, an outlet nozzle at one end of the barrel, an internal receptacle for containing a second component, a barrier for separating a first component and a second component, and WO 2005/072644 PCT/1E20O5/0OO007 27 a delivery means for delivery of a first component from the barrel and sequential delivery of a second component from the internal receptacle through the outlet nozzle. 26. An injector device as claimed in claim 25 wherein the barrier is normally closed. 27. An injector device as claimed in claims 25 or 26 wherein the barrier is releasable for delivery of the second component. 28. An injector device as claimed in claims 25 to 27 wherein the barrier is defined by at least a portion of the internal receptacle. 29. An injector-device as claimed in claims 25 to 28 wherein the barrier comprises one or more passageways. 30. An injector device as claimed in claim 29 wherein the one or more passageways are opened when the barrier is released for delivery of the second component through said one or more passageways. 31. An injector device as claimed in claims 25 to 30 wherein the device comprises an activator, for releasing the barrier. 32. An injector as claimed in claim 31 wherein the activator comprises a mechanical activator means. 33. An injector as claimed in claims 31 or 32 wherein the activator comprises at least one projecting member. WO 2005/072644 PCT/IE2005/000007 28 34. An injector as claimed in any of claims 31 to 33 wherein the activator is located in the barrel. 35. An injector as claimed in any of claims 31 to 34 wherein the activator is located adjacent to the outlet nozzle. 36. An injector device as claimed in claims 31 to 35 wherein the activator comprises one or more passageways. 37. An injector device as claimed in claims 31 to 36 wherein the activator is configured for engagement with the internal receptacle to provide a direct passageway for delivery of the second component from the internal receptacle into the outlet nozzle. 38. An injector; as claimed in any of claims 25 to 37 wherein the delivery means comprises a plunger for the barrel. 39. An injector: device as claimed in any of claims 25 to 38 wherein the barrier is released by ;the delivery means. 40. An injectorsas claimed in any of claims 25 to 39 wherein the internal receptacle comprises an inner barrel located within an outer barrel defined by the barrel of the injector. 41. An injector as claimed in claim 40 wherein the inner barrel is a close fit within the outer barrel. 42. An injector as claimed in any of claims 40 or 41 wherein the delivery means comprises the inner barrel. WO 2005/072644 PCT/IE2005/000007 29 43. An injector as claimed in claims 40 to 42 wherein the inner barrel defines a plunger for the outer barrel. 44. An injector as claimed in any of claims 40 to 43 wherein the delivery means comprises a plunger for the inner barrel. 45. An injector as claimed in claims 40 to 44 wherein the inner barrel comprises engagement means for engagement with the outer barrel on assembly. 46. An injector as claimed in claim 45 wherein the engagement means comprises an external seal. 47. An injector as claimed in any of claims 45 or 46 wherein the outer barrel comprises engagement means for engagement with the inner barrel. 48. An injector a's claimed in claim 47 wherein the outer barrel comprises a locking ring for engagement with the inner barrel. 49. An injector as claimed in any of claims 40 to 48 wherein the inner barrel comprises engagement means for engagement with the plunger. 50. An injector as claimed in claim 49 wherein the inner barrel comprises a locking ring for engagement with the plunger. 51. An injector device as claimed in any of claims 25 to 39, wherein the receptacle comprises a bag. 52. An injector device as claimed in any of claims 25 to 39, wherein the receptacle comprises a capsule. WO 2005/072644 PCT/IE2005/000007 30 53. An injector as claimed in any of claims 51 or 52 wherein the receptacle is attached to or forms an integral part of the delivery means. 54. An injector device as claimed in any of claims 31 to 53 wherein the activator comprises a rupturing means for at least partially releasing the barrier. 55. An injector as claimed in claim 54 wherein the rupturing means comprises a mechanical rupture member. 56. An injector as claimed in claim 55 wherein the rupture member comprises at least one blade. 57. An injector as claimed in claims 55 or 56 wherein the rupture member comprises at least one tooth. 58. An injector! as claimed in any of claims 55 to 57 wherein the rupture member is located in the barrel. 59. An injector as claimed in any of claims 55 to 58 wherein the rupture member is located adjacent to the outlet nozzle. 60. An injector device as claimed in any of claims 25 to 59 wherein the barrel contains a first component. 61. An injector device as claimed in claim 60 wherein the first component comprises an antimicrobial formulation. 62. An injector device as claimed in any of claims 25 to 61 wherein the internal receptacle contains a second component. WO 2005/072644 PCT/IE2005/000007 31 63. An injector device as claimed in claim 62 the second component comprises a seal formulation. 64. An injector device as claimed in any of claims 25 to 63 wherein a first component is delivered from the barrel and a second component is subsequently delivered from the internal receptacle without substantial mixing of the components. 65. An injector device as substantially hereinbefore described with reference to the accompanying drawings. 66. A method for treating or preventing or suppressing a disease or condition in a non-human animal comprising the steps of: - providing a single delivery device containing two components for sequential delivery from the delivery device; delivering a first component from the single delivery device into a teat canal of anon-human animal; and subsequently delivering the second component from the single delivery device into the teat canal without substantial mixing of the components. 67. A method as claimed in claim 66 wherein the delivery device comprises an injector device containing the two components, the components being separated by a barrier and the method comprises the steps of: - delivering the first component from the injector device; at least partially releasing the barrier; and WO 2005/072644 PCT/IE2005/000007 32 subsequently delivering the second component from the injector device. 68. A method as claimed in claim 66 or 67 wherein the disease or condition is mastitis. 69. A method as claimed in claim 66 or 67 for treating or preventing a mastitis- causing?micro-organism. 70. A method as claimed in any of claims 66 to 69 wherein the second component comprises a seal. 71. A method for treating, preventing or suppressing mastitis or a mastitis causing micro-organism comprising the steps of sequentially delivering from a single delivery device an antimicrobial formulation and a seal formulation into the teat canal of a non-human animal wherein the antimicrobial formulation and the seal formulation are delivered into the teat canal without substantial mixing of the formulations prior to delivery into the teat canal. 72. A method as claimed in claim 70 or 71 wherein the seal formulation comprises a non-toxic heavy metal salt. 73. A method as claimed in claim 72 wherein the seal formulation comprises greater than 40% by weight of the heavy metal salt. 74. A method as claimed in claim 72 or 73 wherein the seal formulation comprises between 50% and 75% by weight of the heavy metal salt. 75. A method as claimed in any of claims 72 to 74 wherein the seal formulation comprises approximately 65% by weight of the heavy metal salt. WO 2005/072644 PCT/IE2005/000007 33 76. A method as claimed in any of claims 72 to 75 wherein the heavy metal is bismuth. 77. A method as claimed in any of claims 72 to 75 wherein the salt is a sub- nitrate salt. 78. A method as claimed in any of claims 70 to 77 wherein the seal formulation comprises a gel base. 79. A method as claimed in claim 78 wherein the gel base is a gel based on aluminium stearate. 80. A method as claimed in claim 78 or 79 wherein the gel base includes liquid paraffin as a vehicle. 81. A method as claimed in any of claims 66 to 70 or 72 to 80 wherein the first component comprises an antimicrobial. 82. A method as claimed in claim 71 or 81 wherein the antimicrobial is selected from any one or more of betalactam antibiotics, polymyxins, glycopeptides, aminoglycosides, lincosamides, macrolides, pleuromutilins, "fenicols" such as chloraniphenicol and florfenicol, tetracylcines, sulphonamides and potentiated sulphonamides such as mixtures of trimethoprim and one or more sulphonamide, quinolones and fluoroquinolones, ionophores, courmarins such as novobiocin, natural or synthetic peptides, aminoglycosides, antimicrobial peptides or antimicrobials, lantibiotics, or other products of bacteria and other micro-organisms. 83. A method as claimed in claim 82 wherein the betalactam is selected from any one or more of penicillin, modified penicillin such as cloxacillin, amoxycillin, WO 2005/072644 PCT/IE2005/000007 34 ampicillin., cephalosporins or beta lactam antibiotics potentiated by beta lactamase inhibitors such as clavulanic acid. 84. A method as claimed in claim 82 wherein the aminoglycoside is selected from any one or more of streptomycin, dihydrostreptomycin, neomycin, gentamycin, framycetin, aparamycin or kanamycin. 85. A method as claimed in claim 71 or 81 wherein the antimicrobial is selected from any one or more of macrolide, lincosamide or pleuromutilin, erythromycin, spiramycin, tylosin, spiramycin, tilmicosin, lincomycin, spectinomysin, pirlimycin or tiamulin. 86. A method as claimed in claim 71 or 81 wherein the antimicrobial is selected from any one or more of potentiated sulphonamide mixtures, trimethoprim plus sulphadiazine, sulphadimidine, sulphadoxine, sulphadimethoxine or other sulphonamide, oxytetracycline, minocycline or doxyclycline, fluoroquinolones, enrofloxacin, ciprofloxacin, norfloxacin, danofloxacin, difloxacin or marbofloxacin. 87. A method as claimed in any of claims 66 to 70 to 72 to 80 wherein the first component comprises an anti-inflammatory. 88. A method as claimed in claim 87 wherein the anti-inflammatory is selected from any one or more of steroids such as prednisolone, betamethazone, dexamethazone, phenylbutazone, or non-steroids such as flunixin, ketoprofen, carprofen, vedaprofen, meloxicam, tepoxalin, eltenac, nimesulide or tolfenamic acid. 89. A method substantially as hereinbefore described with reference to the accompanying drawings. The invention relates to a method and device for treating or preventing or suppressing a disease or condition in a non-human animal. The method comprises the steps of providing a single delivery device containing two components for sequential delivery from the delivery device. A first component is delivered from the single delivery device into a teat canal of a non-human animal and subsequently the second component is delivered from the single delivery device into the teat canal. The components are delivered without substantial mixing of the components.

Full Text

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METHOD AND DEVICE FOR TREATING A TEAT CANAL OF AN ANIMAL
Introduction
The invention relates to a method and device for administering two components into the teat canal of a non-human animal.
Bovine mastitis is a severe, potentially fatal, inflammatory disease of the udder, caused by a broad range of infectious organisms, but most notably by various Gram positive bacteria of the genera Staphylococcus and Streptococcus and the Gram negative species, Escherichia coli. The infection usually enters the udder via the teat or streak canal. Mastitis is treated by a variety of antibiotic cerates, infused into the udder via the teat or streak canal. In severe cases, high doses of antibiotic are also given by injection. A high proportion of mastitic infections are contracted during the "dry" period, which precedes calving. The infection may later become clinically significant either during the dry period, or after calving when lactation has resumed.
It is known to treat mastitis using a twin injector pack, one injector containing an anti-bacterial formulation and a second injector containing a seal or barrier formulation. The anti-bacterial formulation is delivered first to the teat canal followed by delivery of the seal formulation forming a physical barrier in the teat canal against the entry of bacteria into the udder. These twin injector packs are sold under the name Teat Seal™. WO94/13261 and WO95/31180 describe the twin injectors in detail and are hereby incorporated by reference.
While the twin injector system provides an effective method to reduce the incidence of clinical mastitis administration of the injectors can be time consuming, doubles the risk of introducing extraneous environmental bacteria and doubles the risk of causing damage to the epithelium of the streak canal. The use of two injectors also increases the cost of treatment and creates additional non-degradable waste.

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There is a need for an improved method and device for preventing intrammary disorders which will overcome at least some of these problems.
Statements of Invention
According to another aspect of the invention there is provided use of a single delivery device for treating or preventing or suppressing a disease or condition in a non-human animal comprising the steps of: -
providing a single delivery device containing two components for sequential delivery from the delivery device;
delivering a first component from the single delivery device into a teat canal of anon-human animal; and
subsequently delivering the second component from the single delivery device into the teat canal without substantial mixing of the components.
In one embodiment the delivery device comprises an injector device containing the two components, the components being separated by a barrier and the method comprises the steps of: -
delivering the first component from the injector device; at least partially releasing the barrier; and
subsequently delivering the second component from the injector device.

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In another embodiment the disease or condition is mastitis and the use is for treating or preventing a mastitis-causing micro-organism.
In one embodiment the second component comprises a seal.
According to another aspect of the invention there is provided use of a single injector device for treating preventing or suppressing mastitis or a mastitis causing microorganism comprising the steps of sequentially delivering from a single delivery device an antimicrobial formulation and a seal formulation into the teat canal of a non-human animal wherein the antimicrobial formulation and the seal formulation are delivered into the teat canal without substantial mixing of the formulations prior to delivery into the teat canal.
In one embodiment the seal formulation comprises a non-toxic heavy metal salt.
In another embodiment the seal formulation comprises greater than 40% by weight of the heavy metal salt.
In a further embodiment the seal formulation comprises between 50% and 75% by weight of the heavy metal salt.
In one embodiment the seal formulation comprises approximately 65% by weight of the heavy metal salt.
In another embodiment the heavy metal is bismuth.
In a further embodiment the salt is a sub-nitrate salt.
In one embodiment the seal formulation comprises a gel base.
In another embodiment the gel base is a gel based on aluminium stearate.

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In a further embodiment the gel base includes liquid paraffin as a vehicle. In one embodiment the first component comprises an antimicrobial.
In another embodiment the antimicrobial is selected from any one or more of betalactam antibiotics, polymyxins, glycopeptides, aminoglycosides, lincosamides, macrolides, pleuromutilins, "fenicols" such as chloramphenicol and florfenicol, tetracylcines, sulphonamides and potentiated sulphonamides such as mixtures of trimethoprim and one or more sulphonamide, quinolones and fluoroquinolones, ionophores, courmarins such as novobiocin, natural or synthetic peptides, aminoglycosides, antimicrobial peptides or antimicrobials, lantibiotics, or other products of bacteria and other micro-organisms.
In a further embodiment the betalactam is selected from any one or more of penicillin, modified penicillin such as cloxacillin, amoxycillin, ampicillin, cephalosporins or beta lactam antibiotics potentiated by beta lactamase inhibitors such as clavulanic acid.
In one embodiment the aminoglycoside is selected from any one or more of streptomycin, dihydrostreptomycin, neomycin, gentamycin, framycetin, aparamycin or kanamycin.
In another embodiment the antimicrobial is selected from any one or more of macrolide, lincosamide or pleuromutilin, erythromycin, spiramycin, tylosin, spiramycin, tilmicosin, lincomycin, spectinomysin, pirlimycin or tiamulin.
In a further embodiment the antimicrobial is selected from any one or more of potentiated sulphonamide mixtures, trimethoprim plus sulphadiazine, sulphadimidine, sulphadoxine, sulphadimethoxine or other sulphonamide,

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oxytetracycline, minocycline or doxyclycline, fluoroquinolones, enrofloxacin, ciprofloxacin, norfloxacin, danofloxacin, difloxacin or niarbofloxacin.
In one embodiment the first component comprises an anti-inflammatory.
In another embodiment the anti-inflammatory is selected from any one or more of steroids such as prednisolone, betamethazone, dexamethazone, phenylbutazone, or non-steroids such as fiunixin, ketoprofen, carprofen, vedaprofen, meloxicam, tepoxalin, eltenac, nimesulide or tolfenamic acid.
According to further aspect of the invention there is provided an injector device for delivery of components into the teat canal of a non-human animal comprising:
a barrel for containing a first component,
an outlet nozzle at one end of the barrel,
an internal receptacle for containing a second component,
a barrier for separating a first component and a second component, and
a delivery means for delivery of a first component from the barrel and sequential delivery of a second component from the internal receptacle through the outlet nozzle.
In one embodiment the barrier is normally closed.
In another embodiment the barrier is releasable for delivery of the second component.

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In a further embodiment the barrier is defined by at least a portion of the internal receptacle.
In one embodiment the barrier comprises one or more passageways.
In another embodiment the one or more passageways are opened when the barrier is released for delivery of the second component through said one or more passageways.
In a further embodiment the device comprises an activator for releasing the barrier.
In one embodiment the activator comprises a mechanical activator means.
In another embodiment the activator comprises at least one projecting member.
In a further embodiment the activator is located in the barrel.
In one embodiment the activator is located adjacent to the outlet nozzle.
In another embodiment the activator comprises one or more passageways.
In a further embodiment the activator is configured for engagement with the internal receptacle to provide a direct passageway for delivery of the second component from the internal receptacle into the outlet nozzle.
In one embodiment the delivery means comprises a plunger for the barrel, In another embodiment the barrier is released by the delivery means.
In a further embodiment the internal receptacle comprises an inner barrel located within an outer barrel defined by the barrel of the injector.

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In one embodiment the inner barrel is a close fit within the outer barrel.
In another embodiment the delivery means comprises the inner barrel.
In a further embodiment the inner barrel defines a plunger for the outer barrel.
In one embodiment the delivery means comprises a plunger for the inner barrel.
In another embodiment the inner barrel comprises engagement means for engagement with the outer barrel on assembly.
In a further embodiment the engagement means comprises an external seal.
In one embodiment the outer barrel comprises engagement means for engagement with the inner barrel.
In another embodiment the outer barrel comprises a locking ring for engagement with the inner barrel.
In a further embodiment the inner barrel comprises engagement means for engagement with the plunger.
In one embodiment the inner barrel comprises a locking ring for engagement with the plunger.
In another embodiment the receptacle comprises a bag.
In a further embodiment the receptacle comprises a capsule.

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In one embodiment the receptacle is attached to or forms an integral part of the delivery means.
In another embodiment the activator comprises a rupturing means for at least partially releasing the barrier.
In a further embodiment the rupturing means comprises a mechanical rupture member.
In one embodiment the rupture member comprises at least one blade.
In another embodiment the rupture member comprises at least one tooth.
In a further embodiment the rupture member is located in the barrel.
In one embodiment the rupture member is located adjacent to the outlet nozzle.
In another embodiment the barrel contains a first component.
In a further embodiment the first component comprises an antimicrobial formulation.
In one embodiment the internal receptacle contains a second component.
In another embodiment the second component comprises a seal formulation.
In a further embodiment a first component is delivered from the barrel and a second component is subsequently delivered from the internal receptacle without substantial mixing of the components.

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In one embodiment the seal formulation may comprise the seal formulation as described above.
In another embodiment the antimicrobial formulation may comprise the antimicrobial formulation as described above.
According to the invention there is provided a method for treating or preventing or suppressing a disease or condition in a non-human animal comprising the steps of: -
providing a single delivery device containing two components for sequential delivery from the delivery device;
delivering a first component from the single delivery device into a teat canal of a non-human animal; and
subsequently delivering the second component from the single delivery device into the teat canal without substantial mixing of the components.
In one embodiment the delivery device comprises an injector device containing the two components, the components being separated by a barrier and the method comprises the steps of: -
delivering the first component from the injector device; at least partially releasing the barrier; and
subsequently delivering the second component from the injector device.

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In another embodiment the disease or condition is mastitis and the method of the invention is for treating or preventing a mastitis-causing micro-organism.
In a further embodiment the second component comprises a seal.
According to a further aspect the invention provides a method for treating, preventing or suppressing mastitis or a mastitis causing micro-organism comprising the steps of sequentially delivering, from a single delivery device an antimicrobial formulation and a seal formulation into the teat canal of a non-human animal wherein the antimicrobial formulation and the seal formulation are delivered into the teat canal without substantial mixing of the formulations prior to delivery into the teat canal.
In one embodiment the seal formulation comprises a non-toxic heavy metal salt.
ii
In another embodiment the seal formulation comprises greater than 40% by weight of the heavy metal salt.
In a further embodiment the seal formulation comprises between 50% and 75% by weight of the heavy metal salt.
In one embodiment the seal formulation comprises approximately 65% by weight of the heavy metal salt.
In another embodiment the heavy metal is bismuth.
In a further embodiment the salt is a sub-nitrate salt.
In one embodiment the seal formulation comprises a gel base.
In another embodiment the gel base is a gel based on aluminium stearate.

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In a further embodiment the gel base includes liquid paraffin as a vehicle. In one embodiment the first component comprises an antimicrobial.
In another embodiment the antimicrobial is selected from any one or more of betalactam antibiotics, polymyxins, glycopeptides, aminoglycosides, lincosamides, macrolides, pleiiromutilins, "fenicols" such as chloramphenicol and florfenicol., tetracylcines, sulphonamides and potentiated sulphonamides such as mixtures of trimethoprim and one or more sulphonamide, quinolones and fluoroquinolones, ionophores, courmarins such as novobiocin, natural or synthetic peptides, aminoglycosides, antimicrobial peptides or antimicrobials, lantibiotics, or other products of bacteria and other micro-organisms.
In a further embodiment the betalactam is selected from any one or more of penicillin, modified penicillin such as cloxacillin, amoxycillin, ampicillin, cephalosporins or beta lactam antibiotics potentiated by beta lactamase inhibitors such as clavulanic acid.
In one embodiment the aminoglycoside is selected from any one or more of streptomycin, dihydrostreptomycin, neomycin, gentamycin, framycetin, aparamycin or kanamycin.
In another embodiment the antimicrobial is selected from any one or more of rnacrolide, lincosamide or pleuromutilin, erythromycin, spiramycin, tylosin, spiramycin, tilmicosin, lincomycin, spectinomysin, pirlimycin or tiamulin.
In a further embodiment the antimicrobial is selected from any one or more of potentiated sulphonamide mixtures, trimethoprim plus sulphadiazine, sulphadimidine, sulphadoxine, sulphadimethoxine or other sulphonamide,

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oxytetracycline, minocycline or doxyclycline, fluoroquinolones, enrofloxacin, ciprofloxacin, nprfloxacin, danofloxacin, difloxacin or marbofloxacin.
In one embodiment the first component comprises an anti-inflammatory.
In another embodiment the anti-inflammatory is selected from any one or more of steroids such as prednisolone, betamethazone, dexamethazone, phenylbutazone, or non-steroids such as flunixin, ketoprofen, carprofen, vedaprofen, meloxicam, tepoxalin, eltenac, nimesulide or tolfenamic acid.
Brief description of Drawings
The invention will be more clearly understood from the following description thereof given by way of example only with reference to the accompanying drawings, in which: -
Fig. 1 is a schematic cross sectional view of an injector device according to the invention;
Figs. 2 is a cross sectional view of an internal barrel of the device;
Fig. 3 is a cross sectional view of the internal barrel of Fig. 2 with a seal component in place;
Figs. 4 is a cross sectional view of the internal barrel of Fig. 3 with a plunger inserted;
Fig. 5 is a cross sectional view of an external barrel of the device;

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Fig. 6 is a cross sectional view of the external barrel of Fig. 5 with an antimicrobial component in place;
Fig. 7 is a cross sectional view of the assembled injector device; Figs. 8(a) to 8(c) are cross sectional views of the injector device, in use; Fig. 9 is a cross sectional view of another injector of the invention; Fig. 9(a) is an enlarged view of portion of the injector in Fig. 9;
Figs. 10 to 12 are cross sectional views of a further injector device according to the invention in different configurations of use;
Fig. 13 is a top plan view of the device of Fig. 9;
Figs. 14 (a) to 14 (c) are cross sectional views of a further injector device according to the invention in different configurations of use;
Fig 15 is an exploded broken out view of components of the injector of Fig.14;
Figs. 16 (a) is a detailed cross sectional view of a portion of the injector of Fig. 14, and Figs. 16(b) and 16 (c) are detailed perspective views of a portion of the device in the configurations of Fig. 14 (b) and (c) respectively;
Fig. 17 is a schematic cross sectional view of an injector device according to a further embodiment of the invention;
Figs. 18(a) to 18(d) are cross sectional views of the device of Fig. 17, in use;

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Fig. 19 is a schematic cross sectional view of another injector device according to the invention; and
Figs. 20(a) to 20(c) are cross sectional views of the injector device of Fig. 19, in use.
Detailed Description
The invention provides an injector device that allows for the sequential delivery of two incompatible components such as an antimicrobial formulation and a seal formulation into the teat canal of a non-human animal. The seal formulation and antimicrobial formulation are contained separately within a single injector device. This enables the product to be stored without affecting the stability of either component. The device also provides for the delivery of the antimicrobial formulation ahead of the seal formulation which effectively forms a physical barrier in the teat canal preventing any further entry into the teat canal. The seal formulation also prevents the possibility of the antimicrobial phase leaking or being expressed from the teat by gravitational or hydrostatic forces.
The seal formulation may comprise a viscous oil-based cerate containing a high proportion of a heavy metal salt, bismuth subnitrate. The product Teat Seal (trade mark of Cross Vetpharm Group) is described in detail in WO98/26759 and comprises a non-toxic heavy metal salt in a gel base. The base is a gel based on aluminium stearate. The gel preferably includes a vehicle such as liquid paraffin. The gel may also comprise a polyethylene gel. The gel may be based on low density polyethylene or on high density polyethylene. Preferably, the heavy metal salt is present in an amount of greater than 40%, preferably between 50% and 75% by weight, most preferably approximately 65% by weight.
The seal formulation prevents infection entering the udder via the teat or streak canal through a combination of its viscosity, density and adhesiveness.

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The antimicrobial or anti-inflammatory formulation may be selected from any one or more of a wide variety of compounds that are known to be effective for the treatment, prevention and elimination of mastitis and mastitis-causing organisms, including inter alia gram positive and gram negative bacteria, yeasts, fungi and rickettsias. The antimicrobial or anti-inflammatory materials may include inter alia beta lactam antibiotics such as penicillins and cephalosporins, beta lactam antibiotics potentiated by beta lactamase inhibitors such as clavulanic acid, polymyxins, glycopeptides, aminoglycosides, lincosamides, macrolides, pleuromutilins, "fenicols" such as chloramphenicol and florfenicol, tetracyclines, sulphonamides and potentiated sulphonamides such as mixtures of trimethoprim and one or more sulphonamide, quinolones and fluoroquinolones, ionophores, coumarins such as novobiocin, natural or synthetic peptides, lantibiotics, and other antimicrobial products of bacteria and other micro-organisms.
Other antimicrobials may be selected from any one or more of macrolide, lincosamide or pleuromutilin, erythromycin, spiramycin, tylosin, spiramycin, tilmicosin, lincomycin, spectinomysin, pirlimycin, tiamulin, potentiated sulphonamide mixtures, trimethoprim plus sulphadiazine, sulphadimidine, sulphadoxine, sulphadimethoxine or other sulphonamide, oxytetracycline, minocycline or doxyclycline, fluoroquinolones, enrofloxacin, ciprofloxacin, norfloxacin, danofloxacin, difloxacin or marbofloxacin.
The second component may be selected from any one or more of anti-inflammatory compounds, steroids such as prednisolone, betamethazone, dexamethasone, phenylbutazone, or non-steroids such as flunixin, ketoprofen, carprofen, vedaprofen, meloxicam, tepoxalin, eltenac, nimesulide or tolfenamic acid.
Other antimicrobial or anti-inflammatory compounds used in the treatment of intramammary infections in non-human animals may also be used.

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These antimicrobial or anti-inflammatory materials may be formulated either singly or in combinations of two or more compounds as liquids, cerates, solutions, suspensions emulsions or flowable powders in water, oil (of animal, vegetable, mineral or other origin) or other organic vehicles. Other excipients such as solubilising, suspending or emulsifying agents, viscosity modifiers, surfactants, encapsulating agents and other means to adjust the rate at which the compound(s) is released from the formulation, buffers and such agents to maintain the pH of the formulation, anti-inflammatory agents such as various steroidal and non-steroidal compounds commonly used for this purpose, and various preservative agents commonly used in pharmaceutical preparations.
Referring to the drawings an initially to Figs. 1 to 8 there is illustrated an injector device 1 according to the invention. The injector device 1 in this case comprises an inner barrel 2 and an outer barrel 3. The outer barrel 3 has a nozzle 4. The inner barrel 2 contains a first component comprising a seal 9. The barrel 2 has a barrier or membrane 5 at its distal end. A plunger 6 is inserted into proximal end of the inner barrel 2 above the seal component 9. An antimicrobial or anti-inflammatory component 10 is contained within the outer barrel 3 below the inner barrel 2. In use, the nozzle 4 is inserted into a teat canal 20 of a non-human animal such as a cow. The inner barrel 2 is pushed through the outer barrel 3 by the plunger 6 to expel the antimicrobial or anti-inflammatory component 10 (Fig. 8(a)). When the antimicrobial or anti-inflammatory component 10 has been expelled (Fig. 8(b)) the plunger 6 on the inner barrel 2 is further depressed to expel the seal 9 from the inner barrel 2. The pressure of the plunger 6 may be sufficient to release or rupture the barrier/membrane 5 on the inner barrel 2 allowing the seal 9 to be expelled from the injector device through the nozzle 4 and into the teat canal (Fig. 8(c)). Rupturing means such as teeth 30 situated within the outer barrel 3 adjacent to the outlet nozzle 4, may be used to release or rupture or open the barrier/membrane 5.
Referring to Figs. 9 and 9 (a) there is illustrated another injector which is similar to the injector of Figs. 1 to 8 and like parts are assigned the same reference numerals.

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The injector device 1 comprises an inner barrel 2 and an outer barrel 3. The outer barrel 3 has a nozzle 4. In this case the barrier is released by an activator defined by a number of spikes 50 projecting upwardly to different lengths. The membrane/barrier has a portion 51 which is knocked-out by the spikes 50 to allow ejection of the seal component. The distal end of the inner barrel 2 has a seal or frill 53 which seals with the inner wall of the outer barrel 3.
Referring to Figs. 10 to 13 there is illustrated a further injector which is again similar to that of Figs. 1 to 8 and like parts are assigned the same reference numerals. The injector device 1 comprises an inner barrel 2 and an outer barrel 3. The outer barrel 3 has a nozzle 4. In this case the barrier comprises a valve 60 at the distal end of the inner barrel 2. An activator 61 projects upwardly from the lower wall of the outer barrel. In use, when the inner barrel 2 is in the configuration of Fig. 11 the valve 60 is lifted by engagement with the activator 61 and allows the seal component 9 in the inner barrel 2 to pass through the injector nozzle 4.
When the plunger 6 is pushed down, the inner barrel 2 also travels down through the outer barrel 3. The outside of the inner barrel 2 is a close fit in the outer barrel 3 so that the inner barrel 2 itself acts as a delivery device or plunger for delivery of the first component from the outer barrel 3 through the nozzle 4.
Referring to Figs. 14 to 16 there is illustrated another injector which is again similar to the injector of Figs. 1 to 13 and like parts are assigned the same reference numerals. The injector device comprises an inner barrel 2 and an outer barrel 3. The outer barrel 3 has a nozzle 4. The inner barrel 2 contains a seal component 9. A plunger 6 is inserted into proximal end of the inner barrel 2 above the seal component 9. Antimicrobial 10 is contained within the outer barrel 3. In use, the nozzle 4 is inserted into a teat canal 20. The inner barrel 2 comprises a distal end 74 having an outlet 75 and the barrier comprises a valve 70 which is received in the outlet 75. The valve 70 is normally closed (Figs. 14(a), (b)) during delivery of the first component from the outer barrel 3. An activator 71 projects upwardly from the

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lower wall of the outer barrel 3. In use, when all of the first component has been delivered and the inner barrel 2 is in the configuration of Fig. 14(c) the valve 70 is released by engagement with the activator 71 and the seal component 9 in the inner barrel 2 is allowed to pass through side passageways 76 in valve through the outlet 75 and into the injector nozzle 4.
The inner wall 6f the inner barrel 2 is formed for engagement with the plunger 6 and comprises a locking ring 173 between the proximal and distal ends thereof. The inner barrel 2 comprises a distal portion 178 and a proximal portion 179, the distal portion 178 having an internal diameter less than the internal diameter of the proximal portion 179. The plunger 6 comprises a seal 77 which passes over the locking ring 173 of the inner barrel on assembly and the plunger 6 is thus sealingly engaged with the inner barrel 2.
The inner barrel 2 further comprises an external seal 72 for engagement with the internal wall of outer barrel 3. The external seal 72 may for example comprise an integrally formed seal or an o-ring housed in a recess 172 in the external side wall of the inner barrel 2. The external seal 72 is located near the distal end 74 of the inner barrel.
The internal wall of the outer barrel 3 is formed for engagement with the inner barrel 2 and comprises a. locking ring 73 between the proximal and distal ends thereof for engagement with the external seal 72 of the inner barrel 2. The outer barrel 3 comprises a distal, portion 78 and a proximal portion 79. The distal portion 78 having an internal diameter less than the internal diameter of the proximal portion 79. The external seal 72 passes over locking ring 73 of the outer barrel 3 on assembly and sealingly engages the outer barrel. The antimicrobial or anti-inflammatory formulation 10 is thus prevented from passing between the barrels as the plunger 6 is depressed.

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The valve 70 comprises a plurality of channels 76 which are exposed when the valve 70 is released to allow the seal component to pass out through the valve. Thus upon releasing of the valve the channels 76 which define a passageway for the seal componenfare opened.
The activator 71 defines a substantially cylindrical form defining a passageway and corresponds in form to the outlet 75 enabling it to be received in a portion of the outlet. The activator 71 is a close fit with the outlet 75.
In use the inner barrel 2 is pushed through the outer barrel 3 by the plunger 6 to expel the antimicrobial 10 (Fig. 14(a)). The outside of the inner barrel 2 has the geometry of the plunger 6 so that the inner barrel 2 itself acts as a plunger. When the antimicrobial 10 has been expelled (Fig. 14(b)) the plunger 6 on the inner barrel 2 is further depressed to expel the seal 9 from the inner barrel 2. The valve 70 is released by engagement of the inner barrel with the activator 71 and the seal component 9 in the inner barrel 2 is allowed to pass through the valve passageways into the injector nozzle 4 and is expelled into the teat canal (Fig. 14(c)).
Referring to Figs. 14 to 16, and in particular Figs. 16 (b) and (c) the operation of the valve is described in more details.
When closed the valve 70 which is a close fit with the outlet 75 rests in the outlet and prevents any mixing of the components in the outer and inner barrels (Fig. 14(b) and 16(b)).
When the activator 71 is received in the outlet 75 (Figs. 14(c) and 16(c)) the valve is released and the passageways 76 in the valve are opened to enable the seal component to pass into the valve passageways 76 through the passageway defined by the activator 71 and into the injector nozzle 4.

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The engagement of the activator 71 with the outlet of the inner barrel 2 provides for alignment of the respective passageways 76 of the valve and the activator before the valve is released and the seal component is allowed to pass from the inner barrel. The respective passageways 76 of the valve and activator provide a closed and 6 isolated pathway for the seal component to pass from the inner barrel 2 directly into the injector nozzle 4.
One advantage of a valve type barrier of this embodiment is that there is not risk of any part of the barrier becoming mobile. The barrier is retained with the injector.
Referring to Figs. 17 to 18 there is illustrated an injector device 81 of an alternative embodiment of the invention (which is similar to the injector of Figs. 1 to 16 and like parts are assigned the same reference numerals). The injector device 81 comprises a barrel 3, an outlet nozzle 4 and a plunger 6. Two incompatible components 9 and 10 are placed within the barrel 3 of the injector device 81. The two components such as an antimicrobial 10 and a seal 9 are separated from one another by a barrier/membrane. The antimicrobial 10 is placed in the barrel 3 and a seal 9 is placed in a receptacle 86 such as a bag which is defined by an outer membrane 87 which provides the barrier.
The receptacle 86 may comprise a capsule into which the seal is filled on manufacture. The capsule may then be readily dropped into the barrel 3 of the injector before a plunger 6 is inserted.
When the nozzle 4 of the device 81 is inserted into a teat canal 16 a user depresses the plunger 6 to effect delivery of the antimicrobial 10 from the injector device into the teat canal (Figs. 17(a)). After the antimicrobial 10 is expelled from the injector device further pressure applied to the plunger results in puncturing or bursting of the receptacle 86 allowing egress of the seal 9 which is delivered into the teat canal (Fig. 17(b)). Fig. 17(d) shows the position of a seal formulation 9 and antimicrobial formulation 8 on delivery into the teat of a non-human animal.

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The injector device may comprise rupturing means such as sharp teeth 88 at the distal end of the barrel 3 to assist in puncturing or bursting the receptacle 86.
It will be appreciated that for ease of manufacture and use, the receptacle 86 may be attached to the plunger 6. In this case the receptacle is unable to come into contact with the rupturing device until substantially all of the first component has been expelled from the device.
When delivering a seal 9 and antimicrobial or anti-inflammatory formulation 10 into the teat canal of a non-human animal the injector device is typically positioned vertically below the teat with the delivery nozzle uppermost. The seal formulation 9 has a much higher specific gravity than the antimicrobial or anti-inflammatory formulation 10 and therefore the receptacle containing the seal formulation remains at the lower end of the barrel 3 containing the antimicrobial or anti-inflammatory formulation during the delivery of the antimicrobial or anti-inflammatory formulation into the teat. In this case the receptacle comes into contact with the rupturing device only when substantially all of the antimicrobial or anti-inflammatory formulation has been delivered into the teat.
Referring to Figs. 19 and 20 there is illustrated another injector device 90 of the invention. The injector device 90 comprises an inner barrel 92 and an outer barrel 93. The outer barrel 93 has a nozzle 94. The inner barrel 92 comprises a breakable/burstable barrier or membrane at its distal end and a plunger 98 at its proximal end. Antimicrobial 10 is contained within the outer barrel 93. In use, the nozzle 94 is inserted into a teat canal 16. The inner barrel 92 is pushed through the outer barrel 93 to expel the antimicrobial (Fig. 20(b)). This procedure may be facilitated by a flange 97 around the proximal end of the inner barrel 92 and a flange 99 around the proximal end of the outer barrel 93. When the antimicrobial 10 has been expelled (Fig. 20(c)) the plunger 98 on the inner barrel 92 is depressed to expel the seal 9 from the inner barrel 91. The pressure of the plunger 98 is sufficient to

WO 2005/072644 PCT/IE2005/000007
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burst or rupture the barrier/membrane 96 on the inner barrel 92 allowing the seal 9 to be expelled from the injector device through the nozzle 94 and into the teat canal (Fig. 20(c)). Alternatively rupturing means such as sharp teeth situated within the outer barrel 93 at its aperture into the nozzle, ruptures the barrier/membrane 96.
A seal 96 may be provided at the front end of the inner injector barrel 92 to provide a positive seal. The inner injector may be moulded with a weak burstable barrier/membrane 95 across the outlet aperture.
It will be appreciated that the seal portion of the formulation may be manufactured in one facility and subsequently combined with the anti-bacterial portion of the formulation at a later stage, in the same or another facility.
The invention is not limited to the embodiments hereinbefore described which may be varied in detail.

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Claims
1. Use of a single delivery device for treating or preventing or suppressing a
disease or condition in a non-human animal comprising the steps of: -
providing a single delivery device containing two components for sequential delivery from the delivery device;
delivering a first component from the single delivery device into a teat canal of a non-human animal; and
subsequently delivering the second component from the single delivery device into the teat canal without substantial mixing of the components.
2. Use as Iclaimed in claim 1 wherein the delivery device comprises an injector
device containing the two components, the components being separated by a
barrier and the method comprises the steps of: -
delivering the first component from the injector device; at least partially releasing the barrier; and
subsequently delivering the second component from the injector device.
3. Use as claimed in claim 1 or 2 wherein the disease or condition is mastitis.
4. Use as claimed in claim 1 or 2 for treating or preventing a mastitis-causing
micro-organism.

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5. Use as claimed in any of claims 1 to 4 wherein the second component
comprises a seal.
6. Use of a single injector device for treating preventing or suppressing mastitis
or a mastitis causing micro-organism comprising the steps of sequentially
delivering from a single delivery device an antimicrobial formulation and a
seal formulation into the teat canal of a non-human animal wherein the
antimicrobial formulation and the seal formulation are delivered into the teat
canal without substantial mixing of the formulations prior to delivery into the
teat canal.
7. Use as claimed in claim 5 or 6 wherein the seal formulation comprises a non-
toxic heavy metal salt.
8. Use as claimed in claim 7 wherein the seal formulation comprises greater
than 40% by weight of the heavy metal salt.
9. Use as claimed in claim 7 or 8 wherein the seal formulation comprises
between 50% and 75% by weight of the heavy metal salt.
10. Use as claimed in claims 7 to 9 wherein the seal formulation comprises
approximately 65% by weight of the heavy metal salt.
11. Use as claimed in claims 7 to 10 wherein the heavy metal is bismuth.
12. Use as claimed in claims 7 to 11 wherein the salt is a sub-nitrate salt.
13. Use as claimed in claims 7 to 12 wherein the seal formulation comprises a gel
base.

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14. Use as claimed in claim 13 wherein the gel base is a gel based on aluminium
stearate.
15. Use as claimed in claim 13 or 14 wherein the gel base includes liquid paraffin
as a vehicle.
16. Use as claimed in claims 1 to 5 or 7 to 15 wherein the first component
comprises an antimicrobial.
17. Use as claimed in claim 6 or 16 wherein the antimicrobial is selected from
any one or more of betalactam antibiotics, polymyxins, glycopeptides,
aminoglycosides, lincosamides, macrolides, pleuromutilins, "fenicols" such
as chloramphenicol and florfenicol, tetracylcines, sulphonamides and
potentiated sulphonamides such as mixtures of trimethoprim and one or more
sulphonamide, quinolones and fluoroquinolones, ionophores, courmarins
such as novobiocin, natural or synthetic peptides, aminoglycosides,
antimicrobial peptides or antimicrobials, lantibiotics, or other products of
bacteria and other micro-organisms.
18. Use as claimed in claim 17 wherein the betalactam is selected from any one
or more of penicillin, modified penicillin such as cloxacillin, amoxycillin,
ampicillin, cephalosporins or beta lactam antibiotics potentiated by beta
lactamase inhibitors such as clavulanic acid.
19. Use as claimed in claim 17 wherein the aminoglycoside is selected from any
one or more of streptomycin, dihydrostreptomycin, neomycin, gentamycin,
framycetin, aparamycin or kanamycin.
20. Use as claim 6 or 16 wherein the antimicrobial is selected from any one or
more of macrolide, lincosamide or pleuromutilin, erythromycin, spiramycin,

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tylosin, spiramycin, tilmicosin, lincomycin, spectinomysin, pirlimycin or tiamulin.
21. Use as claimed in claim 6 or 16 wherein the antimicrobial is selected from
any one or more of potentiated sulphonamide mixtures, trimethoprim plus
sulphadiazine, sulphadimidine, sulphadoxine, sulphadimethoxine or other
sulphonamide, oxytetracycline, minocycline or doxyclycline,
fiuoroquinolones, enrofloxacin, ciprofloxacin, norfloxacin, danofioxacin,
difloxacin or marbofloxacin.
22. Use of claim 1 to 5 or 7 to 15 wherein the first component comprises an anti-
inflammatory.
23. Use as claimed in claim 22 wherein the anti-inflammatory is selected from
any one or more of steroids such as prednisolone, betamethazone,
dexamethazone, phenylbutazone, or non-steroids such as flunixin,
ketoprofen, carprofen, vedaprofen, meloxicam, tepoxalin, eltenac, nimesulide
or tolfenamic acid.
24. Use substantially as hereinbefore described with reference to the drawings.
25. An injector device for delivery of components into the teat canal of a non-
human animal comprising:
a barrel for containing a first component,
an outlet nozzle at one end of the barrel,
an internal receptacle for containing a second component,
a barrier for separating a first component and a second component, and

WO 2005/072644 PCT/1E20O5/0OO007
27
a delivery means for delivery of a first component from the barrel and sequential delivery of a second component from the internal receptacle through the outlet nozzle.
26. An injector device as claimed in claim 25 wherein the barrier is normally
closed.
27. An injector device as claimed in claims 25 or 26 wherein the barrier is
releasable for delivery of the second component.
28. An injector device as claimed in claims 25 to 27 wherein the barrier is defined
by at least a portion of the internal receptacle.
29. An injector-device as claimed in claims 25 to 28 wherein the barrier comprises
one or more passageways.
30. An injector device as claimed in claim 29 wherein the one or more
passageways are opened when the barrier is released for delivery of the second
component through said one or more passageways.
31. An injector device as claimed in claims 25 to 30 wherein the device comprises
an activator, for releasing the barrier.
32. An injector as claimed in claim 31 wherein the activator comprises a
mechanical activator means.
33. An injector as claimed in claims 31 or 32 wherein the activator comprises at
least one projecting member.

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34. An injector as claimed in any of claims 31 to 33 wherein the activator is
located in the barrel.
35. An injector as claimed in any of claims 31 to 34 wherein the activator is
located adjacent to the outlet nozzle.
36. An injector device as claimed in claims 31 to 35 wherein the activator
comprises one or more passageways.
37. An injector device as claimed in claims 31 to 36 wherein the activator is
configured for engagement with the internal receptacle to provide a direct
passageway for delivery of the second component from the internal receptacle
into the outlet nozzle.
38. An injector; as claimed in any of claims 25 to 37 wherein the delivery means
comprises a plunger for the barrel.
39. An injector: device as claimed in any of claims 25 to 38 wherein the barrier is
released by ;the delivery means.
40. An injectorsas claimed in any of claims 25 to 39 wherein the internal receptacle
comprises an inner barrel located within an outer barrel defined by the barrel of
the injector.
41. An injector as claimed in claim 40 wherein the inner barrel is a close fit within
the outer barrel.
42. An injector as claimed in any of claims 40 or 41 wherein the delivery means
comprises the inner barrel.

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43. An injector as claimed in claims 40 to 42 wherein the inner barrel defines a
plunger for the outer barrel.
44. An injector as claimed in any of claims 40 to 43 wherein the delivery means
comprises a plunger for the inner barrel.
45. An injector as claimed in claims 40 to 44 wherein the inner barrel comprises
engagement means for engagement with the outer barrel on assembly.
46. An injector as claimed in claim 45 wherein the engagement means comprises
an external seal.
47. An injector as claimed in any of claims 45 or 46 wherein the outer barrel
comprises engagement means for engagement with the inner barrel.
48. An injector a's claimed in claim 47 wherein the outer barrel comprises a
locking ring for engagement with the inner barrel.
49. An injector as claimed in any of claims 40 to 48 wherein the inner barrel
comprises engagement means for engagement with the plunger.
50. An injector as claimed in claim 49 wherein the inner barrel comprises a
locking ring for engagement with the plunger.
51. An injector device as claimed in any of claims 25 to 39, wherein the receptacle
comprises a bag.
52. An injector device as claimed in any of claims 25 to 39, wherein the receptacle
comprises a capsule.

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53. An injector as claimed in any of claims 51 or 52 wherein the receptacle is
attached to or forms an integral part of the delivery means.
54. An injector device as claimed in any of claims 31 to 53 wherein the activator
comprises a rupturing means for at least partially releasing the barrier.
55. An injector as claimed in claim 54 wherein the rupturing means comprises a
mechanical rupture member.
56. An injector as claimed in claim 55 wherein the rupture member comprises at
least one blade.
57. An injector as claimed in claims 55 or 56 wherein the rupture member
comprises at least one tooth.
58. An injector! as claimed in any of claims 55 to 57 wherein the rupture member is
located in the barrel.
59. An injector as claimed in any of claims 55 to 58 wherein the rupture member is
located adjacent to the outlet nozzle.
60. An injector device as claimed in any of claims 25 to 59 wherein the barrel
contains a first component.
61. An injector device as claimed in claim 60 wherein the first component
comprises an antimicrobial formulation.
62. An injector device as claimed in any of claims 25 to 61 wherein the internal
receptacle contains a second component.

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31
63. An injector device as claimed in claim 62 the second component comprises a
seal formulation.
64. An injector device as claimed in any of claims 25 to 63 wherein a first
component is delivered from the barrel and a second component is
subsequently delivered from the internal receptacle without substantial mixing
of the components.
65. An injector device as substantially hereinbefore described with reference to the
accompanying drawings.
66. A method for treating or preventing or suppressing a disease or condition in a
non-human animal comprising the steps of: -
providing a single delivery device containing two components for sequential delivery from the delivery device;
delivering a first component from the single delivery device into a teat canal of anon-human animal; and
subsequently delivering the second component from the single delivery device into the teat canal without substantial mixing of the components.
67. A method as claimed in claim 66 wherein the delivery device comprises an
injector device containing the two components, the components being
separated by a barrier and the method comprises the steps of: -
delivering the first component from the injector device; at least partially releasing the barrier; and

WO 2005/072644 PCT/IE2005/000007
32
subsequently delivering the second component from the injector device.
68. A method as claimed in claim 66 or 67 wherein the disease or condition is
mastitis.
69. A method as claimed in claim 66 or 67 for treating or preventing a mastitis-
causing?micro-organism.
70. A method as claimed in any of claims 66 to 69 wherein the second
component comprises a seal.
71. A method for treating, preventing or suppressing mastitis or a mastitis
causing micro-organism comprising the steps of sequentially delivering from
a single delivery device an antimicrobial formulation and a seal formulation
into the teat canal of a non-human animal wherein the antimicrobial
formulation and the seal formulation are delivered into the teat canal without
substantial mixing of the formulations prior to delivery into the teat canal.
72. A method as claimed in claim 70 or 71 wherein the seal formulation
comprises a non-toxic heavy metal salt.
73. A method as claimed in claim 72 wherein the seal formulation comprises
greater than 40% by weight of the heavy metal salt.
74. A method as claimed in claim 72 or 73 wherein the seal formulation
comprises between 50% and 75% by weight of the heavy metal salt.
75. A method as claimed in any of claims 72 to 74 wherein the seal formulation
comprises approximately 65% by weight of the heavy metal salt.

WO 2005/072644 PCT/IE2005/000007
33
76. A method as claimed in any of claims 72 to 75 wherein the heavy metal is
bismuth.
77. A method as claimed in any of claims 72 to 75 wherein the salt is a sub-
nitrate salt.
78. A method as claimed in any of claims 70 to 77 wherein the seal formulation
comprises a gel base.
79. A method as claimed in claim 78 wherein the gel base is a gel based on
aluminium stearate.
80. A method as claimed in claim 78 or 79 wherein the gel base includes liquid
paraffin as a vehicle.
81. A method as claimed in any of claims 66 to 70 or 72 to 80 wherein the first
component comprises an antimicrobial.
82. A method as claimed in claim 71 or 81 wherein the antimicrobial is selected
from any one or more of betalactam antibiotics, polymyxins, glycopeptides,
aminoglycosides, lincosamides, macrolides, pleuromutilins, "fenicols" such
as chloraniphenicol and florfenicol, tetracylcines, sulphonamides and
potentiated sulphonamides such as mixtures of trimethoprim and one or more
sulphonamide, quinolones and fluoroquinolones, ionophores, courmarins
such as novobiocin, natural or synthetic peptides, aminoglycosides,
antimicrobial peptides or antimicrobials, lantibiotics, or other products of
bacteria and other micro-organisms.
83. A method as claimed in claim 82 wherein the betalactam is selected from any
one or more of penicillin, modified penicillin such as cloxacillin, amoxycillin,

WO 2005/072644 PCT/IE2005/000007
34
ampicillin., cephalosporins or beta lactam antibiotics potentiated by beta lactamase inhibitors such as clavulanic acid.
84. A method as claimed in claim 82 wherein the aminoglycoside is selected
from any one or more of streptomycin, dihydrostreptomycin, neomycin,
gentamycin, framycetin, aparamycin or kanamycin.
85. A method as claimed in claim 71 or 81 wherein the antimicrobial is selected
from any one or more of macrolide, lincosamide or pleuromutilin,
erythromycin, spiramycin, tylosin, spiramycin, tilmicosin, lincomycin,
spectinomysin, pirlimycin or tiamulin.
86. A method as claimed in claim 71 or 81 wherein the antimicrobial is selected
from any one or more of potentiated sulphonamide mixtures, trimethoprim
plus sulphadiazine, sulphadimidine, sulphadoxine, sulphadimethoxine or
other sulphonamide, oxytetracycline, minocycline or doxyclycline,
fluoroquinolones, enrofloxacin, ciprofloxacin, norfloxacin, danofloxacin,
difloxacin or marbofloxacin.
87. A method as claimed in any of claims 66 to 70 to 72 to 80 wherein the first
component comprises an anti-inflammatory.
88. A method as claimed in claim 87 wherein the anti-inflammatory is selected
from any one or more of steroids such as prednisolone, betamethazone,
dexamethazone, phenylbutazone, or non-steroids such as flunixin, ketoprofen,
carprofen, vedaprofen, meloxicam, tepoxalin, eltenac, nimesulide or
tolfenamic acid.
89. A method substantially as hereinbefore described with reference to the
accompanying drawings.
The invention relates to a method and device for treating or preventing or suppressing a disease or condition in a non-human animal. The method comprises the steps of providing a single delivery device containing two components for sequential delivery from the delivery device. A first component is delivered from the single delivery device into a teat canal of a non-human animal and subsequently the second component is delivered from the single delivery device into the teat canal. The components are delivered without substantial mixing of the components.

Documents:

02188-kolnp-2006 abstract.jpg

02188-kolnp-2006 abstract.pdf

02188-kolnp-2006 claims.pdf

02188-kolnp-2006 correspondence others.pdf

02188-kolnp-2006 description(complete).pdf

02188-kolnp-2006 drawings.pdf

02188-kolnp-2006 form-1.pdf

02188-kolnp-2006 form-3.pdf

02188-kolnp-2006 international publication.pdf

02188-kolnp-2006 international search authority report.pdf

02188-kolnp-2006 pct form.pdf

02188-kolnp-2006 priority document.pdf

02188-kolnp-2006-correspondence others-1.1.pdf

02188-kolnp-2006-g.p.a.pdf

2188-KOLNP-2006-(15-01-2013)-ABSTRACT.pdf

2188-KOLNP-2006-(15-01-2013)-CORRESPONDENCE.pdf

2188-KOLNP-2006-(20-12-2012)-ABSTRACT.pdf

2188-KOLNP-2006-(20-12-2012)-ANNEXURE TO FORM 3.pdf

2188-KOLNP-2006-(20-12-2012)-CLAIMS.pdf

2188-KOLNP-2006-(20-12-2012)-CORRESPONDENCE.pdf

2188-KOLNP-2006-(20-12-2012)-DESCRIPTION (COMPLETE).pdf

2188-KOLNP-2006-(20-12-2012)-DRAWINGS.pdf

2188-KOLNP-2006-(20-12-2012)-FORM-1.pdf

2188-KOLNP-2006-(20-12-2012)-FORM-13.pdf

2188-KOLNP-2006-(20-12-2012)-FORM-2.pdf

2188-KOLNP-2006-(20-12-2012)-OTHERS.pdf

2188-KOLNP-2006-(20-12-2012)-PETITION UNDER RULE 137.pdf

2188-KOLNP-2006-(23-08-2013)-ANNEXURE TO FORM 3.pdf

2188-KOLNP-2006-(23-08-2013)-CORRESPONDENCE.pdf

2188-KOLNP-2006-(23-08-2013)-DESCRIPTION (COMPLETE).pdf

2188-KOLNP-2006-(23-08-2013)-DRAWINGS.pdf

2188-KOLNP-2006-(23-08-2013)-FORM-1.pdf

2188-KOLNP-2006-(23-08-2013)-FORM-2.pdf

2188-KOLNP-2006-(23-08-2013)-OTHERS.pdf

abstract-02188-kolnp-2006.jpg

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Patent Number

260323

Indian Patent Application Number

2188/KOLNP/2006

PG Journal Number

17/2014

Publication Date

25-Apr-2014

Grant Date

23-Apr-2014

Date of Filing

02-Aug-2006

Name of Patentee

BIMEDA RESEARCH & DEVELOPMENT LIMITED

Applicant Address

BROOMHILL ROAD, TALLAGHT,DUBLIN 24

Inventors:

#	Inventor's Name	Inventor's Address
1	HALLAHAN STEPHEN	BIMEDA RESEARCH AND DEVELOPMENT LIMITED, BROOMHILL ROAD, TALLAGHT, DUBLIN-24.
2	SMITH BRENDAN	146 VERNON AVENUE, CLONTRAF, DUBLIN 3
3	VAN VESSEM LOUIS	MARTIN LUTHER KINGLAAN 139, NL-3223 GG, HELLEVOETSLUIS
4	MCHARDY NICHOLAS	BALTYBOYS, COUNTY WICKLOW

PCT International Classification Number

A61D 1/02

PCT International Application Number

PCT/IE2005/000007

PCT International Filing date

2005-02-02

PCT Conventions:

#	PCT Application Number	Date of Convention	Priority Country
1	60/540,336	2004-02-02	Ireland
2	2004/0364	2004-05-24	Ireland