Title of Invention	BENZOPHENONE LINKED PYRROLO [2,1-C][1,4]BENZODIAZEPINE HYBRIDS AND A PROCESS FOR THE PREPARATION THEREOF
Abstract	The present invention provides a compound of general formula 5, useful as potential antitumour agents against human cancer cell lines. The present invention further provides a process for the preparation of pyrrolo [2,1-c][1,4] benzodiazepine hybrids of general formula 5 wherein, X1, X2, X3 is selected from H or Cl or CH3, Y is selected from O or NH, Z is selected from C =O or CH2 and n=1 to 4.

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FIELD OF THE INVENTION (The present invention relates to Benzophenone linked pyrrolo[2,1-c][1,4] benzodiazepine hybrids and a process for the preparation thereof. More particularly it relates to 7-methoxy-8-{n-[2-benzoyl-(4-chlorophenyloxy)alkyl]oxy}-(11aS)-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4] benzodiazepine-5-one with aliphatic chain length variations useful as anticancer (antitumour) agent. )The structural formula of these Benzophenone linked pyrrolo [2,1-c][1,4] benzodiazepines hybrids is given below. (Figure Removed) BACKGROUND OF THE INVENTION Pyrrolo [2,1-c][1,4]benzodiazepine antitumour antibiotics are commonly known as anthramycin class of compounds. In the last few years, a growing interest has been shown in the development of new pyrrolo [2,1-c][1,4]benzodiazepines (PBDs). These antibiotics react covalently with DNA to form an N2-guanine adduct that lies within the minor groove of duplex DNA via an acid-labile aminal bond to the electrophilic imine at the N10-C11 position (Kunimoto, S.; Masuda, T.; Kanbayashi, N.; Hamada, M.; Naganawa, H.; Miyamoto, M.; Takeuchi, T.; Unezawa, H. J. Antibiot, 1980, 33, 665.; Kohn, K. W. and Speous, C. L. J. Mol. Biol., 1970, 51, 551.; Hurley, L. H.; Gairpla, C. and Zmijewski, M. Biochem. Biophys. Acta., 1977, 475, 521.; Kaplan, D. J. and Hurley, L. H. Biochemistry, 1981, 20, 7572). The molecules have a right-handed twist, which allows them to follow the curvature of the minor groove of B-form double-stranded DNA spanning three base pairs. A recent development has been the linking of two PBD units through their C-8 positions to give bisfunctional-alkylating agents capable of cross-linking DNA (Thurston, D. E.; Bose, D. S.; Thomson, A. S.; Howard, P. W.; Leoni, A.; Croker, S. J.; Jenkins, T. C.; Neidle, S. and Hurley, L. H. J. Org. Chem. 1996, 61, 8141). (Figure Removed) Anthramycin dimers; n = 3-5 Recently, PBD dimers have been developed that comprise of two C2-exo-methylene substituted DC-81 subunits tethered through their C-8 position via an inert propanedioxy linker (Gregson, S. J.; Howard, P. W.; Hartely, J. A.; Brooks, N. A.; Adams, L. J.; Jenkins, T. C.; Kelland, L. R. and Thurston, D. E. J. Med. Chem. 2001, 44, 737). A non-cross-linking mixed imine-amide PBD dimers have been synthesized that have significant DMA binding ability and potent antitumour activity (Kama), A.; Ramesh, G. Laxman, N.; Ramulu, P.; Srinivas, 0.; Neelima, K.; Kondapi, A. K.; Srinu, V. B.; Nagarajaram, H. M. J. Med. Chem. 2002, 45, 4679). Recently, some new pyrrolobenzodiazepine (PBD) hybrids have been synthesized that have significant DNA binding ability and potent antitumour activity.( Kamal, A.; Srinivas, O.; Ramulu, P.; Ramesh, G.; Kumar, P. P. Bioorg. Med. Chem. Lett. 2003, 13, 3577). Naturally occurring pyrrolo [2,1-c][1,4]benzodiazepines belong to a group of antitumour antibiotics derived from Streptomyces species. Recently, there is much impetus for the PBD systems as they can recognize and bind to specific sequence of DNA. Examples of naturally occurring PBDs include anthramycin, DC-81, tomaymycin, sibiromycin and neothramycin. However, the clinical efficacy for these antibiotics is hindered by several limitations, such as poor water solubility, cardio toxicity, development of drug resistance and metabolic inactivation. OBJECTIVES OF THE INVENTION The main objective of the present invention is to provide Novel Benzophenone pyrrolo [2,1-c] [1,4]benzodiazepine hybrids, useful as antitumour agents. Yet another object of this invention is to provide a process for the preparation of Novel Benzophenone pyrrolo [2,1-c] [1,4]benzodiazepine hybrids. SUMMARY OF THE INVENTION Accordingly the present invention provides a novel benzophenone linked pyrrolo [2,1-c][1,4] benzodiazepine hybrid of general formula 5 (Formula Removed) In an embodiment of the present invention the novel benzophenone linked pyrrolo [2,1-c][1,4] benzodiazepine hybrid as claimed in claim 1 is represented by the group of the following compounds: 7-methoxy-8-{3-[2-benzoyl-(4-chlorophenyloxy)propoxy]}-(11aS)-1,2,3,11a-tetra hydro-5H-pyrrolo[2,1-c][1,4] benzodiazepine-5-one (5a); 7-methoxy-8-{4-[2-benzoyl-(4-chlorophenyloxy)butoxy]}-(11aS)-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4] benzodiazepine-5-one (5b);7-methoxy-8-{5-[2-benzoyl-(4chlorophenyloxy)pentoxy]}-(11aS)-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepine-5-one(5c);7-met hoxy-8-{3-[2-benzoyl-(4-chloro-6-methylphenyloxy)propoxy]}-(11aS)-1,2,3,11a-tetra hydro-5H-pyrrolo[2,1-c][1,4] benzodiazepine-5-one (5d); 7-methoxy-8-{3-[2-benzoyl-(4-chloro-6-methylphenyloxy)butoxy]}-(11 aS)-1,2,3,11 a-tetrahydro-5H-pyrrolo[2,1 -c] [1,4]benzodiazepine-5-one(5e);7-methoxy-8-{3-[2-benzoyl-(4-chloro-6-methyl phenyloxy)pentoxy]}-(11aS)-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4] benzo diazepine-5-one (5f); 7-methoxy-8-{3-[2-benzoyl-(4,6-dichlorophenyloxy)propoxy]}-(11aS)-1,2,3,11a-tetra hydro-5H-pyrrolo[2,1-c][1,4] benzodiazepine-5-one (5g); 7-methoxy-8-{3-[2-benzoyl-(4,6-dichlorophenyloxy)butoxy]}-(11aS)-1,2,3,11a-tetra hydro-5H-pyrrolo[2,1-c][1,4] benzodiazepine-5-one (5h);7-methoxy-8-{3-[2-benzoyl-(4,6-di-chlorophenyloxy)hexyloxy]}-(11 aS)-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4] benzodiazepine-5-one (5i 7-Methoxy-8-{N1-[4-chloro-2-(2-chlorobenzoyl)phenyl]2-oxyacetamido}-(11aS)-1,2,3,11a-tetrahydro-5H -pyrrolo[2,1-c][1,4]benzodiazepine-5-one(5j);7-Methoxy-8-{N1-[4-chloro-2-(2-chlorobenzoyl)phenyl]2-oxypropinamido}-(11aS)-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzod iazepine-5-one5k);7-Methoxy-8-{N1-[4-chloro-2-(2-chlorobenzoyl)phenyl]2-oxybutanamido}-(11aS)-1,2,3,11 a-tetrahydro-5H-pyrrolo[2,1 -c][1,4]benzodiazepine-5-one (51). In yet another embodiment the structural formula of the representative compounds of benzophenone linked pyrrolo [2,1-c][1,4] benzodiazepine hybrid are: (Figure Removed) In yet another embodiment the novel benzophenone linked pyrrolo [2,1-c][1,4]benzodiazepine hybrid exhibits an in vitro anticancer/antitumour activity against human cancer cell lines selected from the group consisting of lung (Hop-62), cervix (SiHa), breast (MCF7, Zr-75-1), colon (Colo205), prostate (DU145, PC3) and oral (DWD, HT1080) cell lines. In yet another embodiment the concentration of benzophenone linked pyrrolo[2,1-c][1,4]benzodiazepine hybrid used for in vitro activity against Colo205 for IC50 is in the range of 17 to 80μm, at an exposure period of at least 48 hrs. In yet another embodiment the concentration of benzophenone linked pyrrolo[2,1-c][1,4]benzodiazepine hybrids used for in vitro activity against DU145 for IC50 is in the range of 16 to 80μm, at an exposure period of at least 48 hrs. In yet another embodiment the concentration of benzophenone linked pyrrolo[2,1-c][1,4]benzodiazepine hybrids used for in vitro activity against DWD for IC50 is in the range of 6 to 80μm, at an exposure period of at least 48 hrs. In yet another embodiment the concentration of benzophenone linked pyrrolo[2,1-c][1,4]benzodiazepine hybrids used for in vitro activity against HoP62 for IC50 is in the range of 13 to 40 μm, at an exposure period of at least 48 hrs. In yet another embodiment the concentration of benzophenone linked pyrrolo[2,1-c][1,4]benzodiazepine hybrids used for in vitro activity against HT1080 for IC50 is in the range of 6 to 30μrn, at an exposure period of at least 48 hrs. In yet another embodiment the concentration of benzophenone linked pyrrolo[2,1-c][1,4]benzodiazepine hybrids used for in vitro activity against MCF7 for IC50 is in the range of 27 to about 80μm, at an exposure period of at least 48 hrs. In yet another embodiment the concentration of benzophenone linked pyrrolo[2,1-c][1,4]benzodiazepine hybrids used for in vitro activity against PCS for IC50 is in the range of 9 to about 80μm, at an exposure period of at least 48 hrs. In yet another embodiment the concentration of benzophenone linked pyrrolo[2,1-c][1,4]benzodiazepine hybrids used for in vitro activity against SiHa for IC50 is in the range of 25 to about 80μm, at an exposure period of at least 48 hrs. In yet another embodiment the concentration of benzophenone linked pyrrolo[2,1-c][1,4]benzodiazepine hybrids used for in vitro activity against Zr-75-1 for IC50 is in the range of 24 to about 80μm, at an exposure period of at least 48 hrs. The present invention further provides a pharmaceutical composition comprising benzophenone linked pyrrolo[2,1-c][1,4] benzodiazepine hybrid, its derivatives, analogues, salts or mixture thereof optionally with pharmaceutically acceptable carriers, adjuvants and additives. In yet another embodiment the benzophenone linked pyrrolo[2,1 c][1,4]benzodiazepine hybrid used is represented by a general formula 5, (Formula Removed) wherein n = 1, 2, 3 or 4. The present invention further provides A process for the preparation of benzophenone linked pyrrolo[2,1-c][1,4]benzodiazepine hybrid of formula 5, (Formula Removed) wherein n = 1, 2, 3 or 4, the said process comprising the steps of: a) reacting(2S)-N-(4-hydroxy-3-methoxy-2-nitrobenzoyl)pyrrolidine-2-carbox aldehyde diethylthioacetal of formula 2 (Formula Removed) [2-(n-bromoalkyl)-n-chlorophenyl)(phenyl) methanone or acetamide of formula 1, (Formula Removed) wherein X1, X2 and X3 is selected from the group consisting of H, Cl and CH3; Y is selected from O and NH; Z is selected from C=O and CH2; n = 1to 4, in an aprotic water miscible organic solvent, in the presence of anhydrous mild inorganic base, under refluxing temperature in an oil bath, for a period of about 48 hrs, followed by the removal of inorganic base by filtration and evaporating the organic solvent to obtain the resultant crude product and purifying it by known method to obtain the desired product of (2S)-N-{n benzoyl (phenyloxy) alkoxy/ [(n benzoyl) phenyl]2-oxyacetamido] 5-methoxy-2-nitrobenzoyl}-pyrrolidine-2-carboxaldehyde diethylthioacetal of formula 3, (Formula Removed) wherein X1,X2andX3 is selected from the group consisting of H, Cl and CH3; Y is selected from O and NH; Z is selected from C=O and CH2; n = 1 to 4, b)reducing (2S)-N-{n benzoyl (phenyloxy)alkoxy / (n benzoyl) phenyl] 2 oxy acetamido]-5-methoxy-2-nitrobenzoyl}-pyrrolidine-2-carboxaldehydediethyl Ithioacetal of formula 3 with anhydrous tin chloride, in an alcohol, under reflux, followed by the evaporation of alcohol and adjusting the pH of the resultant product layer to about 8 by using a base, followed by extraction with ethyl acetate and washing the combined organic phase with brine solution and evaporating the solvent to obtain the desired (2S)-N-{n-benzoyl (phenyloxy)alkoxy / [(n-benzoyl) phenyl]2-oxyacetamido]-5-methoxy-2-amino benzoyl}-pyrrolidine-2-carboxaldehyde diethylthioacetal of formula 4, (Formula Removed) wherein X1, X2 and X3 is selected from the group consisting of H, Cl and CHs; Y is selected from O and NH; Z is selected from C=O and CH2; n = 1 to 4, b)reacting (2S)-N-{n benzoyl (phenyloxy)alkoxy / [ (n benzoyl) phenyl] 2-oxy acetamido]-5-methoxy-2-aminobenzoyl}-pyrrolidine-2-carboxaldehydediethyl thioacetal of formula 4 with mercurous chloride, in a mixture of water and organic solvent, in the presence of mild inorganic, under stirring, at a temperature of about 20-30°C, for a period of 8-12 hrs, followed by the extraction of yellow organic supernatant and washing with sodium bi carbonate and brine, respectively, and evaporating the organic layer, under reduced pressure to obtain the desired product of benzophenone linked pyrrolo[2,1-c][1,4] benzodiazepine _hybrid of formula 5. In yet anther embodiment the mild inorganic base used in steps (a) & (b) is calcium carbonate. In yet anther embodiment the aprotic organic solvent used in step (a) is acetone and acetonitrile In yet anther embodiment the organic solvent used in step (c) is acetonitrile and acetone In yet anther embodiment the alcohol used in step (b) is selected from methanol and ethanol. In yet anther embodiment the compounds of formula 5 obtained are represented by a group of the following compounds: 7-methoxy-8-{3-[2-benzoyl-(4-chlorophenyloxy)propoxy]}-(11 aS)-1,2,3,11a-tetra hydro-5H-pyrrolo[2,1-c][1,4]benzodiazepine-5-one(5a);7-methoxy-8-{4-[2-benzoyl- (4-chlorophenyloxy)butoxy]}-(11aS)-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4] benzodiazepine-5-one(5b);7-methoxy-8-{5-[2-benzoyl-(4chlorophenyloxy) pentoxy]}-(11aS)-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepine-5- one(5c);7-methoxy-8-{3-[2-benzoyl-(4-chloro-6-methylphenyloxy)propoxy]}-(11aS) -1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepine-5-one(5d);7-methoxy- 8-{3-[2-benzoyl-(4-chloro-6-methylphenyloxy)butoxy]}-(11aS)-1,2,3,11a-tetra hydro-5H-pyrrolo[2,1-c][1,4]benzodiazepine-5-one(5e);7-methoxy-8-{3-[2-benzoyl- (4-chloro-6-methylphenyloxy)pentoxy]}-(11aS)-1,2,3,11a-tetrahydro-5H-pyrrolo [2,1-c][1,4]benzodiazepine-5-one(5f);7-methoxy-8-{3-[2-benzoyl-(4,6-dichloro phenyloxy)propoxy]}-(11aS)-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4] benzo diazepine-5-one(5g); 7-methoxy-8-{3-[2-benzoyl-(4,6-dichlorophenyloxy) butoxy]}-(11aS)-1,2,3,11a-tetra hydro-5H-pyrrolo[2,1-c][1,4] benzodiazepine-5-one (5h); 7-methoxy-8-{3-[2-benzoyl-(4,6-di-chlorophenyloxy)hexyloxy]}-(11 aS)-1,2,3,11 a-tetrahydro-5/-/-pyrrolo[2,1-c][1,4] benzodiazepine-5-one (5i 7-Methoxy-8-{N1-[4-chloro-2-(2-chlorobenzoyl)phenyl]2-oxyacetamido}-(11aS)-1,2,3,11a-tetrahydro-5H -pyrrolo[2,1-c][1,4]benzodiazepine-5-one (5j); 7-Methoxy-8-{N1-[4-chloro-2-(2-chlorobenzoyl)phenyl]2-oxypropinamido}-(11aS)-1,2,3,11a-tetrahydro-5H-pyrrolo [2,1-c][1,4]benzodiazepine-5-one5k);7-Methoxy-8-{N1-[4-chloro-2-(2-chloro benzoyl)phenyl]2-oxybutanamido}-(11aS)-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepine-5-one (51). In still anther embodiment the benzophenone linked pyrrolo [2,1-c][1,4]benzodiazepine hybrid of formula 5a-m exhibits an in vitro anticancer/antitumour activity against human cancer cell lines selected from the group consisting of lung, cervix, breast, colon, prostate and oral cell lines. DETAILED DESCRIPTION OF THE INVENTION Accordingly, the present invention provides a process for preparation of pyrrolo [2,1-c][1,4] benzodiazepine hybrids of formula 5 of the drawing accompanying the specification where n = 1-5, which comprises reacting {2-[(n-bromoalkyl)-3,5 dichloro phenyl](phenyl) methanone of formula 1 with 2S-N-(4-hydroxy-5-methoxy-2-nitrobenzoyl) pyrrolidine-2-carboxaldehyde diethylthioacetal of formula 2 in presence of CH3COCH3/K2CO3 for a period of 48 h with isolating (2S)-N-{3-[benzoyl(4-chlorophenyloxy)propyl]oxy-5-methoxy-2-nitrobenzoyl}pyrrolidi-ne-2-carboxaldehyde diethylthioacetal of formula 3 by conventional methods, reducing the above nitro compound of formula 3 with SnCl2.2H2O in presence of organic solvent with reflux temperature, resulting with the formation of (2S)-N-{3-[benzoyl(4-chlorophenyloxy]propoxy]}-5-methoxy-2-aminobenzoyl} pyrrolidine-2-carboxaldehyde diethylthioacetal 4 respectively by known methods, reacting the above said amino compound of formula 4 with known deprotecting agents in a conventional manner to give novel pyrrolo[2,1-c][1,4]benzodiazepine hybrids of formula 5, where 'n' is as stated above. The precursors, [2-(n-bromoalkyl)-n-chlorophenyl)(phenyl)methanone of formula 1 (Liou, J. P.; Chang, C.W.; Song, J. S.; Yang, Y. N.; Yeh, C. F.; Tseng, H. Y.; Lo, Y. K.; Chang, Y. L; Chang, C. M.; Hsieh, H. P.; J. Med. Chem. 2002, 45, 2556-2562). and(2S)-N-(4-hydroxy-5-methoxy-2-nitrobenzoyl)pyrrolidine-2-carboxaldehyde diethyl-thioacetal of formula 2 (Thurston, D. E.; Morris, S. J.; Hartley, J. A. Chem. Commun. 1996, 563-565) have been prepared by literature methods. Some representative compounds of formula 5 for the present inventions are given below a) 7-Methoxy-8-{3-[2-benzoyl-(4-chlorophenyloxy)propoxy]}-(11aS)-1,2,3,11a- tetrahydro-5H-pyrrolo[2,1-c][1,4] benzodiazepine-5-one. b) 7-Methoxy-8-{4-[2-benzoyl-(4-chlorophenyloxy)butoxy]}-(11 aS)-1,2,3,11 a- tetrahydro-5H-pyrrolo[2,1-c][1,4] benzodiazepine-5-one. c) 7-Methoxy-8-{3-[2-benzoyl-(4,6-dichlorophenyloxy)propoxy]}-(11aS)-1,2,3,11a- tetrahydro-5H -pyrrolo[2,1 -c][1,4] benzodiazepine-5-one d) 7-Methoxy-8-{3-[2-benzoyl-(4,6-dichlorophenyloxy)hexyloxy]}-(11 aS)- 1,2,3,11 a-tetrahydro-5H -pyrrolo[2,1 -c][1,4] benzodiazepine-5-one e) 7-Methoxy-8-{N 1 -[4-chloro-2-(2-chlorobenzoyl)phenyl]2-oxyacetamido}- (11aS)-1,2,3,11a-tetrahydro-5H -pyrrolo[2,1-c][1,4]benzodiazepine-5-one. The process for the preparation of new pyrrolo [2,1-c][1,4] benzodiazepine hybrids is disclosed and claimed in our copending copatent application no. These new analogues of pyrrolo [2,1-c][1,4] benzodiazepine hybrids linked at C-8 position have shown promising DNA binding activity and efficient anticancer activity in various cell lines. The molecules synthesized are of immense biological significance with potential sequence selective DNA-binding property. This resulted in design and synthesis of new congeners as illustrated in Scheme-1, which comprise: 1. The ether linkage at C-8 position of DC-81 intermediates with [2-(n- bromoalkyl)-5-chloropheny](phenyl) methanone moiety. 2. Refluxing the reaction mixtures for 48 h. 3. Synthesis of C-8 linked PBD antitumour antibiotic hybrid imines. 4. Purification by column chromatography using different solvents like ethyl acetate, hexane, dichloromethane and methanol. The following examples are given by way of illustration and therefore should not be construed to the present limit of the scope of invention. Example 1 To a solution of (2S)-N-(4-hydroxy-3-methoxy-2-nitrobenzoyl)pyrrolidine-2-carboxaldehyde diethylthioacetal 2 £500 mg, 1.25 mmol) in dry acetone (20 mL) was added anhydrous potassium carbonate (862 mg, 6.25 mmol) and [2-(3-bromopropyl)-5-chlorophenyl](phenyl)methanone 1 (441 mg, 1.25 mmol). The reaction mixture was refluxed for 48 h and the reaction was monitored by TLC using ethyl acetate-hexane (4:6) as a solvent system. The potassium carbonate was then removed by suction filtration and the solvent was evaporated under vacuum to afford the crude product. This was further purified by column chromatography using ethyl acetate: hexane (3:7) as a solvent system to obtain the pure product 3 (672 mg, 80% yield). 1H NMR (CDCI3) 81.16-1.34 m, 6H), 1.58-2.14 (m, 6H), 2.28-2.41 (m, 3H), 2.60-2.93 (m, 4H), 3.14-3.28 (m, 2H), 3.98 (s, 3H), 4.17-4.21 (t, 2H), 4.54-4.66 (m, 1H), 6.78 (s, 1 H), 6.85-6.95 (d, J = 4.87 1H), 7.30-7.60 (m, 7H), 7.65-7.80 (d, 1H); FABMS: 672 (M+H); (2S)-A/-{3-[Benzoyl(4-chlorophenyloxy)propoxy]}-5-methoxy-2-nitrobenzoyl}-pyrrolidine-2-carboxaldehyde diethylthioacetal of formula 3 (500 mg, 0.74 mmol) was dissolved in methanol (10 mL), SnCI2.2H2O (839 mg, 3.7 mmol) was added and refluxed until the TLC indicated the completion of the reaction. The methanol was evaporated by vacuum and the aqueous layer was then adjusted to pH 8 with 10% NaHCO3 solution and extracted with ethyl acetate (2x30 mL). The combined organic phase was dried over Na2SO4 and evaporated under vacuum to afford the crude amino diethylthioacetal 4 (450 mg, 95% yield), which was directly used in the next step. A solution of (2S)-N- {3-[benzoyl(4-chlorophenyloxy)propoxy]}-5-methoxy-2-amino-benzoyl}pyrrolidine-2-carboxaldehyde diethylthioacetal 4 (400 mg.0.62 mmol), HgCl2 (372 mg, 1.37 mmol) and CaC03 (157 mg, 1.55 mmol) in acetonitrile-water (4:1) was stirred slowly at room temperature for overnight until complete loss of starting material as indicated by the TLC. The clear organic supernatant liquid was extracted with ethyl acetate and washed with saturated 5% NaHCO3 (20 ml), brine (20 mL) and the combined organic phase was dried over Na2SO4. The organic layer was evaporated in vacuum to afford a white solid, which was first eluted on a column chromatography with ethyl acetate to remove mercuric salts, and then with ethyl acetate to obtain the pure product 5a (195 mg, 60% yield). 1H NMR (CDCI3) § 1.90-2.12 (m, 2H), 2.22-2.46 (m, 3H), 3.50-3.88 (m, 3H), 3.95 (s, 3 H), 4.24-4.35 (m, 4H), 6.54 (s, 1H), 6.88-6.96 (m, 1H), 7.24-7.26 (s, 1H), 7.30-7.45 (m, 3 H), 7.46-7.52 (m, 2H), 7.66-7.78 (m, 2H); FABMS: 524 (M+H) Example 2 To a solution of (2S)-N-(4-hydroxy-3-methoxy-2-nitrobenzoyl)pyrrolidine-2-carboxaldehyde diethylthioacetal 2 (512 mg, 1.28 mmol) in dry acetone (20 ml) was added anhydrous potassium carbonate (883 mg, 6.40 mmol) and [2-(4-bromobutoxy)-5-chlorophenyl](phenyl)methanone 1 (469 mg, 1.28 mmol). The reaction mixture was refluxed for 48 h and the reaction was monitored by TLC using ethyl acetate-hexane (2:8) as a solvent system. The potassium carbonate was then removed by suction filtration and the solvent was evaporated under vacuum to afford the crude product. This was further purified by column chromatography using ethyl acetate: hexane (3:7) as a solvent system to obtain the pure product 3 (721 mg, 82% yield). 1H NMR (CDCI3) 81.18-1.38(171, 8H), 1.59-2.18 (m, 6H), 2.26-2.42 (m, 3H), 2.53-2.90 (m, 4H), 3.18-3.29 (m, 2H), 3.96 (s, 3H), 4.18 (t, 2H), 4.54-4.55 (m, 1H), 6.73 (s, 1H), 7.4 (s, 1H), 7.6-7.68 (m, 3H), 7.75-7.85 (m, 3H), 7.98-8.0 (m, 2H); FABMS: 687(M+H) 2S)-N-{3-[Benzoyl(4-chlorophenyloxy)butoxy]}-5-methoxy-2-nitrobenzoyl} pyrrolidine- 2-carboxaldehyde diethylthioacetal of formula 3 (600 mg, 0.87 mmol) was dissolved in methanol (10 mL), SnCI2.2H2O (982 mg, 4.3 mmol) was added and refluxed until the completion of the reaction which was monitored by TLC. The methanol was evaporated by vacuum and the aqueous layer was then adjusted to pH 8 with 10% NaHCO3 solution and extracted with ethyl acetate (2x30 ml). The combined organic phase was dried over Na2SO4 and evaporated under vacuum to afford the crude amino diethylthioacetal 4 (545 mg, 95% yield), which was directly used in the next step. A solution of 2S)-N- {3-[benzoyl(4-chlorophenyloxy)butoxy]}-5-methoxy-2-aminoben- zoyl} pyrrolidine-2-carboxaldehyde diethylthioacetal 4 (500 mg, 0.76 mmol), HgCI2 (613 mg, 1.82 mmol) and CaCO3 (191 mg, 1.90 mmol) in acetonitrile-water (4:1) was stirred slowly at room temperature for overnight until complete consumption of starting material as indicated by the TLC. The clear organic supernatant liquid was extracted with saturated 5% NaHCO3 (20 mL), brine (20 ml) and the combined organic phase was dried over Na2SO4. The organic layer was evaporated in vacuum to afford a white solid, which was first eluted on a column chromatography with ethyl acetate to remove mercuric salts, and then with ethyl acetate to obtain the pure product 5b (225 mg, 55% yield). 1H NMR (CDCI3) 81.80-2.25 (m, 8H), 2.26-2.4 (m, 4H), 3.6 (s, 3H), 3.80-3.98 (m, 1H), 4.0-4.3 (m, 4H), 6.54 (s, 1H), 6.88-6.96 (m, 1H), 7.24-7.26 (s, 1H), 7.30-7.45 (m, 3H), 7.46-7.52 (m, 2H), 7.66-7.78 (m, 2H); FABMS: 538 (M+H) Example 3 To a solution of (2S)-N-(4-hydroxy)-3-methoxy-2-nitrobenzoyl)pyrrolidine-2-carboxaldehyde diethylthioacetal 2 (400 mg, 1 mmol) in dry acetone (20 mL) was added anhydrous potassium carbonate (686 mg, 5 mmol) and [2-(-bromobutoxy)-3,5-dichloro phenyl] (phenyl) methanone 1 (402 mg, 1 mmol). The reaction mixture was refluxed in an oil bath for 48 h and the reaction was monitored by TLC using ethyl acetate-hexane (4:6) as a solvent system. The potassium carbonate was then removed by suction filtration and the solvent was evaporated under vacuum to afford the crude product. This was further purified by column chromatography using ethyl acetate: hexane (3:7) as a solvent system to obtain the pure product 3 (578 mg, 82% yield). 1H NMR (CDCI3) 61.18-1.38(171, 8H), 1.59-2.18 (m, 6H), 2.26-2.42 (m, 3H), 2.53-2.90 (m, 4H), 3.18-3.29 (m, 2H), 3.96 (s, 3H), 4.18 (t, 2H), 4.54-4.55 (m, 1H), 6.73 (s, 1H), 7.4 (s, 1H), 7.6-7.68 (m, 3H), 7.75-7.85 (m, 3H), 7.98-8.0 (m, 2H); FABMS: 706 (M+H) 2S)-N-{3-[2-Benzoyl(4,6-dichlorophenyloxy)butoxy]}-5-methoxy-2-nitro benzoyl}pyrrolidine-2-carboxaldehyde diethylthioacetal of formula 3 (500 mg, 0.70 mmol) was dissolved in methanol (10 ml), SnCl2.2H2O (796 mg, 3.54 mmol) was added and refluxed until the TLC indicated the completion of the reaction. The methanol was then evaporated in vacuum and the aqueous layer was then adjusted to pH 8 with 10% NaHCO3 solution and extracted with ethyl acetate (60 mL). The combined organic phase was dried over Na2SO4 and evaporated under vacuum to afford the crude amino diethyl thioacetal 4 (470 mg, 96% yield), which was directly used in the next step. A solution of 2S)-N-{3-[2-benzoyl(4,6-dichloro phenyloxy)butoxy]}-5-methoxy-2-aminobenzoyl} pyrrolidine-2-carboxaldehyde diethylthioacetal of formula 4 (400 mg, 0.57 mmol), HgCI2 (377 mg, 1.3 mmol) and CaCO3 (146 mg, 1.44 mmol) in acetonitrile-water (4:1) was stirred slowly at room temperature overnight until complete consumption of starting material as indicated by the TLC. The clear organic supernatant liquid was extracted with ethyl acetate and washed with saturated 5% NaHCO3 (20 ml), brine (20 mL) and the combined organic phase was dried over Na2SO4. The organic layer was evaporated in vacuum to afford a white solid, which was first eluted on a column chromatography with ethyl acetate to remove mercuric salts, and then with ethyl acetate to obtain the pure product 5c (183 mg, 56% yield). 1H NMR (CDCI3) 81.80-2.25 (m, 8H), 2.26-2.4 (m, 4H), 3.6 (s, 3H), 3.80-3.98 (m, 1H), 4.0-4.3 (m, 4H), 6.54 (s, 1H), 7.24 (s, 1H), 7.3-7.45 (m, 3H), 7.45-7.52 (m, 2H), 7.65-7.75 (m, 2H); FABMS: 567 (M+H) Example 4 To a solution of (2S)-N- (4-hydroxy)-3-methoxy-2-nitrobenzoyl) pyrrolidine-2-carboxaldehyde diethylthioacetal of formula 2 (400 mg, 1 mmol) in dry acetone (20 ml) was added anhydrous potassium carbonate (690 mg, 5 mmol) and {2-(6-bromohexyl) oxy]-3,5-dichloro phenyl} (phenyl) methanone 1 (430 mg, 1 mmol). The reaction mixture was refluxed in an oil bath for 48 h and the reaction was monitored by TLC using ethyl acetate-hexane (4:6) as a solvent system. The potassium carbonate was then removed by suction filtration and the solvent was evaporated under vacuum to afford the crude product. This was further purified by column chromatography using ethyl acetate: hexane (3:7) as a solvent system to obtain the pure product 3 (614 mg, 82% yield). 1H NMR (CDCI3) 51.2-1.38 (m, 8H), 1.6-2.20 (m, 10H), 2.3-2.42 (m, 3H), 2.53-2.90 (m, 4H), 3.2-3.29 (m, 2H), 3.94 (s, 3H), 4.10 (t, 2H), 4.52-4.54 (m, 1H), 6.43 (s, 1H), 7.15-7.25 (s, 1H), 7.4-7.65 (m, 6H), 7.72-7.85 (d, 1H); FABMS: 749 (M+H) (2S)-N-{3-[2-benzoyl(4,6-dichlorophenyloxy)hexyloxy]}-5-methoxy-2-nitrobenzoyl} pyrrolidine-2-carboxaldehyde diethylthioacetal of formula 3 (500 mg, 0.66 mmol) was dissolved in methanol (10 ml), SnCI2.2H2O (754 mg, 3.33 mmol) was added and refluxed until the TLC indicated the completion of the reaction. The methanol was then evaporated in vacuum and the aqueous layer was then adjusted to pH 8 with 10% NaHCO3 solution and extracted with ethyl acetate (60 ml_). The combined organic phase was dried over Na2SO4 and evaporated under vacuum to afford the crude amino diethyl thioacetal 4 (460 mg, 96% yield), which was directly used in the next step. A solution of (2S)-N-{3-[2-benzoyl(4,6-dichlorophenyloxy)hexyloxy]}-5-methoxy-2-aminobenzoyl} pyrrolidine-2-carboxaldehyde diethylthioacetal 4 (400 mg, 0.55 mmol), HgCI2 (377 mg, 1.32 mmol) and CaCO3 (140 mg, 1.39 mmol) in acetonitrile-water (4:1) was stirred slowly at room temperature for overnight until complete consumption of starting material as indicated by the TLC. The clear organic supernatant liquid was extracted with ethyl acetate and washed with saturated 5% NaHCO3 (20 mL), brine (20 mL) and the combined organic phase was dried over Na2SO4. The organic layer was evaporated in vacuum and to afford a white solid, which was first eluted on a column chromatography with ethyl acetate to remove mercuric salts, and then with ethyl acetate to obtain the pure product 5c (185 mg, 56% yield). 1H NMR (CDCI3) 61.60-2.19 (m, 8H), 2.15-2.25 (m, 8H), 3.55-3.90 (m, 4H), 3.95 (s, 1H), 4.15-4.35 (m, 4H), 6.74 (s, 1H), 7.24 (s, 1H), 7.3-7.45 (m, 3H), 7.45-7.52 (m, 2H), 7.65-7.75 (m, 2H); FABMS: 595 (M+H) Example 5 To a solution of (2S)-N-(4-hydroxy)-3-methoxy-2-nitrobenzoyl)pyrrolidine-2-carboxaldehyde diethylthioacetal 2 (400 mg, 1 mmol) in dry acetone (20 ml) was added anhydrous potassium carbonate (690 mg, 5 mmol) and N1-[4-chloro-2-(2-chlorobenzoyl)phenyl]-2-chloro acetamide 1 (342 mg, 1 mmol). The reaction mixture was refluxed in an oil bath for 48 h and the reaction was monitored by TLC using ethyl acetate-hexane (4:6) as a solvent system. The potassium carbonate was then removed by suction filtration and the solvent was evaporated under vacuum to afford the crude product. This was further purified by column chromatography using ethyl acetate: hexane (3:7) as a solvent system to obtain the pure product 3 (578 mg, 82% yield). 1H NMR (CDCI3) 51.20-1.34.(m, 6H), 1.70-2.26 (m, 4H), 2.60-2.80 (m, 4H), 3.14-3.26 (m, 2H), 3.98 (s, 3H), 4.60-4.68 (t, 2H), 4.78 (m, 1H), 4.83(s, 2H), 6.82 (s, 1H), 7.25-765 (m, 5H), 7.80 (s, 1H), 8.80 (d, J = 4.76 1H), 12.20 (s, 1H); FABMS: 706 (M+H). 2S-N-{N1-[4-Chloro-2-(2-chlorobenoyl) phenyl]2-oxy acetamido]-5-methoxy-2-nitrobenzoyl} pyrrolidine-2-carboxaldehyde diethylthioacetal of formula 3 (500 mg, 0.7 mmol) was dissolved in methanol (10 ml_), SnCI2.2H2O (800 mg, 3.54 mmol) was added and refluxed until the TLC indicated the completion of the reaction. The methanol was then evaporated in vacuum and the aqueous layer was then adjusted to pH 8 with 10% NaHCO3 solution and extracted with ethyl acetate (60 ml_). The combined organic phase was dried over Na2SO4 and evaporated under vacuum to afford the crude amino diethyl thioacetal 4 (460 mg, 96% yield), which was directly used in the next step. A solution of 2S-N-{N1- [4-chloro-2-(2-chlorobenoyl) phenyl]2-oxy acetamido] -5-methoxy-2-aminobenzoyl}pyrrolidine-2-carboxaldehyde diethylthioacetal 4 (400 mg, 0.7 mmol), HgCI2 (384 mg, 1.42 mmol) and CaCO3 (149 mg, 1.47 mmol) in acetonitrile-water (4:1) was stirred slowly at room temperature for overnight until complete loss of starting material as indicated by the TLC. The clear organic supernatant liquid was extracted with ethyl acetate and washed with saturated 5% NaHCO3 (20 ml), brine (20 ml) and the combined organic phase was dried over Na2SO4. The organic layer was evaporated in vacuum and to afford a white solid, which was first eluted on a column chromatography with ethyl acetate to remove mercuric salts, and then with ethyl acetate to obtain the pure product 5c (179 mg, 55% yield). 1H NMR (CDCI3) 8 1.20-1.35 (m, 6H), 1.7-2.25 (m, 4H), 3.14-3.26 (m, 2H), 3.98 (s, 3 H), 4.60-4.68 (m, 1H), 4.78 (s, 1H), 4.83 (s, 2H), 6.82 (s, 1H), 7.25-7.35 (m, 1H), 7.80 (m, 1H), 8.80 (d, 1 H, J = 4.33 Hz), 12.2 (s, 1H); FABMS: 552 (M+H). Biological Activity: some of in vitro biological activity studies were carried out at the National Cancer Institute, Marryland, USA. Cytotoxicity: The compounds 5a) 7-methoxy-8-{3-[2-benzoyl-(4-chlorophenyloxy)propoxy]}-(11aS)-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepine-5-one,5b)7-metho-xy-8-{4-[2-benzoyl-(4-chlorophenyloxy)butoxy]}-(11 aS)-1,2,3,11 a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepine-5-one.5c)7-methoxy-8-{4-[2-benzoyl-(4,6-dichloro phenyl-oxy)butoxy]}-(11aS)-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4] benzo diazepine-5-one,5d)7-methoxy-8-{6-[2-benzoyl-(4,6-dichloro phenyloxy)hexyloxy]} -(11 aS)-1,2,3,11 a-tetra-hydro-5H-pyrrolo[2,1-c][1,4]benzodiazepine-5-one,5e)7-methoxy-8-{N1-[4-chloro-2-(2-chlorobenzoyl)phenyl]2-oxyacetamido}-(11aS)-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c] [1,4]benzodiazepine-5-one, were evaluated for in vitro anticancer activity against nine human tumour cells derived from nine cancer types (colon, prostate, oral, lung, cervix and breast cancer) as shown in (Table 1,2 and 3) 5e was evaluated for in vitro anticancer activity against sixty human tumour cells derived from nine cancer types (leukemia, non-small-cell lung, colon, CNS, melanoma, ovarian, prostate, and breast cancer) as shown in (Table 1 and 2). For the compound, dose response curves for each cell line were measured at a minimum of five concentrations at 10 fold dilutions. A protocol of 48 h continuous drug exposure was used and a sulforhodamine B (SRB) protein assay was used to estimate cell viability or growth. The concentration causing 50% cell growth inhibition (GI50), total cell growth inhibition (TGI 0% growth) and 50% cell death (LC50, -50% growth) compared with the control was calculated. The mean graph midpoint values of logio TGI and log 10 LC50 as well as log10 GI50 for 5e is listed in Table 1 and 2). As demonstrated by mean graph pattern, compound 5e exhibited an interesting profile of activity and selectivity for various cell lines. The mean graph mid point of logio TGI and logio LC50 showed similar pattern to the logio GI50 mean graph mid points. Table 1. Log10GI50 logioTGI and log10LC50 mean graphs midpoints (MG_MID) of in vitro cytotoxicity data for the representative compounds against human tumour cell lines (Table 1 Removed) Table 2. Log10LC50 concentration in mol/L causing 50% lethality) values for the representative compound 5e (Table 2 Removed) Each cancer type represents the average of six to nine different cancer cell lines. In vitro evaluation of cytotoxic activity. The compound 5a, 5b, 5g 5i and 5j were evaluated for in vitro anticancer activity against nine human tumour cells derived from six cancer types (colon, prostate, oral, lung, cervix and breast cancer) as shown in Table 3. Compound 5a, 5b and 5g shows promising cytotoxicity against some cancer cell lines (Table 3). Compounds 5a, 5b, 5g 5i and 5j have been evaluated for their in vitro cytotoxicity in selected human cancer cell lines of colon (Colo205), lung (Hop-62), cervix (SiHa), prostate (DU145, PC3), oral (DWD, HT1080), and breast (MCF7, Zr-75-1) origin. A protocol of 48 h continuous drug exposure has been used and an Adriamycin (ADR) protein assay has been used to estimate cell viability or growth. The results are expressed as percent of cell growth determined relative to that of untreated control cells Compounds 5b, 5g, 5i and 5j exhibited less than 20% cell growth at pg/mL concentration in some cancer cell lines. Compound 5a, Colo205 cell growth by 83%, DU145 cell growth by 84%, DWD cell growth by 70%, Hop62 cell growth by 87 %, HT1080 cell growth by 80%, MCF7 cell growth by 73%, PC3 cell growth by 91%, SiHa cell growth by 67%,Zr-75-1 cell growth by 76% .Compound 5b, Colo205 cell growth by 20%, DU145 cell growth by 64%, DWD cell growth by 70%, Hop62 cell growth by 78 %, HT1080 cell growth by 72%, MCF7 cell growth by 69%, PCS cell growth by 72%, SiHa cell growth by 20%,Zr-75-1 cell growth by 20%. Compound 5g, Colo205 cell growth by 50%, DU145 cell growth by 20%, DWD cell growth by 94%, Hop62 cell growth by 84 %, HT1080 cell growth by 94%, MCF7 cell growth by 71%, PC3 cell growth by 86%, SiHa cell growth by 74%,Zr-75-1 cell growth by 72%. Compound 5i, DU145 cell growth by 42%, Hop62 cell growth by 60 %, HT1080 cell growth by 70%, Compound 5j, Colo205 cell growth by 44%, DU145 cell growth by 57%, DWD cell growth by 65%, Hop62 cell growth by 53 %, HT1080 cell growth by 71%, PC3 cell growth by 81%, SiHa cell growth by 77%. Table 3. The percentage cell growth data for representative benzophenone-PBD hybrids ADR = Adiramycin is the control drug (Table 3 Removed) Thermal denaturation studies Compounds were subjected to thermal denaturation studies with duplex-form calf thymus DNA (CT-DNA) using an adaptation of a reported procedure. Working solutions in aqueous buffer (10 mM NaH2PO4/Na2HPO4, 1 mM Na2EDTA, pH 7.00+0.01) containing CT-DNA (100 μrn in phosphate) and the PBD (20 μm) were prepared by addition of concentrated PBD solutions in DMSO to obtain a fixed [PBD]/[DNA] molar ratio of 1:5. The DNA-PBD solutions were incubated at 37 °C for 0, 18, and 36 h prior to analysis. Samples were monitored at 260 nm using a Beckman DU-7400 spectrophotometer fitted with high performance temperature controller, and heating was applied at 1 °C min-1 in the 40-90 °C range. DNA helix coil transition temperatures (Tm) were obtained from the maxima in the (dA260)/dT derivative plots. Results are given as the mean ± standard deviation from three determinations and are corrected for the effects of DMSO co-solvent using a linear correction term. Drug-induced alterations in DMA melting behaviour are given by: ATm=Tm(DNA+PBD)-Tm (DNA alone), where the Tm value for the PBD-free CT-DNA is 69.0 ± 0.01. The fixed [PBD]/[DNA] ratio used did not result in binding saturation of the host DNA duplex for any compound examined.Compound 5a, 5b, 5g 5i and 5j at 0 hr,18 hr and 36 hr gradually increased at 37°C. Table 4. Thermal denaturation data of C8-linked benzophenone hybrids of pyrrolo[2,1-c]-[1,4]benzodiazepine with calf thymus (CT) DNA (Table 4 Removed) aFor CT-DNA alone at pH 7.00 ± 0.01, Tm = 69.6 °C ± 0.01 (mean value from 10 separate determinations), all A7m values are ± 0.1 - 0.2 °C. b For a 1:5 molar ratio of [ligand]/[DNA], where CT-DNA concentration = 100 μM and ligand concentration = 20 μM in aqueous sodium phosphate buffer [10 mM sodium phosphate + 1 mM EDTA, pH 7.00 ±0.01]. ADVANTAGES OF THE INVENTION 1. The present invention provides a new pyrrolo[2,1-c] [1,4]benzodiazepine hybrids useful as antitumour agents. 2. It also provides a process for the preparation of novel pyrrolo[2,1-c] [1,4] benzodiazepine hybrids We claim 1. Novel benzophenone linked pyrrolo[2,1-c][1,4] benzodiazepine hybrid of general formula 5 (Formula Removed) wherein, n = 1-5. 2. Novel benzophenone linked pyrrolo [2,1-c][1,4] benzodiazepine hybrid as claimed in claim 1 is represented by the group of the following compounds: 7-methoxy-8-{3-[2-benzoyl-(4-chlorophenyloxy)propoxy]}-(11 aS)-1,2,3,11 a-tetra hydro-5H-pyrrolo[2,1-c][1,4]benzodiazepine-5-one(5a);7-methoxy-8-{4-[2-benzoyl- (4-chlorophenyloxy)butoxy]}-(11aS)-1,2,3,11 a-tetrahydro-5H-pyrrolo[2,1- c][1,4]benzodiazepine-5-one(5b);7-methoxy-8-{5-[2-benzoyl-(4chlorophenyloxy) pentoxy]}-(11aS)-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepine-5- one(5c);7-methoxy-8-{3-[2-benzoyl-(4-chloro-6-methylphenyloxy)propoxyl}-(11aS) -1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepine-5-one(5d);7-methoxy- 8-{3-[2-benzoyl-(4-chloro-6-methylphenyloxy)butoxy]}-(11aS)-,2,3,11 a-tetra hydro- 5H-pyrrolo[2,1-c][1,4] benzodiazepine-5-one (5e); 7-methoxy-8-{3-[2-benzoyl-(4- chloro-6-methylphenyloxy)pentoxy]}-(11aS)-1,2,3,11 a-tetra hydro-5H-pyrrolo[2,1- c][1,4]benzodiazepine-5-one(5f);7-methoxy-8-{3-[2-benzoyl-(4,6- dichlorophenyloxy)propoxyl}-(11aS)-1,2,3,11a-tetra hydro-5H-pyrrolo[2,1-c][1,4] benzodiazepine-5-one (5g); 7-methoxy-8-{3-[2-benzoyl-(4,6-dichlorophenyloxy) butoxy]}-(11 aS)-1,2,3,11 a-tetrahydro-5H-pyrrolo[2,1 -c][1,4] benzodiazepine-5-one (5h);7-methoxy-8-{3-[2-benzoyl-(4,6-di-chlorophenyloxy)hexyloxy]}-(11aS)- 1,2,3,11 a-tetra hydro-5H-pyrrolo[2,1-c][1,4] benzodiazepine-5-one (5i 7-Methoxy- 8-{N1-[4-chloro-2-(2-chlorobenzoyl)phenyl]2-oxyacetamido}-(11aS)-1,2,3,11a- tetrahydro-5H -pyrrolo[2,1-c][1,4]benzodiazepine-5-one (5j); 7-Methoxy-8-{N1-[4- chloro-2-(2-chlorobenzoyl)phenyl]2-oxypropinamido}-(11aS)-1,2,3,11a-tetrahydro-5H -pyrrolo[2,1-c][1,4]benzodiazepine-5-one 5k); 7-Methoxy-8-{N1-[4-chloro-2-(2-chlorobenzoyl)phenyl]2-oxybutanamido}-(11aS)-1,2,3,11a-tetrahydro-5H-pyrrolo [2,1-c][1,4]benzodiazepine-5-one 51). Novel benzophenone linked pyrrolo [2,1-c][1,4] benzodiazepine hybrid as claimed in claim 1, wherein the structural formula of the representative compound are: (Formula Removed) 3. A process for the preparation of benzophenone linked pyrrolo[2,1-c][1,4]benzodiazepine hybrid of formula 5, (Formula Removed) wherein n = 1, 2, 3 or 4, the said process comprising the steps of: a) reacting(2S)-N-(4-hydroxy-3-methoxy-2-nitrobenzoyl)pyrrolidine-2-carbox aldehyde diethylthioacetal of formula 2 (Formula Removed) [2-(n-bromoalkyl)-n-chlorophenyl)(phenyl) methanone or acetamide of formula 1, (Formula Removed) where in X1, X2 and X3 is selected from the group consisting of H, CI and CH3; Y is selected from O and NH; Z is selected from C=O and CH2; n = 1to 4, in an aprotic water miscible organic solvent, in the presence of anhydrous mild inorganic base, under refluxing temperature in an oil bath, for a period of about 48 hrs, followed by the removal of inorganic base by filtration and evaporating the organic solvent to obtain the resultant crude product and purifying it by known method to obtain the desired product of (2S)-N-{n benzoyl (phenyloxy) alkoxy / [(n benzoyl) phenyl] 2-oxyacetamido]5-methoxy-2-nitrobenzoyl}-pyrrolidine-2-carboxaldehyde diethylthioacetal of formula 3, (Formula Removed) wherein X1, X2 and X3 is selected from the group consisting of H, CI and CH3; Y is selected from O and NH; Z is selected from C=O and CH2; n = 1 to 4, b) reducing (2S)-N-{n benzoyl (phenyloxy)alkoxy / (n benzoyl) phenyl] 2-oxy acetamido]-5-methoxy-2-nitrobenzoyl}-pyrrolidine-2-carboxaldehyde diethylthioacetal of formula 3 with anhydrous tin chloride, in an alcohol, under reflux, followed by the evaporation of alcohol and adjusting the pH of the resultant product layer to about 8 by using a base, followed by extraction with ethyl acetate and washing the combined organic phase with brine solution and evaporating the solvent to obtain the desired (2S)-N-{n benzoyl (phenyloxy)alkoxy/[(n-benzoyl)phenyl]2-oxyacetamido]-5-methoxy- 2-amino benzoyl}-pyrrolidine-2-carboxaldehyde diethylthioacetal of formula 4, (Formula Removed) where in X1, X2 and X3 is selected from the group consisting of H, CI and CH3; Y is selected from 0 and NH; Z is selected from C=O and CH2; n = 1 to 4, c) reacting(2S)-N-{n-benzoyl(phenyloxy)alkoxy/[(n-benzoyl)phenyl]-2-oxy acetamido]-5-methoxy-2-aminobenzoyl}-pyrrolidine-2-carboxaldehyde diethylthioacetal of formula 4 with mercurous chloride, in a mixture of water and organic solvent, in the presence of mild inorganic, under stirring, at a temperature of about 20-30°C, for a period of 8-12 hrs, followed by the extraction of yellow organic supernatant and washing with sodium bi carbonate and brine, respectively, and evaporating the organic layer, under reduced pressure to obtain the desired product of benzophenone linked pyrrolo[2,1-c][1,4] benzodiazepine hybrid of formula 5. 4. A process as claimed in claim 3, wherein the mild inorganic base used in steps (a) & (b) is calcium carbonate. 5. A process as claimed in claim 3, wherein the aprotic organic solvent used in step (a) is acetone and acetonitrile. 6. A process as claimed in claim 3, wherein the organic solvent used in step (c) is acetonitrile and acetone. 7. A process as claimed in claim 3, wherein the alcohol used in step (b) is selected from methanol and ethanol. 8. A process as claimed in claim 3, wherein the compounds of formula 5 obtained are represented by a group of the following compounds: 7-methoxy-8-{3-[2-benzoyl-(4-chlorophenyloxy)propoxyl}-(11 aS)-1,2,3,11 a-tetra hydro-5H-pyrrolo[2,1-c][1,4]benzodiazepine-5-one(5a);7-methoxy-8-{4-[2-benzoyl-(4-chlorophenyloxy)butoxy]}-(11aS)-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4] benzodiazepine-5-one(5b);7-methoxy-8-{5-[2-benzoyl-(4chlorophenyloxy) pentoxyl}-(11aS)-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepine-5-one(5c);7-methoxy-8-{3-[2-benzoyl-(4-chloro-6-methylphenyloxy)propoxy]}-(11aS) -1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepine-5-one(5d);7-methoxy-8-{3-[2-benzoyl-(4-chloro-6-methylphenyloxy)butoxy]}-(11aS)-1,2,3,11 a-tetra hydro-5H-pyrrolo[2,1-c][1,4] benzodiazepine-5-one (5e); 7-methoxy-8-{3-[2-benzoyl-(4-chloro-6-methylphenyloxy) pentoxy]}-(11 aS)-1,2,3,11 a-tetra hydro-5H-pyrrolo[2,1-c][1,4] benzodiazepine-5-one (5f); 7-methoxy-8-{3-[2-benzoyl-(4,6-dichlorophenyloxy)propoxy]}-(11aS)-1,2,3,11 a-tetra hydro-5H-pyrrolo[2,1-c][1,4] benzodiazepine-5-one (5g); 7-methoxy-8-{3-[2-benzoyl-(4,6-dichlorophenyloxy) butoxy]}-(11aS)-1,2,3,11 a-tetra hydro-5H-pyrrolo[2,1-c][1,4] benzodiazepine-5-one (5h); 7-methoxy-8-{3-[2-benzoyl-(4,6-di-chlorophenyloxy)hexyloxyl}-(11aS)-1,2,3,11 a-tetra hydro-5H-pyrrolo[2,1-c][1,4] benzodiazepine-5-one (5i);7-Methoxy-8-{N1-[4-chloro-2-(2-chlorobenzoyl)phenyl]2-oxyacetamido}-(11aS)-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepine-5-one(5j);7-Methoxy-8-{N1-[4- chloro-2-(2-chlorobenzoyl)phenyl]2-oxypropinamido}-(11aS)-1,2,3,11a-tetrahyclro-5H-pyrrolo[2,1-c][1,4]benzodiazepine-5-one 5k);7-Methoxy-8-{N1-[4-chloro-2-(2-chlorobenzoyl)phenyl]2-oxybutanamido}-(11 aS)-1,2,3,11 a-tetrahydro-5H-pyrrolo [2,1-c][1,4] Benzodiazepine -5-one (51).

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1823-del-2006-Correspondence Others-(30-11-2012).pdf

1823-del-2006-correspondence-others.pdf

1823-del-2006-description (complete).pdf

1823-del-2006-drawings.pdf

1823-del-2006-form-1.pdf

1823-del-2006-form-2.pdf

1823-del-2006-Form-3-(01-06-2012).pdf

1823-DEL-2006-Form-3-(28-06-2012).pdf

1823-del-2006-form-3.pdf

1823-del-2006-form-5.pdf

1823-DEL-2006-Petition-137-(28-06-2012).pdf