Title of Invention

"A PROCESS FOR PREPARING PURIFIED 4-(2-METHYL-1-IMIDAZOLYL)-2,2-DIPHENYLBUTANAMIDE

Abstract

The industrial production of 4-(2-methyl-l-imidazolyl)-2,2-diphenylbutanamide, a urinary incontinence remedy, necessitates elimination of problems concerning the use of a synthetic adsorbent, e.g., HP-20, the efficiency of operation with the same, purification efficiency, etc. An acid salt, e.g., hydrochloride or phosphate, of 4-(2-methyl-l-imidazolyl)-2,2- diphenylbutanamide or a hydrate of any of these salts is used as an intermediate. This intermediate is neutralized and then purified. Thus, high-purity 4-(2-methyl -l-imidazolyl)-2,2-diphenylbutanamide is easily obtained in satisfactory yield. The industrial-scale production process has been thus established.

Full Text

SPECIFICATION Process for preparing antagonist against muscarine receptor and its intermediate Technical field The present invention relates to a novel preparative intermediate of 4-(2-methyl-l-imidazolyl)-2, 2-diphenylbutanamide (hereinafter abbreviated as "Compound (1)"), being a selective muscarinic receptor antagonist , and a preparative process of Compound (1) using that intermediate. Background technology Compound (Formula Removed) is an imidazole derivative having anticholinergic effect, above all, selective and potent antagonistic function against muscarine receptor, and it is known that it is useful for medicinal uses for the therapies of motility disorders in digestive tract such as irritative colon syndrome, diverticulosis, functional diarrhea, esophageal achalasia and cardiospasm, • therapies of convulsion of biliary tract or urinary tract and incontinence of urine, etc., and therapy of chronic respiratory tract-obstructive disease, and the like (compounds of Patent document 1 and Example 11 of Patent document 2). Also, the preparative processes of Compound (1) are disclosed concretely (Patent documents 2 and 3, Nonpatent document 1). However, in the processes disclosed therein, synthetic adsorbent such as HP-20 must be used on purification, which has problems on the operativities, purification efficiencies, etc. from the viewpoint of intending industrial scale-up. Hence, there has been a necessity for further improvement and contrivance to find out a preparative process that adapts to the practical production. [Patent document 1] W09515951 pamphlet [Patent document 2] Jpn. Kokai Tokkyo Koho JP 007,215,943 [Patent document 3] Jpn. Kokai Tokkyo Koho JP 2003-201281 {Nonpatent document 1] Bioorg. Med. Chem., 7(6), 1151-1161 (1999). -. j. Disclosure of the invention For keeping the industrial production of high-quality Compound (1) as a medicinal drug, it is necessary to solve the problems of improvements in the operability and purification efficiency in the preparative process that adapt to practical production level, or the problems of possibility of the synthetic adsorbent being mixed into the original drug. As a result of diligent studies for solving the problems aforementioned, the inventors have found that, by separating and purifying salt of hydrochloride, phosphate or the like of 4-(2-methyl-l-imidazolyl) -2,2-diphenylbutanamide, or hydrate of that salt, as a preparative intermediate, and then, by neutralizing, Compound (1) can be prepared by simple operation and with good purification efficiency, leading to the completion of the invention. Namely, the invention relates to 1) salt of 4-(2-methyl-l-imidazolyl)-2,2-diphenylbutanamide, or hydrate of that salt, 2) hydrochloride or phosphate of 4-(2-methyl-l-imidazolyl)-2,2- diphenylbutanamide , or hydrate of that acid salt, 3) acid salt or hydrate of that acid salt of said 2), characterized in that it is a preparative intermediate of 4- (2-methyl-l- imidazolyl) -2,2-diphenylbutanamide, 4)/acid salt or hydrate of that acid salt of said 2), characterized in that it is a preparative intermediate for preparing purified product of 4-(2-methyl-l-imidazolyl)-2,2-Diphenylbutanamide, which is easy for isolation and purification, 5) a process for preparing isolated and purified product of acid salt or hydrate of that acid salt of said 2), wherein inorganic acid or organic acid is reacted with crude crystals of 4-(2-methyl -1-imidazolyl)-2,2-diphenylbutanamide to isolate and purify the acid salt or hydrate of that acid salt, 6) a process for preparing purified 4-(2-methyl-l-imidazolyl)- 2,2-diphenylbutanamide by neutralizing the isolated and purified product of acid salt or hydrate of that acid salt of said 5) with alkali, 7) a process for preparing purified product of 4-(2-methyl-l -imidazolyl)-2,2-diphenylbutanamide, wherein the acid salt of 4-(2-methyl-l-imidazolyl)-2,2-diphenylbutanamide , or hydrate of that acid salt isolated in said 5) is recrystallized from alcohol and ethyl acetate, followed by neutralization, by using hydroxide of alkali metal, and the crystals obtained are recrystallized from alcohol or water-containing alcohol, and 8) the acid salt or hydrate of that acid salt of said 2) or 3), that is possible to provide 4-(2-methyl-l-imidazolyl)-2,2- diphenylbutanamide with high quality in good yield only by simple recrystallization. According to the invention, a preparative process for 4-(2-methyl-l-imidazolyl)-2,2-diphenylbutanamide that is advantageous industrially is established and it is possible to provide it as a high-purity and high-quality medicinal drug. Best mode for carrying out the invention The salt of 4-(2-methyl-l-imidazolyl)-2,2-diphenylbutanamide means addition salt with inorganic acid such as hydrochloric acid, sulfuric acid, hydrobromic acid or phosphoric acid, or addition salt with organic acid such as maleic acid, fumaric acid, acetic acid, oxalic acid, tartaric acid or benzenesulfonic acid. Thereamong, hydrochloride or phosphate is preferable. In the invention, crude crystals 4-(2-methyl-l-imidazolyl) -2,2-diphenylbutanamide are obtained according to Patent document 3, these are dissolved into alcohol such as methanol, ethanol, propanol or 2-propanol, equimolar acid such as hydrochloric acid or phosphoric acid is added, then, organic solvent such as ethyl acetate is added, or crystallization is made by using an equimolar mixed solution of acid such as hydrochloric acid or phosphoric acid, alcohol and ethyl acetate, thereby isolating 4-(2-methyl-l-imidazolyl)-2,2-diphenylbutanamide as an acid salt of hydrochloride, phosphate or the like, or hydrate thereof, and then, after neutralization by us ing alkali metal hydroxide, it is recrystallized from alcohol such as methanol, ethanol, propanol or 2-propanol or water-containing alcohol thereof. Through these steps, it has been found that Compound (1) with high-purity can be obtained by simple operation and in good purification efficiency, leading to the completion of industrial scale preparative process (scheme). (Formula Removed) The inventive acid salt of hydrochloride, phosphate or the like of 4-(2-methyl-l-imidazolyl)-2,2-diphenylbutanamide, or hydrate of that salt is a novel compound with no concrete disclosure, and its usefulness has also been unknown. By using this novel salt or hydrate of'that salt, it has been found that the purification efficiency improves and the purifying operation also becomes simple, thereby completing the invention as an industrial process. If using the inventive acid salt of hydrochloride, phosphate or the like of 4-(2-methyl-l-imidazolyl)-2,2-diphenylbutanamide, or hydrate of that salt, then original drug of high-quality Compound (1) can be obtained in good yield, only by simple recrystallization of crude product without using synthetic adsorbent. According to the invention, excellent industrial production process of Compound (1) has been provided. In following, the invention will be illustrated based on concrete examples, but the invention is not confined to these examples. Referential example 1 Crude crystals of 4-(2-methylimidazole-l-yl)-2,2-diphenylbutanamide Amixture of 500g (1.67mol) of 4-bromo-2,2-diphenylbutyronitrile , 685g (8.34mol) of 2-methylimidazole and 250mL of dimethylsulfoxide (DMSO) was stirred for 5 hours at 95 to 105°C, and then cooled with ice water. After 2L of ethyl acetate and 2L of water were added therein at an inner temperature of 39°C, and the mixture was stirred for 5 minutes, organic layer was separated. After washing the organic layer with 2L of water and then 2L of 2.5% acetic acid, the organic layer, was concentrated under reduced pressure. The residual oil was dissolved in 2L of ethanol and to this solution a solution of 192g (1.67mol) of 85% phosphoric acid and 1L of ethanol was added dropwise at an inner temperature of 31°C under stirring, and this dropwise addition was interrupted when the solution became milky white (about 500mL were added dropwise). After confirming the precipitation of crystals by stirring for 30 minutes, remaining phosphoric acid solution was added dropwise, and successively the solution was stirred for 16 hours at an inner temperature of 30°C. The precipitated crystals were collectedby filtration and, after washing them with 1L of ethanol, the crystals were dried for 5 hours at 60°C under reduced pressure (vacuum pump) to obtain 496g (74.5%) of 4-(2-methylimidazole -1-yl)-2,2-diphenylbutyronitrile phosphate. Out of these crystals, lOOg were completely dissolved with stirring into a mixture of l00mL of purified water and 400mL of 2-propanol and to this solution 500mL of 2-propanol were added under stirring, followed by cooling with ice water. After stirring this solution for 1 hour at an inner temperature of below 15°C, the precipitated crystals were collected by filtration which were then washed with l00mL of 2-propanol, then with lOOmL of ethyl acetate. The crystals were dried for 17 hours at 60°C under reduced pressure (vacuum pump) to obtain 91.3g (Total yield: 68%) of purified product of 4-(2-methylimidazole-l-yl) -2,2-diphenylbutyronitrile phosphate as white crystalline powder with hygroscopicity. After a mixture of 80. Og (200mmol) of the purified product thus obtained, 132g(2.02mol) of 86% potassium hydroxide and 400mL of 2-propanol was refluxed for 5 hours under an atmosphere of argon, the mixture was cooled with ice water. Under stirring, 800mL of 2mol/L hydrochloric acid were added into the mixture at an inner temperature of 30°C (temperature was raised to 50°C) to crystallize out of the mixture, and, after stirring for 1 hour at an inner temperature of below 15°C, the crystals were collected from the solution by filtration. These crystals were washed with a mixture of 30mL of 2-propanol and 60mL of purified water, then with each 250mL of purified water five times (fifth washed solution: pH 8.80). The crystals were dried for 16 hours at 40°C in blower to obtain 55. 2g (86.4%) of crude crystals. Into a mixed solution of 200mL of 95% 2-propanol, 27. 6g out of these were dissolved completely by heating, which was then stirred for 1 hour at room temperature to crystallize out of the solution, followed by cooling with ice water. After stirring the solution for 1 hour at an inner temperature of below 15°C, the precipitated crystals were collected by filtration and washed with lOmL of 2-propanol. The crystals were dried for Shours at 60°C under reduced pressure (vacuum pump) to obtain 25.7g(Total yield: 80%) of crude crystals of 4-(2-methylimidazole-l-yl)-2,2-diphenylbutanamide as white crystalline powder. Example 1 4- (2-Methyl-l-imidazolyl) -2,2-diphenylbutanamide hydrochloride Into a mixed solution of 5mL of concentrated hydrochloric acid/95mL of 2-propanol, 19.2g (60.0mmol) of crude crystals of 4-(2-methyl-1-imidazolyl)-2,2-diphenylbutanamide obtained in Referential example 1 were dissolved completely by heating, then lOOmL of ethyl acetate were added into the solution, and it was stirred for 1 hour at room temperature to crystallize out of the solution, followed by cooling with ice water. After stirring it for 1 hour at an inner temperature of below 15°C, the crystals were collected by filtration and washed with lOmL of ethyl acetate. After completely dissolving the wet crystals into lOOmL of 95% 2-propanol by heating, lOOmL of ethyl acetate were added into the solution, and it was stirred for 1 hour at room temperature to crystallize out of the solution, followed by cooling with ice water. After stirring it for 1 hour at an inner temperature of below 15°C, the crystals were collected by filtration and washed with lOmL of ethyl acetate. The crystals were dried for 3 hours at 60°C under reduced pressure (vacuum pump) to obtain 16.9g(79.3%) of 4-(2-methyl-l- imidazolyl)-2,2-diphenylbutanamide hydrochloride. After completely dissolving this hydrochloride into SOrnL of 90% 2-propanol by heating, 160mL of ethyl acetate were added into the solution, and it was stirred for 1 hour at room temperature to crystallize out of the solution, followed by cooling with ice water. After stirring for 1 hour at an inner temperature of below 15°C, the crystals were collected by filtration and washed with lOmL of ethyl acetate. The crystals were dried for 3 hours at 60°C under reduced pressure (vacuum pump) to obtain 14.5g (Total yield: 68%) of 4-(2-methyl-l-imidazolyl)-2,2-diphenylbutanamide hydrochloride as white crystalline powder. mp 205-208CC (Hot plate method) EI-MS m/z: 319(M+) Anal-. Calcd. C20H2lN3O.HCl: C, 67.50; H, 6.23; N, 11.81. Found: C, 67.25; H, 6.26; N, 11.83. 1H-NMR(DMSO-d6,400MHz)6:2.38(3H,s),2.75-2.79(2H,m),3.73-3.77 (2H,m),6.81(lH,s),7.28-7.39(10H,m),7.44(lH,s),7.50(lH,d, J=2.4Hz), 7.55(lH,d,J=2.0Hz), 14.41(lH,br s). Example 2 4- (2-Methyl-l-imidazolyl) -2,2-diphenylbutanamide In 71mL of purified water, 7.12g (20.0mmol) of 4-(2-methyl-1-imidazolyl) -2,2-diphenylbutaneamide hydrochloride obtained in Example 1 were dissolved, and 0.71g of activated charcoal were added into the solution. After stirring it for 1 hour, the mixture was filtered through 2. 1cm-size Kiriyama funnel laid with 1. 00g of cellulose powder and washed with 3mL of purified water. To the filtrate and washed solution, 56mL of ethanol were added, and, under stirring, lOmL of 2mol/L sodium hydroxide solution were added into the solution to neutralize and crystallize out of the solution. The suspension was stirred for 1 hour at room temperature and then cooled with ice water. After stirring it for 1 hour at an inner temperature of below 15°C, the crystals were collected by filtration and washed with lOmL of 40% ethanol, then with each lOOmL of purified water five times (fifth washed solution: pH 8.57). The crystals were dried for 5 hours at 60°C under reduced pressure (vacuum pump) to obtain 5.88g (92.0%) of crystals. Into 3 OmL of 90% ethanol, 5.78g out of these were dissolved completely by heating, which was stirred for 1 hour at room temperature to crystallize out of solution, followed by cooling it with ice water. After stirring it for 1 hour at an inner temperature of below 15°C, the crystals were collected by filtration and washed with 5mL of ethanol. The crystals were dried for 3 hours at 60°C under reduced pressure (vacuum pump) to obtain 5.22g(Total yield: 83%) of 4-(2-methyl- 1-imidazolyl) -2,2-diphenylbutanamide as white crystalline powder. mp 191-193°C (Hot plate method) EI-MS m/z: 319 (M+) IR(KBr) cm-1: 3333, 3047, 1673, 1499. Anal. Calcd. C2QH21N30: C, 75.21; H, 6.63; N, 13.16. Found: C, 75.34; H, 6.66; N, 13.37. 1H-NMR(DMSO-d6,400MHz)6:2.01(3H,s),2.60-2.64(2H,m),3.51-3.55(2H,m),6.67(lH,d,J=1.2Hz),6.90(lH,brs),6.92(lH,d,J=1.2Hz), 7.25-7.37(llH,m). [Conditions for measuring HPLC] Detector: An ultraviolet absorption photometer (wavelength: 227nm). Column: A stainless steel column of 4.6mm in inside diameter and 15cm in length, packed with octadecylsilanized silicagel for liquid chromatography, 5µm in particle diameter(Inertsil ODS-3V). Column temperature: A constant temperature of about 30°C. Mobile phase A : Dissolved 2.16g of sodium 1-octanesulfonate in diluted phosphoric acid (1 in 1000) to make lOOOmL. Mobile phase B : Acetonitrile. Mobile phase C : Methanol. During 40 minutes after injection of sample, feeding was controlled on linear concentration gradient from mixed solution of mobile phase A/mobile phase B/mobile phase C (12:5:3) to mixed solution of mobile phase B/mobile phase A/mobile phase C (12:5:3), and, during next 10 minutes, mixed solution of mobile phase B/mobile phase A/mobile phase C (12:5:3) was fed. Flow rate: This was adjusted so as the retention time of KRP-197 to become 9 to 10 minutes (ca. ImL/min). Time span of measurement : about 5 times as long as the retention time of KRP-197 (about 45 minutes). HPLC purity : 100%. Example 3 4- (2-Methyl-l-imidazolyl)-2,2-diphenylbutanamide phosphate Into 11 times volume of 90% 2-propanol, crude crystals of 4- (2-methyl-l-imidazolyl) -2,2-diphenylbutanamide obtained similarly to Referential example 1 were dissolved by heating. After equimolar 85% phosphoric acid was added, the precipitated insolubles were dissolved by heating, and the solution was stirred for 1 hour at room temperature, then for 1 hour under cooling with ice water to make phosphate crystals, followed by filtration. After drying, these were recrystallized from ten times volume 70% 2-propanol. The crystals were dried for 3 hours at 60°C under reduced pressure (vacuum pump) toobtain37.3g (sum-upyield: 89%) of 4-(2-methyl-l-imidazolyl) -2,2-diphenylbutanamide phosphate. mp 219-220°C (Hot plate method) EI-MS m/z: 319 (M+) Anal. Calcd. C20H21N30 . H3P04 • 0.8H20: C, 55.63; H, 5.98; N, 9.73. Found: C, 55.52; H, 5.82; N, 9.69. 1H-NMR(DMSO-d6,400MHz )6:2.13(3H,s),2.65-2.69(2H,m),3.58- 3.62 (2H,m),6.87(lH,brs),6.90(lH,d,J=1.5Hz),7.10(lH,d,J=1.5Hz), 7.26-7.43(llH,m), 8.41(2H,br). Example 4 4-(2-Methyl-l-imidazolyl)-2,2-diphenylbutanamide Into a mixture of 32mL of Imol/L hydrochloric acid and l0lmL of purified water, 13.3g (32.0mmol) of 4-(2-methyl-l- imidazolyl)-2,2-diphenylbutanamide phosphate obtained in Example 3 were dissolved, and 1.33g of activated charcoal were added. After stirring for 1 hour, the mixture was filtered through 4cm-size Kiriyama funnel, in which 2.66g of cellulose powder was laid and washed with 13mL of purified water. To the filtrate and washed solution, 130mL of ethanol were added, and, under stirring, 48mL of 2mol/L sodium hydroxide solution were added to neutralize the solution and crystallize out of the solution. After stirring for 1 hour at an inner temperature of below 15°C under cooling with ice water, the crystals were collected by filtration and washed with 50mL of 40% ethanol, then with each 200mL of purified water five times :(fifth washed solution: pH 8.80). Into 53mL of ethanol, 10. 6g of wet crystals were dissolved completely by heating, and the solution was stirred for 1 hour at room temperature to crystallize out of the solution, followed by cooling with ice water. After stirring for 1 hour at an inner temperature of below 15°C, the crystals were collected by filtration and washed with 5mL of ethanol. The crystals were dried fot 3 hours at 60°C under reduced pressure (vacuum pump) to obtain 7.80g (yield: 76.3%) of 4-(2-methyl-l-imidazolyl)-2,2-diphenylbutanamide as white crystalline powder. HPLC purity : 100%. [Industrial applicability] Upon preparing 4-(2-methyl-l-imidazolyl)-2,2- dipheny Ibutanamide, it has become clear that, by separating 4-(2 -methyl-l-imidazolyl)-2,2-diphenylbutanamide as an acid salt of hydrochloride, phosphate or the like, or hydrate thereof, being an intermediate, followed by purification, then, by neutralizing with alkali metal hydroxide and- recrystallizing using alcohol, high -quality 4- (2-methyl-l-imidazolyl) -2,2-diphenyIbutanamide can be provided in good efficiency. According to the invention, industrially advantageous preparative process of 4-(2-methyl-l-imidazolyl)-2,2-diphenylbutanamide has been established, thereby making it possible to provide it as a high-purity and high-quality medicinal drug. SCOPE OF CLAIMS 1. Salt of 4-(2-methyl-l-imidazolyl)-2,2-diphenylbutanamide, or hydrate of that salt. 2. Hydrochloride or phosphate of 4-(2-methyl-l-imidazolyl)-2,2- diphenylbutananu.de, or hydrate of that acid salt. 3. Acid salt or hydrate of that acid salt of Claim 2, characterized in that it is a preparative intermediate of 4- (2-methyl- 1-imidazolyl) -2,2-diphenylbutanainide. 4. Acid salt or hydrate of that acid salt of Claim 2, characterized in that it is a preparative intermediate for preparing purified product of 4-(2-methyl- 1-imidazolyl)-2,2-diphenylbutanamide, and it allows easy separation and purification. 5. A process for preparing separated and purified product of acid sal£ or hydrate of that acid salt of Claim 2, wherein inorganic acid or organic acid is reacted with crude crystals of 4-(2-methyl- 1-imidazolyl) -2,2-diphenylbutanamide to separate and purify the acid salt or hydrate of that acid salt. 6. A process for preparing purified 4-(2-methyl-l-imidazolyl) -2,2-diphenylbutanamide by neutralizing the separated and purified product of acid salt or hydrate of that acid salt of Claim 5 with alkali. 7. A process for preparing purified product of 4-(2-methyl-l- imidazolyl)-2,2-diphenylbutanamide, wherein the acid salt of 4-(2-methyl-1-imidazolyl)-2,2-diphenylbutanamide, or hydrate of that acid salt separated in Claim 5 is recrystallized from alcohol and ethyl acetate, followed by neutralization, by using hydroxide of alkali metal, and the crystals obtained are recrystallized from alcohol or water-containing alcohol. 8. The acid salt or hydrate of that acid salt of Claim2 or 3, that allows to provide 4-(2-methyl-l-imidazolyl)-2,2-diphenylbutanamide with high quality in good yield only by simple recrystallization. Salt of 4-(2-methyl-l-imidazolyl)-2,2-diphenylbutanamide, hydrate substantially as herein described with reference to foregoing description, examples and the accompanying tables. 9. Salt of 4-(2-methyl-I- imidazolyl)-2,2- diphenylbutanamide, or hydrate substantially as herein described with reference to the foregoing description, examples and the accompanying tables. 10. Hydrochloride or phosphate of 4-(2-methyl-1-imidazolyl)-2,2- diphenylbutanamide, or hydrate substantially as herein descril with reference to the foregoing description, examples and the accompanying tables. 11. Acid salt or hydrate substantially as herein described with reference to the foregoing description, examples and the accompanying tables. 12. A process for preparing separated and purified product of acid salt or hydrate substantially as herein described with reference to the foregoing description, examples and the accompanying tables. 13. A process for preparing purified 4-(2-methyl-1-imidazolyl)-2,2- diphenylbutanamide substantially as herein described with reference to the foregoing description, examples and the accompanying tables.

Documents:

4480-DELNP-2007 (F-1&2).pdf

4480-DELNP-2007 (F-13) PKK.pdf

4480-delnp-2007-1-Correspondence Others-(04-10-2013).pdf

4480-delnp-2007-1-GPA-(04-10-2013).pdf

4480-delnp-2007-abstract.pdf

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4480-delnp-2007-Correspondence Others-(04-10-2013).pdf

4480-delnp-2007-Correspondence Others-(08-10-2013).pdf

4480-delnp-2007-Correspondence Others-(21-08-2014).pdf

4480-delnp-2007-Correspondence Others-(25-08-2014).pdf

4480-delnp-2007-Correspondence Others-(29-07-2013).pdf

4480-delnp-2007-Correspondence-Others-(02-12-2008).pdf

4480-delnp-2007-Correspondence-Others-(11-12-2007).pdf

4480-delnp-2007-Correspondence-Others-(22-07-2013).pdf

4480-delnp-2007-correspondence-others.pdf

4480-delnp-2007-description (complete).pdf

4480-delnp-2007-form-1.pdf

4480-delnp-2007-Form-18-(02-12-2008).pdf

4480-delnp-2007-Form-2-(08-10-2013).pdf

4480-delnp-2007-form-2.pdf

4480-delnp-2007-Form-3-(11-12-2007).pdf

4480-delnp-2007-Form-3-(21-08-2014).pdf

4480-delnp-2007-Form-3-(22-07-2013).pdf

4480-delnp-2007-form-3.pdf

4480-delnp-2007-form-5.pdf

4480-delnp-2007-GPA-(21-08-2014).pdf