Title of Invention

PRODUCTION OF GLYCOPROTEINS WITH REDUCED O-GLYCOSYLATION

Abstract A method is described for producing protein compositions having reduced amounts of O-linked glycosylation. The method includes producing the protein in cells cultured in the presence of an inhibitor of Pmt-mediated 0-linked glycosylation and/or in the presence of one or more &agr;-l,2- mannosidases.
Full Text TITLE OF THE INVENTION
PRODUCTION OF GLYCOPROTEINS WITH REDUCED 0-GLYCOSYLATION
BACKGROUND OF THE INVENTION
(1) Field of the Invention
The present invention relates to compositions and methods for producing proteins having specific glycosylation patterns. In particular, the present invention relates to compositions and methods for producing proteins having reduced O-linked glycosylation,
(2) Description of Related Art
Glycoproteins mediate many essential functions in humans and other mammals, including catalysis, signaling, cell-cell communication, and molecular recognition and association. Glycoproteins make up the majority of non-cytosolic proteins in eukaryotic organisms (Lis and Sharon, 1993, Eur. J. Biochem. 218:1-27). Many glycoproteins have been exploited for therapeutic purposes, and during the last two decades, recombinant versions of naturally-occurring glycoproteins have been a major part of the biotechnology industry. Examples of recombinant glycosylated proteins used as therapeutics include erythropoietin (EPO), therapeutic monoclonal antibodies (mAbs), tissue plasminogen activator (tPA), interferon-P (IFN-P), granulocyte-macrophage colony stimulating factor (GM-CSF), and human chorionic gonadotrophin (hCH) (Gumming e^ £7/., 1991, Glycobiology 1:115-130). Variations in glycosylation patterns of recombinantly produced glycoproteins have recently been the topic of much attention in the scientific community as recombinant proteins produced as potential prophylactics and therapeutics approach the clinic.
In general, the glycosylation structures of glycoprotein oligosaccharides will vary depending upon the host species of the cells used to produce them. Therapeutic proteins produced in non-human host cells are likely to contain non-human glycosylation which may elicit an immunogenic response in humans—e.g. hypermannosylatton in yeast (Ballou, 1990, Methods Enzymol. 185:440-470); a(l,3)-fucose and p(l,2>-xylose in plants, (Cabanes-Macheteau et aL, 1999. Glycobiology, 9: 365-372); N-glycolylneuraminic acid in Chinese hamster ovary cells (Noguchi et al,, 1995. J. Biochem. 117: 5-62); and, Gala-l,3Gal glycosylation in mice (Borrebaeck, e/a/.,1993, Immun. Today, 14: 477-479), Carbohydrate chains bound to proteins in animal cells include N-glycoside bond type carbohydrate chains (also called N-glycans; or N4inked glycosylation)' bound to an asparagine (Asn) residue in the protein and 0-glycoside bond type carbohydrate chains (also called O-glycans; or O-linked glycosylation) bound to a serine (Ser) or threonine (Thr) residue in the protein.
Because the oligosaccharide structures of glycoproteins produced by non-human mammalian cells tend to be more closely related to those of human glycoproteins, most commercial glycoproteins are produced in mammalian cells. However, mammalian cells have several important disadvantages as host cells for protein production. Besides being costly, processes for producing

proteins in mammalian cells produce heterogeneous populations of glycoforms, have low volumetric titers, and require both ongoing viral contamment and significant time to generate stable cell lines.
It is well recognized that the particular glycofonns on a protein can profoundly affect the properties of the protein, including its pharmacokinetic, pharmacodynamic, receptor-interaction, and tissue-specific targeting properties (Graddis et al, 2002. Curr Pharm Biotechnol. 3: 285-297). For example, it has been shown that different glycosylation patterns of Igs are associated with different biological properties (Jefferis and Lund, 1997, Antibody Eng. Chem. Immunol, 65: 111-128; Wright and Morrison, 1997, Trends Biotechnol, 15: 26-32). It has further been shown that galactosylation of a glycoprotein can vary with cell culture conditions, which may render some glycoprotein compositions immunogenic depending on the specific galactose pattern on the glycoprotein (Patel et aL^ 1992. Biochem J. 285: 839-845). However, because it is not known which specific glycoform(s) contribute(s) to a desired biological function, the ability to enrich for specific glycoforms on glycoproteins is highly desirable. Because different glycoforms are associated with different biological properties, the ability to enrich for glycoproteins having a specific glycoform can be used to elucidate the relationship between a specific glycoform and a specific biological function of the glycoprotein. Also, the ability to enrich for glycoproteins having a specific glycoform enables the production of therapeutic glycoproteins having particular specificities. Thus, production of glycoprotein compositions tliat are enriched for particular glycoforms is highly desirable.
While the pathway for N-linked glycosylation has been the subject of much analysis, the process and fimction of 0-linked glycosylation is not as well understood. However, it is known that in contrast to A'^linked glycosylation, 0-glycosylation is a posttranslational event, which occurs in ihtcis-Golgi (Varki, 1993, Glycobiol, 3: 97-130). While a consensus acceptor sequence for O-linked glycosylation like that for N-linked glycosylation does not appear to exist, a comparison of amino acid sequences around a large number of O-iinked glycosylation sites of several glycoproteins show an increased fi-equency of proline residues at positions ~1 and +3 relative to the glycosylated residues and a marked increase of serine, threonme, and alanine residues (Wilson et al., 1991, Biochem. J., 275: 529-534). Stretches of serine and threonine residues in glycoproteins, may also be potential sites for O-glycosylation.
One gene family that has a role in O-linked glycosylation are the genes encoding the Dol'P-Man:Protein (Ser/Thr) Mannosyl Transferase (Pmt). These highly conserved genes have been identified in both higher eukaryotes such as humans, rodents, insects, and the like and lower eukaryotes such as fungi and the like. Yeast such as Saccharomyces cerevisiae and Pichia pastor is encode up to seven PA^ genes encoding Pmt homologues (reviewed in Wilier et al, Curr. Opin. Struct. Biol 2003 Oct; 13(5): 621-30.). In yeast, O-linked glycosylation starts by the addition of the initial mannose from dolichol-phosphate mannose to a serine or threonine residue of a nascent glycoprotein in the endoplasmic reticulum by one of the seven 0-mannosyl transferases genes. While there appear to be seven PMT genes encoding Pmt homologues in yeast, O-mannosylation of secreted fiingal and heterologous proteins

in yeast is primarily dependent on the genes encoding Pmtl and Pmt2, which appear to function as a heterodimer, PMTl and PMr2 and their protein products, Pmtl and Pmt2, respectively, appear to be highly conserved among species.
Tanner et aL in U. S. Patent No. 5 J14,377 describes the PA^TJ and PMT2 genes of Saccharomyces cerevisiae and a method for making recombinant proteins having reduced O-linked glycosylation that uses fungal cells in which one or more of PA/T genes have been genetically modified so that recombinant protems are produced, which have reduced O-linked glycosylation.
Ng et al, in U.S, Published Patent Application No. 20020068325 discloses inhibition of O-glycosylation through the use of antisense or cosuppression or through tiie engineering of yeast host strains that have loss of function mutations in genes associated with O-linked glycosylation, in particular, one or more of the PMT genes.
UDP-N-acetyl-alpha-D-galactosamineipolypeptideN-acetylgalactosaminyl-transferases (GalNAc-transferases) are involved in mucin type O-linked glycosylation found in higher eukaryotes. These enzymes hiitiate O-glycosylation of specific serine and threonine amino acids in proteins by adding N-acetylgalactosamine to the hydroxy group of these amino acids to which maimose residues can then be added in a step-wise manner. Clausen et al. in U. S. Patent No. 5,871,990 and U.S. Published Patent Application No. 20050026266 discloses a family of nucleic acids encoding UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetyl galactosaminyl-transferases (GalNAc-transferases). Clausen in U.S. Published Patent Application No. 20030186850 discloses the use of GalNAc-beta-benzyl to selectively inhibit lectms of polypeptide GalNAc-transferases and not serve as substrates for other glycosyltransferases involved in O-glycan biosyntheses, thus inhibiting O-glycosylation.
hihibitors of O-linked glycosylation have been described. For example. Orchard et al in U.S. Patent No. 7,105,554 describes benzylidene thiazolidinediones and their use as antimycotic agents, e.g., antifungal agents. These benzylidene thiazolidinediones are reported to inhibit the Pmtl enzyme, preventing the formation of the O-linked mannoproteins and compromising the integrity of the fungal cell wall. The end result is cell swelling and ultimately death tlirough rupture.
Konrad et aL in U.S. Published Patent Application No. 20020128235 disclose a method for treating or preventing diabetes mellitus by pharmacologically inhibiting O-linked protein glycosylation in a tissue or cell The method relys on treating a diabetic individual with (Z)-l-[N-(3-Ammoniopropyl)-N-(n~propyl)amino] diazen-ium-l,2"diolate or a derivative thereof, which binds O-linked N-acetylglucosamine transferase and thereby inhibits O-linked glycosylation.
Kojima et al. in U. S. Patent No. 5,268,364 disclose therapeutic compositions for inhibition of O-glycosylation using compounds such as benzyle-a-N-acetylgalactosamine, which inhibits extension of O-glycosylation leading to accumulation of 0-a-GalNAc, to block expression of SLex or SLea by leukocytes or tumor cells and thereby inhibit adhesion of these cells to endothelial cells and platelets.

Boime etal, U. S. Patent No. 6,103,501 disclose variants of hormones in which O-linked glycosylation was altered by modifying the amino acid sequence at the site of glycosylation.
The present mventors have found that particular chemical compounds that are mhibitors of Pmt protems, which are generally lethal to fungi, can be used in a way which is not lethal to the host cells for production of recombhiant proteins with reduced 0-linked glycosylation. This enables 0-linked glycosylation of proteins produced jfrom fungi and yeast cells to be controlled. Other classes of chemical compounds, which the inventors believe to be non-lethal inhibitors of the PMT enzymes, are also useful in the production of improved glycoproteins with reduced 0-linked glycosylation. The present inventors have further found that addition to the host cell or cell culture of certain classes of enzymes, namely, a-1,2-mannosidases, alone or in combination with a chemical inhibitor of Pmt proteins effects a further reduction of 0-glycosylation»
BRIEF SUMMARY OF THE INVENTION
The present mvention provides methods for producing proteins and glycoproteins having specific glycosylation patterns. In particular, the present invention provides a method for making a recombinant protein compositions m a host cell in which the 0-linked glycosylation of the recombinant protein is reduced by contacting the host cells with one or more inhibitors of Pmt-mediated 0-linked glycosylation of proteins in the host cell or contacting the host cells or the recombinant protein with one or more a-1,2-mannosidases, or both. The amount of O-linked glycosylation of the recombinant protem or glycoprotein is reduced compared to the amount of O-linked glycosylation of the recombinant protein or glycoprotein produced by the host cell in the absence of the inhibitor,
Pmt-mediated O-linked glycosylation refers to O-linked glycosylation wherein transfer of mannose residues to the serine or threonine residues of a protein is mediated by a protein-O-D-mannosyltransferase (Pmt) or homologue encoded by a PAfTgene or its homologue. The inhibitors of Pmt-mediated O-linked glycosylation include inhibitors that inhibit any one of the homologues of the PMT genes. In a currently preferred aspect, the inhibitor inhibits at least Pmtl and/or Pmt2 activity of fungi and yeast, or the corresponding homologue in other organisms, including but not limited to, mammals, plants, and insects.
Currently, it is preferable that the amount of O-linked glycosylation has been reduced through the use of a chemical inhibitor, for example, a chemical inhibitor encompassed by the class of chemicals called benzylidene thiazolidinediones. In particular embodiments, the chemical inhibitor is selected from the group consisting of 5-[[3,4-bis(phenylmethoxy) phenyl]methylene]-4-oxo-2-thioxo-3-thiazolidineacetic acid; 5-[[3'-(l-Phenylethoxy)"4-(2-phenylethoxy)]phenyl]methylene]'-4-oxo-2-thioxo-3-thiazolidineacetic acid; 3'Hydroxy-4-(2-phenylethoxy)benzaldehyde; 3-(l-Phenylethoxy)-4'-(2-phenylethoxy)-benzaldehyde; 5-[[3-(l-Phenyl-2-hydroxy)ethoxy)-4-(2-ph6nylethoxy)]phenyl]methylene]-4-oxo-2-thioxo-3-thia2olidineaceticacid.

In further aspects, provided is a method for producing recombinant protein compositions having reduced 0-Iinked glycosylation, which use one or more inhibitors of the Pmt proteins involved in 0-linked glycosylation and/or one or more a-l,2-mannosidase enzymes to produce the protein having reduced 0-linked glycosylation. Currently preferred a-l,2-mannosidases may be isolated from eukaryotic cells, including mammalian and yeast cells. In currently preferred embodiments, the a-1,2'-mannosidase is that produced by Trichoderma reeseU Sacchcn'omyces sp., ox Aspergillus sp. In other currently preferred embodiments, the a-l,2-mannosidase may be produced from a chimeric construct comprising a nucleic acid sequence encoding the catalytic domain of an a-l,2"mannosidase operatively linked to a nucleic acid sequence encoding a cellular targeting signal peptide not normally associated with the catalytic domain. In other embodiments, the a"l,2-mannosidase may be separately produced and added to the cell culture, or may be produced by co-expressing the a-l,2-mannosidase with the recombinant glycoprotein.
In particular aspects of the method, the recombinant protein composition comprises a
glycoprotein havmg N-linked glycosylation wherein the recombinant glycoprotein includes at least one
predominant N-glycoform and has reduced 0-linked glycosylation. Therefore, fiirlher provided are
glycoprotein compositions comprising a predominant species of N-glycan structure and having reduced
O-linked glycosylation compared to compositions of the glycoprotein which have been produced in host
cells have not been incubated in the presence of an inhibitor of Pmt-mediated O-linked glycosylation or
an a"l,2-raannosidase capable of trimming more than one mannose residue from a glycans structure. In
pmticular aspects, the glycoprotein composition comprises a glycoprotein having a predominant N-glycan structure selected from the group consisting of Man5GlcNAc2, Man3GlcNAc2,
GlcNAcMan5GlcNAc2, GlcNAcMan3GlcNAc2, GlcNAc2Man3GlcNAc2, GaIGlcNAcMan5GlcNAc2, Gal(GlcNAc)2 Man5GlcNAc2, (GalGIcNAc)2Man5GlcNAc2, NANAGalGlcNAcMan3GlcNAc2, NANA2Gal2GlcNAcMan3GlcNAc2, and GalGlcNAcMan3GlcNAc2 glycoforms. An important aspect of the method is that it provides for a glycoprotein composition comprising reduced 0-Iinked glycosylation and predominantly a specific N-linked glycoform in which the recombinant glycoprotein may exhibit increased biological activity and/or decreased undesired immunogenicity relative to compositions of the same glycoprotein produced from mammalian cell culture, such as CHO cells. An additional advantage of producing the glycoprotein composition comprising reduced 0-linked glycosylation and a predominant N-linked glycoform is that it avoids production of undesired or inactive glycoforms and heterogeneous mixtures, which may induce undesired effects and/or dilute the more effective glycoform. Thus, therapeutic pharmaceutical composition of glycoprotein molecules comprisuig, for example, predominantly Man5GlcNAc2, Man3GlcNAc2, GlcNAcMan5GlcNAc2,
GlcNAcMan3GlcNAc2, GlcNAc2Man3GlcNAc2, GalGlcNAcMan5GlcNAc2, GaI(GlcNAc)2 Man5GlcNAc2, (GalGlcNAc)2Man5GlcNAc2, NANAGalGlcNAcMan3GlcNAc2, NANA2Gal2GlcNAcMan3GlcNAc2, and GalGlcNAcMan3GlcNAc2 glycoforms and having reduced O-linked glycosylation may well be effective at lower doses, thus having higher efficacy/potency.

Therefore, provided is a method of producing a protein having reduced 0-linked glycosylation comprising providing a nucleic acid encoding a protein; introducing the nucleic acid into a host cell to provide a culture of the host cell; contacting the culture with one or more inhibitors of Pmt-mediated 0-linked glycosylation; and isolating tlie glycoprotein produced by the host cell in the presence of the inhibitor to produce the protein having reduced O-linked glycosylation.
In particular aspects of the method, the culture is grown for a time sufficient to provide a multiplicity of the host cells having the nucleic acid before contacting the culture with the one or more inhibitors of Pmt-mediated O-linked glycosylation or the culture is grown in the presence of the one or more inhibitors of Pmt-mediated O-linked glycosylation at the time the culture is established.
In a further aspect of the method, the nucleic acid encoding the protein is operably linked to an inducible promoter. Then the culture is grown for a time sufficient to provide a multiplicity of the host cells having the nucleic acid before contacting the culture with the one or more inhibitors of Pmt-mediated O-linked glycosylation and an inducer of the promoter to induce expression of the protein and isolating the protein produced by the host cell in the presence of the one or more inhibitors and the inducer to produce the protein having reduced O-linked glycosylation or the culture is contacted with an inducer of the promoter to induce expression of the protein for a time before contacting the culture with the one or more inhibitors of Pmt-mediated O-linked glycosylation and isolatmg the protein produced by the host cell in the presence of the inhibitor and the inducer to produce the protein having reduced O-linked glycosylation.
Further provided is a method of producing a protein having reduced O-linked glycosylation comprising providing a nucleic acid encoding a protein; introducing the nucleic acid into a host cell to provide a culture of the host cell; contacting the culture with one or more a"l52-mannosidase enzymes; and isolating the protein produced by the host cell in the presence of the one or more a-1,2-mannosidase enzymes to produce the glycoprotein having reduced O-linked glycosylation.
In particular aspects of the method, the culture is grown for a time sufficient to provide a multiplicity of the host cells having the nucleic acid before contacting the culture with the one or more a-1,2-mannosidase enzymes cosylation. In other aspects, the culture is grown in the presence of the one or more a-l,2"mannosidase enzymes.
In further aspects of the method, a second nucleic acid encoding the one or more a-l,2-mannosidase enzymes is provided and introducing the second nucleic acid into the host cell. In particular aspects, a second nucleic acid encoding the one or more a-l,2*-mannosidase en2ymes operably linked to an mducible promoter is provided and introducing the second nucleic acid into the host cell and the culture is grown for a time sufficient to provide a multiplicity of the host cells before inducing expression of the protein and the one or more a-l,2-mannosidase enzymes to produce the protein having reduced O-linked glycosylation or expression of the protein is induced for a time before inducing expression of the one or more a"l,2-mannosidase enzymes to produce the protein having reduced O-linked glycosylation or

expression of the one or more a-l,2"mannosidase enzymes is induced for a time before inducing expression of the protein to produce tlie protein having reduced O-linked glycosylation.
Further provided is a method of producing a protein having reduced O-linked glycosylation comprising providing a nucleic acid encoding a protein operably linked to an inducible promoter; introducing the nucleic acid into a host cell and growing the host cell containing the nucleic acid to produce a culture of the host cell; contactmg the culture with one or more inhibitors of Pmt-mediated O-linked glycosylation and one or more one or more a-l,2-mannosidase enzymes; and isolating the glycoprotein produced by tlie host cell in the presence of the one or more inhibitors and the one or more one or more a-l,2-mannosidase enzymes to produce the protein having reduced O-linked glycosylation.
In particular aspects of the method, the culture is grown for a time sufficient to provide a multiplicity of the host cells having the nucleic acid before contacting the culture with the one or more inhibitors of Pmt-mediated O-lmked glycosylation or the culture is grown in the presence of the one or more inhibitors of Pmt-mediated O-linked glycosylation at the time the culture is established.
In a fiirther aspect of the method, the nucleic acid encoding the protein is operably linked to an inducible promoter. Then the culture is grown for a time sufficient to provide a multiplicity of the host cells having the nucleic acid before contacting the culture with the one or more inhibitors of Pmt-mediated O-linked glycosylation and an mducer of the promoter to induce expression of the protein and isolating the protein produced by the host cell in the presence of the one or more inhibitors and the inducer to produce the protein having reduced O-linked glycosylation or the culture is contacted with an inducer of the promoter to induce expression of the protein for a tune before contacting the culture with the one or more inhibitors of Pmt-mediated O-linked glycosylation and isolating the protein produced by the host cell in the presence of the inhibitor and the inducer to produce the protein having reduced O-linked glycosylation.
In particular aspects of the method, the culture is grown for a time sufficient to provide a muhiplicity of the host cells having the nucleic acid before contacting the culture with the one or more a-1,2-mannosidase enzymes. In other aspects, the culture is grown in the presence of the one or more a-1,2-mannosidase enzymes.
In further aspects of the method, a second nucleic acid encoding the one or more a-1,2-mannosidase enzymes is provided and introducing the second nucleic acid into the host cell. In particular aspects, a second nucleic acid encoding the one or more a-1,2-mannosidase enzymes operably linked to an inducible promoter is provided and introducing the second nucleic acid into the host cell and the culture is grown for a tune sufificient to provide a multiplicity of the host cells before inducing expression of the protein and the one or more a"l,2'mamiosidase enzymes to produce the protein having reduced 0-linked glycosylation or expression of the protein is induced for a time before inducing expression of the one or more a-1,2-mannosidase enzymes to produce the protein having reduced O-linked glycosylation or

expression of the one or more a-l,2-mannosidase enzymes is induced for a time before inducing expression of the protein to produce the protein having reduced 0-linked glycosylation.
hi further aspects of the above methods that use one or more inhibitors of a Pmt protein, currently, it is preferred that the one or more inhibitors is selected from the class of molecules comprising benzylidene thiazolidinediones. Currently, it is preferable that the one or more inhibitors be selected from the group consisting of 5-'[[3,4-bis(phenyhnethoxy) phenyl]methylene]-4-oxo-2-thioxo-3-thiazolidineaceticAcid;5-[[3-(l-Phenylethoxy)-4-(2-phenylethoxy)]phenyl]methylene]-4-oxo-2-thioxo-' 3-thiazolidineacetic Acid; and 5-[[3-(l-Phenyl-2-hydroxy)ethoxy)-4-(2-phenylethoxy)]phenyl]methylene]-4-oxo-2-ihioxo-3-thiazolidineacetic Acid.
hi particular aspects of the above methods that use an a-l,2-mannosidase, it is currently preferable that the a-l,2-raannosidase is selected from the group consisting of Trichoderma reesei, Saccharomyces sp., and Aspergillus sp. Currently, it is preferable that the a-l,2-mannosidase is from ■ Trichoderma reesei. Alternatively, the host cell can include in addition to the first nucleic acid encodmg the protein or glycoprotein, a second nucleic acid, which encodes the a-l,2-mannosidase, operably linked to an inducible promoter. Expression of the a"l,2'mannosidase and the protein or glycoprotein can be induced simultaneously or expression of the protem or glycoprotein induced before expression of the a-1,2-mannosidase or vice versa.
While the method can be performed using any host cell that produced proteins having O-linked glycosylation, in currently preferred aspects, the host cell is a lower eukaryotic cell, preferably a fungal cell or a yeast cell Currently, it is preferred that the host cell be selected from the group consisting of cells from K iactis, Pichia pastoris, Pichia methanoUca, and Hansenula, hi further embodiments for producing recombinant glycoproteins in particular, the host cell is a yeast or filamentous fungal cell that has been genetically modified to produce glycoproteins with predominantly a particular N-glycan structure, hi particularly preferred aspects, the host cells are genetically modified so that they express recombinant glycoproteins in which the glycosylation pattern is human-like or humanized, hi particular, the host cells can be modified so that they express recombinant glycoprotehis having predominantly a particular deshed N-glycan structure. A lower eukaryotic host cell when used herein in connection with glycosylation profiles, refers to any eukaryotic cell which ordinarily produces high maimose containing N-linked glycans, and thus, includes most typical lower eukaryotic cells, including uni- and multi-cellular fungal and algal cells.
All publications, patents, patent applications, and other references mentioned herein are hereby incorporated by reference in their entireties.
BRIEF DESCRIPTION OF THE DRAWINGS
Figure 1 illustrates the effect of Pmt inhibitors on 0-glycosylation of secreted recombinant reporter proteins in Pichia pastoris. The chemical inhibitors of Pmt reduced O-glycosylation to a level similar to that observed in a strain lacking PMTL Western blotting using an anti-

polyhistidine antibody was used to detect His-tagged human Kringle 1-3 domain (Kl-3) of human plasminogen in the growth media of wild-type (lanes 1-3) and pmtl (lanes 4-5) strains. The slower migrating bands (seen as a higher molecular weight smear for Kl-3 in lane 1) indicate 0-glycosylated protein. Pmti-1, PMT inhibitor 1.
Figure 2 shows a Western blot that demonstrates the effect of 7^ reesei a-raannosidase and the chemical inhibitor Pmti-2 on the 0-glycosylation of immunoglobulin light and heavy chain polypeptides. Both T. reesei a-mannosidase and chemical Pmt inhibitors reduced the level of O-glycosylation,
DETAILED DESCRIPTION OF THE INVENTION
The present invention provides a method for expressing a recombinant protein (includes polypeptides and glycoproteins), which is susceptible to 0-linked glycosylation in a particular host cell, having a reduced amount of 0-linked glycosylation (including no O-linked glycosylation) in that cell type. The method involves inducing expression of a protein of interest in a host cell in which the protein is susceptible to 0-linked glycosylation in the host cell in the presence of a chemical inhibitor of the activity of one or more of the Dol-P-Man:Protein (Ser/Thr) Mannosyl Transferase (Pmt) proteins involved in the transfer of mannose to a serine or threonine residue of the protein in the cell or one or more a 1,2-mannosidases, or both, at the time expression of the protein is induced. The protein that is expressed in the presence of the inhibitor or the one or more a 1,2-mannosidases has a reduced amount of O-linked glycosylation compared to the amount of O-linked glycosylation that would have been present on the protein if it had been produced in the absence of the inhibitor or the one or more a 1,2-mannosidases, or both. Tlie method is particularly useful because it provides a means for producing therapeutically relevant proteins where it is desired that the protein have a reduced amount of O-glycosylation in host cells such as lower eukaryotes, for example yeast, and bacteria, which would normally produce proteins with 04inked glycans, having a reduced number of O-linked glycans. However, while the method is especially suitable for expressing proteins with reduced O-linked glycosylation in lower eukaryotic organisms, the method can also be practiced in higher eukaryotic organisms and bacteria.
The method is an improvement over prior art methods for producing proteins having reduced O-linked glycosylation in host cells in which the proteins are susceptible to O-linked glycosylation. For example. Tanner et al in U, S, Patent No. 5,714,377 describes a method for making recombinant proteins having reduced O-linked glycosylation using fungal cells such as yeast cells in which one or more of jPiWT genes encoding the Pmt protein have been genetically modified so that recombinant proteins are produced, which have reduced O-linked glycosylation. While deletion of either the PMTl ox PMT2 genes in a fungal host cell enables production of a recombinant protein having reduced O-linked glycosylation in the fungal host cell, expression of the PMTI and PMT2 genes are important for host cells growth and either deletion alone also adversely affects the ability of the fungal

host cell to grow thus making it difficult to produce a sufficient quantity of host cells or recombinant protein with a reduced amount of O-linked glycosylation. Deletion of both genes appears to be lethal to the fiingal host cell. Therefore, genetic elimination of the PMTl and PMTl genes in a host cell would appear to be an undesirable means for producing recombinant proteins having reduced O-linked glycosylation.
hi contrast, the PMT genes in the host cells used in the method of the present invention have not been modified or deleted, which enables the host cell to O-glycosylate those proteins that are important for cell growth until which time the activity of the Pmt proteins is inhibited, hi general, this enables the host cells to be grown to higher levels than the levels that could be obtamed if the PMT genes had been deleted. In addition, in particular embodiments, expression of Ihe recombinant protein in the host cell is controlled by an inducible promoter and the Pmt activity in the host cell is not inhibited or one or more a 1,2-mannosidases added, or both, until expression of the recombinant protein is induced. This enables large quantities of host cells containing a nucleic acid encoding a recombinant protein to be produced in culture before inducing expression of the recombinant protein and adding the Pmt inhibitor and/or one or more a 1,2-mannosidases. This can enable production of larger amounts recombinant protein having reduced O-linked glycosylation to be produced in the culture in a shorter period of time than would occur for host cells which have had one or more PMT genes deleted and grow poorly in culture.
This improvement over the prior art also facilitates the production of glycoproteins having reduced O-linked glycosylation in host cells that have been genetically modified to produce glycoprotems having predominantly a particular N-linked glycan structure but which also 0-glycosylate the glycoprotein. Methods for producing a wide variety of glycoproteins having predominantly particular N-linked glycoforms have been disclosed in U.S. Patent No. 7,029,872 and U.S. Published Application Nos, 20050170452,20050260729,20040230042, 20050208617, 20050208617,20040171826, 20060160179, 20060040353, and 20060211085. Any one of the host cells described m the aforementioned patent and patent applications can be used to produce a glycoprotein having predominantly a particular N-linked glycan structure and having reduced 0-Iinked glycosylation using the method disclosed herein. It has been found that some host cells that have been genetically modified to produce glycoproteins having predominantly a particular N-linked glycan structure can grow less well in culture under particular conditions than host cells that have not been modified. For example, particular fungal and yeast cells in which genes involved in hypermannosylation have been deleted and other genes needed to produce particular mammalian or human like N-linked glycan structures have been added, can grow less well than fungal or yeast cells that do not the genetic modifications. In some of these genetically modified fungal or yeast cells, further introducing deletions of the PMTl or PMTl genes either is lethal to the cells or adversely affects the ability of the cells to grow to sufficient quantities in culture. The method herein avoids the potential deleterious effects of deleting the PMTl and PMT2 genes by allowing the cells to grow to sufficient quantities in culture before inducing

expression of the recombinant glycoprotein and adding an inhibitor of the activity of the Pmt proteins, or
one or more a 1,2-mannosidases, or both, to produce the recombinant glycoprotein having predominantly
particular N-linked glycan structures and reduced O-linked glycosylation.
Therefore, an important aspect of the method is that it provides for a glycoprotein
composition comprising reduced 0-Unked glycosylation and a predominantly a specific N-linked
glycoform in which the recombinant glycoprotein may exhibit increased biological activity and/or
decreased undesired immunogonicity relative to compositions of the same glycoprotein produced from
mammalian cell culture, such as CHO cells. An additional advantage of producing the glycoprotein
composition comprising reduced 0-Iinked glycosylation and a predominant N-linked glycoform is that it
avoids production of undesired or inactive glycoforms and heterogeneous mixtures, which may induce
undesired effects and/or dilute the more effective glycoform. Thus, therapeutic pharmaceutical composition of glycoprotein molecules comprising, for example, predominantly Man5GlcNAc2,
Man3GlcNAc2, GIcNAcMan5GlcNAc2, GlcNAcMan3GlcNAc2, GlcNAc2Man3GlcNAc2, GalGlcNAcMan5GlcNAc2, Gal(GlcNAc)2 Man5GlcNAc2, (GalGlcNAc)2Man5GlcNAc2, NANAGalGlcNAcMan3GlcNAc2, NANA2Gal2GlcNAcMan3GIcNAc2, and GalGlcNAcMan3GlcNAc2 glycoforms and having reduced O-Iinked glycosylation may well be effective at lower doses, thios having higher efficacy/potency.
In general, the method for producing proteins having reduced O-linked glycosylation comprises transforming a host cell with a nucleic acid encoding a recombinant or heterologous protein in which it is desirable to produce the protein having reduced O-linked glycosylation. The nucleic acid encoding the recombinant protein is operably linked to regulatory sequences that allow expression of the recombinant protein. Such regulatory sequences include an inducible promoter and optionally an enhancer upstream, or 5', to the nucleic acid encoding the fusion protein and a transcription termination site 3' or down stream from the nucleic acid encoding the recombinant protein. The nucleic acid also typically encodes a 5* UTR region having a ribosome binding site and a 3^ untranslated region. The nucleic acid is often a component of a vector replicable m cells in which the recombinant protein is expressed. The vector can also contain a marker to allow recognition of transformed cells. However, some cell types, particularly yeast, can be successfully transformed with a nucleic acid lacking extraneous vector sequences.
Nucleic acids encoding desired recombinant proteins can be obtained from several sources, cDNA sequences can be amplified from cell lines known to express the protein using primers to conserved regions (see, for example, Marks et aL^ J. Mol. BioL 581-596 (1991)). Nucleic acids can also be synthesized de novo based on sequences in the scientific literature. Nucleic acids can also be synthesized by extension of overlapping oligonucleotides spanning a desu-ed sequence (see, e^g.^ Caldas et al, Protein Engineering, 13, 353-360 (2000)).
In one aspect, the nucleic acid encoding the protein is operably linked to an inducible promoter, which allows expression of the protein to be induced when desired, hi another aspect, the

nucleic acid encoding the protein is operably linked to a constitutive promoter. To facilitate isolation of the expressed protein, it is currently preferable that the protein include a signal sequence that directs the protein to be excreted into the cell culture medium where it can then be isolated. In the first aspect, the transformed host cells are cultured for a time sufficient to produce a desired multiplicity of host cells sufficient to produce the desired amount of protein before adding one or more inhibitors of Pmt-mediated 0-linked glycosylation to the culture medium. The inducer and inhibitor can be added to the culture sunultaneously or the inducer is added to the culture before adding the one or more Pmt inhibitors or the one or more Pmt inhibitors is added to the culture before addmg the inducer. The induced protein is produced having reduced 0-linked glycosylation and can be recovered from the culture medium or for proteins not having a signal sequence, from the host cell by lysis. In the second aspect, wherein the nucleic acid encoding the protein is operably linked to a constitutive promoter, the one or more inhibitors of Pmt-mediated 0-linked glycosylation is added to the culture medium at tlie same time the culture is established and the protein, which is produced having reduced 0-linked glycosylation, can be recovered from the culture medium or for proteins not having a signal sequence, from the host cell by lysis. An example iUustrating the method using an inducible pTomoter is shown in Example 2 and an example illustrating the method using a constitutive promoter is shown in Example 3.
Inhibitors useftil for producing proteins with reduced 0-linked glycosylation are chemicals or compositions that inhibit the activity one or more of the Pmt proteins. When the host cell is a lower eukaryote such as fungi or yeast, it is desirable that the inhibitor inhibit at least the activity of Pmtl or Pmt2, or both. In higher eukaryotes, it is desirable that the inhibitor inhibit activity of tlie homologue in the higher eukaryote that corresponds to the Pmtl or Pmt2, Chemical mhibitors that can be used include the benzylidene thiazolidinediones identified in U.S. Patent No. 7,105,554, which includes 5-[[3,4-bis(phenylmethoxy) phenyl]methylene]-4-oxo-2"thioxo-3-thiazolidineacetic acid; 5-[[3-(l-phenylethoxy)'-4-(2-phenylethoxy)]phenyl]methylene]'4-oxo-2-thioxo-3-thiazolidineaceticacid; 3-hydroxy-4-(2-phenylethoxy)ben2aldehyde; 3-(l-phenylethoxy)-4-'(2-phenylethoxy)-benzaldehyde; and, 5-[[3-(l-phenyl"2-hydroxy)ethoxy)-4-(2-phenylethoxy)]phenyl]methylene]-4-oxo-2-thioxo-3-thiazolidiueacetic acid. Other compounds tlmt might be useftil are the structurally similar compounds disclosed in Voss et ah in WO 94/29287, which discloses methods of makmg arylidene-'4-oxo-2-thioxo-3-thia2olidine carboxylic acids and which are disclosed to be useful in the prophylaxis and treatment of late effects of diabetes as well as the prophylaxis and treatment of atherosclerosis and arteriosclerosis and in Esswein et al. in U.S. Patent No. 65673,816, which discloses methods of making derivatives of rhodaninecarboxylic acids and their use for treatment of metabolic bone disorders.
La the examples, chemical inhibitors selected from the group consisting of 5"[[3,4-bis(phenyknethoxy) phenyl]methylene]-4-oxo-2"thioxo-3-thiazolidineacetic acid; 5'-[[3-(l" phenylethoxy)-4-(2-phenylethoxy)]phenyl]methylene]-4'Oxo-2-thioxo-3-thiazolidineacetic acid; and, 5-[[3~(l-phenyl-2-hydroxy)ethoxy)-4-(2-'phenylethoxy)]phenyI]methylene]'4'-oxo-2'thioxo'>3-thiazolidineacetic acid are shown to be effective in producing recombinant proteins having reduced O-

linked glycosylation in Pichia pastoris strains that had intact, fimctional PMTl and PMr2 genes. Table
1 of Example 2 shows that any one of the above three Pmt chemical mhibitors added to a culture of
recombinant Pichia pastoris having mtact, functional PMT} and PhdT2 genes and transformed with a
nucleic acid encodmg a recombinant, secretable Kringle 1-3 protein operably Imked to an inducible
promoter at the time expression of the recombinant protein was induced, produced a recombinant protein
having a level of reduced O-linked glycosylation that was comparable to the level of O-linked
glycosylation seen for Pichia pastoris cells containing a deletion of either the PMTl or PMT2 gene. The
above Pmti inhibitors have been used in amounts from about 0.03 ^M to 20 fiM to produce proteins
having reduced O-linked glycosylation compared to the amount of O-linked glycosylation on the protem
when grown in similar host cell cultures in the absence of the Pmti inhibitors. The results shown in
Example 3 further shows that the host cell cultures can.be grown in the presence of Pmti inhibitor at an
amount that is sufficient to inhibit O-linked glycosylation without killing thehost cells.
The method can include adding to the culture medium contaming the one or more Pmt
inhibitors one or more a-l52-mannosidase enzymes to produce the recombinant protein having reduced
O-linked glycosylation. The a-l,2-mannosidases are a conserved family of eukaryotic enzymes for maturation of N-glycans, which are capable of trimming Man9GlcNAc2 to MangGlcNAca in yeast.
(Vallee et al, 2000, EMBO J., 19: 581-588). The a-l,2-raannosidases are also known as class I a-
mannosidases and have been identified in manunalian, lower eukaryotic species, and insect cells (Kawar
et al., 2000, Glycobioiogy 10: 347-355). Mammalian cells are known to have several class I a-
mannosidases, some of which are capable of trimming multiple mannose residues (Moremen et al, 1994,
Glycobioiogy 4: i 13-125), while yeast appear to have fewer, more specialized a-l,2-mannosidases. For
example, Saccharomyces has been disclosed to have a single a-l,2-raannosidase encoded by MNNl, which removes one specific mannose residue (for example, Man9GlcNAc2 to Man8GlcNAc2)
(Herscovics, 1999, Biochim Biophys Acta., 1473: 96-107). Thus, the endogenous a-l,2-maimosidase present in many lower eukaiyotes such as fungi and yeast and which cannot remove multiple mannose residues from Glycan structures, is not capable of enabling production of proteins having reduced O-linked glycosylation. Therefore, the method herein requires introduction into the culture medium containing the host cells an a-l,2-maxmosidase capable of trimming multiple mannose residues from an O-linked Glycan or introduction into the host cell a nucleic acid encoding an a-l,2-manuosidase capable of trimming multiple mannose residues from an O-linked Glycan. The a-i,2-mannosidase herein includes the intact, native a-l,2-mannosidase; an a-'l,2-mannosidase modified to enhance its a-1,2-mannosidase activity; an a-l,2'-mannosidase modified to decrease its a-lj2'mannosidase activity; and, a recombinant a-l,2-mannosidase comprising at least the catalytic domain having the a-l,2-mannosidase activity (for example, a fusion protein comprising the catalytic domain havmg the a-lj2-mannosidase activity fiised to heterologous proteins, polypeptides, or peptides).
ha particular embodiments, the a-l,2-mannosidase, which is capable of trimming multiple mannose residues from an O-linked glycans and is added to the cell culture, is produced by

Trichoderma sp., Saccharomyces sp., ov Aspergillus sp. Currently, preferred a-l,2'maimosidases are obtained from Trichoderma reesei, Aspergillus niger, ox Aspergillus oryxae. T, reesei is also known as Hypocreajecorina. In Example 3, a transformed yeast comprising an expression cassette, which expresses a recombinant a-l,2-mannosidases comprising the Trichoderma reesei a-l,2-mannosidase catalytic domain fixsed to the Saccharomyces cerevisiea oMAT pre signal sequence, was used to produce recombinant proteins having reduced O-linked giycosylation. Another example of a recombinant a-1,2-mannosidase that could be used in the method herein to produce proteins having reduced 0-lmked giycosylation is the recombinant Trichoderma reesei a -1,2-mannosidase disclosed in Maras et aL, 2000, J, Biotechnol 77:255-263 wherein the Trichoderma reesei a -1,2-maimosidase catalytic domain was fused to a Saccharomyces cerevisiea a-MAT prepro-signal peptide.
The a-1,2-mannosidase can also be produced from a chimeric nucleic acid comprising a nucleic acid sequence encoding at least the catalytic domain of an a-ly2-mannosidase> which is capable of trimming multiple mannose residues from an O-linked glycans, operatively linked to a nucleic acid sequence encoding a cellular targeting signal peptide not normally associated with the catalytic domain. The chimeric nucleic acid can be operably linked to a constitutive or inducible promoter. The chimeric nucleic acid is transformed into a host cell to produce the a-1,2-mannosidase, which is then isolated and then added to the cell culture medium containing cells transformed with the nucleic acid encoding the heterologous protein at the time expression of the protein is induced. Alternatively, the host cell is transformed with the chimeric nucleic acid encoding the a-1,2-mannosidase and the nucleic acid encoding the recombinant protein and co-expressing the a-1,2-mannosidase and the recombinant protein at the same time, hi particular embodiments, both the chimeric nucleic acid encoding the a-1,2-mannosidase and the nucleic acid encoding the recombinant protein as both operably linked to an inducible promoter. In other embodunents, one or both of the promoters are constitutive. Example 3 illustrates the method wherein nucleic acids encoding both the a-mannosidase and the recombinant protein are operably linked to a constitutive promoter, introduced into a host ceil, and a culture of the host cells is then incubated in the presence of one or more Pmt inhibitors to produce the recombinant protein having reduced O-linked giycosylation. Example 3 further shows that there appears that the Pmti inhibitor and the a-1,2-mannosidase appear to synergistically reduce the amount of O-linked giycosylation compared to the amount of O-linked giycosylation m the presence of either alone.
In particular aspects, reduced 0-Iinked giycosylation can be effected by adding only the one or more a-l,2-mannosidases and not one or more Pmt inhibitors to the culture medium. In one aspect, the nucleic acid encoding the recombinant protein is operably linked to an inducible promoter, which allows expression of the recombinant protein to be uiduced when desired. In another aspect, the nucleic acid encoding the protein is operably linked to a constitutive promoter. To facilitate isolation of the expressed recombinant protein, it is currently preferable that the protein include a signal sequence that directs the recombinant protein to be excreted into the cell culture medium where it can then be isolated.

In the first aspect, the transformed host cells are cultured for a time sufficient to produce a desked multiplicity of host cells sufficient to produce the desired amount of the recombinant protein before adding the one or more a-l,2-maimosidases to the culture medium. Tlie mducer and the one or more a-l,2-mannosidases can be added to the culture simultaneously or the inducer is added to the culture before adding the on& or more a-l,2-mannosidases or the one or more a-l,2-mannosidases is added to the culture before adding the inducer. The induced recombinant protein is produced having reduced 0-linked glycosylation and can be recovered from the culture medium or for proteins not having a signal sequence, from the host cell by lysis.
In the second aspect, wherein the nucleic acid encoding tlie recombinant protein is operably linked to a constitutive promoter, tlie one or more a-l,2-mannosidases is added to the culture medium at the same time the culture is established and the recombinant protein, which is produced having reduced O-linked glycosylation, can be recovered from the culture medium or for recombinant proteins not having a signal sequence, from the host cell by lysis.
In a further still aspect for producing proteins having reduced O-linked glycosylation without using an inhibitor of Pmt-mediated 0-linked glycosylation, the host cell is transformed with a chimeric nucleic acid encoding the a-l,2-mannosidase and a nucleic acid encoding the recombinant protein and co-expressing the a-l,2-mannosidase and the recombinant protein to produce the recombinant protein having reduced 0-linked glycosylation. In particular embodiments, both the chimeric nucleic acid encoding the a-l,2-mannosidase and the nucleic acid encoding the recombinant protein as both operably linked to an inducible promoter. In other embodiments, one or both of the promoters are constitutive. In the case of an inducible promoter, the host cells are grown to produce a desired muUiplicity of host cells before inducing expression of the a"l,2-mannosidase and/or recombinant protein. Example 3 illustrates the method wherein nucleic acids encoding both the a-1,2-mannosidases and the recombinant protein are operably linked to a constitutive promoter are introduced into a host cell and a culture of the host cells is then incubated for a time to produce the recombinant protein, which has reduced 0-linked glycosylation compared to the recombinant protein produced in cells in the absence of the a-l,2~mannosidase.
n. Host Cells
While host cells for the method herein includes both higher eukaryote cells and lower eukaryote cells, lower eukaryote cells, for example filamentous fungi or yeast cells, are currently preferred for expression of proteins because they can be economically cultured, give high yields of protein, and when appropriately modified are capable of producing proteins having suitable glycosylation patterns. Lower eukaryotes include yeast, fungi, collar-flagellates, microsporidia, alveolates (e.g., dhioflagellates), stramenopiles (e,g, brown algae, protozoa), rhodophyta (e.g., red algae), plants (e.g., green algae, plant cells, moss) and other protists, Yeast and fangi include, but are not limited to: Pichia sp. (for example, Pichia pastoris, Pichiafinlandica^ Pichia trehalophila^ Pichia koclamae^ Pichia

membranaefacienSy Pichia minuta (Ogataea mifiuta, Pichia lindneri\ Pichia opuntiae, Pichia thermotolerans, Pichia salictaria^ Pichia guercuum> Pichia pijperi, Pichia stiptis, Pichia methanolica), Saccharoffiyces sp. (for example Saccharomyces cerevisiea\ Hansenulapolymorpha, Kluyveromyces sp. (for example, Kluyveromyces lactis), Candida albicans, Aspergillus sp (for example, Aspergillus nidulans, Aspergillus nigen Aspergillus oryzae), Trichoderma reesei, Chysospormm lucknowense, Fusarium sp. (for example, Fusarium gramineum, Fusarium venenatum)^ Physcomiti'ellapatens and Neurospora crassa. Yeast, in particular, are currently preferred because yeast offers established genetics allowing for rapid transformations, tested protein localization strategies, and facile gene knock-out techniques. Suitable vectors have expression control sequences, such as promoters, including 3-phosphoglycerate kinase or other glycolytic enzymes, and an origin of replication, termination sequences, and the like as desired.
Various yeasts, such as K lactis, Pichia pastoris^ Pichia methanolica, and Hansenula polymorpha are currently preferred for cell culture because they are able to grow to high cell densities and secrete large quantities of recombinant protein. Likewise, filamentous fungi, such as Aspergillus niger, Fusarium sp, Neurospora crass, and others can be used to produce recombinant proteins at an industrial scale.
Lower eukaryotes, in particular filamentous fungi and yeasty can be genetically modified so that they express proteins or glycoproteins in which the glycosylation pattern is human-like or humanized. This can be achieved by eliminating selected endogenous glycosylation enzymes and/or supplying exogenous enzymes as described by Gemgross et ah in U.S. Patent No. US7029872, and U. S. Published Patent Application Nos. 20040018590, 20050170452, 20050260729, 20040230042, 20050208617,20040171826,20050208617, 20060160179, 20060040353, and 20060211085. Thus, a host cell can additionally or alternatively be engineered to express one or more enzymes or enzyme activities, which enable the production of particular N-glycan structures at a high yield. Such an ensyme can be targeted to a host subcellular organelle in which the enzyme will have optimal activity, for example, by means of signal peptide not normally associated with the enz^mQ. Host cells can also be modified to express a sugar nucleotide transporter and/or a nucleotide diphosphatase enzyme. The transporter and diphosphatase improve the efficiency of engineered glycosylation steps, by providing the appropriate substrates for the glycosylation enzymes in the appropriate compartments, reducing competitive product inhibition, and promoting the removal of nucleoside diphosphates. See, for example, Gemgross et al, in U.S. Published Patent Application No,20040018590 and Hamilton, 2003, Science 301: 1244-46 and the aforementioned U.S. patent and patent applications.
By way of example, a host cell (for example, yeast or fungal) can be selected or engineered to be depleted in 1,6-mannosyl transferase activities, which would otherwise add mannose residues onto the N-glycan of a glycoprotein, and to further include a nucleic acid for ectopic expression of an a-1,2 mannosidase activity, which enables production of recombinant glycoproteins having greater than 30 mole percent Man5GlcNAc2 N-glycans. When a glycoprotein is produced in the host cells

according to the method described herein, the host cells will produce a glycoprotein having predominantly a Man5GicNAc2 N-glycan structure and reduced 0-glycosylation compared to the
glycoprotein produced m the cell otherwise. In a further aspect, the host cell is engineered to further mclude a nucleic acid for ectopic expression of GlcNAc transferase I activity, which enables production of glycoproteins having predominantly GlcNAcMan5GlcNAc2 N-glycans, When a glycoprotein is produced in the host cells according to the method described herein, the host cells will produce a glycoprotein having predominantly a GlcNAcMan5GlcNAc2 N-glycan structure and reduced O-
glycosylation compared to the glycoprotein produced in the cell otherwise. In a further still aspect, the host cell is engineered to further include a nucleic acid for ectopic expression of mannosidase II activity, which enables production of glycoproteins having predominantly GlcNAcMan3GlcNAc2 N-glycans.
When a glycoprotein is produced in the host cells according to the method described herein, the host cells will produce a glycoprotein having predominantly a GlcNAcMan3GlcNAc2 N-glycan structure and
reduced 0-glycosylation compared to the glycoprotem produced m the cell otherwise. In a further still aspect, the host cell is engineered to fiirther include a nucleic acid for ectopic expression of GlcNAc transferase II activity, which enables production of glycoproteins having predominantly GlcNAc2Man3GlcNAc2 N-glycans. When a glycoprotein is produced in the host cells according to the
method described herein, the host cells will produce a glycoprotem having predominantly a GlcNAc2Man3GlcNAc2 N-glycan structure and reduced 0-glycosylation compared to the glycoprotein
produced in the cell otherwise. In further still aspects, the above host cells can be further engineered to produce particular hybrid or complex N-glycan or human-like N-glycan structures by further including one or more higher eukaryote genes involved in N-linked glycosylation, in any combination, that encode for example, sialytransferase activities, class n and IH mamiosidase activities, GlcNAc transferase H, HI, IV, V, VI, DC activity, and galactose transferase activity. It is currently preferable that the cells further include one or more of nucleic acids encoding UDP-specific diphosphatase activity, GDP-specific diphosphatase activity, and UDP-GlcNAc transporter activity.
Plants and plant cell cultures may be used for expression of proteins and glycoproteins with reduced 0-linked glycosylation as taught herein (See, for example, Larrick & Fry, 1991, Hum. Antibodies Hybridomas 2: 172-89); Benvenuto et al, 1991, Plant Mol. Biol. 17: 865-74); Darin et aL, 1990, Plant Mol. Biol. 15: 281-93); Hiatt et at., 1989, Nature 342: 76-8). Preferable plant hosts include, for example, Arabidopsis^ Nicotiana tabaciim, Nicotiasia rustica, and Solanum tuberosum.
Insect cell culture can also be used to produce proteins and glycoproteins proteins and glycoproteins with reduced 0-linked glycosylation, as taught herein for example, baculovirus-based expression systems {See, for example, Putlitz etal, 1990, Bio/Technology 8: 651-654).
Although not currently as economical to culture as lower eukaryotes and prokaryotes, mammalian ils$nQ cell culture can also be used to express and produce proteins and glycoproteins with reduced 0-linked glycosylation as taught herein {See Winnacker, From Genes to Clones (VCH Publishers, NY, 1987). Suitable hosts include CHO cell lines, various COS cell lines, HeLa cells.

preferably myeloma cell lines or the like or transformed B-cells or hybridomas. Expression vectors for these cells can include expression control sequences, such as an origin of replication, a promoter, an enhancer (Queen et al., 19861, nununol Rev. 89:49-68), and necessary processing information sites, such as ribosome binding sites, RNA splice sites, polyadenylation sites, and transcriptional terminator sequences. Expression control sequences are promoters derived from immunoglobulin genes, SV40, Adenovirus, bovine Papilloma Virus, cytomegalovirus and the like. Generally, a selectable marker, such as a neoR expression cassette, is included in the expression vector.
The nucleic acid encoding the protein to be expressed can be transferred into the host cell by conventional methods, which vary depending on the type of cellular host. For example, calcium phosphate treatment, protoplast fusion, natural breeding, lipofection, biolistics, viral-based transduction, or electroporation can be used for cellular hosts. Tungsten particle ballistic transgenesis is preferred for plant cells and tissues. (See, generally, Maniatis et al. Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Press, 1982))
Once expressed, the proteins or glycoproteins having reduced 0-linked glycosylation can be purified according to standard procedures of the art, including ammonium sulfate precipitation, affinity columns, column chromatography, gel electrophoresis and the like {See, generally, Scopes, R., Protein Purification (Springer-Verlag, N.Y., 1982)). Substantially pure glycoproteins of at least about 90 to 95% homogeneity are preferred, and 98 to 99% or more homogeneity most preferred, for pharmaceutical uses. Once purified, partially or to homogeneity as desired, the proteins can then be used therapeutically (including extracorporeally) or in developing and performing assay procedures, inununofluorescent stainings, and the like. {See^ generally, Immunological Methods, Vols. I and n (Lefkovits and Pemis, eds.. Academic Press, NY, 1979 and 1981).
Therefore, fiirther provided are glycoprotein compositions comprising a predominant species of N-glycan structure and having reduced O-linked glycosylation compared to compositions of the glycoprotein which have been produced in host cells have not been incubated in the presence of an inhibitor of Pmt-mediated O-linked glycosylation or an a-l,2-mannosidase capable of trimming more than one mannose residue from a glycans structure or both. In particular aspects, the glycoprotein composition comprises a glycoprotein having a predominant N-glycan structure selected from the group consisting of Man5GloNAc2, Man3GlcNAc2, GlcNAcMan5GlcNAc2, GlcNAcMan3GlcNAc2, GlcNAc2Man3GlcNAc2, GalGlcNAcMan5GlcNAc2, Gal(GlcNAc)2 Man5GIcNAc2, (GalGlcNAc)2Man5GlcNAc2,NANAGalGlcNAcMan3GlcNAc2,NANA2Gal2GlcNAcMan3GlcNAc2, and GalGlcNAcMan3GlcNAc2 glycoforms,
m. Pharmaceutical Compositions
Proteins and glycoproteins having reduced 0-Iinked glycosylation can be incorporated into pharmaceutical compositions comprising the glycoprotein as an active therapeutic agent and a variety of other pharmaceutically acceptable components {See, Remington's Pharmaceutical Science

(15th ed.. Mack Publishing Company, Easton. Pennsylvania, 1980). The preferred form depends on the intended mode of administration and therapeutic application. The compositions can also include, depending on the formulation desired, pharraaceutically-acceptable, non-toxic carriers or diluents, which are defined as vehicles commonly used to formulate pharmaceutical compositions for animal or human administration. The diluent is selected so as not to affect the biological activity of the combination. Examples of such diluents are distilled water, physiological phosphate-buffered saline, Ringer's solutions, dextrose solution, and Hank's solution. In addition, the pharmaceutical composition or formulation can also include other carriers, adjuvants, or nontoxic, nontfaerapeutic, nonimmunogenic stabilizers, and the like.
Pharmaceutical compositions for parenteral administration are sterile, substantially isotonic, pyrogen-free and prepared in accordance witli GMP of the FDA or similar body. Glycoproteins can be administered as injectable dosages of a solution or suspension of the substance in a physiologically acceptable diluent with a pharmaceutical carrier that can be a sterile liquid such as water oils, saline, glycerol, or ethanol. Additionally, auxiliary substances, such as wetting or emulsifying agents, surfactants, pH buffering substances and the like can be present in compositions. Other components of pharmaceutical compositions are those of petroleum, animal, vegetable, or synthetic origin, for example, peanut oil, soybean oil, and mineral oil. In general, glycols such as propylene glycol or polyethylene glycol are preferred liquid carriers, particularly for injectable solutions. Glycoproteins can be administered in the form of a depot injection or implant preparation which can be formulated in such a maimer as to permit a sustained release of the active ingredient. Typically, compositions are prepared as injectables, either as liquid solutions or suspensions; solid fonns suitable for solution in, or suspension in, liquid vehicles prior to injection can also be prepared. The preparation also can be emulsified or encapsulated in liposomes or micro particles such as polylactide, polyglycolide, or copolymer for enhanced adjuvant effect, as discussed above {See Langer, Science 249, 1527 (1990) and Hanes, Advanced Drug Delivery Reviews 28, 97^119 (1997).
Unless otherwise defined herein, scientific and technical terms and phrases used in connection with the present invention shall have the meanings that are commonly understood by those of ordinary skill in the art. Further, unless otherwise required by context, singular terms shall include the plural and plural terms shall include the singular. Generally, nomenclatures used in connection with, and techniques of biochemistry, enzymology, molecular and cellular biology, microbiology, genetics and protein and nucleic acid chemistry and hybridization described herein are those well known and commonly used in the art. The methods and techniques of tlie present invention are generally performed according to conventional methods well known in the art and as described in various general and more specific references that are cited and discussed throughout the present specification unless otherwise indicated. See, for example, Sambrook et al. Molecular Cloning: A Laboratory Manual, 2d ed.. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989); Ausubel et aL, Current Protocols in Molecular Biology, Greene Publishing Associates (1992, and Supplements to 2002); Harlow and Lane,

Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1990); Taylor and Drickamer, Introduction to Glycobiology, Oxford Univ. Press (2003); Worthington Enzyme Manual, Worthington Biochemical Corp., Freehold, NJ; Handbook of Biochemistry: Section A Proteins, Vol I, CRC Press (1976); Handbook of Biochemistry: Section A Proteins, Vol II, CRC Press (1976); Essentials of Glycobiology, Cold Spring Harbor Laboratory Press (1999).
The following examples are intended to promote a further understanding of the present invention.
EXAMPLE 1
This example provides method for preparing various Pmt inhibitors. Unless otherwise stipulated all materials were obtained from Sigma-Aldrich Chemical Co, (St. Louis, MO) and used as received. The 1H NMR spectra of all intermediates and final products were in accord with published data.
Preparation of Pmti-1, (5-[t3,4-bis(phenyhnethoxy) phenyl]methyleae]-4-oxo-2-thioxo-3-tliiazolidineacetic Acid), is as follows.

The procedure was adapted from Orchard et al in U.S. Patent No, 7,105,554. A solution of rhodaiiine-3-acetic acid (1 g, 5.20 mmol, 1 eq.), 3,4-diben5!yloxybenzaIdehyde (2.04 g, 6.25 mmol, 1.2 eq.), and sodium acetate (1.3 g, 15,6 mmol, 3 eq.) in acetic acid (30 mL) is heated to reflux, and stirred overnight. As the reaction mixture is cooled to room temperature, the product is precipitated and filtered and washed with acetic acid, then petroleum ether. The residue is dissolved in hot DMSO, filtered, and precipitated by addition of water. Upon cooling, the precipitate is filtered and recrystallized from ethyl acetate and petroleum ether to give a product which is suspended in water and fi-eeze-dried overnight in vacuo to give tlie final product as a fluffy yellow powder.

Preparation of Pmti-2, 2 (5^[[3-(l-Phenylethoxy>4-(2-phenylethoxy)]phenyl]methylenej. 4-oxo-2-thioxo-3-thiazolidineacetic Acid), is as follows.

This product is synthesized according to the directions of Orchard et ah in U.S. Patent No. 7,105,554. A solution of rhodanine-3-acetic acid (375 mg, 1.96 mmol, leq.), 3-(l-phenylethoxy)-4-(2-phenylethoxy)benzaldehyde (680mg, 1.96 mmol,leq.) and ammonium acetate (453 mg, 3eq.) is heated to 70°C for ten minutes,then cooled to room temperature and diluted with ethyl acetate (100 mL). The organic solution is washed with IM HCI (2 x 200 mL) and brine (200 mL) then dried over sodium sulfate and evaporated. The product is purified by liquid chromatography using a 10 x 2.5cm glass column packed with 35-75 |am CIS (Alltech Associates, Deerfield, IL). Gradient elution is employed. Buffer A is 0.1% acetic acid and buffer B is 80% acetonitrile. The gradient is comprised of 20% B for three minutes, increasing to 75% B over 40 minutes. The flow rate is 8 mL/min. Detection is at 280 nm. The appropriate fractions are pooled, concentrated, and freeze-dried in vacuo to give the product as a fluffy yellow powder.
Preparation of Pmti-3, (5-[[3-(l-Phenyl-2-hydroxy)ethoxy)'4-(2" phenylethoxy)]phenyl]methylene]-4-oxo-2-thioxo-3'thiazoIidineacetic Acid), (Orchard etal in U.S. Patent No. 7,105,554) is synthesized m three steps as follows.


Step 1: Production of (+)-(S)-2-Acetoxy-l-bromo-l-phenylethane. Cold HBr-acetic acid (12-4 g, 52-2 mmol) is added dropwise to (-HR>l-phenyIethane-l,2-diol(2-4 g, 17-4 mmol) during about five minutes and the mixture stirred at room temperature for 40 minutes. Water (25 mL) is added and the solution is neutralized with sodium carbonate and extracted with ether (3x30 mL). The combined extracts are dried and evaporated to give (+)-(S)-2-acetoxy-l-bromO'-l-phenylethaue (3-93 g, 93%), ^25 1-415 g/mL, Mo24 + 93-5" (c 5-63 in CCI4) 2'72 (5H, s), 4-98 (aH, dd, 6-7 and 7-0 Hz) and 5-56 (2Ii d).
This product is not distilled. The isomeric homogeneity is established by comparison of the nmr spectrum (absence of PhCH*OAc resonance) with that of 1,2-diacetoxy-l-phenylethane. (Note that racemic reagents are substituted for the optical isomers listed).
Step 2; Production of 3-[(l-Phenyl-'2-hydroxy)ethoxy]-4-(2-phenylethoxy)-'benzaldehyde. (2'Acetoxy-1 -bromoethyl)benzene (3.3 2 g, 13,67 mmol, 1.2 eq) (the product of Step 1), is added to a stirred solution of 3-hydroxy-4-(2-phenylethoxy)-benzaldehyde (2.76 g, 11.39 mmol, 1 eq.) and cesium carbonate (2.97 g, 9J1 mmol, 0.8 eq.) in N,N-dimethylfonnaraide (15 mL). The solution is stirred for 19 hours at room temperature, then 21 hours at 80*^C. The reaction is worked up by partitioning between ethyl acetate and water (brine is added to help break up the emulsion that formed). The organic layer is washed twice more with water, brine, and then dried over sodium sulfate and evaporated to give a dark oil. The residue is purified by chromatography on silica gel and elution with diethyl ether gives an orange oil. This oil is dissolved in methanol (100 ml) and to the solution is added an aqueous solution of sodium hydroxide (7 mL, IM). After 30 minutes, the mixture is evaporated (to remove the methanol) and the residue partitioned between diohloromethane and water. The organic layer is dried over sodium sulfate and evaporated. The residue is purified by chromatographed on silica gel and elution witla petroleum ether:diethyl ether (1:2) gives the product as a cream coloured powder.

step 3: Production of Pmti-3. A solution of rhodanine-3-acetic acid (158 mg, 0.828 mmol, leq.), 3-(l^phenyI'-2-hydroxy)etboxy)-4-(2-phenylethoxy)benzaldehyde (300mg, 0.828 mmol, leq.) (the product of Step 2), and ammonium acetate (191 mg, 3eq) in toluene (10 mL) is heated to reflux for 3.5 hours, cooled to room temperature, and diluted with ethyl acetate (50 mL). The organic solution is washed with IM HCl (2 x 200 mL) and brine (200 mL) then dried over sodium sulfate and evaporated. After work-up, the residue is purified by chromatography on silica gel. Elution with ethyl acetate gives a yellow gum, which is reciystallized from diethyl ether and petroleum ether to give the product as a yellow powder.
EXAMPLE 2
This example shows that Pichia pastoris transformed with an expression vector encoding the Kringle 1-3 marker glycoprotein and treated with Pmt inhibitors produced a glycoprotein having reduced 0-gIycosylation.
Plasmid DNA encoding a His-tagged reporter glycoprotein consisting of human plasminogen domains Kl, K2, and K3 (Kringle 1-3 protein) under the control of the Pichia pastoris alcohol oxidase 1 (AOXl) promoter was transformed into wild-type Vichia pastoris to produce strain yJC53. The Kringle reporter protein consisting of domains Kl, K2, K3, and K4 has been discussed in Duman etal Biotechnol. Appl. Biochem. (1998), v.28, p.39'45 and only domain K3 in Choi et al, 2003, Proc. NatL Acad. Sci. U. S. A, 100(9): 5022-5027. The amino acid sequence of the Kringle 1-3 protein used in the Example is
SECKTGNGKNYRGTMSKTKNGITCQKWSSTSPHRPRFSPATHPSEGIiEENYCRNPDNDPQGPWCyTTDPE KRYDYCDILECEEECMHCSGENYDGKISKTMSGLECQAWDSQSPHAHGYIPSKFPNKNLKKNYCRNPDRE LRPWCFTTDPNKRWELCDIPRCTTPPPSSGPTYQCLKGTGENYRGNVAVTVSGHTCQHWSAQTPHTHSRT PENFPCKNLDENYCRNPDGKRAPWCHTTNSQVRWEYCKIPSCDSSPVSTEQLAPTAPPELTPWQDGGGH HHHHHHHH (SEQ E) NO:l), The Kringle 1-3 protein contains at least two potential mammalian 0-glycosylation sites that conform to the purported consensus sequence P at -1 and +3: the serine residue, which is 0-glycosylated, is capitalized in the amino acid sequence "pppSsgp" and the threonine residue, which is O-glycosylated, is capitalized in the amino acid sequence 'UapTapp". The O-glycosylation sites are underlined in the above amuio acid sequence. The potential mammalian 0-glycosylation sites are located between the Kl and K2 domains and the K2 and K3 domains. However^ as shovra in Table 1, in yeast the protein has about 20 0-linked glycosylation sites. Thus, 0-linked glycosylation can be a significant disadvantage to producing proteins in yeast without inhibiting 0-Iinked glycosylation as shown by the methods herein. An N-glycosylation site resides in the K3 domain, which had been removed by replacing the asparagine at position 208 of SEQ ID N0:1 with a serine. Therefore, the only glycans on the Kringle 1-3 protein would be the result of 0-glycosylation.
Plasmid containing DNA encoding the Kxmgle 1-3 protein was prepared using forward primer K1-3/UP 5'-CGGAA TTCTC AGAGT GCAAG ACTGG GAATA GAA-3' (SEQ ID NO:2) and

reverse primer KI-3/LP1 (Reverse primer, 301^^-21115, paired with K 1-3/UP) 5'-ATGAT GATGA CCACC ACCGT CCTGG ACCAC AGGGG TTAG-3' (SEQ ID N0:3) to produce a PCR product, which was then PCR amplified using reverse primer K1-3/LP2 (Reverse primer, 3GlyH- 9His+stop codon, paired with K 1-3/UP) 5'-TTAAT GATGA TGATG ATGAT GATGA TGATG ACCAC CACC.3' (SEQ ID NO:4), PCR conditions were as follows: after 1 cycle of 95**C for 2 minutes as a denaturation step, the PCR reaction was subjected to 30 cycles of 95°C for 30 seconds, SO^'C for 30 seconds, and 72°C for 1 minute, and then I cycle of 72°C for 10 minutes. After the PCR reaction and column purification of the PCR products, nucleotide A overhangs of the PCR products were generated using ExTaq (1 cycle of 72'C for 15 minutes). The resuhing PCR products were used for the second PCR reaction as a PCR template where the primers, K1-3/UP and K1-3/LP2, were used to amplify wild-type Kringle 1-3 +3Gly+9His, which was cloned into a pCR2,l plasmid vector (Invitrogen) to produce pBKlOS. The following PCR primers were then used to generate an Asn to Ser mutation at position 208 in the Kringle 1-3 protein to produce amino acid sequence NRTP from amino acid sequence SRTP: forward primer K3f (Asn to Ser) 5'-ACCCCTCACACACATTCTAGGACACCAGAAAACTTC^3' (SEQ ID NO:5) and reverse primer K3r 5'-CTGTGCACTCCAGTGCTGACAGGTGTG-3' (SEQ ID NG;6). The Asn to Ser mutation was then generated in pBK105 by the hiverse PCR, PCR conditions were as follows; after 1 cycle of 95'C for 2 minutes as a denaturation step, the PCR reaction was subjected to 35 cycles of 95°C for 30 seconds, 60"C for 30 seconds, and 72°C for 5 minutes, and then 1 cycle of 72*^C for 10 minutes* The resulting PCR products were ligated to produce plasmid pBKl 18, which was sequenced to confirm the mutation.
Plasmid pBKUS was digested with EcoKl and the DNA fragments were gel purified and cloned into the EcoRI sites of pPICZaA (Invitrogen, La Jolla, CA) to produce pBKl 19 {Pichia expression plasmid). Plasmid pPICZaA contains an a-factor secretion signal that allows the efficient secretion of most proteins from Fichia pastoris; 5'-A0X, a 942 bp fragment containing the AOX 1 promoter that allows methanol-inducible and high-level expression in Pichia pastoris; and, the ZEOCIN resistance gene for positive selection in E. coll and Pichia pastoris. Plasmid pBKl 19 was linearized with Pmel before transformuig into Pichia pastoris strains. Plasmid pBK was transformed into Pichia pastoris strain yJC53, a wild-type strain, and various PA/T knockout strains.
/*A/r knockout yeast strains were created in Pichia pastoris following the procedure outlined for Saccharomyces cerevisiae in Gentzsch and Tanner, EMBO J. 1996 Nov l;15(2l): 25752-5759). The Pichia pastoris PKT genes were identified in the nucleotide sequence of the Pichia pastoris genome obtained from Integrated Genomics, Chicago, IL by homology searching using the nucleotide sequences for the Saccharomyces cerevisiae PAfT genes. Deletion of Pichia pastoris PMT(?pPMT) genes was as follows. The PpPMT deletion alleles were generated by the PCR overlap method (See for example, Davidson et al, 2004, Glycobiology 14:399'407; Ho et al, 1989, Gene 77:51-9; Horton et al, 1989, Gene 77:61-8). hi the first PCR reaction, DNA comprising the nucleotide sequences for 5' and 3' flanking regions of the PMP genes and the NAT or HYG resistance markers (Goldstein and McCusker,

1999, Yeast 14:1541-1553; Goldstein ero/., 1999, Yeast 15:507-110) were PCR amplified. The primers sequences for the regions flanking the PMT genes were designed using the Pichia pastor is genome nucleotide sequence obtained from Integrated Genomics, Chicago, IL as a guide. Pichiapastoris genomic DNA was used as a template for the Pp/^J^/T flanking regions PCR amplification, while NAT and HYG fragments were PCR amplified using plasmids as described in (Goldstein, and McCusker, 1999, ibid.\ Goldstein et al, 1999, ibid) as templates. Then, in a second PCR reaction, all three first round PCR products were used as templates to generate an overlap product that contained all three fragments as a single linear allele. The final PCR product was then directly employed for transformation. Transformants were selected on YPD medium containing 200 ^g/mL of hygromycin or 100 p.g/mL of nourseothricin. In each case the proper integration of the mutant allele was confirmed by PCR. The PMT knockout strains created were yJC5l (pmt3d,pmt5A,pmt6A,), yJC55 {pmtlA\ yJC66 {pmt24\ and yJC65 [pmt4A). The PMTknockout strains were each transformed with plasmid pBKl 19 encoding the above Kringle l~3 protein.
Kringle 1-3 protein expression for the transformed yeast strains was carried out at in shake flasks at 24°C with buffered glycerol-complex medium (BMGY) consisting of 1% yeast extract, 2% peptone, 100 mM potassium phosphate buffer pH 6.0, 1.34% yeast nitrogen base, 4 x 10-5 % biotin, and 1% glycerol. The induction medium for protein expression was buffered methanol-complex medium (BMMY) consisting of 1% methanol instead of glycerol in BMGY. Prat inhibitor Pmti-1, Pmti-2, or Pmti-3 in methanol was added to the growth medium to a final concentration of 0.2 ^M, 2 pM, or 20 \]M at the time the mduction medium was added. Cells were harvested and centrifuged at 2,000 rpm for five minutes. The Pmt inhibitors Pmti-l, Pmti"2, and Pmti-3 are essentially interchangeable, with small variations in ease of use. For example, in the cell culture conditions described, the solubility of Pmti-3 is greater than that of Pmti-1 and Pmti-2 and, therefore, the most desirable of the three.
Seven pL of the supernatant from the yJC53 cultures treated with Pmti-1 or yJC55 was separated by polyacrylamide gel electrophoresis (SDS-PAGE) according to Laeramli, U. K. (1970) Nature 227, 680-685 and then electroblotted onto nitroceUulose membranes (Schleicher & Schuell (now Whatman, Inc., Florham Park, NJ). Kringle 10 protein was detected on the Western blots using an anti-His antibody (H-15) from Santa Cruz Biotechnology Inc. (Santa Cruz, CA) and developed using the ImmunoPure Metal Enhanced DAB Substrate Kit (Pierce Biotechnology, Rockford, IL). As shown in Lane 1 of the Western blot shown in Figure 1, Kringle 1-3 protein from untreated Pichia pastoris runs as a smear due to the presence of 0-glycosylation. However, in contrast, Kringle 1-3 protein from yJC53 {Fichlapastoris treated with 2 or 20 nM Pmti-1, lanes 2 and 3, respectively) exhibits a distinct band, due to lack of 0-glycosylation, similar to that of Kringle 1-3 protein expressed from yJC55 {^pmtlA knockout mutant of Pichia pastoris) (lanes 4 and 5). Figure 1 further shows that Pmti-1 reduced O-glycosylation to a level similar to that observed in a strain lacking Pmtl.
To measure 0-glycosylation reduction by the Pmt inhibitors, the Kringle 1-3 protein was purified from the growth medium using nickel chelation chromatography (Choi et a/., 2003, Proc. Natl.

Acad. Sci. U. S. A. 100(9): 5022-5027) and the 0-glycans released from and separated from Kringle 1-3 protein by alkaline elimination (beta -elimination) (Harvey, 1999 Mass Spectrometry Reviews 18, 349-451). This process also reduces the newly formed reducing terminus of the released 0-glycan (either oligomannose or mannose) to mannitol. The mannitol group thus serves as a unique indicator of each O-glycan. 0.5 nmole or more of Kringle 1-3 protein, contained within a volume of 100 ]xL PBS buffer, was required for beta elimination. The sample was treated with 25 pL alkaline borohydride reagent and incubated at 50°C for 16 hours. About 20 pL arabitol internal standard was added, followed by 10 ^ glacial acetic acid. The sample was then centrifuged through a Millipore filter containing both SEPABEADS and AG 50W-X8 resin and washed with water. The samples, including wash, were transferred to plastic autosampler vials and evaporated to dryness in a centrifugal evaporator. 150 \i\ 1% AcOH/MeOH was added to the samples and the samples evaporated to dryness in a centrifugal evaporator. This last step was repeated five more times. 200 pL of water was added and 100 pL of the sample was analyzed by high pH anion-exchange chromatography coupled with pulsed electrochemical detection-Dionex HPLC (HPAEC-PAD). Average 0-glycan occupancy was determined based upon the amount of mannitol recovered. The results are summarized in Table 1, which shows that any one of the Pmt chemical inhibitors reduced 0-linked glycosylation of secreted Kringle 1-3 protein in the Pichia pastoris strains that contained intact PMTl and PMT2 genes to a level that was comparable to the level of 0-linked glycosylation seen for cells containing deletions of either the PMTl or PMT2 gene. Table 1 also shows that while the protein has two potential mammalian 0-linked glycosylation sites, in yeast the protein has about 20 0-linked glycosylation sites.

Table 1
Strain Relevant Genotype Treatment O-Glycan Occupancy!
yJC53 Wild-Type 0 20


2 )3M Pmti-l 9
yJC51 pmtA3^5,A6 0 17


2 ^iM Pmti-l 6


20 \M Pmti-l 4


0.2^Pmti-2 3


2 nM Pmti-2 2


0,2 yM Pmti-3 6


2 \M Pmti-3 4
yJC55 pmtAl 0 3


2 ^M Pmti-l 2


20 [iM Pmti-l 2
yJC66 pmtA2 0 4


2 [iM Pmti-l 4


20^iMPmti'l 4
yJC65 pmtA4 0 18


2 ^lM Pmti-l 7


20 jiM Pmti-l 4
1 average number of 0-linked mannose chains per protein.
EXAMPLE 3
In this example, yeast cells transformed with DNA encoding the T. reesei a-mannosidase results in production of proteins with reduced 0-glycosylation and that the O-glycosylation was further reduced when the cells were also incubated in the presence of a Pmt inhibitor.
The H "f- L, chains of an anti~Her2 monoclonal antibody were expressed in Pichio pastor is strains GS115 (WT) and GSllS that was genetically engineered to co-expressed T. reesei a-mannosidase (+Trman). GSl 15 is available from Invitrogen (Carlsbad, CA) and, with the exception of a HIS4 mutation to enable his4 selection, has an essentially wild type phenotype. The H + L chains were expressed as two separate genes from plasmid pJC284, which was derived from Invitrogen plasmid pA0815.
The H + L genes were generated using anti-Her2 antibody sequences obtained from GenBank. The GenBank accession number for the L chain is 1N8Z_A and the GenBank accession number for the H chain variable region plus CHI domain is 1N8Z_B. Tlie GenBank accession number

for the H chain Fc region is BC092518. Both the H and L chain DNA sequences were codon optimized according to Pichia pastoris codon usage to enhance translation in Pichiapastoris, Optunization of codons for use in Pichia sp. is well known in the art and has been described in, for example, Outchkourov etal, 2002,Protein Expr. Purif 24:18-24; Sharp and Li, 1987, Nucleic Acids Res. 15:1281-95; Woo JH, Liu et al., 2002, Protein Expression and Purification 25:270-282, and, Nakamura, et al, 2000, Nucleic Acids Res. 28:292. Constant regions of the H chain (human IgGl) and L chain (human Kappa) were synthesized by GeneArt Inc., Regensburg, Germany. Variable regions were made in-house using oligonucleotides purchased from IDT toe. (Coralville, lA) in an overlapping PCR method. Full length H and L chains were assembled by overlappmg PCR, and resulting H and L chains were cloned into pCR2.1 TOPO vector (tovitrogen. La Jolla, CA) to generate pDX344 and pDX349, respectively. H + L chains from pDX344 and pDX349 were combined with GAPDH promoters and AOXl tenninator sequences in vector pDX580 (backbone derived Srom Invitrogen vector pGAPZA). Finally, the H + L chain expression cassettes were subcloned from pDX580 into vector pJC284. The nucleotide sequence of the codon-optimized DNA encoding the light chain is shown in SEQ ID N0:7 and the nucleotide sequence of the codon-optimized DNA encoding the heavy chain is shown in SEQ ID NO; 8. Plasmid pJC284 has GAPDH promoters for expressmg the H + L genes and an intact his4 gene for selection of transformants in strain GSI15 and GS115(+Trman). Yeast strains GS115 and GS115(+Trman) were transformed with pJC285 and transformants with the plasmid integrated into the genome at the his4 locus were isolated to produce strains that produced the anti-Her2 antibody.
Construction of strain GS115(+Trman) was as follows. The Trichoderma reesei a-1,2-mannosidase was expressed from an expression cassette in plasmid pJC285. Plasmid pJC285 was derived from Invitrogen vector pGAPZA, which has the Zeocin resistant gene as the selectable marker, and contains an expression cassette comprising DNA encoding the T. reesei a-l,2~mannosidase catalytic domain (SEQ ID N0:9) with the first 84 base pairs encoding its signal sequence replaced with a DNA encoding the Saccharomyces cerevistea aMAT pre signal sequence (SEQ ID NO:10), which encodes just the ER-targeting amino acids, operably linked to DNA comprising the Pichiapastoris GAPH promoter (SEQ ID NO: 11) at the 5' end and DNA comprising the Pichiapastoris AOXl transcription termination sequence (SEQ ID NO: 12) at the 3' end. The nucleotide sequence of the complete expression cassette is set forth in SEQ ID N0:13. Yeast strain GSl 15 was transformed with pJC285 and transformants with the plasmid mtegrated into the genome at the GAPDH locus were isolated to produce strain GS115(+Tnnan).
Duplicate cultures of the strains were cultured in 200 mL of buffered dextrose-complex medium (BMDY) consisting of 1% yeast extract, 2% peptone, lOOmM potassium phosphate pH 6.0, 1.34% Yeast Nitrogen Base, .00004% biotin, 2% dextrose, and with or without Pmti'.2 at 0.3 or 0.03 MM. Following 72 hours of growth, culture supematants were collected and centrifuged to remove yeast cells. Antibody in the remainmg supernatant fraction (about 200 mL) was purified over a Protein A column and subjected to O-glycan analysis as described in Example 2. to addition to the mannitol assay, the average

length of O'linked mannose chains was determined by chromatographic analysis without hydrolysis. The results are summarized in Table 2,
There are 14 yeast 0-glycan sites on the antibody. When the antibody was produced in the wiid-type GSl 15 strain, all 14 0-glycan sites have glycan structures with 8% of the sites having just one mannose, 39% having a two mannose chain, 43% having a three mannose chain, and 9% having a four mannose cham (See Table 2). However, when the antibody was produced in wild-type cells treated with the chemical inhibitor Pmti-2, only two of the 14 0-glycan sites were occupied and for 76% of the two sites, the mannose chain had only one mannose residue. Neither of the two sites had a mannose chain with three or four mannose residues. It should be noted that the analysis did not determine which two of the 14 sites were occupied. Either any combination of two 0-glycan sites per antibody molecule were occupied or particular-0-gIycan sites are preferentially occupied. In the latter case, to provide antibodies (or other proteins) completely devoid of 0-glycans, the amino acid sequences comprising the preferred 0-gIycan sites can be modified to amino acid sequences that eliminates 0-Unked glycosylation at the sites.
Table 2 fiirther shows that when the antibody was produced in cells that included DNA encodmg the Tricoderma reesei a-l,2-maanosidase (strain GSl 15(-i-Trman)), only four of the 14 O-glycan sites were occupied and for 95% of the four sites, the mannose chain had only one mannose residue. None of the four sites had a mannose chain with three or four mannose residues. If particular sites are preferentially O-glycosylatedi to provide antibodies (or other proteins) completely devoid of O-glycans, the ammo acid sequences comprising the preferred O-glycan sites can be modified to amino acid sequences that eliminates O-glycosylation at the sites.
Finally, Table 2 shows that when the antibody was produced in cells that included DNA encoding the Tricoderma reesei a-l,2-mannosidase and in the presence of Pmti-2, only one of the 14 O-glycan sites was occupied and for 91% of the sites, the mannose chain had only one mannose residue. No mannose chain had three or four mannose residues. If only one or only a few sites are preferentially O-glycosylated, to provide antibodies (or other proteins) completely devoid of O-glycans, the amino acid sequences comprising the preferred 0-glycan sites can be modified to amino acid sequences that eliminate O-glycosylation at the sites
Table 2 turther shows that 0.3 uM of Pmti inhibitor is sufficient to reduced the occupancy by about 86% and the chain length for 76% of the molecules to one mannose while allowing the culture to grow. Including the Tricoderma reesei a-l,2-mannosidase allowed the amount of Pmti inhibitor to be reduced by 10 fold and the occupancy reduced to 93% and the chain length for 87% of the molecules to one mannose. These results show that using an amount of Pmti inhibitor that does not kill the cells is sufficient to produce glycoproteins having reduced 0~linked glycosylation. These results further show that the Pmti inhibitor and the a-mannosidase appear to synergistically reduce the amount of 0-linked glycosylation.


Seven jiL of the supernatant for each of the above were reduced and subjected to SDS-PAGE and Western blotting using an HRP-conjugated anti-human IgG (H&L) to detect H and L chains. The results are shown in Figure 2, The hyper 0-glycosylated H chain is the slowest migrathig band visible in the first pair of lanes in Figure 2. Figure 2 shows that there is a decrease in the amount of O-glycosylated heavy chain when the antibody was coexpressed with Tricoderma reesei a-l,2-mannosidase or the cells expressing the antibody was incubated in the presence of the Pmti-2 inhibitor, or when the antibody was coexpressed with Tricoderma reesei a'-l,2-mannosidase and the cells expressing both proteins was incubated in the presence of the Pmti-2 inhibitor.
While the present invention is described herein with reference to illustrated embodiments J it should be understood that the invention is not limited hereto. Those having ordmary skill in the art and access to the teachings herein will recognize additional modifications and embodiments within the scope thereof. Thereforcj the present invention is limited only by the claims attached herein.











WHAT IS CLAIMED:
1. A method of producing a proteiB having reduced O-linked glycosylation
comprising:
(a) providing a nucleic acid encoding a protein;
(b) introducing tihe nucleic acid into a host cell to provide a culture of the host cell;
(c) contacting the culture with one or more inhibitors of Pmt-mediated 0-linked glycosylation; and
(d) isolating the glycoprotem produced by the host cell in the presence of the one or more inhibitors to produce the protein having reduced O-linked glycosylation.

2. The method of Claim 1 wherein the cultui"e is grown for a time sufficient to provide a multiplicity of the host cells having the nucleic acid before contacting the culture with the one or more inhibitors of Pmt-mediated 0-linked glycosylation.
3. The method of Claim 1 wherein the culture is grown in the presence of the one or more inhibitors of Pmt-mediated 0-linked glycosylation.
4. The method of Claim 1 wherein the nucleic acid is operably linked to an inducible promoter.
5. The method of Claim 4 wherein the culture is grown for a time sufficient to provide a multiplicity of the host cells having the nucleic acid before contacting the culture with the one or more inhibitors of Pmt-mediated 0-linked glycosylation and an inducer of the promoter to induce expression of the protein and isolating the protein produced by the host cell in the presence of the one or more inhibitors and the mducer to produce the protein having reduced 0-linked glycosylation.
6. The method of Claim 4 wherein the culture is contacted with an inducer of the promoter to induce expression of the protein for a time before contacting the culture with the one or more inhibitors of Pmt-mediated 0-linked glycosylation and isolating the protein produced by the host cell in the presence of the one or more inhibitors and the inducer to produce the protein having reduced O-linked glycosylation.
7. The metliod of claim 1 wherein the one or more inhibitors is a benzylidene thiazoUdinedione.

8. The method of claim 7 wherein the one or more inhibitors are selected from the group consisting of: 5-[[3,4-bis(phenybnethoxy) phenyl]methylene]-4"OXo-2-1iiioxo-3-thia2olidineacetic Acid; 5-[[3-(l-Phenylethoxy>4-(2'phenylethoxy)]phenyl]methylene]-4-oxo-2-thJoxo-3-thia2oIidineacetic Acid; and 54[3-(l-Phenyl"2-hydroxy)ethoxy)-4-(2-phenylethoxy)]phenyl]methylene]-4"OXO"2^thioxo-3-thiazolidineacetic Acid.
9. The method of claim 1, wherein the host cell is a fungal cell.
10. The method of claim 1, wherein the host cell is a yeast cell.
1L The method of claim 1, wherein the host ceil is selected from the group consisting ofK, lactis^ Fichiapastoris, Pichia methcmolica^ and Hamenula,
12. The method of claim 1, wherein the host cell is Pichia pastoris.
13. The method of claim 1, wherein the host cell is a yeast or filajuentous iungal cell
that has been genetically modified to produce glycoproteins with a predommant N-glycan glycoform.
14» The method of claim 1 wherein the host cells have been genetically modified to produce glycoproteins m which the N-glycosylation pattern is human-like or humanized*
15. The method of claim 1 wherein the glycoprotein is produced at a yield of at least 100 mg/Uter of culture medium.
16. A method of producing a protein having reduced O-linked glycosylation comprising:

(a) providing a nucleic acid encoding a protein;
(b) introducing the nucleic acid into a host cell to provide a culture of the host cell;
(c) contacting the culture with one or more a-l,2'mannosidase en2ymes; and
(d) isolating the protem produced by the host cell in the presence of die one or more a-l,2-mannosidase enzymes to produce the glycoprotein having reduced O-linked glycosylation,
17. The method of Claim 16 wherein the culture is grown for a time sufficient to
provide a multiplicity of the host cells having the nucleic acid before contacting the culture with the one
or more a-l,2"mannosidase enzymes.

18. The method of Claim 16 wherein the culture is grown in the presence of the one or more a-l,2-mannosidase enzymes.
19. The method of Claim 16 wherein a second nucleic acid encoding the one or more a-l,2'mannosidase enzymes is provided and introducing the second nucleic acid into the host cell.
20. The method of Claim 16 wherein a second nucleic acid encoding the one or more a*-l,2-mannosidase enzymes operably linked to an inducible promoter is provided and introducing the second nucleic acid into the host cell.
21. The method of Claim 20 wherein the culture is grown for a tune sufficient to provide a multiplicity of the host cells before inducing expression of the protein and the one or more a-l,2-mannosida3e enzymes to produce the protein having reduced 0-linked glycosylation.
22. The method of Claim 20 wherein expression of the protein is induced for a time before inducing expression of the one or more a-l,2-mannosidase enzymes to produce the protein having reduced O-linked glycosylation.
23. The method of Claim 20 wherein expression of the one or more a-1,2" mannosidase enzymes is induced for a tune before inducing expression of the protein to produce the protein having reduced O-linked glycosylation.
24. The method of claim 16 wherem the a-l,2-mannosidase is from Trichoderma reesei^ Saccharomyces sp., or Aspergillus sp.
25. The method of claim 16 wherein the a-l,2-mannosidase is from Trichoderma reesei.
26. The method of claim 16, wherein the host cell is a fungal cell.
27. The method of claim 16, wherein the host cell is a yeast cell.
28. The method of claim 16, wherein the host cell is selected from the group consisting ofK. lactis, Pichiapastoris, Pichia methanolica, and Hansenula.
29. The metliod of claim \6^ wherein the host cell is Pichiapastoris.

30. The method of claim 16, wherein the host cell is a yeast or filamentous fungal cell that has been genetically modified to produce glycoproteins with a predominant N-glycan glycoform.
31. The method of claim 16 wherein the host cells have been genetically modified to produce glycoproteins in which the N-glycosylation pattern is human-like or humanized.
32. The method of claim 16 wherein the glycoprotein is produced at a yield of at least 100 mg/liter of culture medium.
33. A method of producing a protein having reduced 0-linked glycosylation comprising:

(a) providing a nucleic acid encoding a protein operably linked to an inducible promoter;
(b) introducing the nucleic acid into a host cell and growing the host cell containing the nucleic acid to produce a culture of the host cell;
(c) contactmg the culture with one or more inhibitors of Pmt-mediated 0-luiked glycosylation and one or more one or more a-ij2-mannosidase enzymes; and
(d) isolating the glycoprotein produced by the host cell in the presence of the one or more inhibitors and the one or more one or more a-l,2-mannosidase enzymes to produce the protein having reduced 0-lmked glycosylation.

34. The method of Claim 33 wherein the culture is grown for a time sufficient to provide a multiplicity of the host cells having the nucleic acid before contacting the culture with the one or more inhibitors of Pmt-mediated 0-luiked glycosylation.
35. The method of Claim 33 wherein the culture is grown in the presence of the one or more inhibitors of Pmt-raediated 0-linked glycosylation.
36. The method of Claim 33 wherein the nucleic acid is operably linked to an inducible promoter.
37. The method of Claim 36 wherein the culture is grown for a time sufficient to provide a multiplicity of the host cells having the nucleic acid before contacting the culture with the one or more inhibitors of Pmt-mediated 0-linked glycosylation and an inducer of the promoter to induce expression of the protein and isolating the protein produced by the host cell in the presence of the one or more inhibitors and die inducer to produce the protein having reduced O-linked glycosylation.

38. The method of Claim 36 wherein the culture is contacted with an inducer of the promoter to induce expression of the protein for a time before contactmg the culture with the one or more inhibitors of Pmt-mediated O-linked glycosylation and isolating the protein produced by the host cell in the presence of the one or more inhibitors and the inducer to produce the protein having reduced O-linked glycosylation.
39. The method of Claim 33 whereui the culture is grown for a time sufficient to provide a muhiplicity of the host cells having the nucleic acid before contacting the culture with the one or more a-l,2-mannosidase enzymes.
40. The method of Claim 33 wherein the culture is grown in the presence of the one or more a-l,2-maimosidase enzymes.
41. The method of Claim 33 wherein a second nucleic acid encoding the one or more a-1,2-mannosidase enzymes is provided and introducing the second nucleic acid into the host cell.
42. The method of Claim 33 wherein a second nucleic acid encoding the one or more a-l,2-mannosidase enzymes operably linked to an inducible promoter is provided and introducing the second nucleic acid into the host cell.
43. The method of Claim 42 wherem the culture is grown for a time sufficient to provide a multiplicity of the host cells before inducing expression of the protem and the one or more a-1,2-mannosidase enzymes to produce the protein having reduced 0-linked glycosylation.
44. The method of Claim 42 wherein expression of the protein is mduced for a time before inducing expression of the one or more a-l,2-mannosidase enzymes to produce the protein having reduced O-luiked glycosylation.
45. The method of Claim 42 wherein expression of the one or more a-1,2-mannosidase enzymes capable of inhibiting 0-linked glycosylation is induced for a tune before inducing expression of the protein to produce the protein having reduced 0-linked glycosylation,
46. The method of claim 33 wherem the one or more inhibitors is a benzylidene thiazolidinedione.
47. The method of claim 33 wherein the one or more inhibitors are selected from the group consisting of: 5-[[3,4-bis(phenylmethoxy) phenyl]methylene]-4-oxo-2-thioxo-3-thiazolidineacetic

Acid; 5~[[3 -(1 -Phenylethoxy)-4-(2-phenylethoxy)]phenyl]methylene]-4-oxo-2-thioxo-3 -thiazolidineacetic Acid; and 5-[[3-(l-Phenyl-2-hydroxy)ethoxy)-4-(2-phenylethoxy)]phenyl3meliiylene]-4-oxO"2-thioxo-3-thiazolidineacetic Acid.
48. The method of claim 33 wherein the a-l,2-mannosidase is from Trichoderma reesei, Saccharomyces sp., or Aspergillus sp.
49. The method of claim 33 wherein the a-l,2-maimosidase is from Trichoderma reesei,
50. The method of claim 33 wherein the host cell includes a second nucleic acid, which encodes the a-l,2-mannosidase, operably linked to an mducible promoter.
51. The method of claim 33, wherein the host cell is a fungal cell
52. The method of claim 33, wherein the host cell is a yeast cell.
53. The method of claim 33, wherein the host cell is selected from the group consisting ofK, lactis, Pichiapastoris, Pichia methanolica, and Hansenula.
54. The method of claim 33, wherein the host coll is Pichia pastoris.
55. The method of claim 335Vherein the host cell is ayeast or filamentous fungal cell that has been genetically modified to produce glycoproteins with a predominant N-glycan glycoform.
56. The method of claim 33 wherein the host cells have been genetically modified to produce glycoproteins m which the N-glycosylation pattern is human-like or humanized.
57. The method of claim 33 wherein the glycoprotein is produced at a yield of at least 100 mg/liter of culture medium.
58. A glycoprotein composition comprising a predominant species of N-glycan structure and having reduced 0-linked glycosylation.
59. The glycoprotein of Claim 58 wherein the N-giycan structure is selected from the group consisting of Man5GlcNAc2, Man3GlcNAc2, GlcNAcMan5GlcNAc2, GlcNAcMan3GlcNAc2, GlcNAc2Man3GlcNAc2, GalGlcNAcMan5GlcNAc2, Gal(GlcNAc)2

M«nsG)cNAc2, (GaIGlcNAc)2Maa5GlcNAc2. NANAOaiaicNAcMan3GlcNAc2> NANA2Gal2GIcNAcMan3GlcNAc2, and GalGlcNAcMan3GlcNAc?.

Documents:

2110-CHENP-2008 AMENDED CLAIMS 11-09-2013.pdf

2110-CHENP-2008 CORRESPONDENCE OTHERS 31-10-2014.pdf

2110-CHENP-2008 CORRESPONDENCE OTHERS 25-09-2013.pdf

2110-CHENP-2008 CORRESPONDENCE OTHERS 03-12-2014.pdf

2110-CHENP-2008 CORRESPONDENCE OTHERS 11-09-2013.pdf

2110-CHENP-2008 FORM-1 11-09-2013.pdf

2110-CHENP-2008 FORM-13 11-09-2013.pdf

2110-CHENP-2008 FORM-3 11-09-2013.pdf

2110-CHENP-2008 OTHER PATENTDOCUMENT 11-09-2013.pdf

2110-CHENP-2008 AMENDED CLAIMS 17-12-2014.pdf

2110-CHENP-2008 AMENDED PAGES OF SPECIFICATION 17-12-2014.pdf

2110-CHENP-2008 EXAMINATION REPORT REPLY RECEIVED 17-12-2014.pdf

2110-CHENP-2008 OTHERS 11-09-2013.pdf

2110-CHENP-2008 POWER OF ATTORNEY 12-10-2009.pdf

2110-CHENP-2008 FORM-18 12-10-2009.pdf

2110-chenp-2008-abstract.pdf

2110-chenp-2008-claims.pdf

2110-chenp-2008-correspondnece-others.pdf

2110-chenp-2008-description(complete).pdf

2110-chenp-2008-drawings.pdf

2110-chenp-2008-form 1.pdf

2110-chenp-2008-form 3.pdf

2110-chenp-2008-form 5.pdf

2110-chenp-2008-other documents.pdf

2110-chenp-2008-pct.pdf

abs-2110-chenp-2008.jpg

Petition under 137_Regularizing irregularity_IPA3832.pdf

Petition Under Rulr 137.pdf


Patent Number 264478
Indian Patent Application Number 2110/CHENP/2008
PG Journal Number 01/2015
Publication Date 02-Jan-2015
Grant Date 31-Dec-2014
Date of Filing 28-Apr-2008
Name of Patentee GLYCOFI, INC
Applicant Address SUITE 200, 21 LAFAYETTE STREET LEBANON, NH 03766
Inventors:
# Inventor's Name Inventor's Address
1 BOBROWICZ, PIOTR SUITE 200, 21 LAFAYETTE STREET LEBANON, NH 03766
2 COOK, JAMES, W SUITE 200, 21 LAFAYETTE STREET LEBANON, NH 03766
3 KETT, WARREN SUITE 200, 21 LAFAYETTE STREET LEBANON, NH 03766
PCT International Classification Number C12P 21/06
PCT International Application Number PCT/US2006/043535
PCT International Filing date 2006-11-10
PCT Conventions:
# PCT Application Number Date of Convention Priority Country
1 60/737,108 2005-11-22 U.S.A.