Title of Invention	"A KIT FOR DETECTION OF DETERGENT IN MILK"
Abstract	A kit for detection of detergent in milk, particularly in synthetic milk comprising of a solution of Methylene blue dye, the said Methylene blue dye solution is prepared by mixing 5 to 20mg of methylene blue dye in 100ml of distilled water, solution of Ferric Chloride the said Ferric Chloride solution is prepared by mixing 0.3244 to 3.244gm of ferric chloride (anyhydrous) in 100ml of distilled water, a solution of Ethylene diamine tetra acetic acid disodium salt and chloroform, the said Ethylenediamine tetra acetic acid disodium salt solution is prepared by mixing 0.9306 to 9.306gm of ethylenediamine tetra acetic disodium salt (containing 2 molecules of water) in 100ml distilled water. A kit for detection in milk substantially as herein described with reference to, and as illustrated by, the examples.

Title of Invention

"A KIT FOR DETECTION OF DETERGENT IN MILK"

Abstract

A kit for detection of detergent in milk, particularly in synthetic milk comprising of a solution of Methylene blue dye, the said Methylene blue dye solution is prepared by mixing 5 to 20mg of methylene blue dye in 100ml of distilled water, solution of Ferric Chloride the said Ferric Chloride solution is prepared by mixing 0.3244 to 3.244gm of ferric chloride (anyhydrous) in 100ml of distilled water, a solution of Ethylene diamine tetra acetic acid disodium salt and chloroform, the said Ethylenediamine tetra acetic acid disodium salt solution is prepared by mixing 0.9306 to 9.306gm of ethylenediamine tetra acetic disodium salt (containing 2 molecules of water) in 100ml distilled water. A kit for detection in milk substantially as herein described with reference to, and as illustrated by, the examples.

Full Text	FIELD OF INVENTION This invention relates to a kit for detection of detergent in milk. Detergent is one of the ingredients of 'synthetic milk' and therefore the kit can be used for detection of adulteration of milk with 'synthetic milk'. PRIOR ART The limits have been set for the level of detergent in sewage plant, rivers and streams for avoiding frothing. Detergent is also used in industries for cleaning of pipelines/containers and if these pipelines/containers are not washed properly with plentiful water, the detergent may appear in food processed through them. Often, reports appear that detergent is one of the ingredients used in preparation of 'synthetic milk' which is being used for adulterating milk. Therefore, a need has been felt to develop test for detection of detergent in milk which could become the basis for detection of 'synthetic milk' adulteration in milk. A qualitative method applicable for detection of detergent in milk is available and this method uses methylene blue dye solution, ethanol and chloroform. This method works well if the level of 'synthetic milk' in milk is more than 10%. Between 5-10% 'synthetic milk' in milk, the results are ambiguous. The existing method can not detect presence of 'synthetic milk' in milk if the level of 'synthetic milk' is less than 5%. The existing method takes at least 20 min. OBJECTS OF THE INVENTION The object of this invention is to provide a kit for detection of adulteration of 'synthetic milk' in milk up to 1.25%. Another object of this invention is to provide a kit for detection of detergent in milk which can detect up to 12.5 mg of detergent in 100 ml milk. Yet another object of this invention is to provide a kit for detection of detergent in milk wherein the results are provided in 5 to 10 minutes. STATEMENT OF INVENTION According to this invention there is provided a kit for detection of detergent in milk comprising of a solution of Methylene blue dye, a solution of Ferric chloride, a solution of Ethylenediamine tetra acetic acid disodium salt and Chloroform. DETAILED DESCRIPTION The kit for detection of detergent in milk consist of (i) Methylene blue dye solution wherein the solution is prepared by dissolving 5 to 20 mg of methylene blue dye in 100 ml of distilled water (ii) Ferric Chloride solution (20-200 mM) wherein 0.3244 gm to 3.244 gm of ferric chloride (anhydrous) is dissolved in 100 ml of distilled water (iii) Ethylenediamine tetra acetic acid disodium salt solution (25-250 mM) wherein 0.9306 gm to 9.306 gm of ethylenediamine tetra acetic acid disodium salt (containing 2 molecules of water) is dissolved in 100 ml distilled water and (iv) a non polar solvent such as Chloroform. METHOD OF USE METHOD 1- This method uses only two of the solutions/solvent from kit. One milliliter of milk is mixed with 1 ml of dye solution and then 2 ml of chloroform is added to a test tube (15 ml). The contents were then vortexed for about 15 seconds and centrifuged at about 1100 rpm for 3 min. Intensities of blue colour in lower and upper layers are noted. Relatively, more intense blue colour in lower layer indicates presence of detergent in milk. Relatively more intense blue colour in upper layer indicates absence of detergent in milk. Method 2- This method uses all the four solutions/solvent from the kit. One milliliter of milk, 1 ml methylene blue, 50 microlitre of ferric chloride, 3 ml chloroform and 1 ml ethylenediamine tetra acetic acid disodium salt solution are added to test tube (15 ml). The contents are vortexed for 15 seconds and then centrifuged (1100 rpm, 3 min}. Relatively more intense blue colour in lower layer indicates presence of detergent in milk. Relatively more intense blue colour in upper layer indicates absence of detergent in milk. Milk contains fat and it has been observed that defatted milk in Method 1 and Method 2 provides better clarity in results. Defatted milk can be prepared by centrifuging milk at 15°C for 10 minute, followed by removal of fat accumulated at top surface of milk present in centrifuge tube. It is to be noted that the formulation of the present invention is susceptible to modifications, adaptations and changes by those skilled in the art. Such variant formulations are intended to be within the scope of the present invention which is further set forth under the following claims:- WE CLAIM: 1. A kit for detection of detergent in milk, particularly in synthetic milk comprising of a solution of Methylene blue dye, the said Methylene blue dye solution is prepared by mixing 5 to 20mg of methylene blue dye in 100ml of distilled water, solution of Ferric Chloride the said Ferric Chloride solution is prepared by mixing 0.3244 to 3.244gm of ferric chloride (anyhydrous) in 100ml of distilled water, a solution of Ethylene diamine tetra acetic acid disodium salt and chloroform, the said Ethylenediamine tetra acetic acid disodium salt solution is prepared by mixing 0.9306 to 9.306gm of ethylenediamine tetra acetic disodium salt (containing 2 molecules of water) in 100ml distilled water. 2. A kit for detection in milk substantially as herein described with reference to, and as illustrated by, the examples.

Full Text

FIELD OF INVENTION
This invention relates to a kit for detection of detergent in milk. Detergent is one of the ingredients of 'synthetic milk' and therefore the kit can be used for detection of adulteration of milk with 'synthetic milk'.
PRIOR ART
The limits have been set for the level of detergent in sewage plant, rivers and streams for avoiding frothing. Detergent is also used in industries for cleaning of pipelines/containers and if these pipelines/containers are not washed properly with plentiful water, the detergent may appear in food processed through them. Often, reports appear that detergent is one of the ingredients used in preparation of 'synthetic milk' which is being used for adulterating milk. Therefore, a need has been felt to develop test for detection of detergent in milk which could become the basis for detection of 'synthetic milk' adulteration in milk. A qualitative method applicable for detection of detergent in milk is available and this method uses methylene blue dye solution, ethanol and chloroform. This method works well if the level of 'synthetic milk' in milk is more than 10%. Between 5-10% 'synthetic milk' in milk, the results are ambiguous. The existing method can not detect presence of 'synthetic milk' in milk if the level of 'synthetic milk' is less than 5%. The existing method takes at least 20 min.
OBJECTS OF THE INVENTION
The object of this invention is to provide a kit for detection of adulteration of 'synthetic milk' in milk up to 1.25%.
Another object of this invention is to provide a kit for detection of detergent in milk which can detect up to 12.5 mg of detergent in 100 ml milk.
Yet another object of this invention is to provide a kit for detection of detergent in milk wherein the results are provided in 5 to 10 minutes.
STATEMENT OF INVENTION
According to this invention there is provided a kit for detection of detergent in milk comprising of a solution of Methylene blue dye, a solution of Ferric chloride, a solution of Ethylenediamine tetra acetic acid disodium salt and Chloroform.
DETAILED DESCRIPTION
The kit for detection of detergent in milk consist of (i) Methylene blue dye solution wherein the solution is prepared by dissolving 5 to 20 mg of methylene blue dye in 100 ml of distilled water (ii) Ferric Chloride solution (20-200 mM) wherein 0.3244 gm to 3.244 gm of ferric chloride (anhydrous) is dissolved in 100 ml of distilled water (iii) Ethylenediamine tetra acetic acid disodium salt solution (25-250 mM) wherein 0.9306 gm to 9.306 gm of ethylenediamine tetra acetic acid disodium salt (containing 2 molecules of water) is dissolved in 100 ml distilled water and (iv) a non polar solvent such as Chloroform.
METHOD OF USE
METHOD 1- This method uses only two of the solutions/solvent from kit. One milliliter of milk is mixed with 1 ml of dye solution and then 2 ml of chloroform is added to a test tube (15 ml). The contents were then vortexed for about 15 seconds and centrifuged at about 1100 rpm for 3 min. Intensities of blue colour in lower and upper layers are noted. Relatively, more intense blue colour in lower layer indicates presence of detergent in milk. Relatively more intense blue colour in upper layer indicates absence of detergent in milk.
Method 2- This method uses all the four solutions/solvent from the kit. One milliliter of milk, 1 ml methylene blue, 50 microlitre of ferric chloride, 3 ml chloroform and 1 ml ethylenediamine tetra acetic acid disodium salt solution are added to test tube (15 ml). The contents are vortexed for 15 seconds and then centrifuged (1100 rpm, 3 min}. Relatively more intense blue colour in lower layer indicates presence of detergent in milk. Relatively more intense blue colour in upper layer indicates absence of detergent in milk.
Milk contains fat and it has been observed that defatted milk in Method 1 and Method 2 provides better clarity in results. Defatted milk can be prepared by centrifuging milk at 15°C for 10 minute, followed by removal of fat accumulated at top surface of milk present in centrifuge tube.
It is to be noted that the formulation of the present invention is susceptible to modifications, adaptations and changes by those skilled in the art. Such variant formulations are intended to be within the scope of the present invention which is further set forth under the following claims:-

WE CLAIM:
1. A kit for detection of detergent in milk, particularly in synthetic milk
comprising of a solution of Methylene blue dye, the said Methylene blue
dye solution is prepared by mixing 5 to 20mg of methylene blue dye in
100ml of distilled water, solution of Ferric Chloride the said Ferric
Chloride solution is prepared by mixing 0.3244 to 3.244gm of ferric
chloride (anyhydrous) in 100ml of distilled water, a solution of Ethylene
diamine tetra acetic acid disodium salt and chloroform, the said
Ethylenediamine tetra acetic acid disodium salt solution is prepared by
mixing 0.9306 to 9.306gm of ethylenediamine tetra acetic disodium salt
(containing 2 molecules of water) in 100ml distilled water.
2. A kit for detection in milk substantially as herein described with reference to, and as illustrated by, the examples.

Documents:

1970-DEL-2006-Abstract-(13-01-2012).pdf

1970-del-2006-Abstract-(25-08-2014).pdf

1970-del-2006-abstract.pdf

1970-del-2006-assignment (14-01-2008).pdf

1970-DEL-2006-Claims-(13-01-2012).pdf

1970-del-2006-Claims-(25-08-2014).pdf

1970-del-2006-claims.pdf

1970-DEL-2006-Correspondence Others-(13-01-2012).pdf

1970-del-2006-Correspondence Others-(25-08-2014).pdf

1970-del-2006-correspondence-others (14-01-2008).pdf

1970-del-2006-correspondence-others (20-02-2008).pdf

1970-del-2006-correspondence-others-1.pdf

1970-del-2006-correspondence-others.pdf

1970-del-2006-description (complete).pdf

1970-DEL-2006-Form-1-(13-01-2012).pdf

1970-del-2006-form-1.pdf

1970-del-2006-form-18.pdf

1970-del-2006-form-2.pdf

1970-del-2006-gpa (20-02-2008).pdf

1970-del-2006-gpa.pdf

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Patent Number

264661

Indian Patent Application Number

1970/DEL/2006

PG Journal Number

03/2015

Publication Date

16-Jan-2015

Grant Date

14-Jan-2015

Date of Filing

04-Sep-2006

Name of Patentee

NATIONAL RESEARCH DEVELOPMENT CORPORATION,

Applicant Address

"ANUSANDHAN VIKAS", 20-22, ZAMROODPUR COMMUNITY CENTRE, KAILASH COLONY EXTENSION, NEW DELHI-110 048.

Inventors:

#	Inventor's Name	Inventor's Address
1	DR. Y.S. RAJPUT	DAIRY CHEMISTRY DIVISION, NATIONAL DAIRY RESEARCH INSTITUTE, KARNAL, INDIA.
2	SUMANDEEP	DAIRY CHEMISTRY DIVISION, NATIONAL DAIRY RESEARCH INSTITUTE, KARNAL, INDIA.
3	DR. RAJAN SHARMA	DAIRY CHEMISTRY DIVISION, NATIONAL DAIRY RESEARCH INSTITUTE, KARNAL, INDIA.

PCT International Classification Number

H04R 1/10

PCT International Application Number

N/A

PCT International Filing date

PCT Conventions:

#	PCT Application Number	Date of Convention	Priority Country
1			NA