Title of Invention	"A TRANSPORT PROTEIN"
Abstract	H ABSTRACT "A TRANSPORT PROTEIN" The invention relates to a transport protein which can be obtained by nnodifying the heavy chain of the neurotoxin formed by Clostridium botulinum. The protein binds specifically to nerve cells with a higher affinity as the native neurotoxin. The invention also relates to a method for the production of transport protein, the nucleic acids coding for the transport protein, the transport protein containing pharmaceutical and cosmetic compositions and use thereof. i: • - 31 I i

Title of Invention

"A TRANSPORT PROTEIN"

Abstract

H ABSTRACT "A TRANSPORT PROTEIN" The invention relates to a transport protein which can be obtained by nnodifying the heavy chain of the neurotoxin formed by Clostridium botulinum. The protein binds specifically to nerve cells with a higher affinity as the native neurotoxin. The invention also relates to a method for the production of transport protein, the nucleic acids coding for the transport protein, the transport protein containing pharmaceutical and cosmetic compositions and use thereof. i: • - 31 I i

Full Text	-Transport Protein I Used to Interduce\| Compounds into Nerve Cells The present invention relates to a transport protein which binds to neurons, is accommodated by receptor-mediated endocytosis and is translocated from the acid, endosomal compartment into the cytosol of neurons. This protein is used as a transporting means for translocating other chemical substances (e. g. proteases), which are unable physiologically to penetrate into the cytosol of nerve cells through the plasma membrane. The present invention relates to the use of a transport protein for inhibiting the release of neurotransmitters. Nerve cells release transmitter substances by exocytosis. The fusion of the membranes of intracellular vesicles with the plasma membrane is referred to as exocytosis. In the course of this process the vesicular contents is simultaneously discharged into the synaptic gap. The fusion of the two membranes is regulated by calcium, reacting with the protein synaptotagmin. Together with other cofactors synaptotagmin controls the status of three so-called fusion proteins, SNAP-25, synaptobrevin 2 and syntaxin 1A. While syntaxin 1A and synaptobrevin 2 are integrated into the plasma and/or vesicle membrane, SNAP- 25 binds only Bghtfy to the plasma membrane. To the extent that the intracellular calcium concentration increases, the three proteins bind to one another, both membranes approaching one another and subsequently fusing together. In the case of choiinergic neurons acetyl choline is released, causing muscle contractions, perspiration and other cholinergically provoked reactions. The above mentioned fusion proteins are the target molecules (substrates) of the light chains of the clostridial neurotoxins, formed by the bacterium Clostridium botuiinum. The anaerobic, gram-positive bacterium Clostridium botuiinum produces seven different types of protein neurotoxins. The latter are referred to as the Botulinus neurotoxins (BoNT/A to BoNT/G). Among these, in particular BoNT/A and BoNT/B cause a neuroparalytic disorder in humans and animals, referred to as botulism. The spores of Ciostridium botu/inum can be found in the soil, but may also develop in incorrectly sterilised and sealed home-made food preserves, to which many cases of botulism are attributed. BoNT/A is the most lethal of all known biological substances. As little as 5-6 pg of purified BoNT/A represents an MLD (Multiple Low Dose). One unit (Engl.: Unit, U) of BoNT is defined as the MLD, killing half of the female Swiss Webster mice, each weighing 18 - 20 g, after intraperitoneal injection. Seven immunologically different BoNTs were characterised. They are denoted as BoNT/A, B, Ci, D, E, F and G and may be distinguished by neutralisation with serotype-specific antibodies. The different serotypes of BoNTs differ in affected animal species with regard to severity and duration of the paralysis caused. Thus, with regard to paralysis, BoNT/A is 500 times more potent in rats for example, than BoNT/B. In addition, BoNT/B has proved to be non-toxic in primates at a dosage of 480 U/kg of body weight. The same quantity of BoNT/A corresponds to 12 times the lethal dose (LD) of this substance in primates. On the other hand, the duration of paralysis after BoNT/A injection in mice is ten times longer than after injection of BoNT/E. BoNTs have been used clinically for treating neuromuscular disorders, characterised by hyperactivity in skeleton muscles, caused by pathologically overactive peripheral nerves. BoNT/A has been approved by the U.S. Food and Drug Administration for treating blepharo-spasm, strabism and hemi-facial spasms. Compared with BoNT/A the remaining BoNT serotypes are evidently less efficacious and manifest a shorter duration of efficacy. Clinical effects of BoNT/A administered peripheral-intramuscularly are usually noticeable within a week. The duration of symptom suppression by one single intramuscular injection of BoNT/A is normally about 3 months. The clostridial neurotoxins specifically hydrolyse different proteins of the fusion apparatus. BoNT/A, Ci and E split SNAP-25, while BoNT/B, D, F, G as well as tetanus neurotoxin (TeNT) attack the vesicle-associated membrane protein (VAMP) 2 - also referred to as synaptobrevin 2 -. BoNT/Ci furthermore splits syntaxin 1A. The Clostridium bacteria release the neurotoxins as single-chain polypeptides each having 1251 to 1315 amino acids. Thereafter endogenous proteases split each of these proteins at a defined location into 2 chains each ('nicking'), the two chains however remaining interlinked by a disulphide-bridge. These dual-chain proteins are referred to as holo-toxins (see Shone et al. (1985), Eur J Biochem 151, 75-82). The two chains have different functions. While the smaller fragment, the light chain (light chain = LC), represents a Zn2+-dependent endoprotease, the larger unit (heavy chain = HC) represents the transporting means of the light chain. By treating the HC with endopeptidases two 50 kDa fragments were brought about (see Gimenez et al. (1993), J Protein Chem 12, 351-363). The amino-terminal half (Hm-fragment) integrates into membranes at a low pH-value and enables the LC to penetrate into the cytosol of the nerve cell. The carboxyterminal half (He-fragment) binds to complex polysialogangltosides, occurring exclusively in nerve cell membranes and to protein receptors not identified to date (Halpern et al. (1993), Curr Top Microbial Immunol 195, 221-241). The latter explains the high neuroselectivity of the clostridial neurotoxins. Crystalline structures confirm that BoNT/A disposes of three domains, which may be harmonised by the three steps of the action mechanism (see Lacy et al. (1998), Nat Struct Biol 5, 898-902). Moreover, these data give rise to the conclusion that within the He-fragment two autonomous subunits (sub-domains) exist of 25 kDa each. The first proof for the existence of the two functional sub-domains was brought about by the amino-terminal (HCN and the carboxy-terminal half (HCc) of the He-fragment of the TeNT, which were expressed in recombinant form and which revealed that the HCC-, but not the HCN domain binds to neurons (see Herreros et al. (2000), Biochem J 347, 199-204). The protein receptor-binding site of the synaptotagmin was discovered inside the Hcc-domains of BoNT/B and G, proving their separate functionality (see Rummel et al. (2004), J Biol Chem 279, 30865-70). Under physiological conditions the HC binds to neuronal gangliosides, is received inside the cell by receptor-mediated endocytosis and reaches the natural vesicle circulation via the endosomal compartment. In the acid medium of the early endosomes, HN, the amino-terminal half of HC, penetrates into the vesicle membrane and forms a pore. Each substance (X), linked to HC via a disulphide bridge, will be split off the HC by intracellular redox systems, gaining access to the disulphide bridge and reducing it. X will ultimately appear in the cytosol. In the case of the ciostridial neurotoxins the HC is the carrier of an LC, splitting its specific substrate in the cytosol in the final step. The cycle of complex formation and dissociation of the fusion proteins is interrupted and the release of acetyl choline is consequently inhibited. As a result thereof, striated muscles are paralysed and sweat glands cease their secretion. The active period of the individual BoNT serotypes differs and depends on the presence of intact LC in the cytosol. As all neurons possess receptors for ciostridial neurotoxins, it is not only the release of acetyl choline which may be affected, but potentially also the release of the substance P, of noradrenalin, GABA, glycine, endorphin and other transmitters and hormones. That the cholinergic transmission is blocked preferentially, may be explained by the fact that the HC in the periphery enters into the neuron. Central synapses are protected by the blood-brain-barrier, which cannot be surmounted by proteins. The HCs possess a high affinity for peripheral nerve cells, mediated predominantly by the interaction with complex polysialogangliosides - these are glycol lipids composed of more than one sialine acid (see Halpern et al. (1995), Curr Top Microbiol Immunol 195, 221-41). As a result, the LCs binding to them reach only this cell type and become active only in these cells. BoNT/A and B bind merely one molecule ganglioside GT1b each. In order to research the role played by the amino acids, which build the binding pocket, a recombinant He-fragment was produced according to the invention. This technique permits to exchange individual amino acids. Thus, positivelycharged amino acids may be substituted by negatively-charged or neutral amino acids, and vice versa. Slight modifications in the surface of the binding pocket produce no dramatic effect regarding the passing ability of the gangliosides. It could be shown that the affinity receded by more than 99%, if e.g. the amino acid in position 1266, the tryptophane - referred to as W in the SXWY-motive - is substituted by an aliphatic residue, e.g. leucine. However, the contrary has also been observed, The substitution of amino acids, extending into the binding pocket, resulted in an increase of the affinity to gangliosides. Since the configuration of the binding pocket is so decisive for the affinity of the HC to the ganglioside receptor, the proteolytic potency of the associated LC, simultaneously with the affinity of the HC to the ganglioside receptor, either increases or decreases in harmony with the affinity. In a ligand-receptor-study specific amino acid residues were thus characterised according to the invention in the ganglioside-binding pocket of BoNT/A and substituted in order to increase the affinity to the ganglioside receptor accordingly. The affinity of the mutated He-fragment was determined in ganglioside and synaptosome-binding assays. Subsequently, the HC exhibiting the same mutations was coupled to LC-A, for which purpose a thrombin-sensitive amino acid sequence was used. The recombinant protein was activated ('nicked') by thrombin and resulted in a double-chain molecule, both chains being interlinked by a single disulpUide bridge. The activity of the constructs was tested in synaptosomes of rat brain - a preparation releasing transmitters. The extent of transmitter release inhibition was considered as the measure of the degree of activity of the constructs. In addition, the potency of the individual constructs was analysed by means of the isolated nerve-muscle-preparation of the mouse (Hemi-Diaphragma-Assay = HDA), representing the physiological object of clostridial neurotoxins. Disorders and symptoms which are to be treated with TrapoX are accompanied by a focally increased activity of motor neurons and vegetative nerve cells. The increased activity results in painful cramps of the muscles innerved by these cells and in an excessive liquid secretion from gland cells. Furthermore, facial wrinkles occur in different regions due to the increased activity. The cause is a pathologically increased release of acetyl choline from the peripheral nerve ends. If TrapoX is injected into the affected muscle, a relaxation of the affected muscles, the drying up of secretion and smoothing of the facial skin comes about after a latency of 1-3 days. This is due to an inhibition of the release of acetyl choline by TrapoX. The patient becomes virtuaJly pain-free and the pain provoked by the muscle cramp is alleviated and disappears completely. The release of acetyl choline is inhibited both in humans as well as in animals. Animal testing is therefore used routinely both as evidence of BoNT in poisoning cases as well as for activity determination of BoNT-drugs (Botox, Dysport, Xeomin). The activity of BoNT is quantified by performing a determination of the LDso in mice. In this context one determines the dose, killing 50% of the animals of one test group. It is obvious that apart from doses not destroying any animal, doses may be administered killing 100% of the animals from one group. Since the poison is administered systemically (i.p.), a large number of animals thus die painfully of respiratory arrest, caused by a paralysis of the respiratory muscles. In order to avoid animal tests, we have introduced the Mouse Hemi-Diaphragma Assay. With the LD50 test, trial mice die of respiratory paralysis, caused by paralysis of the respiratory muscles. This means that the respiratory muscle, including the innerving nerve (Nervus phrenicus) can be removed from the mouse and be poisoned in vitro. BoNT will bind to its receptors, will enter the cell and be translocated and will finally split its substrate, whereupon the muscle paralyses. There is a strict correlation between the LDso value and the paralysis of the respiratory muscle. This in vitro test represents, as it were, a watereddown version of the animal test (Wohlfarth K, Goeschel H, Frevert J, Dengler R, Bigalke H, Botolinum A toxis: units versus units. Naunyn Schmiedeberg's Arch Pharmacol, 1997 Mar; 335(3):335-40). One can therefore assume that the BoNT, paralysing the diaphragm in vitro, also acts in the living mouse, killing the latter according to the dose administered. This animal test replacement method is so convincing that the Mouse Hemi- Diaphragma-Assay will shortly be accepted for the EU member states by the ED Pharmacopoeia as the official testing method for BoNT. The increased efficacy of TrapoX in the mouse diaphragm preparation thus suggests an increased efficacy in humans as well. In the more recent past, the BoNT/A complex was used for treating motor dystonias, as well as for attenuating excessive sympathetic activity (see Benecke et al. (1995), Akt Neural 22, 209ff) and for alleviating pain and migraine (see Sycha et al. (2004), J Neural 251,19-30). This complex consists of the neurotoxin, various haemagglutinines and a non-toxic, non-haemagglutinating protein. The complex dissociates rapidly at physiological pH. The resultant neurotoxin is the sole ingredient of the complex which is therapeutically relevant and brings about an alleviation of the symptoms. Since the underlying neurological illness is not cured, the complex needs to be injected again at intervals of three to four months. Depending on the quantity of the injected foreign protein, some patients develop specific BoNT/A-antibodies, These patients become resistant to the neurotoxin. Once antigen-sensitive cells have recognised the neurotoxin and antibodies have been formed, the relevant brain cells are conserved over years. For this reason it is important to treat the patient with preparations of the highest possible activity at the lowest possible dosage. The preparations should furthermore not contain any further proteins of bacterial origin, since these may act as immuno-adjuvants. Such substances attract macrophages, which recognise both the immuno-adjuvants as well as the neurotoxins, presenting them to the lymphocytes, which thereupon respond by forming immunoglobulins. Consequently, only products of extreme purity, not containi ng any foreign proteins, may be used for therapy. The present invention now provides a transport protein (Trapo), which is able to overcome the above described problems of the methods known to date. Preferably, a transport protein (Trapo) is provided, the affinity of which to complex; gangliosides is increased at least three fold. "Binding to nerve cells with a higher affinity than native neurotoxin". The native neurotoxin is in this case preferably the native neurotoxin of C. botulinum. Preferably, the native neurotoxin is in this context Botulinus neurotoxin A and/or Botulinus neurotoxin B and/or Botulinus neurotoxin G from C. botulinum. The Botulinu s neurotoxin prepared in recombinant form from £. coli, which, inter alia, contains the amino acid sequence identical to the native Botulinus neurotoxin, acts in a pharmacologically identical manner to the native Botulinus neurotoxin and is referred to as recombinant Botulinus neurotoxin wild type. The nerve cells mentioned in this case are cholinergic motor neurons. Preferably, the transport protein binds specifically to polysialogangliosides on the nerve cell membrane surface, such as e.g. GD1a, GD1b or GT1b. The binding is determined preferably in vitro. Particularly preferably, the determination is performed by the use of a n assay, elucidated in detail in the examples. The term "modification of the heavy chain of the neurotoxin formed by C. botulinum." The amino acid and/or nucleic acid sequence of the heavy chain (HC) of the neurotoxin formed by C, botulinum are generally available from publicly accessible databases, for each of the known serotypes A to G (also refer to table 1). Modification includes in this context that at least one amino acid is deleted, added, is inserted into the amino acid sequence, or that at least one amino acid of the native neurotoxin is substituted by another naturally occurring or not naturally occurring amino acid and/or that one amino acid in the stated amino acid sequence is modified post-translationally. Post-translational modifications include in this context glycosylations, acetylations, acylations, de-aminations, phosphorylisations, isoprenylisations, glycosyl phosphatidyl inositolisations and further modifications known to the person skilled in the art. The HC of the neurotoxin formed by C. botulinum includes three sub-domains, i.e. the amino-terminal 50 kDa-sized translocation domain HN, the 25 kDa HCNdomain following thereon, and the carboxyl-terminally situated 25 kDa Hccdomain. Together, the HCN- and Hcc-domains are denoted as He-fragment. The corresponding amino acid sections of the respective sub-domains for the individual serotypes and their variations are apparent from Table 1. In order to describe in detail hybrid proteins with domains of different BoNT serotypes, the following nomenclature is introduced in what follows. The term scAtAAB denotes e.g. a single-chain neurotoxin (sc), consisting of the four domains LC, HN, HCN and Hcc- each domain, according to its origin, being marked by the capital letter of the respective serotype. This means that scAtAAB is derived from LC, HN and HCN, while the Hcc-domain of BoNT/A was substituted by BoNT/B. The small letter "t" symbolises an inserted thrombin marker sequence between LC and HN. (Table Removed) The present invention relates, in particular, to a transport protein, obtained by modifying the HC of the neurotoxin formed by Clostridium botulinum, the said protein, with a higher affinity than the native neurotoxin, binding specifically to nerve cells and being received by these cells by endocytosis. The transport protein provided in the present invention exhibits an increased specific affinity of its ganglioside-binding domain to complex polysialogangiiosides. The increase of the affinity is preferably attained by substitution or deletion of amino acids. According to a preferred embodiment the transport protein exhibits an affinity which is at least 15% higher than the native neurotoxin. Preferably, the transport protein exhibits an affinity which is at least 50% higher, particularly preferably at least 80% higher and in particular at least 90% higher than the native neurotoxin. According to a preferred embodiment the modification of the HC takes place in the region of the He-fragment of the given neurotoxin. If the modification includes a substitution, deletion, insertion or addition, the latter may also be performed, for example, by specific mutagenesis, methods in this context being known to the person skilled in the art. The amino acids present in the native neurotoxin are in this context modified either by naturally occurring or by not naturally occurring amino acids. Amino acids are, in principle, divided into different physicochemical groups. Aspartate and glutamate belong to the negatively-charged amino acids. Histidine, arginine and lysine belong to the positively-charged amino acids. Aspargine, glutamine, serine, threonine, cysteine and tyrosine belong to the polar amino acids. Glycine, alanine, valine, leucine, isoleucine, methionine, proline, phenylalanine and tryptophane belong to the non polar amino acids. Aromatic side groups are to be found among the amino acids histidine, phenylalanine, tyrosine and tryptophane. In general, it is preferred to substitute an amino acid by a different amino acid pertaining to another physicochemical group. According to a preferred embodiment of the invention, the transport protein is a Botulinus neurotoxin serotype A to G. In a preferred embodiment of the invention, the transport protein is derived from the protein sequence of the clostridium botulinum neurotoxin type A (database no. AAA23262 and CAA51824). A further embodiment of the present invention relates to a transport protein, wherein at least one amino acid in the positions 1117, 1202 to 1204, 1252 to 1254, 1262 to 1267, 1270 and 1278 to 1279 of the protein sequence of the Clostridium botulinum neurotoxin type A (database no. AAA23262 and CAA51824) has either been removed or been substituted by a naturally occurring or not naturally occurring amino acid. A further embodiment of the present invention relates to a transport protein, wherein amino acids in positions 1092 to 1296 of the protein sequence of the Clostridium botulinum neurotoxin type A (database no. AAA23262 and CAA51824) - a region including the ganglioside-binding domain - have been substituted by the sequence of Clostridium botulinum neurotoxin type B protein (database no. AAA23211), amino acids 1079 to 1291, Clostridium botulinum neurotoxin type Ci protein (database no. CAA51313), amino acids 1093 to 1291 Clostridium hotulinum neurotoxin type D protein (database no. CAA38175), amino acids 1080 to 1276, Clostridium hotulinum neurotoxin type E protein (database no. CAA44558), amino acids 1067 to 1252, Clostridium butyricum neurotoxin type E protein (database no. CAA43998), amino acids 1067 to 1251, Clostridium botulinum neurotoxin type F protein (database no. CAA57358), amino acids 1085 to 1278, Clostridium baratii neurotoxin type F protein (database no. CAA48329), amino acids 1076 to 1268, Clostridium botulinum neurotoxin type G protein (database no. CAA52275), amino acids 1087 to 1297. Further Hcc-domains suitable for interchangeability with amino acid positions are apparent from Table 1. A further embodiment of the present invention relates to a composition containing a transport protein according to the invention and at least one intervening molecule (X). The intervening molecule may be a small organic molecule, a peptide or a protein; preferably covalently bonded, e.g. by a peptide-, ester-, ether-, sulphide-, disulphide- or carbon-carbon-bond. In addition, the intervening molecule includes all known therapeutically active substances, Cytostatics, antibiotics, virustatics, but also immunoglobulins are preferred in this context. In order to better utilise the increased affinity of the Trapo, it was bound aminoterminally to an LC of BoNT/A, B, C-i, D, E, F or G via an amino acid sequence, which is specifically recognised and split by the protease thrombin, bringing about a specific TrapoX. The active strength of the said TrapoX, in comparison with native BoNT/A, was increased and particularly preferred by a factor of at least 3. This new product, which is free of foreign proteins, will dramatically reduce the stimulation of the immune systems due to the greater purity of the material and the low dosage. A further embodiment of the present invention relates to a transport protein, wherein the protein is a protease, splitting one or a plurality of proteins of the release apparatus of neurotransmitters, the protease being selected from the group of neurotoxins consisting of the LC of the Clostridium botulinum neurotoxins, in particular of type A, B, d, D, E, F and G or a proteolytically active fragment of the LC of a Clostridium botulinum neurotoxin, in particular a neurotoxin of serotype A, B, CL D, E, F and G, the fragment exhibiting at least 0,01% of the proteolytic activity of the native protease, preferably at least 5%, particularly preferably at least 50%, in particular at least 90%. Preferably, the transport protein and the protease are derived from the same C. botulinum neurotoxin serotype, in particular and preferably the HN-domain of the transport protein and the protease are derived from the C. botulinum neurotoxin serotype A. The sequences of the proteases are generally accessible at databases and the database numbers are apparent from Table 1. The proteolytic activity of the proteases is determined by way of substrate separation kinetics (see Bina et al. (2002), Biochemistry 41 (6), 1717-23). The LCs are characterised in that they contain the sequence His-Glu-Leu-Xaa- His-(Xaa)33.36-Glu(Xaa)84.9o-Glu-(Xaa)ii-Arg-Xaa-Xaa-Tyr, wherein Xaa may be any amino acid. The transport protein of the present invention is characterised in that the protein and the protease stem from the previous groups of proteins and/or proteases. According to a further embodiment of the present invention, a method for producing the transport protein is provided. In this case, in a first step a nucleic acid coding for the transport protein is provided. The coding nucleic acid may represent in this context RNA, DMA or mixtures thereof. The nucleic acid may furthermore be modified with regard to its nuclease resistance, such as e.g. by inserting phosphorthioate bonds. The nucleic acid may be produced from a starting nucleic acid, the latter being accessible e.g. by cloning from genomic or cDNA-databases. Moreover, the nucleic acid may be produced directly by solid phase synthesis. Suitable methods are known to the person skilled in the art. If one assumes a starting nucleic acid, a specific modification, e.g. by localityspecific mutagenesis, may be brought about, resulting in at least one addition, insertion, deletion and/or substitution on the amino acid level. The nucleic acid is then linked operatively to a suitable promoter. Suitable promoters for expression in known expression systems are known to the person skilled in the art. The choice of promoter depends in this case on the expression systems used for expression, in general, constitutive promoters are preferred, but inducible promoters may likewise be used. The construct produced in this manner includes at least one part of a vector, in particular regulatory elements, the vector, for example, being selected from X-derivates, adenoviruses, baculoviruses, vaccinia viruses, SV40-viruses and retroviruses. The vector is preferably capable of expressing the nucleic acid in a given host ceil. The invention further provides host cells, which contain the vector and are suitable for expressing the vector. Numerous prokaryotic and eukaryotic expression systems are known in the state of the art, the host cells being selected, for example, from prokaryotic cells such as E. coli or B. megaterium, from eukaryotic cells such as S. cerevisiae and P. pastoris. Although higher eukaryotic cells, such as insect cells or mammal cells, may be used as well, host cells are nevertheless preferred, which, like C. botulinum, do not possess a glycosylation apparatus. According to a preferred embodiment the nucleic acid codes for the Hcc-domain of the C. botulinum neurotoxin. This nucleic acid contains endonucleaseinterfaces, flanking the nucleic acid coding for the He-fragment, the endonuclease sites being compatible with those of other He-fragments of C. Doiunnum neurotoxins, in order to permit their easy modular substitution in the gene coding for the transport protein, while the similarity of the amino acid sequence is preserved. If a composition according to the invention is provided, which, apart from the transport system, further contains at least one intervening molecule, and this intervening molecule, a peptide or protein, is functionalised either with a carboxyterminal cysteine or a mercapto-group, then, in an analogous manner, as described before, the peptide and/or protein may be produced recombinantly, for example by using binary vectors or various host cells. If the same host cell is used for the expression both of the transport protein and the peptide or protein, an intermolecular disulphide bond is preferably formed in situ. For a more efficient production in the same host cell, the nucleic acid coding for the peptide or protein may also be translated with that of the transport protein in the same reading frame, so that a single-chain polypeptide is produced. In this case preferably an intramolecular disulphide bond is formed in situ. For simple hydrolysis of the likewise present peptide cross-linking between the transport protein and the peptide and/or protein, an amino acid sequence is inserted at the amino-terminus of the transport protein, which is either specifically recognised and separated by the protease thrombin or by a specific endoprotease of the host cell. If this is not possible, an appropriate intermolecular disulphide-linkage, after separate purification of the transport protein and the protein, may subsequently be brought about by oxidation processes known to the person skilled in the art. The peptide or protein may also be obtained directly by synthesis or fragment condensation. Appropriate methods are known to the person skilled in the art. The transport protein and the peptide, or protein respectively, are subsequently purified. For this purpose methods are used, known to the person skilled in the art, such as e.g. chromatography-methods or electrophoresis, A further embodiment of the present invention relates to the pharmaceutical composition, including the transport protein and optionally a pharmaceutically acceptable excipient, a diluent and/or an additive and which is suitable to treat the following disorders or diseases: hemt-facial spasms, spasmodic torticollis, spasticities, dystonias, migraine, pain, disorders of the neck and lumbar vertebral column, strabism, hypersalivation and depressive diseases. The pharmaceutical composition is suitable for oral, intravenous, subcutaneous, intramuscular and topical administration. Intramuscular administration is preferred. A dosing unit of the pharmaceutical composition contains approximately 0,1 pg to 1 mg of transport protein and/or the composition according to the invention. A further embodiment of the present invention includes a cosmetic composition, consisting of the transport protein and a pharmaceutical excipient, a diluent and/or an additive, suitable for treating hyperhydrosis and very pronounced facial wrinkles. The cosmetic composition is suitable for oral, intravenous, subcutaneous, intramuscular and topical administration. Intramuscular administration is preferred. A dosing unit of the cosmetic composition contains about 0,1 pg to 1 mg of transport protein and/or the composition according to the invention. The cosmetic composition is suitable to treat hyperhydrosis and facial wrinkles. The transport protein described in the present invention may be produced by a suitable host cell, such as e.g. Escherichia coli, Saccharomyces cerevisiae, Pichia pastohs or Bacillus megaterium, which multiplies a recombinant expression vector, the vector coding for a transport protein. The present invention is elucidated by the accompanying drawings, wherein: Figure 1 shows that the affinity of the mutated He-fragment of BoNT/A to synaptosome membrane preparations from the rat brain is three times higher than that of the H c-fragment of the wild type of BoNT/A. Figure 2 shows t he binding of different BoNT/A He-fragment mutants to rat brain synaptosomes, the affinity of the BoNT/A He-fragment wild type being set to 100% as a standard. The first column shows the affinities of the BoNT/A mutants, showing mutations of the amino acids Y1117 resulting in an increase. The second column shows further BoNT/A-mutants. The third column shows the affinities of BoN T/A-mutants exhibiting double mutations, which enhance the binding to nerve cell membranes (synaptosomes). Figure 3 shows t he increased neurotoxicity of the Y1117A-mutant of BoNT/A in comparison with the BoNT/A-wild type on the isolated nervus phrenicus - diaphragm musd e-preparation of the mouse. Figure 4 shows the binding of the four BoNT/A He-fragment hybrids HcAB, HcAC, HcAE and McAT (T = tetanus neurotoxin} in nerve cell membranes (synaptosomes), the BoNT/A He-fragment wild type being set to 100% as a standard. Figure 5 shows the increased neurotoxicity of the total toxin hybrids consisting of BoNT/A and either the He-fragment or the Hcc-domain of BoNT/B in comparison with the BoNT/A-wiid type in the isolated nervus phrenicus - diaphragm musclepreparation of the mouse. In detail, the present invention contains a transport protein (Trapo), formed by a modification of the HC of the neurotoxin produced by Clostridium botulinum, preferably specifically binding to neurons, accommodated intracellulariy by receptor-mediated endocytosis and translocated from the acid endosomal compartment into the cytosol of neurons. This protein is used as a transporting means in order to introduce into the cells proteases and other substances bound to the said transporting means, unable to penetrate physiologically into the plasma membrane and to reach the cytosol of nerve cells. The substrates of the proteases are intracellularly localised proteins and peptides participating in the transmitter release. After separation of the substrates, the specific functions of the neurons are blocked; the cells themselves are not damaged. One of these functions is exocytosis, bringing about the neurotransmitter release. If the release of transmitters is inhibited, the transmission of signals from cell to cell is blocked. For example, striated muscles are paralysed if the release of acetyl choline is inhibited at the neuromuscular contact point. This effect may be used therapeutically, if TrapoX is applied to nerve ends of spastic or dystonic muscles. Other active substances are, for example, substances exhibiting anti-viral action- Conjugated with Trapo, they might be of use for treating viral infections of the nervous system. The present invention also relates to the use of a transport protein for inhibiting the release of neurotransmitters. If patients are treated with the native forms of BoNT/A and B, injection of these non-human proteins, despite the low dosage, causes the formation of antibodies, so that the therapy must be stopped in order to prevent anaphylactic shock. By applying a substance with the same active mechanism having a higher transport efficiency of the enzymatic activity, the dosage may be lowered drastically and the formation of antibodies will not occur. These properties are attributed to the transport protein described herein. Although examples are stated for application, the suitable mode of application and the dosage is, in general, individually determined by the treating physician. Such decisions are routinely made by each physician well versed in the relevant special field. Thus, the mode of application and the dosage of the neurotoxin may e.g. be selected in accordance with the invention described herein, based on criteria such as the solubility of the selected neurotoxin or the intensity of the pain to be treated. The treatment interval for native BoNT/A and B is currently three to four months on average. Prolonging this interval would reduce the risk of the formation of antibodies and allow a longer treatment period with BoNT. The increase of LC in the cytosol would extend its decomposition timewise and would thus also prolong the duration of action. The transport protein described here exhibits a higher affinity and reception rate than the native HC-A. The following example merely serves for elucidation and should not be contemplated in a limiting manner. Examples Recombinant expression of the genetically engineered TrapoX in E. coli The DMA-sequence of the HC of BoNT/A was amplified in chromosomal DNA of Clostridium botulinum (database no. AAA23262) by means of PCR. For this purpose, by means of specific oMgonucleotides, the codon for the ami no acid tyrosine 1117 was substituted by a base triplet coding for the amino acid residue of alanine. Furthermore, the 5'-end of the gene was supplemented by a DNAsequence, coding for the amino acids of the recognition sequence of thrombin. This DMA-sequence was inserted into a bacterial expression vector. The inserted gene for Trapo was in this case fused with an oligonucleotide at the 3'-end, coding for a carboxy-terminal affinity peptide such as e.g. the Strep-day, 6xHNday or Hise-day. The expression vector pAR-Trapo produced in this manner is the starting basis for adding carrier molecules, such as the LC of the BoNT. The DNA-sequence of the LC of BoNT/A was amplified by the PCR-method in the chromosomal DNA-sequence of Clostridium botulinum (database no. AAA23262) and inserted into the expression vector pAR-Trapo upstream of the coded thrombin recognition sequence. The expression vector pAR-TrapoX thus produced was transformed into an E.coli K12 strain and the expression of the protein TrapoX was induced under the conditions of Biological Safety Level 2 and in compliance with the rules and regulations issued for the project by the district government of Hanover, file reference 501g.40654/3/57/3. The over-expressed TrapoX was isolated in an affinity-chromatographic manner, following the directions of the manufacturer, as a single-chain protein with a molecular weight of 150 kDa. The protein was subsequently hydrolysed with thrombin conjugated on sepharose bringing about a pure protein, the two chains of which remained interlinked by a disulphide-bridge. Compared with the wild type of BoNT/A, this protein exhibited an affinity, increased by 300%, to isolated ganglioside GT1b immobilised on micro titre plates and to synaptosome membrane preparations from rat brain (Figure 1). The catalytic activity of the LC-A was not changed, as has been shown in the in vitro splitting of recombinant SNAP-25. The potency of the TrapoX with regard to inhibition of the neurotransmitter release in functional synaptosomes from rat brain had increased by 300%, compared with the native BoNT/A recovered from Clostridium batulinum. In nerve muscle-preparations of the mouse (HDA), the potency of the TrapoX was likewise increased by 300% compared with the native BoNT/A (Figure 2). Measurement of the binding to rat brain svnaptosomes and the neurotoxicitv in the HDA of different BoNT/A-mutants The binding of radioactively marked He-fragments to rat synaptosomes was measured as stated in Rummel et al., J. Mol. Biol. 326 (2003), 835-847. The neurotoxictty of the BoNT/A-mutants was determined as described by Habermann et al., Naunyn Schmiedeberg's Arch. Pharmacol. 311 (1980), 33-40. The comparison of the binding of different BoNT/A-mutants as compared with the wild type is shown in the following table: (Table Removed) The mutation of individual determined amino acids within the ganglioside binding pocket of BoNT/A resulted in an increase of the binding to nerve cells. Preferably, in position 1117, tyrosine is substituted by alanine, cysteine or valine. In particular, the substitution of the tyrosine residue in position 1117 by alanine results in an increase of the affinity to about 330%. Further mutations of individual amino acids from the ganglioside binding pocket in position 1252 and 1253 result likewise in an increase of the binding. In particular, the mutation of F1252 in tyrosine and H1253 in lysine resulted in an increase of the affinity by 110%, and 50% respectively. Furthermore, increases of the binding to nerve cells can be expected in mutations in positions 1202,1262, 1270, 1278 and 1279. Moreover, mutants of BoNT/A were also tested with double mutations, in which case, in particular, the mutants Y1117C/H1253K and Y1117V/H1253K resulted in an increase of the binding to synaptosomes (cf. Figure 2). It was furthermore determined that the increase of the binding, particularly of the mutant Y1117A of BoNT/A resulted in an increase of the neurotoxicity in the N.phrenicus - neurotoxicity assay (HDA-Assay) (Figure 3). Determination of binding and neurotoxicity of BoNT/A Hy.-hybrids The determination of the binding and the neurotoxicity was performed as described above. The results are reflected in the following table and further in Figures 4 and 5. (Table Removed) Substitution of the Hcc-domain of BoNT/A by the other serotypes, in particular C. botulinum neurotoxin B and C. botulinum neurotoxin C, resulted in an increase of the binding to nerve cells. It was furthermore observed that the substitution of the Hcc-domain of He-fragment of BoNT/A by the corresponding domain of tetanus neurotoxin likewise resulted in an increase of the affinity in nerve cells. The affinity changes also apply to the substitution of the Hcc-domain in the entire BoNT/A. Figure 5 shows in this context that in a hybrid scAtAAB the increase of affinity has a similar effect on increased neurotoxicity. If, instead of the Hccdomain, the entire He-fragment scAtAAB is substituted, corresponding results are observed. In particular, it was observed that an improvement of the neurotoxicity by about 350% was noted when substituting the Hcc-domain or the He-fragment of BoNT/A by that of BoNT/B. Determination of binding of the BoNT-mutants to the ganglioside GT1b Ganglioside GT1b [NAcNeua3Gal(J3NAcGal fi4(NAcNeua8NAcNeua3)GalB4Glc(X] (Sigma-Aldrich) is dissolved in methanol and applied to high-affinity 96-cup polystyrene-micro titre plates (Corning; 1ug GT1b in 100 ul/cup) or, in the case of competition assays to high-affinity CS single fracture strip plates with 125l-BoNTs (Greiner Bio-ohne; 0,1 ug GT1b in 100 Ml / cup). The solvent is evaporated at room temperature and the cups are rinsed three times with a binding buffer (10mM Tris-HCI, 10mM Na2HPO4, 0,5% BSA, pH7,2). The non specific binding sites are then blocked by incubation for two hours in PBS/Tween [140 mM NaCI, 7 mM KCI, 10 mM Na2HPO4, 1,8 mM KH2PO4, 0,05% (VA/) Tween 20, pH 7,2], supplemented by 3% (w/v) BSA. The binding assays are carried out in binding buffers (100ul / cup) for 2 hours at room temperature either with increasing quantities of the wild type or specific quantities of the mutants. Unbound protein is removed in 3 rinsing steps, each with 250ul PBS/Tween buffer. Bound He-fragments are identified by incubation with Strep Tactin conjugated with alkaline phosphatase (ST-AP, IBA GmbH) in a binding buffer for a duration of 2 hours at room temperature according to manufacturer's instructions, p-nitrophenyl phosphate (1 mg/ml in 100 mM glycine, 1 mM MgCb, 1 mM ZnCb, pH 10,4), which ultimately serves as substrate for the alkaline phosphatase. The desphorphoryiation reaction is stopped by adding a 3 M NaOH solution and the extinction is measured at 405 nm using a Spectra Count micro plate reading device (Packard). The competition assays are performed over a period of 2 hours at room temperature in a 100ul binding buffer with 700000cpm/cup [1251]-BoNT, different quantities of native BoNT or recombinant He-fragment. After incubation and removal of the supernatants the cups are rinsed three times with PBS/Tween buffer, dried and separated. The quantities of bound radioactiveiy marked BoNT are then determined in an automatic y-counter (Wallac 1480 Wizard 3). ^ WE CLAIM: 1. A transport protein, obtainable by modification of the heavy chain of the neurotoxin fornied by Clostridium botulinum, wherein the neurotoxin is botulinum neurotoxin type A (BoNT/A), and wherein the transport protein binds to nerve cells with a higher affinity than the native neurotoxin, wherein at least one amino acid in the positions 1117, 1252, 1253, 1270, and 1278 to 1279 of the botulinum neurotoxin type A protein sequence is removed or substituted, either by an amino acid which is naturally occurring or by an amino acid which is of non-natural origin. • 2. A transport protein, obtainable by modification of the heavy chain of the neurotoxin formed by Clostridium botulinum, wherein the neurotoxin is botulinum neurotoxin type A (BoNT/A), wherein the transport protein binds to nerve cells with higher affinity than the native neurotoxin, and wherein the amino acids 1092 to 1296 of Clostridium botulinum neurotoxm type A, which contain the ganglioside-binding domain, are substituted with one of the following sequences: (a) Clostridium botulinum neurotoxin type B protein, amino acids 1079 to 1291, or (b) Clostridium botulinum neurotoxin type Ci protein, amino acids 1093 to 1291, or (c) Hcc-domain of Clostridium tetani neurotoxin proteins. 3. A transport protein, obtainable by modification of the heavy chain of the neurotoxin formed by Clostridium botulinum, wherein the neurotoxin is botulinum neurotoxin type A (BoNT/A), wherein the transport protein binds to nerve cells with higher affinity than the native neurotoxin, and wherein the complete He-fragment consisting of amino acids 867 to 1296 of BoNT/A is substituted by the He-fragment consisting of amino acids 866 to 1291 of BoNT/B. 4. The transport protein as claimed in any one of claims 1 to 3, wherein the protein binds specifically to nerve cells and enters the cells by endocytosis. 27 1 ^ 5. The transport protein as claimed in any one of claims 1 to 3, wherein the protein binds specifically to complex gangliosides of cholinergic motor neurons, localised in the plasma membrane, preferably GT1b. 6. The transport protein as claimed in any one of claims 1 to 5, wherein the protein exhibits an affinity that is at least 15% higher than the native neurotoxin, preferably at least 50% higher, particularly preferably at least 80% higher, in particular at least 90% higher. 7. The transport protein as claimed in claim 1, wherein the amino acid in position 1117 of the botulinum neurotoxin type A protein sequences has been removed or substituted by an amino acid, either occurring naturally or of non natural origin. 8. The transport protein as claimed in claim 7, wherein the substituted amino acid is either alanine, cysteine, glutamate, phenyl alanine, isoleucine, leucine, methionine, asparagine, proline, glutamine, serine, threonine or valine. 9. The transport protein as claimed in claim 8, wherein the substituted amino acid is alanine, cysteine or valine. 10. The transport protein as claimed in claim 1, wherein the amino acid of the botulinum neurotoxin type A protein sequences in position 1252 of the botulinum neurotoxin type A protein sequences is substituted by tyrosine or in position 1253 by lysine. 11. The transport protein as claimed in claim 1, wherein two or three amino acids selected from the positions 1117, 1252, 1253, 1270 and 1278 to 1279 of the botulinum neurotoxin type A protein sequences are removed or substituted. 12. The transport protein as claimed in claim 11, wherein two or three amino acids at positions 1117/1252, 1117/1253, 1117/1262, 1117/1278, 1117/1279 or 1117/1252/1253 are removed or substituted. 13. The transport protein as claimed in claim 11 or 12, wherein said two or three substituted amino acids are selected from the group consisting of 28 •>• Y1117A/F1252Y, Y1117A/H1253K, Y1117AA/1262I, Y1117A/L1278H, Y1117A/G1279N, Y1117C/F1252Y, Y1117C/H1253K, Y1117CA/1262I, Y1117C/L1278H, Y1117C/G1279N, Y1117V/F1252Y, Y1117V/H1253K, Y1117VA/1262I, Y1117V/L1278H, Y1117V/G1279N, Y1117A/F1252Y/H1253K, Y1117C/F1252Y/H1253K and Y1117V/F1252/H1253K. 14. A transport protein as claimed in any one of claims 1 to 13 covalently bonded to at least one intervening molecule, wherein the intervening molecule is a small organic molecule, a peptide, or a protein. 15. The transport protein as claimed in claim 14, wherein the intervening molecule is covalently bonded to the transport protein by a peptide-bond, ester-bond, ether-bond sulphide-bond, disulphide-bond or a carbon-carbon bond. 16. The transport protein as claimed in claim 14, wherein the small organic molecule is a virustatic, a cytostatic, an antibiotic or an immunoglobulin. 17. The transport protein as claimed in claim 14, wherein the intervening molecule is a protein that comprises a protease. 18. The transport protein as claimed in claim 17, wherein the protease comprises a neurotoxin protein of Clostridium botulinum type A, B, Ci, D, E, F or G. 19. The transport protein as claimed in claim 18, characterised in that said transport protein contains the secljuence His-Glu-Leu-Xaa-His-(Xaa)33.35-Glu- (Xaa)84-9o-Glu-(Xaa)ii-Arg-Xaa-Xaa-Tyr, wherein Xaa may be any amino acid. 20. The transport protein as claimed in claim 17, wherein the protease contains a proteolytically active fragment of the neurotoxin of Clostridium botulinum type A, B,Ci,D, E,ForG. 21. The transport protein as claimed in claim 20, characterised in that said transport protein contains the sequence His-Glu-Leu-Xaa-His-(Xaa)33.35-Glu- (Xaa)84-9o-Glu-(Xaa)ii-Arg-Xaa-Xaa-Tyr, wherein Xaa may be any amino acid. 29 9 22. The transport protein as claimed in any one of claims 17 to 21, wherein the protease and the transport protein are covalently bonded by an amino acid sequence, which is specifically recognised and cleaved by an endopeptidase. 23. The transport protein as claimed in claim 22, wherein after cleavage by the endopeptidase a disulphide-bridge interlinks the protease and the transport protein, which, in turn, results in the formation of an active holotoxin. 24. A host cell containing a recombinant expression vector, the expression vector coding for a transport protein as claimed in any one of claims 1 to 23, wherein the host cell is a cell of Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris or Bacillus megaterium. 25. An expression vector, wherein the vector includes a nucleic acid, coding for a transport protein as claimed in any one of claims 1 to 23. Dated this 25**^ day of March 2007 \^^: J r a r i KUMARI OF K & SFARTNERS AGENT FOR THE APPLICANT(S) ; i 30

Full Text

-Transport Protein I Used to Interduce| Compounds into Nerve Cells
The present invention relates to a transport protein which binds to neurons, is
accommodated by receptor-mediated endocytosis and is translocated from the
acid, endosomal compartment into the cytosol of neurons. This protein is used as
a transporting means for translocating other chemical substances (e. g.
proteases), which are unable physiologically to penetrate into the cytosol of
nerve cells through the plasma membrane. The present invention relates to the
use of a transport protein for inhibiting the release of neurotransmitters.
Nerve cells release transmitter substances by exocytosis. The fusion of the
membranes of intracellular vesicles with the plasma membrane is referred to as
exocytosis. In the course of this process the vesicular contents is simultaneously
discharged into the synaptic gap. The fusion of the two membranes is regulated
by calcium, reacting with the protein synaptotagmin. Together with other cofactors
synaptotagmin controls the status of three so-called fusion proteins,
SNAP-25, synaptobrevin 2 and syntaxin 1A. While syntaxin 1A and
synaptobrevin 2 are integrated into the plasma and/or vesicle membrane, SNAP-
25 binds only Bghtfy to the plasma membrane. To the extent that the intracellular
calcium concentration increases, the three proteins bind to one another, both
membranes approaching one another and subsequently fusing together. In the
case of choiinergic neurons acetyl choline is released, causing muscle
contractions, perspiration and other cholinergically provoked reactions.
The above mentioned fusion proteins are the target molecules (substrates) of the
light chains of the clostridial neurotoxins, formed by the bacterium Clostridium
botuiinum.
The anaerobic, gram-positive bacterium Clostridium botuiinum produces seven
different types of protein neurotoxins. The latter are referred to as the Botulinus
neurotoxins (BoNT/A to BoNT/G). Among these, in particular BoNT/A and
BoNT/B cause a neuroparalytic disorder in humans and animals, referred to as
botulism. The spores of Ciostridium botu/inum can be found in the soil, but may
also develop in incorrectly sterilised and sealed home-made food preserves, to
which many cases of botulism are attributed.
BoNT/A is the most lethal of all known biological substances. As little as 5-6 pg of
purified BoNT/A represents an MLD (Multiple Low Dose). One unit (Engl.: Unit,
U) of BoNT is defined as the MLD, killing half of the female Swiss Webster mice,
each weighing 18 - 20 g, after intraperitoneal injection. Seven immunologically
different BoNTs were characterised. They are denoted as BoNT/A, B, Ci, D, E, F
and G and may be distinguished by neutralisation with serotype-specific
antibodies. The different serotypes of BoNTs differ in affected animal species
with regard to severity and duration of the paralysis caused. Thus, with regard to
paralysis, BoNT/A is 500 times more potent in rats for example, than BoNT/B. In
addition, BoNT/B has proved to be non-toxic in primates at a dosage of 480 U/kg
of body weight. The same quantity of BoNT/A corresponds to 12 times the lethal
dose (LD) of this substance in primates. On the other hand, the duration of
paralysis after BoNT/A injection in mice is ten times longer than after injection of
BoNT/E.
BoNTs have been used clinically for treating neuromuscular disorders,
characterised by hyperactivity in skeleton muscles, caused by pathologically
overactive peripheral nerves. BoNT/A has been approved by the U.S. Food and
Drug Administration for treating blepharo-spasm, strabism and hemi-facial
spasms. Compared with BoNT/A the remaining BoNT serotypes are evidently
less efficacious and manifest a shorter duration of efficacy. Clinical effects of
BoNT/A administered peripheral-intramuscularly are usually noticeable within a
week. The duration of symptom suppression by one single intramuscular
injection of BoNT/A is normally about 3 months.
The clostridial neurotoxins specifically hydrolyse different proteins of the fusion
apparatus. BoNT/A, Ci and E split SNAP-25, while BoNT/B, D, F, G as well as
tetanus neurotoxin (TeNT) attack the vesicle-associated membrane protein
(VAMP) 2 - also referred to as synaptobrevin 2 -. BoNT/Ci furthermore splits
syntaxin 1A.
The Clostridium bacteria release the neurotoxins as single-chain polypeptides
each having 1251 to 1315 amino acids. Thereafter endogenous proteases split
each of these proteins at a defined location into 2 chains each ('nicking'), the two
chains however remaining interlinked by a disulphide-bridge. These dual-chain
proteins are referred to as holo-toxins (see Shone et al. (1985), Eur J Biochem
151, 75-82). The two chains have different functions. While the smaller fragment,
the light chain (light chain = LC), represents a Zn2+-dependent endoprotease, the
larger unit (heavy chain = HC) represents the transporting means of the light
chain. By treating the HC with endopeptidases two 50 kDa fragments were
brought about (see Gimenez et al. (1993), J Protein Chem 12, 351-363). The
amino-terminal half (Hm-fragment) integrates into membranes at a low pH-value
and enables the LC to penetrate into the cytosol of the nerve cell. The carboxyterminal
half (He-fragment) binds to complex polysialogangltosides, occurring
exclusively in nerve cell membranes and to protein receptors not identified to
date (Halpern et al. (1993), Curr Top Microbial Immunol 195, 221-241). The
latter explains the high neuroselectivity of the clostridial neurotoxins. Crystalline
structures confirm that BoNT/A disposes of three domains, which may be
harmonised by the three steps of the action mechanism (see Lacy et al. (1998),
Nat Struct Biol 5, 898-902). Moreover, these data give rise to the conclusion that
within the He-fragment two autonomous subunits (sub-domains) exist of 25 kDa
each. The first proof for the existence of the two functional sub-domains was
brought about by the amino-terminal (HCN and the carboxy-terminal half (HCc) of
the He-fragment of the TeNT, which were expressed in recombinant form and
which revealed that the HCC-, but not the HCN domain binds to neurons (see
Herreros et al. (2000), Biochem J 347, 199-204). The protein receptor-binding
site of the synaptotagmin was discovered inside the Hcc-domains of BoNT/B and
G, proving their separate functionality (see Rummel et al. (2004), J Biol Chem
279, 30865-70).
Under physiological conditions the HC binds to neuronal gangliosides, is
received inside the cell by receptor-mediated endocytosis and reaches the
natural vesicle circulation via the endosomal compartment. In the acid medium of
the early endosomes, HN, the amino-terminal half of HC, penetrates into the
vesicle membrane and forms a pore. Each substance (X), linked to HC via a
disulphide bridge, will be split off the HC by intracellular redox systems, gaining
access to the disulphide bridge and reducing it. X will ultimately appear in the
cytosol.
In the case of the ciostridial neurotoxins the HC is the carrier of an LC, splitting
its specific substrate in the cytosol in the final step. The cycle of complex
formation and dissociation of the fusion proteins is interrupted and the release of
acetyl choline is consequently inhibited. As a result thereof, striated muscles are
paralysed and sweat glands cease their secretion. The active period of the
individual BoNT serotypes differs and depends on the presence of intact LC in
the cytosol. As all neurons possess receptors for ciostridial neurotoxins, it is not
only the release of acetyl choline which may be affected, but potentially also the
release of the substance P, of noradrenalin, GABA, glycine, endorphin and other
transmitters and hormones.
That the cholinergic transmission is blocked preferentially, may be explained by
the fact that the HC in the periphery enters into the neuron. Central synapses are
protected by the blood-brain-barrier, which cannot be surmounted by proteins.
The HCs possess a high affinity for peripheral nerve cells, mediated
predominantly by the interaction with complex polysialogangliosides - these are
glycol lipids composed of more than one sialine acid (see Halpern et al. (1995),
Curr Top Microbiol Immunol 195, 221-41). As a result, the LCs binding to them
reach only this cell type and become active only in these cells. BoNT/A and B
bind merely one molecule ganglioside GT1b each.
In order to research the role played by the amino acids, which build the binding
pocket, a recombinant He-fragment was produced according to the invention.
This technique permits to exchange individual amino acids. Thus, positivelycharged
amino acids may be substituted by negatively-charged or neutral amino
acids, and vice versa. Slight modifications in the surface of the binding pocket
produce no dramatic effect regarding the passing ability of the gangliosides. It
could be shown that the affinity receded by more than 99%, if e.g. the amino acid
in position 1266, the tryptophane - referred to as W in the SXWY-motive - is
substituted by an aliphatic residue, e.g. leucine. However, the contrary has also
been observed, The substitution of amino acids, extending into the binding
pocket, resulted in an increase of the affinity to gangliosides. Since the
configuration of the binding pocket is so decisive for the affinity of the HC to the
ganglioside receptor, the proteolytic potency of the associated LC,
simultaneously with the affinity of the HC to the ganglioside receptor, either
increases or decreases in harmony with the affinity.
In a ligand-receptor-study specific amino acid residues were thus characterised
according to the invention in the ganglioside-binding pocket of BoNT/A and
substituted in order to increase the affinity to the ganglioside receptor accordingly.
The affinity of the mutated He-fragment was determined in ganglioside and
synaptosome-binding assays. Subsequently, the HC exhibiting the same
mutations was coupled to LC-A, for which purpose a thrombin-sensitive amino
acid sequence was used. The recombinant protein was activated ('nicked') by
thrombin and resulted in a double-chain molecule, both chains being interlinked
by a single disulpUide bridge. The activity of the constructs was tested in
synaptosomes of rat brain - a preparation releasing transmitters. The extent of
transmitter release inhibition was considered as the measure of the degree of
activity of the constructs. In addition, the potency of the individual constructs was
analysed by means of the isolated nerve-muscle-preparation of the mouse
(Hemi-Diaphragma-Assay = HDA), representing the physiological object of
clostridial neurotoxins.
Disorders and symptoms which are to be treated with TrapoX are accompanied
by a focally increased activity of motor neurons and vegetative nerve cells. The
increased activity results in painful cramps of the muscles innerved by these cells
and in an excessive liquid secretion from gland cells. Furthermore, facial wrinkles
occur in different regions due to the increased activity. The cause is a
pathologically increased release of acetyl choline from the peripheral nerve ends.
If TrapoX is injected into the affected muscle, a relaxation of the affected muscles,
the drying up of secretion and smoothing of the facial skin comes about after a
latency of 1-3 days. This is due to an inhibition of the release of acetyl choline by
TrapoX. The patient becomes virtuaJly pain-free and the pain provoked by the
muscle cramp is alleviated and disappears completely.
The release of acetyl choline is inhibited both in humans as well as in animals.
Animal testing is therefore used routinely both as evidence of BoNT in poisoning
cases as well as for activity determination of BoNT-drugs (Botox, Dysport,
Xeomin). The activity of BoNT is quantified by performing a determination of the
LDso in mice. In this context one determines the dose, killing 50% of the animals
of one test group. It is obvious that apart from doses not destroying any animal,
doses may be administered killing 100% of the animals from one group. Since
the poison is administered systemically (i.p.), a large number of animals thus die
painfully of respiratory arrest, caused by a paralysis of the respiratory muscles. In
order to avoid animal tests, we have introduced the Mouse Hemi-Diaphragma
Assay. With the LD50 test, trial mice die of respiratory paralysis, caused by
paralysis of the respiratory muscles. This means that the respiratory muscle,
including the innerving nerve (Nervus phrenicus) can be removed from the
mouse and be poisoned in vitro. BoNT will bind to its receptors, will enter the cell
and be translocated and will finally split its substrate, whereupon the muscle
paralyses. There is a strict correlation between the LDso value and the paralysis
of the respiratory muscle. This in vitro test represents, as it were, a watereddown
version of the animal test (Wohlfarth K, Goeschel H, Frevert J, Dengler R,
Bigalke H, Botolinum A toxis: units versus units. Naunyn Schmiedeberg's Arch
Pharmacol, 1997 Mar; 335(3):335-40).
One can therefore assume that the BoNT, paralysing the diaphragm in vitro, also
acts in the living mouse, killing the latter according to the dose administered. This
animal test replacement method is so convincing that the Mouse Hemi-
Diaphragma-Assay will shortly be accepted for the EU member states by the ED
Pharmacopoeia as the official testing method for BoNT. The increased efficacy of
TrapoX in the mouse diaphragm preparation thus suggests an increased efficacy
in humans as well.
In the more recent past, the BoNT/A complex was used for treating motor
dystonias, as well as for attenuating excessive sympathetic activity (see Benecke
et al. (1995), Akt Neural 22, 209ff) and for alleviating pain and migraine (see
Sycha et al. (2004), J Neural 251,19-30). This complex consists of the
neurotoxin, various haemagglutinines and a non-toxic, non-haemagglutinating
protein. The complex dissociates rapidly at physiological pH. The resultant
neurotoxin is the sole ingredient of the complex which is therapeutically relevant
and brings about an alleviation of the symptoms. Since the underlying
neurological illness is not cured, the complex needs to be injected again at
intervals of three to four months. Depending on the quantity of the injected
foreign protein, some patients develop specific BoNT/A-antibodies, These
patients become resistant to the neurotoxin. Once antigen-sensitive cells have
recognised the neurotoxin and antibodies have been formed, the relevant brain
cells are conserved over years. For this reason it is important to treat the patient
with preparations of the highest possible activity at the lowest possible dosage.
The preparations should furthermore not contain any further proteins of bacterial
origin, since these may act as immuno-adjuvants. Such substances attract
macrophages, which recognise both the immuno-adjuvants as well as the
neurotoxins, presenting them to the lymphocytes, which thereupon respond by
forming immunoglobulins. Consequently, only products of extreme purity, not
containi ng any foreign proteins, may be used for therapy.
The present invention now provides a transport protein (Trapo), which is able to
overcome the above described problems of the methods known to date.
Preferably, a transport protein (Trapo) is provided, the affinity of which to
complex; gangliosides is increased at least three fold.
"Binding to nerve cells with a higher affinity than native neurotoxin". The native
neurotoxin is in this case preferably the native neurotoxin of C. botulinum.
Preferably, the native neurotoxin is in this context Botulinus neurotoxin A and/or
Botulinus neurotoxin B and/or Botulinus neurotoxin G from C. botulinum. The
Botulinu s neurotoxin prepared in recombinant form from £. coli, which, inter alia,
contains the amino acid sequence identical to the native Botulinus neurotoxin,
acts in a pharmacologically identical manner to the native Botulinus neurotoxin
and is referred to as recombinant Botulinus neurotoxin wild type. The nerve cells
mentioned in this case are cholinergic motor neurons. Preferably, the transport
protein binds specifically to polysialogangliosides on the nerve cell membrane
surface, such as e.g. GD1a, GD1b or GT1b. The binding is determined
preferably in vitro. Particularly preferably, the determination is performed by the
use of a n assay, elucidated in detail in the examples.
The term "modification of the heavy chain of the neurotoxin formed by C.
botulinum." The amino acid and/or nucleic acid sequence of the heavy chain (HC)
of the neurotoxin formed by C, botulinum are generally available from publicly
accessible databases, for each of the known serotypes A to G (also refer to table
1). Modification includes in this context that at least one amino acid is deleted,
added, is inserted into the amino acid sequence, or that at least one amino acid
of the native neurotoxin is substituted by another naturally occurring or not
naturally occurring amino acid and/or that one amino acid in the stated amino
acid sequence is modified post-translationally. Post-translational modifications
include in this context glycosylations, acetylations, acylations, de-aminations,
phosphorylisations, isoprenylisations, glycosyl phosphatidyl inositolisations and
further modifications known to the person skilled in the art.
The HC of the neurotoxin formed by C. botulinum includes three sub-domains, i.e.
the amino-terminal 50 kDa-sized translocation domain HN, the 25 kDa HCNdomain
following thereon, and the carboxyl-terminally situated 25 kDa Hccdomain.
Together, the HCN- and Hcc-domains are denoted as He-fragment. The
corresponding amino acid sections of the respective sub-domains for the
individual serotypes and their variations are apparent from Table 1.
In order to describe in detail hybrid proteins with domains of different BoNT
serotypes, the following nomenclature is introduced in what follows. The term
scAtAAB denotes e.g. a single-chain neurotoxin (sc), consisting of the four
domains LC, HN, HCN and Hcc- each domain, according to its origin, being
marked by the capital letter of the respective serotype. This means that scAtAAB
is derived from LC, HN and HCN, while the Hcc-domain of BoNT/A was substituted
by BoNT/B. The small letter "t" symbolises an inserted thrombin marker
sequence between LC and HN.
(Table Removed)
The present invention relates, in particular, to a transport protein, obtained by
modifying the HC of the neurotoxin formed by Clostridium botulinum, the said
protein, with a higher affinity than the native neurotoxin, binding specifically to
nerve cells and being received by these cells by endocytosis.
The transport protein provided in the present invention exhibits an increased
specific affinity of its ganglioside-binding domain to complex
polysialogangiiosides. The increase of the affinity is preferably attained by
substitution or deletion of amino acids.
According to a preferred embodiment the transport protein exhibits an affinity
which is at least 15% higher than the native neurotoxin. Preferably, the transport
protein exhibits an affinity which is at least 50% higher, particularly preferably at
least 80% higher and in particular at least 90% higher than the native neurotoxin.
According to a preferred embodiment the modification of the HC takes place in
the region of the He-fragment of the given neurotoxin. If the modification includes
a substitution, deletion, insertion or addition, the latter may also be performed, for
example, by specific mutagenesis, methods in this context being known to the
person skilled in the art. The amino acids present in the native neurotoxin are in
this context modified either by naturally occurring or by not naturally occurring
amino acids. Amino acids are, in principle, divided into different physicochemical
groups. Aspartate and glutamate belong to the negatively-charged amino acids.
Histidine, arginine and lysine belong to the positively-charged amino acids.
Aspargine, glutamine, serine, threonine, cysteine and tyrosine belong to the polar
amino acids. Glycine, alanine, valine, leucine, isoleucine, methionine, proline,
phenylalanine and tryptophane belong to the non polar amino acids. Aromatic
side groups are to be found among the amino acids histidine, phenylalanine,
tyrosine and tryptophane. In general, it is preferred to substitute an amino acid by
a different amino acid pertaining to another physicochemical group.
According to a preferred embodiment of the invention, the transport protein is a
Botulinus neurotoxin serotype A to G.
In a preferred embodiment of the invention, the transport protein is derived from
the protein sequence of the clostridium botulinum neurotoxin type A (database no.
AAA23262 and CAA51824).
A further embodiment of the present invention relates to a transport protein,
wherein at least one amino acid in the positions 1117, 1202 to 1204, 1252 to
1254, 1262 to 1267, 1270 and 1278 to 1279 of the protein sequence of the
Clostridium botulinum neurotoxin type A (database no. AAA23262 and
CAA51824) has either been removed or been substituted by a naturally occurring
or not naturally occurring amino acid.
A further embodiment of the present invention relates to a transport protein,
wherein amino acids in positions 1092 to 1296 of the protein sequence of the
Clostridium botulinum neurotoxin type A (database no. AAA23262 and
CAA51824) - a region including the ganglioside-binding domain - have been
substituted by the sequence of
Clostridium botulinum neurotoxin type B protein (database no. AAA23211),
amino acids 1079 to 1291,
Clostridium botulinum neurotoxin type Ci protein (database no. CAA51313),
amino acids 1093 to 1291
Clostridium hotulinum neurotoxin type D protein (database no. CAA38175),
amino acids 1080 to 1276,
Clostridium hotulinum neurotoxin type E protein (database no. CAA44558),
amino acids 1067 to 1252,
Clostridium butyricum neurotoxin type E protein (database no. CAA43998),
amino acids 1067 to 1251,
Clostridium botulinum neurotoxin type F protein (database no. CAA57358),
amino acids 1085 to 1278,
Clostridium baratii neurotoxin type F protein (database no. CAA48329),
amino acids 1076 to 1268,
Clostridium botulinum neurotoxin type G protein (database no. CAA52275),
amino acids 1087 to 1297.
Further Hcc-domains suitable for interchangeability with amino acid positions are
apparent from Table 1.
A further embodiment of the present invention relates to a composition containing
a transport protein according to the invention and at least one intervening
molecule (X). The intervening molecule may be a small organic molecule, a
peptide or a protein; preferably covalently bonded, e.g. by a peptide-, ester-,
ether-, sulphide-, disulphide- or carbon-carbon-bond.
In addition, the intervening molecule includes all known therapeutically active
substances, Cytostatics, antibiotics, virustatics, but also immunoglobulins are
preferred in this context.
In order to better utilise the increased affinity of the Trapo, it was bound aminoterminally
to an LC of BoNT/A, B, C-i, D, E, F or G via an amino acid sequence,
which is specifically recognised and split by the protease thrombin, bringing
about a specific TrapoX. The active strength of the said TrapoX, in comparison
with native BoNT/A, was increased and particularly preferred by a factor of at
least 3. This new product, which is free of foreign proteins, will dramatically
reduce the stimulation of the immune systems due to the greater purity of the
material and the low dosage.
A further embodiment of the present invention relates to a transport protein,
wherein the protein is a protease, splitting one or a plurality of proteins of the
release apparatus of neurotransmitters, the protease being selected from the
group of neurotoxins consisting of the LC of the Clostridium botulinum
neurotoxins, in particular of type A, B, d, D, E, F and G or a proteolytically active
fragment of the LC of a Clostridium botulinum neurotoxin, in particular a
neurotoxin of serotype A, B, CL D, E, F and G, the fragment exhibiting at least
0,01% of the proteolytic activity of the native protease, preferably at least 5%,
particularly preferably at least 50%, in particular at least 90%. Preferably, the
transport protein and the protease are derived from the same C. botulinum
neurotoxin serotype, in particular and preferably the HN-domain of the transport
protein and the protease are derived from the C. botulinum neurotoxin serotype A.
The sequences of the proteases are generally accessible at databases and the
database numbers are apparent from Table 1. The proteolytic activity of the
proteases is determined by way of substrate separation kinetics (see Bina et al.
(2002), Biochemistry 41 (6), 1717-23).
The LCs are characterised in that they contain the sequence His-Glu-Leu-Xaa-
His-(Xaa)33.36-Glu(Xaa)84.9o-Glu-(Xaa)ii-Arg-Xaa-Xaa-Tyr, wherein Xaa may be
any amino acid. The transport protein of the present invention is characterised in
that the protein and the protease stem from the previous groups of proteins
and/or proteases.
According to a further embodiment of the present invention, a method for
producing the transport protein is provided. In this case, in a first step a nucleic
acid coding for the transport protein is provided. The coding nucleic acid may
represent in this context RNA, DMA or mixtures thereof. The nucleic acid may
furthermore be modified with regard to its nuclease resistance, such as e.g. by
inserting phosphorthioate bonds. The nucleic acid may be produced from a
starting nucleic acid, the latter being accessible e.g. by cloning from genomic or
cDNA-databases. Moreover, the nucleic acid may be produced directly by solid
phase synthesis. Suitable methods are known to the person skilled in the art. If
one assumes a starting nucleic acid, a specific modification, e.g. by localityspecific
mutagenesis, may be brought about, resulting in at least one addition,
insertion, deletion and/or substitution on the amino acid level. The nucleic acid is
then linked operatively to a suitable promoter. Suitable promoters for expression
in known expression systems are known to the person skilled in the art. The
choice of promoter depends in this case on the expression systems used for
expression, in general, constitutive promoters are preferred, but inducible
promoters may likewise be used. The construct produced in this manner includes
at least one part of a vector, in particular regulatory elements, the vector, for
example, being selected from X-derivates, adenoviruses, baculoviruses, vaccinia
viruses, SV40-viruses and retroviruses. The vector is preferably capable of
expressing the nucleic acid in a given host ceil.
The invention further provides host cells, which contain the vector and are
suitable for expressing the vector. Numerous prokaryotic and eukaryotic
expression systems are known in the state of the art, the host cells being
selected, for example, from prokaryotic cells such as E. coli or B. megaterium,
from eukaryotic cells such as S. cerevisiae and P. pastoris. Although higher
eukaryotic cells, such as insect cells or mammal cells, may be used as well, host
cells are nevertheless preferred, which, like C. botulinum, do not possess a
glycosylation apparatus.
According to a preferred embodiment the nucleic acid codes for the Hcc-domain
of the C. botulinum neurotoxin. This nucleic acid contains endonucleaseinterfaces,
flanking the nucleic acid coding for the He-fragment, the
endonuclease sites being compatible with those of other He-fragments of C.
Doiunnum neurotoxins, in order to permit their easy modular substitution in the
gene coding for the transport protein, while the similarity of the amino acid
sequence is preserved.
If a composition according to the invention is provided, which, apart from the
transport system, further contains at least one intervening molecule, and this
intervening molecule, a peptide or protein, is functionalised either with a carboxyterminal
cysteine or a mercapto-group, then, in an analogous manner, as
described before, the peptide and/or protein may be produced recombinantly, for
example by using binary vectors or various host cells. If the same host cell is
used for the expression both of the transport protein and the peptide or protein,
an intermolecular disulphide bond is preferably formed in situ. For a more
efficient production in the same host cell, the nucleic acid coding for the peptide
or protein may also be translated with that of the transport protein in the same
reading frame, so that a single-chain polypeptide is produced. In this case
preferably an intramolecular disulphide bond is formed in situ. For simple
hydrolysis of the likewise present peptide cross-linking between the transport
protein and the peptide and/or protein, an amino acid sequence is inserted at the
amino-terminus of the transport protein, which is either specifically recognised
and separated by the protease thrombin or by a specific endoprotease of the
host cell.
If this is not possible, an appropriate intermolecular disulphide-linkage, after
separate purification of the transport protein and the protein, may subsequently
be brought about by oxidation processes known to the person skilled in the art.
The peptide or protein may also be obtained directly by synthesis or fragment
condensation. Appropriate methods are known to the person skilled in the art.
The transport protein and the peptide, or protein respectively, are subsequently
purified. For this purpose methods are used, known to the person skilled in the
art, such as e.g. chromatography-methods or electrophoresis,
A further embodiment of the present invention relates to the pharmaceutical
composition, including the transport protein and optionally a pharmaceutically
acceptable excipient, a diluent and/or an additive and which is suitable to treat
the following disorders or diseases: hemt-facial spasms, spasmodic torticollis,
spasticities, dystonias, migraine, pain, disorders of the neck and lumbar vertebral
column, strabism, hypersalivation and depressive diseases.
The pharmaceutical composition is suitable for oral, intravenous, subcutaneous,
intramuscular and topical administration. Intramuscular administration is
preferred. A dosing unit of the pharmaceutical composition contains
approximately 0,1 pg to 1 mg of transport protein and/or the composition
according to the invention.
A further embodiment of the present invention includes a cosmetic composition,
consisting of the transport protein and a pharmaceutical excipient, a diluent
and/or an additive, suitable for treating hyperhydrosis and very pronounced facial
wrinkles. The cosmetic composition is suitable for oral, intravenous,
subcutaneous, intramuscular and topical administration. Intramuscular
administration is preferred. A dosing unit of the cosmetic composition contains
about 0,1 pg to 1 mg of transport protein and/or the composition according to the
invention. The cosmetic composition is suitable to treat hyperhydrosis and facial
wrinkles.
The transport protein described in the present invention may be produced by a
suitable host cell, such as e.g. Escherichia coli, Saccharomyces cerevisiae,
Pichia pastohs or Bacillus megaterium, which multiplies a recombinant
expression vector, the vector coding for a transport protein.
The present invention is elucidated by the accompanying drawings, wherein:
Figure 1 shows that the affinity of the mutated He-fragment of BoNT/A to
synaptosome membrane preparations from the rat brain is three times higher
than that of the H c-fragment of the wild type of BoNT/A.
Figure 2 shows t he binding of different BoNT/A He-fragment mutants to rat brain
synaptosomes, the affinity of the BoNT/A He-fragment wild type being set to
100% as a standard. The first column shows the affinities of the BoNT/A mutants,
showing mutations of the amino acids Y1117 resulting in an increase. The
second column shows further BoNT/A-mutants. The third column shows the
affinities of BoN T/A-mutants exhibiting double mutations, which enhance the
binding to nerve cell membranes (synaptosomes).
Figure 3 shows t he increased neurotoxicity of the Y1117A-mutant of BoNT/A in
comparison with the BoNT/A-wild type on the isolated nervus phrenicus -
diaphragm musd e-preparation of the mouse.
Figure 4 shows the binding of the four BoNT/A He-fragment hybrids HcAB, HcAC,
HcAE and McAT (T = tetanus neurotoxin} in nerve cell membranes
(synaptosomes), the BoNT/A He-fragment wild type being set to 100% as a
standard.
Figure 5 shows the increased neurotoxicity of the total toxin hybrids consisting of
BoNT/A and either the He-fragment or the Hcc-domain of BoNT/B in comparison
with the BoNT/A-wiid type in the isolated nervus phrenicus - diaphragm musclepreparation
of the mouse.
In detail, the present invention contains a transport protein (Trapo), formed by a
modification of the HC of the neurotoxin produced by Clostridium botulinum,
preferably specifically binding to neurons, accommodated intracellulariy by
receptor-mediated endocytosis and translocated from the acid endosomal
compartment into the cytosol of neurons. This protein is used as a transporting
means in order to introduce into the cells proteases and other substances bound
to the said transporting means, unable to penetrate physiologically into the
plasma membrane and to reach the cytosol of nerve cells. The substrates of the
proteases are intracellularly localised proteins and peptides participating in the
transmitter release. After separation of the substrates, the specific functions of
the neurons are blocked; the cells themselves are not damaged. One of these
functions is exocytosis, bringing about the neurotransmitter release. If the release
of transmitters is inhibited, the transmission of signals from cell to cell is blocked.
For example, striated muscles are paralysed if the release of acetyl choline is
inhibited at the neuromuscular contact point. This effect may be used
therapeutically, if TrapoX is applied to nerve ends of spastic or dystonic muscles.
Other active substances are, for example, substances exhibiting anti-viral action-
Conjugated with Trapo, they might be of use for treating viral infections of the
nervous system. The present invention also relates to the use of a transport
protein for inhibiting the release of neurotransmitters.
If patients are treated with the native forms of BoNT/A and B, injection of these
non-human proteins, despite the low dosage, causes the formation of antibodies,
so that the therapy must be stopped in order to prevent anaphylactic shock. By
applying a substance with the same active mechanism having a higher transport
efficiency of the enzymatic activity, the dosage may be lowered drastically and
the formation of antibodies will not occur. These properties are attributed to the
transport protein described herein.
Although examples are stated for application, the suitable mode of application
and the dosage is, in general, individually determined by the treating physician.
Such decisions are routinely made by each physician well versed in the relevant
special field. Thus, the mode of application and the dosage of the neurotoxin may
e.g. be selected in accordance with the invention described herein, based on
criteria such as the solubility of the selected neurotoxin or the intensity of the pain
to be treated.
The treatment interval for native BoNT/A and B is currently three to four months
on average. Prolonging this interval would reduce the risk of the formation of
antibodies and allow a longer treatment period with BoNT. The increase of LC in
the cytosol would extend its decomposition timewise and would thus also prolong
the duration of action. The transport protein described here exhibits a higher
affinity and reception rate than the native HC-A.
The following example merely serves for elucidation and should not be
contemplated in a limiting manner.
Examples
Recombinant expression of the genetically engineered TrapoX in E. coli
The DMA-sequence of the HC of BoNT/A was amplified in chromosomal DNA of
Clostridium botulinum (database no. AAA23262) by means of PCR. For this
purpose, by means of specific oMgonucleotides, the codon for the ami no acid
tyrosine 1117 was substituted by a base triplet coding for the amino acid residue
of alanine. Furthermore, the 5'-end of the gene was supplemented by a DNAsequence,
coding for the amino acids of the recognition sequence of thrombin.
This DMA-sequence was inserted into a bacterial expression vector. The inserted
gene for Trapo was in this case fused with an oligonucleotide at the 3'-end,
coding for a carboxy-terminal affinity peptide such as e.g. the Strep-day, 6xHNday
or Hise-day. The expression vector pAR-Trapo produced in this manner is
the starting basis for adding carrier molecules, such as the LC of the BoNT.
The DNA-sequence of the LC of BoNT/A was amplified by the PCR-method in
the chromosomal DNA-sequence of Clostridium botulinum (database no.
AAA23262) and inserted into the expression vector pAR-Trapo upstream of the
coded thrombin recognition sequence. The expression vector pAR-TrapoX thus
produced was transformed into an E.coli K12 strain and the expression of the
protein TrapoX was induced under the conditions of Biological Safety Level 2 and
in compliance with the rules and regulations issued for the project by the district
government of Hanover, file reference 501g.40654/3/57/3. The over-expressed
TrapoX was isolated in an affinity-chromatographic manner, following the
directions of the manufacturer, as a single-chain protein with a molecular weight
of 150 kDa. The protein was subsequently hydrolysed with thrombin conjugated
on sepharose bringing about a pure protein, the two chains of which remained
interlinked by a disulphide-bridge.
Compared with the wild type of BoNT/A, this protein exhibited an affinity,
increased by 300%, to isolated ganglioside GT1b immobilised on micro titre
plates and to synaptosome membrane preparations from rat brain (Figure 1). The
catalytic activity of the LC-A was not changed, as has been shown in the in vitro
splitting of recombinant SNAP-25. The potency of the TrapoX with regard to
inhibition of the neurotransmitter release in functional synaptosomes from rat
brain had increased by 300%, compared with the native BoNT/A recovered from
Clostridium batulinum. In nerve muscle-preparations of the mouse (HDA), the
potency of the TrapoX was likewise increased by 300% compared with the native
BoNT/A (Figure 2).
Measurement of the binding to rat brain svnaptosomes and the
neurotoxicitv in the HDA of different BoNT/A-mutants
The binding of radioactively marked He-fragments to rat synaptosomes was
measured as stated in Rummel et al., J. Mol. Biol. 326 (2003), 835-847. The
neurotoxictty of the BoNT/A-mutants was determined as described by
Habermann et al., Naunyn Schmiedeberg's Arch. Pharmacol. 311 (1980), 33-40.
The comparison of the binding of different BoNT/A-mutants as compared with the
wild type is shown in the following table:
(Table Removed)
The mutation of individual determined amino acids within the ganglioside binding
pocket of BoNT/A resulted in an increase of the binding to nerve cells. Preferably,
in position 1117, tyrosine is substituted by alanine, cysteine or valine. In
particular, the substitution of the tyrosine residue in position 1117 by alanine
results in an increase of the affinity to about 330%.
Further mutations of individual amino acids from the ganglioside binding pocket
in position 1252 and 1253 result likewise in an increase of the binding. In
particular, the mutation of F1252 in tyrosine and H1253 in lysine resulted in an
increase of the affinity by 110%, and 50% respectively.
Furthermore, increases of the binding to nerve cells can be expected in
mutations in positions 1202,1262, 1270, 1278 and 1279.
Moreover, mutants of BoNT/A were also tested with double mutations, in which
case, in particular, the mutants Y1117C/H1253K and Y1117V/H1253K resulted in
an increase of the binding to synaptosomes (cf. Figure 2).
It was furthermore determined that the increase of the binding, particularly of the
mutant Y1117A of BoNT/A resulted in an increase of the neurotoxicity in the
N.phrenicus - neurotoxicity assay (HDA-Assay) (Figure 3).
Determination of binding and neurotoxicity of BoNT/A Hy.-hybrids
The determination of the binding and the neurotoxicity was performed as
described above.
The results are reflected in the following table and further in Figures 4 and 5.
(Table Removed)
Substitution of the Hcc-domain of BoNT/A by the other serotypes, in particular C.
botulinum neurotoxin B and C. botulinum neurotoxin C, resulted in an increase
of the binding to nerve cells. It was furthermore observed that the substitution of
the Hcc-domain of He-fragment of BoNT/A by the corresponding domain of
tetanus neurotoxin likewise resulted in an increase of the affinity in nerve cells.
The affinity changes also apply to the substitution of the Hcc-domain in the entire
BoNT/A. Figure 5 shows in this context that in a hybrid scAtAAB the increase of
affinity has a similar effect on increased neurotoxicity. If, instead of the Hccdomain,
the entire He-fragment scAtAAB is substituted, corresponding results are
observed. In particular, it was observed that an improvement of the neurotoxicity
by about 350% was noted when substituting the Hcc-domain or the He-fragment
of BoNT/A by that of BoNT/B.
Determination of binding of the BoNT-mutants to the ganglioside GT1b
Ganglioside GT1b [NAcNeua3Gal(J3NAcGal
fi4(NAcNeua8NAcNeua3)GalB4Glc(X] (Sigma-Aldrich) is dissolved in methanol
and applied to high-affinity 96-cup polystyrene-micro titre plates (Corning; 1ug
GT1b in 100 ul/cup) or, in the case of competition assays to high-affinity CS
single fracture strip plates with 125l-BoNTs (Greiner Bio-ohne; 0,1 ug GT1b in 100
Ml / cup). The solvent is evaporated at room temperature and the cups are rinsed
three times with a binding buffer (10mM Tris-HCI, 10mM Na2HPO4, 0,5% BSA,
pH7,2). The non specific binding sites are then blocked by incubation for two
hours in PBS/Tween [140 mM NaCI, 7 mM KCI, 10 mM Na2HPO4, 1,8 mM
KH2PO4, 0,05% (VA/) Tween 20, pH 7,2], supplemented by 3% (w/v) BSA. The
binding assays are carried out in binding buffers (100ul / cup) for 2 hours at room
temperature either with increasing quantities of the wild type or specific quantities
of the mutants. Unbound protein is removed in 3 rinsing steps, each with 250ul
PBS/Tween buffer. Bound He-fragments are identified by incubation with Strep
Tactin conjugated with alkaline phosphatase (ST-AP, IBA GmbH) in a binding
buffer for a duration of 2 hours at room temperature according to manufacturer's
instructions, p-nitrophenyl phosphate (1 mg/ml in 100 mM glycine, 1 mM MgCb, 1
mM ZnCb, pH 10,4), which ultimately serves as substrate for the alkaline
phosphatase. The desphorphoryiation reaction is stopped by adding a 3 M NaOH
solution and the extinction is measured at 405 nm using a Spectra Count micro
plate reading device (Packard). The competition assays are performed over a
period of 2 hours at room temperature in a 100ul binding buffer with
700000cpm/cup [1251]-BoNT, different quantities of native BoNT or recombinant
He-fragment. After incubation and removal of the supernatants the cups are
rinsed three times with PBS/Tween buffer, dried and separated. The quantities of
bound radioactiveiy marked BoNT are then determined in an automatic y-counter
(Wallac 1480 Wizard 3).

^ WE CLAIM:
1. A transport protein, obtainable by modification of the heavy chain of the
neurotoxin fornied by Clostridium botulinum, wherein the neurotoxin is
botulinum neurotoxin type A (BoNT/A), and wherein the transport protein binds
to nerve cells with a higher affinity than the native neurotoxin, wherein at least
one amino acid in the positions 1117, 1252, 1253, 1270, and 1278 to 1279 of
the botulinum neurotoxin type A protein sequence is removed or substituted,
either by an amino acid which is naturally occurring or by an amino acid which
is of non-natural origin.
•
2. A transport protein, obtainable by modification of the heavy chain of the
neurotoxin formed by Clostridium botulinum, wherein the neurotoxin is
botulinum neurotoxin type A (BoNT/A), wherein the transport protein binds to
nerve cells with higher affinity than the native neurotoxin, and wherein the
amino acids 1092 to 1296 of Clostridium botulinum neurotoxm type A, which
contain the ganglioside-binding domain, are substituted with one of the
following sequences:
(a) Clostridium botulinum neurotoxin type B protein, amino acids 1079 to
1291, or
(b) Clostridium botulinum neurotoxin type Ci protein, amino acids 1093 to
1291, or
(c) Hcc-domain of Clostridium tetani neurotoxin proteins.
3. A transport protein, obtainable by modification of the heavy chain of the
neurotoxin formed by Clostridium botulinum, wherein the neurotoxin is
botulinum neurotoxin type A (BoNT/A), wherein the transport protein binds to
nerve cells with higher affinity than the native neurotoxin, and wherein the
complete He-fragment consisting of amino acids 867 to 1296 of BoNT/A is
substituted by the He-fragment consisting of amino acids 866 to 1291 of
BoNT/B.
4. The transport protein as claimed in any one of claims 1 to 3, wherein the
protein binds specifically to nerve cells and enters the cells by endocytosis.
27
1
^ 5. The transport protein as claimed in any one of claims 1 to 3, wherein the
protein binds specifically to complex gangliosides of cholinergic motor
neurons, localised in the plasma membrane, preferably GT1b.
6. The transport protein as claimed in any one of claims 1 to 5, wherein the
protein exhibits an affinity that is at least 15% higher than the native
neurotoxin, preferably at least 50% higher, particularly preferably at least 80%
higher, in particular at least 90% higher.
7. The transport protein as claimed in claim 1, wherein the amino acid in position
1117 of the botulinum neurotoxin type A protein sequences has been
removed or substituted by an amino acid, either occurring naturally or of non
natural origin.
8. The transport protein as claimed in claim 7, wherein the substituted amino
acid is either alanine, cysteine, glutamate, phenyl alanine, isoleucine, leucine,
methionine, asparagine, proline, glutamine, serine, threonine or valine.
9. The transport protein as claimed in claim 8, wherein the substituted amino
acid is alanine, cysteine or valine.
10. The transport protein as claimed in claim 1, wherein the amino acid of the
botulinum neurotoxin type A protein sequences in position 1252 of the
botulinum neurotoxin type A protein sequences is substituted by tyrosine or in
position 1253 by lysine.
11. The transport protein as claimed in claim 1, wherein two or three amino acids
selected from the positions 1117, 1252, 1253, 1270 and 1278 to 1279 of the
botulinum neurotoxin type A protein sequences are removed or substituted.
12. The transport protein as claimed in claim 11, wherein two or three amino
acids at positions 1117/1252, 1117/1253, 1117/1262, 1117/1278, 1117/1279
or 1117/1252/1253 are removed or substituted.
13. The transport protein as claimed in claim 11 or 12, wherein said two or three
substituted amino acids are selected from the group consisting of
28
•>• Y1117A/F1252Y, Y1117A/H1253K, Y1117AA/1262I, Y1117A/L1278H,
Y1117A/G1279N, Y1117C/F1252Y, Y1117C/H1253K, Y1117CA/1262I,
Y1117C/L1278H, Y1117C/G1279N, Y1117V/F1252Y, Y1117V/H1253K,
Y1117VA/1262I, Y1117V/L1278H, Y1117V/G1279N,
Y1117A/F1252Y/H1253K, Y1117C/F1252Y/H1253K and
Y1117V/F1252/H1253K.
14. A transport protein as claimed in any one of claims 1 to 13 covalently bonded
to at least one intervening molecule, wherein the intervening molecule is a
small organic molecule, a peptide, or a protein.
15. The transport protein as claimed in claim 14, wherein the intervening molecule
is covalently bonded to the transport protein by a peptide-bond, ester-bond,
ether-bond sulphide-bond, disulphide-bond or a carbon-carbon bond.
16. The transport protein as claimed in claim 14, wherein the small organic
molecule is a virustatic, a cytostatic, an antibiotic or an immunoglobulin.
17. The transport protein as claimed in claim 14, wherein the intervening molecule
is a protein that comprises a protease.
18. The transport protein as claimed in claim 17, wherein the protease comprises
a neurotoxin protein of Clostridium botulinum type A, B, Ci, D, E, F or G.
19. The transport protein as claimed in claim 18, characterised in that said
transport protein contains the secljuence His-Glu-Leu-Xaa-His-(Xaa)33.35-Glu-
(Xaa)84-9o-Glu-(Xaa)ii-Arg-Xaa-Xaa-Tyr, wherein Xaa may be any amino acid.
20. The transport protein as claimed in claim 17, wherein the protease contains a
proteolytically active fragment of the neurotoxin of Clostridium botulinum type
A, B,Ci,D, E,ForG.
21. The transport protein as claimed in claim 20, characterised in that said
transport protein contains the sequence His-Glu-Leu-Xaa-His-(Xaa)33.35-Glu-
(Xaa)84-9o-Glu-(Xaa)ii-Arg-Xaa-Xaa-Tyr, wherein Xaa may be any amino acid.
29
9 22. The transport protein as claimed in any one of claims 17 to 21, wherein the
protease and the transport protein are covalently bonded by an amino acid
sequence, which is specifically recognised and cleaved by an endopeptidase.
23. The transport protein as claimed in claim 22, wherein after cleavage by the
endopeptidase a disulphide-bridge interlinks the protease and the transport
protein, which, in turn, results in the formation of an active holotoxin.
24. A host cell containing a recombinant expression vector, the expression vector
coding for a transport protein as claimed in any one of claims 1 to 23, wherein
the host cell is a cell of Escherichia coli, Saccharomyces cerevisiae, Pichia
pastoris or Bacillus megaterium.
25. An expression vector, wherein the vector includes a nucleic acid, coding for a
transport protein as claimed in any one of claims 1 to 23. Dated this 25**^ day of March 2007 \^^:
J r a r i KUMARI
OF K & SFARTNERS
AGENT FOR THE APPLICANT(S) ;
i
30

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Patent Number

268321

Indian Patent Application Number

2152/DELNP/2007

PG Journal Number

35/2015

Publication Date

28-Aug-2015

Grant Date

26-Aug-2015

Date of Filing

20-Mar-2007

Name of Patentee

SYNTAXIN LIMITED

Applicant Address

FRIEDRICH-EBERT-STR.27, 30851 LANGENHAGEN, GERMANY

Inventors:

#	Inventor's Name	Inventor's Address
1	RUMMEL, ANDREAS	HAUPTSTR. 192, 30826 GARBSEN, GERMANY

PCT International Classification Number

C07K 14/33

PCT International Application Number

PCT/EP2005/009554

PCT International Filing date

2005-09-06

PCT Conventions:

#	PCT Application Number	Date of Convention	Priority Country
1	10 2004 043 009.8	2004-09-06	Germany