Title of Invention	"RECOMBINANT BACTERIA FOR PRODUCING DEOXYVIOLACEIN AND USES THEREOF"
Abstract	Recombinant bacteria for producing deoxyviolacein and uses thereof are provided, wherein the recombinant bacteria is obtained by introducing the deoxyviolacein synthesis-related gene cluster into Escherichia coli BL21-CodonPlus (DE3)-RIL or Pseudomonas putida mt-2. The deoxyviolacein synthesis-related gene cluster is obtained by knocking out VioD gene from the violacein synthesis-related gene cluster composed of VioA, VioB, VioC, VioD and VioE, and the nucleotide sequence is as shown in the SEQ ID NO: 1 in the sequence listing. A method for producing deoxyviolacein by fermenting the recombinant bacteria to produce deoxyviolacein by using L-tryptophan as substrate is provided. The method has high efficiency of deoxyviolacein production, the deoxyviolacein produced is convenient to be extracted, and simple to be separated and purified.

Title of Invention

"RECOMBINANT BACTERIA FOR PRODUCING DEOXYVIOLACEIN AND USES THEREOF"

Abstract

Recombinant bacteria for producing deoxyviolacein and uses thereof are provided, wherein the recombinant bacteria is obtained by introducing the deoxyviolacein synthesis-related gene cluster into Escherichia coli BL21-CodonPlus (DE3)-RIL or Pseudomonas putida mt-2. The deoxyviolacein synthesis-related gene cluster is obtained by knocking out VioD gene from the violacein synthesis-related gene cluster composed of VioA, VioB, VioC, VioD and VioE, and the nucleotide sequence is as shown in the SEQ ID NO: 1 in the sequence listing. A method for producing deoxyviolacein by fermenting the recombinant bacteria to produce deoxyviolacein by using L-tryptophan as substrate is provided. The method has high efficiency of deoxyviolacein production, the deoxyviolacein produced is convenient to be extracted, and simple to be separated and purified.

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Patent Number

270257

Indian Patent Application Number

940/DELNP/2011

PG Journal Number

50/2015

Publication Date

11-Dec-2015

Grant Date

07-Dec-2015

Date of Filing

08-Feb-2011

Name of Patentee

TSINGHUA UNIVERSITY

Applicant Address

ROOM 450#, GONGWU BUILDING, INSTITUTE OF BIOCHEMICAL ENGINEERING DEPARTMENT OF CHEMICAL ENGINEERING TSINGHUA UNIVERSITY, HAIDIAN DISTRICT, BEIJING 100084, CHINA

Inventors:

#	Inventor's Name	Inventor's Address
1	XINHUI XING	ROOM 450#, GONGWU BUILDING, INSTITUTE OF BIOCHEMICAL ENGINEERING DEPARTMENT OF CHEMICAL ENGINEERING TSINGHUA UNIVERSITY, HAIDIAN DISTRICT, BEIJING 100084, CHINA
2	PEIXIA JIANG	ROOM 450#, GONGWU BUILDING, INSTITUTE OF BIOCHEMICAL ENGINEERING DEPARTMENT OF CHEMICAL ENGINEERING TSINGHUA UNIVERSITY, HAIDIAN DISTRICT, BEIJING 100084, CHINA

PCT International Classification Number

C12P 17/16

PCT International Application Number

PCT/CN2009/000430

PCT International Filing date

2009-04-22

PCT Conventions:

#	PCT Application Number	Date of Convention	Priority Country
1	20081011661.3	2008-07-11	China
2	200810224359.1	2008-10-17	China