Title of Invention |
PURIFICATION OF CARRIER PROTEIN- ANTIGEN COMPLEXES |
Abstract |
A method of isolating and purifying protein polysaccharide conjugates using hydrophobic interaction chromatography. Crude protein polysaccharide conjugate mixtures are subjected to ultrafiltration to remove unreacted protein, and loaded onto a column filled with a suitable hydrophobic resin. Elution is effected with salt solutions of decreasing ionic strength. Protein polysaccharide conjugates substantially free of unconjugated polysaccharide are provided. |
Documents:
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