| Abstract |
Process for immobilization of an enzyme, characterized by the steps comprising (a) activating said support with 1-ethyl-3 (3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysulfosuccinimide, (b) covalent coupling of activated support with said enzyme at a pH ranging from about 4.5 to 6.5 and temperature of 4°C, (c) packing of column with enzyme coupled support, (d) passing culture supernatant containing capsular polysaccharide of interest through the column followed by a suitable contact time, and (e) diafiltration with molecular weight cut-off ranging from about 100 kDa to 300 kDa of eluate to remove degraded nucleic acid ,protein and polysaccharide.The covalently immobilized enzymes affords substantial reduction of enzyme as a residual contaminant in the supernatant. The said immobilized enzymes can be recycled for atleast 4 times. |