| Title of Invention | "TRICYCLIC NITROGEN CONTAINING COMPOUNDS AS ANTIBACTERIAL AGENTS" |
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| Abstract | Tricyclic nitrogen containing compounds and their use as antibacterials. Z1 and Z2 are independently selected from CH and N. |
| Full Text | TRICYCLIC NITROGEN CONTAINING COMPOUNDS AS ANTIBACTERIAL AGENTS This invention relates to novel compounds, compositions containing them and their use as antibacterials including use in the treatment of tuberculosis. WO02/08224, WO02/50061, WO02/56882, WO02/96907, WO2003087098, WO2003010138, WO2003064421, WO2003064431, WO2004002992, WO2004002490, WO2004014361, WO2004041210,WO2004096982, WO2002050036, WO2004058144, WO2004087145, WO2006002047, WO2006014580, WO2006010040, WO2006017326, WO2006012396, WO2006017468, WO2006020561, WO2006081179, WO2006081264, WO2006081289, WO2006081178, WO2006081182, WO01/25227, WO02/40474, WO02/07572, WO2004024712, WO2004024713, WO2004035569, WO2004087647, WO2004089947, WO2005016916, WO2005097781, WO2006010831, WO2006021448, WO2006032466, WO2006038172, WO2006046552, WO06099884, WO06126171, WO06137485, WO06105289, WO06125974, WO06134378, WO07016610, WO07081597, WO07071936, WO07115947, WO07118130, WO07122258, WO08006648, WO08003690 and WO08009700 disclose quinoline, naphthyridine, morpholine, cyclohexane, piperidine and piperazine derivatives having antibacterial activity. WO2004104000 discloses tricyclic condensed ring compounds capable of selectively acting on cannabinoid receptors. WO2003048081, WO2003048158 and US2003232804 disclose glycinamides as Factor Xa inhibitors. This invention provides a compound of formula (I) or a pharmaceutically acceptable salt and/or N-oxide thereof: A—NR2—UR5 wherein: Z1 and Z2 are independently selected from CH and N; R1a and R1b are independently selected from hydrogen; halogen; cyano; (C1-6)alkyl; (C1-6)alkylthio; trifluoromethyl; trifluoromethoxy; carboxy; hydroxy optionally substituted with (C1-6)alkyl or (C1-6)alkoxy-substituted(C1-6)alkyl; (C1-6)alkoxysubstituted( C1-6)alkyl; hydroxy (C1-6)alkyl; an amino group optionally N-substituted by - 1 - one or two (C1-6)alkyl, formyl, (C1-6)alkylcarbonyl or (C1-6)alkylsulphonyl groups; and aminocarbonyl wherein the amino group is optionally substituted by (C1-4)alkyl; provided that R1a and R1b are H when Z2 or Z1 is N, respectively; R2 is hydrogen, or (C1-4)alkyl, or together with R6 forms Y as defined below; A is a group (i): R3 (ia) or (ib) in which: R3 is as defined for R1a and R1b or is oxo and n is 1 or 2: or A is a group (ii) R7 W3^X^CH2 W1\KW2 W1, W2 and W3 are CR4R8 or W2 and W3 are CR4R8 and W1 represents a bond between W3 and N. XisO,CR4R8,orNR6; one R4 is as defined for R1a and R1b and the remainder and R8 are hydrogen or one R4andR8 are together oxo and the remainder are hydrogen; R6 is hydrogen or (C1-6)alkyl; or together with R2 forms Y; R7 is hydrogen; halogen; hydroxy optionally substituted with (C1-6)alkyl; or (C1- 6)alkyl; Y is CR4R8CH2; CH2CR4R8; (C=O); CR4R8; CR4R8(C=O); or (C=O)CR4R8; or when X is CR4R8, R8 and R7 together represent a bond; U is selected from CO, and CH2 and R5 is an optionally substituted bicyclic carbocyclic or heterocyclic ring system (B): - 2 - ( (a) I (b) ) Y - ^ ^ ^ (B) containing up to four heteroatoms in each ring in which at least one of rings (a)and (b) is aromatic; is C or N when part of an aromatic ring, or CR14 when part of a non-aromatic ring; X2 is N, NR13, O, S(O)x, CO or CR14 when part of an aromatic or non-aromatic ring or may in addition be CR14R15 when part of a non aromatic ring; X3 and X5 are independently N or C; is a 0 to 4 atom linker group each atom of which is independently selected from N, NRI3, O, S(O)X, CO and CR14 when part of an aromatic or non-aromatic ring or may additionally be CRl 4R1 5 when part of a non aromatic ring; Y2 is a 2 to 6 atom linker group, each atom of Y2 being independently selected from N, NR13, O, S(O)X, CO, CRl4 when part of an aromatic or non-aromatic ring or may additionally be CR14R15 when part of a non aromatic ring; each of R14 and Rl5 is independently selected from: H; (C1-4)aIkylthio; halo; carboxy(C1-4)alkyl; (C1-4)alkyl; (C1-4)alkoxycarbonyl; (C1-4)alkylcarbonyl; (C1- 4)alkoxy (C1-4)alkyl; hydroxy; hydroxy(C1-4)alkyl; (C1-4)alkoxy; nitro; cyano; carboxy, amino or aminocarbonyl optionally mono- or di-substituted by (C1-4)alkyl; or R1 4andR1 5 may together represent oxo; each Rl3 is independently H; trifluoromethyl; (C1-4)alkyl optionally substituted by hydroxy, (C1-6)alkoxy, (C1-6)alkylthio, halo or trifluoromethyl; (C2-4)alkenyl; (C1- 4)alkoxycarbonyl; (C1-4)alkylcarbonyl; (C1-6)alkylsulphonyl; aminocarbonyl wherein the amino group is optionally mono or disubstituted by (C1-4)alkyl; and each x is independently 0, 1 or 2. This invention also provides a method of treatment of bacterial infections including tuberculosis in mammals, particularly in man, which method comprises the administration to a mammal in need of such treatment an effective amount of a compound of formula (I), or a pharmaceutically acceptable salt and/or N-oxide thereof. The invention also provides the use of a compound of formula (I), or a pharmaceutically acceptable salt and/or N-oxide thereof, in the manufacture of a medicament for use in the treatment of bacterial infections including tuberculosis in mammals. - 3 - The invention also provides a pharmaceutical composition comprising a compound of formula (I), or a pharmaceutic ally acceptable salt and/or N-oxide thereof, and a pharmaceutically acceptable carrier. In one aspect one of Zl and Z2 is CH or N and the other is CH. In particular aspects: (i) Zl and Z2 are both CH; (ii) ZlisNandZ2isCH; (iii) Zl is CH and Z2 is N. In a particular aspect R1a and R1b are independently hydrogen, (C1-4)alkoxy, (C1-4)alkylthio, (C1-4)alkyl, cyano, carboxy, hydroxymethyl or halogen; more particularly hydrogen, methoxy, methyl, cyano, or halogen. In particular embodiments Rla and R1b are hydrogen. In a particular aspect R2 is hydrogen. Particular examples of R3 include hydrogen; optionally substituted hydroxy; optionally substituted amino; halogen; (C1-4) alkyl; l-hydroxy-(C1-4) alkyl; optionally substituted aminocarbonyl. More particular R3 groups are hydrogen; CONH2; 1- hydroxyalkyl e.g. CH2OH; optionally substituted hydroxy e.g. methoxy; optionally substituted amino; and halogen, in particular fluoro. Most particularly R3 is hydrogen, hydroxy or fluoro. In a particular aspect, when A is (ia), n is 1. In a further aspect R3 is in the 3- or 4-position. In a more particular aspect, A is (ia), n is 1 and R3 is in the 3-position, and more particularly is cis to the NR2 group. In particular embodiments, A is a group (ia) in which n is 1 and R3 is hydrogen or hydroxy. More particularly where A is 3-hydroxypiperidin- 4-yl the configuration is (3R,4S) or (3S,4R). Alternatively and more particularly where A is piperidin-4-yl the configuration is (3R,4S). In an alternative more particular aspect, when A is (ia), n is is in the 4- position and is methyl. In a particular aspect, when A is (ii), X is CR4R8 and R8 is H and R4 is H or OH and more particularly OH is trans to R7. In a further aspect W1 is a bond. In another aspect R7 is H. In an additional aspect W1 is a bond, W2 and W3 are both CH2 and R7 is H. Where A is 4-hydroxypyrrolidin-3-ylmethyl, in a particular aspect the configuration is (3S,4S). Where A is pyrrolidin-3-ylmethyl, in a particular aspect the configuration is 3S. In a particular aspect, when A is (ii), X is O, R7 is H and W1, W2 and W3 are each CH2. In certain embodiments U is CH2. - 4 - In certain embodiments R5 is an aromatic heterocyclic ring (B) having 8-11 ring atoms including 2-4 heteroatoms of which at least one is N or NR13 in which, in particular embodiments, Y2 contains 2-3 heteroatoms, one of which is S and 1-2 are N, with one N bonded to X3. In alternative embodiments the heterocyclic ring (B) has ring (a) aromatic selected from optionally substituted benzo, pyrido, pyridazino and pyrimidino and ring (b) non aromatic and Y2 has 3-5 atoms, more particularly 4 atoms, including at least one heteroatom, with O, S, CH2 or NR13 bonded to X5 where R13 is other than hydrogen, and either NHCO bonded via N to X3, or O, S, CH2 or NH bonded to X3. In a particular aspect the ring (a) contains aromatic nitrogen, and more particularly ring (a) is pyridine or pyrazine. Examples of rings (B) include optionally substituted: (a) and (b) aromatic lH-pyrrolo[2,3-b]-pyridin-2-yl, lH-pyrrolo[3,2-b]-pyridin-2-yl, 3H-imidazo[4,5-b]- pyrid-2-yl, 3H-quinazolin-4-one-2-yl, benzimidazol-2-yl, benzo[l,2,3]-thiadiazol-5-yl, benzo[l,2,5]-oxadiazol-5-yl, benzofur-2-yl, benzothiazol-2-yl, benzo[b]thiophen-2-yl, benzoxazol-2-yl, chromen-4-one-3-yl, imidazo[l,2-a]pyridin-2-yl, imidazo-[l,2-a]- pyrimidin-2-yl, indol-2-yl, indol-6-yl, isoquinolin-3-yl, [l,8]-naphthyridine-3-yl, oxazolo[4,5-b]-pyridin-2-yl, quinolin-2-yi, quinolin-3-yl, quinoxalin-2-yl, naphthalen-2- yl, l,3-dioxo-isoindol-2yl, lH-benzotriazol-5-yl, lH-indol-5-yl, 3H-benzooxazol-2-one- 6-yl, 3H-benzooxazol-2-thione-6-yl, 3H-benzothiazol-2-one-5-yl, 3H-quinazolin-4-one- 6-yl,pyrido[l,2-a]pyrimidin-4-one-3-yl (4-oxo-4H-pyrido[l,2-a]pyrimidin-3-yl), benzo[ 1,2,3]thiadiazol-6-yl, benzo[ 1,2,5]thiadiazol-5-yl, benzo[l ,4]oxazin-2-one-3-yl, benzothiazol-5-yl, benzothiazol-6-yl, cinnolin-3-yl, imidazo[l,2-b]pyridazin-2-yl, pyrazolo[ 1,5-a]pyrazin-2-yl, pyrazolo[ 1,5-a]pyridin-2-yl, pyrazolo[l ,5-a]pyrimidin-6-yl, pyrazolo [5,1 -c] [ 1,2,4]triazin-3 -yl, pyrido [ 1,2-a]pyrimdin-4-one-2-yl (4-oxo-4Hpyrido[ l,2-a]pyrimidin-2-yl), quinazolin-2-yl, quinoxalin-6-yl, thiazolo[3,2-a]pyrimidin- 5-one-7-yl, thiazolo[5,4-b]pyridin-2-yl, thieno[3,2-b]pyridin-6-yl, thiazolo[5,4-b]pyridin- 6-yl, thiazolo[4,5-b]pyridin-5-yl, [l,2,3]thiadiazolo[5,4-b]pyridin-6-yl, 2H-isoquinolin- 1 -one-3-yl (1 -oxo-1,2-dihydro-isoquinolin-3-yl), [ 1,2,3]thiadiazolo[5,4-6]pyridine-6-yl - 5 - -co -co -co xo H H H . H XX> TO xx> xx> XX) is the point of attachment (a) is non aromatic (2S)-2,3-dihydro-lH-indol-2-yl,(2S)-2,3-dihydro-benzo[l,4]dioxine-2-yl,3-(R,S)-3,4- dihydro-2H-benzo[l,4]thiazin-3-yl, 3-(R)-2,3-dihydro-[l,4]dioxino[2,3-b]pyridin-3-yl, 3- 6- (S)-2)3-dihydro-[l,4]dioxino[2,3-b]pyridin-3-yl, 2,3-dihydro-benzo[l,4]dioxan-2-yl, 3- substituted-3H-quinazolin-4-one-2-yl, . ioo xo xo Xx rx) xo o is the point of attachment (b) is non aromatic l,l,3-trioxo-l,2,3,4-tetrahydrol /6_benzo[l,4] thiazin-6-yl, benzo[l,3]dioxol-5-yl, 2,3- dihydro-benzo[1,4]dioxin-6-yl, 3-substituted-3H-benzooxazol-2-one-6-yl, 3-substituted- 3H-benzooxazole-2-thione-6-yl, 3-substituted-3H-benzothiazol-2-one-6-yl, 4Hbenzo[ l,4]oxazin-3-one-6-yl (3-oxo-3,4-dihydro-2H-benzo[l,4]oxazin-6-yl), 4Hbenzo[ l,4]thiazin-3-one-6-yl(3-oxo-3,4-dihydro-2H-benzo[l,4]thiazin-6-yl), 4Hbenzo[ l,4]oxazin-3-one-7-yl, 4-oxo-2,3,4,5-tetrahydro-benzo[b][l,4]thiazepine-7-yl, 5- oxo-2,3-dihydro-5H-thiazolo[3,2-a]pyrimidin-6-yl, lH-pyrido[2,3-b][l,4]thiazin-2-one- 7-yl(2-oxo-2,3-dihydro-lH-pyrido[2,3-b]thiazin-7-yl), 2,3-dihydro-lH-pyrido[2,3- b][l,4]thiazin-7-yl, 2-oxo-2,3-dihydro-lH-pyrido[3,4-b]thiazin-7-yl, 2,3-dihydro- [l,4]dioxino[2,3-b]pyridin-6-yl, 2,3-dihydro-[l,4]dioxino[2,3-c]pyridin-7-yl, 2,3- dihydro-[ 1,4]dioxino[2,3-b]pyridin-7-yl, 3,4-dihydro-2H-benzo[ 1,4]oxazin-6-yl, 3,4- dihydro-2H-benzo[ 1,4]thiazin-6-yl, 3-oxo-3,4-dihydro-2H-pyrido[3,2-b][ 1,4]oxazin-6-yl, 3,4-dihydro-2H-pyrido[3,2-b][l,4]thiazin-6-yl,3-oxo-3,4-dihydro-2H-pyrido[3,2- b][ 1,4]thiazin-6-yl, 3,4-dihydro- lH-quinolin-2-one-7-yl, 3,4-dihydro-1 H-quinoxalin-2- one-7-yl, 6,7-dihydro-4H-pyrazolo[l,5-a]pyrimidin-5-one-2-yl, 5,6,7,8-tetrahydro- [ 1,8]naphthyridin-2-yl (1,2,3,4-tetrahydro-[l ,8]naphthyridin-7-yl), 2-oxo-3,4-dihydrolH-[ l,8]naphthyridin-6-yl, 6-oxo-6,7-dihydro-5H-pyridazino[3,4-b][l,4]thiazin-3-yl (6- oxo-6,7-dihydro-5H-8-thia-l,2,5-triaza-naphthalen-3-yl), 2-oxo-2,3-dihydro-lHpyrido[ 3,4-b][ 1,4]oxazin-7-yl, 2-oxo-2,3-dihydro-1 H-pyrido[2,3-b][ 1,4]oxazin-7-yl, 6,7- dihydro-[l,4]dioxino[2,3-d]pyrimidin-2-yl, [l,3]oxathiolo[5,4-c]pyridin-6-yl, 3,4- dihydro-2H-pyrano[2,3-c]pyridin-6-yl, 2,3-dihydro[ 1,4]oxathiino[2,3-c]pyridin-7-yl, 6,7- dihydro[l,4]dioxino[2,3-c]pyridazin-3-yl, 5,6,7,8-tetrahydroisoquinolin-3-yl, 6,7- dihydro-5H-cyclopenta[c]pyridin-3-yl, l,3-dihydrofuro[3,4-c]pyridin-6-yl, 3,4-dihydro- 2H-[l,4]oxathiepino[2,3-c]pyridin-8-yl, [l,3]oxathiolo[4,5-c]pyridin-6-yl, 6,7- dihydro[l,4]oxathiino[2,3-c]pyridazin-3-yl, 6,7-dihydro-5H-pyrano[2,3-c]pyridazin-3-yl, 5,6-dihydrofuro[2,3-c]pyridazin-3-yl, 2,3-dihydroniro[2,3-c]pyridin-5-yl, 2-substituted - 7 - 1H-pyrimido[5,4-6][l,4]oxazin-7(6H)-one, 2-substituted 5,6-dihydropyrido[2,3- d]pyrimidin-7(lH)-one, 7- substituted 2H-chromen-2-one, 7-substituted 2H-pyrano[2,3- 6]pyridin-2-one, 2-substituted 6,7-dihydro-5H-pyrano[2,3-d]pyrimidine, 8-substitited 2Hpyrido[ l ,2-a]pyrimidin-2-one, 2,3-dihydro-l -benzofuran-5-yl, 1 H-pyrimido[5,4- b][ 1,4]thiazin-7(6H)-one-2-yl, 3,4-dihydro-2H-chromen-7-yl, 2,3-dihydro-1 -benzofuran- 6-yl, 3,4-dihydro-2H-chromen-6-yl, 3,4-dihydro-2H-pyrido[3,2-b][l,4]oxazine-6-yl, 3,4- dihydro-2H-pyrido[3,2-b][l,4]thiazine-6-yl, 6,7-dihydro-5H-thieno[3,2-6]pyran-2-yl, 2,3,4,5 -tetrahydro-1,5 -benzothiazepine-7-yl. - 8 - H H u where R is an optional substituent is the point of attachment In some embodiments R13 is H if in ring (a) or in addition (C1-4)alkyl such as methyl or isopropyl when in ring (b). More particularly, in ring (b) Rl3 is H when NR13 is bonded to X3 and (C1-4)alkyl when NR13 is bonded to X5. In futher embodiments R14 and R15 are independently selected from hydrogen, halo, hydroxy, (C1-4) alkyl, (C1-4)alkoxy, nitro and cyano. More particularly Rl5 is hydrogen. More particularly each R14 is selected from hydrogen, chloro, fluoro, hydroxy, methyl, methoxy, nitro and cyano. Still more particularly R14 is selected from hydrogen, fluorine or nitro. Most particularly R14 and R15 are each H. Particular groups R5 include: [l,2,3]thiadiazolo[5,4-b]pyridin-6-yl 1 H-pyrrolo[2,3-b]pyridin-2-yl 2,3-dihydro-[ 1,4]dioxino[2,3-b]pyridin-6-yl 2,3-dihydro-[l,4]dioxino[2,3-b]pyridin-7-yl 2,3-dihydro-[l,4]dioxino[2,3-c]pyridin-7-yl 2,3-dihydro-benzo[l,4]dioxin-6-yl 2-oxo-2,3-dihydro- lH-pyrido[2,3-b][ 1,4]oxazin-7-yl 2-oxo-2,3-dihydro-lH-pyrido[2,3-b][l,4]thiazin-7-yl 3,4-dihydro-2H-benzo[l,4]oxazin-6-yl 3-methyl-2-oxo-2,3-dihydro-benzooxazol-6-yl 3-oxo-3,4-dihydro-2H-benzo[ 1,4]oxazin-6-yl 3-oxo-3,4-dihydro-2H-pyrido[3,2-b] [ 1,4]oxazin,-6-yl 3-oxo-3,4-dihydro-2H-benzo[ 1,4]thiazin-6-yl (4H-benzo[ 1,4] thiazin-3-one-6-yl) 4-oxo-4H-pyrido [ 1,2-a]pyrimidin-2-yl - 9 - 6-nitro-benzo[l ,3]dioxol-5-yl 7-fluoro-3-oxo-3,4-dihydro-2H-benzo[l,4] oxazin-6-yl 8-hydroxy-l-oxo-l,2-dihydro-isoquinolm-3-yl 8-hydroxyquinolin-2-yl benzo[ 1,2,3]thiadiazol-5-yl benzo[l ,2,5]thiadiazol-5-yl benzothiazol-5-yl thiazolo-[5,4-b]pyridin-6-yl 3-oxo-3,4-dihydro-2H-pyrido[3,2-b][ 1,4Jthiazin-6-yl 7-chloro-3-oxo-3,4-dihydro-2H-pyrido[3,2-b][l,4]thiazin-6-yl 7-chloro-3-oxo-3,4-dihydro-2H-pyrido[3,2-b][l,4]oxazin-6-yl 7-fluoro-3-oxo-3,4-dihydro-2H-pyrido[3,2-b][ 1,4]thiazin-6-yl 2-oxo-2,3-dihydro-1H-pyrido[3,4-b][ 1,4]thiazin-7-yl [1,3]oxathiolo[5,4-c]pyridin-6-yl 3,4-dihydro-2H-pyrano[2,3-c]pyridin-6-yl 5-carbonitro-2,3-dihydro-1,4-benzodioxin-7-yl 2,3-dihydro[l,4]oxathiino[2,3-c]pyridin-7-yl 6,7-dihydro[l,4]dioxino[2,3-c]pyridazin-3-yl 5,6,7,8-tetrahydroisoquinolin-3-yl 6,7-dihydro-5H-cyclopenta[c]pyridin-3-yl 1,3-dihydrofuro[3,4-c]pyridin-6-yl 6-fluoro-2,3-dihydro-1,4-benzodioxin-7-yl 3,4-dihydro-2H-[l,4]oxathiepino[2,3-c]pyridin-8-yl, [l,3]oxathiolo[4,5-c]pyridine-6-yl 2,3-dihydro-1 -benzofuran-5 -yl 6,7-dihydro[l,4]oxathiino[2,3-c]pyridazin-3-yl 6,7-dihydro-5H-pyrano[2,3-c]pyridazin-3-yl 5,6-dihydrofuro[2,3-c]pyridazin-3-yl 2-substituted lH-pyrimido[5,4-b][l,4]oxazin-7(6H)-one 2-substituted4-chloro-lH-pyrimido[5,4-b][l,4]oxazin-7(6H)-one 2-substituted 5,6-dihydropyrido [2,3 -d]pyrimidin-7( 1 H)-one 2-substituted 4-chloro-5,6-dihydropyrido[2,3-d]pyrimidin-7(lH)-one 2-substituted 4-methyl-5,6-dihydropyrido[2,3-d]pyrimidin-7(lH)-one 2-substituted 4-methyloxy-5,6-dihydropyrido[2,3-d]pyrimidin-7(lH)-one 7-substituted 2H-chromen-2-one 7-substituted 2H-pyrano[2,3-b]pyridin-2-one 4-chloro-6,7-dihydro-5H-pyrano[2,3-d]pyrimidin-2-yl 8-substituted 2H-pyrido[ 1,2-a]pyrimidin-2-one -10- 6,7-dihydro-5H-pyrano[2,3-d]pyrimidin-2-yl) 5 -chloro-1 -benzothiophen-2-yl 6-chloro-1 -benzothiophen-2-yl 1-benzothiophen-5-yl 1 -methyl- 1H-1,2,3-benzotriazol-6-yl imidazo[2,1-b][l ,3]thiazol-6-yl 4-methyl-3,4-dihydro-2H-1,4-benzoxazin-7-yl 1-methy-1 H-indol-2-yl lH-pyrimido[5,4-b][ 1,4]thiazin-7(6H)-one-2-yl [l,2,5]thiadiazolo[3,4-6]pyridine-6-yl 4-fluoro- lH-benzimidazol-2-yl 3,4-dihydro-2H-chromen-7-yl 2,3-dihydro-1 -benzofuran-6-yl 3,4-dihydro-2H-chromen-6-yl 6-chloro-23-dihydro-l,4-benzodioxin-7-yl 7-chloro-3,4-dihydro-2H-pyrido[3,2-6][l,4]oxazine-6-yl 7-chloro-3,4-dihydro-2H-pyrido[3,2-b] [ 1,4]thiazine-6-yl 3,4-dihydro-2H-pyrido [3,2-b] [ 1,4]thiazin-6-yl 5-fluoro-2,3-dihydro-l ,4-benzodioxin- 7-yl 5-fluoro-2,3-dihydro-l,4-benzodioxin-6-yl 8-fluoro-2H-l,4-benzoxazin-3(4H)-one-6-yl 8-fluoro-3,4-dihydro-2H-1,4-benzoxazin-6-yI 7,8-difluoro-3,4-dihydro-2H-1,4-benzoxazin-6-yl 6,7-dihydro-5H-thieno[3,2-6]pyran-2-yl 5 -methyl-2,3 -dihydro-1,4-benzodioxin-7-yl 4-oxo-2,3,4,5-tetrahydro-1,5-benzothiazepin-7-yl 3,4-dihydro-2H-1,4-benzothiazine-6-yl 2,3,4,5-tetrahydro-l,5-benzothiazepine-7-yl 7-fluoro-3,4-dihydro-2H-l,4-benzoxazine-6-yl xo vo xc TO XO X/X XXX XO xxxx^xx^yxo - 1 1 - XX> XX: /YV° H H O OH XXSJCIXISTC1. N s cr XX. H "xx? XO xo N . ^ U / Nl>vx^V *>T O' "V^o' ^ V ^ N ^ N ^ O H Kk ^N^ ^O xx!r w xovvy w CI Y^ T^ N. ^O I CI -O XX) XX) rx / -12- ^ yp -co ^ x0» Xx) x&° XO o —> is the point of attachment especially 3 -oxo-3,4-dihydro-2H-pyrido [3,2-b] [ 1,4]oxazin-6-yl 3-oxo-3,4-dihydro-2H-pyrido[3,2-b][l,4]thiazin-6-yl 2,3-dihydro-[l,4]dioxino[2,3-c]pyridin-7-yl [ 1,3]oxathiolo[5,4-c]pyridin-6-yl 6-fluoro-2,3-dihydro-l,4-benzodioxin-7-yl 2,3-dihydro[ 1,4]oxathiino[2,3-c]pyridin-7-yl 3,4-dihydro-2H-pyrano[2,3-c]pyridin-6-yl 5-fluoro-2,3-dihydro-l,4-benzodioxin-7-yl 5-carbonitro-2,3-dihydro-1,4-benzodioxin-7-yl 2,3-dihydro-benzo[l,4]dioxin-6-yl xxyxxyr® xr:> xoxx: xo ^O i?o xc F CN is the point of attachment When used herein, the term "alkyl" includes groups having straight and branched chains, for instance, and as appropriate, methyl, ethyl, n-propyl, iso-propyl, n-butyl, isobutyl, sec-butyl, t-butyl, pentyi and hexyl. The term 'alkenyl' should be interpreted accordingly. Halo or halogen includes fluoro, chloro, bromo and iodo. Haloalkyl moieties include 1-3 halogen atoms. - 1 3 - Compounds within the invention contain a heterocyclyl group and may occur in two or more tautomeric forms depending on the nature of the heterocyclyl group; all such tautomeric forms are included within the scope of the invention. Some of the compounds of this invention may be crystallised or recrystallised from solvents such as aqueous and organic solvents. In such cases solvates may be formed. This invention includes within its scope stoichiometric solvates including hydrates as well as compounds containing variable amounts of water that may be produced by processes such as lyophilisation. Furthermore, it will be understood that phrases such as "a compound of formula (I) or a pharmaceutically acceptable salt or N-oxide thereof are intended to encompass the compound of formula (I), an N-oxide of formula (I), a pharmaceutically acceptable salt of the compound of formula (I), a solvate of formula (I), or any pharmaceutically acceptable combination of these. Thus by way of non-limiting example used here for illustrative purpose, "a compound of formula (I) or a pharmaceutically acceptable salt thereof may include a pharmaceutically acceptable salt of a compound of formula (I) that is further present as a solvate. Since the compounds of formula (I) are intended for use in pharmaceutical compositions it will readily be understood that in particular embodiments they are provided in substantially pure form, for example at least 60% pure, more suitably at least 75% pure and particularly at least 85%, especially at least 98% pure (% are on a weight for weight basis). Impure preparations of the compounds may be used for preparing the more pure forms used in the pharmaceutical compositions; these less pure preparations of the compounds should contain at least 1%, more suitably at least 5% and more particularly from 10% of a compound of the formula (I) or pharmaceutically acceptable salt and/or N-oxide thereof. Particular compounds according to the invention include those mentioned in the examples and their pharmaceutically acceptable N-oxides, salts and solvates. Pharmaceutically acceptable salts of the above-mentioned compounds of formula (I) include the acid addition or quaternary aminonium salts, for example their salts with mineral acids e.g. hydrochloric, hydrobromic, sulphuric nitric or phosphoric acids, or organic acids, e.g. acetic, fumaric, succinic, maleic, citric, benzoic, p-toluenesulphonic, methanesulphonic, naphthalenesulphonic acid or tartaric acids. Compounds of formula (I) may also be prepared as the N-oxide. The invention extends to all such derivatives. Certain of the compounds of formula (I) may exist in the form of optical isomers, e.g. diastereoisomers and mixtures of isomers in all ratios, e.g. racemic mixtures. The invention includes all such forms, in particular the pure isomeric forms. For example the invention includes enantiomers and diastereoisomers at the attachment point of NR.2 and -14- R3. The different isomeric forms may be separated or resolved one from the other by conventional methods, or any given isomer may be obtained by conventional synthetic methods or by stereospecific or asymmetric syntheses. Certain compounds of formula (I) may also exist in polymorphic forms and the invention includes such polymorphic forms. In a further aspect of the invention there is provided a process for preparing compounds of formula (I) where Z2 is nitrogen, and pharmaceutically acceptable salts and/or N-oxides thereof, which process comprises reacting a compound of formula (IIA): L (IIA) in which L is a leaving group or -A(Q1)(Q2), where Ql and Q2 are both attached to the same carbon atom on A, Ql is H and Q2 is N(R20)R2' or Q1 and Q2 together form ethylenedioxy or oxo, R20 is UR5 or a group convertible thereto and R2' is R2 or a group convertible thereto, A, R1b, R2, U and R5, are as defined in formula (I), with (i) ethyl bromoacetate followed by cyclisation and oxidation or (ii) ethyl oxoacetate followed by cyclisation, to give a compound of formula (IIIA): FT- ^ N (IIIA) and thereafter optionally or as necessary converting L to -A-NR2-UR5, interconverting any variable groups, and/or forming a pharmaceutically acceptable salt and/or N-oxide thereof. The reaction variant (i) is a selective alkylation with ethyl bromoacetate under basic conditions (such as potassium carbonate) (see Yoshizawa, H. et al., Heterocycles (2004), 63(8), 1757-1763 for an example of this selectivity in the alkylation of 2,3- diaminopyridines), thermal cyclisation under strong basic conditions (such as potassium t-butoxide) and then oxidation with manganese dioxide under conventional conditions (see for examples Smith, M.B.; March, J.M. Advanced Organic Chemistry, Wiley- Interscience 2001). The reaction variant (ii) may be carried out in toluene and the cyclisation effected under strongly basic conditions (such as potassium t-butoxide). -15- L may be a hydroxy group which can be oxidised to the aldehyde by conventional means such as l,l,l-tris-(acetyloxy)-l,l-dihydro-l,2-benziodooxol-3-(lH)-one for reductive alkylation with HA-N(R20)R2' under conventional conditions (see for examples Smith, M.B.; March, J.M. Advanced Organic Chemistry, Wiley-Interscience 2001). Alternatively L may be bromo which can be alkylated with HA-N(R20)R2' UNder conventional conditions. Where Q1 and Q2 together form ethylenedioxy the ketal may be converted to the ketone (Q1 and Q2 together form oxo) by conventional acid hydrolysis treatment with eg aqueous HC1 or trifluoroacetic acid and the conversion to NR2UR5 by conventional reductive alkylation with amine NHR2'R20 (see for example Nudelman, A., et al, Tetrahedron 60 (2004) 1731-1748) and subsequent conversion to the required substituted amine, or directly with NHR2URS, such as with sodium triacetoxyborohydride in dichloromethane/methanol. Conveniently one of R20 and R2' is an N-protecting group, such as such as tbutoxycarbonyl, benzyloxycarbonyl or 9-fluorenylmethyloxycarbonyI. This may be removed by several methods well known to those skilled in the art (for examples see "Protective Groups in Organic Synthesis, T.W. Greene and P.G.M. Wuts, Wiley- Interscience, 1999), for example conventional acid hydrolysis with, for example trifluoroacetic acid or hydrochloric acid. The invention further provides compounds of formula (IIIA) in which L is -A-N(R20)R2' and R20 is hydrogen. The free amine of formula (IIIA) in which R20 is hydrogen may be converted to NR2UR5 by conventional means such as amide formation with an acyl derivative R5COW, for compounds where U is CO or, where U is CH2, by alkylation with an alkyl halide R5CH2-halide in the presence of base, acylation/reduction with an acyl derivative R5COW or reductive alkylation with an aldehyde R5CHO under conventional conditions (see for examples Smith, M.B.; March, J.M. Advanced Organic Chemistry, Wiley- Interscience 2001). The appropriate reagents containing the required R5 group are known compounds or may be prepared analogously to known compounds, see for example WO02/08224, WO02/50061, WO02/56882, WO02/96907, WO2003087098, WO2003010138, WO2003064421, WO2003064431, WO2004002992, WO2004002490, WO2004014361, WO2004041210,WO20040969825 WO2002050036, WO2004058144, WO2004087145, WO06002047, WO06014580, WO06010040, WO06017326, WO06012396, WO06017468, WO06020561, WO2004/035569, WO2004/089947, WO2003082835, WO06002047, WO06014580, WO06010040, WO06017326, WO06012396, WO06017468, WO06020561, WO06132739, WO06134378, WO06137485, WO06081179, WO06081264, WO06081289, WO06081178, -16- WO0608I182, WO07016610, WO07081597, WO07071936, WO07115947, WO07118130, WO07122258, WO08006648, WO08003690, WO08009700, WO2007067511 andEP0559285. Where R5 contains an NH group, this may be protected with a suitable Nprotecting group such as t-butoxycarbonyl, benzyloxycarbonyl or 9- fluorenylmethyloxycarbonyl during the coupling of the R5 derivative with the free amine of formula (IIB). The protecting group may be removed by conventional methods, such as by treatment with trifluoroacetic acid. In a further aspect of the invention there is provided a process for preparing compounds of formula (I) where Z1 is nitrogen, and pharmaceutically acceptable salts and/or N-oxides thereof, which process comprises reacting a compound of formula (IIB): L (IIB) in which L is a leaving group or -A(Q})(Q2), where Q1 and Q2 are both attached to the same carbon atom on A, Q1 is H and Q2 is N(R20)R2' o r Q1 ANd Q2 together form ethylenedioxy or oxo, R20 is UR5 or a group convertible thereto and R2' is R2 or a group convertible thereto, A, R1a, R2, U and R5, are as defined in formula (I), with (i) ethyl bromoacetate followed by cyclisation and oxidation or (ii) ethyl oxoacetate followed by cyclisation, to give a compound of formula (IIDB): L ^N' " ^ ^R1a (1MB) and thereafter optionally or as necessary converting L to -A-NR2-UR5, interconverting any variable groups, and/or forming a pharmaceutically acceptable salt and/or N-oxide thereof. The reaction and subsequent transformations is carried out as for the preparation of compounds of formula (IIIA). -17- The invention further provides compounds of formula (IIIB) in which L is -AN( R20)R2' and R20 is hydrogen. Compounds of formula (IIB) (L= -A(Ql)(Q2)) may be prepared by Scheme 1: Scheme 1 c ' l J N02 ^YCI ^ (2) a\sa' A .. IN NH, HN. ,N. ,CI H20 HN N NO^V (6) (5) Chloropyridine (2) can be reacted with allylamine to give (3) which can then be cyclised with bromine generating pyridone (4) after a hydrolytic workup (see Schmid, S et al, Synthesis, 2005 (18), 3107). Displacement with H-A(Q1)(Q2) gives (5) and hydrogenation of (5) over Pd/C can give amine (6). Compounds of formula (IIIA) may be prepared by Scheme 2 utilising compounds of formula (TVA): OMe^ Jvk ^NH R i a , ^ ^N H^ (IVA) The starting material may be prepared by reduction of compound (3) from Scheme 1 with sodium methoxide and then reduction with tin (IT) chloride or sodium dithionite. Cyclisation of (IVA) with propiolate esters gives (19) (Scheme 2) (see Kalyanam, N. et al, Indian Journal of Chemistry, Section B: Organic Chemistry Including Medicinal Chemistry (1992), 31B(7), 415-420). Standard protection to give (20) then cyclisation with bromine (see Schmid, S et al, Synthesis, 2005 (18), 3107) may then access bromomethyl analogue (21) which may be deprotected with TFA to (22) and oxidised with hydrogen peroxide or manganese (II) oxide to give (23) (see Sakata, G., Heterocycles (1985), 23(1), 143-51). -18- Scheme 2 ,CX .N. .N^.0 / V V ^ "'° (20) BOC (19) Br O - ^ Z - ' V / N \ ^ ° O^ ^Nk ^Nk ^O N (21) BOC Br 0 , ; ^ v / N \ / N v v ^ 0 p,/kAM (23) Compounds of formula (I) in which Z1 and Z2- are both CH may be prepared by Scheme 3: Scheme 3 -19- R XT* (D O. M~ M (a) Y ^ *. AABr ° (2) (6) W (g) R'~ ^ v ~R" (7) (8) Lis-A(Ql)(Q2) (a) n-butyl lithium, dibromoethane (b) tris(dibenzylideneacetone)dipalladiiun(0), N,Ndicyclohexylmethylamine, bis(tri-t-butylphosphine)palladium(0), butyl acrylate (c) hydrogen, palladium/charcoal followed by acid treatment (HC1) (d) see text (e) amine H-A(Q1)(Q2), heat (f) methanesulphonic anhydride, triethylamine then KI (g) 2,3-dichloro-5,6-dicyano-l,4-benzoquinone (h) i) DMF, heat, ii) methanesalfbnyl chloride, triethylarmine, iii) amine H-A(Q1)(Q2), heat Metallation of (1) (commercially available) with n-butyl lithium followed by bromination with dibromoethane affords bromopyridine derivative (2) (see Zhichkin, P. etal, Synlett (2006), (3), 379-382 for examples of this type of metallation chemistry). Heck reaction of (2) using palladium catalysis (see Sydorenko, N, et al, Organic & Biomolecular Chemistry (2005), 3(11), 2140-2144 for an example of this type of catalysis in a Heck reaction) gives acryate (3). Hydrogenation of the double bond of (3) followed by acid treatment to remove the pivalate residue and effect lactamisation yields the bicyclic lactam (4). Conversion to the epoxide (5) can be effected in a number of ways - reaction with epichlorohydrin under basic conditions affords racemic epoxide. -20- Reaction with (commercially available) R or S-glycidyl nosylate ((2R)- or (2S)-2- oxiranylmethyl 3-nitrobenzenesulfonate) or (2R)- or (2.S)-2-oxiranylmethyl 4- methylbenzenesulfonate, with base eg sodium hydride or potassium t-butoxide, gives the corresponding chiral epoxides. Alternatively, allylation with allyl bromide under basic conditions affords the corresponding N-allyl material which can be epoxidised under standard achiral or chiral conditions to give the corresponding achiral or chiral epoxides. The epoxide(s) (5) may be opened with amine H-A(Q1)(Q2) such as 1,1-dimethylethyI 4- piperidinylcarbamate by heating in DMF to afford (6) which can then be cyclised with methanesulphonic anhydride to give (7). Alternatively, the epoxide (5) may be opened and cyclised directly with heating, to afford (7) (L=OH). Oxidation to (8) may be carried out by oxidation with 2,3-dichloro-5,6-dicyano-l,4-benzoquinone (DDQ). Subsequent conversion to compounds of formula (I) may be carried out as generally described herein. In particular, conversion of L to A(Q1)(Q2) may be carried out on (7) or (8). As a furter variation to Scheme 3, epoxide (5) may be prepared from (2) by first introducing a suitable epoxide precursor group (-CH2-CHOH-CH2OH, protected as a cyclic ester) before carrying out the steps (b) and (c). The invention further provides compounds of formula (8) from Scheme 3 in which L is -A-N(R20)R2' and R20 is hydrogen. Compounds of formula (I) in which Z2 is N may alternatively be prepared by Scheme 4: Scheme 4 -21 - (1) (a) OH OH (2) X (b) R XX, (3) (c) X, O O XV. (6) * CO X xu ° \ / N \ ^ ' N \ v i S > 0 0 ^ ^N^ -N. >j.O CBZ (7) xu xu, N' I CBZ (7a) (g) OMs OMs (h) OMs 0 ^ s / N \ ^ N \ ^ 0 (i) (9) CBZ (10) H R (11) (a) 2-amino-1,3-propanediol (b) dimethoxypropane, p-toluenesulfonic acid (c) hydrogen, palladium/charcoal (d) etfeyl bjomoacetate, potassium caibonate (e) sodium hydride (f) Benzyl chloroformate (g) Aqueous acid (h) Methane sulphonic anhydride (i) Hydrogen, palladium/charcoal (j) Mn02 - 2 2 - Reaction of nitropyridine (1) with 2-amino-l,3-propanediol affords diol (2) which is protected as acetal (3). Reduction of the nitro group gives amine (4) which is alkylated to yield ester (5). Cyclisation can be effected with sodium hydride to give (6). This is protected with a carboxybenzyl (CBz) group (7) then cleaved to give the diol (8). Cyclisation with methanesulphonic anhydride affords the mesylate (9), then hydrogenolysis of the CBz group (10) and subsequent oxidation with manganese(II)oxide gives the key dione intermediate mesylate (11). The order of steps may be changed to go via (7a). The mesylate (11) may be converted to the compound of formula (I) as generally described herein. Chiral compounds of formula (I) in which Z^ is N may alternatively be prepared by Scheme 4a: Scheme 4a .OBn ^OBn OTBS i rOBn i f i (c) I 9" ?TBS ^OBn OBn U s / 'W-co.a U*^ (7) (6) (5) (9). (h) XXNJ — xxj — xxj (8) (9) (10) Bn = benzyl (a) EtOH, reflux, (b) TBS-C1, (c) Zinc, acetic acid, (d) ethyl bromoacetate, potassium carbonate (e) NaH, (f) hydrogen, palladium/charcoal,(g) Mn02, (h) methanesulfonic anhydride (i) TFA, (j) methanesulfonic anhydride Reaction of nitropyridine (1) with chiral amine (2) gives intermediate (3). Protection of (3) with tert-butyl-dimethylsilylchloride gives (4). Reduction of the nitro group gives amine (5), which is alkylated to yield ester (6). Cyclisation of (6) can be effected with sodium hydride and then treatment with hydrogen over a palladium/charcoal cataiyst gives intermediate (7). Oxidation with manganese(II)oxide and treatment with methanesulfonic anhydride gives (8). This intermediate can be deprotected with TFA to give (9) and reacted with methanesulfonic anhydride to give - 2 3 - (10). The mesylate (10) formed may then be converted to the compound of formula (I) as generally described herein. Compounds of formula (I) in which Z1 is N may alternatively be prepared by Scheme 5: Scheme 5 I | | | R ^ ^ - ^ N C , R^ A A N H 2 ^M^OA Ml (2) (3) (4) H II O Ck M^ J L ^O O (d) J Y Y T „ I Ks? r (g) r 0 - S f 0 y-L 0 ^ > k ^ I V ^O ;xir^ xcc^xcc err6 rj^Y^o^^o rr^V^o^^o O) "^ (10) x fl) OH 0) O V I f** O^ M^. M^ ^.0 l 1 - •• {' ' O^.N^.N^.0 - l I * °VrNr O ^ - N v ^ N , . -O I I "-'•V > T (13) (11) (12) Lis-ACQ1 XQ2) (a) NH3/MeOH (b) hydrogen, palladium/charcoal (c) ) ethyl bromoacetate, potassium carbonate (d) potassium tert-butoxide (e) CBzCl (f) NaH, ($-glycidyl nosylate (g) DMF, heat (h) methanesulfonyl chloride (i) amine H-A(Q1)(Q2), heat (j) hydrogen, palladium/charcoal then MnC>2 Reaction of nitropyridine (1) with aminonia affords nitro-pyridine (2) which is reduced to bis-aniline (3). Alkyation with ethyl bromoacetate followed by cyclisation with potassium fert-butoxide gives (5). This is protected with a carboxybenzyl group to give (6) which can then be reacted with (commercially available) S-glycidyl nosylate -24- ((2S)-2-oxiranylmethyl 3-nitrobenzenesulfonate) to give (7). Cyclisation under thermal conditions gives (8). Mesylation, displacement with an appropriate amine, hydrogenolysis of the CBz group (10) and subsequent oxidation with manganese(II)oxide gives (13). Alternatively hydrogenolysis of the CBz group (10) and subsequent oxidation with manganese(II)oxide, followed by mesylation and displacement with an appropriate amine also gives (13). This may be converted to the compound of formula (I) as generally described herein. Compounds of formula (I) in which Z1 and Z3 are both N may be prepared by Scheme 6: Scheme 6 T>YxV XX ^ N CI H TXT N N H (4) N a (3) (a) chloroacetyl chloride (b) aminonia (c) Boc-glycine (d) HC1 Compound (1) (Bioorganic & Medicinal Chemistry Letters (2005), 15(24), 5446- 5449) is converted to (2) by acylation with chloroacetyl chloride followed by treatment with aminonia to give (4). Alternatively (1) may be converted to (3) by coupling with Boc- glycine followed by acidic deprotection to give (4). Compound (4) may then be converted to a compound of formula (I) by analogy with the conversion of compound (5) of Scheme 5. Interconversions ofRl a,Rlb,R2,AandR5 are conventional. In compounds which contain an optionally protected hydroxy group, suitable conventional hydroxy protecting groups which may be removed without disrupting the remainder of the molecule include acyl and alkylsilyl groups. N-protecting groups are removed by conventional methods. Interconversion of R1a and R1b groups maybe carried out conventionally, on compounds of formula (I). For example R1a or R1b methoxy is convertible to R1a or RlD hydroxy by treatment with lithium and diphenylphosphine (general method described in Ireland et al, J. Amer. Chem. Soc, 1973, 7829) or HBr. Alkylation of the hydroxy group with a suitable alkyl derivative bearing a leaving group such as halide, - 2 5 - yields R1a or R1b substituted alkoxy. Rla or Rlb halo such as bromo may be converted to cyano by treatment with copper (I) cyanide in N,N-dimethylformamide. Rla or R1b carboxy may be obtained by conventional hydrolysis of R l a or Rib cyano, and the carboxy converted to hydroxymethyl by conventional reduction. Compounds of formula HA-N(R20)R2' are known compounds or may be prepared analogously to known compounds, see for example WO2004/035569, WO2004/089947, WO02/08224, WO02/50061, WO02/56882, WO02/96907, WO2003087098, WO2003010138, WO2003064421, WO2003064431, WO2004002992, WO2004002490, WO2004014361, WO2004041210,WO2004096982, WO2002050036, WO2004058144, WO2004087145, WO2003082835, WO2002026723, WO06002047 and WO06014580, WO06134378, WO06137485, WO07016610, WO07081597, WO07071936, WO07115947, WO07118130, WO07122258, WO08006648, WO08003690 and WO08009700. Further details for the preparation of compounds of formula (I) are found in the examples. The antibacterial compounds according to the invention may be formulated for administration in any convenient way for use in human or veterinary medicine, by analogy with other antibacterials/antitubercular compounds. The pharmaceutical compositions of the invention may be formulated for administration by any route and include those in a form adapted for oral, topical or parenteral use and may be used for the treatment of bacterial infection including tuberculosis in mammals including humans. The compositions may be in the form of tablets, capsules, powders, granules, lozenges, suppositories, creams or liquid preparations, such as oral or sterile parenteral solutions or suspensions. The topical formulations of the present invention may be presented as, for instance, ointments, creams or lotions, eye ointments and eye or ear drops, impregnated dressings and aerosols, and may contain appropriate conventional additives such as preservatives, solvents to assist drug penetration and emollients in ointments and creams. The formulations may also contain compatible conventional carriers, such as cream or ointment bases and ethanol or oleyl alcohol for lotions. Such carriers may be present as from about 1% up to about 98% of the formulation. More usually they will form up to about 80% of the formulation. Tablets and capsules for oral administration may be in unit dose presentation form, and may contain conventional excipients such as binding agents, for example syrup, acacia, gelatin, sorbitol, tragacanth, or polyvinylpyrrolidone; fillers, for example lactose, sugar, maize-starch, calcium phosphate, sorbitol or glycine; tabletting lubricants, -26- for example magnesium stearate, talc, polyethylene glycol or silica; disintegrants, for example potato starch; or acceptable wetting agents such as sodium lauryl sulphate. The tablets may be coated according to methods well known in normal pharmaceutical practice. Oral liquid preparations may be in the form of, for example, aqueous or oily suspensions, solutions, emulsions, syrups or elixirs, or may be presented as a dry product for reconstitution with water or other suitable vehicle before use. Such liquid preparations may contain conventional additives, such as suspending agents, for example sorbitol, methyl cellulose, glucose syrup, gelatin, hydroxyethyl cellulose, carboxymethyl cellulose, aluminium stearate gel or hydrogenated edible fats, emulsifying agents, for example lecithin, sorbitan monooleate, or acacia; non-aqueous vehicles (which may include edible oils), for example almond oil, oily esters such as glycerine, propylene glycol, or ethyl alcohol; preservatives, for example methyl or propyl p-hydroxybenzoate or sorbic acid, and, if desired, conventional flavouring or colouring agents. Suppositories will contain conventional suppository bases, e.g. cocoa-butter or other glyceride. For parenteral administration, fluid unit dosage forms are prepared utilizing the compound and a sterile vehicle, water being preferred. The compound, depending on the vehicle and concentration used, can be either suspended or dissolved in the vehicle. In preparing solutions the compound can be dissolved m water for injection and filter sterilised before filling into a suitable vial or ampoule and sealing. Advantageously, agents such as a local anaesthetic, preservative and buffering agents can be dissolved in the vehicle. To enhance the stability, the composition can be frozen after filling into the vial and the water removed under vacuum. The dry lyophilized powder is then sealed in the vial and an accompanying vial of water for injection may be supplied to reconstitute the liquid prior to use. Parenteral suspensions are prepared in substantially the same manner except that the compound is suspended in the vehicle instead of being dissolved and sterilization cannot be accomplished by filtration. The compound can be sterilised by exposure to ethylene oxide before suspending in the sterile vehicle. Advantageously, a surfactant or wetting agent is included in the composition to facilitate uniform distribution of the compound. The compositions may contain from 0.1% by weight, preferably from 10-60% by weight, of the active material, depending on the method of administration. Where the compositions comprise dosage units, each unit will preferably contain from 50-1000 mg of the active ingredient. The dosage as employed for adult human treatment will preferably range from 100 to 3000 mg per day, for instance 1500 mg per day depending on the route and frequency of administration. Such a dosage corresponds to about 1.5 to about 50 mg/kg per day. Suitably the dosage is from 5 to 30 mg/kg per day. -27- The compound of formula (I) may be the sole therapeutic agent in the compositions of the invention or a combination with other antibacterials including antirubercular compounds. If the other antibacterial is a ß-lactam then a ß-lactamase inhibitor may also be employed. Compounds of formula (I) may be used in the treatment of bacterial infections caused by a wide range of organisms including both Gram-negative and Gram-positive organisms, such as upper and/or lower respiratory tract infections, skin and soft tissue infections and/or urinary tract infections. Compounds of formula (I) may be also used in the treatment of tuberculosis caused by Mycobacterium tuberculosis. Some compounds of formula (I) may be active against more than one organism. This may be determined by the methods described herein. The following examples illustrate the preparation of certain compounds of formula (I) and the activity of certain compounds of formula (I) against various bacterial organisms including Mycobacterium tuberculosis. -28- Examples and Experimental General Abbreviations in the examples: MS = mass spectrum ES = Electrospray mass spectroscopy LCMS/LC-MS = Liquid chromatography mass spectroscopy HPLC = high performance liquid chromatography rt = room temperature Rf = retention factor Certain reagents are also abbreviated herein. TFA refers to trifluoroacetic acid, THE refers to tetrahydrofuran, Pd/C refers to palladium on carbon catalyst, DCM refers to dichloromethane, MeOH refers to methanol, DMF refers to dimethylformamide, EtOAc refers to ethylacetate, DDQ refers to 2,3-dichloro-5,6-dicyano-l,4-benzoquinone, NaBH(OAc)3 refers to sodium triacetoxyborohydride, Pd2(dba)3 refers to tris(dibenzylideneacetone)dipalladium (0). Proton nuclear magnetic resonance ('HNMR) spectra were recorded at 400 or 250 MHz, and chemical shifts are reported in parts per million (ppm) downfield from the internal standard tetramethylsilane (TMS). Abbreviations forNMR data are as follows: s = singlet, d = doublet, t = triplet, q = quartet, m = multiplet, dd = doublet of doublets, dt = doublet of triplets, td = triplet of doublets, app = apparent, br = broad. J indicates the NMR coupling constant measured in Hertz. CDCI3 is deuteriochloroform and CD3OD is tetradeuteriomethanol. Mass spectra were obtained using electrospray (ES) ionization techniques. All temperatures are reported in degrees Celsius. MP-carbonate refers to macroporous triethylaminonium methylpolystyrene carbonate (Argonaut Technologies). Amberlyst®A21 is a weakly basic, macroreticular resin with alkyl amine functionality, ©Registered trademark of Rohm & Haas Co. AD mix alpha is prepared by mixing potassium osmate (K2OSO4.2H2O) (0.52g), (3a,9R,3,"a,4,"b,9,"R)-9,9'-[l,4-phthalazinediylbis(oxy)]bis[6,-(methyloxy)-10,lldihydrocinchonan] [(DHQ)2PHAL] (5.52g), then adding potassium ferricyanide [K.3Fe(CN)6] (700g) and powdered potassium carbonate (294g). This mixture is stirred in a blender for 30 minutes. This provides approximately 1kg of AD mix alpha, which is commercially available from Aldrich. See K. Barry Sharpless et al, J. Org. Chem., 1992, 57 (10), 2771. AD mix beta is the corresponding mixture prepared with (9S,9'"S)-9,9'- [ 1,4-phthalazinediylbis(oxy)]bis[6'-(methyloxy)-10,11 -dihydrocinchonan] [(DHQD)2PHAL]. Where AD mix alpha/beta is referred to, this is a 1.1 mixture of the alpha and beta mix. Celite® is a filter aid composed of acid-washed diatomaceous silica, and is a trademark of Manville Corp., Denver, Colorado. SCX Cartridge is an ion exchange column coTrtaming strong cation exchange resin ( benzene sulfonic acid) supplied by Varian, USA. -29- Chiralpak IA and Chiralpak AS-H are polysaccharide based chiral HPLC columns (Chiral Technologies Inc.). Chiralpak AS-H column comprise amylose tris [(S)- alphamethylbenzylcarbamate) coated onto 5um silica. Chiralpak IA column comprise silica for preparative column (5 um particle size, 21mm ID x 250mm L ) immobilized with Amylose tris (3,5-dimethylphenylcarbamate). Chiralpak AD and AD-H columns comprise silica for preparative columns (5 urn particle size AD-H and lOum particle size AD, 21mm ID x 250mm L; 20 uM particle size AD, 101 mm ID x 250mm L) coated with Amylose tris (3,5-dimethylphenylcarbamate) (Chiral Technologies USA). Measured retention times are dependent on the precise conditions of the chromatographic procedures. Where quoted below in the Examples they are indicative of the order of elution. Kromasil 5 micron C-18 column (21mm x 250mm) comprises octadecylsilane chemically bonded to 5 micron porous silica gel. As will be understood by the skilled chemist, references to preparations carried out in a similar manner to, or by the general method of, other preparations, may encompass variations in routine parameters such as time, temperature, workup conditions, minor changes in reagent amounts etc. Reactions involving metal hydrides including lithium hydride, lithium aluminium hydride, di-isobutylaluminium hydride, sodium hydride, sodium borohydride and sodium triacetoxyborohydride are carried out under argon or other inert gas. Example 1 l-({4-[(2,3-Dihydro[l,4]dioxino[2,3-c]pyridin-7-ylmethyl)amino]-lpiperidinyl} methyl)-l,2-dihydro-3ff,8fl-2a^,8a-triazaacenaphthylene-3,8-dione dihydrochloride ^ N NH 6 XXX (a) 6-Chloro-3-nitro-N-2-propen-l-yl-2-pyridinamine This was prepared by a modification of the method of Schmid, S., et al, Synthesis (2005), (18), 3107-3118. A solution of 2,6-dichloro-3-nitropyridine (8.0g, 41.45mmol) in anhydrous dichloromethane (180ml) was cooled to -15°C, under argon. Triethylamine (6.0ml, 43mmol) was added and then allylamine (3.23ml, 43mmol) was added in small portions over 3 hours, keeping the temperature at -15°C. The reaction mixture was stirred overnight during which time it warmed to room temperature. The reaction mixture was washed with 0.2M aqueous citric acid (100ml), saturated aqueous NaHCC«3 solution (100ml), passed through a hydrophobic frit and evaporated to a yellow oil which was -30- purified by chromatography on silica eluting with a 0 to 50% ethyl acetate in hexane giving a yellow solid (7.49g, 85%). C8H8ClN3O2 requires 213, MS (ES+) m/z 214, 216(MH+). (b)3-(Bromomethyl)-8-nitro-2,3-dihydroimidazo[l,2-a]pyridin-5(lH)-one This was prepared by a modification of the method of Schmid, S., et al, Synthesis (2005), (18), 3107-3118. A solution of 6-chloro-3-nitro-N-2-propen-l-yl-2-pyridinamine (20g, 93.6mmol) in chlorobenzene (500ml) was treated with a solution containing bromine (4.75ml, 92.7mmol) in chlorobenzene (100ml), dropwise over 4.5 hours, keeping T temperature for 18 hours and diluted with hexane (200ml) and then the reaction mixture was then pored into hexane (1000ml). After 15 minutes the orange precipitate was collected by filtration and washed with hexane (250ml) to give 26.6g of an orange solid (3-(bromomethyl)-5-chloro-8-nitro-2,3-dihydroimidazo[l,2-a]pyridin-l-ium bromide). This intermediate was added, over 45 minutes, to a rapidly stirred mixture of saturated aqueous NaHCO3 solution (1000ml) and ethyl acetate (500 ml). The bright red mixture was stirred for 1 hour, diluted with ethyl acetate (200ml) and the layers were separated. The aqueous layer was washed with ethyl acetate (200ml) and the organic extracts were combined, dried (anhydrous sodium sulphate), filtered and evaporated to give the product as a brown solid (18.3g, contains 40% 6-bromo-3-(bromomethyl)-8-nitro-2,3- dihydroimidazo[ 1,2-a]pyridin-5(lH)-one). C8H8BrN303 requires 273, MS (ES+) m/z 274, 276(MH+). (c) 1,1 -Dimethylethyl {1 -[(8-nitro-5-oxo-1,2,3,5-tetrahydroimidazo[ 1,2-a]pyridin-3- yl)methyl]-4-piperidinyl}carbamate A suspension of a 3:2 mixture of 3-(bromomethyl)-8-nitro-2,3- dihydroimidazo[ 1,2-a]pyridin-5(1H)-one and 6-bromo-3-(bromomethyl)-8-nitro-2,3- dihydroimidazo[l,2-a]pyridin-5(lH)-one (18.2g) was treated with 1,1-dimethylethyl 4- piperidinylcarbamate (26.6g, 132.8mmole) in acetonitrile (900ml) then pyridine (10.7ml, 132mmol). The mixture was heated at 60°C under argon for 17 hours and then heated at 70°C for 2 hours, cooled and evaporated to about half the volume. The thick yellow precipitate was removed by filtration and washed well with diethyl ether. The filtrate was evaporated to dryness and the residue partitioned between chloroform (500ml) and water (200ml). The undissolved material was removed by filtration and washed with chloroform (100ml). The layers in the filtrate were separated and the aqueous layer was washed with chloroform (200ml). The combined organic extracts were passed through a hydrophobic frit and evaporated to a dark yellow gum which was chromatographed eluting with 0 to 100% ethyl acetate in hexane then 0 to 30% methanol in ethyl acetate to give a yellow solid (10.98g). C18H27N505 requires 393, MS (ES+) m/z 394(MH+). (d) 1,1 -Dimethylethyl {1 -[(3,8-dioxo-1,2,5 a,8b-tetrahydro-3H,8H-2a,5,8atriazaacenaphthylen- 1 -yl)methyl]-4-piperidinyl} carbonate - 3 1 - A suspension of 1,1-dimethylethyl {l-[(8-nitro-5-oxo-l,2,3,5- tetrahydroimidazo[ 1,2-a]pyridin-3-yl)methyl]-4-piperidinyI}carbamate (2.0g, 5.08mmol) and anhydrous potassium carbonate (700mg, 5.06mmol) in absolute alcohol (150ml) was hydrogenated at atmospheric pressure in the presence of 10%Pd on C (lg) for 4 hours. The reaction was filtered through Keiselguhr, washed through with ethanol (100ml) and the dark purple mixture was reacted immediately by treating with anhydrous potassium carbonate (1.4g, 10mmol) and ethyl bromoacetate (550ul, 4.95mmol) and stirred at room temperature for 20 hours and then heated at 60°C for 30 minutes. After 45 minutes a further 0.25ml of ethyl bromacetate was added and heated at 60°C for 1.5 hours. 0.25ml of ethyl bromacetate was added and the reaction was again heated at 60°C for l.hour. The reaction was filtered through Keiselguhr and evaporated to dryness. The mixture was azeotroped with chloroform and then chromatographed eluting with 0 to 100% ethyl acetate in hexane and then with 0 to 20% methanol in ethyl acetate. A second purification eluting with 0 to 50% methanol in ethyl acetate gave a dark gum (37mg, 1.6%). C20H27N5O2 requires 401, MS (ES+) m/z 402(MH+). (e) l-[(4-Amino-l-piperidinyl)methyl]-1,2,5a,8b-tetrahydro-3H,8H-2a,5,8atriazaacenaphthylene- 3,8-dione A solution of 1,1-dimethylethyl {l-[(3,8-dioxo-l,2,5a,8b-tetrahydro-3H,8H- 2a,5,8a-triazaacenaphthylen-l-yl)methyl]-4-piperidinyl}carbamate (37mg, 0.092mmol) in anhydrous dichloromethane (2ml) was treated with TFA (1ml) and stirred at room temperature for 1 hour, evaporated to dryness, mixed with anhydrous dichloromethane and evaporated to a dark gum. This gum was dissolved in 1:1 dichloromethane methanol (10ml) and treated with MP-carbonate resin (600mg) and stirred for 1.5 hours. The reaction was filtered and the resin was washed with 1:1 dichloromethane:methanol (30ml) and the filtrate was evaporated to dryness. Purification on a 5g SCX column eluting with a methanol to 2N methanolic aminonia gradient gave the product as a gum. Further evaporation from diethyl ether gave the product as a brown solid (22.8mg, 82%). C15H19N502 requires 301, MS (ES+) m/z 302(MH+) (f) Title compound A solution of l-[(4-amino-l-piperidinyl)methyl]-l,2,5a,8b-tetrahydro-3H,8H- 2a,5,8a-triazaacenaphthylene-3,8-dione (22.8mg, 0.0757mmol) in anhydrous dichloromethane (3ml) and anhydrous methanol (0.6ml) was treated with 2,3- dihydro[l,4]dioxino[2,3-c]pyridine-7-carbaldehyde (12.5mg, 0.076mmol) (for a synthesis see WO2004058144 Example 2(c) or WO03/087098 Example 19(d)) and stirred, under argon, for 15 minutes and then treated with sodium triacetoxyborohydride (48mg, 0.226mmol) and stirred at room temperature for 17 hours. The reaction was then treated with a further portion of 2,3-dihydro[l,4]dioxino[2,3-c]pyridine-7-carbaldehyde (2mg) and sodium triacetoxyborohydride (10mg) and the mixture was stirred for 4 hours, treated with saturated aqueous NaHCO3 solution (1ml) and stirred for 10 minutes. The layers were separated and the aqueous layer was washed with 9:1 dichloromethane:methanol -32- (2x1 Oml). The combined organic extracts were passed through a hydrophobic frit and evaporated to a brown gum which was chromatographed eluting with 0 to 30% methanol in dichloromethane to give the free base of the title compound as a yellow gum (20.6mg, 60%). C23H26N6O4 requires 450, MS (ES+) m/z 451(MH+). 1H NMR (250MHz) 8(CDC13) 1.38-1.54 (2H, m), 1.83-1.93 (2H, m), 2.19-2.36 (2H, m), 2.54-2.73 (3H, m), 2.93-2.98 (1H, m), 3.09-3.15 (1H, m), 3.85 (2H, s), 4.26-4.61 (6H, m), 4.96-5.05 (1H, m), 6.33 (1H, d), 6.82 (1H, s), 7.76 (1H, d), 7.87 (1H, s) and 8.10 (1H, s) The free base of the title compound was dissolved in anhydrous dichloromethane (2ml) and anhydrous methanol (0.5ml) and treated with 1M HC1 in diethyl ether (0.5ml). Diethyl ether was added (5ml) and the suspension was cooled. After centrifuging the solvent was removed and the solid was dried to give the title compound as a brown solid (23.5mg). C23H26N604 requires 450, MS (ES+) m/z 451(MH+). Example 2 l-({4-[([1,3]Oxathiolo[5,4-c]pyridin-6-ylmethyl)amino]-lpiperidinyl} methyl)-l,2-dihydro-4H,9H-imidazo[l,2,3-ij]-l,8-naphthyridine-4,9- dione hydrochloride (a) N-(6-Chloro-2-pyridinyl)-2,2-dimethylpropanamide A solution of 6-chloro-2-pyridinamine (13.776g, 107 mmol) in toluene (100ml) and triethylamine (16.28ml, 118 mmol) at 50°C under argon was treated with 2,2- dimethylpropanoyl chloride (13.81ml, 112mmol). The reaction was then stirred at 50°C for 4h and then at rt for 18h. 2M HC1 (200ml) was then added and the mixture was extracted with diethyl ether (3 x 500ml). The organic extracts were dried (MgSO4), filtered and evaporated to give the product as a brown solid (21.005g, 92%). MS (ES+) m/z 213/215 (MH+). (b) N-(3-Bromo-6-chloro-2-pyridinyl)-2,2-dimethylpropanamide A solution of N-(6-chloro-2-pyridinyl)-2,2-dimethylpropanamide (4.83g, 22.7 mmol) in THF (40ml) at -78 °C under argon was treated with n-butyl lithium (20ml, 2.5M in Hexanes, 50 mmol) over 10 min and then allowed warm to 0°C, stirred at 0°C for 3h and then recooled to -78 °C. The reaction was then treated dropwise with dibromoethane (2.057ml, 23.9 mmol) and the reaction was allowed warm to rt and stirred at rt for 0.5h. The reaction was then treated with water (5ml), stirred at rt for 5min, treated with more water (500ml) and extracted with diethyl ether (3 x 500ml). The organic extracts were dried (MgS04), filtered, evaporated and the residue chromatographed (0-25% ethyl acetate:Hexane) to give the product as a yellow solid (3.489g, 53%). -33- MS (ES+) m/z 291/293/295 (MH+). (c) N-(3-Bromo-6-chloro-2-pyridinyl)-2,2-dimethyl-N-2-propen-l-yrpropanamide A solution of N-(3-bromo-6-chloro-2-pyridinyl)-2,2-dimethylpropanamide (2.305g, 7.907 mmol) in DMF (40ml) at 0°C under argon was treated with sodium hydride (0.696g, 17.395 mmol) and then allowed warm to rt over 0.25h, stirred at rt for 0.25h and then treated with allyl iodide (1.61ml, 17.395mmol) and stirred at rt for lh. The reaction was then treated with water (10ml), concentrated to approximately 5ml, treated with more water (200ml) and extracted with DCM (3 x 200ml). The organic extracts were dried (MgSO4), filtered, evaporated and the residue chromatographed (0- 20% ethyl acetate:Hexane) to give the product as a yellow oil which solidified to an off white solid (5.324g, 67%). MS (ES+) m/z 331/333/335 (MH+). (d)N[3-Bromo-6-(methyloxy)-2-pyridinyl]-2,2-dimethyl-N-2-propen-l-ylpropanamide A solution of N-(3-bromo-6-chloro-2-pyridinyl)-2,2-dimethyl-N-2-propen-1 - ylpropanamide (12.388g, 37.370 mmol) in methanol (100ml) at rt under argon was treated with sodium methoxide solution (25% w/v in methanol, 17.76g, 82.212 mmol) and then heated at reflux for 42h. The reaction was then cooled, treated with water (500ml), and extracted with diethyl ether (3 x 200ml). The organic extracts were dried (MgS04), and evaporated to give the product (10.918g, 89%). MS (ES+) m/z 327/329 (MH+). (e) N-[3-Bromo-6-(methyloxy)-2-pyridinyl]-N-(2,3-dihydroxypropyl)-2,2- dimethylpropanamide A solution of N-[3-bromo-6-(methyloxy)-2-pyridinyl]-2,2-dimethyl-N-2-propen- 1-ylpropanamide (1.246g, 3.81 mmol) in tert-butemol (40ml) at rt under argon was treated with water (40ml) and then with AD-mix a (2.86g) and AD-mix P (2.86g) and stirred at rt for 18h. The reaction was then treated with saturated aqueous sodium sulfite (40ml), stirred for 10min, extracted with 20% methanol/DCM (3 x 100ml). The organic extracts were dried (MgSO4), and evaporated to give the crude product (1.728g, 126%) containing residual tert-butanol. MS (ES+) m/z 361/363 (MH+). (f) N-[3-Bromo-6-(methyloxy)-2-pyridinyl]-2,2-dimethyl-N-[(2-oxo-1,3-dioxolan-4- yl)methyl]propanamide A solution of N[3-bromo-6-(methyloxy)-2-pyridinyl]-N-(2,3-dihydroxypropyl)- 2,2-dimethylpropanamide (7.628g, 21.130 mmol) in DCM (100ml) and pyridine (3.407ml, 42.26mmol) at -78°C under argon was treated with a solution of triphosgene (6.27g, 21.130mmol) in DCM (20ml) over 5min and the reaction was then allowed warm to rt and stirred at rt for 30min. The reaction was then carefully treated with saturated sodium bicarbonate solution (200ml), extracted with DCM (3 x 200ml). The organic -34- extracts were dried (MgS04), and evaporated and chromatographed (0-50% ethyl acetate:Hexane) to give the product as a white solid (5.722g, 70%). MS (ES+) m/z 387/389 (MH+). (g) Butyl (2E)-3-[2-{(2,2-dimethylpropanoyl)[(2-oxo-l ,3-dioxolan-4-yl)methyl]amino}- 6-(methyloxy)-3-pyridinyl]-2-propenoate A mixture of N-[3-bromo-6-(methyloxy)-2-pyridinyl]-2,2-dimethyl-N-[(2-oxol, 3-dioxolan-4-yl)methyl]propanamide (5.722g, 14.722 mmol), Pd(PfBu3)2 (151mg, 0.296mmol), Pd2(dba)3 (135mg, 0.149mmol), in 1,4-dioxane (40ml) was treated with N,N'-dicyclohexylmethylamine (3.48ml, 16.265mmol) and n-butyl acrylate (2.54ml, 17.743mmol) and the mixture was then heated at 80°C for lh. The reaction was then cooled, treated with water (200ml), extracted with DCM (3 x 200ml). The organic extracts were dried (MgS04), and evaporated and chromatographed (0-50% ethyl acetate:Hexane) to give the product as a yellow oil (6.156g, 96%). MS (ES+) m/z 435 (MH+). (h) Butyl 3-[2-{(2,2-dimethylpropanoyl)[(2-oxo-l,3-dioxolan-4-yl)methyl]amino}-6- (methyloxy)-3 -pyridinyl]propanoate A solution of butyl (2E)-3-[2-{(2,2-dimethylpropanoyl)[(2-oxo-l,3-dioxolan-4- yl)methyl]arnino}-6-(methyloxy)-3-pyridinyl]-2-propenoate (6.156g, 14.184mmol) in ethanol (200ml) was treated with palladium on carbon (10% paste, 1.23 g) and the mixture was then stirred at rt under 1 atmosphere of hydrogen for 18h. The reaction mixture was then filtered through a thin pad of Celite, eluting with more ethanol (200ml). The organic filtrate was then evaporated to give the product as a yellow oil (6.065g, 98%). MS (ES+) m/z 437 (MH+). (i) l-(2,3-Dihydroxypropyl)-7-(methyloxy)-3,4-dihydro-1,8-naphthyridin-2(1H)-one A solution of butyl 3-[2-{(2,2-dimethylpropanoyl)[(2-oxo-l,3-dioxolan-4- yl)methyl]amino}-6-(methyloxy)-3-pyridinyl]propanoate (6.065g, 13.91 lmmol) in methanol (100ml) was treated with concentrated aqueous HC1 (12M, 50ml) and then heated at reflux for 48h. The reaction mixture was then concentrated to approximately 50ml, neutralised with potassium carbonate and extracted with 20% methanol/DCM (3 x 100ml). The organic extracts were dried (MgS04), and evaporated to give the crude product as a yellow oil (2.325g, 66%). MS (ES+) m/z 279 (MH+). (j)7-(Methyloxy)-l-(2-oxiranylmethyl)-3,4-dihydro-l,8-naphthyridin-2(1H)-one A solution of l-(2,3-dihydroxypropyl)-7-(methyloxy)-3,4-dihydro-l,8- naphthyridin-2(1H)-one (2.325g, 9.226mmol) in DCM (40ml) and triethylamine (1.915ml, 13.839mmol) at 0°C under argon was treated with methanesulfonyl chloride (0.714ml, 9.226mmol) and stirred at 0°C for 0.5h. The reaction mixture was then treated with water (100ml), extracted with DCM (3 x 100ml). The organic extracts were dried (MgSO4), and evaporated. The residue was then dissolved in methanol (50ml) and -35- treated with potassium carbonate (6.366g, 46.130mmol) and stirred at rt for 15min. The reaction mixture was then treated with water (100ml), extracted with DCM (3 x 200ml). The organic extracts were dried (MgS04), evaporated and chromato graphed (0-100% ethyl acetate:Hexane) to give the product as a yellow oil (428mg, 20%). MS (ES+) m/z 235 (MH+). (k) l,l-Dimethylethyl(l-{2-hydroxy-3-[7-(methyloxy)-2-oxo-3,4-dihydro-l,8- naphthyridin-1 (2H)-yl]propyl} -4-piperidinyl)carbamate A solution of 7-(methyloxy)-l-(2-oxiranylmethyl)-3,4-dihydro-l,8-naphthyridin- 2(1H)-one (428mg, 1.829mmol) and 1,1-dimethylethyl 4-piperidinylcarbamate (366mg, 1.829mmol) in DMF (2ml) under argon was heated at 120°C for 1h. The mixture was then evaporated and chromatographed (0-10% methanol/DCM) to give the product as a yellow oil (301mg, 38%). MS (ES+) m/z 435 (MH+). (1) 1,1 -Dimethylethyl {1 -[(4,9-dioxo-1,2,8,9-tetrahydro-4H,7H-imidazo[ 1,2,3-y ]-1,8- naphthyridin-2-yl)methyl]-4-piperidinyl}carbamate A solution of 1,1-dimethylethyl (l-{2-hydroxy-3-[7-(methyloxy)-2-oxo-3,4- dihydro-l,8-naphthyridin-l(2H)-yl]propyl}-4-piperidinyl)carbamate(301mg, 0.694mmol) in chloroform (10ml) and triethylamine (0.24ml, 1.735mmoI) at rt under argon was treated with methanesulfonic anhydride (242mg, 1.388mmol) and heated at reflux for 2h. The reaction mixture was then evaporated and dissolved in acetontrile (10ml), treated with sodium iodide (520mg, 3.47mmol) and heated at 80°C for 0.25h. The mixture was then cooled, evaporated was then treated with water (200ml), extracted with 20% methanol/DCM (3 x 200ml). The organic extracts were dried (MgS04), evaporated and chromatographed (0-10% methanol/DCM) to give the product as an orange oil. (194mg, 70%). MS(ES+)m/z403(MH+). (m) 1,1 -Dimethylethyl {1 -[(4,9-dioxo-1,2-dihydro-4H,9H-imidazo[ 1,2,3-y]-1,8- naphthyridin-1 -yl)methyl]-4-piperidinyl} carbamate A solution of 1,1-dimethylethyl {l-[(4,9-dioxo-l,2,8,9-tetrahydro-4H,7Himidazo[ l,2,3-ij]-l,8-naphthyridin-l-yl)methyl]-4-piperidinyl}carbamate(194mg, 0.0.483 mmol) (301mg, 0.694mmol) in 1,4-dioxane (5ml) was treated with DDQ (164mg, 0.724mmol) and stirred at 60°C for 24h. Further DDQ (164mg, 0.724mmol) was added and the reaction was stirred for a further 2h. The reaction was then treated with 5% aqueous potassium carbonate (100ml), extracted with 20% methanol/DCM (3 x 200ml). The organic extracts were dried (MgSO4) and evaporated to give the product as an orange oil (159mg, 82%). MS (ES+) m/z 401 (MH+). (n) 1 -[(4-Amino-1 -piperidinyl)methyl]-l ,2-dihydro-4H,9H-imidazo[ 1,2,3-y]-1,8- naphthyridine-4,9-dione dihydrochloride -36- A solution of 1,1-dimethylethyl {l-[(4,9-dioxo-l,2-dihydro-4H,9H-imidazo[l,2,3- y]-l,8-naphthyridin-l-yl)methyl]-4-piperidinyl}carbamate (159mg, 0.398mmol) in chloroform (2ml) and methanol (2ml) under argon at rt was treated with 4M HC1 in 1,4- dioxane (2ml) and stirred at rt for 0.5h. The reaction was then dried and evaporated to give the product as a yellow solid (138mg, 93%). MS (ES+) m/z 301 (MH+). (o) Title compound A mixture of l-[(4-amino-l-piperidinyl)methyl]-l,2-dihydro-4H,9Himidazo[ l,2,3-zy]-l,8-naphthyridine-4,9-dione dihydrochloride (49mg, 0.131mmol) in DCM (2ml) and methanol (0.1ml) under argon at rt was treated with triethylamine (58ul, 0.419mmol) and stirred at rt for 0.25h before addition of [l,3]oxathiolo[5,4-c]pyridine-6- carbaldehyde (for a synthesis see WO2004058144 Example 61) (22mg, 0.131mmol). The mixture was then stirred at rt for 1h before addition of NaBH(OAc)3 (56mg, 0.262mmol). The reaction was stirred at rt for a further 0.5h before addition of saturated aqueous sodium bicarbonate (20ml). The mixture was extracted with 20% methanol/DCM (3 x 100ml). The organic extracts were dried (MgS04), evaporated and chromatographed (0- 20% methanol/DCM) to give the product as a clear oil (28mg, 47%). MS (ES+) m/z 452 (MH+). 8H (CDC13, 400MHz) 1.38-1.48 (2H, m), 1.78-1.95 (2H, m), 2.15-2.37 (2H, m) 2.45-2.60 (1H, m), 2.61-2.72 (2H, m), 2.92-3.02 (1H, m), 3.05-3.12 (1H, m), 3.83 (2H, s), 4.32-4.42 (1H, m), 4.52-4.61 (1H, m), 4.96-5.05 (1H, m), 5.74 (2H, s), 6.22-6.32 (2H, m), 7.20 (iH, s), 7.45-7.52 (2H, m), 7.99 (1H, s). The free base of the title compound in methanol and chloroform was converted to the hydrochloride salt by adding an equivalent of 4M hydrogen chloride in 1,4-dioxane, followed by evaporation to dryness. Example 3 1-({4-[(2,3-Dihydro[l,4]dioxino[2,3-c]pyridin-7-ylmethyl)amino]-lpiperidinyl} methyl)-l,2-dihydro-4H,9H-imidazo[l,2,3-ij]-l,8-naphthyridine-4,9- dione hydrochloride Method A A mixture of l-[(4-amino-l-piperidinyl)methyl]-l,2-dihydro-4H,9Himidazo[ l,2,3-H]-l,8-naphthyridine-4,9-dione dihydrochloride (36mg, 0.0965mmol) (for a preparation see Example 2(n) in DCM (2ml) and methanol (0.1ml) under argon at rt was treated with triethylamine (43ul, 0.309mmol) and stirred at rt for 0.25h before addition of 2,3-dihydro[l,4]dioxino[2,3-c]pyridine-7-carboxaldehyde (for a synthesis see WO2004058144 Example 2(c) or WO03/087098 Example 19(d))) (16mg, 0.0965mmol). -37- The mixture was then stirred at rt for lh before addition of NaBH(OAc)3 (41mg, 0.193mmol). The reaction was stirred at rt for a further 0.5h before addition of saturated aqueous sodium bicarbonate (20ml). The mixture was extracted with 20% methanol/DCM (3 x 100ml). The organic extracts were dried (MgSC^), evaporated and chromatographed (0-20% methanol/DCM) to give the free base of the title compound as a clear oil (24mg, 55%). MS (ES+) m/z 450 (MH+). δH (CDC13,400MHz) 1.30-1.50 (2H, m), 1.80-1.92 (2H, m), 2.19-2.35 (2H, m) 2.49-2.72 (3H, m), 2.92-3.02 (1H, m), 3.07-3.13 (1H, m), 3.81 (2H, s), 4.22-4.51 (5H, m) 4.52-4.60 (1H, m), 4.96-5.04 (1H, m), 6.22-6.32 (2H, m), 6.81 (1H, s), 7.45-7.53 (2H, m), 8.04 (1H, s). The free base of the title compound in methanol and chloroform was converted to the hydrochloride salt by adding an equivalent of 4M hydrogen chloride in 1,4-dioxane, followed by evaporation to dryness. Method B (a) 2-Bromo-3-[(phenylmethyl)oxy]propanoic acid Racemic O-(phenylmethyl)serine (5g, 25.6 mmol) and potassium bromide (10.7g, 89.6mmol) were dissolved in ice-cooled H2SO4 (2.5N) and treated with an solution of sodium nitrite (2.65g) in water (30ml) over 50 minutes (keeping the reaction temperature lh, extracted with ethyl acetate (3 x 100ml). The combined organic extracts were washed with water, brine, dried (MgSO4), filtered and evaporated to give the product as a yellow oil (6g, 90%). MS (ES+) m/z 259/261 (MH+). (b) Methyl 2-bromo-3-[(phenylmethyl)oxy]propanoate A solution of 2-bromo-3-[(phenylmethyl)oxy]propanoic acid (6g, 23.2mmol) in methanol (40ml) at rt under argon was treated with thionyl chloride (1.7ml, 23.2mmol) and the reaction was then stirred at rt for 3h and then evaporated to give product as a yellow oil (6.3g, 99%). MS (ES+) m/z 273/275 (MH+). (c) Methyl 2-[4-((2,3-dihydro[l,4]dioxino[2,3-c]pyridin-7-ylmethyl){[(l,ldimethylethyl) oxy]carbonyl) amino)-1 -piperidinyl]-3-[(phenylmethyl)oxy]propanoate A mixture of 1,1-dimethylethyl (2,3-dihydro[l,4]dioxino[2,3-c]pyridin-7- ylmethyl)4-piperidinylcarbamate (1.087 g, 3.11 mmol) (for a synthesis see WO2004/058144 Example 99(h)), methyl 2-bromo-3-[(phenylmethyl)oxy]propanoate (1.0 g, 3.66 mmol) and potassium carbonate (0.860 g, 6.22 mmol) in DMF (50 ml) was heated to 80°C and stirred under argon for 2.5h. The solvents were removed under reduced pressure and the residue treated with saturated aqueous sodium bicarbonate. The aqueous was extracted with DCM (5 x 100ml) dried MgSO4, filtered and concentrated under reduced pressure. The crude product was chromatographed, eluting with 0-100% -38- EtOAc/40-60 petroleum ether. Appropriate fractions were combined and evaporated under reduced pressure. The residue was then dissolved in DCM (50ml) and washed with water (20ml). The organic layer was separated, dried MgSO and evaporated under reduced pressure to afford product (618mg, 35% yield). MS (ES+) m/z 542 (MH+). (d) l,l-Dimethylethyl(2,3-dihydro[l,4]dioxino[2,3-c]pyridin-7-ylmethyl)[l-(2-hydroxy- 1 - {[(phenylmethyl)oxyjmethyl} ethyl)-4-piperidinyl] carbamate A solution of methyl 2-[4-((2,3-dihydro[l,4]dioxino[2,3-c]pyridin-7- ylmethyl) {[(1,1 -dimethyl ethyl)oxy] carbonyl} amino)-1 -piperidinyl] -3 - [(phenylmethyl)oxy]propanoate (618mg, l.Hlmmol) in dry THF (8 ml) at -78 °C under Ar was added LiAJH4 (1.312 ml, 1.312 mmol) dropwise. The reaction mixture was allowed to warm to ~-10°C over 2h. The mixture was then stirred at 0°C for 2h before addition of water (0.1ml), then sodium hydroxide (0.18 ml, 0.360 mmol) and then water (0.2ml). The mixture was then stirred for a further 2h at it. The resulting mixture was filtered and washed with THF (100ml). The combined filtrate and washings were evaporated under reduced pressure to afford the product (0.519g, 89% yield). MS(ES+)m/z514(MH+). (e) 1,1 -Dimethylethyl [ 1 -(2-chloro-1 - {[(phenylmethyl)oxy]methyl} ethyl)-4- piperidinyl](2,3-dihydro[l,4]dioxino[2,3-c]pyridin-7-ylmethyl)carbamate A solution of 1,1-dimethylethyl (2,3-dihydro[l,4]dioxino[2,3-c]pyridin-7- ylmethyl)[ 1 -(2-hydroxy-1- {[(phenylmethyl)oxyjmethyl} ethyl)-4-piperidinyl]carbamate (150 mg, 0.292 mmol) and triethylamine (0.049 ml, 0.350 mmol), in DCM (5 ml) at 0 °C was treated with methanesulfonyl chloride (0.025 ml, 0.321 mmol). The solution was allowed to warm to room temperature and stirred at this temperature for lh. A further 0.2eq of triethylamine and 0.4eq of methanesulfonyl chloride was added and the reaction stirred for 30 mins. The reaction mixture was diluted with DCM (20ml) and treated with water (2ml). The aqueous layer was extracted again with DCM (50ml). The organic layers were combined and dried MgS04, filtered and evaporated under reduced pressure to the crude product (lOlmg, 65%), which was used without further purification. MS (ES+) m/z 532/534 (MH+). (f)l,l-Dimethylethyl(2,3-dihydro[l,4]dioxino[2,3-c]pyridin-7-ylmethyl)[l-(2-[7- (methyloxy)-2-oxo-3,4-dihydro-1,8-naphthyridin-1 (2H)-yl]-l - {[(phenylmethyl)oxy]methyl} ethyl)-4-piperidinyl]carbamate Method 1: A solution of 1,1-dimethylethyl [1 -(2-chloro-1- {[(phenylmethyl)oxy]methyl}ethyl)-4-piperidinyl](2,3-dihydro[l,4]dioxino[2,3- c]pyridin-7-ylmethyl)carbamate (lOlmg, 0.190 mmol) in DMF (10 ml) was added dropwise to a solution of the sodium salt of 7-(methyloxy)-3,4-dihydro-l,8-naphthyridin- 2(lH)-one (33.8 mg, 0.190 mmol) in DMF) (10 ml) (prepared from addition of sodium hydride (9.11 mg, 0.228 mmol) to 7-(methyloxy)-3,4-dihydro-l,8-naphthyridin-2(lH)- one (33.8 mg, 0.190 mmol) (for a preparation see Example 5(e)) in DMF( 10ml)).The -39- solution was stirred at room temperature overnight under Ar. The reaction was then heated to 60°C and stirred at this temperature under Ar for lh. The reaction was cooled to rt and a further eq of sodium hydride (9.11 mg, 0.228 mmol) was added with stirring under argon. The reaction was stirred at rt for 72h. Method 2: A solution of 1,1-dimethylethyl [l-(2-chloro-l- {[(phenylmethyl)oxy]methyl} ethyl)-4-piperidinyl](2,3-dihydro[ 1,4]dioxino[2,3- c]pyridin-7-ylmethyl)carbamate (343 mg, 0.645 mmol) in DMF (10 ml) was added dropwise to a solution of the sodium salt of 7-(methyloxy)-3,4-dihydro-l,8-naphthyridin- 2(lH)-one (138 mg, 0.774 mmol) (prepared from the addition of sodium hydride (60%, 38.7 mg, 0.967 mmol) to 7-(methyloxy)-3,4-dihydro-l,8-naphthyridin-2(lH)-one (138 mg, 0.774 mmol) (for a preparation see Example 5(e)) in DMF (10ml)). The solution was stirred at room temperature overnight under argon. The reaction mixtures from Method 1 and Method 2 were combined and the DMF was removed under reduced pressure. The residue was treated with saturated aqueous sodium bicarbonate solution (10ml) and water (20ml) and extracted with DCM (3 x 100ml). The combined organic layers were dried (MgSO ), filtered and removed under reduced pressure. The crude product was chromatographed, eluting with 0-100% EtOAc/hexane. Appropriate fractions were combined to give two batches of product (batchl: 167mg, 38%) and (batch2: lower purity, 78mg, 18%). MS (ES+) m/z 674 (MH+). (g) 1,1 -Dimethylefhyl (2,3-dihydro[ 1,4]dioxino[2,3-c]pyridm-7-ylmefhyl)[l -(2-hydroxy- 1 - {[7-(methyloxy)-2-oxo-3,4-dihydro-1,8-naphthyridin-1 (2H)-yl]methyl} ethyl)-4- piperidinyl]carbamate A solution of 1,1-dimethylethyl (2,3-dihydro[l,4]dioxino[2,3-c]pyridin-7- ylmethyl)[l-(2-[7-(methyloxy)-2-oxo-3,4-dihydro-l,8-naphthyridin-l(2H)-yl]-1- {[(phenylmethyl)oxy]methyl}ethyl)-4-piperidinyl]carbamate (167 mg, 0.248 mmol) in ethanol (20 ml) was hydrogenated at 1 atmosphere hydrogen pressure for approximately 9 days. The reaction was filtered through Celite and washed with ethanol. The combined filtrate and washings were evaporated under reduced pressure to afford the product (162 mg,91%). MS (ES+) m/z 584 (MH+). (h) 1,1 -Dimethylethyl (2,3-dihydro[ 1,4]dioxino[2,3-c]pyridin-7-ylmethyl) {1 -[(4,9-dioxo- 1,2,8,9-tetrahydro-4H,7H-imidazo[ 1,2,3-ij]-l ,8-naphthyridin-1 -yl)methyl]-4- piperidinyl} carbamate A solution of 1,1-dimethylethyl (2,3-dihydro[l,4]dioxino[2,3-c]pyridin-7- ylmethyl)[ 1 -(2-hydroxy-1 - {[7-(methyloxy)-2-oxo-3,4-dihydro-1,8-naphthyridin-1 (2H)- yl]methyl}ethyl)-4-piperidinyl]carbamate (162mg, 0.278 mmol) in DCM (10ml) under argon was cooled to 0 °C and treated with triethylamine (0.046 ml, 0.333 mmol) and methanesulfonyl chloride (0.026 ml, 0.333 mmol). The reaction was allowed to warm to rt and stirred at this temperature for lh. A further 1.2 eq of triethylamine (0.046 ml, 0.333 mmol) and methanesulfonyl chloride (0.026 ml, 0.333 mmol) were added and the -40- solution stirred at rt overnight. A further 1.2eq of triethylamine (0.046 ml, 0.333 mmol) and methanesulfonyl chloride (0.026 ml, 0.333 mmol) were added and the solution heated to 50°C for 6h. The solution was cooled to rt, saturated aqueous NaHCO (10ml) was added and the aqueous extracted with 20%MeOH/DCM (3 x 100ml). The organic phases were combined, dried MgS04, filtered and concentrated. The crude product was then chromatographed, eluting with 0-15% MeOH/DCM. Appropriate fractions were combined and evaporated under reduced pressure to afford the product (80 mg, 48%). MS(ES+)m/z552(MH+). (i) 1,1 -Dimethylethyl (2,3-dihydro[l,4]dioxino[2,3-c]pyridin-7-ylmethyl){1 -[(4,9-dioxol, 2-dihydro-4H,9H-imidazo[l,2,3-y]-l,8-naphthyridin-l-yl)methyl]-4- piperidinyl} carbamate A solution of 1,1-dimethylethyl (2,3-dihydro[l,4]dioxino[2,3-c]pyridin-7- ylmethyl){l-[(4,9-dioxo-l,2,8,9-tetrahydro-4H,7H-imidazo[l,2,3-ij]-l,8-naphthyridin-lyl) methyl]-4-piperidinyl}carbamate (80 mg, 0.145 mmol) and DDQ (49.4 mg, 0.218 mmol) in 1,4-dioxane (5 ml) was stirred at 120°C for 2h. A further 0.5 eq of DDQ (17mg) was added and the solution stirred for a further 2h. The mixture was allowed to cool to rt and was treated with sat NaHCO3 (10ml). The aqueous layer was extracted with 20%MeOH/DCM (3 x 100ml). The organic layers were combined, dried MgS04, filtered and concentrated to afford the crude product (64mg, 83%). MS (ES+) m/z 550 (MH+). (j) Title compound A solution of 1,1-dimethylethyl (2,3-dihydro[l,4]dioxino[2,3-c]pyridin-7- ylmethyl) {1 -[(4,9-dioxo-l ,2-dihydro-4H,9H-imidazo[ 1,2,3-ij]-l,8-naphthyridin-1 - yl)methyl]-4-piperidinyl} carbamate (64 mg, 0.116 mmol) in DCM (2 ml) and HC1 in 1,4- dioxane (0.291 ml, 1.164 mmol) was stirred at rt for 2h. The solvents were removed under reduced pressure. The crude product was added to an ion exchange column and was eluted with MeOH (20ml) and then 2M NH in MeOH (15ml) to give the free base of the title compound (34mg, 65%). 1H NMR and LC-MS identical to product of Example 3 A. The free base of the title product was then converted into the HC1 salt by dissolving in DCM (2ml) and treating with leq 1M HC1 in ether. Solvents were removed under reduced pressure to afford the title hydrochloride salt. Example 4 l-({4-[(2,3-Dihydro[l,4]dioxino[23-c]pyridin-7-ylmethyl)amino]-lpiperidinyl} methyl)-l,2-dihydro-4H,9H-imidazo[l,2,3-ij]-l,8-naphthyridine-4,9- dione hydrochloride 1 -41- A mixture of l-[(4-amino-l-piperidinyl)methyl]-l,2-dihydro-4H,9Himidazo[ l,2,3-y]-l,8-naphthyridine-4,9-dione dihydrochloride (51mg, 0.136mmol) (for a preparation see Example 2(n)) in DCM (2ml) and methanol (0.1ml) under argon at rt was treated with triethylamine (60ul, 0.438mmol) and stirred at rt for 0.25h before addition of 7-bromo-3-oxo-3,4-dihydro-2H-pyrido[3,2-6][l ,4]thiazine-6-carboxaldehyde (for a synthesis, see WO 2002056882 Example 33(e)) (37mg, 0.136mmol). The mixture was then stirred at rt for lh before addition of NaBH(OAc)3 (86mg, 0.408mmol). The reaction was stirred at rt for a further 0.5h before addition of saturated aqueous sodium bicarbonate (20ml). The mixture was extracted with 20% methanol/DCM (3 x 100ml). The organic extracts were dried (MgSCv), evaporated and chromatographed (0-20% methanol/DCM) to give the free base of the title compound as a clear oil (36mg, 48%). MS(ES+)m/z558(MH+). δH (CDC13, 400MHz) 1.32-1.51 (2H, m), 1.81-2.00 (2H, m), 2.20-2.41 (2H, m) 2.50-2.75 (3H, m), 2.93-3.03 (1H, m), 3.04-3.15 (1H, m), 3.46 (2H, s), 3.98 (2H, s), 4.32-4.41 (1H, m) 4.52-4.61 (1H, m), 4.98-5.04 (1H, m), 6.22-6.32 (2H, m), 7.48-7.51 (2H, m), 7.75 (lH,s). The free base in methanol and chloroform was converted to the title hydrochloride salt by adding an equivalent of 4M hydrogen chloride in 1,4-dioxane, followed by evaporation to dryness. Example 5A (1R)-1-({4-[([1,3]OxathioIo[5,4-clpyridin-6-ylmethyI)amino]-lpiperidinyl} methyl)-l,2-dihydro-4H,9H-imida2o[l,2,3-H]-l,8-naphthyri(line-4,9- dione hydrochloride (a)2,2-Dimethyl-N6-(methyloxy)-2-pyridinyl]propanamide A suspension of trimethylacetamide (18.08 g, 178.744 mmol), Cs2CO3 (68.823g, 211.242 mmol), Pd2(dba)3 (1.488g, 1.625 mmol) and Xantphos (4,5-bis- (diphenylphosphino)-9,9-dimethylxanthene)(1.880g, 3.249 mmol) in dry, degassed 1,4- dioxane (800ml) under argon was sonicated for 0.25h and then treated with 2-chloro-6- (methyloxy)pyridine (19.32 ml, 162.494 mmol). The mixture was then heated at reflux for 24h. The mixture was evaporated, treated with water (1L) and extracted 3x DCM (1L and then 2x 500ml). The organic extracts were dried (MgSO4), evaporated and -42- chromatographed (50-100% DCM/40-60 Petroleum ether then 0-5% methanol/DCM) to give title compound as a yellow solid (25.191g, 121.111 mmol, 75%). Impure fractions were recolumed (eluting as above) to give more product (4.990g, 23.990 mmol, 15%). Total yield of 90%. MS (ES+) m/z 209 (MH+, 100%). (b) N-[3-Bromo-6-(methyloxy)-2-pyridinyl]-2,2-dimethylpropanamide A solution of 2,2-dimethyl-N-[6-(methyloxy)-2-pyridinyl]propanamide (55.01 lg, 264.467 mmol) in THF (450 ml) in a three necked 1L flask with an internal thermometer under argon was cooled to -78 °C and treated with n-butyl lithium (232 ml, 581.847 mmol) over 15 minutes and then allowed to warm to 0°C and stirred at 0°C for 7h. The mixture was then recooled to -78°C and treated with 1,2-dibromoethane (27.3 ml, 317 mmol) over 10 minutes and then the solution was allowed warm to room temperature and stirred at room temperature for 30 minutes by which time all the solid which had formed dissolved again. Gas was evolved at this stage so a gas bubbler was placed on one of the flasks necks. Water (100ml) was then carefully added over 10 minutes. Further water (500ml) was then added and the mixture was extracted with diethyl ether (3 x 500ml). The combined organic solvents were then dried (MgSO ), filtered, evaporated to give the crude product. This was then dissolved in warm ethyl acetate (100ml) and allowed to stand in the freezer overnight. The resultant solid which crystallised out was filtered off, washed with ice-cooled diethyl ether (20ml) and dried in vacuo to give product as a white solid (45.660g, 159.01 lmmol, 60% yield). The filtrate was evaporated and the residue was chromatographed (0-25% ethyl acetate/40-60 petroleum ether) to give recovered starting material (7.264g, 34.9mmol), and product as a white solid (8.038g, 27.992mmol, 10% yield). The product from recrystallisation and silica chromatography were identical by NMR and LC-MS and so were combined. MS (ES+) m/z 287/289 (MH+, 100%). (c) Butyl (2E)-3 -[2-[(2,2-dimethylpropanoyl)amino]-6-(methyloxy)-3 -pyridinyl]-2- propenoate A mixture of N-[3-bromo-6-(methyloxy)-2-pyridinyl]-2,2-dimethylpropanamide (78.783g, 274mmol), bis(tri-t-butylphosphine)palladium(0) (l g, 1.957 mmol) and tris(dibenzylideneacetone)dipalladium(0) (0.892 g, 0.974 mmol) in dry, degassed 1,4- dioxane (600 ml) was treated with n-butyl acrylate (47.1 ml, 329 mmol) and dicyclohexylmethylamine (64.5 ml, 302 mmol). The reaction mixture was then heated at 80 °C for 4h and then at 120 °C for 3h. The reaction was then evaporated and water (1000ml) was added and the mixture was extracted with diethyl ether (3 x 500ml). The combined organic solvents were then dried (MgSO4), filtered, evaporated to give the crude product. This was then dissolved in DCM (300 ml) and chromatographed (10-30% ethyl acetate :40-60 petroleum ether) and then dried in vacuo to give product as a white solid (87.412g, 95%). MS (ES+) m/z 335 (MH+, 100%). -43- (d) Butyl 3-[2-[(2,2-dimethylpropanoyl)amino]-6-(methyloxy)-3-pyridinyl]propanoate A solution of butyl (2E)-3-[2-[(2,2-dimethylpropanoyl)amino]-6-(methyloxy)-3- pyridinyl]-2-propenoate (43.706 g, 131 mmol) in ethanol (450 ml) under argon at rt was treated with palladium on carbon (5.0 g, 47.0 mmol) and then stirred at rt under 1 atmosphere of hydrogen for 90h. The reaction mixture was then filtered through a thin pad of Kieselguhr, washing the product through with further ethanol (200ml). The solvent was then evaporated to give product as a yellow oil (43.549, 99%). MS (ES+) m/z 337 (MH+, 100%). (e) 7-(Methyloxy)-3,4-dihydro-l ,8-naphthyridin-2(1H)-one A mixture of butyl 3-[2-[(2,2-dimethylpropanoyl)amino]-6-(methyloxy)-3- pyridinyl]propanoate (86.01 g, 256 mmol) in hydrochloric acid (500 ml, 3000 mmol)(6M aqueous), was heated at 80 °C for 6h. Reaction was cooled, treated with water (500ml), transferred to a 5L conical flask and carefully neutralised with solid potassium carbonate (requires around 250g)(much effervescence was observed). The mixture was then extracted with 20% MeOH/DCM (3 x 500ml). The combined organic solvents were then dried (MgSO ), filtered and evaporated to give the crude product as a yellow solid (35.84g, 79%). MS (ES+) m/z 179 (MH+, 100%). (f)7-(Methyloxy)-l-[(2R)-2-oxiranylmethyl]-3,4-dihydro-l,8-naphthyridin-2(li[/)-one A solution of 7-(methyloxy)-3,4-dihydro-l,8-naphthyridin-2(lH)-one (4.974 g, 27.9 mmol) in DMF (100 ml) at 0°C under argon was treated with sodium hydride (60%, 1.340 g, 33.5 mmol) and allowed to stir at 0°C for 20min. The reaction mixture was then treated with (2,S)-2-oxiranylmethyl 3-nitrobenzenesulfonate (7.60 g, 29.3 mmol), stirred at 0°C and then allowed warm to rt and stirred at rt for lh. Water (5ml) was then added. Reaction was evaporated, saturated aqueous bicarbonate (500ml) was then added and the mixture was extracted with DCM (3 x 500ml). The combined organic solvents were then dried (MgSO ), filtered and evaporated to give the crude product. MS (ES+) m/z 235 (MH+, 100%). (g)(15)-l-(Hydroxymethyl)-l,2,5,6-tetrahydro-4H,9H-imidazo[l,2,3-ij]-1,8- naphthyridine~4,9-dione A solution of 7-(methyloxy)-l-[(2R)-2-oxiranylmethyl]-3,4-dihydro-l,8- naphthyridin-2(lH)-one (1.167 g, 4.98 mmol) in DMF (20 ml) under argon was heated to 120°C for 6h. Reaction was then evaporated and chromatographed (0-20% methanol/DCM) to give product as an orange solid (339mg, 31%). MS (ES+) m/z 221(MH+, 100%). Alternatively the reaction can be heated with microwave power at 160°C for 40mins. (h) 1,1 -Dimethylethyl (1 - {[(2H)-4,9-dioxo-1,2,8,9-tetrahydro-4H,7H-imidazo[ 1,2,3-ij]- 1,8-naphthyridin-2-yl]methyl} -4-piperidinyl)carbamate -44- A solution of (1 S)-l-(hydroxymethyl)-1,2,5,6-tetrahydro-4H,9H-imidazo[l ,2,3- ij]-l,8-naphthyridine-4,9-dione (1.909 g, 8.67 mmol) in DCM (100 ml) at 0°C under argon was treated with triethylamine (1.450 ml, 10.40 mmol) and then methanesulfonyl chloride (0.743 ml, 9.54 mmol) and then allowed to warm to rt and stirred at it for lh. The reaction mixture was then treated with saturated aqueous bicarbonate (100ml) and the mixture was extracted with DCM (2 x 100ml). The combined organic solvents were then dried (MgSO4), filtered and evaporated to give the crude intermediate [(2S)-4,9- dioxo-l,2,8,9-tetrahydro-4H,7H-imidazo[l,2,3-y]-l,8-naphthyridin-2-yl]methyl methanesulfonate. This was dissolved in dry acetonitrile (100 ml) and then treated with pyridine (1.402 ml, 17.34 mmol) and 1,1-dimethylethyI 4-piperidinylcarbamate (3.47 g, 17.34 mmol) and heated at 70°C for 20h. After 20h more 1,1-dimethylethyl 4- piperidinylcarbamate (3.47 g, 17.34 mmol) and pyridine (1.402 ml, 17.34 mmol) were added and the temperature was increased to reflux (heating block 95°C) and reaction was stirred at this temperature for a further 4h. The reaction mixture was then evaporated, saturated aqeous NaHCO (200ml) was then added and the mixture was extracted with DCM (3 x 200ml). The combined organic solvents were then dried (MgSO4), filtered and evaporated to give the crude product as a brown solid. MS (ES+) m/z 403(MH+, 100%). (i) 1,1 -Dimethylethyl (1 -{[(1R)-4,9-dioxo-1,2-dihydro-4H,9H-imidazo[ 1,2,3-ij]-1,8- naphthyridin-1 -yl]methyl} -4-piperidinyl)carbamate A solution of 1,1-dimethylethyl (l-{[(2R)-4,9-dioxo-l,2,8,9-tetrahydro-4H,7Himidazo[ l,2,3-ij]-l,8-naphthyridin-2-yl]methyl}-4-piperidinyl)carbamate (5.710 g, 14.19 mmol) in 1,4-dioxane (50 ml) at rt was treated with DDQ (4.83 g, 21.28 mmol) and then heated at 120°C for lh. The reaction was then cooled to rt. The reaction mixture was treated with saturated aqueous KCO (5%, 1000ml) and extracted with DCM (3 x 500ml). The combined organic solvents were then dried (MgSO4), filtered and evaporated to give the crude product as a brown solid. The reaction was repeated using a further portion of carbamate (2.889 g, 7.18 mmol) in 1,4-dioxane (50 ml) with DDQ (2.444 g, 10.77 mmol). The reaction was performed and worked up as above and the combined residues were chromatographed (0-100% ethyl acetate:40-60 Petroleum ether then 0-20% methanol:ethyl acetate) to give the product as a brown solid (1.532g). MS (ES+) m/z 401(MH+, 100%). (j)(1R)-l-[(4-Amino-l-piperidinyl)methyl]-l,2-dihydro-4H,9H-imidazo[l,2,3-ij']-1,8- naphthyridine-4,9-dione dihydrochloride A solution of 1,1-dimethylethyl (l-{[(1R)-4,9-dioxo-l,2-dihydro-4H,9Himidazo[ l,2,3-ij]-l,8-naphthyridin-l-yl]methyl}-4-piperidinyl)carbamate (1.532 g, 3.83 mmol) in chloroform (20 ml) under argon at rt was treated with 4M HC1 in 1,4-dioxane (10 ml, 40.0 mmol) and stirred at rt for 0.25h. Methanol (20ml) was then added and reaction was stirred for a further 0.25h. The reaction was then evaporated and triturated with diethyl ether (20ml). The solid was then dried in vacuo to give the impure product as a brown solid (1.443g, 101%). -45- MS (ES+) m/z 301(MH+, 100%) 1 -[(4-Amino-1 -piperidinyl)methyl]-l ,2-dihydro-4H,9H-imidazo[ 1,2,3-ij]-l ,8- naphthyridine-4,9-dione dihydrochloride made by this general method (Example 5(a)-(j)) was analyzed via chiral HPLC (Chiralpak AS-H (5 microns) and found to be a single enantiomer, presumed to be R. (k) Title compound A suspension of (1R)-1 -[(4-amino-1 -piperidinyl)methyl]-1,2-dihydro-4H,9Himidazo[ l,2,3-ij]-l,8-naphthyridine-4,9-dione dihydrochloride (impure product) (575 mg, 1.540 mmol) in chloroform (20 ml) and methanol (1 ml) at rt under argon was treated with triethylamine (0.644 ml, 4.62 mmol) and stirred at rt for 0.25h. The solution was then treated with [l,3]oxathiolo[5,4-c]pyridine-6-carbaldehyde (for a synthesis see WO2004058144 Example 61) (258 mg, 1.540 mmol) and stirred for a further 0.5h. The solution was then treated with NaBH(OAc)3 (979 mg, 4.62 mmol) and stirred at rt for 0.5h. The reaction was then treated with saturated aqueous NaHCO (100ml) and extracted with 20% methanol/DCM (3 x 200ml). The combined organic extracts were dried (MgSO ), filtered, evaporated and chromatographed (0-20% methanol/DCM) to give the free base of the title compound as a light brown solid (574 mg, 1.273 mmol, 83%). δH (CDC13, 250MHz) 1.25-1.45 (2H, m), 1.75-1.95 (2H, m), 2.20-2.45 (2H, m), 2.45- 2.55 (1H, m), 2.60-2.75 (2H, m), 2.90-3.00 (1H, m), 3.05-3.15 (1H, dd), 3.85 (2H, s), 4.30-4.40 (1H, m), 4.55-4.65 (1H, m), 4.95-5.05 (1H, m), 5.75 (2H, s), 6.25 (1H, m), 6.30 (1H, m), 7.20 (1H, s), 7.45-7.52 (2H, m), 8.00 (1H, s) MS(ES+)m/z452(MH+). The free base in DCM/MeOH 2:1 (15ml) was treated with 1M HC1 in diethyl ether and then evaporated to give the title monohydrochloride salt. Example 5B (1R)-l-({4-[([l,3]Oxathiolo[5,4-c]pyrid[in-6-ylmethyl)amino]-lpiperidinyl} methyl)-l,2-dihydro-4H,9H-imidazo[l,2,3-ij]-l,8-naphthyridine-4,9- dione benzoate (1R)-l-({4-[([l,3]Oxathiolo[5,4-c]pyridin-6-ylmethyl)amino]-lpiperidinyl} methyl)-l ,2-dihydro-4H,9H-imidazo[ 1,2,3-H]-1,8-naphthyridine-4,9-dione was dissolved in methanol and treated with benzoic acid (1 equivalent). Concentration, treatment with diethyl ether and evaporation of the solvents under reduced pressure gave the product as the benzoate salt. Example 5C (1R)-l-({4-[([l,3]Oxathiolo[5,4-c]pyridin-6-ylmethyl)amino]-lpiperidinyl} methyl)-1,2-dihydro-4H,9H-imidazo[l,2,3-ij]-l,8-naphthyridine-4,9- dionedi-trifluoroacetate(lR)-l-({4-[([l,3]Oxathiolo[5,4-c]pyridin-6-ylmethyl)amino]- l-piperidinyl}methyl)-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione hydrochloride in eluent [10% MeCN in water (containing 0.1% TFA)] was applied to a preparative reverse phase HPLC column. Product-containing fractions were combined, -46- concentrated and concentrate lyophilized. The product was isolated as a sticky white foam following desiccation (weekend) over P2O5. Example 6A (1R)-l-({4-[(2,3-Dihydro[l,4]oxathiino[23-clpyridin-7- ylmethyl)aminol-1-piperidinyl}methyl)-1,2-dihydro-4H,9H-imidazo[1,2,3-ij]-1,8- naphthyridine-4,9-dione hydrochloride A suspension of (1R)-l-[(4-amino-l-piperidinyl)methyl]-l,2-dihydro-4H,9Himidazo[ l,2,3-ij]-l,8-naphthyridine-4,9-dione dihydrochloride (511 mg, 1.369 mmol) (for a preparation see Example 5(j)) in chloroform (20 ml) and methanol (1 ml) at rt under argon was treated with triethylamine (0.572 ml, 4.11 mmol) and stirred at rt for 0.25h. The solution was then treated with 2,3-dihydro[l,4]oxathiino[2,3-c]pyridine-7- carbaldehyde (for a synthesis see WO2004058144, Example 60) (248 mg, 1.369 mmol) and stirred for a further 0.5h. The solution was then treated with NaBH(OAc)3 (870 mg, 4.11 mmol) and stirred at rt for 0.5h. The reaction was then treated with saturated aqueous NaHC03 (100ml) and extracted with 20% methanol/DCM (3 x 200ml). The combined organic extracts were dried (MgSO ), filtered, evaporated and chromatographed (0-20% methanol/DCM) to give the free base of the title compound as a light brown solid (499mg, 78%). MS (ES+) m/z 466 (MH+). 5H (CDCI3, 250MHz) 1.21-1.48 (2H, m), 1.72-1.92 (2H, m), 2.12-2.39 (2H, m) 2.41-2.78 (3H, m), 2.89-3.22 (4H, m), 3.78 (2H, s), 4.28-4.48 (3H, m) 4.50-4.61 (1H, m), 4.96-5.04 (1H, m), 6.19-6.32 (2H, m), 7.01 (1H, s), 7.42-7.53 (2H, m), 8.00 (1H, s). The free base in DCM/MeOH 2:1 (15ml) was treated with one equivalent of 1M HC1 in diethyl ether and then evaporated to give the title monohydrochloride salt. Example 6B (1H)-l-({4-[(2,3-Dihydro[l,4]oxathiino[2,3-c]pyridin-7- ylmethyl)aminol-1-piperidinyl}methyl)-1,2-dihydro-4H,9H-imidazo[1,2,3-ij]-1,8- naphthyridine-4,9-dione hydrochloride (1R)-l-({4-[(2,3-Dihydro[l,4]oxathiino[2,3- c]pyridin-7-ylmethyl)amino]-1 -piperidinyl} methyl)-1,2-dihydro-4H,9H-imidazo[ 1,2,3- ij]-1,8-naphthyridine-4,9-dione was dissolved in methanol and treated with benzoic acid (1 equivalent). Evaporation of the solvents under reduced pressure gave the product as the benzoate salt. Example 6C (1R)-1-({4-[(2,3-Dihydro[l,4]oxathiino[2,3-c]pyridin-7- ylmethyl)amino]-l-piperidinyl}methyl)-l,2-dihydro-4H,9H-imidazo[1,2,3-H]-1,8- naphthyridine-4,9-dione ditrifluoroacetate(1R)-1-({4-[([l,3]Oxathiolo[5,4-c]pyridin- 6-ylmethyl)amino]-l-piperidinyl}methyl)-l,2-dihydro-3H,8H-2a,5,8a- -47- triazaacenaphthylene-3,8-dione hydrochloride was applied to a preparative reverse phase HPLC column in a mixture of 10% MeCN in water containing 0.1 % TFA. Productcontaining fractions were combined, concentrated and concentrate lyophilized. The product (bis-TFA salt) was isolated as a white solid following desiccation over P2O5. Example 7 (1R)-l-({4-[(5,6,7,8-Tetrahydro-3-isoquinolinylmethyl)amino]-1- piperidinyl}methyl)-l,2-dihydro-4H,9H-imidazo[l,2,3-ij]-1,8-naphthyridine-4,9- dione hydrochloride (a) Ethyl 5,6,7,8-tetrahydro-3-isoquinolinecarboxylate A solution of 1,7-octadiyne (4.00 ml, 30.1 mmol) and ethyl cyanoformate (2.95 ml, 30.1 mmol) in dry degassed 1,4-dioxane (500 ml) under argon at rt was treated with cyclopentadienyl-Cobalt(I)-dicarbonyl (0.814 g, 4.52 mmol) and then heated at reflux for 18h. Reaction was then evaporated, treated with toluene (100ml), re-evaporated, dissolved in DCM (100ml), filtered through a short pad of Kieselguhr, eluting with DCM, organic extracts evaporated, chromatographed (0-100% DCM:40-60 Petroleum ether then 0-10% methanol/DCM) to give product as an impure brown oil (1.27g, 21 %). MS (ES+) m/z 206 (MH+). (b)5,6,7,8-Tetrahydro-3-isoquinolinylmethanol A solution of ethyl 5,6,7,8-tetrahydro-3-isoquinolinecarboxylate (1.27 g, 6.19 mmol) in THF (50 ml) at -78°C under argon was treated with LiA1H4 (1M solution in THF, 6.19 ml, 6.19 mmol) and allowed warm to rt. After 10 min at rt, water (1ml), 2M aq NaOH (1ml) and water (1ml) were sequentially added and the mixture stirred at rt for 0.5h. The mixture was then filtered through a short pad of Kieselguhr, eluting with THF (50ml), organic extracts were then evaporated, chromatographed (0-20% methanol/DCM) to give product as an orange oil (0.572g, 57%). MS(ES+)m/z 164(MH+). (c) 5,6,7,8-Tetrahydro-3-isoquinolinecarbaldehyde A solution of 5,6,7,8-tetrahydro-3-isoquinolinylmethanol (572 mg, 3.50 mmol) in (DCM) (10 ml) at rt under argon was treated with manganese dioxide (3.047 g, 35.0 mmol) and then stirred at rt for 2h. The reaction mixture was then filtered through a thin pad of Kieselguhr, eluting with DCM (50ml), the organic extracts were evaporated to give the crude product as a brown oil (435mg, 77%). MS(ES+)m/z 162(MH+). (d) Title compound -48- A suspension of (1R)-l-[(4-amino-1-piperidinyl)methyl]-l,2-dihydro-4H,9Himidazo[ l,2,3-ij]-l,8-naphthyridine-4,9-dione dihydrochloride (87 mg, 0.233 mmol) (for a preparation see Example 5(j)) in chloroform (5 ml) and methanol (0.2 ml) at rt under argon was treated with triethylamine (97 µl, 0.699 mmol) and stirred at rt for 0.25h. The solution was then treated with 5,6,7,8-tetrahydro-3-isoquinolinecarbaldehyde (37.6 mg, 0.233 mmol) and stirred for a further 0.5h. The solution was then treated with NaBH(OAc)3 (148 mg, 0.699 mmol) and stirred at rt for 0.5h. The reaction was then treated with saturated aqueous NaHCO (100ml) and extracted with 20% methanol/DCM (3 x 200ml). The combined organic extracts were dried (MgS04), filtered, evaporated and chromatographed (0-20% methanol/DCM) to give the free base of the title compound as a light brown solid (66 mg, 64%). MS (ES+) m/z 446 (MH+). δH (CDC13, 250MHz) 1.22-1.51 (2H, m), 1.71-1.99 (7H, m), 2.15-2.38 (2H, m) 2.45-2.82 (4H, m), 2.61-3.22 (4H, m), 3.85 (2H, s), 4.29-4.42 (1H, m) 4.50-4.61 (1H, m), 4.96-5.04 (1H, m), 6.18-6.32 (2H, m), 7.00 (1H, s), 7.47-7.59 (2H, m), 8.21 (1H, s). The free base in DCM/MeOH 2:1 (15ml) was treated with one equivalent of 1M HC1 in diethyl ether and then evaporated to give the title monohydrochloride salt. Example 8 (1R)-1-({4-[(6,7-Dihydro-5H-cyclopenta[c]pyridin-3-ylmethyl)amino]- l-piperidinyl}methyl)-1,2-dihydro-4H,9H-imidazo[1,2,3-ij]-1,8-naphthyridine-4,9- dione hydrochloride r^O-fi-Tf5^ H n^j? (a) Ethyl 6,7-dihydro-5H-cyclopenta[c]pyridine-3-carboxylate A solution of 1,6-heptadiyne (1.242 ml, 10.85 mmol) and ethyl cyanoformate (1.063 ml, 10.85 mmol) in dry degassed 1,4-dioxane (100 ml) under argon at rt was treated with cyclopentadienyl-Cobalt(I)-dicarbonyl (0.293 g, 1.628 mmol) and then heated at reflux for 18h. Reaction was then evaporated, treated with toluene (100ml), reevaporated, dissolved in DCM (100ml), filtered through a short pad of Kieselguhr, eluting with DCM, organic extracts evaporated, chromatographed (0-100% DCM:40-60 Petroleum ether then 0-10% methanol/DCM) to give product as an impure brown oil (427mg, 21%). MS(ES+)m/zl92(MH+). (b)6,7-Dihydro-5H-cyclopenta[c]pyridin-3-ylmethanol A solution of ethyl 6,7-dihydro-5H-cyclopenta[c]pyridine-3-carboxylate (427 mg, 2.233 mmol) in (THF) (20 ml) at -78°C under argon was treated with LiAlH4 (1M in THF)(2.233 ml, 2.233 mmol) and allowed warm to rt. After 10 min at rt, water (1ml), 2M aq NaOH (1ml) and water (1ml) were sequentially added and the mixture stirred at rt for -49- 0.5h. The mixture was then filtered through a short pad of Kieselguhr, eluting with THF (50ml), organic extracts were then evaporated, chromatographed (0-20% methanol/DCM) to give product as an orange oil (189mg, 57%). MS(ES+)m/zl50(MH+). (c) 6,7-Dihydro-5H-cyclopenta[c]pyridine-3 -carbaldehyde A solution of 6,7-dihydro-5H-cyclopenta[c]pyridin-3-ylmethanol (189 mg, 1.267 mmol) in DCM (10 ml) at rt under argon was treated with manganese dioxide (l.lOlg, 12.67 mmol) and then stirred at rt for 2h, filtered through a thin pad of Kieselguhr, eluting with DCM (40ml), the organic extracts were evaporated to give the crude product as a brown oil (1 lOmg, 59%). MS (ES+) m/z 148 (MH+). (d) Title compound A suspension of (1R)-l -[(4-amino-l -piperidinyl)methyl]-l ,2-dihydro-4H,9Himidazo[ l,2,3-ij]-l,8-naphthyridine-4,9-dione dihydrochloride (82 mg, 0.220 mmol) (for a preparation see Example 5(j)) in chloroform (5 ml) and methanol (0.2 ml) at rt under argon was treated with triethylamine (92 µl, 0.659 mmol)and stirred at rt for 0.25h. The solution was then treated with 6,7-dihydro-5H-cyclopenta[c]pyridine-3-carbaldehyde (32.3 mg, 0.220 mmol) and stirred for a further 0.5h. The solution was then treated with NaBH(OAc)3 (140 mg, 0.659 mmol) and stirred at rt for 0.5h. The reaction was then treated with saturated aqueous NaHCO (100ml) and extracted with 20% methanol/DCM (3 x 200ml). The combined organic extracts were dried (MgSO ), filtered, evaporated and chromatographed (0-20% methanol/DCM) to give the free base of the title compound as a light brown solid (39mg, 41%). MS (ES+) m/z 432 (MH+). δH (CDC13, 250MHz) 1.32-1.59 (2H, m), 1.82-2.40 (6H, m) 2.51-2.72 (3H, m), 2.82-3.18 (6H, m), 3.95 (2H, s), 4.31-4.42 (1H, m), 4.50-4.61 (1H, m), 4.92-5.08 (1H, m), 6.19-6.32 (2H, m), 7.23 (1H, s), 7.42-7.53 (2H, m), 8.38 (1H, s). The free base in DCM/MeOH 2:1 (15ml) was treated with one equivalent of 1M HC1 in diethyl ether and then evaporated to give the title monohydrochloride salt. Example 9 (1R)-l-({4-[(1,3-Dihydrofuro[3,4-c]pyridin-6-ylmethyl)amino]-lpiperidinyl} methyl)-l,2-dihydro-4H,9H-imidazo[l,2,3-H]-l,8-naphthyridine-4,9- dione hydrochloride (a) Ethyl l,3-dihydrofuro[3,4-c]pyridine-6-carboxylate -50- A solution of di-2-propyn-l-yl ether (5.01 g, 53.2 mmol) and ethyl cyanoformate (5.21 ml, 53.2 mmol) in dry degassed 1,4-dioxane (500 ml) under argon at rt was treated with cyclopentadienyl-Cobalt(I)-dicarbonyl (1.437 g, 7.98 mmol) and then heated at reflux (heating block temp 120 °C) for 18h. The reaction was evaporated, treated with toluene (100ml), re-evaporated, dissolved in DCM (100ml), filtered through a short pad of Kieselguhr, eluting with DCM, organic extracts evaporated, chromatographed (0- 100% DCM:40-60 Petroleum ether then 0-10% methanol/DCM) to give product as an impure brown solid (0.87lg) and impure product as a black oil (2.684g) which was rechromatographed (0-10-10% methanol/DCM,) to give more material as a brown solid (1.261g). Total product obtained was (2.132g, 21%). MS (ES+) m/z 194 (MH+). (b) l,3-Dihydrofuro[3,4-c]pyridin-6-ylmethanol A solution of ethyl l,3-dihydrofuro[3,4-c]pyridine-6-carboxylate (0.871 g, 4.51 mmol) in THF (20 ml) at -78°C under argon was treated with LiAlH4 (1M in THF) (4.51 ml, 4.51 mmol) and allowed warm to rt. After 10 min at rt, water (1ml), 2M aq NaOH (lml) and water (1ml) were sequentially added and the mixture stirred at rt for 0.5h. The mixture was then filtered through a short pad of Kieselguhr, eluting with THF (50ml), organic extracts were then evaporated, chromatographed (0-20% methanol/DCM) to give product as an orange oil (66mg, 10%). MS(ES+)m/zl52(MH+). (c) 1,3-Dihydrofuro[3,4-c]pyridine-6-carbaldehyde A solution of l,3-dihydrofuro[3,4-c]pyridin-6-ylmethanol (66 mg, 0.437 mmol) in DCM (5 ml) at rt under argon was treated with manganese dioxide (380 mg, 4.37 mmol) and then stirred at rt for 2h, filtered through a thin pad of Kieselguhr, eluting with DCM (40ml) and methanol (10ml), the organic extracts were evaporated to give the crude product as a brown oil (65mg, 100%) MS(ES+)m/zl50(MH+). (d) Title compound A suspension of (1R)-1 -[(4-amino-1 -piperidinyl)methyl]-1,2-dihydro-4H,9Himidazo[ l,2,3-ij]-l,8-naphthyridine-4,9-dione (121 mg, 0.324 mmol) (for a preparation see Example 5(j)) in chloroform (5 ml) and methanol (0.2 ml) at rt under argon was treated with triethylamme (0.136 ml, 0.972 mmol)and stirred at rt for 0.25h. The solution was then treated with l,3-dihydrofuro[3,4-c]pyridine-6-carbaldehyde (48.3 mg, 0.324 mmol) and stirred for a further 0.5h. The solution was then treated with NaBH(OAc)3 (206 mg, 0.972 mmol) and stirred at rt for 0.5h. The reaction was then treated with saturated aqueous NaHC03 (100ml) and extracted with 20% methanol/DCM (3 x 200ml). The combined organic extracts were dried (MgSO ), filtered, evaporated and chromatographed (0-20% methanol/DCM) to give the free base of the title compound as a light brown solid (37mg, 26%) MS (ES+) m/z 434 (MH+). - 5 1 - 8H (CDC13, 250MHz) 1.21-1.52 (2H, m), 1.78-2.00 (2H, m), 2.15-2.40 (2H, m) 2.49-3.15 (5H, m), 3.95 (2H, s), 4.31-4.48 (1H, m) 4.50-4.62 (1H, m), 4.92-5.19 (5H, m), 6.19-6.32 (2H, m), 7.27 (1H, s), 7.41-7.54 (2H, m), 8.45 (1H, s). The free base in DCM/MeOH 2:1 (15ml) was treated with one equivalent 1M HC1 in diethyl ether and then evaporated to give the title mono salt. Example 10 (1R)-1-({4-[(3,4-Dihydro-2H-pyrano[2,3-c]pyridin-6-ylmethyl)amino]- 1-piperidinyl}methyl)-1,2-dihydro-4H,9H-imidazo[1,2,3-yl-1,8-naphthyridine-4,9- dione hydrochloride A suspension of (1R)-1 -[(4-amino-1 -piperidinyl)methyl]-1,2-dihydro-4H,9Himidazo[ l,2,3-ij]-l,8-naphthyridine-4,9-dione (for a preparation see Example 5(j)) (51 mg, 0.14 mmol) in chloroform.methanol (9:1, 3 ml) at rt under argon was treated with triethylamine (0.06ml) and stirred at rt for 10 min. The solution was then treated with 1,3- dihydrofuro[3,4-c]pyridine-6-carbaldehyde (for a synthesis see WO2004058144, Example 126(e)) (21mg, 0.133mmol) and stirred for a further 2h. The solution was then treated with NaBH(OAc)3 (87mg) and stirred at rt for 2h. The reaction was then treated with saturated aqueous NaHCO (10ml) and extracted with 20% methanol/DCM (3 x 50ml). The combined organic extracts were dried (MgSO ), filtered, evaporated and chromatographed (0-20% methanol/DCM) to give the free base of the title compound as a light brown solid (20mg, 32%) MS (ES+) m/z 448 (MH+). δH (CDC13, 400MHz) 1.15-1.49 (2H, m), 1.61-1.95 (2H, m), 1.99-2.09 (2H, m) 2.20-2.38 (1H, m), 2.45-2.85 (6H, m), 2.92-3.02(lH, m), 3.05-3.15 (1H, m), 3.78 (2H, s), 4.20 (2H, t), 4.30-4.42 (1H, m), 4.52-4.61 (1H, m), 4.95-5.05 (1H, m), 6.23-6.32 (2H, m), 7.00 (1H, s), 7.47-7.50 (2H, m), 8.07 (1H, s). The free base in DCM was treated with one equivalent 1M HC1 in diethyl ether and then evaporated to give the title monohydrochloride salt. Example 11 7-[({l-[(4,9-Dioxo-1,2-dihydro-4H,9H-imidazo[l,2,3-ij]-1,8- naphthyridin-1-yl)methyl] -4-piperidinyl} amino) methyl] -2,3-dihydro-1,4- benzodioxin-5-carbonitrile hydrochloride (2:1 mixture of R:S) -52- (a) 1,1 -Dimethylethyl (1 - {(2i?)-2-hydroxy-3-[7-(methyloxy)-2-oxo-3,4-dihydro-l ,8- naphthyridin-l(2H)-yl]propyl}-4-piperidinyl)carbamate A mixture of 7-(methyloxy)-1 -[(2S)-2-oxiranylmethyl]-3,4-dihydro-1,8- naphthyridin-2(1H)-one (made according to the general method of Example 5(f) but using (2R)-2-oxiranylmethyl 3-nitrobenzenesulfonate) (3.1 g, 13.3mmol) and 1,1- dimethylethyl 4-piperidinylcarbamate (2.7g, 13.3mmol) in DMF (3ml) was heated at 110°C for lh. DMF was then evaporated. The crude product was purified by silica chromatography using a 0 - 10% methanol/dichlormethane gradient to provide the desired compound as a pale yellow solid, presumed R enantiomer (3.9g; 89%; 90% purity). MS (ES+) m/z 435 (MH+). (b) 1,1 -Dimethylethyl {1 -[(4,9-dioxo-l,2,8,9-tetrahydro-4H,7H-imidazo[l,2,3-y]-l,8- naphthyridin-2-yl)methyl]-4-piperidinyl}carbamate (2:1 mixture of R:S) A solution of 1,1-dimethylethyl (l-{(2R)-2-hydroxy-3-[7-(methyloxy)-2-oxo-3,4- dihydro-l,8-naphthyridin-l(2H)-yl]propyl}-4-piperidinyl)carbamate (3.9g, 8.97mmol) in chloroform (150ml) and triethylamine (3.1ml) under argon was treated with methanesulfonic anhydride (3.1g, 17.94mmol) at room temperature and then heated at reflux for 2.5h.The solvents were evaporated and the residue dissolved in acetonitrile (150ml) and treated with sodium iodide (6.7g, 44.85mmol) and heated at 80°C. After 45 minutes acetonitrile was evaporated and the residue was partitioned between water (250ml) and 20% methanol/dichloromethane (250ml); the layers were separated and the aqueous layer was extracted with 20% methanol/dichloromethane (4x250ml). The combined organic extracts were dried on magnesium sulphate, filtered and evaporated. The crude was purified by silica chromatography using a 0 - 10% methanol/dichloromethane gradient to provide the desired compound as a bright orange foam (1.83g; 57%, impure with triethylamine residues). The aqueous layer was evaporated and then treated with chloroform; the solid was filtered off and the chloroform was evaporated to give 2.77g of a yellow solid. The solid was dissolved in methanol and loaded onto a SCX cartridge which was pre-wet with methanol. The cartridge was washed with methanol (50ml) and then with 2M aminonia in methanol (50ml). The 2M aminonia in methanol was evaporated to afford the pure product as a white solid (220mg), presumed 2:1 mixture of R:S). MS (ES+) m/z 403(MH+). (c) 1,1 -Dimethylethyl {1 -[(4,9-dioxo-1,2-dihydro-4H,9H-imidazo[l,2,3-ij]-1,8- naphthyridin-1 -yl)methyl]-4-piperidinyl} carbamate (2:1 mixture of R:S) A mixture of 1,1 -dimethylethyl {1 -[(4,9-dioxo-l ,2,8,9-tetrahydro-4H,7Himidazo[ l,2,3-ij]-l,8-naphthyridin-2-yl)methyl]-4-piperidinyl}carbamate (2:1 mixture of R:S) (1.83g, 4.55mmol) and DDQ (1.6g, 6.83mmol) in 1,4-dioxane (100ml) was stirred at 60°C under argon overnight. More DDQ (1.6g, 6.83mmol) was added and the reaction was stirred at 60°C for another 1h. The reaction was cooled to room temperature, treated with 5% aqueous solution of potassium carbonate (600ml) and extracted with 20% - 5 3 - methanol/dichloromethane (3x500ml). The combined organic extracts were dried over magnesium sulphate, filtered and evaporated to afford the crude as a brown oil. LCMS showed that there was still - 8% of starting material left so the oil was combined with more starting material (220mg) recovered from the aqueous in the previous step and was dissolved in 1,4-dioxane (100ml), treated with 1 eq. of DDQ and heated at 60°C for lh. LCMS showed that the reaction was not complete so 0.5g of DDQ was added and the reaction was stirred at 60°C for 0.5h. A small work-up showed that the reaction was complete so the reaction was treated with 5% aqueous solution of potassium carbonate (500ml) and extracted with 20% methanol/dichloromethane (2x500ml). The combined organic extracts were dried over magnesium sulphate, filtered and evaporated to afford the product as a light brown foam (lg, 50%). MS (ES+) m/z 401 (MH+). (d)l-[(4-Amino-l-piperidinyl)methyl]-l,2-dihydro-4H,9H-irmdazo[l,2,3-ij]-1,8- naphthyridine-4,9-dione (2:1 mixture of R.S) 1,1 -Dimethylethyl {1 -[(4,9-dioxo-1,2-dihydro-4H,9H-imidazo[ 1,2,3-ij]-1,8- naphthyridin-1-yl)methyl]-4-piperidinyl}carbamate (2:1 mixture of R:S) (1g, 2.5mmol) was dissolved in chloroform (10ml) and treated with 4M HC1 in 1,4-dioxane solution (10ml) at room temperature. A solid precipitated so some methanol was added to dissolve it. After 1h LCMS showed that the reaction was complete so more methanol was added to dissolve all the solids, followed by toluene (~50ml). All the solvents were evaporated under reduced pressure to afford a yellow solid. The solid was dissolved in 100ml of methanol and stirred with Amberlyst A21 resin for 1h. The resin was then filtered off and the methanol removed to afford 0.7 g of a brown gum. The gum was dissolved in methanol and loaded onto a SCX cartridge that was pre-wet with methanol. The cartridge was washed with methanol and then with 2M aminonia in methanol. The 2M aminonia in methanol was evaporated to afford the product as a light brown gum (0.6g, 80%). MS(ES+)m/z301(MH+). Product made by this general method was analyzed via chiral HPLC (Chiralpak AS-H (5 microns) with 90:10:0.1 acetonitrile:methanol:isopropylamine as the mobile phase.). The ratio of isomers (presumed R:S) was approximately 2:1. (e) Title compound l-[(4-Amino-l-piperidinyl)methyl]-l,2-dihydro-4H,9H-imidazo[l,2,3-ij]-1,8- naphthyridine-4,9-dione (2:1 mixture of R.S) (60mg, 0.2 mmol) and 7-formyl-2,3- dihydro-l,4-benzodioxin-5-carbonitrile (for a synthesis see WO06014580 Preparation 13 or WO2007122258 Example 31(d)) (37.8mg, 0.2mmol) were dissolved in dichloromethane/methanol (2/0. lml) at room temperature under argon and stirred at room temperature for 1h. This was then treated with NaBH(OAc)3 (85mg, 0.4mmol) and left to stir for 1 hour. A saturated solution of sodium bicarbonate (15ml) was then added and the aqueous was extracted with 20%methanol/dichloromethane (3x35ml). The combined organic extracts were dried on magnesium sulphate, filtered and evaporated. The crude was purified by silica chromatography using a 0 - 20% methanol/dichloromethane -54- gradient to provide the free base of the title compound as a pale yellow gum (26mg, 27%). δH CDC13) (250MHz) 1.15-1.45 (m, 2H), 1.53 (bs, 1H),1.70-1.90 (m, 2H), 2.15-2.35 (m, 1H), 2.35-2.55 (m, 1H), 2.55-2.75 (m, 2H), 2.85-3.00 (m, 2H), 3.00-3.15 (m, 1H), 3.68 (s,2H), 4.25-4.45 (m, 5H), 4.50-4.65 (m, 1H), 4.90-5.10 (m, 1H), 6.20-6.35 (m, 2H), 7.00-7.10 (m, 2H), 7.40-7.55 (m, 2H). MS (ES+) m/z 474 (MH+). The title compound was prepared by dissolving the free base in DCM and treating it with 1 equivalent of 1M HC1 in diethyl ether. This was then evaporated to dryness and dried in the vacuum desiccator in the presence of P2O5. Example 12 l-[(4-{[(3-Oxo-3,4-dihydro-2H-1,4-benzothiazin-6-yl)methyl]amino}- l-piperidiny])methyl]-1,2-dihydro-4H,9H-imidazo[l,2,3-ij]-1,8-naphthyridine-4,9- dione hydrochloride (2:1 mixture of R:S) Ok. > k .N r-r^^yr K^. l-[(4-Amino-l-piperidinyl)methyl]-1,2-dihydro-4H,9H-imidazo[1,2,3-ij]-1,8- naphthyridine-4,9-dione (2:1 mixture of R:S, for a preparation see Example 11(d)) (60mg, 0.2 mmol) and 3-oxo-3,4-dihydro-2H-l,4-benzothiazine-6-carbaldehyde (for a synthesis see WO2002056882, Example 6(c) (38.6mg, 0.2mmol) were dissolved in dichloromethane/methanol (2/0. lml) at room temperature under argon and stirred at room temperature for lh. This was then treated with NaBH(OAc)3 (85mg, 0.4mmol) and left to stir for 1 hour. A saturated solution of sodium bicarbonate (20ml) was then added and the aqueous was extracted with 20%methanol/dichloromethane (3x35ml). The combined organic extracts were dried on magnesium sulphate, filtered and evaporated. The crude product was purified by silica chromatography using a 0-20% methanol/dichloromethane gradient to provide the free base of the title compound as a yellow solid (37mg, 39%). 5H CDC13, (250MHz) 1.20-1.45 (m, 2H),1.70-2.15 (m, 4H), 2.15-2.40 (m, 2H), 2.40-2.75 (m, 3H), 2.95 (d, 1H), 3.05-3.15 (m, 1H), 3.40 (s, 2H), 3.76 (s, 2H), 4.30-4.45(m, 1H), 4.50-4.65 (m, 1H), 4.90-5.10 (m, 1H), 6.20-6.35 (m, 2H), 6.85-7.00 (m, 2H), 7.25 (d, 1H), 7.40-7.60 (m, 2H), 8.53 (bs, 1H). MS (ES+) m/z 478 (MH+). The title compound was prepared by dissolving the free base in DCM/MeOH and treating it with 1 equivalent of 1M HC1 in diethyl ether. This was then evaporated to dryness and dried in the vacuum desiccator in the presence of P2O5 for 4 days. Example 13A(1R)-l-({4-[([l,3]Oxathiolo[5,4-clpyridin-6-ylmethyl)amino]-lpiperidinyl} methyl)-1,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione hydrochloride - 5 5 - °vrvVNV° XJ (a) 6-(Methyloxy)-3 -nitro-2-pyridinamine A solution/suspension of 2-chloro-6-(methyloxy)-3-nitropyridine (65.7 g, 348 mmol) in 2M aminonia in methanol (500 ml, 1000 mmol) and aqueous aminonia (500 ml, 348 mmol) was stirred at 65 °C for 18h. The reaction was cooled down and the solid filtered off and washed with water (2x100ml). The solid was dried in the vacuum oven at 40°C overnight to afford the product as a bright yellow solid (52.14g, 84% purity by NMR, 74%). MS(ES+)m/z 170(MH+). (b) 6-(Methyloxy)-2,3-pyridinediamine 6-(Methyloxy)-3-nitro-2-pyridinamine (26 g, 129 mmol) was suspended in ethanol (500 ml) at room temperature under argon and then treated with palladium on carbon (15 g, 14.10 mmol) (10% paste). The reaction was stirred under 1 arm of hydrogen overnight. The reaction was filtered through a Celite pad and the pad washed with ethanol (500ml). Ethanol was evaporated to afford the product as a purple oil (20.68g, slightly impure). MS (ES+) m/z 140 (MH+). (c) Ethyl N-[2-amino-6-(methyloxy)-3-pyridinyl]glycinate 6-(Methyloxy)-2,3-pyridinediamine (21.7 g, estimated 87% purity, 136 mmol) was dissolved in acetonitrile (500ml) at room temperature under argon and thenlreated with potassium carbonate (24.38 g, 176 mmol) and ethyl bromoacetate (18.13 ml, 163 mmol). The reaction was stirred at room temperature ovemight. The acetonitrile was then removed in vacuo. The reaction was repeated using more 6-(methyloxy)-2,3- pyridinediamine (20.68 g, 87% purity, 129 mmol), in acetonitrile (500ml), potassium carbonate (23.23g) and ethyl bromoacetate (17.27g) and the reaction was again stirred at room temperature overnight and the acetonitrile was then removed in vacuo. The residues were partitioned between water (1L) and ethyl acetate (1L) and the layers separated. The aqueous layer was extracted once more with ethyl acetate (1L) and the combined organic extracts were dried over MgSO4, filtered and evaporated to afford a purple oil (64g). The oil was treated with DCM (300ml) and the insoluble impurities filtered off. The DCM solution was loaded onto a 800g silica column and eluted with 0-2%MeOH/DCM to afford 40.6g of desired product as a brown solid (LCMS and NMR consistent with 75% desired product with 15% cyclized product 6-(methyloxy)-l,4-dihydropyrido[2,3- b]pyrazin-3(2H)-one and 6.4g of cyclized product 6-(methyloxy)-l,4-dihydropyrido[2,3- b]pyrazin-3(2H)-one as a purple solid. MS (ES+) m/z 226 (MH4). -56- (d) 6-(Methyloxy)-1,4-dihydropyrido[2,3-b]pyrazin-3(2H)-one Ethyl N-[2-amino-6-(methyloxy)-3-pyridinyl]glycinate (40.6 g, 135 mmol) was dissolved in tetrahydrofuran (THF) (1L) at room temperature under argon and treated with potassium tert-butoxide (15.17 g, 135 mmol). After 2h at room temperature saturated NH4CI (500ml) was added and the THF evaporated. Water (500ml) was added followed by 20%MeOH/DCM (1L); the insoluble material was filtered off, washed with diethyl ether and dried in the vacuum oven at 40°C overnight to afford the desired product as a yellow solid (15.3g): LCMS and NMR consistent with product (9% of oxidized material present by NMR). The two phases were transferred to a separating funnel and separated. The aqueous layer was extracted twice more with 20%MeOH/DCM (2x5 00ml) and the combined organic extracts were dried, MgS04 filtered and evaporated to afford a brown solid which was washed with plenty of diethyl ether to afford more of the desired product as a pale green solid (7.7g): LCMS and NMR consistent with product (20% of oxidized material present by NMR). MS(ES+)m/zl80(MH+). Alternative procedure: Ethyl N-[2-amino-6-(methyloxy)-3-pyridinyl]glycinate (16.2 g, 72mmol) was dissolved in tetrahydrofuran (500ml) and cooled to 0°C (ice bath cooling) under argon. This was then treated with potassium tert-butoxide (1M in THF, 80ml, 80mmol). After 1.5h the reaction was treated with acetic acid (80mmol) and evaporated to give a dark solid. This was triturated with water (200ml), filtered and dried in vacuo (~13g, quant.), which may be used without further purification (e) Phenylmethyl 6-(methyloxy)-3 -oxo-3,4-dihydropyrido[2,3-b]pyrazine-1 (2H)- carboxylate To 6-(methyloxy)-l,4-dihydropyrido[2,3-b]pyrazin-3(2H)-one (6.35 g, 35.4 mmol) in ethyl acetate (600 ml)/sodium bicarbonate (sat. solution) (200 ml) stirred vigorously was added at room temperature benzyl chloroformate (5.31 ml, 37.2 mmol). After 45 minutes the reaction was complete. The layers were separated and the organic layer was dried on magnesium sulphate, filtered and evaporated to afford the desired product as an off-white solid (1 lg, 99%). MS(ES+)m/z314(MH+). (f) Phenylmethyl 6-(methyloxy)-4-[(2R)-2-oxiranylmethyl]-3-oxo-3,4-dihydropyrido[2,3- bjpyrazine-1 (2H)-carboxylate Phenylmethyl 6-(methyloxy)-3-oxo-3,4-dihydropyrido[2,3-b]pyrazine-1 (2H)- carboxylate (11 g, 35.1 mmol) was dissolved in DMF (300 ml) at room temperature under argon to give a yellow solution. The solution was then cooled with an ice bath and treated with sodium hydride (1.685 g, 42.1 mmol).The solution was allowed to warm to room temperature. After 20 minutes (2S)-2-oxiranylmethyl 3-nitrobenzenesulfonate (9.56 g, 36.9 mmol) was added. After 1h all the starting material was consumed so the reaction was treated with a saturated solution of sodium bicarbonate (350ml) and the aqueous -57- layer was extracted with DCM (3x400ml). The combined organic layers were dried on magnesium sulphate, filtered and evaporated to afford a light brown oil (16.93g). The product was used as crude in the next step. MS (ES+) m/z 370 (MH+). (g)Phenylmethyl(15)-l-(hydroxymethyl)-3,8-dioxo-l,2,3,4-tetrahydro-5H,8H-2a,5,8arriazaacenaphthylene- 5 -carboxylate Phenylmethyl 6-(methyloxy)-4-[(2R)-2-oxiranylmethyl]-3-oxo-3,4- dihydropyrido[2,3-b]pyrazine-l(2H)-carboxylate (crude, 15.93 g, estimated 32.8 mmol) was dissolved in DMF (250 ml) at room temperature and heated at 130°C for 2 nights and at 120°C for one night. The reaction was complete so DMF was evaporated and the residue treated with water/brine(350/50ml) and DCM (500ml).The layers were separated and the aqueous layer was extracted once more with DCM (500ml). The combined organic extracts were dried on magnesium sulphate, filtered and evaporated to afford a brown oil which was dried under high vacuum over the weekend. The crude product was purified by silica chromatography using a 0-10%methanol/dichloromethane gradient to afford the desired product as a golden foam (3.6g, 30.9%). MS (ES+) m/z 356 (MH+). (1)(15)-1-(Hydroxymethyl)-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione Phenylmethyl(15)-1-(hydroxymethyl)-3,8-dioxo-l,2,3,4-tetrahydro-5H,8H- 2a,5,8a-triazaacenaphthylene-5-carboxylate (1.6 g, 4.50 mmol) was dissolved in ethanol (100 ml) at room temperature and then treated with palladium on carbon (10% paste) (1 g, 0.940 mmol). Everything was stirred at room temperature under 1 atm of hydrogen for 3 hours. The reaction was then filtered through a Celite pad and the impurities washed with more ethanol. The product was then eluted with DMF (400ml) and the DMF evaporated to afford a brown solid (780mg). The solid was then suspended in 30% MeOH/DCM (150ml) and stirred with manganese dioxide (1.174 g, 13.51 mmol) at room temperature for 5h and then filtered through a pad of Celite which was washed with 20% methanol/dichloromethane (100ml).The solvents were evaporated to afford the desired compound as a brown solid (750mg, 76%). MS (ES+) m/z 220 (MH+). (i)[(15)-3,8-Dioxo-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylen-l-yl]methyl methanesulfonate (15)-1 -(Hydroxymethyl)-1,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8- dione (750 mg, 3.42 mmol) was suspended in dry DCM (100 ml) at room temperature under argon and then treated with triethylamine (0.572 ml, 4.11 mmol). The mixture was then cooled using an ice-water bath. Methanesulfonyl chloride (0.293 ml, 3.76 mmol) was then added and the reaction was allowed to warm up to room temperature. After 50 minutes there was no starting material left so the mixture was washed with saturated NaHC03 (100ml). The aqueous layer was extracted with 20%MeOH/DCM (2x100ml); -58- the combined organic extracts were dried on magnesium sulphate, filtered and evaporated to afford the product as a brown foam (1.05g, 90% purity by LCMS). MS (ES+) m/z 297.9 (MH+). (j)l,l-Dimethylethyl(l-{[(1R)-3,8-dioxo-1,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylen- l-yl]methyI}-4-piperidinyl)carbamate A solution of [(l.S)-3,8-dioxo-1,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylen-l - yljmethyl methanesulfonate (1.05 g, 3.53 mmol) in dry acetonitrile (50 ml) at room temperature under argon was treated with pyridine (0.343 ml, 4.24 mmol), followed by 1,1-dimethylethyl 4-piperidinylcarbamate (0.884 g, 4.24 mmol). The mixture was heated at 70°C for 1.5h and then at 90°C for 3h. LCMS showed -25% of product. So 0.5 eq of pyridine and 0.5 eq of 1,1-dimethylethyl 4-piperidinylcarbamate were added and the reaction was heated at 90°C overnight and then stirred at room temperature for 2 days. The reaction was complete. The solvent was evaporated and the residue partitioned between sat NaHC03 and 20% methanol/dichloromethane (100ml/100ml). The layers were separated and the aqueous layer was extracted with 20% methanol/dichloromethane again (2x100ml). The combined organic extracts were dried on magnesium sulphate, filtered and evaporated to afford 1.7g of crude which was purified by silica chromatography using a 0-5% methanol/dichloromethane gradient to afford the product as a yellow solid (0.57g, 40.2%). MS(ES+)m/z402(MH+). (k) (1R)-1 -[(4-Amino-l-piperidinyl)methyl]-l ,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylene- 3,8-dionedihydrochloride A solution of 1,1-dimethylethyl (l-{[(1R)-3,8-dioxo-l,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylen- l-yl]methyl}-4-piperidinyl)carbamate (0.57 g, 1.420 mmol) in chloroform (7 ml) at room temperature was treated with 4M HCl in 1,4-dioxane (7ml). A solid precipitated out and the mixture was stirred at room temperature. After 0.5h some methanol was added to dissolve most of the solid, followed by toluene and all the solvents were removed to afford the product as a yellow solid (0.53g, 100%). MS(ES+)m/z302(MH+). (1) Title compound A suspension of (1R)-l-[(4-amino-1 -piperidinyl)methyl]-1,2-dihydro-3H,8H- 2a,5,8a-triazaacenaphthylene-3,8-dione dihydrochloride (165 mg, 0.441 mmol) in chloroform (10 ml) and methanol (0.4 ml) at room temperature under argon was treated with triethylamine (0.184 ml, 1.323 mmol) and stirred for 0.25h (the suspension turned into a solution). [l,3]Oxathiolo[5,4-c]pyridine-6-carbaldehyde (for a synthesis see WO2004058144 Example 61) (73.7 mg, 0.441 mmol) was then added and the reaction was stirred at room temperature for 0.5h. Sodium triacetoxyborohydride (280 mg, 1.323 mmol) was then added and the reaction was stirred at room temperature. After 1.5h LCMS showed that there was still some imine present in the mixture so 1 eq of sodium triacetoxyborohydride was added. After lh saturatedNaHC03 (50ml) was added -59- followed by 20% methanol/dichloromethane (80ml) and the aqueous layer was extracted and then separated from the organic layer. The aqueous layer was extracted again twice with 20% methanol/dichloromethane (2x80ml). The combined organic extracts were dried on magnesium sulphate, filtered and evaporated to afford 215mg of crude product which was purified by silica chromatography using a 0-20%methanol/dichloromethane gradient to afford the free base of the title compound as a yellow solid (185mg, 93%). δH CDC13, (250MHz) 1.20-1.45 (m, 2H),1.75-2.75 (m, 8H), 2.94 (d, 1H), 3.00-3.15 (m, 1H), 3.81 (s, 2H), 4.30-4.45 (m, 1H), 4.50-4.65 (m, 1H), 4.90-5.10 (m, 1H), 5.74(s, 2H), 6.34 (d, 1H), 7.19 (s, 1H), 7.77 (d, 1H), 7.87 (s, 1H), 7.99 (s, 1H). MS(ES+)m/z453(MH+). The title compound was prepared by dissolving the free base in methanol/dichloromethane and treating it with 1 equivalent of 1M HC1 in diethyl ether. This was then evaporated to dryness and dried in the vacuum desiccator in the presence ofP2O5. Example 13B(1R)-l-({4-[([l,3]Oxathiolo[5,4-c]pyri(lin-6-ylmethyl)aniino]-lpiperidinyl} methyl)-1-dihydro-3H,8H-2a,5,8a-triazaacenaphthyleiie-3,8-dione benzoate (1R)-l-({4-[([l,3]Oxathiolo[5,4-c]pyridin-6-ylmethyl)amino]-lpiperidinyl} methyl)-1,2-dihydro-3H,8H-2 a,5,8a-triazaacenaphthylene-3,8-dione hydrochloride (45 mg, 0.092mmol) was purified using an AD-H column with CH3CN:CH30H:.l%isopropylamine. The major peak was collected and the solvent was removed. The benzoate salt was made by dissolving the compound in MeOH and adding one equivalent of the benzoic acid. The solution stirred for 1 hour, the solvent was removed to give the product. Example 13C(1R)-1-({4-[([1,3]Oxathiolo[5,4-c]pyridin-6-y]methyl)amino]-lpiperidinyl} methyl)-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione fumarate (1R)-1 -({4-[([ 1,3]Oxathiolo[5,4-c]pyridin-6-ylmethyl)amino]-1 - piperidinyl}methyl)-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione hydrochloride (49 mg, 0.100 mmol) was dissolved in MeOH 10 mL and loaded onto a SCX cartridge 2g (pre-wet with methanol). The crude was adsorbed onto the cartridge and then the cartridge was washed with methanol (15 mL). The product was eluted using 2M NH3 in methanol (15 mL); the fraction containing the product was evaporated to afford free amine product (41.5mg, 92% recovery). LCMS and NMR consistent with product. The free amine was dissolved in a small amount of DCM/MeOH, treated with leq of fumaric acid (10.6mg) and stirred for lOminutes. Solvents were removed and the solid dried in the desiccator (P2O5) overnight to afford the product as a white solid (5 lmg, LCMS and NMR consistent with product). Example 14 (1R)-l-({4-[(2,3-Dihydro[l,4]oxathiino[2,3-c]pyridin-7- ylmethyl)amino]-1-piperidinyl}methyl)-l,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylene- 3,8-dione hydrochloride -60- vw A suspension of (1R)-l-[(4-amino-l-piperidinyl)methyl]-l,2-dihydro-3H,8H- 2a,5,8a-triazaacenaphthylene-3,8-dione dihydrochloride (160 mg, 0.428mmol) (for a preparation see Example 13(k) or 15(d)) in chloroform (10 ml) and methanol (0.400 ml) under aTgon at room temperature was treated with triethylamine (0.179 ml, 1.283mmol) and stirred for 0.25h at room temperature (everything went into solution). 2,3- Dihydro[l,4]oxathiino[2,3-c]pyridine-7-carbaldehyde (for a synthesis see WO2004058144, Example 60) (77 mg, 0.428mmol) was then added and the reaction was stirred at room temperature for 0.5h. Sodium triacetoxyborohydride (272 mg, 1.283mmol) was then added and the reaction was stirred at room temperature. After 1.5h there was still some imine present by LCMS so 1 equivalent of sodium triacetoxyborohydride was added. After lh sat NaHC03 (50ml) was added followed by 20% methanol/dichloromethane (80ml) and the aqueous was extracted and then separated from the organic layer. The aqueous layer was extracted again twice with 20% methanol/dichloromethane (2x80ml). The combined organic extracts were dried on MgS04, filtered and evaporated to afford 215mg of crude product which was purified by silica chromatography using a 0-20%methanol/dichloromethane gradient to afford the free base of the title compound as yellow foam (179mg, 90%). δH CDC13, (250MHz) 1.20-1.50 (m, 2H), 1.85 (t, 2H), 1.95-2.40 (m, 3H), 2.45-2.75 (m, 3H), 2.94 (d, 1H), 3.05-3.20 (m, 3H), 3.69 (s, 2H), 4.30-4.50 (m, 3H), 4.50-4.65 (m, 1H), 4.90-5.10 (m, 1H), 6.33, (d, 1H), 6.99 (s, 1H), 7.67 (d, 1H), 7.86 (s, 1H), 8.01 (s, 1H). MS (ES+) m/z 467 (MH+). The title compound was prepared by dissolving the free base in DCM/MeOH and treating it with 1 equivalent of 1M HC1 in diethyl ether. This was then evaporated to dryness and dried in the vacuum desiccator in the presence of P2O5. Example 15 (1R)-l-({4-[(2,3-Dihydro[l,4]dioxino[2,3-c]pyridin-7-yImethyl)amino]- l-piperidinyl}methyl)-1,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione dihydrochloride N :TifVl (a)Phenylmethyl(l,S)-1-{[(methylsulfonyl)oxy]methyl}-3,8-dioxo-l,2,3,4-tetrahydro- 5H,8H-2a,5,8a-triazaacenaphthylene-5-carboxylate Phenylmethyl (1S)-1 -(hydroxymethyl)-3,8-dioxo-l ,2,3,4-tetrahydro-5H,8H- 2a,5,8a-triazaacenaphthylene-5-carboxylate (242 mg, 0.681 mmol) (for a preparation see - 6 1 - Example 13(g)) was dissolved in DCM (10 ml) at room temperature under argon and then treated with triethylamine (0.114 ml, 0.817 mmol). The mixture was then cooled using an ice-water bath. Methanesulfonyl chloride (0.058 ml, 0.749 mmol) was then added and the reaction was allowed to warm up to room temperature. After lh the mixture was washed with sat NaHC03 (10ml). The aqueous layer was extracted with DCM (2x50ml) and the combined organic extracts dried on MgS04, filtered and evaporated to afford the product as a yellow foam (232mg, 95% purity by LCMS, 74.7%). MS (ES+) m/z 434 (MH4). (b) Phenylmethyi (1R)-1 - {[4-( {[(1,1 -dimethylethyl)oxy]carbonyl} amino)-1 - piperidinyl]methyl}-3,8-dioxo-l,2,3,4-tetrahydro-5H,8H-2a,5,8a-triazaacenaphthylene-5- carboxylate Phenylmethyi (15)-l-{[(methylsulfonyl)oxy]methyl}-3,8-dioxo-l,2,3,4- tetrahydro-5H,8H-2a,5,8a-triazaacenaphthylene-5-carboxylate (232 mg, 0.508 mmol) was dissolved in dry acetonitrile (10 ml)at room temperature under argon and treated with pyridine (0.049 ml, 0.610 mmol). 1,1-Dimethylethyl 4-piperidinylcarbamate (127 mg, 0.610 mmol) was then added and the reaction was heated at 70 °C overnight. Then 0.049ml of pyridine and 127mg of 1,1-dimethylethyl 4-piperidinylcarbamate were added to the reaction and the temperature was increased to 80°C for 8h and then the reaction was stirred at room temperature overnight. The solvent was evaporated and the residue partitioned between sat NaHCOj and DCM (50/50ml). The layers were separated and the aqueous layer was extracted with DCM again (2x50ml). The combined organic extracts were dried (MgSCu) filtered and evaporated to afford 280mg of crude product which was purified by silica chromatography using a 0-5% rnethanol/dichloromethane gradient to afford the product as a yellow gum (130mg, 47.6%). MS(ES+)m/z538(MH+). (c) 1,1 -Dimethylethyl (1 - {[(lE)-3,8-dioxo-1,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylen- 1 -yl]methyl} -4-piperidinyl)carbamate Phenylmethyi (1R)-l-{[4-({[(l,l -dimethylethyl)oxy] carbonyl} amino)-1 - piperidinyl]methyl}-3,8-dioxo-l,2,3,4-tetrahydro-5H,8H-2a,5,8a-triazaacenaphthylene-5- carboxylate (130 mg, 0.242 mmol) was dissolved in ethanol (10 ml) at room temperature and then treated with palladium on carbon (10% paste) (100 mg, 0.094 mmol). Everything was stirred at room temperature under 1 arm of hydrogen for 3 hours. The reaction was then filtered through a Celite pad and washed with more ethanol (50ml). The ethanol was evaporated to afford a yellow gum (79mg) which was dissolved in DCM (~10ml) and stirred with manganese dioxide (63.1 mg, 0.725 mmol) at room temperature overnight. Then 1.5 equivalents more of manganese dioxide were added (32mg) and reaction was stirred at room temperature for 3h. There was still starting material present by LCMS so 2 equivalents of manganese dioxide were added. The reaction was stirred at room temperature for 2h then filtered through a Celite pad. The Celite pad was washed with DCM and the solvents were evaporated to afford the product as a brown solid (76mg, 78%). -62- MS(ES+)m/z402(MH+). (d) (1R)-1 -[(4-Ammo-l -piperidinyl)methyl]-l ,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylene- 3,8-dione dihydrochloride A solution ofl,l-dimethylethyl (l-{[(1R)-3,8-dioxo-l,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylen- l-yl]methyl}-4-piperidinyl)carbamate (76 mg, 0.189 mmol) in chloroform (2 ml) at room temperature was treated with 4M HC1 in 1,4-dioxane (2ml). A solid precipitated out and the mixture was stirred room temperature. After 0.5h the reaction was complete so some methanol was added to dissolve most of the solid, followed by toluene and all the solvents were removed to afford the product as a dark yellow solid (70.9mg, 99%). MS (ES+) m/z 302 (MH+). (e) Title compound A suspension of (1R)-l-[(4-amino-l-piperidinyl)methyl]-l,2-dihydro-3H,8H- 2a,5,8a-triazaacenaphthylene-3,8-dione dihydrochloride (70mg, 0.187 mmol) in chloroform (5 ml) and methanol (0.2 ml) at room temperature under argon was treated with triethylamine (0.078 ml, 0.561 mmol) and stirred for 0.25h. Everything went into solution; 2,3-dihydro[l,4]dioxino[2,3-c]pyridine-7-carbaldehyde (for a synthesis see WO2004058144 Example 2(c) or WO03/087098 Example 19(d))) (30.9 mg, 0.187 mmol) was then added and the reaction was stirred at room temperature for 0.5h. Sodium triacetoxyborohydride (119 mg, 0.561 mmol) was then added and the reaction was stirred at room temperature. After 1.5h 1 eq more of sodium triacetoxyborohydride was added. After lh sat NaHC03 (50ml) was added followed by 20% MeOH/DCM (50ml) and the aqueous layer was extracted and then separated from the organic layer. The aqueous layer was extracted again twice with 20%MeOH/DCM (2x50ml). The combined organic extracts were dried on MgSO4, filtered and evaporated to afford 90mg of crude product which was purified by silica chromatography using a 0-20%methanol/dichloromethane gradient to afford the free base of the title compound as a pale yellow gum (60mg, 71%). δH CDC13, (250MHz) 1.25-1.50 (m, 2H), 1.86 (t, 2H), 2.10-2.75 (m, 6H), 2.93 (d, 1H), 3.00-3.15(m, 1H), 3.79 (s, 2H), 4.20-4.45 (m, 5H), 4.50-4.65 (m, 1H), 4.90-5.10 (m, 1H), 6.33 (d, 1H), 6.82 (s, 1H), 7.76 (d, 1H), 7.86 (s, 1H), 8.11 (s, 1H). MS(ES+)m/z451(MH+). The title compound was prepared by dissolving the free base in DCM/MeOH and treating it with 2 equivalents of 1M HCI in diethyl ether. This was then evaporated to dryness and dried in the vacuum desiccator in the presence of P2O5. Example 16A(2R)-2-({4-[([l,310xathio]o[5,4-c]pyridin-6-ylmethyl)amino]-lpiperidinyl} methy])-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione hydrochloride -63- (a) 2- {[6-(Methyloxy)-3-nitro-2-pyridinyl]amino}-1,3-propanediol 6-Methoxy-2-chloro-3-nitropyridine (36.94g, 195.9mmol) and 2-aminopropane- 1.3-diol (35.65g, 391.3mmol, 2 eq.) were stirrred in ethanol (500ml) at reflux under argon for 3 hours. The mixture was allowed to cool to room temperature and left overnight. The solvent was partially removed under reduced pressure (to ca. 150ml) and the resulting bright yellow slurry was poured into ice-water (1.5L) with vigorous stirring. The mixture was stirred for 1 hour then filtered with suction while cold. The solid was washed with ice-cold water (200ml) and air-dried.to give the title compound as a bright yellow solid (45.03g, 94%). LCMS showed desired product (93%) plus 7% starting material. The product was used without further purification. MS (ES+) m/z 244 (MH+) (b) N-(2,2-Dimethyl-l,3-dioxan-5-yl)-6-(methyloxy)-3-nitro-2-pyridinamine 2- {[6-(Methyloxy)-3 -nitro-2-pyridinyl] amino} -1,3 -propanediol (5 3.93 g, 228.7mmol) was stirred in 2,2-dimethoxypropane (900ml) under argon and ptoluenesulphonic acid monohydrate (l.OOg) was added. The mixture was stirred at room temperature overnight. This was diluted with dichloromethane (1L) and the resulting solution was treated with saturated aqueous sodium hydrogen carbonate (20ml) and solid sodium hydrogen carbonate (20g) with vigorous stirring (effervescence). The mixture was vigorously stirred for 20 minutes, then the remaining water was absorbed by addition of anhydrous sodium sulphate. The mixture was filtered with suction and the solids were washed with DCM (500ml). The combined filtrate plus washings were evaporated under reduced pressure to give a yellow solid which was stirred with petroleum ether (40-60°) over the weekend. The solid was isolated by filtration with suction, washed with petroleum ether (40-60°) and air-dried to give the title compound as a bright yellow solid 57.83g, 92%). MS (ES+) m/z 284 (MH+). (c)N2-(2,2-Dimethyl-l,3-dioxan-5-yl)-6-(methyloxy)-2,3-pyridinediamine N-(2,2-Dimethyl-l,3-dioxan-5-yl)-6-(methyloxy)-3-nitro-2-pyridinamine(35.00g, 123.6mmol) was divided into 2 aliquots, each of which was taken up in 1,4-dioxane (500ml) and hydrogenated over 10% Pd on carbon (paste, 1:1 w:w with water, 4.00g) under latm. hydrogen pressure, at room temperature overnight. The mixtures were -64- filtered with suction though Celite, using an argon blanket and taking care to minimise contact of the product with air. The solution was evaporated under reduced pressure to give the title compound as a deep purple oil. This was used immediately in the next step. MS (ES+) m/z 254 (MH+). (d) Ethyl JV-[2-[(2,2-dimethyl-1,3-dioxan-5-yl)amino]-6-(methyloxy)-3- pyridinyl]glycinate Crude N2-(2,2 -dimethyl-1,3-dioxan-5-yl)-6-(methyloxy)-2,3-pyridinediamine prepared in Example 16A(c) (assumed 123.6mmoI) was dissolved in anhydrous DMF (500ml) under argon and anhydrous potassium carbonate (37.56g, 2.2eq.) was added, followed by ethyl bromoacetate (12.31ml, 0.9eq.). The mixture was stirred at room temperature overnight. The solvent was removed under reduced pressure and the resulting reddish-brown slurry was partitioned between DCM (1.2L) and water (300ml). The organic phase was separated and washed with water (300ml), dried over sodium sulphate, filtered and evaporated under reduced pressure to give a dark red oil, this was taken up in a minimum of DCM and purified by column chromatography on silica (eluted with 5%-60% ethyl acetate in petroleum ether (40-60°)). Appropriate fractions were combined and evaporated under reduced pressure to give the title compound as a dark orange oil (35.42g, 84%). MS (ES+) m/z 340 (MH+) (e)4-(2,2-Dimethyl-13-dioxan-5-yl)-6-(methyloxy)-l,4-dihydropyrido[2,3-b]pyrazin- 3(2H)-one Ethyl N-[2-[(2,2-dimethyl-l,3-dioxan-5-yl)amino]-6-(methyloxy)-3- pyridinyl]glycinate (35.42g, 104.4mmol) was dissolved in dry THF (500ml) and the solution was added dropwise over 2 hours to a cooled (0°C) suspension of sodium hydride (4.173g of 60% w:w dispersion in oil, l.OOeq.) in dry THF (500ml) under argon. During the addition the colour of the suspension changed from orange to green. The mixture was sirred at 0°C for a further 15 minutes, then allowed to warm to room temperature and stirred at rt for 1 hour. The mixture was cooled to 0°C and saturated aminonium chloride (15ml) was added cautiously with vigorous stirring (effervescence observed). After effervescence had ceased, the mixture was allowed to warm to room temperature and stirred for 4 hours then diluted with ethyl acetate (500ml) and filtered with suction. The solids were washed with ethyl acetate (300ml) and the combined filtrate plus washings were evaporated under reduced pressure to give a dark brown solid. This was stirred with petroleum ether (40-60°) (500ml) plus ethyl acetate (20ml) for 2h and filtered with suction to give a lighter brown solid which was washed with petroleum ether (40-60°) (100ml) and air-dried to afford the title compound as an amorphous tan solid (25.37g, 82.8%). MS (ES+) m/z 316 (MNa+). (f)4-(2,2-Dimethyl-l,3-dioxan-5-yl)-6-(methyloxy)pyrido[2,3-6]pyrazin-3(4H)-one -65- 4-(2,2-Dimethyl-l,3-dioxan-5-yl)-6-(methyloxy)-l,4-dihydropyrido[2,3- b]pyrazin-3(2H)-one (25.37g) and activated manganese dioxide (120g, ~15eq.) were stirred in DCM (500mi) at room temperature for 2 hours then overnight. The mixture was filtered with suction and the solids were washed with DCM (2x100ml). The combined filtrate plus washings were evaporated under reduced pressure to give a brown foam; this was purified by column chromatography on silica (eluting with 0%-100% ethyl acetate in petroleum ether (40-60°)). Appropriate fractions were combined and evaporated under reduced pressure to give the title compound as a light tan solid (17.40g, 69%). MS (ES+) m/z 314 (MNa+). (g)4-[2-Hydroxy-l-(hydroxymethyl)ethyl]-6-(methyloxy)pyrido[2,3-6]pyrazin-3(4/0- one 4-(2,2-Dimethyl-l,3-dioxan-5-yl)-6-(methyloxy)pyrido[2,3-b]pyrazin-3(4H)-one (17.40 g, 59.7 mmol) was dissolved in tetrahydrofuran (THF) (220 ml) to give a dark yellow solution. 1M HC1 aq. (200ml) was added (transient blue and green colours appeared in the solution) and the now light yellow solution was stirred at room temperature for 1 hour. The mixture was concentrated to ca.300ml on a rotary evaporator using a cold water bath (some solid was precipitated during this procedure) then was stirred vigorously while solid sodium hydrogen carbonate was added in portions (caution: effervescence) until the mixture was ca. pH 8. The resulting yellow solid was collected by filtration with suction, washed with water (2x20ml) and air-dried to give the title compound as an amorphous yellow solid (13.805g, 91%). MS (ES+) m/z 252 (MH+). (h)(3,8-Dioxo-l,2,5a,8b-tetrahydro-3H,8H-2a,5,8a-triazaacenaphthylen-2-yl)methyl methanesulfonate In a 1L round-bottomed flask was placed 4-[2-hydroxy-l-(hydroxymethyl)ethyl]- 6-(methyloxy)pyrido[2,3-b]pyrazin-3(4H)-one (11.330 g, 45.1 mmol). Anhydrous chloroform (280 ml) was added, followed by triethylamine (31.4ml, 225 mmol), and methanesulfonic anhydride (31.4 g, 180 mmol) to give a dark yellow -brown solution. During addition of the methanesulphonic anhydride, an exotherm occurred which was sufficient to cause the solvent to boil. The mixture was stirred vigorously at reflux under argon for 4.5 hours. The mixture was allowed to cool to room temperature, diluted with DCM to ca. 600ml, and washed with water (200ml). The organic phase was separated, and the aqueous phase was extracted with DCM (2x200ml). The combined organic extracts were dried over anhydrous sodium sulphate,, filtered, and evaporated under reduced pressure to give crude mesylate as a dark brown oil. This was left overnight under 40-60° petroleum ether (200ml) plus DCM (50ml). The resulting solid was isolated by filtration with suction, washed with 4:1 petrokDCM (2x50ml) and air-dried to give the title compound as a brown amorphous solid (6.950g, 52%) MS (ES+) m/z 332 (MNa*), 298 (MH4). -66- (i) 1,1 -Dimethylethyl {1 -[(3,8-dioxo-1,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylen-2- yl)methyl]-4-piperidinyl}carbamate Crude (3,8-dioxo-i,2,5a,8b-tetrahydro-3H,8H-2a,5,8a-triazaacenaphthylen-2- yl)methyl methanesulfonate (6.950g, 23.38mmol) was dissolved in dry acetonitrile (200ml) and the mixture was treated with pyridine (7.55ml, 94.0 mmol) followed by 1,1- dimethylethyl 4-piperidinylcarbamate (10.30g, 51.4 mmol). The mixture was stirred at reflux under argon for 3h then at 50°C over the weekend. The mixture was then stirred at 90°C for 2 hours, then the volatiles were removed under reduced pressure and the residue was partitioned between DCM (600ml) and water (100ml). The organic phase was separated and the aqueous phase was extracted with DCM (2x200ml). The combined organic extracts were washed with water (2x100ml) dried over anhydrous sodium sulphate, filtered and evaporated under reduced pressure to give a dark tan solid; this was taken up in a minimum of 5% MeOH in DCM and chromatographed on silica, eluting with 0-10% MeOH in DCM. Appropriate fractions were combined and evaporated under reduced pressure to give the title compound as an amorphous pale tan solid (5.444g, 56.8%). MS (ES+) m/z 424 (MNa+), 402 (MH+). (j) 2-[(4-Amino-1 -piperidinyl)methyl]-1,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene- 3,8-dione (Racemic and Enantiomer 1 and 2 synthesis) Method A(Racemic synthesis): l,l-Dimethylethyl{l-[(3,8-dioxo-l,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylen- 2-yI)methyl]-4-piperid,inyl}carbamate (1.630g, 4.06 mmol) was suspended in DCM (30ml) and 4M HC1 in 1,4-dioxane (15ml) was added to give a bright yellow suspension (and gas evolution). The bright yellow mixture was allowed to stand at room temperature for 1 hour. LCMS showed no starting material remaining. The solvents were removed under reduced pressure and the residue was dried under reduced pressure overnight to give the dihydrochloride salt of the title compound as an amorphous tan solid (1.760g (> theoretical yield for the dihydrochloride owing to the presence of residual solvent). A portion of the crude dihydrochloride (0.513g) was dissolved in methanol (4ml) plus water (1ml) and applied to an SCX column (lOg) (preconditioned with 2 column volumes of methanol). The column was then eluted, under gravity, using (i) methanol (2x50ml), (ii) 0.5M aminonia in methanol (3x50ml fractions). Appropriate fractions were combined and evaporated under reduced pressure to give the crude title compound as a tan amorphous solid (410 mg), which contained methanol-insoluble material not apparent by LCMS (possibly aminonium chloride). The product was shaken with methanol (30 ml) and the suspension was filtered. The solid was washed with methanol (20 ml) and the combined filtrate and washings were evaporated under reduced pressure to give the title compound (360 mg, 87%). MS (ES+) m/z 302 (MH4). Method B -67- l,l-Dimethylethyl{l-[(3,8-dioxo-l,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylen- 2-yl)methyl]-4-piperidinyl}carbamate (9.735g, 24.25 mmol) was suspended in DCM (90ml) and 4M HC1 in 1,4-dioxane (45ml) was added to give a bright yellow suspension (and gas evolution). The bright yellow mixture was stirred at room temperature for 1 hour. The solvents were removed under reduced pressure to give the crude dihydrochloride as a bright yellow amorphous solid (10.420g) containing residual solvent) The racemic dihydrochloride (10.4g) was resolved into its two enantiomers by preparative chiral HPLC using a Chiralpak AD (20 microns) preparative column with 50:50:0.1 acetonitrile:methanol:isopropylamine as the mobile phase in three batches. The alpha value was 3.1 and baseline resolution was observed for all 3 runs. There was no overlap fraction and both enantiomers (as the free bases) were isolated in >99.8 ee each. (2i?)-2-[(4-Amino-l-piperidinyl)methyl]-l,2-dihydro-3H>8H-2a,5,8atriazaacenaphthylene- 3,8-dione (first component eluted): (3.30 g, light beige solid, chiral HPLC: 100% ee). MS (ES+) m/z 302 (MH+). Optical rotation: alpha D = -120° (C=1.00, methanol, 21.8°C). (25)-2-[(4-Amino-l -piperidinyl)methyl]-l ,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylene- 3,8-dione (second component eluted): (3.30 g, light beige solid, chiral HPLC: 99.8% ee). MS (ES+) m/z 302 (MH+). Optical rotation: alphaD = +122° (C=1.00, methanol, 21.8°C). (k) Title compound (2H)-2-[(4-Amino-1 -piperidinyl)methyl]-1,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylene- 3,8-dione (100 mg, 0.332 mmol) was stirred with [l,3]oxathiolo[5,4-c]pyridine-6-carbaldehyde (for a synthesis see WO2004058144 Example 61) (45 mg, 0.811 eq.) in chloroform:methanol (9:1, v:v, 5ml) at room temperature for 2 hours; the mixture was then treated with sodium triacetoxyborohydride (211 mg, 3.0 eq.) with vigorous stirring at room temperature for 30 mins. The mixture was quenched by addition of saturated aqueous sodium hydrogen carbonate (1ml). DCM (10ml) was added and vigorous stirring was continued for 10 mins, followed by separation of the phases (hydrophobic frit). The organic phase was evaporated under reduced pressure and the crude product was purified by column chromatography on silica (eluting with 0-12% (2M NH3 in MeOH) in DCM). Appropriate fractions were combined and evaporated under reduced pressure and dried on the vacuum line over the weekend to give the free base of the title compound as a pale yellow foam (70mg, 44.3%) MS (ES+) m/z 453 (MH+). 1HNMR (CDCl3) 8.00 (1H, s); 7.82 (1H, s); 7.77 (1H, d, J = 9.7Hz); 7.18 (1H, s); 6.39 (1H, d, J = 9.7Hz); 5.73 (2H, s); 5.03 (1H, m); 4.55 (1H, dd, J = 12.5Hz, 4.6Hz); 4.38 (1H, dd, J= 12.5Hz, 9.2Hz); 3.86 (2H, s), 3.13 (1H, dd, J - 12.9Hz, 3.5Hz); 2.93 (1H, m); 2.70 (1H, dd, J= 12.9Hz, 9.0Hz); 2.67 (1H, m); 2.50 (1H, m); 2.33 (1H, dt, J= 11.4Hz, 2.6Hz); 2.25 (1H, dt, J= 11.4Hz, 2.6Hz); 1.85 (3H, m); 1.36 (2H, m). -68- Treatment of the above free base (70mg) in DCM (1ml) with one equivalent of 1M HC1 in diethyl ether gave, after removal of solvents under reduced pressure, the title compound as a pale tan amorphous solid (75mg). MS (ES+) m/z 453 (MH4). Example 17A(2S)-2-({4-[([1,3]OxathioIo[5,4-c]pyridin-6-ylmethyl)amino]-lpiperidinyl} methyl)-1,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione hydrochloride i > N (25)-2-[(4-Amino-l H)rperidinyl.)methyl]-1,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylene- 3,8-dione (for a preparation see Example 16A(j) (100 mg, 0.332 mmol) was stirred with [13]oxathiolo[5,4-c]pyridine-6-carbaldehyde (for a synthesis see WO2004058144 Example 61) (45 mg, 0.811 eq.) in chloroform:methanol (9:1, v:v, 5ml) at room temperature for 2 hours; the mixture was then treated with sodium triacetoxyborohydride (211 mg, 3.0 eq.) with vigorous stirring at room temperature for 30 mins. The mixture was quenched by addition of saturated aqueous sodium hydrogen carbonate (1ml). DCM (10ml) was added and vigorous stirring was continued for 10 mins, followed by separation of the phases (hydrophobic frit). The organic phase was evaporated under reduced pressure and the crude product was purified by column chromatography on silica (2M NH3 in MeOH) in DCM. Appropriate fractions were combined and evaporated under reduced pressure and dried on the vacuum line over the weekend to give the free base of the title compound as a pale yellow foam (91mg, 61%). MS (ES+) m/z 453 (MH+). 1H NMR (CDCI3) (identical to that of (2R) enantiomer, Example 16A) 8.00 (1H, s); 7.82 (1H, s); 7.77 (1H, d,J= 9.7Hz); 7.18 (1H, s); 6.39 (1H, d, J= 9.7Hz); 5.73 (2H, s); 5.03 (lH,m); 4.55 (1H, dd, J= 12.5Hz, 4.6Hz); 4.38 (1H, dd, J = 12.5Hz, 9.2Hz); 3.80 (2H, s); 3.13 (1H, dd, J= 12.9Hz, 3.5Hz); 2.93 (1H, m); 2.70 (1H, dd, J= 12.9Hz, 9.0Hz); 2.67 (1H, m); 2.50 (1H, m); 2.33 (1H, d t , /= 11.4Hz, 2.6Hz); 2.25 (1H, dt, J= 11.4Hz, 2.6Hz); 1.85 (3H, m); 1.36 (2H, m). Treatment of the above free base in DCM (1ml) with one equivalent of 1M HC1 in diethyl ether gave, after removal of solvents under reduced pressure, the title compound as an amorphous yellow solid (95mg). MS (ES+) m/z 453 (MH+). -69- Example 18 2-({4-[([1,3]Oxathiolo[5,4-c}pyridia-6-yImethyl)amino]-lpiperidinyl} methyl)-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione hydrochloride Racemic 2- [(4-amino-1 -piperidinyl)methyl]-1,2-dihydro-3 H,8H-2a,5,8atriazaacenaphthylene- 3,8-dione (for a preparation see Example 16A(j)) (400 mg, 1.327 mmol) was stirred with [l,3]oxathiolo[5,4-c]pyridine-6-carbaldehyde (for a synthesis see WO2004058144 Example 61) (200 mg, 0.9 eq.) in chloroform:methanol (9:1, v:v, 15ml) at room temperature for 30 mins; the mixture was then treated with sodium triacetoxyborohydride (844 mg, 3.0 eq.) with vigorous stirring at room temperature for 30 mins. The reaction was quenched by addition of saturated aqueous sodium hydrogen carbonate (5 ml) and the mixture was stirred at room temperature for 5 mins. The organic phase was separated, dried over anhydrous Na2SO4 and evaporated under reduced pressure. The crude product was purified by column chromatography on silica (eluting with 0-12% (2M aminonia in methanol) in DCM, appropriate fractions were combined and evaporated under reduced pressure to give the free base of the (racemic) title compound as a pale yellow foam (290 mg, 46.8%). MS(ES+)m/z453(MH+). 1H NMR (CDCl3) (identical to those of the homochiral samples (Example 16A and 17A) except for the position of the NH) 8.00 (1H, s); 7.82 (1H, s); 7.77 (1H, d, J= 9.7Hz); 7.18 (1H, s); 6.39 (1H, d, J= 9.7Hz); 5.73 (2H, s); 5.03 (1H, m); 4.55 (1H, dd, J= 12.5Hz, 4.6Hz); 4.38 (1H, dd, J = 12.5Hz, 9.2Hz); 3.80 (2H, s); 3.13 (1H, dd, J= 12.9Hz, 3.5Hz); 2.93 (1H, m); 2.70 (1H, dd, J = 12.9Hz, 9.0Hz); 2.67 (1H, m); 2.50 (1H, m); 2.33 (1H, dt, J= 11.4Hz, 2.6Hz); 2.25 (1H, dt, J= 11.4Hz, 2.6Hz); 1.85 (2H, m); (NH under HOD peak at 1.70); 1.36 (2H,m). The free base (290mg) was dissolved in DCM (5ml) and treated with one equivalent of 1M HC1 in diethyl ether. Evaporation of the solvents under reduced pessure gave the title compound as a pale yellow amorphous solid (281mg). MS (ES+) m/z 453 (MH+) Example 16B (2R)-2-({4-[([l,3]Oxathiolo[5,4-c]pyridin-6-ylmethyl)amino]-lpiperidinyl} methyl)-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione benzoate and -70- Example 17B(25)-2-({4-[([1310xathiolo[5,4-clpyrklin-6-ylmethyl)amino]-lpiperidinyl} methyl)-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione benzoate Racemic 2-({4-[([ 1,3]oxathiolo[5.4-c]pyridin-6-ylmethyl)amino]-1 - piperidinyl}methyl)-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione hydrochloride, 200mg, was resolved into its two enantiomers by preparative chiral HPLC (using a 21 x 250mm Chiralpak IA, (5 microns) preparative column) with 1:1 acetonitrile(containing 0.1% isopropylamine) and acetonitrile (containing 0.1% TFA) as the mobile phase. (2R)-2-({4-[([l,3]Oxathiolo[5,4-c]pyridin-6-ylmethyl)amino]-l-piperidinyl}methyl)-1,2- dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione (first component eluted): (81mg) Optical rotation: {alpha}D at 23.9°C= -85.58° (C=798 in MeOH) MS (ES+) m/z 302 (MH+). The free base was dissolved in methanol and treated with benzoic acid (1 equivalent). Evaporation of the solvents under reduced pressure gave the product (Example 16B) as the benzoate salt. (2S)-2-( {4-[([ 1,3]Oxathiolo[5,4-c]pyridin-6-ylmethyl)amino]-1 -piperidinyl} methyl)-1,2- dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione (second component eluted): (76mg) Optical rotation: {alpha}D at 23.9°C= +84.9° (0.798 in MeOH) MS (ES+) m/z 302 (MH4). The free base was dissolved in methanol and treated with benzoic acid (1 equivalent). Evaporation of the solvents under reduced pressure gave the product (Example 17B) as the benzoate salt. Example 19A(2R)-2-({4-[(2,3-Dihydro[l,4]oxathiino[2,3-c]pyridin-7- ylmethyl)amino]-1-piperidinyl}methyl)-l,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylene- 3,8-dione hydrochloride (2R)-2-[(4-Amino-l-piperidinyl)methyl]-l,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylene- 3,8-dione (for a preparation see Example 16A(j)) (600mg,1.991 mmol), 2,3-dihydro[l,4]oxathiino[2,3-c]pyridine-7-carbaldehyde (for a synthesis see WO2004058144, Example 60) (325mg, 0.900 eq.) and 20µL acetic acid were stirred in - 7 1 - chloroform:methanol (9:1, v:v, 30ml) at room temperature for 2 hours; the mixture was then treated with sodium triacetoxyborohydride (1.266g, 3.0 eq.) with vigorous stirring at room temperature for 30 mins. The mixture was quenched by addition of saturated aqueous sodium hydrogen carbonate (6ml). DCM (60ml) was added and vigorous stirring was continued for 10 mins, followed by separation of the phases (hydrophobic frit). The organic phase was evaporated under reduced pressure and the crude product was purified by column chromatography on silica (eluting with 0-12% (2M NH3 in MeOH) in DCM) Appropriate fractions were combined and evaporated under reduced pressure and dried under vacuum overnight to give the free base of the title compound as a pale yellow amorphous solid (658mg). MS (ES+) m/z 467 (MH+). 1 H N M R (CDCL3, 400MHz) 1.25-1.40 (2H, m), 1.80-1.90 (2H, m), 2.20-2.30 (1H, m),2.30-2.40 (1H, m), 2.45-2.55 (1H, m), 2.65-2.75 (2H, m), 2.90-2.95 (1H, m), 3.10- 3.20 (3H, m), 3.75 (2H, s), 4.35-4.45 (3H, m), 4.50-4.60 (1H, dd), 5.00-5.10 (1H, m), 6.40 (1H, d), 7.00 (1H, s), 7.75 (1H, d), 7.85 (1H, s), 8.05 (1H, s). The free base (650mg, 1.393mmol) was suspended in dry DCM (10ml) and a 1M solution of hydrogen chloride in diethyl ether (1393uL, l.OOOeq.) was added. The system was kept sealed and shaken for 1 minute then the solvents were removed under reduced pressure and the residue was dried under vacuum to give the title compound as an amorphous yellow solid (682mg). Example 20 2-({4-[(2,3-Dihydro[l,4]oxathiino[23-c]pyridin-7-ylrnethyl)amino]-lpiperidinyl} methyl)-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione hydrochloride Example 19B(2R)-2-({4-[(2,3-Dihydro[l,4]oxathuno[2,3-c]pyridin-7- ylmethyl)amino]-l-piperidinyl}methyl)-l,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylene- 3,8-dionetrifluoroacetate and Example 21 (2S)-2-({4-[(2,3-Dihydro[l,4]oxathiino[2,3-c]pyridin-7- ylmethy])amino]-l-piperidinyl}methy])-l,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylene- 3,8-dione HN ,NL ^N TTT ^ - ^ " -72- Racemic 2-[(4-amino-1 -piperidinyl)methylj-1,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylene- 3,8-dione (for a preparation see Example 16A(j)) (360 mg, 1.195 mmol) was stirred with 2,3-dihydro[l,4]oxathiino[2,3-c]pyridine-7-carbaldehyde (for a synthesis see WO2004058144, Example 60) (195 mg, 0.9 eq.) in chloroform:methanol (9:1, v:v, 15ml) at room temperature for 30 mins; the mixture was then treated with sodium triacetoxyborohydride (760 mg, 3.0 eq.) with vigorous stirring at room temperature for 30 mins. The reaction was quenched by addition of saturated aqueous sodium hydrogen carbonate (5 ml) and the mixture was stirred at room temperature for 5 mins. The organic phase was separated, dried over anhydrous Na SO and evaporated under reduced pressure. The crude product was purified by column chromatography on silica (eluting with 0-12% (2M aminonia in methanol) in DCM, appropriate fractions were combined and evaporated under reduced pressure to give the free base of the title compound as a pale yellow foam (235 mg, 42%). NMR and LC-MS identical to product of Example 19A. The free base (225 mg) was dissolved in DCM (5ml) and treated with one equivalent of 1M HC1 in diethyl ether. Evaporation of the solvents under reduced pressure gave the title compound (Example 20) as a pale yellow amorphous solid (224mg). MS(ES+)m/z467(MH+). The title racemic hydrochloride (Example 20), 80 mg, was resolved into its two enantiomers by preparative chiral HPLC (using a 21 x 250mm Chiralpak LA, (5 microns) preparative column) with 2:2:1 methanol:acetomtrile:r-butanol (containing 0.1% isopropylamine) as the mobile phase. (2i?)-2-({4-[(2,3-Dihydro[l,4]oxathiino[2,3-c]pyridin-7-ylmethyl)amino]-lpiperidinyl} methyl)-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione (first component eluted): (31mg) MS (ES+) m/z 467 (MH+). The 2R material was of 98.7% purity; further purification was effected by reversephase HPLC on a Kromasil 5micron C-18 column (21mm x 250mm) eluted with 9:1 water (+0.1% TFA) and acetonitrile (+0.1% TFA) (3 runs) to give the di-trifluoroacetate salt (Example 19B). (25)-2-({4-[(2,3-Dihydro[l,4]oxathiino[2,3-c]pyridin-7-ylmethyl)amino]-lpiperidinyl} methyl)-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione (second component eluted): (32mg) (Example 21). The stereochemistry of this compound was determined by small molecule x-ray crystallography.MS (ES+) m/z 467 (MH+). Example 22 2-({4-[(2,3-Dihydro[l,4]dioxino[2,3-c)pyridin-7-yImethyl)amino]-lpiperidinyl} methyl)-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione hydrochloride -73- N Racemic 2-[(4-amino-l -piperidinyl)methyl]-l ,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylene- 3,8-dione (for a preparation see Example 16A(j)) (50mg, 0.166 mmol) was stirred in 9:1 v:v chloroform:methanol (2ml) with 2,3- dihydro[l,4]dioxino[2,3-c]pyridine-7-carbaldehyde (for a synthesis see WO2004058144 Example 2(c) or WO03/087098 Example 19(d)) (28mg, 1.0 equivalent) at room temperature for 30 minutes, then the mixture was treated with sodium triacetoxyborohydride (105mg, 3.0 equivalents) with vigorous stirring. After a further 25 minutes stirring, the reaction was quenched by addition of saturated aqueous sodium hydrogen carbonate (2ml), diluted with dichloromethane and stirred vigorously at room temperature for 20 minutes. The organic phase was separated (hydrophobic frit) and evaporated under reduced pressure to give an orange gum; this was purified by column chromatography on silica (eluting with 0-12% (2M NH3 in MeOH) in DCM). Appropriate fractions were combined and evaporated under reduced pressure to give the free base of the title compound as a cream amorphous solid (30mg, 40%). MS(ES+)m/z451(MH+). 1H NMR (CD3OD) δ 7.98 (1H, s); 7.88 (1H, d, J= 9.7Hz); 7.77 (1H, s); 6.94 (1H, s); 6.37 (1H, d, J= 9.7Hz); 5.12 (1H, m); 4.43 (2H, m); 4.35 (2H, m); 4.29 (2H, m); 3.73 (2H, s); 3.07 (1H, m); 3.03 (1H, m); 2.83 (1H, dd, J= 13.2Hz, 8.3Hz); 2.70 (1H, m); 2.44 (1H, m); 2.26 (1H, dt, J= 11.6Hz, 2.4Hz); 2.18 (1H, dt,7= 11.6Hz, 2.4Hz); 1.85 (2H, m);1.33(2H,m). The free base was dissolved in DCM (1ml) and treated with a 1M solution of hydrogen chloride in diethyl ether (67 juL, 1.0 equivalent); the vessel was sealed and kept at room temperature for 5 minutes then the solvents were removed under reduced pressure to give the title compound (20mg; some product lost due to splashing on evaporation of solvents). MS(ES+)m/z451(MH+). Example 23 (1R)-l-({4-[(2,3-Dihydro[l,4]oxathuno[23-c]pyridin-7- ylmethyl)amino]-4-methyl-l-piperidinyl}methyl)-l,2-dihydro-4H,9H-imidazo[l,2r3- ij]-l,8-naphthyridine-4,9-dione hydrochloride -74- XXJ (a) 1,1 -Dimethylethyl 4-methyl-4-({[(phenylmethyl)oxy]carbonyl} amino)-1 - piperidinecarboxylate A solution of l-{[(l,l-dimethylethyl)oxy]carbonyl}-4-methyl-4- piperidinecarboxylic acid (12.092 g, 49.7 mmol) in toluene (300 ml) was treated with triethylamine(13.85 ml, 99 mmol) and then diphenylphosphoryl azide (21.42 ml, 99 mmol), heated to 90 °C for 2h (bubbling observed) before treatment with benzyl alcohol (10.34 ml, 99 mmol). The reaction was then heated at 90 °C for a further 18h, then cooled, treated with saturated aqueous sodium bicarbonate (500ml), the organic extracts were separated and the aqueous extracted with diethyl ether (200ml), the combined organic extracts were dried (MgS04), filtered, evaporated, columned (0-50% ethyl acetate:40-60 petroleum ether, Rf = 0.4 in 4:1 ethyl acetate:40-60 petroleum ether) to give product as a clear oil (15.473g, 89%). (b) Phenylmethyl (4-methyl-4-piperidinyl)carbamate A solution of 1,1-dimethylethyl 4-methyl-4- ({[(phenylmethyl)oxy]carbonyl}amino)-l-piperidinecarboxylate(15.473 g, 44.4 mmol) in DCM (50 ml) under argon at rt, was treated with trifluoroacetic acid (50 ml, 649 mmol) and stirred at rt for 0.5h. The reaction mixture was then evaporated, dissolved in water (200ml), washed with diethyl ether (3x 200ml). The aqueous phase was then basified with solid potassium carbonate, extracted with 20% methanol/DCM (3 x 200ml), these organic extracts were then dried (MgSO4), filtered and evaporated to give the product as a yellow oil (6.327g, 57%). MS (ES+) m/z 249 (MH+). (c) Phenylmethyl (1 - {[(2R)-4,9-dioxo-1,2,8,9-tetrahydro-4H,7H-imidazo[ 1,2,3-zj']-1,8- naphthyridin-2-yl]methyl}-4-methyl-4-piperidinyl)carbamate A solution of (15)-l-(hydroxymethyl)-l,2,5,6-tetrahydro-4H,9H-imidazo[l,2,3- ij]-l,8-naphthyridine-4,9-dione (for a preparation see Example 5A(g)) (1.494g, 6.78 mmol) in DCM (50 ml) at 0 °C under argon was treated with triethylamine (1.7 ml, 12.20 mmol) and then methanesulfonyl chloride (0.800 ml, 10.27 mmol) and then allowed to warm to rt and stirred at rt for lh. The reaction mixture was then treated with saturated aqeous bicarbonate (200ml) and the mixture was extracted with DCM (3 x 200ml). The combined organic solvents were then dried (MgSC^), filtered, evaporated to give the crude mesylate (2.082g, 6.987mmol, 103% crude yield). The mesylate was dissolved in dry acetonitrile (30 ml) and then treated with pyridine (1.097 ml, 13-57 mmol) and a solution of phenylmethyl (4-methyl-4-piperidinyl)carbamate (3.164g, 12.74 mmol) in dry acetonitrile (20ml) and heated at reflux (heating block 95°C) for 6h. The reaction mixture -75- was then evaporated, treated with saturated aqueous NaHCC^ (200ml) and the mixture was extracted with DCM (3 x 200ml). The combined organic solvents were then dried (MgSO4), filtered, evaporated to give the crude product as an orange solid which was then chromatographed (0-10% methanol/DCM, Rf = 0.5 in 10% methanol/DCM) to give product as a yellow solid (1.848g, 61%). MS(ES+)m/z451(MH+). (d) (\R)-1 -[(4-Amino-4-methyl-1 -piperidinyl)methyl]-1,2,5,6-tetrahydro-4H,9Himidazo[ l ,2,3-ij]-1,8-naphthyridine-4,9-dione A solution of phenylmethyl (l-{[(2R)-4,9-dioxo-l,2,8,9-tetrahydro-4H,7Himidazo[ l,2,3-ij]-l,8-naphthyridin-2-yl]methyl}-4-methyl-4-piperidinyl)carbamate (1.848 g, 4.10 mmol) in ethanol at rt under argon was treated with palladium on carbon (10%o paste) (0.462 g, 4.34 mmol) (20%w/w) and stirred under 1 atmosphere of hydrogen for 2h, reaction mixture was filtered through a thin pad of Kielselguhr eluting with ethanol (100ml). The filtrate was treated with palladium on carbon (10% paste) (0.462 g, 4.34 mmol) and and stirred under 1 arm of hydrogen for 18h.The reaction mixture was filtered through a thin pad of Kielselguhr eluting with ethanol (500ml) and the filtrate was then evaporated to give the product as a yellow solid (1.294g, 100%). MS(ES+)m/z317(MH+). (e)(1R)-l-[(4-Isocyanato-4-methyl-l-piperidinyl)methyl]-l,2,5,6-tetrahydro-4iy,9Himidazo[ l ,2,3-ij]-1 ,8-naphthyridine-4,9-dione A solution of (1R)-l-[(4-amino-4-methyl-l-piperidinyl)methyri-1,2,5,6- tetrahydro-4H,9H-imidazo[l,2,3-ij]-l,8-naphthyridine-4,9-dione (1.294 g, 4.09 mmol) in DCM (30 ml) under argon at rt was treated with triethylamine (0.684 ml, 4.91 mmol), then di-tert-butyl dicarbonate (1.045 ml, 4.50 mmol) and finally 4- dimethylaminopyridine (0.050 g, 0.409 mmol) and stirred at rt for lh. The reaction mixture was treated with aq sodium bicarbonate (100ml) and extracted with DCM (3x 200ml). The combined organic fractions were dried (MgSO4), filtered and evaporated to give the crude product as a yellow solid which was then chromatographed (0-10% methanol/DCM, Rf = 0.5 in 10% methanol/DCM) to give the product as a white solid (561mg, 40%). MS (ES+) m/z 343 (MH+). (f) (IR)-1 -[(4-Amino-4-methyl-1 -piperidinyl)methyl]-l ,2,5,6-tetrahydro-4H,9Himidazo[ l ,2,3-ij]-1,8-naphthyridine-4,9-dione A solution of (lR)-l-[(4-isocyanato-4-methyl-l-piperidinyl)methyl]-1,2,5,6- tetrahydro-4H,9H-imidazo[l,2,3-ij]-l,8-naphthyridine-4,9-dione(561 mg, 1.638 mmol) in THF (10 ml) and water (10.00 ml) at rt was treated with sodium hydroxide (5 ml, 10.00 mmol), and stirred at rt for lh. Reaction was then treated with concentrated HC1 (5ml, 12M) and stirred at rt for 18h, then evaporated. The resultant sorid was treated with methanol (20ml) and then the solvent was decanted from the solid and evaporated to give the product as an impure green solid (687mg, 108%). -76- MS(ES+)m/z 317(MH+). (g) N-(l- {[(2R)-4,9-Dioxo-1,2,8,9-tetrahydro-4H,7H-imidazotl ,2,3-ij]-i ,8-naphthyridin- 2-yl]methyl}-4-methyl-4-piperidinyl)-2,2,2-trifiuoroacetamide A solution of (IB)-1 ~[(4-amino-4-methyl-1 -piperidinyl)methylj-1,2,5,6- tetrahydro-4H,9H-imidazo[l,2,3-ij]-l,8-naphthyridine-4,9-dione (687 mg, 1.765 mmol) in DCM (20 ml) and triethylamine (1.476 ml, 10.59 mmol) at 0 °C under argon was treated with trifluoroacetic anhydride (0.299 ml, 2.118 mmol) and stirred at rt for Ih. The reaction was treated with saturated sodium bicarbonate (50ml) and extracted with DCM (3 x 100ml). The combined organic solvents were then dried (MgSO), filtered, evaporated to give the crude product as an orange solid which was then chromatographed (0-10% methanol/DCM, Rf = 0.4 in 10% methanol/DCM) to give product as an impure yellow solid (375mg, 52%). MS(ES+)m/z413(MH+). (h) N-(1-{ [(1R)-4,9-Dioxo-1,2-dihydro-4H,9H-imidazo[ 1,2,3-ij]-1,8-naphthyridin-1 - yl]methyl}-4-methyl-4-piperidinyl)-2,2,2-trifluoroacetamide A solution of N-(l-{[(2R)-4,9-dioxo-1,2,8,9-tetrahydro-4H,7H-imidazo[ 1,2,3-ij]- l,8-naphthyridin-2-yl]methyl}-4-methyl-4-piperidmyl)-2,2,2-trifluoroacetamide(375mg, 0.909 mmol) in 1,4-dioxane (20 ml) at rt was treated with DDQ (248 mg, 1.091 mmol) and then heated at 80°C for 1h. The reaction was then cooled to rt. The reaction mixture was treated with saturated aqeous K2CO3 (5%, 100ml), then with DCM (100ml) and the mixture filtered through Kieselguhr. The organic fraction was separated and the aqueous layer extracted with DCM (2 x 100ml). The combined organic solvents were then dried (MgSO4), filtered and evaporated to give the crude product as a yellow oil. Chromatography on silica (0-10% methanoi:DCM, Rf = 0.4 in 10% MeOH/DCM) gave the product as a clear oil (171mg, 46%). MS(ES+)m/z411(MH+). (i)(1R)-l-[(4-Amino-4-methyl-1-piperidinyl)methyl]-l,2-dihydro-4H,9H-imidazo[l,2,3- ij]-l ,8-naphthyridine-4,9-dione N-(l-{[(1R)-4,9-Dioxo-l,2-dihydro-4H,9H-imidazo[l,2,3-ij]-l,8-naphthyridin-lyl] methyl}-4-methyl-4-piperidinyl)-2,2,2-trifluoroacetamide (171 mg, 0.417 mmol) was treated with a 7% solution of potassium carbonate (450mg in 2ml water/5ml methanol) and stirred at rt for 2h, and then at 70 °C for 18h then evaporated and dissolved in 5% MeOH/DCM (100ml), filtered and purified by SCX (5g, during with MeOH and then 0.5M NEb/MeOH and then 2M NH3/MeOH). Fractions containing product were then evaporated to give product as a pink solid (60mg, 46%) MS(ES+)m/z315(MH+). (j) Title compound A suspension of (1R)-1 -[(4-amino-4-methyl-1 -piperidinyl)methyl]-1,2-dihydro- 4H,9H-imidazo[l,2,3-ij]-l,8-naphthyridine-4,9-dione (34 mg, 0.108 mmol) in chloroform -77- (2 ml) and methanol (0.1 ml) at rt under argon was treated with 2,3- dihydro[l,4]oxathiino[2,3-c]pyridine-7-carbaldehyde (19.60 mg, 0.108 mmol) (for a synthesis see WO2004058144, Example 60) and stirred for 2h. The solution was then treated with sodium triacetoxyborohydride (68.8 mg, 0.324 mmol) and stirred at rt for 0.5h. The reaction was then treated with saturated aqueous NaHCO (20ml) and extracted with 20% methanol/DCM (3 x 100ml). The combined organic extracts were dried (MgSO), filtered, evaporated and chromatographed (0-20% methanol/DCM, Rf= 0.4 in 15% methanol/DCM) to give the free base of the title compound as a light brown solid (32mg, 0.067mmol, 62%). MS(ES+)m/z517(MH+). δH (CDC13, 250MHz) 1.14 (3H, s), 1.42-1.70 (4H, m), 2.30-2.45 (1H, m), 2.50-2.82 (4H, m), 3.05-3.22 (3H, m), 3.68 (2H, s), 4.28-4.48 (3H, m) 4.51-4.63 (1H, m), 4.96-5.11 (1H, m), 6.20-6.35 (2H, m), 7.04 (1H, s), 7.46-7.51 (2H, m), 8.00 (1H, s). The free base in DCM/MeOH 2:1 (10ml) was treated with 1M HC1 in diethyl ether and then evaporated to give the title mono-hydrochloride salt as a white solid (34mg) Example 24 (1R)-l-({4-Methyl-4-[([l,3]oxathiolo[5,4-c]pyridin-6-ylmethyl)amino]- l-piperidinyl}methyl)-l,2-dihydro-4H,9H-imidazo[l,23-ij]-1,8-naphthyridine-4,9- dione hydrochloride A suspension of (1R)-1 -[(4-amino-4-methyl-1 -piperidinyl)methyl]-1,2-dihydro- 4H,9F-imidazo[l,2,3-ij]-l,8-naphthyridine-4,9-dione (26 mg, 0.083 mmol) (for a preparation see Example 23(i)) in chloroform (2 ml) and methanol (0.1 ml) at rt under argon was treated with [l,3]oxathiolo[5,4-c]pyridine-6-carbaldehyde (13.83 mg, 0.083 mmol) (for a synthesis see WO2004058144 Example 61) and stirred at rt for 2h. The solution was then treated with sodium triacetoxyborohydride (52.6 mg, 0.248 mmol) and stirred at rt for 0.5h. The reaction was then treated with saturated aqueous NaHCO (10ml) and extracted with 20% methanol/DCM (3 x 50ml). The combined organic fractions were dried (MgSO ), filtered, evaporated and chromatographed (0-20% methanol/DCM, Rf = 0.3 in 15% methanol/DCM) to give the free base of the title compound as a yellow solid (29mg, 75%,). MS (ES+) m/z 466(MH+). δH (CDCl3, 250MHz) 1.14 (3H, s), 1.40-1.71 (4H, m), 2.30-2.46 (1H, m), 2.51-2.82 (4H, m), 3.08-3.22 (1H, m), 3.73 (2H, s), 4.28-4.42 (1H, m) 4.51-4.65 (1H, m), 4.92-5.09 (1H, m), 5.72 (2H, s), 6.20-6.34 (2H, m), 7.25 (1H, s), 7.50-7.51 (2H, m), 7.98 (IK, s). The free base in DCM/MeOH 2:1 (5ml) was treated with 1M HC1 in diethyl ether and then evaporated to give the title mono-hydrochloride salt. -78- Example 25 (2R)-2-({4-[(2,l,3-BenzothiadiazoI-5-ylmethyl)amino]-lpiperidinyl} methy])-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione hydrochloride (2R)-2-[(4-Amino-l-piperidinyl)methyl]-l,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylene- 3,8-dione (50mg, 0.166mmol) (for a preparation see Example 16(j)) and 2,l,3-henzothiadiazole-5-carbaldehyde (25mg, 0.918eq.) were stirred in 9:1 v:v chloroform:methanol (1ml) for 2.5 hours. Sodium triacetoxyborohydride (105mg, 3.000eq.) was then added in one portion and the mixture was stirred vigorously at room temperature for 30 min. Saturated aqueous sodium hydrogen carbonate (0.5ml) was then added, followed by dichloromethane (10ml) and the mixture was stirred vigorously at room temperature for 10 min and the phases were separated (hydrophobic frit). The organic phase was evaporated under reduced pressure and the crude product was purified by column chromatography on silica (eluted with 0-12% (2M NH3 in MeOH) in DCM). Appropriate fractions were combined and evaporated under reduced pressure to give the free base of the title compound as a yellow solid (41mg). MS(ES+)m/z450(MH+). lH NMR (CDCl3): δ 7.95 (1H, d, J = 9.0Hz); 7.90 (1H, s); 7.83 (1H, s); 7.77 (1H, d, J = 9.7Hz); 7.61 (1H, dd, J = 9.2Hz, 1.8Hz); 6.39 (1H, d, J= 9.7Hz); 5.03 (1H, m); 4.56 (1H, dd, J= 12.5Hz, 4.6Hz); 4.39 (1H, dd, J= 12.5Hz, 9.2Hz); 3.98 (2H, s); 3.14 (1H, dd, J = 13.2Hz, 3.5Hz); 2.94 (1H, broad m); 2.69 (2H, m); 2.56 (1H, m); 2.34 (1H, dt, J= 11.4Hz, 2.6Hz); 2.25 (1H, dt, J= 11.4Hz, 2.6Hz); 1.89 (2H, m); (NH under HOD peak at 1.48); 1.37 (2H,m). The free base (35mg, 0.078mmol) was dissolved in DCM (1ml) and hydrogen chloride (1.0M) in diethyl ether (78µL, 1.0 eq. ) was added. The system was sealed and shaken at room temperature for 1 minute, then the solvents were removed under reduced pressure to give the title compound as a yellow solid (38mg). MS (ES+) m/z 450(MH+). Example 26 (2R)-2-[(4-{[(7-Fluoro-2,3-dihydro-l,4-benzodioxin-6- yl)methyl]amino}-l-piperidinyl)methyl]-l,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylene- 3,8-dione hydrochloride -79- (2R)-2-[(4-Amino-1 -piperidinyl)methyl]-1,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylene- 3,8-dione (50mg, 0.166mmol) (for a preparation see Example 16(j)) and 7-fluoro-2,3-dihydro-benzo[l,4]dioxin-6-carboxaldehyde (28mg, 0.926eq.) (for a synthesis see WO2002056882, Example 23(a)) were stirred in 9:1 v:v chloroform:methanol (1ml) for 2.5 hours. Sodium triacetoxyborohydride (105mg, 3.000eq.) was then added in one portion and the mixture was stirred vigorously at room temperature for 30 min. Saturated aqueous sodium hydrogen carbonate (0.5ml) was then added, followed by dichloromethane (10ml) and the mixture was stirred vigorously at room temperature for 10 min and the phases were separated (hydrophobic frit). The organic phase was evaporated under reduced pressure and the crude product was purified by column chromatography on silica (eluted with 0-12% (2M NH3 in MeOH) in DCM. Appropriate fractions were combined and evaporated under reduced pressure to give the free base of the title compound as a white solid (48mg). MS (ES+) m/z 468(MH+). 1H NMR (CDCI3): δ 7.82 (1H, s); 7.76 (1H, d,J= 9.7Hz); 6.79 (1H, d, J = 7.2Hz); 6.57 (1H, d, J= 10.5Hz); 6.38 (1H, d,7= 9.7Hz); 5.03 (1H, m); 4.54 (1H, dd, J= 12.5Hz, 4.4Hz); 4.38 (1H, dd, J= 12.5Hz, 9.4Hz); 4.23 (4H, m); 3.71 (2H, s); 3.12 (1H, dd, J= 12.9Hz, 3.3Hz); 2.92 (1H, m); 2.68 (2H, m); 2.68 (2H, m); 2.47 (1H, m); 2.33 (1H, dt, J = 11.4Hz, 2.6Hz); 2.24 (1H, dt, J = 11.4Hz, 2.6Hz); 1.83 (2H, m); (NH under HOD peak at 1.50); 1.33 (2H,m). The free base (48mg, 0.103mmol) was dissolved in DCM (1ml) and hydrogen chloride (1.0M) in diethyl ether (103µL, 1.0 eq. ) was added. The system was sealed and shaken at room temperature for 1 minute, then the solvents were removed under reduced pressure to give the title compound as a yellow solid (55mg) MS (ES+) m/z 468(MH+). Example 27 (2R)-2-({4-[(3,4-Dihydro-2H-[l,4]oxathiepino[2,3-c]pyridin-8- ylmethyl)aminol-1-piperidinyl}methyl)-1,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylene- 3,8-dione hydrochloride -80- xO N (2R)-2-[(4-Amino-l-piperidinyl)methyl]-l,2-dihydro-3H)8H-2a,5,8atriazaacenaphthylene- 3,8-dione (50mg, 0.166mmol) (for a preparation see Example 16(j)) and 3,4-dihydro-2H-[l,4]oxathiepino[2,3-c]pyridine-8-carbaldehyde (29mg, 0.895eq.) (maybe prepared analogously to the synthesis of 2,3-dihydro[l,4]oxathiino[2,3- c]pyridine-7-carbaldehyde (WO2004058144, Example 60) but replacing dibromoethane with dibromopropane) were stirred in 9:1 v.v chloroform:methanol (1ml) for 2.5 hours. Sodium triacetoxyborohydride (105mg, 3.000eq.) was then added in one portion and the mixture was stirred vigorously at room temperature for 30 minutes. Saturated aqueous sodium hydrogen carbonate (0.5ml) was then added, followed by dichloromethane (10ml) and the mixture was stirred vigorously at room temperature for 10 min and the phases were separated (hydrophobic frit). The organic phase was evaporated under reduced pressure and the crude product was purified by column chromatography on silica (eluted with 0-12% (2M NH3 in MeOH) in DCM). Appropriate fractions were combined and evaporated under reduced pressure to give the free base of the title compound as a pale yellow solid (65mg). MS (ES+)m/z 481(MH+). 1H NMR (CDCI3): δ 8.12 (1H, s);7.82 (1H, s); 7.76 (1H, d, J= 9.7Hz); 7.17 (1H, s); 6.38 (1H, d, J = 9.7Hz); 5.03 (1H, m); 4.55 (1H, dd, J= 12.5Hz, 4.5Hz); 4.37 (3H, m); 3.79 (2H, s); 3.13 (3H, m); 2.94 (1H, m); 2.69 (2H, m); 2.52 (1H, m); 2.30 (4H, m); 1.86 (3H, m);1.37(2H,m). The free base (60mg, 0.125mmol) was suspended in dry DCM (1ml) and a 1M solution of hydrogen chloride in diethyl ether (125uL, l.OOOeq.) was added. The system was kept sealed and shaken for 1 minute then the solvents v/ere removed under reduced pressure and the residue was dried on the vacuum line to give the title compound as an amorphous yellow solid (64mg). MS (ES+) m/z4Sl(MH+). Example 28 (2R)-2-({4-[([l,3]Oxathio]o[4,5-c]pyridin-6-ylniethyl)ainino]-lpiperidinyl} methyl)-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione hydrochloride - 8 1 - N (a)[1,3]Oxathiolo[4,5-c]pyridine-6-carbaldehyde The title compound was prepared by: (i) treatment of [5-({[4- (methyloxy)phenyl]methyl}oxy)-4-oxo-l,4-dihydro-2-pyridinyl]methyl acetate (for a synthesis see WO2004058144, Example 60(c)) with triphenylphospine, diisopropylazodicarboxylate and benzyl alcohol to give {5-({[4- (methyloxy)phenyl]methyl}oxy)-4-[(phenylmethyl)oxy]-2-pyridinyl}methyl acetate; (ii) treatment of {5-({[4-(methyloxy)phenyl]methyl}oxy)-4-[(phenylmethyl)oxy]-2- pyridinyl} methyl acetate with trifluoroacetic acid and triethylsilane to give {5-hydroxy-4- [(phenylmethyl)oxy]-2-pyridinyl} methyl acetate trifluoroacetate; (iii) treatment of {5- hydroxy-4-[(phenylmethyl)oxy]-2-pyridinyl}methyl acetate trifluoroacetate with 1,1,1- trifluoro-N-phenyl-N-[(trifluoromemyl)sulfonyl]memanesulfonamideandrriethylamine to give (4-[(phenylmethyl)oxy]-5- {[(trifluoromethyl)sulfonyl]oxy} -2-pyridinyl)methyl acetate; (iv) treatment of (4-[(phenylmethyl)oxy]-5-{[(trifluoromethyl)sulfonyl]oxy}-2- pyridinyl)methyl acetate with (R)-(+)-2,2 bis(diphenylphosphino)-l,l-binaphthyl, palladium acetate and sodium 2-methyl-2-propanethiolate to give {5-[(l,ldimethylethyl) thio]-4-[(phenylmethyl)oxy]-2-pyridinyl}methyl acetate; (v) treatment of {5-[(l,l-dimethylethyl)thio]-4-[(phenylmethyl)oxy]-2-pyridinyl}methyl acetate with palladium on carbon under 1 atmosphere of hydrogen to give {5-[(1,1- dimethylethyl)thio]-4-oxo-l,4-dihydro-2-pyridinyl}methyl acetate; (vi) treatment of {5- [(1,1 -dimethylethyl)thio]-4-oxo-1,4-dihydro-2-pyridinyl}methyl acetate with concentrated hydrochloric acid to give 2-(hydroxymethyl)-5-mercapto-4(lH)-pyridinone; (vii) treatment of 2-(hydroxymethyl)-5-mercapto-4(lH)-pyridinone with potassium carbonate and dibromomethane to give [l,3]oxathiolo[4,5-c]pyridin-6-ylmethanol and (viii) treatment of [l,3]oxathiolo[4,5-c]pyridin-6-ylmethanol with manganese dioxide to give the title compound. (b) Title compound (2R)-2-[(4-Amino-1 -piperidinyl)methyl]-l ,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylene- 3,8-dione(50mg, 0.166mmol) (for a preparation see Example 16(j)) and [l,3]oxathiolo[4,5-c]pyridine-6-carbaldehyde (25mg, 0.901eq.) were stirred in 9:1 v:v chloroform:methanol (1ml) for 2.5 hours. Sodium triacetoxyborohydride (105mg, 3.000eq.) was then added in one portion and the mixture was stirred vigorously at room temperature for 30 min.Saturated aqueous sodium hydrogen carbonate (0.5ML) was then added, followed by dichloromethane (10ml) and the mixture was stirred vigorously at -82- room temperature for 10 min and the phases were separated (hydrophobic frit). The organic phase was evaporated under reduced pressure and the crude product was purified by column chromatography on silica (eluted with 0-12% (2M NH3 in MeOH) in DCM). Appropriate fractions were combined and evaporated under reduced pressure to give the free base of the title compound as a pale yellow amorphous solid (48mg). 1H NMR (CDCI3) δ 8.22 (1H, s); 7.82 (1H, s); 7.76 (1H, d, J= 9.7Hz); 6.80 (1H, s); 6.38 (1H, d, J= 9.7Hz); 5.77 (2H, s); 5.03 (1H, m); 4.55 (1H, dd, J = 12.5Hz, 4.6Hz); 4.38 (1H, dd, J= 12.5Hz, 9.4Hz); 3.81 (2H, s); 3.13 (1H, dd, J= 13.0Hz, 3.5Hz); 2.93 (1H, m); 2.68 (2H, m); 2.49 (1H, m); 2.33 (1H, dt, J= 11.4Hz, 2.6Hz); 2.24 (1H, dt, J= 11.4Hz, 2.6Hz); 1.84 (3H, m); (NH under HOD peak at 1.66); 1.34 (2H, m). MS(ES+)m/z453(MH+). The free base of the title compound (48mg, 0.106mrnol) was suspended in dry DCM (1ml) and a 1M solution of hydrogen chloride in diethyl ether (106uL, l.OOOeq.) was added. The system was kept sealed and shaken for 1 minute then the solvents were removed under reduced pressure and the residue was dried on the vacuum line to give the title compound as an amorphous yellow solid (48mg). MS (ES+) m/z 453(MH+). Example 29 (2R)-2-[(4-{[(3-Oxo-3,4-dihydro-2H-pyrido [3,2-6] [l,4]thiazin-6- yl)methyl]amino}-1-piperidinyl)methyl]-1,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylene- 3,8-dione hydrochloride H XXXJ N (2R)-2-[(4-Amino-l-piperidinyl)methyl]-l,2-dihydro-3H,8H-2a,5,8atriazaacenaphthyIene- 3,8-dione (50mg, 0.166mmol) (for a preparation see Example 16(j)) and 3-oxo-3,4-dihydro-2H-pyrido[3,2-b][l,4]thiazine-6-carboxaldehyde (29mg, 0.90eq.) (for a synthesis see WO2003087098, Example 301(d)) were stirred in 9:1 chlorofornv.methanol (1ml) at room temperature for 3 hours, then sodium triacetoxyborohydride (105mg, 3.00eq.) was added. The mixture was stirred at room temperature for a further 30 minutes, then saturated aqueous sodium hydrogen carbonate (0.5ml) was added, and the organic phase was diluted with DCM (10ml). The mixture was stirred vigorously for 10 minutes, then the organic phase was separated (hydrophobic frit) and evaporated under reduced pressure. The residue was taken up in DCM (ca. 3ml) + 1 drop MeOH and purified by column chromatography on silica (eluted with 0-12% - 8 3 - (2M NH-j in MeOH) in DCM). Appropriate fractions were combined and evaporated to give the free base of the title compound as a yellow amorphous solid. lH NMR (CDCl3) δ 8.58 (1H, broad s); 7.83 (1H, s); 7.77 (1H, d,J= 9.7Hz); 7.57 (1H, d, J = 7.8Hz); 6.97 (1H, d, J = 7.8Hz); 6.38 (1H, d, J= 9.7Hz); 5.04 (1H, m); 4.55 (1H, dd, J= 12.5Hz, 4.5Hz); 4.38 (1H, dd, J= 12.5Hz, 9.3Hz); 3.82 (2H, s); 3.47 (2H, s): 3.14 (1H, dd, J= 13.0Hz, 3.5Hz); 2.94 (1H, m); 2.69 (2H, m); 2.51 (1H, m); 2.33 (1H, dt,J = 11.4Hz, 2.4Hz); 2.25 (1H, dt, J= 11.4Hz, 2.4Hz); (NH under HOD peak at 2.06); 1.85 (2H,m);1.37(2H,m). MS (ES+) m/z 480(MH+). The free base of the title compound (43mg, 0.090mmol) was dissolved in DCM (2ml) and a IM solution of hydrogen chloride in diethyl ether was added. The system was kept sealed and shaken for 1 minute then the solvents were removed under reduced pressure and the residue was dried on the vacuum line to give the title compound as an amorphous yellow solid (38mg). MS (ES+) m/z 480(MH+). The solvents were removed and the solid dried in the desiccator (P2O5) overnight to afford the product as a white solid (5 lmg, LCMS and NMR consistent with product). Example 30 (1R)-l-({4-[(2,3-Dihydro-l,4-benzodioxin-6-ylmethy])amino]-lpiperidinyl} methyl)-1,2-dihydro-4H,9H-imidazo[1,2,3-ij]-1,8-naphthyridine-4,9- dione hydrochloride A suspension of (1R)-1 -[(4-amino-1 -piperidinyl)methyl]-1,2-dihydro-4H,9Himidazo[ l,2,3-ij]-l,8-naphthyridine-4,9-dione dihydrochloride (for a preparation see Example 5A(j)) (60mg, 0.161 mmol) in chloroform (2 ml) and methanol (0.100 ml) at rt under argon was treated with triethylamine (0.067 ml, 0.482 mmol) and stirred at rt for 15h. The solution was then treated with 2,3-dihydro-l,4-benzodioxin-6-carbaldehyde (commercially available)(23.75 mg, 0.145 mmol) and stirred for a further 30min. The solution was then treated with sodium triacetoxyborohydride (102 mg, 0.482 mmol) and stirred at rt for 30min, LC-MS after 30min showed some starting material present, more sodium triacetoxyborohydride (19mg) was added, the reaction was stirred for 15min. This was then treated with saturated aqueous NaHCO3 (10ml) and extracted with 20% methanol/DCM (3 x 25ml). The combined organic extracts were dried (MgSO), filtered, evaporated and chromatographed (0-20% methanol/DCM) to give the title compound as the free base (48mg, 67%) as a yellow gum. 1H NMR δH CDCl3, (250MHz) 1.28-1.51 (m, 2H),1.75-1.99 (m, 2H), 2.13-2.38 (m, 2H), 2.41-2.80 (m, 3H), 2.90-3.15 (m, 2H), -84- 3.75 (s, 2H), 4.22 (s, 4H), 4.31-4.42(m, 1H), 4.51-4.62 (m, 1H), 4.90-5.08 (m, 1H), 6.20- 6.32 (m, 2H), 6.81 (m, 2H), 6.84 (m, 1H), 7.42-7.53 (m, 2H). MS (ES+) m/z 449 (MH+). The free base of the title compound was dissolved in a small amount of DCM, treated with one equivalent of 1M HC1 in diethyl ether and then evaporated to give the title compound as the mono-HCl salt (44mg, 53%). LCMS was consistent with product. Example 31 (1R)-l-[(4-{[(8-Fluoro-2,3-dihydro-l,4-benzodioxin-6- yl)methyl]amino}-l-piperidinyl)methyl]-l,2-dihydro-4H,9H-imidazo[l,2,3-ij]-1,8- naphthyridine-4,9-dione hydrochloride A suspension of (1R)-l-({4-[(l,2,3-benzothiadiazol-5-ylmethyl)amino]-lpiperidinyl} methyl)-1,2-dihydro-4H,9H-imidazo [ 1,2,3-ij]-1,8-naphthyridine-4,9-dione dihydrochoride (for a preparation see Example 5A(j)) (50mg, 0.134 mmol) in chloroform (2 ml) and methanol (0.100 ml) at rt under argon was treated with triethylamine (0.056 ml, 0.402 mmol) and stirred at rt for 15min. The solution was then treated with 8-fluoro- 2,3-dihydro-l,4-benzodioxin-6-carbaldehyde (for a synthesis see WO2007122258, Example 8(b)) (21.96 mg, 0.121 mmol) and stirred for a further 30min. The solution was treated with sodium triacetoxyborohydride (85 mg, 0.402 mmol) and stirred at rt for 30min, LCMS after 30min showed there was still starting material and the imine of the product. So more sodium triacetoxyborohydride (40 mg) was added, the reaction was stirred for a further 30min. LCMS after this time showed that the reaction was complete. The reaction was then treated with saturated aqueous NaHC03 (10ml) and extracted with 20% methanoI/DCM (3 x 25ml). The combined organic fractions were dried (MgSCU), filtered, evaporated and chromatographed (0-20% methanol/DCM) to give the free base of the title compound (6mg, 9.6%) as a pale yellow solid and some crude product (15mg, 24%) as an impure pale yellow solid which was purified using an SCX column to give more identical title compund, free base. 1HNMR 5H CDCl3, (250MHz) 1.15-1.50 (m, 2H),1.70-2.10 (m, 2H), 2.15-2.39 (m, 2H), 2.41-2.58 (m, 1H), 2.60-2.74 (2H, m), 2.85-3.11 (m, 1H), 3.11-3.15 (m, 1H), 3.69 (s, 2H), 4.22-4.45(m, 5H), 4.50-4.62 (m, 1H), 4.90-5.09 (m, 1H), 6.20-6.35 (m, 2H), 6.60-6.72 (m, 2H), 7.41-7.52 (m, 2H). MS (ES+) m/z 467 (MH+). The free base of the title compound was then treated with one equivalent of 1M HC1 in diethyl ether to give the title compound as the mono hydrochloride salt (16.7mg, 27.5%). LCMS was consistent with product. -85- Example 32 (1R)-l-[(4-{[(7-Ch!oro-3-oxo-3,4-dihydro-2iy-pyrido[3,2-6][l,4]oxazin- 6-yl)methyl]amino}-l-piperidinyl)methyl]-l,2-dihydro-4H,9H-imidazo[l,2,3-ij]-1,8- naphthyridine-4,9-dione dihydrochloride / \ H •JO In a 10 mL round-bottomed flask (1R)-l-[(4-amino-l-piperidinyl)methyl]-l,2- dihydro-4H,9H-imiazo[l,2,3-ij]-l,8-naphthyridine-4,9-dione (for a preparation see Example 5A(j) (80 mg, 0.266 mmol), 7-chloro-3-oxo-3,4-dihydro-2H-pyrido[3,2- b][l,4]oxazine-6-carbaldehyde (for a synthesis see WO2003064421, Example 15(c)) (62.3 mg, 0.293 mmol), and sodium bicarbonate (100 mg, 1.190 mmol) in DCM (4 ml) and methanol (1 ml) were combined to give a brown solution. Sodium sulfate (200 mg, 1.408 mmol) was added and the reaction was allowed to stir at rt overnight. After 15h sodium triacetoxyborohydride (113 mg, 0.533 mmol) was added and the reaction was allowed to stir at 25 °C under nitrogen for 4h. The reaction mixture was adsorbed onto silica and purified using 0-10% MeOH/DCM (1% NH4OH). The LCMS and 1H NMR of the product were consistent with the title compound as the free base. 1HNMR 6H D-4 MeOH, (400MHz) 1.24-1.45 (m, 2H),1.79-1.96 (m, 2H), 2.22-2.31 (m, 2H), 2.46-2.53 (m, 1H), 2.59-2.68 (m,lH), 2.87-3.09 (m, 4H), 3.89 (s, 2H), 4.42-4.51 (m, 2H), 4.69 (s, 2H), 5.07-5.15(m, 1H), 6.26-6.35 (m, 2H), 7.39 (s,lH), 7.75-7.81 (m,2H), MS (ES+) m/z 497/499 (MH+). The free base of the title compound was taken up in 10% MeOH/DCM and treated with IN HC1 to form title compound as the diHCl salt (55 mg, 36.2%) Example 33 (1R)-l-[(4-{[(4-Chloro-7-oxo-6,7-dihydro-1H-pyrimido[5,4- 6][l,4]oxazin-2-yl)methyl]amino}-l-piperidinyl)methyl]-l,2-dihydro-4H,9Himidazo[ l,2,3-ij]-l,8-naphthyridine-4,9-dione In a 10 mL round-bottomed flask were combined (1R)-l-[(4-amino-lpiperidinyl) methyl]-l,2-dihydro-4H,9H-imidazo[l,2,3-ij]-l,8-naphthyridine-4,9-dione (for a preparation see Example 5A(j)) (60 mg, 0.178 mmol), 4-chloro-7-oxo-6,7-dihydrolH- pyrimido[5,4-b][l,4]oxazine-2-carbaldehyde (for a synthesis see WO2008009700, Example 124(g)) (38mg, 0.178 mmol), and sodium bicarbonate (150 mg, 1.78 mmol) in DCM(5 ml) and methanol (1 ml) to give a brown solution. Sodium sulfate (200 mg, 1.408 -86- mmol) was added and the reaction was allowed to stir at it overnight. After 15h sodium triacetoxyborohydride (113 mg, 0.533 mmol) was added and the reaction was allowed to stir at 25 °C under nitrogen for 4h. The reaction mixture was adsorbed onto silica and purified using 0-20% MeOH/DCM (1% NH4OH) to give the title compound (4mg orange solid, 4.51%). 1HNMR 8H CDC13, (250MHz) 1.28-1.51 (m, 2H),1.75-1.99 (m, 2H), 2.13-2.38 (m, 2H), 2.41-2.80 (m, 3H), 2.90-3.15 (m, 2H), 3.75 (s, 2H), 4.31-4.42(m, 1H), 4.51-4.62 (m, 1H), 4.8 (s, 2H), 4.90-5.08 (m, 1H), 6.25-6.32 (m, 2H), 7.51-7.53 (m, 2H), MS (ES+) m/z 498/500 (MH+). Example 34 (1R)-l-[(4-{[(7-Oxo-6,7-dihydro-1H-pyrimido[5,4-6) [l,4]thiazin-2- yl)methyl]amino}-l-piperidinyl)methyl]-l,2-dihydro-4H,9H-imidazo[1,2,3-ij]-1,8- naphthyridine-4,9-dionedihydrochloride r-rOsYrY .0 (a) Ethyl [(2,4-dioxo-l,2,3,4-tetrahydro-5-pyrimidinyl)thio]acetate A solution of 5-bromo-2,4(1H,3H)-pyrimidinedione (15 g, 79 mmol) and ethyl mercaptoacetate (8.58 ml, 79 mmol) in DMF (200mL) was treated with tetrabutylaminonium hydrogen sulfate (6.67 g, 19.64 mmol) and potassium carbonate (23.88 g, 173 mmol) and stirred at ambient temperature overnight. The solution was filtered and concentrated under reduced pressure to yield crude title compound as a yellow oil which foams up under reduced pressure. MS(ES+)m/z231.1(MH+). (b) Ethyl [(2,4-dichloro-5-pyrimidinyl)thio]acetate A suspension of ethyl [(2,4-dioxol, 2,3,4-tetrahydro-5-pyrimidinyl)thio]acetate (crude material) (18.19 g, 79 mmol) in phosphorus oxychloride (100 ml, 1073 mmol) was treated with dimethyl aniline (2.500 ml, 19.72 mmol), and the reaction was heated to reflux and stirred for 2 hours. The solution was allowed to cool to room temperature and poured slowly onto ice to quench the excess phosphorus oxychloride. Once quenched, the aqueous layer was extracted with CH2CI2 (3X). The organic layers were combined, dried over Na2S04, filtered, and concentrated under reduced pressure. The crude material was chromatographed using a gradient of 0-50% EtOAc/Hexanes. The product was isolated as a dark yellow oil. 1H NMR (400 MHz, chloroform-d) ppm 1.22 (t, J=7.07 Hz, 3 H) 3.71 (s, 2 H) 4.15 (d, J=7.33 Hz, 1 H) 8.53 (s, 1 H) (c) Ethyl [(4-amino-2-chloro-5-pyrimidinyl)thio]acetate A solution of ethyl [(2,4-dichloro-5-pyrimidinyl)thio]acetate (2.0 g, 7.49 mmol) in DMF (75ml) was treated with aminonia in isopropanol (7.49 ml, 14.97 mmol) in a -87- pressure tube. The tube was capped, and the reaction was stirred at ambient temperature. Upon completion, the solution was concentrated under reduced pressure and pumped on to remove any residual DMF. The crude material was chromatographed using a gradient of 0-10% acetone/chloroform. The product contained a small amount of cyclized material (which is the product of the next step). The product was isolated as a light yellow solid. MS (ES+) m/z 248.0 (MH+). (d) 2-Chloro-1H-pyrimido[5,4-b][l,4]thiazin-7(6H)-one A suspension of ethyl [(4-amino-2-chloro-5-pyrimidinyl)thio]acetate (0.786 g, 3.17 mmol) in ethanol (50 ml) was heated to 70°C. Cesium carbonate (1.034 g, 3.17 mmol) was added and the solution was heated for a further 5 minutes. A white solid precipitated out of solution almost immediately. The solution was concentrated under reduced pressure. The residue was dissolved in water and brought to pH = 5 with IN HC1. The aqueous layer was extracted with CH2CI2 (2X). The organic layers were combined, dried over Na2SO4, filtered, and concentrated under reduced pressure to yield a light yellow solid. MS (ES+) m/z 202.0 (MH+). (e) 2-Ethenyl-1 H-pyrimido[5,4-b][ 1,4]thiazin-7(6H)-one 2-Chloro-lH-pyrimido[5,4-b][l,4]thiazin-7(6H)-one (0.639 g, 3.17 mmol) was treated with tributylvinyl tin (1.388 ml, 4.76 mmol), and tetrakis(triphenylphosphine) palladium(O) (0.293 g, 0.254 mmol) in 1,4-dioxane (4 ml) and toluene (4 mL) in a microwave vial. The reaction was heated in the microwave at 140°C for 20 minutes. The solution was diluted with EtOAc and washed with saturated NaHCO3 solution. The aqueous layer was extracted with EtOAc (2X). The organic solution were combined, dried over Na2SO4, filtered, and concentrated under reduced pressure. The crude material was chromatographed using a gradient of 0-60% CH2Cl2/(CH2Cl2/MeOH/NH4OH) (90:10:1). The product was isolated as a mixture of the desired product and triphenylphosphine. Pure material was obtained by triturating and washing with diethyl ether. The product was isolated as an orange solid. MS (ES+) m/z 194.0 (MH+). (f) 7-Oxo-6,7-dihydro-lH-pyrimido[5,4-b][l,4]thiazine-2-carbaldehyde A solution of 2- ethenyl-lH-pyrimido[5,4-b][l,4]thiazin-7(6H)-one (0.262 g, 1.356 mmol) in methanol/DCM was cooled to -78°C and treated with ozone until the solution turned blue. The solution was stirred at -78°C for an additional 5 minutes. Dimethyl sulfide (5.0 ml, 67.6 mmol) was added and the solution was allowed to warm to room temperature and stir overnight. The solution was concentrated onto silical gel and the crude material was chromatographed using a gradient of 0-100% CH2Cl2/(CH2Cl2/MeOH/NH40H) (90:10:1). The product was isolated as a light yellow solid. MS (ES+) m/z 195.9 (MH+). (g) Title compound -88- A suspension of (1R)-l-[(4-amino-l-piperidinyl)methyl]-l,2-dihydro-4H,9Himidazo[ l,2,3-ij]-l,8-naphthyridine-4,9-dione (for a preparation see Example 5A(j)) (0.060 g, 0.179 mmol) in 1:1 CH2Cl2/MeOH (lOmL) was treated with 7-oxo-6,7-dihydrolH- pyrimido[5,4-b][l,4]thiazine-2-carbaldehyde (0.035 g, 0.179 mmol) and sodium bicarbonate (0.151 g, 1.793 mmol). Excess Na2SO4 was added and the reaction stirred at room temperature for 18 hours. Sodium triacetoxyborohydride (0.114 g, 0.538 mmol) was added and the reaction stirred at room temperature for 1 hour. The solution was concentrated onto silica gel and the crude material was chromatographed using a gradient of 0-100% CH2Cl2/(CH2Cl2/MeOH/NH4OH) (90:10:1). The free base of the title compound was isolated as a yellow solid (0.027g). MS (ES+) m/z 480.1 (MH+). 1HNMR (400 MHz, CHLOROFORM-d) ppm 1.62 (d, 7=2.53 Hz, 1 H) 1.61 (br. s., 1 H) 1.90 - 2.09 (m, 3 H) 2.20 - 2.42 (m, 2 H) 2.59 - 2.78 (m, 2 H) 3.14 (dd, 7=12.88, 3.03 Hz, 2 H) 3.53 (s, 2 H) 4.05 - 4.14 (m, 2 H) 4.41 (dd, 7=12.38, 9.35 Hz, 1 H) 4.57 (dd, 7=12.63,4.04 Hz, 1 H) 5.04 (dd, 7=7.96,4.42 Hz, 1 H) 5.32 (s, 1 H) 6.28 (dd, 7=16.29, 9.22 Hz, 2 H) 7.49 (d, 7=3.28 Hz, 1 H) 7.50 - 7.57 (m, 1 H). The title di-HCl salt was formed by dissolving the free base in CH2Cl2 and adding 0.113mL 1NHCl/ether. Example 35 (1H)-1-({4-[(l,2,3-BenzothiadiazoI-5-ylmethy])amino]-lpiperidinyl} methyl)-1,2-dihydro-4H,9H-imidazo[1,2,3-ij]-1,8-naphthyridine-4,9- dione hydrochloride A suspension of (1R)-1 -({4-[(l,2,3-benzothiadiazol-5-ylmethyl)amino]-1 - piperidinyl}methyl)-l,2-dihydro-4H,9H-imidazo[l,2,3-ij]-l,8-naphthyridine-4,9-dione dihydrochoride (for a preparation see Example 5A(j)) (60mg, 0.161 mmol) in chloroform (2 ml) and methanol (0.100 ml) at rt under nitrogen was treated with triethylamine (0.067 ml, 0.482 mmol) and stirred at rt for 15h. The solution was then treated with 1,2,3- benzothiadiazole-5-carbaldehyde (for a synthesis see WO0208224 Example 20(a)) (23.75 mg, 0.145 mmol) and stirred for a further 30min. The solution was then treated with sodium triacetoxyborohydride (102 mg, 0.482 mmol) and stirred at rt for 45min, LC-MS after 45min showed reaction complete. This was then treated with saturated aqueous NaHC03 (10ml) and extracted with 20% methanol/DCM (3 x 25ml). The combined -89- organic extracts were dried (MgSO4), filtered, evaporated and chromatographed (0-5% methanol/DCM 5%methanol/DCM) to give the free base of the title compound (26mg, 36%) as a pale yellow solid. 1HNMR 5H CDCl3, (400MHz) 1.30-1.49 (m, 2H),1.80-1.98 (m, 2H), 2.21-2.39 (m, 2H), 2.51-2.61 (m, 1H), 2.61-2.75 (m, 2H), 2.90-3.02 (m, 1H), 3.05-3.19 (m, 1H), 4.04 (s, 2H), 4.31-4.42(m, 1H), 4.51-4.61 (m, 1H), 4.92-5.05 (m, 1H), 6.20-6.31 (m, 2H), 7.45-7.53 (2H, m), 7.71 (d, 1H). 8.11 (d, 1H), 8.56 (s, 1H) MS (ES+) m/z 449 (MH+). The free base of the title compound was dissolved in a small amount of DCM, treated with one equivalent of 1M HC1 in diethyl ether and then evaporated to give the title compound as the mono-HCl salt (16.2mg, 20.8%). LCMS consistent with product. Example 36 (1R)-l-({4-[(2,3-Dihydro-l-benzofuran-5-ylmethyl)amino]-lpiperidinyI} methyl)-l,2-dihydro-4H,9H-imidazo[1,2,3-ij]-1,8-naphthyri(Iine-4,9- dione hydrochloride A suspension of (1R)-1 -({4-[(l ,2,3-benzothiadiazol-5-ylmethyl)amino]-1 - piperidinyl} methyl)-1,2-dihydro-4H,9H-imidazo[ 1,2,3 -ij] -1,8-naphthyridine-4,9-dione dihydrochoride(for a preparation see Example 5A(j)) (60mg, 0.161 mmol) in chloroform (2 ml) and methanol (0.100 ml) at rt under nitrogen was treated with triethylamine (0.067 ml, 0.482 mmol) and stirred at rt for 15min. The solution was then treated with 2,3- dihydro-l-benzofuran-5-carbaldehyde (commercially available) (0.020 ml, 0.161 mmol) and stirred for a further 30min. The solution was then treated with sodium triacetoxyborohydride (102 mg, 0.482 mmol) and stirred at rt for 45min. This was then treated with saturated aqueous NaHCO3 (10ml) and extracted with 20% methanol/DCM (3 x 25ml). The combined organic fractions were dried (NaSO4), filtered, evaporated and chromatographed (5-25% methanol/DCM) to give the free base of the title compound (24mg, 34.5%) as a white solid. 1HNMR5H CDCI3, (400MHz) 1.22-1.49 (m, 2H),1.79-2.10 (m, 2H), 2.21-2.40 (m, 2H), 2.45-2.58 (m, 1H), 2.61-2.72 (m, 2H), 2.90-3.01 (m, 1H), 3.05-3.15 (m, 1H), 3.21 (t, 2H), 3.72 (s, 2H), 4.32-4.42(m, 1H), 4.51-4.62 (m, 3H), 4.95-5.05 (m, 1H), 6.22-6.33 (m, 2H), 6.72(d, 1H), 7.14 (d, 1H), 7.19 (s, 1H), 7.45-7.52 (2H, m), MS (ES+) m/z 433 (MH+). -90- The free base of the title compound was dissolved in a small amount of DCM, treated with one equivalent of 1M HC1 in diethyl ether and then evaporated to give the title compound as the mono-HCl salt (22.7mg, 28.6%). LCMS consistent with product. Example 37 (1R)-1-({4-[(3,4-Dihydro-2H-pyrano[2,3-c]pyridin-6-ylmethyl)amino]- l-piperidinyl}methyl)-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione hydrochloride A suspension of (1R)-1-[(4-amino-1 -piperidinyl)methyl]-l,2-dihydro-3H,8H- 2a,5,8a-triazaacenaphthylene-3,8-dione dihydrochloride (for a preparation see Example 13(k) or 15(d)) (50 mg, 0.100 mmol) in chloroform (4 ml) and methanol (0.200 ml) at room temperature under nitrogen was treated with triethylamine (0.042 ml, 0.301 mmol) and stirred for 0.25h (the suspension turned into a solution). 3,4-Dihydro-2H-pyrano[2,3- c]pyridine-6-carbaldehyde (for a synthesis see WO2004058144, Example 126(e)) (16.35 mg, 0.100 mmol) was then added and the reaction was stirred at room temperature for 0.5h. Sodium triacetoxyborohydride (67.1 mg, 0.301 mmol) was then added and the reaction was stirred at room temperature. After 3h there was still some starting material so 30 mg of sodium triacetoxyborohydride were added. After lh a saturated aqueous solution of sodium bicarbonate (25mL) was added followed by 20% methanol/DCM (25mL) and the aqueous layer was extracted and then separated from the organic layer. The aqueous layer was extracted again twice with 20% methanol/DCM (2x25mL). The combined organiclayers were dried on sodium sulphate, filtered and evaporated to afford 60mg of crude product. The crude product was purified by silica chromatography (0- 20%MeOH/DCM) to afford the free base of the title compound as a yellow solid (39mg, 87%). 1HNMR δH CDC13, (400MHz) 1.25-1.45 (m, 2H), 1.78-2.08 (m, 4H), 2.22-2.38 (m, 2H), 2.45-2.60 (m, 1H), 2.62 (d, 1H), 2.67-2.80 (m, 3H), 2.93 (d, 1H), 3.05-3.14 (m, 1H), 3.78 (s, 2H), 4.15-4.25 (m, 2H), 4.30-4.45 (m, 1H), 4.50-4.60 (m, 1H), 4.95-5.05 (m, 1H), 6.33 (d, 1H), 6.96 (s, 1H), 7.75(s, 1H), 7.87 (s, 1H), 8.07 (s, 1H). MS (ES+) m/z 449 (MH+). The free base of the title compound was dissolved in a small amount of methanol/DCM and treated with leq of 1M hydrochloric acid in diethyl ether. The solvents were removed and the solid was dried in the desiccator (in the presence of P2O5) over the weekend to afford the title compound as the mono-HCl salt as a yellow solid (40.6mg, 79%). LCMS was consistent with product. - 9 1 - Example 38 (1R)-1-({4-[(23-Dihydrofuro[2y3-c]pyridin-5-ylmethyl)amino]-lpiperidinyl} methyl)-1,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione hydrochloride A suspension of (1R)-l-[(4-amino-l-piperidinyl)methyl]-l,2-dihydro-3H,8H- 2a,5,8a-triazaacenaphthylene-3,8-dione dihydrochloride (for a preparation see Example 13(k) or 15(d)) (50 mg, 0.100 mmol) in chloroform (20 ml) and methanol (0.800 ml) at room temperature under nitrogen was treated with triethylamine (0.042 ml, 0.301 mmol) and stirred for 0.25h (the suspension turned into a solution). 2,3-Dihydrofuro[2,3- c]pyridine-5-carbaldehyde (for a synthesis see WO2007122258, Example 43(f)(14.94 mg, 0.100 mmol) was then added and the reaction was stirred at room temperature for 0.5h. Sodium triacetoxyborohydride (67.1 mg, 0.301 mmol) was then added and the reaction was stirred at room temperature. After 3h there was still some starting material so 30 mg of sodium triacetoxyborohydride were added. After lh a saturated aqueous solution of sodium bicarbonate (25mL) was added followed by 20% methanol/DCM (25mL) and the aqueous layer was extracted and then separated from the organic layer. The aqueous layer was extracted again twice with 20% methanol/DCM (2x25mL). The combined organic extracts were dried on sodium sulphate, filtered and evaporated to afford 50mg of crude product. The crude product was purified by silica chromatography (0-20% methanol/DCM) to afford the free base of the title compound as a yellow solid (31mg,71.2%). lH NMR δH CDC13, (250MHz) 1.25-1.43 (m, 2H), 1.81-2.00 (m, 2H), 2.22-2.35(m, 2H), 2.49-2.54 (m, 1H), 2.66 (d, 1H), 2.71-2.74 (m, 1H), 2.92 (d, 1H), 3.07-3.12 (m, 1H), 3.19-3.24 (m, 2H), 3.82 (s, 2H), 4.37-4.42 (m, 1H), 4.56-4.62 (m, 3H), 4.96-5.06 (m, 1H), 6.33 (d, 1H), 7.18 (s, 1H), 7.76 (d, 1H), 7.86 (s, 1H), 8.06 (s, 1H). MS(ES+)m/z435(MH+). The free base of the title compound was dissolved in a small amount of methanol/DCM and treated with leq of 1M hydrochloric acid in diethyl ether. The solvents were removed and the solid was dried in the desiccator (in the presence of P2O5) over the weekend to afford the title compound as the mono-HCl salt as an orange solid (33.5mg, 67.4%). LCMS was consistent with product. Example 39 (2R)-2-({4-[(3,4-Dihydro-2H-pyrano[2,3-c]pyridin-6-yImethyl)amino]- l-piperidinyl}methyl)-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione hydrochloride -92- A suspension of (2i?)-2-[(4-amino-1 -piperidinyi)methyl]-l ,2-dihydro-3H,8H- 2a,5,8a-triazaacenaphthylene-3,8-dione (for a preparation see Example 16A(j)) (60mg, 0.199 mmol) and 3,4-dihydro-2H-pyrano[2,3-c]pyridine-6-carbaldehyde (for a synthesis see WO2004058144, Example 126(e)) (29.2 mg, 0.179 mmol) in chloroform (2 ml) and methanol (0.061 ml) at rt under nitrogen was treated with sodium triacetoxyborohydride (127 mg, 0.597 mmol) and stirred at rt for 30min. The reaction was then treated with saturated aqueous NaHCCh (10ml) and extracted with 20% methanol/DCM (3 x 20ml). The combined organic extracts were dried (MgS04), filtered, evaporated and purified using silica chromatography (0-20% MeOH/DCM) to give the free base of the title compound as a light brown solid 1 H N M R H: CDC13, (400MHz) 1.28-1.40 (m, 2H), 1.78-1.86 (m, 2H), 1.96-2.01 (m, 2H), 2.14-2.34 (m, 2H), 2.45-2.52 (m, 1H), 2.62-2.74 (m, 4H), 2.91 (m, 1H), 3.07-3.11 (m, 1H), 3.74 (s, 2H), 4.16-4.18 (m, 2H), 4.32-4.37 (m, 1H), 4.48-4.52 (m, 1H), 4.97-5.03 (m, 1H), 6.34 (d, 1H), 6.93 (s, 1H), 7.72 (d, 1H), 7.77 (s, 1H), 8.03 (s, 1H), MS (ES+) m/z 449 (MH+). The free base of the title compound in a small amount of DCM was treated with one equivalent of IM HCl in diethyl ether (0.17ml), evaporated and dried in a dessicator overnight to give the title compound as the mono-HCl salt (57.3 mg, 56.4 % yield). LCMS consistent with product. Example 40 (2R)-2-({4-[(2,3-Dihydro-l,4-benzodioxin-6-ylmethyl)amino]-1- piperidiny]}methyl)-1,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione hydrochloride A suspension of (2R)-2-[(4-amino-1-piperidinyl)methyl]-l,2-dihydro-3H,8H- 2a,5,8a-triazaacenaphthylene-3,8-dione (for a preparation see Example 16A(j)) (60mg, 0.199 mmol) and 2,3-dihydro-l,4-benzodioxin-6-carbaldehyde (commercially available) - 9 3 - (29.4 mg, 0.179 mmol) in chloroform (2 ml) and methanol (0.100 ml) at rt under nitrogen was treated with sodium triacetoxyborohydride (127 mg, 0.597 mmol) and stirred for 90min, LC-MS after 90min showed reaction complete. This was treated with saturated aqueous NaHC03 (10ml) and extracted with 20% methanol/DCM (3 x 25ml). The combined organic extracts were dried (MgSO4), filtered, evaporated and chromatographed (0-20% methanol/DCM) to give the free base of the title compound (46.8 mg, 58.4%) as a yellow gum. 1H NMR δH CDC13, (400MHz) 1.20-1.41 (m, 2H),1.73-1.91 (m, 2H), 2.09-2.38 (m, 2H), 2.42-2.55 (m, 1H), 2.61-2.72 (m, 2H), 2.85-2.95 (m, 1H), 3.05-3.15 (m, 1H), 3.68 (s, 2H), 4.32 (m, 4H). 4.32-4.42 (m, 1H), 4.49-4.59 (m, 1H), 4.95-5.06 (m, 1H), 6.39 (d, 1H), 6.71-6.85 (m, 3H), 7.75 (d, 1H), 7.81 s, 1H). MS (ES+) m/z 450 (MH+). The free base of the title compound was dissolved in a small amount of DCM, treated with one equivalent of 1M HCl in diethyl ether and then evaporated to give the title compound as the mono-HCl salt (43.8mg, 53.4%). LCMS consistent with product. Example 41 (2R)-2-[(4-{[(8-Fluoro-2,3-dihydro-l,4-benzodioxin-6- yl)methyl]amino}-l-piperidinyl)methyl]-1,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylene- 3,8-dione hydrochloride A suspension of (2R)-2-[(4-amino-l-piperidinyl)methyl]-1,2-dihydro-3H,8H- 2a,5,8a-triazaacenaphthylene-3,8-dione (for a preparation see Example 16A(j)) (58.5mg, 0.194 mmol) and 8-fluoro-2,3-dihydro-l,4-benzodioxin-6-carbaldehyde (for a synthesis see WO2007122258, Example 8(b)) (31.8 mg, 0.175 mmol) in chloroform (2 ml) and methanol (0.100 ml) at room temperature under nitrogen was stirred for 0.5h. This was then treated with sodium triacetoxyborohydride (123 mg, 0.582 mmol) and stirred for 90min. This was then treated with saturated aqueous NaHCO3 (10ml) and extracted with 20% methanol/DCM (3 x 25ml). The combined organic extracts were dried (NaSO4), filtered, evaporated and purified using silica chromatography (0-20% methanol/DCM) to give the free base of the title compound as a yellow gum (44.9 mg, 49.5%). 1HNMR δH CDC13, (400MHz) 1.24-1.55 (m, 2H), 1.78-1.85 (m, 2H), 2.21-2.36 (m, 2H), 2.44-2.51 (m, 1H), 2.64-2.73 (m, 2H), 2.92 (d, 1H), 3.10-3.14 (m, 1H), 3.65 (s, 2H), 4.26- 4.30 (m, 4H), 4.35-4.40 (m, 1H), 4.52-4.56 (m, 1H), 4.99-5.05 (m, 1H), 6.38 (d, 1H), 6.62-6.67 (m, 2H), 7.76 (d, 1H), 7.81 (s, 1H), MS (ES+) m/z 468 (MH+). The free base of the title compound was dissolved in a small amount of DCM, treated with one equivalent of 1M HCl in diethyl ether and then evaporated and dried in a -94- desiccator overnight to give the title compound as the mono-HCl salt (30.1mg, 29.2%). LCMS consistent with product. Example 42 7-{[(l-{[(2R)-3,8-Dioxo-l,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylen- 2-yl]methyl}-4-piperidinyl)amino]methyl}-2,3-dihydro-l,4- benzodioxin-5-carbonitrile hydrochloride A suspension of (2R)-2-[(4-amino-1 -piperidinyl)methyl]-1,2-dihydro-3H,8H- 2a,5,8a-triazaacenaphthylene-3,8-dione (for a preparation see Example 16A(j)) (60mg, 0.199 mmol) and 7-formyl-2,3-dihydro-l,4-henzodioxin-5-carbonitrile (for a synthesis see WO06014580 Preparation 13 or WO2007122258, Example 31(d)) (37.7 mg, 0.199 mmol) in chloroform (2 ml) and methanol (0.100 ml) at room temperature under nitrogen was treated with sodium triacetoxyborohydride (127 mg, 0.597 mmol) and stirred for 90min. This was then treated with saturated aqueous NaHCO3(10ml) and extracted with 20% methanol/DCM (3 x 25ml). The combined organic extracts were dried (NaS04), filtered, evaporated and purified using silica chromatography (0-20% methanol/DCM) to give the free base of the title compound as a yellow gum (40 mg, 42.3%). 1H NMR δH CDC13, (400MHz) 1.24-156 (m, 2H), 1.79-1.86 (m, 2H), 2.21-2.36 (m, 2H), 2.43-2.50 (m, 1H), 2.65-2.72 (m, 2H), 2.93 (d, 1H), 3.12-3.16 (m, 1H), 3.68 (s, 2H), 4.29- 4.41 (m, 5H), 4.53-4.57 (m, 1H), 5.00-5.06 (m, 1H), 6.39 (d, 1H), 7.06-7.09 (m, 2H), 7.77 (d, 1H), 7.83 (s, 1H), MS(ES+)m/z 475(MH+). The free base of the title compound was dissolved in a small amount of DCM, treated with one equivalent of 1M HC1 in diethyl ether, evaporated and dried in a dessicator overnight to give the title compound as the mono-HCl salt (45mg, 42%) as a pale yellow solid. LCMS consistent with product. Example 43 (2R)-2-({4-[(2,3-Dihydrofuro [2,3-c]pyridin-5-ylmethyl)amino]-1- piperidinyl}methyl)-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthyIene-3,8-dione hydrochloride 95- A suspension of (2R)-2-[(4-amino-l-piperidinyl)methyl]-l,2-dihydro-3H,8H- 2a,5,8a-triazaacenaphthylene-3,8-dione (for a preparation see Example 16A(j)) (70mg, 0.232 mmol) and 2,3-dihydrofuro[2,3-c]pyridine-5-carbaldehyde (for a synthesis see WO2007122258, Example 43(f) (34.6 mg, 0.232 mmol) in chloroform (5 ml) and methanol (0.250 ml) at room temperature under nitrogen was stirred for 0.5h (the suspension turned into a solution). Sodium triacetoxyborohydride (155 mg, 0.697 mmol) was then added and the reaction was stirred at room temperature. After 3h there was no starting material left so a saturated aqueous solution of sodium bicarbonate (25mL) was added followed by 20% methanol/DCM (25mL) and the aqueous layer was extracted and then separated from the organic layer. The aqueous layer was extracted again twice with 20% methanol/DCM (2x25mL). The combined organic extracts were dried on sodium sulphate, filtered and evaporated to afford 90mg of crude product.The crude product was purified by silica chromatography (0-20%MeOH/DCM) to afford the free base of the title compound as a pale yellow solid (77mg, 76%). 1H NMR δH CDC13, (400MHz) 1.28-1.48 (m, 2H), 1.81-1.89 (m, 2H), 2.21-2.36 (m, 2H), 2.43-2.55 (m, 1H), 2.64-2.72 (m, 2H), 2.93 (d, 1H), 3.10-3.14 (m, 1H), 3.19-3.23 (m, 2H), 3.81 (s, 2H), 4.34-4.40 (m, 1H), 4.52-4.61 (m, 3H), 4.95-5.08 (m, 1H), 6.37 (d, 1H), 7.17 (s, 1H), 7.76 (d, 1H), 7.81 (s, 1H), 8.06 (s, 1H). MS (ES+) m/z 435 (MH+). The free base of the title compound was dissolved in a small amount of methanol/DCM and treated with leq of 1M hydrochloric acid in diethyl ether. The solvents were removed and the solid was dried in the desiccator (in the presence of P2O5) over the weekend to afford the title compound as the mono-HCl salt as an off-white solid (78.9mg, 68.5%). LCMS was consistent with product. Example 44 (1R)-1-({4-[(2,3-Dihydro-l,4-benzodioxin-6-y]methyl)amino]-1- piperidinyl}methyl)-1,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione hydrochloride OO^/ N^ ^N A suspension of (1R)-1-[(4-amino-1-piperidinyl)methyl]-l,2-dihydro-3H,8H- 2a,5,8a-triazaacenaphthylene-3,8-dione dihydrochloride (for a preparation see Example 13(k) or 15(d)) (50 mg, 0.100 mmol) in chloroform (4 ml) and methanol (0.200 ml) at room temperature under nitrogen was treated with triethylamine (0.042 ml, 0.301 mmol) and stirred for 0.25h (the suspension turned into a solution). 2,3-Dihydro-l,4- benzodioxin-6-carbaldehyde (commercially available) (16.45 mg, 0.100 mmol) was then added and the reaction was stirred at room temperature for 0.5h. Sodium triacetoxyborohydride (67.1 mg, 0.301 mmol) was then added and the reaction was -96- stirred at room temperature. After 3h 40 mg more of sodium triacetoxyborohydride was added. After 1 h 30 mg more of sodium triacetoxyborohydride was added. After lh saturated NaHC03 (25mL) was added followed by 20% MeOH/DCM (25mL) and the aqueous layer was separated from the organic layer. The aqueous layer was extracted again twice with 20%MeOH/DCM (2x25mL). The combined organic extracts were dried NaSO4, filtered and evaporated to afford the crude product. The crude product was purified by chromatography on silica (0-20%MeOH/DCM) to give 27mg of the free base of the title compound (59.9% total yield). 1HNMR δH CDC13, (400MHz) 1.21-1.42 (m, 2H), 1.70-1.92 (m, 2H), 2.21-2.36 (m, 2H), 2.41-2.55 (m, 1H), 2.58-2.78 (m, 2H), 2.88-2.98 (m, 1H), 3.05-3.14 (m, 1H), 3.68 (s, 2H), 4.25(s, 4H), 4.43-4.52 (m, 1H), 4.51-4.62 (m, 1H), 4.98-5.06 (m, 1H), 6.34 (d, 1H), 6.75- 6.84 (m, 3H), 7.76 (d, 1H), 7.87 (s, 1H). MS (ES+) m/z 450 (MH+). The free base of the title compoundwas dissolved in a small amount of MeOH/DCM and treated with leq of a 1M solution of HC1 in Et20. The solvents were removed and the solid dried in the desiccator (P2O5) overnight to afford the title compound as the momo-HCl salt (26 mg, 0.051 mmol, 50.7 % yield) as a yellow solid. Example 45 (1H)-l-({4-[([l,2,5]Thiadiazolo[3,4-6]pyridin-6-ylmethyl)amino]-lpiperidinyl} methyl)-l,2-dihydro-4H,9H-imidazo[1,2,3-yl-1,8-naphthyridine-4,9- dione hydrochloride 0^VN vN^N \ £ ^ 0 ^XJ To(1R)-1-[(4-amino-l-piperidinyl}methyl)-l,2-dihydro-4H,9H-imidazo[l,2,3-ij]- l,8-naphthyridine-4,9-dione dihydrochoride (for a preparation see Example 5A(j) (60 mg, 0.161 mmol) was added chloroform (3 ml), methanol (0.3 ml) and triethylamine (0.067 ml, 0.482 mmol). The reaction was stirred under a nitrogen atmosphere for 30 mins, then [l,2,5]thiadiazolo[3,4-b]pyridine-6-carbaldehyde (for a preparation see Example 49(b)) (25.2 mg, 0.153 mmol) was added. The reaction was stirred for a further 2hrs then sodium triacetoxyborohydride (102 mg, 0.482 mmol) was added and stirring continued for 16 hours. Further sodium triacetoxyborohydride (102 mg, 0.482 mmol) was added -97- and stirred for 30mins. Further sodium triacetoxyborohydride (102 mg, 0.482 mmol) was added and stirring continued for 2 hours. The reaction was partitioned between sat. NaHCO3 and 20% MeOH in DCM. The aqueous was further extracted with 20% MeOH in DCM and the combined organic extracts passed thought a hydrophobic frit and concentrated to give a reddish brown solid (~65mg). This was purified by silica chromatography eluting with 0-20% MeOH in DCM to give the free base of the title compound as a pale tan gum (18mg). 1HNMR δH CD3OD 400MHz 1.30 (m, 1H), 1.42 (m, 1H), 1.88 (br d, 1H), 1.98 (br d, 1H), 2.28 (q, 2H), 2.63 (m, 2H), 2.89 (dd, 1H), 3.01 (dd, 1H), 3.06 (br d, 1H), 4.08 (s, 2H), 4.45 (m, 2H), 5.10 (m, 1H), 6.28 (dd, 2H), 7.76 (d, 2H), 8.41 (br d, 1H), 9.08 (br d, 1H) MS (ES+) m/z 450 (MH+). The free base of the title compound was dissolved in 2:1 DCM:MeOH (1ml) and HC1 (1M in diethyl ether) (0.040ml, 0.04mmol) was added. The solvent was evaporated to give a pale brown solid (22mg, 28%). Example 46 (1R)-1-[(4-{[(4-Fluoro-lH-benzimidazol-2-yl)methyl]amino}-lpiperidinyl) methyl]-1,2-dihydro-4H,9H-imidazo[l,2,3-ij]-1,8-naphthyridine-4,9- dione dihydrochloride To a 10 mL round-bottomed flask were added (1R)-l-[(4-amino-lpiperidinyl) methyl]-l,2-dihydro-4H,9H-imidazo[l,2,3-ij]-l,8-naphthyridine-4,9-dione (for a preparation see Example 5A(j)) (80 mg, 0.238 mmol), 4-fluoro-lH-benzimidazole- 2-carbaldehyde (for a synthesis see WO2003087098, Example 320) (42.9 mg, 0.261 mmol), and sodium bicarbonate (100 mg, 1.190 mmol) in DCM (4 ml) and methanol (1 ml) to give a brown suspension. Sodium sulfate (200 mg, 1.408 mmol) was added and the reaction was stirred at rt overnight. After 15h sodium triacetoxyborohydride (101 mg, 0.475 mmol) was added and the reaction was stirred at 25 °C under nitrogen for 4h. The reaction mixture was adsorbed onto silica and purified using 0 -10% MeOH/DCM (1% NH4OH) to give the free base of the title compound. The LCMS and 1H NMR were consistent with the desired product. 1H NMR δH D-4 MeOH, (400MHz) 1.35-1.55 (m, 2H),1.90-1.96 (m, 2H), 2.30-2.41 (m, 2H), 2.71-2.81 (m, 2H), 2.91-2.99 (m,lH), 3.05-3.15 (m, 2H), 4.20 (s, 2H), 4.41-4.50 (m, 2H), 4.69 (s, 2H), 5.10-5.20 (m, 1H), 6.25-6.36 (m, 2H), 6.91- 7.05 (m, 1H), 7.16-7.25 (m, 1H), 7.31-7.39 (m, 1H), 7.75-7.81 (m, 2H), MS (ES+) m/z 449 (MH+). -98- The free base of the title compound was taken up in 10% MeOH/DCM and treated with IN HC1 to form the title compound as the diHCl salt (17 mg, 0.033 mmol, 13.73 % yield). Example 47 (1R)-1-[((25)-2-{[([13]Oxathiolo[5,4-c]pyridin-6- ylmethyI)amino]methy]}-4-morpho]inyl)methyl]-l,2-dihydro-4H,9H-imidazo[1,2,3- ij]-1,8-naphthyridine-4,9-dionedihydrochloride > O^ M^ M^ sO (a) 1,1 -Dimethylethyl [((2S)-4- {[(2R)-4,9-dioxo-l ,2,8,9-tetrahydro-4H,7H-imidazo[ 1,2,3- ij]-l,8-naphthyridin-2-yl]methyl}-2-morpholinyl)methyl]carbamate In a 100 mL round-bottomed flask were (liS)-l-(hydroxymethyl)-l,2,5,6- tetrahydro-4H,9H-imidazo[l,2,3-ij]-l,8-naphthyridine-4,9-dione (for a preparation see Example 5A(g)) (450 mg, 2.043 mmol) and triethylamine (0.342 ml, 2.452 mmol) in DCM(20 ml) at 0 °C to give a orange solution. Methane sulfonylchloride (0.174 ml, 2.248 mmol) was added and the reaction was allowed to warm to rt and stirred for 1 h. LCMS indicated that the methanesulfonate had formed. The reaction mixture was diluted with DCM (100 mL) and washed with 2 X 25 mL of a saturated aqueous NaHCO3 solution. The organic phase was separated and dried over Na2SO4. The solution was concentrated under vacuum, and taken up in acetonitrile (20.00 ml). Pyridine (0.500 ml) was added followed by 1,1-dimethylethyl [(2R)-2-morpholinylmethyl]carbamate (for a synthesis see WO2008009700 Example 89(e)) (884 mg, 4.09 mmol), and the reaction was heated to 75 °C. The reation was stirred for 5h at which time LCMS indicated a complete reaction. The reaction was cooled to rt and concentrated under vacuum. The reaction mixture was diluted with DCM (100 mL) and washed with 25 mL of a saturated aqueous NaHCO3 solution. The organic phase was separated and dried over Na2SO4. The resulting residue was purified on silica 0-10% MeOH/DCM and the title compound (805 mg, 1.539 mmol, 75 % yield) was isolated as a red oil. MS(ES+)m/z419(MH+). (b)1,1-Dimethylethyl[((2S)-4-{[(1R)-4,9-dioxo-l,2-dihydro-4H,9H-imidazo[l,2,3-ij]- 1,8-naphthyridin-1-yl]methyl} -2-morpholinyl)methyl]carbamate To a 50 mL round-bottomed flask was added 1,1-dimethylethyl [((25)-4-{[(2R)- 4,9-dioxo-1,2,8,9-tetrahydro-4H,7H-imidazo[l,2,3-ij]-l,8-naphthyridin-2-yl]methyl}-2- morpholinyl)methyl]carbamate (805 mg, 1.924 mmol) in 1,4-dioxane (10 ml) at rt under -99- nitrogen to give a orange solution. DDQ (655 mg, 2.89 mmol) was added and the reaction became very dark. The reaction was heated to 90 °C on an oil bath and stirred for 30 min. The reaction was cooled to rt, 200 mL of a 5% aqueous K2CO3 solution was added and the reaction was extracted with DCM (3X200 mL). The combined organic layers were washed with saturated aqueous NaCl solution; the organic layer was separated and dried over Na2SO4, and concentrated to give the crude product. The crude product was added to a silica gel column and was eluted with 0 - 20% MeOH/CHCl3 to give the title compound (830 mg, 1.794 mmol, 93 % yield) as a red oil. MS(ES+)m/z417(MH+). (c) (1H)-1 - {[(2S)-2-(Aminomethyl)-4-morpholinyl]methyl} -1,2-dihydro-4H,9Himidazo[ l,2,3-ij]-l,8-naphthyridine-4,9-dione(HCl) To a 50 mL round-bottomed flask was added 1,1-dimethylethyl [((2S)-4-{[(lR)- 4,9-dioxo-1,2-dihydro-4H,9H-imidazo[ 1,2,3-ij]-1,8-naphthyridin-l -yl]methyl} -2- morpholinyl)methyl]carbamate (830 mg, 1.993 mmol) in DCM (10 ml) to give a brown solution. 4N HC1 in dioxane (2.491 ml, 9.96 mmol) was added and the reaction mixture stirred at rt. After 30 min the solution became cloudy so 2 mL of methanol was added and the reaction was stirred for another 30 min. The reaction was concentrated under vacuum to give the desired product as an HC1 salt (520 mg, 1.474 mmol, 74.0 % yield) as a brown solid which was used without further purification.. (d) Title Compound: To a 10 mL round-bottomed flask were added (1R)-l-{[(2S)-2-(aminomethyl)-4- morpholinyl]methyl} -1,2-dihydro-4H,9H-imidazo[ 1,2,3-ij]-l ,8-naphthyridine-4,9- dione(HCl) (85 mg, 0.241 mmol), [l,3]oxathiolo[5,4-c]pyridine-6-carbaldehyde (40.3 mg, 0.241 mmol) (for a synthesis see WO2004058144 Example 61), and NaHCO3 (60.7 mg, 0.723 mmol) in DCM (4 ml) and methanol (1 ml) to give a yellow suspension. The reaction was stirred overnight and sodium triacetoxyborohydride (102 mg, 0.482 mmol) was added. The reaction was stirred for 4h, then filtered through celite and the pad washed with 10% MeOH/DCM. Chromatography on silica eluting with 0-10% MeOH/CHCl3 (1% NH4OH) gave the free base of the title compound in which the LCMS, 1H NMR were consistent. 1H NMR δH D-4 MeOH, (400MHz) 2.02-2.13 (m, 1H), 2.34-2.49 (m, 2H), 2.60-2.78 (m, 2H), 2.85-3.08 (m, 2H), 3.40-3.68 (m, 2H), 3.72-3.89 (m, 1H), 4.40-4.51 (m, 2H), 4.89 (s, 2H), 5.10-5.20 (m, 1H), 5.80-5.89 (m, 2H), 6.23-6.38 (m, 2H), 7.72-7.82 (m, 2H), 7.90- 7.96 (m, 2H), MS (ES+) m/z 468 (MH+). The free base of the title compound was taken up in 10% MeOH/DCM and treated with 500 uL IN HC1 in ether. The solution was concentrated under vacuum to give the title compound as the diHCl salt (49 mg, 0.091 mmol, 37.6% yield) as a tan solid. -100- Example 48 (1R)-l-{[(25)-2-({[(7-Chloro-3-oxo-3,4-dihydro-2H-pyrido[3,2- b) [ 1,4] oxazin-6-yl)methyl] amino} methyl)-4-mo rpholinyl] methyl} -1,2-dihy dro- 4H,9H-imidazo[1,2,3-ij]-1,8-naphthyridine-4,9-dionedihydrochloride To a 10 mL round-bottomed flask were added (1R)-l-{[(2S)-2-(aminomethyl)-4- morpholinyl]methyl} -1,2-dihydro-4H,9H-imidazo[l ,2,3-ij]-l ,8-naphthyridine-4,9- dione(HCl) (for a preparation see Example 47(c)) (85 mg, 0.241 mmol), 7-chloro-3-oxo- 3,4-dihydro-2H-l,4-benzoxazine-6-carbaldehyde (51.0 mg, 0.241 mmol) (for a synthesis see WO2003064421, Example 15(c)), and NaHC03 (60.7 mg, 0.723 mmol) in DCM (4 ml) and methanol (1 ml) to give a yellow suspension. Na2S04 (171 mg, 1.205 mmol) was added, the reaction was stirred overnight, and sodium triacetoxyborohydride (102 mg, 0.482 mmol) was added. The reaction was stirred for 4h, filtered through celite, and the pad washed with 10% MeOH/DCM. Chromatography on silica eluting with 0 - 10% MeOH/CHCl3 (1% NH4OH) gave the free base of the title compound in which the LCMS, 1H NMR were consistent with desired product. 1H NMR δH D-4 MeOH, (400MHz) 2.05-2.13 (m, 1H), 2.32-2.49 (m, 2H), 2.59-2.80 (m, 2H), 2.88-3.07 (m, 3H), 3.42 (s, 2H), 3.40-3.49 (m, 1H), 3.58-3.67 (m, 1H), 3.72-3.96 (m, 3H), 4.42-4.51 (m, 2H), 4.68 (s, 2H), 5.10-5.18 (m, 1H), 6.22-6.35 (m, 2H), 7.39 (s, 1H) 7.72-7.80 (m, 2H). MS (ES+) m/z 513/515 (MH+). The free base of the title compound was taken up in 10% MeOH/DCM and treated with 500 uL 1N HC1 in ether. The solution was concentrated under vacuum to give the title compound as the diHCl salt (61 mg, 0.104 mmol, 43.2 % yield) as a pale yellow solid. Example 49 (2R)-2-({4-[([l,2,5]Thiadiazolo[3,4-6]pyridin-6-ylmethyl)amino]-lpiperidinyl} methyl)-1,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione -101- (a) 6-[(E)-2-Phenylethenyl][ 1,2,5]thiadiazolo[3,4-b]pyridme To 6-bromo[l,2,5]thiadiazolo[3,4-b]pyridine (for a preparation see Indian Journal of Chemistry, Section B: Organic Chemistry Including Medicinal Chemistry (1979), 17B(1), 13-16) (1.9 g, 8.79 mmol), [(E)-2-phenylethenyl]boronic acid (1.561 g, 10.55 mmol) and tetrakistriphenylphosphine palladium(0) (0.508 g, 0.440 mmol) was added 1,4-dioxane (38 ml) and then potassium carbonate (1.276 g, 9.23 mmol) in water (19 ml). The reaction was then stirred at reflux for 1.5 hours. The cooled reaction was partitioned between chloroform and water. The phases were separated with a hydrophobic frit and the organic extracts concentrated to give a black solid/gum (~2.4g). This crude material was purified by chromatography on silica eluting with 20-50% EtOAc in cyclohexane to give the product as a yellow/brown solid (0.88g). 1H NMR δH D6-DMSO 400MHz 7.36 (t, 1H), 7.45 (t, 2H), 7.55 (d, 1H), 7.70 (d, 2H), 7.78 (d, 1H), 8.3 (s, 1H), 9.51 (s, 1H) MS (ES+) m/z 240 (MH+). (b) [ 1,2,5]Thiadiazolo[3,4-6]pyridine-6-carbaldehyde To 6-[(E)-2-phenylethenyl][l,2,5]thiadiazolo[3,4-b]pyridine (0.88 g, 3.68 mmol) was added acetone (30 ml), N-methyl-morpholine-N-oxide, 50 wt.% in water (1.525 ml, 7.35 mmol) and then osmium tetroxide in water (0.225 ml, 0.037 mmol). The reaction was then stirred for 20 hours. To the pale brown solution was added sodium periodate (3.15 g, 14.71 mmol) and stirring continued for 45mins. The solvent was reduced by rotary evaporation and the remainder partitioned between chloroform and water. The aqueous was further extracted with chloroform and the combined organic extracts passed through a hydrophobic frit and concentrated to give brown/black solid (0.6g). A portion of this material (0.575g) was purified by chromatography on silica eluting with 20% EtOAc in cyclohexane to a pale yellow solid (160mg). 1H NMR δH D6-DMSO 400MHz 9.18 (s, 1H), 9.49 (s, 1H), 10.30 (s, 1H) (c) Title compound -102- To (2R)-2-[(4-aminocyclohexyl)methyl]-1,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylene- 3,8-dione dihydrochloride (for a preparation see Example 16A(j)) (100 mg, 0.333 mmol) was added chloroform (3 ml), methanol (0.300 ml) and triemylamine (0.139 ml, 0.999 mmol). The mixture was stirred for 20mins then [l,2,5]thiadiazolo[3,4-b]pyridine-6-carbaldehyde (52.2 mg, 0.316 mmol) was added. The reaction was stirred overnight then sodium triacetoxyborohydride (212 mg, 0.999 mmol) was added and stirring continued for 1 hour. Further sodium triacetoxyborohydride (212 mg, 0.999 mmol) was added and the reaction stirred for 1 hour. The reaction was partitioned between sat. NaHCCh and 20% MeOH in chloroform. The aqueous was further extracted with 20% MeOH in chloroform and the combined organic extracts were passed through a hydrophobic frit and concentrated. This crude material (~1 lOmg) was purified by chromtaography eluting with 0-20% MeOH in DCM to furnish product (46mg, 27%). This was freeze dried from 1,4-dioxane to give the title compound as a pale brown solid (45mg, 25%)). 1HNMR δH CDC13 400MHz 1.38 (m, 2H), 1.91 (t, 2H), 2.25 (dt, 1H), 2.35 (dt, 1H), 2.58 (m, 1H), 2.71 (m, 2H), 2.96 (br d, 1H), 3.16 (dd, 1H), 4.06 (s, 2H), 4.40 (dd, 1H), 4.56 (dd, 1H), 5.04 (m, 1H), 6.40 (d, 1H), 7.78 (d, 1H), 7.83 (s, 1H), 8.26 (s, 1H) MS(ES+)m/z451(MH+). Example 50 (1R)-1-({4-[(3,4-Dihydro-2H-chromen-7-ylmethyl)amino]-lpiperidinyl} methyl)-l,2-dihydro-4H,9H-imidazo[1,23-H]-l,8-naphthyridine-4,9- dione hydrochloride A solution of (1R)-1 -[(4-amino-1 -piperidinyl)methyl]-1,2-dihydro-4H,9Himidazo[ l,2,3-ij]-l,8-naphthyridine-4,9-dione dihydrochloride (for a preparation see example 5A(j)) (188 mg, 0.503 mmol) and triethylamine (0.175 ml, 1.256 mmol) in chloroform (4.5 ml) and methanol (0.5 ml) at it was stirred at rt for 15 min then 3,4- dihydro-2H-chromene-7-carbaldehyde (for a synthesis see WO2007067511 Example 19 (chromane-7-carbaldehyde)) (68 mg, 0.419 mmol) in chloroform (2 ml) was added dropwise at rt. The reaction mixture was stirred at rt for 1 h then sodium triacetoxyborohydride (444 mg, 2.096 mmol) was added in one portion and the reaction mixture was stirred at rt overnight. LCMS showed a mixture of product, some residual aldehyde. Additional sodium triacetoxyborohydride (267 mg, 1.258 mmol) was added and the reaction stirred at rt for 6 h. The reaction was quenched with NaHC03 (aq) (20 ml) and extracted with 20% MeOH/DCM (3 x 30 ml). The combined organic layers were dried over MgS04, filtered, evaporated and chromatographed (0-50% MeOH/DCM) to deliver the free base of the title compound as a pale yellow clear oil (49 mg, 0.11 mmol, -103- 1HNMR 8H CDCI3, (400MHz) 1.28-1.42 (m, 2H), 1.78-1.87 (m, 2H), 1.97-2.01 (m, 2H), 2.17-2.31 (m, 2H), 2.47-2.55 (m, 1H), 2.62-2.68 (m, 2H), 2.74-2.77 (m, 2H), 2.95 (d, 1H), 3.07 (dd, 1H) 3.71 (m, 2H), 4.17 (t, 2H), 4.35 (dd, 1H), 4.56 (dd, 1H), 4.96-5.02 (m, 1H), 6.23-6.31 (m, 2H), 6.72 (s, 1H), 6.77 (dd, 1H), 6.98 (d, 1H), 7.47-7.50 (m, 2H). MS (ES+) m/z 447 (MH+). The free base of the title compound in 2 ml DCM was treated with one equivalent of 1M HCl in diethyl ether and then evaporated to give the title compound as the mono- HC1 salt as a pale orange powder (51 mg, 25%). LCMS was consistent with product. Example 51 (1R)-l-({4-[(2,3-Dihydro-l-benzofuran-6-ylmethyl)amino]-lpiperidinyl} methyl)-l,2-dihydro-4H,9H-imidazo[l,2,3-ij]-l,8-naphthyridine-4,9- dione hydrochloride (a) 2,3-Dihydro-1 -benzofuran-6-carbaldehyde To a solution of 6-bromo-2,3-dihydro-l-benzofuran (190 mg, 0.955 mmol) in THF(4 ml) at -78 °C was added n-BuLi (1.313 ml, 2.100 mmol). The reaction mixture was stirred at -78 °C for 45 min then a solution of DMF (1.109 ml, 1.6 M in hexanes, 14.32 mmol) in THF (2 ml) was added dropwise and the reaction was stirred at -78 °C for 10 min then warmed to rt and stirred for 1 h. LCMS showed no starting material remaining. The reaction was stirred at rt for a further 2.5 h. The reaction mixture was poured cautiously into 2 M HCl (50 ml) and extracted with ethyl acetate (3 x 50 ml). The combined organic extracts were washed with brine (50 ml), dried over MgSO4, filtered, evaporated and chromatographed (eluting 0-100% EtOAc/Hexane). The relevant fractions were combined and evaporated to deliver the product as a clear, colourless oil (44 mg, 0.297 mmol, 31%). 1H NMR m CDCI3, (400MHz) 3.28 (t, 2H), 4.64 (t, 2H), 7.26 (s, 1H), 7.33-7.39 (m, 2H), 9.91 (s, 1H). (b) Title compound A solution of (1R)-1 -[(4-amino-1 -piperidinyl)methyl]-1,2-dihydro-4H,9Himidazo[ l,2,3-ij]-l,8-naphthyridine-4,9-dione dihydrochloride (for a preparation see Example 5A(j)) (133 mg, 0.356 mmol) and triethylamine (0.124 ml, 0.891 mmol) in chloroform (4.5 ml) and methanol (0.5 ml) at rt was stirred at rt for 15 min then 2,3- dihydro-l-benzofuran-6-carbaldehyde (44 mg, 0.297 mmol) in chloroform (2 ml) was added dropwise at rt. The reaction mixture was stirred at rt overnight. The reaction was quenched with NaHC03 (aq) (20 ml), extracted with 20% MeOH/DCM (3 x 30 ml). The cecabined organic layers were dried over MgSO4, filtered, evaporated and chromatographed (0-50% MeOH/DCM). The relevant fractions were combined and -104- evaporated to deliver the free base of the title compound as a pale yellow clear oil (18 mg, 0.04 mmol, 26%). 1H NMR δH CDC13, (400MHz) 1.40-1.53 (m, 2H), 1.85-1.92 (m, 2H), 2.16-2.30 (m, 2H), 2.54-2.68 (m, 3H), 2.98 (d, 1H), 3.08 (dd, 1H), 3.16 (t, 2H), 3.78 (s, 2H), 4.33-4.38 (m, 1H), 4.52-4.57 (m, 3H), 4.96-5.02 (m, 1H), 6.24 (d, 1H), 6.29 (d, 1H), 6.79 (s, 1H), 6.84 (d,lH), 7.13 (d, 1H), 7.47-7.50 (m, 2H). MS (ES+) m/z 433 (MH+). The free base of the title compound in 2 ml DCM was treated dropwise with 1 M HC1 in diethyl ether (0.04 ml, 0.04 mmol) to give the title compound as the mono-HCl salt as an orange powder (20 mg, 14%). Example 52 (1R)-l-({4-[(3,4-Dihydro-2H-chromen-6-ylmethyl)amino]-lpiperidinyl} methyl)-1,2-dihydro-4H,9H-imidazo[l,2,3-ij]-1,8-naphthyridine-4,9- dione hydrochloride O x y N ^ N ^ k N A solution of (1R)-l-[(4-amino-l-piperidinyl)methyl]-l,2-dihydro-4H,9Himidazo[ l,23-ij]-l,8-naphthyridine-4,9-dione dihydrochloride (for a preparation see Example 5A(j)) (12 mg, 0.032 mmol) and triethylamine (0.139 ml, 0.999 mmol) in DCM (4.5 ml) and methanol (0.5 ml) at rt was stirred for 5min. 3,4-Dihydro-2H-chromene-6- carbaldehyde (commericially available) (45 mg, 0.277 mmol) was added and the resulting solution was stirred overnight for 18h. LCMS showed that aldehyde remained and no amine remained. Additional (1R)-1-[(4-amino-l-piperidinyl)methyl]-l,2-dihydro-4H,9Himidazo[ l,2,3-ij]-l,8-naphthyridine-4,9-dione (120 mg, 0.321 mmol) and additional triethylamine (0.138 ml, 0.999 mmol) were added and the resulting mixture stirred for 1h. Additional sodium triacetoxyborohydride (294 mg, 1.387 mmol) was added and resulting solution was stirred for 60 h. The reaction was diluted with DCM (10ml) and sodium bicarbonate solution (10ml) and stirred at rt for 10mins and extracted with methanol:DCM (20%, 3x150ml). The combined organic extracts were dried (MgSO4), filtered, evaporated and chromatographed (0-50% methanol :DCM). The column waste was concentrated to afford a brown oil that was re-chromato graphed (0-50% methanol :DCM). The relevant fractions were combined to afford the free base of the title compound as a white solid (27 mg, 0.06 mmol, 22%). 1HNMR δH CDCl3, (400MHz) 1.76-1.86 (m, 2H), 1.91-1.97 (m, 2H), 2.04-2.06 (mm, 2H), 2.15 (t, 1H), 2.24 (t, 1H), 2.62 (dd, 1H), 2.75-2.81 (m, 4H), 3.06-3.14 (m, 2H), 3.85 (s, 2H), 4.12 (t, 2H), 4.40 (dd, 1H), 4.51 (dd, 1H), 4.96-5.02 (m, 1H), 6.22-6.29 (m, 2H), 6.77 (d, 1H), 7.25-7.27 (m, 2H), 7.47-7.50 (m, 2H). MS (ES+) m/z 447 (MH+). -105- The free base of the title compound in chloroform (5ml) and methanol (3ml) was treated dropwise with hydrochloric acid in ether (IM, 0.06ml, 0.06mmol) to give the title compound as the mono-HCl salt as a white solid (6 mg, 4%). Example 53 (2R)-2-[(4-{[(5-Fluoro-2,3-dihydro-1,4-benzodioxin-6- yl)methyl]amino}-1-piperidinyl)methyl]-l,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylene- 3,8-dione hydrochloride N (a) 5-Fluoro-2,3-dihydro-l ,4-benzodioxin A solution of 3-fluoro-l,2-benzenediol (5.278 g, 41.2 mmol) in DMF (50 ml) was treated with potassium carbonate (17.08 g, 124 mmol) and 1,2-dibromoethane (3.91 ml, 45.3 mmol) and stirred at rt for 72h. The reaction was treated with water (200ml) and extracted 3x200ml (EtOAc). The combined organic extracts were washed with water (200ml), brine (200ml), dried (MgSO4), evaporated and chromatographed (0-20% EtOAc-Cyclohexane) to give product as a clear oil. (2.437g, 38%). 1H NMR δH CDCl3, (400MHz) 4.22-4.39 (m, 4H), 6.60-6.82 (m, 3H). (b) 6-Bromo-5-fluoro-2,3-dihydro-l,4-benzodioxin solution of 5-fluoro-2,3-dihydro-l ,4- benzodioxin (0.335 g, 2.173 mmol) in methanol (10 ml) at 0 °C was treated with bromine (0.134 ml, 2.61 mmol) and allowed warm to rt over lOmin and stirred at rt for 18h. Reaction was then treated with saturated aqueous sodium metabisulfate (100ml), extracted 3 x 100ml (DCM), the combined organic extracts dried (MgSO/O, filtered, evaporated, chromatographed (0-50% EtOAC:Cyclohexane) to give product as a clear oil, which solidified in the freezer to give a white solid (35 lmg, 59%). lH NMR 8H CDC13, (400MHz) 4.20-4.39 (m, 4H), 6.52-6.65 (m, 1H), 6.91-7.05 (m, 1H). (c) 5-Fluoro-2,3-dihydro-l ,4-benzodioxin-6-carbaldehyde A solution of 6-bromo-5-fluoro-2,3-dihydro-l,4-benzodioxm (146 mg, 0.627 mmol) in THF(5 ml) at -78 °C was treated with n-BuLi (0.551 ml, 1.378 mmol) under a nitrogen atmosphere and stirred at -78 °C for 15 min before treatment with a solution of DMF (0.243 ml, 3.13 mmol) in THF)(2.00 ml). The reaction was stirred for 10 min at -78 °C and then the reaction was allowed warm to rt over 10 min and stirred at rt for 0.5h. Reaction was treated with 2M HCI (20ml) and extracted with ethyl acetate (3 x 100ml). The organic extracts were evaporated, dried (MgSO4), filtered, evaporated, chromatographed (0-100% EtOAC:Cyclohexane) to give product as a white solid (25mg, 22%). MS(ES+)m/zl83(MH+). -106- (d) Title compound A suspension of (2i?)-2-[(4-amino-l-piperidinyl)methyl]-l,2-dihydro-3H,8H- 2a.5,8a-triazaacenaphthylene-3,8-dione dihydrochloride (for a preparation see Example 16A(j), amine was converted into dihydrochloride after chiral hplc purification) (51.3 mg, 0.137 mmol) in chloroform (5 ml) and methanol (0.1 ml) at rt under argon was treated with triethylamine (0.057 ml, 0.411 mmol) and stirred at rt for 0.25h. The solution was then treated with 5-fluoro-2,3-dihydro-l,4-benzodioxin-6-carbaldehyde (24.95 mg, 0.137 mmol) and stirred for a further 0.5h. The solution was then treated with sodium triacetoxyborohydride (174 mg, 0.822 mmol) and stirred at rt for 2h, more sodium triacetoxyborohydride (174 mg, 0.137 mmol) was added, reaction stirred for a further lh, the reaction was then treated with saturated aqueous NaHCO3 (20ml) and extracted with 20% methanol/DCM (3 x 20ml). The combined organic extracts were dried (MgS04) and chromatographed (0-20% methanol:DCM) to give the free base of the title compound as a white solid (29mg, 0.062mmol, 45%,). 1H NMR δH CDC13, (400MHz) 1.20-1.46 (m, 2H),1.73-1.95 (m, 2H), 2.15-2.39 (m, 2H), 2.41-2.55 (m, 1H), 2.61-2.75 (m, 2H), 2.88-3.00 (m, 1H), 3.10-3.20 (m, 1H), 3.78 (s, 2H), 4.22-4.42 (m, 5H). 4.51-4.60 (m, 1H), 4.95-5.09 (m, 1H), 6.38 (d, 1H), 6.62 (m, 1H), 6.71-6.80 (m, 1H), 7.76 (d, 1H), 7.81 (s, 1H) MS (ES+) m/z 468 (MH+). The free base of the title compound (29mg) in DCM/MeOH 2:1 (5ml) was treated with 1M HC1 in diethyl ether 62ul) and then evaporated to give the title compound as the mono-HCl salt (31mg, 0.062mmol) as a yellow solid. Example 54 (1R)-l-{[(2S)-2-({[(7-fluoro-2,3-dihydro-l,4-benzodioxin-6- yl)methyl]amino}methyl)-4-morpholinyl]methyl}-1,2-dihydro-4H,9H-imidazo[1,2,3- ij]-1,8-naphthyridine-4,9-dione dihydrochloride To a 10 mL round-bottomed flask were added (l/?)-l-{[(25)-2-(aminomethyl)-4- morpholmyl]memyl}-l,2-dmydro-4H,9H-imidazo[l,2,3-ij]-l,8-naphthyridine-4,9- dione(HCl) (85 mg, 0.241 mmol) (for a preparation see Example 47(c)), 7-fluoro-2,3- dihydro 1,4-beazodioxin-6-carbaldehyde (48.3 mg, 0.265 mmol) (for a synthesis see WO2002056882, Example 23(a)), andNaHC03 (60.7 mg, 0.723 mmol) in DCM(4 ml) and methanol (1 ml) to give a yellow suspension. Sodium sulfate (171 mg, 1.205 mmol) -107- was added, the reaction was stirred overnight at which point sodium triacetoxyborohydride (102 mg, 0.482 mmol) was added. The reaction was stirred for 4h at which point LCMS showed the reaction to be complete. The reaction mixture was diluted with 10% MeOH in DCM (20 mL), filtered .adsorbed onto silica and purified by chromatography on silica eluting with 0 - 10 % MeOH/CHCl3 (1% NH4OH) to give the free base of the title compound in which the LCMS, ]H NMR were consistent with the desired product. 1H NMR δH D-4 MeOH, (400MHz) 2.03-2.10 (m, 1H), 2.33-2.49 (m, 2H), 2.51-2.68 (m, 2H), 2.83-2.95 (m, 2H), 2.99-3.07 (m, 1H), 3.39 (s, 2H), 3.41-3.50 (m, 1H), 3.55-3.63 (m, 1H), 3.68-3.80 (m, 3H), 4.42-4.51 (m, 2H), 4.79 (s, 2H), 5.08-5.18 (m, 1H), 6.22-6.32 (m, 2H), 6.58-6.62, (m, 1H), 6.81-6.88 (m, 1H), 7.73-7.80 (m, 2H). MS (ES+) m/z 483 (MH+). The free base of the title compound was diluted in 5 % MeOH/CHCl3 and treated with IN HC1 in ether 100 uL and concentrated to give the title compound as the diHCl salt (55 mg, 0.099 mmol, 41.1 % yield) as a pale yellow solid. Example 55 (1R)-1-[((35)-3-{[([1,3]Oxathiolo[5,4-c]pyridin-6- ylmethyI)aminolmethyl}-l-pyrrolidinyl)methyl]-l,2-dihydro-4H,9H-iniidazo[1,2,3- ij]-1,8-naphthyridine-4,9-dionedihydrochloride -S > o (a) 1,1 -Dimethylethyl (35)-3- {[(trifluoroacetyl)amino]methyl} -1 - pyrrolidinecarboxylate To a 100 mL round-bottomed flask was added 1,1-dimethylethyl (3S)-3- (aminomethyl)-l-pyrrolidinecarboxylate (commercially available) (750 mg, 3.74 mmol) in (DCM) (20 ml) to give a colorless solution. Triethylamine (1.044 ml, 7.49 mmol) was added and the reaction was cooled to 0 °C. Trifluoroaceticanhydride (0.635 ml, 4.49 mmol) was added and the reaction was allowed to warm to rt while stirring for 14h. The solution was diluted with 100 mL DCM and washed with saturated aqueous solution of NaHCOs, and a saturated aqueous solution of NaCl. The organic layer was separated, dried over Na2S04, filtered and concentrated. The residue was subjected to chromatography on silica to give the product (0.990 g, 3.34 mmol, 89 % yield) as a pale yellow oil. MS (ES+) m/z 297 (MH+). (b) 2,2,2-Trifluoro-N-[(3R)-3-pyrrolidinylmethyl]acetamide hydrochloride -108- To a 100 mL round-bottomed flask was added 1,1-dimethylethyl (3S)-3- {[(trifluoroacetyl)amino]methyl}-l-pyrrolidinecarboxylate (830 mg, 2.80 mmol) in DCM (25 ml) at 25 °C to give a colorless solution. 4N HC1 (3.50 ml, 14.01 mmol) in dioxane was added and the reaction was allowed to stir o/n. The reaction was concentrated under vacuum to give the desired compound as colorless oil which was used in the next reaction without further purification. Isolated 2,2,2-trifluoro-N-[(3R)-3- pyrrolidinylmethyljacetamide (550 mg, 2.364 mmol, 84 % yield). MS(ES+)m/zl97(MH+). (c) N-[((3S)-1 - {[(2R)-4,9-dioxo-1,2,8,9-tetrahydro-4H,7H-imidazo[ 1,2,3-ij]-1,8- naphthyridin-2-yl]methyl}-3-pyrrolidinyl)methyl]-2,2,2-trifluoroacetamide To a 100 mL round-bottomed flask was added (liS)-l-(hydroxymethyl)-l,2,5,6- tetrahydro-4H,9H-imidazo[l,2,3-ij]-l,8-naphthyridine-4,9-dione HC1 (for a preparation see Example 47(c)) (350 mg, 1.589 mmol), triethylamine (0.266 ml, 1.907 mmol) in DCM (20 ml) 0 °C to give a orange solution. Methane sulfonylchloride (0.135 ml, 1.748 mmol) was added and the reaction was allowed to warm to rt and stir for 1 h. LCMS indicated that the methanesulfonate had formed. The reaction was diluted with DCM (100 mL) and washed with 2X25 mL of a saturated aqueous NaHCO3 solution. The organic phase was separated and dried over Na2SO4. The solution was concentrated under vacuum, diluted with acetonitrile (20.00 ml) and pyridine (0.500 ml) was added. 2,2,2- trifluoro-N-[(3R)-3-pyrrolidinylmethyl]acetamide (550 mg, 2.364 mmol) was added and the reaction was heated to 80 °C and stirred for 25h. LCMS indicated a complete reaction. The reaction was cooled to rt and concentrated under vacuum. The reaction mixture was diluted with DCM (100 mL) and washed with 25 mL of a saturated NaHCCb solution. The organic phase was separated and dried over Na2SO4. The crude product was purified on silica eluting with 0-15% MeOH/DCM to give product (240 mg, 0.602 mmol, 37.9 % yield) as a pale yellow oil. MS (ES+) m/z 399 (MH+). (d)N-[((31S)-l-{[(1R)-4,9-dioxo-l,2-dihydro-4H,9H-imidazo[l,2,3-ij]-l,8-naphthyridin- 1 -yl]methyl} -3-pyrrolidinyl)methyl]-2,2,2-trifluoroacetamide To a 25 mL round-bottomed flask was added N-[((3S)-1-{[(2R)-4,9-dioxo- 1,2,8,9-tetrahydro-4H,7H-imidazo[ 1,2,3-ij]-1,8-naphthyridin-2-yl]methyl} -3- pyrrolidinyl)methyl]-2,2,2-trifluoroacetamide (240 mg, 0.602 mmol) in 1,4-dioxane (5 ml) at rt under nitrogen to give a orange solution. DDQ (205 mg, 0.904 mmol) was added and the reaction became very dark. The reaction was heated to 80 °C on an oil bath and stirred for 10 h. The reaction was cooled to rt. 5% Aqueous K2CO3 (20 mL) was added and the reaction was extracted with DCM (3X100 mL). The combined organic layers were washed with a saturated aqueous NaCl solution and the organic phase was dried over Na2S04, filtered and concentrated to give the crude product. The crude product was added to a srhca gei eotemn and was eluted with 0 - 20% MeOH/CHCl3 to give product (85 mg, 0.214 mmol, 35.6 % yield) as an orange solid. MS (ES+) m/z 397 (MH+). -109- (e)(1R)-l-{[(3S)-3-(aminomethyl)-l-pyrrolidinyl]methyl}-l,2-dihydro-4H,9Himidazo[ 1,2,3-ij]-1,8-naphthyridine-4,9-dione To a 25 mL round-bottomed flask was added N-[((35)-l-{[(1R)-4,9-dioxo-l,2- dihydro-4H,9H-imida2o[l,2,3-ij]-l,8-naphthyridin-l-yl]methyl}-3-pyrrolidinyl)methyl]- 2,2,2-trifluoroacetamide (85 mg, 0.214 mmol) in methanol (9 ml) and water (1.00 ml) to give a yellow solution. Potassium carbonate (59.3 mg, 0.429 mmol) was added and the reaction was stirred overnight. LCMS indicated a complete reaction. The reaction was diluted with 20% MeOH/DCM (100 mL), dried over Na2S04, filtered and concentrated to give the product (60 mg, 0.200 mmol, 93 % yield) as an orange solid. MS(ES+)m/z301(MH+). (f) Title compound To a 10 mL round-bottomed flask were added (1R)-l-{[(3S)-3-(aminomethyl)-lpyrrolidinyl] methyl}-l,2-dihydro-4H,9H-imidazo[l,2,3-ij]-l,8-naphthyridine-4,9-dione (60 mg, 0.200 mmol), [l,3]oxathiolo[5,4-c]pyridine-6-carbaldehyde (33.4 mg, 0.200 mmol) (for a synthesis see WO2004058144 Example 61), and sodium sulfate (250 mg, 1.760 mmol) in DCM(4 ml) and methanol (1.00 ml) to give an orange suspension. The reaction was stirred overnight under nitrogen. Sodium triacetoxyborohydride (85 mg, 0.400 mmol) was added and the reaction was stirred for 3h. The reaction mixture was diluted with 10% MeOH/DCM (20 mL), filtered, adsorbed onto silica and then purified by silica chromatography ehiting with 0-10% MeOH/DCM (1% NH4OH) to give the free base of the title compound as a yellow oil. LCMS/NMR consistent with the desired product. 1H NMR δH D-4 MeOH, (400MHz) 1.34-1.43 (m, 1H), 1.85-1.96 (m, 1H), 2.15-2.30 (m, 2H), 2.38-2.51 (m, 3H), 2.68-2.75 (m, 1H), 2.04-3.17 (m, 2H), 3.41-3.50 (m, 1H), 3.69 (s, 2H), 4.42-4.51 (m, 2H), 5.08-5.18 (m, 1H), 5.83 (s, 2H), 6.26-6.32 (m, 2H), 7.33 (s, 1H), 7.73-7.78 (m, 2H), 7.94 (s, 1H). MS (ES+) m/z 452 (MH+). The free base of the title compound was diluted with 10% MeOH/CHCl3, 100 uL of IN HC1 in ether was added and the mixture concentrated under vacuum to give the title compound as the dihydrochloride salt (47 mg, 0.090 mmol, 44.9 % yield) as a tan solid. Example 56 7-{[(l-{[(1R)-3,8-Dioxo-l,2-dihydro-3H,8H-2a,5,8atriazaacenaphthylen- l-yl]methyl}-4-piperidinyl)amino]methyl}-2,3-dihydro-l,4- benzodioxin-5-carbonirrile hydrochloride -110- A suspension of (lJ?)-l-[(4-amino-l-piperidinyl)methyl]-l,2-dihydro-3H,8H- 2a,5,8a-triazaacenaphthylene-3,8-dione dihydrochloride (for a preparation see Example 13(k) or 15(d)) (50mg, 0.100 mmol) in chloroform (4 ml) and methanol (0.121 ml) at rt under nitrogen was treated with triethylamine (0.587 ml, 4.21 mmol) and stirred for 15min (the suspension turned into a solution). 7-Formyl-2,3-dihydro-l,4-benzodioxin-5- carbonitrile (for a synthesis see WO06014580 Preparation 13 or WO2007122258, Example 31(d)) ((18.95 mg, 0.100 mmol) was then added and the reaction was stirred for 30min. Sodium triacetoxyborohydride (63.7 mg, 0.301 mmol) was then added and the reaction was stirred for lh. LC-MS after lh showed some imine intermediate so more sodium triacetoxyborohydride (63.7 mg, 0.301 mmol) was added and the reaction stirred for 2h. LCMS after this time still showed imine intermediate, More sodium triacetoxyborohydride (63.7 mg, 0.301 mmol) was added and the reaction left stirring overnight (16h), LCMS after this time showed no starting material. Saturated NaHC03 (lOmL) was added followed by 20% MeOH/DCM (20ml) and the aqueous phase was extracted and then separated from the organic layer. The aqueous phase was extracted again with 20%MeOH/DCM (2x20ml). The combined organic extracts were dried (NaSO4), filtered and evaporated to give crude product. The crude product was purified on a silica column (0-20%MeOH/DCM) to give the free base of the title compound (33mg, 69.4%). 1HNMR δH CDC13, (400MHz) 1.15-1.41 (m, 2H), 1.72-1.91 (m, 2H), 2.19-2.39 (m, 2H), 2.40-2.52 (m, 1H), 2.53-2.78 (m, 2H), 2.89-2.98 (m, 1H), 3.02-3.14 (m, 1H), 3.68 (s, 2H), 4.22-4.49 (m, 5H), 4.51-4.62 (m, 1H), 4.98-5.08 (m, 1H), 6.32 (d, 1H), 7.06 (m, 2H), 7.78 (d, 1H), 7.88 (s, 1H). MS (ES+) m/z 475 (MH+). The free base of the title compound was dissolved in a small amount of DCM and treated with one equivalent of 1M HC1 in diethyl ether. This gave the title compound as the mono HC1 salt (33mg, 65%). Example 57 (1R)-1-[(4-{[(7-Fluoro-2,3-dihydro-l,4-benzodioxin-6- yl)methylJamino}-l-piperidinyI)methyI]-l,2-dihydro-3H,8H-2a,5,8a- - 1 1 1 - triazaacenaphthylene-3,8-dione hydrochloride A suspension of (1R)-l-[(4-amino-l-piperidinyl)methyl]-l,2-dihydro-3H,8H- 2a,5,8a-triazaacenaphthylene-3,8-dione dihydrochloride (for a preparation see Example 13(k)) or 15(d) (50 mg, 0.100 mmol) in chloroform (3 ml) and methanol (0.150 ml) at room temperature under nitrogen was treated with triethylamine (0.042 ml, 0.301 mmol) and stirred for 0.25h (the suspension turned into a solution). 7-Fluoro-2,3-dihydro-l,4- benzodioxin-6-carbaldehyde (for a synthesis see WO2002056882, Example 23(a)) (18.25 mg, 0.100 mmol) was then added and the reaction was stirred at room temperature for 0.5h. Sodium triacetoxyborohydride (67.1 mg, 0.301 mmol) was then added and the reaction was stirred at room temperature. After lh more sodium triacetoxyborohydride (67.1 mg, 0.301 mmol) was added and the reaction stirred at rt overnight. More sodium triacetoxyborohydride (67.1 mg, 0.301 mmol) was then added and the reaction stirred at rt. After lh no starting material remained. Saturated NaHCO3 (30mL) was added followed by 20% MeOH/DCM (30mL) and the aqueous phase was extracted and then separated from the organic layer. The aqueous phase was extracted again twice with 20%MeOH/DCM (2x30mL). The combined organic extracts were dried NaSO4, filtered and evaporated to afford the crude product. The crude product was purified by chromatography on silica (0-20%MeOH/DCM) to afford 35mg of the free base of the title compound as a yellow solid (74.7%). 1HNMR δH CDC13, (400MHz) 1.21-1.41 (m, 2H), 1.76-1.92 (m, 2H), 2.10-2.39 (m, 2H), 2.41-2.52 (m, 1H), 2.61-2.79 (m, 2H), 2.86-2.98 (m, 1H), 3.05-3.14 (m, 1H), 3.72 (s, 2H), 4.18-4.29 (m, 4H), 4.38-4.43 (m, 1H), 4.51-4.62 (m, 1H), 4.94-5.05 (m, 1H), 6.33 (d, 1H), 6.58 (d, 1H), 6.80 (1H, d), 7.77 (d, 1H), 7.86 (s, 1H). MS(ES+)m/z468(MH+). The free base of the title compound was dissolved in a small amount of DCM/MeOH and treated with leq of a 1M solution of HC1 in Et20. The solvents were removed and the solid dried in the desiccator (P2O5) to afford the title compound as the hydrochloride salt as a dark yellow solid (36mg). Example 58 (1R)-l-[(4-{[(8-Fluoro-2,3-dihydro-l,4-benzodioxin-6- yl)methyl]aminol-1-piperidinyl)methyl]-1,2-dihydro-3H,8H-2a,5,8a- -112- triazaacenaphthylene-3,8-dione hydrochloride N A suspension of (1R)-l-[(4-amino-l-piperidinyl)methyl]-l,2-dihydro-3H,8H- 2a,5,8a-triazaacenaphthylene-3,8-dione dihydrochloride (for a preparation see Example 13(k) or 15(d)) (50 mg, 0.100 mmol) in chloroform (25 ml) and methanol (1.250 ml) at room temperature under nitrogen was treated with triethylamine (0.042 ml, 0.301 mmol) and stirred for 0.25h (the suspension turned into a solution). 8-Fluoro-2,3-dihydro-l,4- benzodioxin-6-carbaldehyde (for a synthesis see WO2007122258, Example 8(b))(19.62 mg, 0.100 mmol) was then added and the reaction was stirred at room temperature for 0.5h. Sodium triacetoxyborohydride (67.1 mg, 0.301 mmol) was then added and the reaction was stirred at room temperature. After lh still starting material so sodium triacetoxyborohydride (67.1 mg, 0.301 mmol) was added and the reaction stirred at rt overnight. Still starting material so sodium triacetoxyborohydride (67.1 mg, 0.301 mmol) was added and the reaction stirred at rt for lh. Saturated NaHCO3 (30mL) was added followed by 20% MeOH/DCM (30mL) and the aqueous phase was extracted and then separated from the organic layer. The aqueous phase was extracted again twice with 20%MeOH/DCM (2x30mL). The combined organic extracts were dried NaSO4, filtered and evaporated to afford the crude product. The crude product was purified by chromatography on silica (0-20%MeOH/DCM) to afford 26mg of the free base of the title compound as a yellow solid. 1HNMR δH CDC13, (400MHz) 1.20-1.41 (m, 2H), 1.72-1.89 (m, 2H), 2.09-2.35 (m, 2H), 2.42-2.52 (m, 1H), 2.55-2.78 (m, 2H), 2.85-2.99 (m, 1H), 3.08-3.15 (m, 1H), 3.63 (s, 2H), 4.22-4.48 (m, 5H), 4.51-4.63 (m, 1H), 4.95-5.06 (m, 1H), 6.32 (d, 1H), 6.61-6.72 (m, 2H), 7.75 (d, 1H), 7.89 (s, 1H). MS (ES+) m/z 468 (MH+). The free base of the title compound was dissolved in a small amount of DCM/MeOH and treated with leq of a 1M solution of HC1 in Et20. The solvents were removed and the solid dried in the desiccator (P2O5) overnight to afford the title compound as the hydrochloride salt as a yellow solid (26.6mg, consistent with product). Example 59 (1R)-l-[(4-{[(2-Oxo-2H-chromen-7-yl)methyl]amino}-lpiperidinyl) methyl]-l,2-dihydro-4H,9H-imidazo[1,2,3-ij]-l,8-naphthyridine-4,9- dione -113- To a 10 mL round-bottomed flask were added (lR)-l-[(4-amino-lpiperidinyl) methyl]-l ,2-dihydro-4H,9H-imidazo[ 1,2,3-ij]-l ,8-naphthyridine-4,9-dione dihydrochloride (for a preparation see Example 5A(j)) (80 mg, 0.266 mmol), 2-oxo-2Hchromene- 7-carbaldehyde (for a synthesis see WO2008009700 Example 224) (46.4 mg, 0.266 mmol), andNaHC03 (100 mg, 1.190 mmol) in dichloromethane (DCM) (4 ml) and methanol (1 ml) to give a brown solution. Sodium sulfate (200 mg, 1.408 mmol) was added and the reaction was allowed to stir at rt overnight. After 15h sodium triacetoxyborohydride (113 mg, 0.533 mmol) was added and the reaction was allowed to stir at 25 °C under nitrogen for 4h. The reaction mixture was adsorbed onto silica and purified using 0 -10% MeOH/DCM (1% NH4OH) to give the title compound as a free base (30 mg, 0.064 mmol, 24.07 % yield) as a tan solid.. LCMS & 1H NMR consistant with desired product. 1H NMR δH D-4 MeOH, (400MHz) 1.20-1.39 (m, 2H), 1.72-1.89 (m, 2H), 1.90-2.09 (m, 1H), 2.13-2.31 (m, 2H), 2.39-2.50 (m, 1H), 2.56-2.70 (m, 2H), 2.90-3.10 (m, 2H), 3.83 (s, 2H), 4.32-4.58 (m, 2H), 4.98-5.18 (m, 1H), 6.20-6.39 (m, 3H), 7.19-7.28 (m, 2H), 7.39- 7.51 (m, 3H), 7.62-7.71 (m, 1H). MS(ES+)m/z 459(MH+). Example 60 (1R)-l-[(4-{[(2-oxo-2H-chromen-7-yl)methyl]amino}-lpiperidinyl) methyl]-l,2-dihydro-4H,9H-imidazo[l,23-ij]-l,8-naphthyridine-4,9- dione To a 10 mL round-bottomed flask were added (1R)-l-[(4-amino-lpiperidinyl) methyl]-1,2-dihydro-4H,9H-imidazo[ 1,2,3-ij ]-1,8-naphthyridine-4,9-dione dihydrochloride (for a preparation see Example 5A(j)) (45 mg, 0.134 mmol), 7-chloro- 3,4-dihydro-2H-pyrido[3,2-b][l,4]oxazine-6-carbaldehyde (prepared by (1) reduction of 7-chloro-3-oxo-3,4-dihydro-2H-pyrido[3,2-b][1,41oxazine-6-carbaldehyde(fora synthesis see WO2003064421 Example 15(c)) with LiAlH4 to give (7-chloro-3,4- dihydro-2H-pyrido[3,2-b][l,4]oxazin-6-yl)methanol and then (2) oxidation with MnO2) -114- (29.2 mg, 0.147 mmol), andNaHC03 (100 mg, 1.190 mmol) in dichloromethane (DCM) (4 ml) and methanol (1 ml) to give a brown suspension. Sodium sulfate (200 mg, 1.408 mmol) was added and the reaction was stirred at rt overnight. After 15h sodium triacetoxyborohydride (56.6 mg, 0.267 mmol) was added and the reaction was stirred at 25 °C under nitrogen for 4h. The reaction mixture was adsorbed onto silica and purified using 0-10% MeOH/DCM (1% NH4OH) to give the title compound as a free base (9.4 mg, 0.019 mmol, 14.57 % yield). 1H NMR δH D-4 MeOH, (400MHz) 1.49-1.70 (m, 2H), 2.00-2.17 (m, 2H), 2.28-2.49 (m, 2H), 2.72-2.81 (m, 1H), 2.89-2.94 (m, 1H), 3.05-3.20 (m, 3H), 3.38 (s, 2H), 3.50-3.58 (m, 1H), 4.18 (s, 2H) 4.20-4.24 (m, 2H), 4.43-4.50 (m, 2H), 5.08-5.18 (m, 1H), 6.28-6.35 (m, 2H), 7.06 (s, 1H), 7.78-7.83 (m, 2H). MS (ES+) m/z 483/485 (MH+). Example 61 (1R)-l-[(4-{[(2-oxo-2H-chromen-7-yl)inethyl]amino}-lpiperidinyl) methyl]-l,2-dihydro-4H,9H-imidazo[l,2,3-ij]-l,8-naphthyridine-4,9- dione To a 10 mL round-bottomed flask were added (1R)-l-[(4-amino-lpiperidinyl) methyl] -1,2-dihydro-4H,9H-imidazo[ 1,2,3 -ij ] -1,8-naphthyridine-4,9-dione dihydrochloride (for a preparation see Example 5A(j)) (75 mg, 0.223 mmol), 7-chloro- 3,4-dihydro-2H-pyrido[3,2-b][l,4]thiazine-6-carbaldehyde (prepared by (1) reduction of 7-chloro-3-oxo-3,4-dihydro-2H-pyrido[3,2-b][l ,4]thiazine-6-carbaldehyde (for a synthesis see WO2003087098 Example 306(e)) with LiAlH4, to give (7-chloro-3,4- dihydro-2i/-pyrido[3,2-6][l,4]thiazin-6-yl)methanol and then (2) oxidation with MnCh)) (47.8 mg, 0.223 mmol), andNaHC03 (100 mg, 1.190 mmol) in DCM (4 ml) and methanol (1 ml) to give a brown suspension. Sodium sulfate (200 mg, 1.408 mmol) was added and the reaction was allowed to stir at rt overnight. After 15h sodium triacetoxyborohydride (94 mg, 0.445 mmol) was added and the reaction was allowed to stir at 25 °C under nitrogen for 4h. The reaction mixture was adsorbed onto silica and purified using 0 -10% MeOH/DCM (1% NH40H) to give the title compound as a free base (45 mg, 0.090 mmol, 40.5 % yield). LCMS & 1H NMR were consistent with the desired product. 1HNMR δH D-4 MeOH, (400MHz) 1.20-1.45 (m, 2H), 1.65-1.92 (m, 2H), 2.19-2.39 (m, 2H), 2.42-2.71 (m, 2H), 2.82-3.10 (m, 5H), 3.65-3.80 (m, 4H), 4.40-4.50 (m, 2H), 5.05- 5.20 (m, 1H), 6.25-6.35 (m, 2H), 7.22 (s, 1H), 7.72-7.83 (m, 2H). MS (ES+) m/z 483/485 (MH+). -115- Example 62 (1R)-l-({4-[(3,4-dihydro-2H-pyrido[3,2-61[l,4]thiazin-6- ylmethyl)amino]-l-piperidinyl}methyl)-1,2-dihydro-4H,9H-imidazo[l,2,3-ij]-l,8- naphthyridine-4,9-dione VVV0 A suspension of (1R)-1 -[(4-amino-1 -piperidinyl)methyl]-1,2-dihydro-4H,9Himidazo[ l,2,3-ij]-l,8-naphthyridine-4,9-dione dihydrochloride (for a preparation see Example 5A(j)) (0.075g, 0.201 mmol) in dichloromethane (5 ml) and methanol (1 ml) at rt under argon was treated with 3,4-dihydro-2H-pyrido[3,2-b][l,4]thiazine-6- carbaldehyde (prepared by (1) reduction of 3-oxo-3,4-dihydro-2H-pyrido[3,2- b][l,4]thiazine-6-carboxaldehyde (for a synthesis see WO2003087098, Example 301(d)) with LiAlFLi to give 3,4-dihydro-2H-pyrido[3,2-6][l,4]thiazin-6-ylmethanol and then (2) oxidation with Mn02)) (0.036 g, 0.201 mmol), sodium bicarbonate (0.150 g, 1.786 mmol) and sodium sulfate (0.300 g, 2.112 mmol) and stirred at rt for 5h. The solution was then treated with sodium triacetoxyborohydride (0.128 g, 0.603 mmol) and stirred over weekend for 65 hours. The solution was evaporated, taken up in CH3OH, adsorbed onto silica gel and chromatographed on silica (0-15% CH3OH in DCM(with 1% NH4OH)) to give the title compound as the free base (59mg, 63%) as a beige solid. 1HNMR DMSO-D6, (400MHz) 1.02-1.27 (m, 2H),1.61-1.78 (m, 2H), 1.81-1.96 (s, 1H), 2.01-2.19 (m, 2H), 2.28-2.38 (m, 1H), 2.67-2.79 (m, 1H), 2.82-2.98 (m, 4H), 3.50 (s, 2H), 3.55-3.61(m, 2H), 4.21-4.34 (m, 2H), 4.91-4.99 (m, 1H), 6.10-6.19 (m, 2H), 6.48-6.52 (d, 1H), 6.69-6.75 (s, 1H), 7.12-7.19 (d, 1H), 7.71-7.82 (d, 2H) MS (ES+) m/z 465 (MH+). Example 63 l-[(4-{[(3-Oxo-3,4-dihydro-2H-1,4-benzothiazin-6-yl)methyl]amino}- l-piperidinyl)methyl]-l,2-dihydro-4H,9H-imidazo[l,2,3-ij]-l,8-naphthyridine-4,9- dione hydrochloride (2:1 mixture of R:S) o- The title compound was prepared from l-[(4-amino-l-piperidinyl)methyl]-l,2- dihydro-4H,9H-imidazo[l,2,3-ij]-1,8-naphthyridine-4,9-dione (2:1 mixture ofR.S, for a preparation see Example 11(d)) and 2,3-dihydro[l,4]oxathiino[2,3-c]pyridine-7- carbaldehyde (for a synthesis see WO2004058144, 60) according to the general method of Example 12. 1H NMR, LC-MS and mono-hydrochloride salt formation as for Example 6A. -116- Example 64 l-({4-[([lr3]oxathiolo[5,4-c]pyridin-6-yImethyl)amino]-lpiperidinyljmethyl)- 1,2-dihydro-4H,9H-imidazo[1,2,3-ij]-1,8-naphthyridine-4,9- dione (2:1 mixture of S:R) The title compound was prepared from l-[(4-amino-l-piperidinyl)methyl]-l,2- dihydro-4H,9H-imidazo[l,2,3-ij']-l,8-naphthyridine-4,9-dione (2:1 mixture of S:R prepared analogously to Example 1 l(a -d)) but using 7-(methyloxy)-l-[(2R)-2- oxiranylmethyl]-3,4-dihydro-l,8-naphthyridin-2(lH)-one (for a synthesis see example 5(f)) and 2,3-dihydro[l,4]oxathiino[2,3-c]pyridine-7-carbaldehyde (for a synthesis see WO2004058144, Example 60) according to the general method of Example 12. 1H NMR, LC-MS and mono-hydrochloride salt formation as for Example 6A. Table 1: Made using the specified starting materials according to the method of Example 5(k) Example number 65 66 Salt form di-HCl MS (ES+) m/z 432(MH+) Free base MS (ES+) m/z431(MH+) Structure XXX H °XXJ° Starting materials (for a preparation see referenced examples) (1R)-l-[(4-Amino-lpiperidinyl) methyl]-l,', dihydro-4H,9Himidazo[ l ,2,3-y]-1,8- naphthyridine-4,9-dioii dihydrochloride (Example 5A(j)) lH-imidazo[4,5- b]pyridine-2- carbaldehyde (commercial) (l/?)-l-[(4-Amino-lpiperidinyl) methyl]-1 ^ dihydro-4H,9Himidazo[ 1,2,3-z/]-1,8- naphthyridine-4,9-dion dihydrochloride (Example 5AQ)) -117- 67 68 di-HCl MS(ES+) m/z 480(MH+) di-HCl MS (ES+) m/z 466(MH+) xxx H XX7 lH-pyrrolo[2,3- b]pyridine-2- carbaldehyde (commercial) (1R)-l-[(4-Amino-lpiperidinyl) methyl] -1,2- dihydro-4H,9Himidazo[ l ,2,3-y]-1,8- naphthyridine-4,9-dione dihydrochloride (Example 5A(j)) 8-Fluoro-3 -oxo-3,4- dihydro-2/7-1,4- benzoxazine-6- carbaldehyde (for a synthesis see WO2006014580 Preparation 15) (1R>l-[(4-Amino-lpiperidinyi) methyl]-l ,2- dihydro-4i/,9Himidazo[ l ,2,3-ij]-1,8- naphthyridine-4,9-dione dihydrochloride (Example 5A(j)) 8-fluoro-3,4-dihydro-2H- 1,4-benzoxazine-6- carbaldehyde Prepared by (1) reduction of 8-fluoro-3-oxo-3,4- dihydro-2H-l,4- benzoxazine-6- carbaldehyde (for a synthesis see WO2006014580 Preparation 15) with LiAlHU to give (8- fruoro-3,4-dihydro-2H- 1,4-benzoxazin-6- yl)methanol and then (2) -118- 69 10 71 Free base MS (ES+) m/z 484(MH+) Free base MS (ES+) m/z 513/515(MH+) Mono-HCl MS (ES+) m/z 453(MH+) H / A )~->r\\ V / XU XU oxidation with Mn02)) (1R)-l-[(4-Amino-lpiperidinyl) methyl]-l ,2- dihydro-4H,9tfimidazo[ 1,2,3-zj"]-1,8- naphthyridine-4,9-dione dihydrochloride (Example 5A(j)) 7,8-Difluoro-3,4- dihydro-2H-l,4- benzoxazine-6- carbaldehyde Prepared by(l) reduction of methyl 7,8- difluoro-3 -oxo-3,4- dihydro-2i/-l,4- benzoxazine-6- carboxylate with LLA1H4 to give (7,8-difluoro-3,4- dihydro-2H-l,4- benzoxazin-6- yr)methanoI and then (2) oxidation with Mn02)) (l/?)-l-[(4-Amino-lpiperidinyl) methyl]-l ,2- dihydro-4H,9Himidazo[ l ,2,3-y]-1,8- naphthyridine-4,9-dione dihydrochloride (Example 5A(j)) 7-Chloro-3 -oxo-3,4- dihydro-2H-pyrido[3,2- b][l,4]thiazine-6- carbaldehyde (for a synthesis see WO03087098 Ex306(e)) (1R>l-[(4-Amino-lpiperidinyl) methyl]-1,2- dihydro-4H,9Himidazo[ l ,2,3-y]-l ,8- naphthyridine-4,9-dione -119- 72 73 74 Mono-HCl MS (ES+) m/z 454(MH+) Mono-HCl MS (ES+) m/z 454(MH+) Di-HCl MS (ES+) m/z 448(MH+) ,-rOtrOQ O ^ N - s ^ N ^ O O—' VXNJ dihydrochloride (Example 5A(j)) 6,7-Dihydro-5Hthieno[ 3,2-6]pyran-2- carbaldehyde (for a synthesis see WO2007122258 Example 88(c)) (2H)-2-[(4-Amino-lpiperidinyl) methyl]-l ,2- dihydro-3H,8H-2a,5,8atriazaacenaphthylene- 3,8-dione (Example 16A(j) method B) 6,7-Dihydro-5Hthieno[ 3,2-6]pyran-2- carbaldehyde (for a synthesis see WO2007122258 Example 88(c)) (lR)-l-[(4-Amino-lpiperidinyl) methyl]-1,2- dihydro-3H,8H-2a,5,8atriazaacenaphthylene- 3,8-dione dihydrochloride (Example 13A(k) or 15(d)) 6,7-Dihydro-5Hthieno[ 3,2-fe]pyran-2- carbaldehyde (for a synthesis see WO2007122258 Example 88(c)) (l£)-l-[(4-Arnino-lpiperidinyl) methyl]-l ,2- dihydro-4i/,9Himidazo[ 1,2,3-y]-1,8- naphthyridine-4,9-dione 75 76 77 Di-HCl MS (ES+) m/z 492(MH+) Di-HCl MS (ES+) m/z 463(MH+) Free base MS (ES+) m/z464(MH+) r-rO-trcD xcr dihydrochloride (commercial) (l£)-l-[(4-Amino-lpiperidinyl) methyl]-l ,2- dihydro-4H,9Hrmidazo[ 1,2,3-H]-1,8- naphthyridine-4,9-dione dihydrochloride (Example 5A(j)) 4-Oxo-2,3,4,5- tetrahydro-1,5- benzothiazepine-7- carbaldehyde (for a synthesis see WO2004058144 Example 128(e) (1R)-l-[(4-Amino-lpiperidinyl) methyl]-l,2- dihydro-4H,9Himidazofl ,2,3-ij]-1,8- naphthyridine-4,9-dione dihydrochloride (Example 5A(j)) 8-Methyl-2,3-dihydro- 1,4-benzodioxin-6- carbaldehyde (Prepared from 8-bromo- 2,3-dihydro-l,4- benzodioxin-6- carbaldehyde (for a synthesis see WO2007122258 Example 31(c)) by palladium catalysed Stille coupling with tetramethyltin) (lJ?>l-[(4-Amino-lpiperidinyl) methyl]-1,2- uiliyuiu-4ri,y/7- imidazo[l,2,3-y]-l,8- naphthyridine-4,9-dione 78 79 Di-HCl MS (ES+) m/z 478(MH+) Di-HCl MS (ES+) m/z 466(MH+) H (Formula revomed) dihydrochloride (Example 5A(j)) 3,4-Dihydro-2H-L4- benzothiazin-6- carbaldehyde (for a synthesis see WO2003087098 Example 214) (1R)-l-[(4-Amino-lpiperidinyl) methyl]-l ,2- dihydro-4H,9Himidazo[ l,2,3-i/]-l,8- naphthyridine-4,9-dione dihydrochloride (Example 5A(j)) 2,3,4,5-Tetrahydro-1,5- benzothiazepin-7- carbaldehyde (prepared from methyl 4-oxo- 2,3,4,5-tetrahydro-l ,5- benzomiazepine-7- carboxylate (for a synthesis see WO2007016610, Preparation 18(c)) by treatment with Borane- THF to give methyl 2,3,4,5-tetrahydro-l,5- benzothiazepine-7- carboxylate, then treatement of this with LiAlft, to give 2,3,4,5- tetrahydro-1,5- benzothiazepin-7- ylmethanol and finally treatment with MnCh (l£>l-[(4-Amino-lpiperidinyl) methyl]-1,2- dmydro-4fl,9Himidazo[ l,2,3-y]-l,8- naphthyridine-4,9-dione -122- dihydrochloride (Example 5A(j)) 7-Fluoro-3,4-dihydro- 2H-1,4-benzoxazine-6- carbaldehyde (prepared from 7-fluoro-3-oxo-3,4- dihydro-2H-l,4- benzoxazine-6- carbonitrile (for a synthesis see WO2002056882, Example 8(b)) by treatment with diisobutylaluminium hydride) Preparation A: 6,7-Dihydro[l,4]dioxino[2,3-c]pyridazine-3-carbaldehyde (a) 3,4,6-Trichloropyridazine This was prepared by a slight variation on the method of Kasnar et ah Nucleosides & Nucleotides (1994), 13(1-3), 459-79. Hydrazine sulphate salt (51 g) was suspended in water (250ml), heated to reflux and bromomaleic anhydride (90.38 g) was added dropwise. The mixture was heated at reflux for 4 hours then cooled to room temperature. The reaction was repeated with 29g hydrazine sulphate, 53g bromomaleic anhydride and 130ml water. The precipitates were collected by filtration, washed with water and acetone and dried as a combined batch in vacuo to afford 4-bromo-l,2-dihydro-3,6-pyridazinedione as a white solid (113 g). The solid in two batches was treated with phosphorus oxychloride (2x200 ml) and heated to reflux for 3.5 hours. The mixture was cooled, evaporated and azeotroped with toluene. The residue was partitioned between dichloromethane and saturated aqueous sodium bicarbonate solution and extracted with DCM twice more. The organic extracts were dried and evaporated. This residue was re-dissolved in dichloromethane, and chromatographed on silica gel (300 g) (DCM as eluent) to give a white solid (101.5 g, 87%). (LC/MS analysis showed ca 20-30% impurity, isomers of bromo-dichloropyridazine). MS (+ve ion electrospray) m/z 184/185/186 (MH+), trichloropyridazine. MS (+ve ion electrospray) m/z 228/229/231 (MH+), bromo-dichloropyridazine. (b)2-[(3,6-Dichloro-4-pyridazinyl)oxy]ethanol A solution of ethylene glycol (55 ml) in tetrahydrofuran (200 ml) was treated at around 0°C (ice bath cooling) with sodium hydride (60% dispersion in oil, 5.9 g) over 40 minutes. After the addition was complete, 3,4,6-trichloropyridazine (27 g) containing isomers of bromo-dichloropyridazine as impurity was added portionwise and washed in with more dry THF (50ml) and the mixture was stirred at 0°C for 1 hour and then at room -123- temperature overnight. The mixture was concentrated (to 1/3 volume) then diluted with aqueous sodium bicarbonate solution and extracted with chloroform (5x) and ethyl acetate (3x). The combined organic extracts were washed with water, dried over sodium sulphate and evaporated and the solids filtered off and washed with CHCI3 (x3) and dried in a vacuum oven overnight at 40°C affording a white solid (25.5 g, 83%), containing some bromo-derivative (10-15%). MS (+ve ion electrospray) m/z 209/211 (MH+). MS (+ve ion electrospray) m/z 255/7 (MH+), bromo-derivative. (c)3-Chloro-6,7-dihydro[l,4]dioxino[2,3-c]pyridazine A solution of 2-[(3,6-dichloro-4-pyridazinyl)oxy]ethanoI containing some bromoderivative (15.46 g; 0.0703 mol) in dry 1,4-dioxane (1.2 L) was treated with lithium hydride (2.3 g; 0.28 mol) in portions and stirred at room temperature for 1 hour under argon, then heated at 110 °C overnight. The reaction mixture was quenched with wet 1,4- dioxane, then iced-water. The solution was evaporated to half volume, taken to pH 8 with 5M hydrochloric acid and evaporated to dryness. Water was added and the residue was extracted 5x with chloroform, dried (sodium sulphate) and evaporated to afford a white solid (12.4 g, ca.77%) (containing ca. 15% of a bromo species). MS (+ve ion electrospray) m/z 173/5 (CI MH+); 217/9 (Br MH+) (d) 3-Ethenyl-6,7-dihydro[l ,4]dioxino[2,3-c]pyridazine A solution of 3-chloro-6,7-dihydro[l,4]dioxino[2,3-c]pyridazine (13.6 g, 0.079 mol) containing ca. 15% of a bromo species in dimethoxyethane (400 ml) was degassed under argon for 10 min then tetrakis(triphenylphosphine)palladium (0) (2 g), potassium carbonate (10.33 g), 2,4,6-trivinylcyclotriboroxane pyridine complex (11.32 g) and water (55 ml) were added. The mixture was heated at 95 °C for 48 hours and cooled and evaporated to dryness. The mixture was treated with aqueous sodium bicarbonate solution and extracted (5x) with DCM. Extracts were dried (sodium sulphate), evaporated and the residue chromatographed on silica gel (500 g), eluting with 0-100% ethyl acetate - hexane, affording the product (6.43 g, 50%); [also some impure fractions (1.8 g)]. MS (+ve ion electrospray) m/z 165 (MH+). (e) Title compound A solution of 3-ethenyl-6,7-dihydro[l,4]dioxino[2,3-c]pyridazine (11.58 g) in 1,4- dioxane/water (600 ml/180 ml), cooled in ice, was treated with an aqueous solution of osmium tetroxide (4% w/v, 25 ml) and sodium periodate (43 g). This mixture was allowed to warm to room temperature and after 7 hours under stirring the mixture was evaporated to dryness and azeotroped with 1,4-dioxane. Silica gel, 1,4-dioxane and chloroform were added and the mixture was evaporated to dryness overnight, then added to a silica column (400 g) and chromatographed, eluting with chloroform then 0-100% ethyl acetate in hexane, to afford a white solid (7.55 g, 64%). MS (+ve ion electrospray) m/z 167 (MH+). Biological Activity Antimicrobial Activity Assay: Whole-cell antimicrobial activity was determined by broth microdilution using the Clinical and Laboratory Standards Institute (CLSI) recommended procedure, Document M7-A7, "Methods for Dilution Susceptibility Tests for Bacteria that Grow Aerobically". The compounds were tested in serial two-fold dilutions ranging from 0.016 to 16 mcg/ml. The minimum inhibitory concentration (MIC) was determined as the lowest concentration of compound that inhibited visible growth. A mirror reader was used to assist in determining the MIC endpoint. Compounds were evaluated against Gram-positive organisms, selected from Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Enterococcus faecalis and Enterococcus faecium. In addition, compounds were evaluated against Gram-negative organisms selected from Haemophilus influenzae, Moraxella catarrhalis, Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Enterobacter cloacae, Enterobacter aerogenes, Klebsiella pneumoniae and Stenotrophomonas maltophilia. Each of the listed Examples, as identified in the present application, except Examples 71-73 and 76-79, was tested in at least one exemplified salt or free base form. Unless otherwise noted, the tested Examples had a MIC least one of the organisms listed above, with the exception of Example 9 which had an MIC one strain of every organism listed above, at least one Example had a MIC Mycobacterium tuberculosis H37Rv Inhibition Assay The measurement of the minimum inhibitory concentration (MIC) for each tested compound was performed in 96 wells flat-bottom, polystyrene microtiter plates. Ten twofold drug dilutions in neat DMSO starting at 400uM were performed. Five ul of these drug solutions were added to 95 ul of Middlebrook 7H9 medium. (Lines A-H, rows 1-10 of the plate layout). Isoniazid was used as a positive control, 8 two-fold dilution of Isoniazid starting at 160 µgml-1 was prepared and 5 µl of mis control curve was added to 95 ul of Middlebrook 7H9 (Difco catalogue Ref. 271310) + ADC medium (Becton Dickinson Catalogue Ref. 211887). (Row 11, lines A-H). Five ul of neat DMSO were added to row 12 (growth and Blank controls). The inoculum was standardised to approximately 1x107 cfu/ml and diluted 1 in 100 in Middlebrook 7H9+ADC medium and 0.025% Tween 80 (Sigma P4780), to produce the final inoculum of H37Rv strain (ATCC25618). One hundred ul of this inoculum was added to the entire plate but G-12 and H-12 wells (Blank controls). All plates were placed in a sealed box to prevent drying out of the peripheral wells and they were incubated at 37°C without shaking for six days. A resazurin solution was prepared by dissolving one tablet of resazunn (Resazurin Tablets for Milk Testing; Ref 330884Y VWR International Ltd) in 30 ml sterile PBS (phosphate buffered saline). 25 ul of this solution was added to each well. Fluorescence was measured (Spectramax M5 Molecular Devices, Excitation 530nm, Emission 590nm) after 48 hours to determine the MIC value. Examples 1-4, 5A, 6A, 7-9,12,13A, 14,15,16A, 17A, 19A, 20, 23-32, 34, 37- 44, 46-50, 53-57, 59 and 63 were tested in the Mycobacterium tuberculosis H37Rv inhibition assay. Examples 2, 4, 5A, 6A, 7, 8,12, 13A, 14, 16A, 19A, 20, 23-26, 28-32, 34, 37, 39,40, 42,44,46, 49, 50, 56, 59 and 63 showed an MIC value of lower than 2.0 µg/ml. Examples 4, 5A, 7, 8,12,13A, 14,16A, 19A, 25, 26, 30-32, 37, 40,49, 50, and 56 showed an MIC value of 1.0 ug/ml or lower. Claims 1. A compound of formula (I) or a pharmaceutically acceptable salt and/or N-oxide thereof: A—NR2—UR5 0 O ^ N ^ N ^ ?1 b - ^ Z 1^TVa (I) wherein: Z1 and Z2 are independently selected from CH and N; R1a and R1b are independently selected from hydrogen; halogen; cyano; (C1-6)alkyl; (C1-6)alkylthio; trifluoromethyl; trifiuoromethoxy; carboxy; hydroxy optionally substituted with (C1-5)alkyl or (C1-6)alkoxy-substiruted(C 1-6)alkyl; (C1-6)alkoxysubstituted( C1-6)alkyl; hydroxy (C1-6)alkyl; an amino group optionally N-substituted by one or two (C1-6alkyl, formyl, (C1-6)alkylcarbonyl or (C1-6)alkylsulphonyl groups; and aminocarbonyl wherein the amino group is optionally substituted by (C1-6)alkyl; provided that R1a and R1b are H when Z2 or Z1 is N, respectively, R2 is hydrogen, or (C1-4)alkyl, or together with R6 forms Y as defined below; A is a group (i): R3 M, 'On (ia) or (ib) in which: R3 is as defined for R1a and R1b or is oxo and n is 1 or 2: or A is a group (ii) 127 Wf^VpCH2 N ' (ii) W1, W2 and W3 are CR4R8 or W2 and W3 are CR4R8 and W1 represents a bond between W3 and N. X is O,CR4R8,orNR6; one R4 is as defined for R1a and R1b and the remainder and R8 are hydrogen or one R4andR8 are together oxo and the remainder are hydrogen; R6 is hydrogen or (C1-6)alkyl; or together with R2 forms Y; R7 is hydrogen; halogen; hydroxy optionally substituted with (C1-6alkyl; or (C1- 6)alkyl; Y is CR4R8CH2; CH2CR4R8; (C=O); CR4R8; CR4R8(C=O); or (C=O)CR4R8; or when X is CR4R8, R8 and R7 together represent a bond; U is selected from CO, and CH2 and R5 is an optionally substituted bicyclic carbocyclic or heterocyclic ring system (B): (a) | containing up to four heteroatoms in each ring in which at least one of rings (a)and (b) is aromatic; X1 is C or N when part of an aromatic ring, or CR14 when part of a non-aromatic ring; X2 is N, NR13, O, S(O)x, CO or CR14 when part of an aromatic or non-aromatic ring or may in addition be CR14R15 when part of a non aromatic ring; X3andX5 are independently N or C; Yl is a 0 to 4 atom linker group each atom of which is independently selected from N, NR13, O, S(O)X, CO and CR14 when part of an aromatic or non-aromatic ring or may additionally be CR14R15 when part of a non aromatic ring; Y2 is a 2 to 6 atom linker group, each atom of Y2 being independently selected from N, NR13,O, S(O)X, CO, CR14 when part of an aromatic or non-aromatic ring or may additionally be CR14R15 when part of a non aromatic ring; each of R14 and R15 is independently selected from: H; (C1-4)alkylthio; halo; carboxy(C1-4)alkyl; (C1-4)alkyl; (C1-4)alkoxycarbonyl; (C1-4)alkylcarbonyl; (C1- -128- 4)alkoxy(C1-4)alkyl; hydroxy; hydroxy(C1-4)alkyl; (C1-4)alkoxy; nitro; cyano; carboxy; amino or aminocarbonyl optionally mono- or di-substituted by (C1-4)alkyl; or R14andR15 may together represent oxo; each R13 is independently H; trifluoromethyl; (C1-4)alkyl optionally substituted by hydroxy, (C1-6)alkoxy, (C1-6)alkylthio, halo or trifluoromethyl; (C2-4)alkenyl; (C1- 4)alkoxycarbonyl; (C1-4)alkylcarbonyl; (C1-6)alkylsulphonyl; aminocarbonyl wherein the amino group is optionally mono or disubstituted by (C1-4)alkyl; and each x is independently 0,1 or 2. 2. A compound according to claim 1 wherein: (i) Z1 and Z2 are both CH; (ii) Z1 is N and Z2 is CH;or (iii) Zl is CH and Z2 is N. 3. A compound according to any preceding claim wherein R1a is hydrogen and R1b is hydrogen. 4. A compound according to any preceding claim wherein A is (ia), n is 1 and R3 is H or hydroxy in the 3-position, A is (ii), X is CR4R8 and R8 is H and R4 is H or OH, or A is (ii), X is O, R7 is H and W1, W2 and W3 are each CH2. 5. A compound according to claim 4 wherein A is piperidin-4-yl or pyrrolidin-4- ylmethyl. 6. A compound according to any preceding claim wherein U is CH2. 7. A compound according to any preceding claim wherein R5 is an aromatic heterocyclic ring (B) having 8-11 ring atoms including 2-4 heteroatoms of which at least one is N or NRl 3 in which Y2 contains 2-3 heteroatoms, one of which is S and 1 -2 are N, with one N bonded to X3, or the heterocyclic ring (B) has ring (a) aromatic selected from optionally substituted benzo, pyrido, pyridazino and pyrimidino and ring (b) non aromatic and Y2 has 3-5 atoms, including at least one heteroatom, with O, S, CH2 or NR13 bonded to X5 where R13 is other than hydrogen, and either NHCO bonded via N to X3, or O, S, CH2 or NH bonded to X3. 8. A compound according to any of claims 1 to 6 wherein R5 is selected from: 3-oxo-3,4-dihydro-2H-pyrido[3,2-b][l,4]oxazin-6-yl 3-oxo-3,4-dihydro-2H-pyrido [3,2-b] [ 1,4]thiazin-6-yl -129- 2,3-dihydro-[l,4]dioxmo[2,3-c]pyridin-7-yl [ 1,3]oxathiolo[5,4-c]pyridin-6-yl 6-fluoro-2,3 -dihydro-1,4-benzodioxin-7 -yl 2,3-dihydro[ 1,4]oxathiino[2,3-c]pyridin-7-yl 3,4-dihydro-2H-pyrano[2,3-c]pyridin-6-yl 5-fluoro-2,3 -dihydro-1,4-benzodioxin-7-yl 5-carbonitro-2,3-dihydro-l,4-benzodioxin-7-yl 2,3-dihydro-benzo[1,4]dioxin-6-yl. 9. A compound according to claim 1 selected from: 1 -{{4-[(2,3 -Dihydro[ 1,4] dioxino[2,3-c]pyridin-7-ylmethyl)amino]-1 -piperidinyl} methyl)- l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione; l-({4-[([13]Oxathiolo[5,4-c]pyridin-6-ylmethyl)amino]-l-piperidinyl}methyl)-l)2- dihydro-4H,9H-imidazo[l,2,3-ij]-l,8-naphthyridine-4,9-dione; l-({4-[(2,3-Dihydro[l,4]dioxino[2,3-c]pyridin-7-ylmethyl)amino]-l-piperidinyl}methyl)- 1,2-dihydro-4H,9H-imidazo[ 1,2,3-ij]-1,8-naphthyridine-4,9-dione; l-( {4-[(2,3-Dihydro[l ,4]dioxino[2,3-c]pyridin-7-ylmethyl)amino]- l-piperidinyl}methyl)- 1,2-dihydro-4H,9H-imidazo[ 1,2,3-ij]-1,8-naphthyridine-4,9-dione; (1R)-1 -({4-[([ 1,3]Oxathiolo [5,4-c]pyridin-6-ylmethyl)amino]-1 -piperidinyl) methyl)-1,2- dihydro-4H,9H-imidazo[ 1,2,3-ij]-1,8-naphthyridine-4,9-dione; (1R)-l-({4-[(23-Dihydro[l,4]oxatniino[2,3-c]pyridin-7-ylmethyl)amino]-lpiperidmyl} methyl)-l,2-dihydro-4H,9H-imidazo[l,2,3-ij]-1,8-naphthyridine-4,9-dione; (lR)-l-({4-[(5,6,7,8-TetTahydro-3-isoquinolinylmethyl)amino]-l-piperidinyl}methyl)- 1,2-dihydro-4H,9H-imidazo[l ,2,3-ij]-l ,8-naphthyridine-4,9-dione; (1R)-1-({4-[(6,7-Dihydro-5H-cyclopenta[c]pyridin-3-ylmethyl)amino]-lpiperidinyl} methyl)-l,2-dihydro-4H,9H-imidazo[l,2,3-ij]-l,8-naphthyridine-4,9-dione; (li?)-l-({4-[(l,3-Dihydrofuro[3,4-c]pyridin-6-ylmethyl)amino]-l-piperidinyl}methyl)- 1,2-dihydro-4H,9H-imidazo[ 1,2,3-ij]-1,8-naphthyridine-4,9-dione; (1R)-1 -( {4-[(3,4-Dihydro-2i7-pyrano[2,3-c]pyridin-6-ylmethyl)amino]-l - piperidmyl}methyl)-l,2-dihydro-4H,9H-imidazo[l,2,3-i/]-l,8-naphthyridine-4,9-dione; 7-[( {1 -[(4,9-Dioxo-1,2-dmydro-4H,9H-miidazo[l ,2,3-y]-l ,8-naphthyridin-l -yl)methyl]- 4-piperidinyl} amino)methyl]-2,3-dihydro-1,4-benzodioxin-5-carbonitrile; 1 -[(4- {[(3-Oxo-3,4-dihydro-2H-1,4-benzothiazin-6-yl)methyl]amino} -1 - piperidinyl)methyl]-l ,2-dihydro-4H,9H-imidazo[l ,2,3-ij]-1,8-naphthyridine-4,9-dione; (1R)-1 -({4-[([l ,3]Oxathiolo[5,4-c]pyridin-6-ylmethyI)amino]-1 -piperidinyl }methyl)-1,2- dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione; (1R)-1 -((4-[(2,3-Dihydro[ 1,4]oxathiino[2,3-c]pyridin-7-ylmethyl)amino]-l - piperidinyl}methyl)-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione; (1R)-l-({4-[(2,3-Dihydro[l,4]dioxino[2,3-c]pyridm-7-ylmethyl)amino]-lpiperidinyl} methyl)-1,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione; (2R)-2-({4-[([l,3]Oxamiolo[5,4-c]pyridin-6-ylmethyl)amino]-l-piperidinyl}methyl)-l,2- dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione; -130- (2S)-2-( {4-[([ 1,3 ]Oxathiolo[5,4-c]pyridin-6-ylmethyl)amino]-1 -piperidinyl) methyl)-1,2- dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione; 2-( (4-[([1 ,3]Oxathiolo[5,4-c]pyridin-6-ylmethyl)amino]-l -piperidinyl} methyl)-1,2- dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione; (2R)-2-({4-[(2,3-Dihydro[l,4]oxathiino[2,3-c]pyridin-7-ylmethyl)amino]-lpiperidinyl} methyl)-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione; 2-({4-[(2,3-Dihydro[l,4]oxathiino[2,3-c]pyridin-7-ylmethyl)amino]-1- piperidinyl}methyl)-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione; (2S)-2-({4-[(2,3-Dihydro[l,4]oxamimo[2,3-c]pyridin-7-ylmethyl)amino]-lpiperidinyl} methyl)-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione; 2-({4-[(2,3-Dihydro[l,4]dioxino[2,3-c]pyridin-7-ylmethyl)amino]-l-piperidinyl}methyl)- l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione; (1R)-1-({4-[(2,3-Dihydro[l,4]oxathiino[2,3-c]pyridin-7-ylmethyl)amino]-4-methyl-lpiperidinyl} methyl)-1,2-dihydro-4H,9H-imidazo[ 1,2,3-y]-l ,8-naphthyridine-4,9-dione; (1R)-1 -({4-Methyl-4-[([ 1,3]oxathiolo[5,4-c]pyridin-6-ylmethyl)amino]-1 - piperidmyl}methyl)-l,2-dihydro-4H,9H-imidazo[l,2,3-y]-1,8-naphthyridine-4,9-dione; (2R)-2-({4-[(2,l,3-Benzothiadiazol-5-ylmethyl)amino]-l-piperidinyl}methyl)-l,2- dihydro-3R,8H-2a,5,8a-triazaacenaphthylene-3,8-dione; (2R)-2-[(4- {[(7-Fluoro-2,3 -dihydro-1,4-benzodioxin-6-yl)methyl]amino} -1 - piperidinyl)methyl]-1,2-dmydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione; (2R)-2-({4-[(3,4-Dihydro-2H-[l,4]oxathiepino[2,3-c]pyridin-8-ylmethyl)amino]-lpiperidinyl} methyl)-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione; (2R)-2-({4-[([1 ,3]Oxathiolo[4,5-c]pyridin-6-ylmethyl)amino]-1 -piperidinyl}methyl)-1,2- dihycho-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione; (2R)-2-[(4-{[(3-Oxo-3,4-dihydro-2H-pyrido[3,2-6][l,4]thiazin-6-yl)methyl]amino}-lpiperidinyl) methyl]-1,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione; (1R)-1 -({4-[(2,3 -Dihydro-1,4-benzodioxin-6-ylmethyl)amino] -1 -piperidinyl) methyl)-1,2- dihydro-4H,9H-imidazo[l ,2,3-y]-l ,8-naphthyridine-4,9-dione; (1R)-1 -[(4- {[(8-Fluoro-2,3 -dihydro-1,4-benzodioxin-6-yl)methyl]amino} -1 - piperidinyl)methyl]-l ,2-dihydro-4H,9H-imidazo[l ,2,3-y]-l ,8-naphthyridine-4,9-dione; (lR)-l-[(4-{[(7-Chloro-3-oxo-3,4-dihydro-2H-pyrido[3,2-6][l)4]oxazin-6- yl)methyl]amino}-1 -piperidinyl)methyl]-1,2-dihydro-4H,9H-imidazo[ 1,2,3-ij ]-1,8- naphthyridine-4,9-dione; (1R)-l-[(4-{[(4-Chloro-7-oxo-6,7-dihydro-li/-pyrimido[5,4-6][l,4]oxazin-2- yl)methyl] amino}-1 -piperidinyl)methyl]-l ,2-dihydro-4H,9H-imidazo[l ,2,3-y]-1 ,8- naphthyridine-4,9-dione; (lR)-1-[(4-{[(7-Oxo-6,7-dihydro-1H-pyrimido[5,4-6][l,4]thiazin-2-yl)methyl]amino}-lpiperidinyl) methyl]-l,2-dihydro-4H,9H-imidazo[l,2,3-ij]-l,8-naphthyridine-4,9-dione; (IR)-1 -({4-[(l ,2,3-Benzothiadiazol-5-ylmethyl)amino]- l-piperidinyl}methyl)-l ,2- dihydro-4H,9H-imidazo[l,2,3-ij]-\ ,8-naphthyridine-4,9-dione; (1R)-1 -({4-[(2,3 -Dihydro-1 -benzofixran-5-ylmethyl)amino]-1 -piperidinyl} methyl)-1,2 - dihydro-4H,9H-imidazo[l,2,3-ij]-l,8-naphthyridine-4,9-dione; -131- (1R)-1 -({4-[(3,4-Dihydro-2H-pyrano[2,3-c]pyridin-6-ylmethyl)amino]-1 - piperidinyl}methyl)-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione; (1R)-1 -({4-[(2,3-Dihydrofuro[2,3 -c]pyridin-5-ylmethyl)amino]-1 -piperidinyl} methyl)- l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione; (2R)-2-({4-[(3,4-Dihydro-2H-pyrano[2,3-c]pyridin-6-ylmethyl)amino]-1- piperidinyl}methyl)-l,2-dihydro-3i/,8i/-2a,5,8a-triazaacenaphthylene-3,8-dione; (2R)-2-({4-[(2,3-Dihydro-l,4-benzodioxin-6-ylmethyl)amino]-l-piperidmyl}methyl)-l,2- dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione; (2R)-2-[(4- {[(8-Fluoro-2,3-dihydro-1,4-benzodioxin-6-yl)methjyl] amino} -1 - piperidinyl)methyl]-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione; 7-{[(1 -{[(2R)-3,8-Dioxo-1,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylen-2-yl]methyl} - 4-piperidinyl)amino]methyl} -2,3-dihydro-1,4-benzodioxin-5-carbonitrile; (2R)-2-({4-[(2,3-Dihydrofuro[2,3-c]pyridin-5-ylmethyl)aniino]-l-piperidinyl}methyl)- 1,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione; (1R)-1 -({4-[(2,3-Dihydro-1,4-benzodioxin-6-ylmethyl)amino]-1 -piperidinyl} methyl)-1,2- dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione; (1R)-l-({4-[([l,2,5]Thiadiazolo[3,4-6]pyridin-6-ybnethyl)amino]-l-piperidinyl} methyl)- 1,2-dihydro-4H,9H-imidazo[ 1,2,3-ij]-1,8-naphthyridine-4,9-dione; (1R)-1 -[(4- {[(4-Fluoro-1H-benzimidazol-2-yl)methyl]amino} -1 -piperidinyl)methyl]-l ,2- dihydro-4H,9H-imidazo[l ,2,3-ij]-l ,8-naphthyridine-4,9-dione; (1R)-1 -[((2S)-2- {[([ 1,3]Oxathiolo[5,4-c]pyridin-6-ylmethyl)amino]methyl} -4- morpholinyl)methyl]-1,2-dihydro-4H,9H-imidazo[ 1,2,3-ij]-1,8-naphthyridine-4,9-dione; (lR)-l-{[(2S)-2-({[(7-Chloro-3-oxo-3,4-dihydro-2H-pyrido[3,2-6][l,4]oxazin-6- yl)methyl]amino}methyl)-4-morpholinyl]methyl}-l,2-dihydro-4H,9H-imidazo[l,2,3-ij]- 1,8-naphthyridine-4,9-dione; (2R)-2-( {4-[([ 1,2,5]Thiadiazolo[3,4-6]pyridin'6-ytaiethyl)amino]-1 -piperidinyl} methyl)- l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione; (1R)-l-({4-[(3,4-Dihydro-2H-chromen-7-ylmethyl)amino]-l-piperidinyl}methyl)-l,2- dihydro-4H,9H-imidazo[ 1,2,3-y]-1 ,8-naphthyridine-4,9-dione; (1 R)~ 1 -({4-[(2,3-Dihydro-1 -benzofuran-6-ylmethyl)amino]-1 -piperidinyl} methyl)- 1,2- dihydro-4H,9H-imidazo[l ,2,3-y]-l ,8-naphthyridine-4,9-dione; (lR)-l-({4-[(3,4-Dihydro-2H-chromen-6-ylmethyl)amino]-l-piperidinyl}methyI)-l,2- dihydro-4H,9H-imidazo[l ,2,3-ij]-l ,8-naphthyridine-4,9-dione; (2R)-2-[(4-{[(5-Fluoro-2,3-dihydro-l,4-benzodioxin-6-yl)methyl]amino}-lpiperidmyl) methyl]-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione; (lR)-1-{[(2,S)-2-({[(7-fluoro-2,3-dihydro-l,4-benzodioxin-6-yl)methyl]amino}methyl)-4- morpholinyl]methyl} -1,2-dihydro-4H,9H-imidazo[l ,2,3-y]-1,8-naphthyridine-4,9-dione; (1R)-1 -[((35)-3- {[([1,3]Oxathiolo[5,4-c]pyridin-6-ylmethyl)amino]methyl} -1 - pyrrolidinyl)methyl]-l,2-dihydro-4H,9H-imidazo[l,2,3-i/]-l,8-naphthyridine-4,9-dione; 7-{[(l-{[(1R)-3,8-Dioxo-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylen-l-yl]methyl}- 4-piperidinyl)amino]methyl} -2,3 -dihydro-1,4-benzodioxin-5 -carbonitrile; (1R)-1 -[(4- {[(7-Fluoro-2,3-dihydro-1,4-benzodioxin-6-yl)methyl]amino} -1- piperidinyl)methyl] -1,2-dihydro-3H,8H-2 a,5, 8a-triazaacenaphthylene-3,8-dione; -132- (1R)-l-[(4-{[(8-Fluoro-2,3-dihydro-l,4-benzodioxin-6-yl)methyl]amino}-1- piperidinyl)methyl]-l,2-dihydro-3H,8H-2a,5,8a-triazaacenaphthylene-3,8-dione; (1R)-l -[(4- {[(2-Oxo-2H-chromen-7-yl)methyl]amino} -1 -piperidinyl)methyl]-1,2- dihydro-4H,9H-imidazo[1,2,3-ij]-1,8-naphthyridine-4,9-dione; (1R)-l-[(4-{[(2-oxo-2H-clromen-7-yl)methyl]amino}4-piperidbyl)methyl]-l,2-dihydro- 4H,9H-imidazo[l ,2,3-ij]-l ,8-naphthyridine-4,9-dione; (IR)-1 -[(4- {[(2-oxo-2H-chromen-7-yl)methyl]amino} -1 -piperidinyl)methyl]-1,2-dihydro- 4H,9H-imidazo[l,2,3-ij]-l,8-naphthyridiiie-4,9-dione; (1R)-l methyl)-1 ,2-dihydro-4H,9H-imidazo[1 ,2,3-ij]-l ,8-naphthyridine-4,9-dione; orl -[(4- {[(3-Oxo-3,4-dihydro-2i7-1,4-benzottaazm-6-yl)memyl]amino} -1 - piperidmyl)memyl]-l,2-dmydro-4J7,9i7-imidazo[1,2,3-ij]-1,8-naphthyridine-4,9-dione; or a free base of a compound of Table 1; or a pharmaceutically acceptable salt thereof. 10. A compound according to any of claims 1 to 9, for use in therapy. 11. A compound according to any of claims 1 to 9, for use in the; treatment of bacterial infections in mammals. l2. A pharmaceutical composition comprising a compound according to any of claims 1 to 9 and a pharmaceutically acceptable carrier. 13. A compound of formula (IIIA): N. / N N ^ 0 (IIIA) formula (IIIB): -133- or formula (8): N. J> R1a (1MB) (8) in which L is -A-N(R20)R2' AND R20 is hydrogen, R2' is R2 or a group convertible thereto and A, R2, R1a and R1b are as defined in claim 1. |
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| Patent Number | 279599 | ||||||||||||||||||
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| Indian Patent Application Number | 6204/DELNP/2009 | ||||||||||||||||||
| PG Journal Number | 05/2017 | ||||||||||||||||||
| Publication Date | 03-Feb-2017 | ||||||||||||||||||
| Grant Date | 27-Jan-2017 | ||||||||||||||||||
| Date of Filing | 29-Sep-2009 | ||||||||||||||||||
| Name of Patentee | GLAXO GROUP LIMITED | ||||||||||||||||||
| Applicant Address | GLAXO WELLCOME HOUSE, BERKELEY AVENUE, GREENFORD, MIDDLESEX UB6 0NN, U.K | ||||||||||||||||||
Inventors:
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| PCT International Classification Number | A61K 31/4985 | ||||||||||||||||||
| PCT International Application Number | PCT/EP2008/054621 | ||||||||||||||||||
| PCT International Filing date | 2008-04-16 | ||||||||||||||||||
PCT Conventions:
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