Title of Invention	DNA ENCODING RING ZINC-FINGER PROTEIN AND THE USE OF THE DNA IN VECTORS AND BACTERIA AND IN PLANTS
Abstract	ABSTRACT DNA ENCODING RING ZINE-FINGER PROTEIN AND THE USE OF THE DNA IN VECTORS AND BACTERIA AND IN PLANTS The present inventions relate to compositions and methods for providing stress tolerant transgenic plants comprising a RING domain zinc-finger motif transcription factor protein. More particularly, the invention relates to compositions and methods comprising a RING-H2 domain transcription factor protein for providing drought and salt tolerant plants, in particular comprising a recombinant XERICO gene and protein.

Title of Invention

DNA ENCODING RING ZINC-FINGER PROTEIN AND THE USE OF THE DNA IN VECTORS AND BACTERIA AND IN PLANTS

Abstract

ABSTRACT DNA ENCODING RING ZINE-FINGER PROTEIN AND THE USE OF THE DNA IN VECTORS AND BACTERIA AND IN PLANTS The present inventions relate to compositions and methods for providing stress tolerant transgenic plants comprising a RING domain zinc-finger motif transcription factor protein. More particularly, the invention relates to compositions and methods comprising a RING-H2 domain transcription factor protein for providing drought and salt tolerant plants, in particular comprising a recombinant XERICO gene and protein.

Full Text	CLAIMS We claim; 1. An expression vector construct comprising a nucleic acid encoding a polypeptide at least 32% identical to SEQ ID NO;01, operably linked to a heterologous promoter, wherein said nucleic acid encodes a polypeptide comprising a RING-H2 zinc-finger motif transcription domain, a low complexity region, and a transmembrane domain for increasing tolerance to abiotic stress in a plant. 2. The expression vector construct of claim 1, wherein said RING-H2 zinc-finger motif transcription domain comprises SEQ ID NO;06 or SEQ ID NO:07. 3. The expression vector construct of claim 2, wherein said RING-H2 zinc-finger motif transcription domain has at least 40% amino acid sequence identity to SEQ ID NO:03. 4. The expression vector construct of claim 1, wherein said low complexity region is selected from one or more of the group SEQ ID NO:376-397. 5. The expression vector construct of claim 1, wherein said transmembrane region consists of one or more of the group SEQ ID NO:09. 6. The expression vector construct of claim 5, wherein said transmembrane region consists of one or more of the group SEQ ID NOs: 478-492. 7. The expression vector construct of claim I, wherein said polypeptide binds to a COP 1-INTERACTING PROTEIN 8 SEQ ID NO:37g or a TUBBY-like protein 9 SEQ ID NO;380. 8. The expression vector construct of claim 1, wherein said abiotic stress is one or more of drought tolerance and salt tolerance. 9. The expression vector construct of claim 1, wherein said polypeptide is at least 56% identical to SEQ ID NO.01. 10. The expression vector construct of claim 9, wherein said polypeptide is a full-length polypeptide. 11. The expression vector construct of claim I, wherein said heterologous promoter is a promoter operable in a plant. 12. The expression vector construct of claim I, wherein said heterologous promoter is a promoter operable in yeast 13. A transgenic plant comprising a heterologous nucleic acid molecule encoding a polypeptide that is at least 32% identical to SEQ ID NO:01, wherein said polypeptide comprises a RING-H2 zinc-finger motif transcription domain, a low complexity region, and a transmembrane domain, for increasing tolerance to abiotic stress in a plant, for increasing tolerance to abiotic stress in a plant. 14. The transgenic plant of Claim 13, wherein said heterologous nucleic acid molecule is contained within the expression vector of Claim 1. 15. The transgenic plant of Claim 14, wherein said plant is selected from the group consisting ofizoiaceoe, Amaranthaceae, Alliaceae. Asteraceae, Brassicaceae, Cucurbitaceae, Euphorbiaceae, Fabaceae, Malvaceae, Pinaceae, Poaceae, Rosaceae, Solanaceae, Salicaceae, Scrophulariaceae, and Viiaceae. 16. The transgenic plant of claim 15, wherein said plant is selected from the group consisting of a crop plant, an herb plant, a turfgrass plant, an ornamental plant and a tree. 17. The transgenic plant of claim 16, wherein said plant is selected from the group consisting of Arabidopsis sp., Capsicum sp., CitruUus sp., Fragaria sp,. Glycine sp., Gossypium sp,, Heliantkus sp,, Hevea sp., Hordeum sp., Lactuca sp., Lycopersicon sp., Lotus sp. Oryza sp., Pinus sp., Picea sp., Poncim sp., Solarium sp., Sorghum sp., Thellungieila sp., Triphysaria sp,, Triticum sp., Vaccinium sp., and Zea species. 18. A method for altering the phenotype of a plant, comprising; a) providing; i) an expression vector construct comprising a nucleic acid sequence encoding a polypeptide at least 32% identical to SEQ ID NO:01, wherein said polypeptide comprises a RING-H2 zinc-finger motif transcription domain, a low complexity region, and a transmembrane domain, operably linked to a heterologous promoter, and ii) plant tissue; and b) transfecting the plant tissue with the vector so that the phenotype of a plant derived from said plant tissue is altered, 19. The method of claim 18, wherein said altered phenotype is altered environmental tolerance. 20. The method of claim 19, wherein said altered environmental tolerance is altered abiotic stress.

Full Text

CLAIMS We claim;
1. An expression vector construct comprising a nucleic acid encoding a polypeptide at least 32% identical to SEQ ID NO;01, operably linked to a heterologous promoter, wherein said nucleic acid encodes a polypeptide comprising a RING-H2 zinc-finger motif transcription domain, a low complexity region, and a transmembrane domain for increasing tolerance to abiotic stress in a plant.
2. The expression vector construct of claim 1, wherein said RING-H2 zinc-finger motif transcription domain comprises SEQ ID NO;06 or SEQ ID NO:07.
3. The expression vector construct of claim 2, wherein said RING-H2 zinc-finger motif transcription domain has at least 40% amino acid sequence identity to SEQ ID NO:03.
4. The expression vector construct of claim 1, wherein said low complexity region is selected from one or more of the group SEQ ID NO:376-397.
5. The expression vector construct of claim 1, wherein said transmembrane region consists of one or more of the group SEQ ID NO:09.
6. The expression vector construct of claim 5, wherein said transmembrane region consists of one or more of the group SEQ ID NOs: 478-492.
7. The expression vector construct of claim I, wherein said polypeptide binds to a COP 1-INTERACTING PROTEIN 8 SEQ ID NO:37g or a TUBBY-like protein 9 SEQ ID
NO;380.
8. The expression vector construct of claim 1, wherein said abiotic stress is one or more of drought tolerance and salt tolerance.
9. The expression vector construct of claim 1, wherein said polypeptide is at least 56% identical to SEQ ID NO.01.

10. The expression vector construct of claim 9, wherein said polypeptide is a full-length polypeptide.
11. The expression vector construct of claim I, wherein said heterologous promoter is a promoter operable in a plant.
12. The expression vector construct of claim I, wherein said heterologous promoter is a promoter operable in yeast
13. A transgenic plant comprising a heterologous nucleic acid molecule encoding a polypeptide that is at least 32% identical to SEQ ID NO:01, wherein said polypeptide comprises a RING-H2 zinc-finger motif transcription domain, a low complexity region, and a transmembrane domain, for increasing tolerance to abiotic stress in a plant, for increasing tolerance to abiotic stress in a plant.
14. The transgenic plant of Claim 13, wherein said heterologous nucleic acid molecule is contained within the expression vector of Claim 1.
15. The transgenic plant of Claim 14, wherein said plant is selected from the group consisting ofizoiaceoe, Amaranthaceae, Alliaceae. Asteraceae, Brassicaceae, Cucurbitaceae, Euphorbiaceae, Fabaceae, Malvaceae, Pinaceae, Poaceae, Rosaceae, Solanaceae, Salicaceae, Scrophulariaceae, and Viiaceae.
16. The transgenic plant of claim 15, wherein said plant is selected from the group consisting of a crop plant, an herb plant, a turfgrass plant, an ornamental plant and a tree.
17. The transgenic plant of claim 16, wherein said plant is selected from the group consisting of Arabidopsis sp., Capsicum sp., CitruUus sp., Fragaria sp,. Glycine sp., Gossypium sp,, Heliantkus sp,, Hevea sp., Hordeum sp., Lactuca sp., Lycopersicon sp., Lotus sp. Oryza sp., Pinus sp., Picea sp., Poncim sp., Solarium sp., Sorghum sp., Thellungieila sp., Triphysaria sp,, Triticum sp., Vaccinium sp., and Zea species.

18. A method for altering the phenotype of a plant, comprising;
a) providing;
i) an expression vector construct comprising a nucleic acid sequence
encoding a polypeptide at least 32% identical to SEQ ID NO:01, wherein said polypeptide comprises a RING-H2 zinc-finger motif transcription domain, a low complexity region, and a transmembrane domain, operably linked to a heterologous promoter, and ii) plant tissue; and
b) transfecting the plant tissue with the vector so that the phenotype of a plant
derived from said plant tissue is altered,
19. The method of claim 18, wherein said altered phenotype is altered environmental
tolerance.
20. The method of claim 19, wherein said altered environmental tolerance is altered
abiotic stress.

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Patent Number

279697

Indian Patent Application Number

731/CHENP/2009

PG Journal Number

05/2017

Publication Date

03-Feb-2017

Grant Date

30-Jan-2017

Date of Filing

09-Feb-2009

Name of Patentee

THE BOARD OF TRUSTEES OPERATING

Applicant Address

MICHIGAN STATE UNIVERSITY, 2727 ALLIANCE DRIVE, LANSING, MI 48910

Inventors:

#	Inventor's Name	Inventor's Address
1	HAN, KYUNG-HWAN	1640 BOULEVARD DRIVE OKEMOS, MI 48864
2	KO, JAE-HEUNG	3035-2B TRAPPERS COVE TRAIL LANSING, MI 48910

PCT International Classification Number

C12N15/82

PCT International Application Number

PCT/US2007/015823

PCT International Filing date

2007-07-12

PCT Conventions:

#	PCT Application Number	Date of Convention	Priority Country
1	11/484,947	2006-07-12	U.S.A.