Title of Invention	NOVEL VERBENONE DERIVATIVES PROCESS FOR THEIR PRODUCTION AND PHARMACEUTICAL COMPOSITION
Abstract	The invention discloses the compound having the formula I wherein X, Y and Z are as defined in the specification with the proviso that when when X = O and Y = H, Z is other than H; and, when X = dimethylamino, diethylamino, pyrrolidino, piperidino or morpholino: Y = H; Z = H, CONHAr, CONHR; Ar = aryl, preferably phenyl; R = H, C1-C4 alkyl, C4-C6 cycloalkyl; with the proviso that when when Z = H, X is other than pyrrolidino. The invention is also for process preparation of compounds of the invention, novel intermediates and pharmaceutical composition comprising the compounds of the invention.

Title of Invention

NOVEL VERBENONE DERIVATIVES PROCESS FOR THEIR PRODUCTION AND PHARMACEUTICAL COMPOSITION

Abstract

The invention discloses the compound having the formula I wherein X, Y and Z are as defined in the specification with the proviso that when when X = O and Y = H, Z is other than H; and, when X = dimethylamino, diethylamino, pyrrolidino, piperidino or morpholino: Y = H; Z = H, CONHAr, CONHR; Ar = aryl, preferably phenyl; R = H, C1-C4 alkyl, C4-C6 cycloalkyl; with the proviso that when when Z = H, X is other than pyrrolidino. The invention is also for process preparation of compounds of the invention, novel intermediates and pharmaceutical composition comprising the compounds of the invention.

Full Text	NOVEL VERBENONE DERIVATIVES PROCESS FOR THEIR PRODUCTION AND PHARMACEUTICAL COMPOSITION COMPRISING THEM (i) Verbenone derivatives The present invention relates to novel derivatives of (1S,5S)-4,6,6- trimethylbicyclo[3.1.1]hept-3-en-2-one), which is more commonly known as (-)- verbenone. The oxidation of a- and ß-pinene leads to complex mixtures of monoterpenoid compounds, which are predominantly characterized by alcohol and carbonyl functions; these mixtures have been used for some time, for example in Italy (OZOPULMIN®), France (OZOTHINE®) and Germany (OZOTHIN®), in the therapy of respiratory disorders, owing to their recognised balsamic, bronchosecretolytic and antiseptic properties. Among the above-mentioned monoterpene derivatives of the carbonyl type (-)- verbenone, a ketone derivative having an a-pinane structure whose structural formula is given below, is of particular interest. (-)-verbenone is characterized by its particular pulmonary tropism, its anti- inflammatory action on the respiratory tract and by its good muco-regulatory capacity. The anti-inflammatory activity on the respiratory apparatus has been investigated in a series of classical experimental pharmacology tests: carrageenan- and dextran- induced oedema, adjuvant arthritis (rat), passive cutaneous anaphylaxis (rabbit), turpentine- and carrageenin-induced pleurisy (rat) and acrolein-induced aerosol (guinea pig). GB A-1585 831 and Masataka Watanabe et al. J. Org. Chem; vol. 58, no. 15 (1993) p. 3923-3927 both disclose the compound verbenone which is not in the ambit of the compounds claimed by the present invention. Y. Chretien-Bessiere et al., Comptes Rendus Hebdomadaires des Seances de l'Academie des Sciences, Serie C: Sciences Chimiques, vol.264, no. 8 (1967), p. 710-713) though discloses compounds wherein X in the compounds of the formulae I of the present invention represents pyrrolidino and Y and Z represent H, this possibility has been excluded from the scope of the present invention. Further, the document is silent about the activity or use of the compounds disclosed therein. The present invention solves the problem of oily nature, instability and water- insolubility of (-)- verbenone by preparing derivatives in a solid, water-soluble form while maintaining anti-inflammatory activity in respect of respiratory tract which is typical of (-) verbenone. There is no hint in the prior art that (-) - verbenone derivatives as disclosed in the present invention could solve the problem posed. (-)-verbenone is an oily substance having a characteristic odour and a boiling point of 227-228° C, d= 0.975, no20 = 1.497 and a specific rotatory power which varies according to the respective suppliers ( ACROS catalogue, purity 94%: -193°, c=10, ethanol; FLUKA catalogue, purity = 99%: 180±2°, c=10, ethanol). However, the oily nature, the instability over time and the water-insolubility of (-)- verbenone make it difficult to administer in water-soluble dosage forms. Therefore, the object of the present invention is to synthesize (-)-verbenone derivatives that are in a solid, water-soluble form or which can in any case be readily converted into water-soluble compounds, while maintaining and possibly increasing the anti- inflammatory characteristics in respect of the respiratory tract which are typical of (- )-verbenone. Novel pharmacologically active derivatives of (-)-verbenone having the general formula I given below: wherein, when X = O Z = H, =CHAr, =C(OH)COOEt, =NOR; Y = H, NH2, NH3-X1-, NHCOAr. NHCOR, NHCONHR, NHCONHAr; X1- = pharmaceutically acceptable anion; Ar = aryl or heteroaryl, preferably phenyl, 4-chlorophenyl, 2-furyl, 2-thienyl, 2-hydroxypbenyl, 2-acetoxyphenyl; with the proviso that when X = 0 and Y = H, Z is other than H; ylth the proviso that when Z = H, X is other than pyrrolidino 3. R = H, C1-C4 alkyl, C4-C6 cycloalkyl, CH2COOH, CH2COOEt, CH2COCH3, CH2CN, CH2COCH,COOEt, CH2C6H5; and, when X = dimethylamino, diethylamino, pyrrolidino, piperidino or morpholino: Y = H; Z = H, CONHAr, CONHR; Ar = aryl, preferably phenyl; R=H, C1-C4 alkyl, C4-C6 cycloalkyl have now been found, and form subject-matter of the present invention. The broken line given in the general formula I is thus intended to indicate two distinct structures resulting from the different positioning of the two conjugated double bonds of (-)-verbenone, as is demonstrated more clearly by the general formulae II and III given below: wherein: when Y = H, preferably Z = =CHAr, =C(OH)COOEt, =NOR (that is to say, the Z siroup and the C-10 are joined by a double bond); or, alternatively, when Z = H, preferably Y = NH2, NH3+X1-, NHCOAr, NHCOR, NHCONHR; and wherein: X1- = 5-sulphosalicylate, tartrate, 10-camphosulphonate; A? = phenyl, 4-chlorophenyl, 2-furyl, 2-thienyl, 2-hydroxyphenyl, 2- acetoxyphenyl; The invention discloses the compound having the formula I wherein X, Y and Z are as defined in the specification with the proviso that when when X = O and Y = H, Z is other than H; and, when X = dimethylamino, diethylamino, pyrrolidino, piperidino or morpholino: Y = H; Z = H, CONHAr, CONHR; Ar = aryl, preferably phenyl; R = H, C1-C4 alkyl, C4-C6 cycloalkyl; with the proviso that when when Z = H, X is other than pyrrolidino. The invention is also for process preparation of compounds of the invention, novel intermediates and pharmaceutical composition comprising the compounds of the invention.

Full Text

NOVEL VERBENONE DERIVATIVES PROCESS FOR THEIR
PRODUCTION AND PHARMACEUTICAL COMPOSITION
COMPRISING THEM
(i) Verbenone derivatives
The present invention relates to novel derivatives of (1S,5S)-4,6,6-
trimethylbicyclo[3.1.1]hept-3-en-2-one), which is more commonly known as (-)-
verbenone.
The oxidation of a- and ß-pinene leads to complex mixtures of monoterpenoid
compounds, which are predominantly characterized by alcohol and carbonyl
functions; these mixtures have been used for some time, for example in Italy
(OZOPULMIN®), France (OZOTHINE®) and Germany (OZOTHIN®), in the
therapy of respiratory disorders, owing to their recognised balsamic,
bronchosecretolytic and antiseptic properties.
Among the above-mentioned monoterpene derivatives of the carbonyl type (-)-
verbenone, a ketone derivative having an a-pinane structure whose structural
formula is given below, is of particular interest.
(-)-verbenone is characterized by its particular pulmonary tropism, its anti-
inflammatory action on the respiratory tract and by its good muco-regulatory
capacity.
The anti-inflammatory activity on the respiratory apparatus has been investigated in a
series of classical experimental pharmacology tests: carrageenan- and dextran-
induced oedema, adjuvant arthritis (rat), passive cutaneous anaphylaxis (rabbit),
turpentine- and carrageenin-induced pleurisy (rat) and acrolein-induced aerosol
(guinea pig).
GB A-1585 831 and Masataka Watanabe et al. J. Org. Chem; vol. 58, no. 15 (1993) p.
3923-3927 both disclose the compound verbenone which is not in the ambit of the
compounds claimed by the present invention.
Y. Chretien-Bessiere et al., Comptes Rendus Hebdomadaires des Seances de
l'Academie des Sciences, Serie C: Sciences Chimiques, vol.264, no. 8 (1967), p. 710-713)
though discloses compounds wherein X in the compounds of the formulae I of the
present invention represents pyrrolidino and Y and Z represent H, this possibility has
been excluded from the scope of the present invention. Further, the document is silent
about the activity or use of the compounds disclosed therein.
The present invention solves the problem of oily nature, instability and water-
insolubility of (-)- verbenone by preparing derivatives in a solid, water-soluble form
while maintaining anti-inflammatory activity in respect of respiratory tract which is
typical of (-) verbenone. There is no hint in the prior art that (-) - verbenone derivatives
as disclosed in the present invention could solve the problem posed.
(-)-verbenone is an oily substance having a characteristic odour and a boiling point of
227-228° C, d= 0.975, no20 = 1.497 and a specific rotatory power which varies
according to the respective suppliers ( ACROS catalogue, purity 94%: -193°, c=10,
ethanol; FLUKA catalogue, purity = 99%: 180±2°, c=10, ethanol).
However, the oily nature, the instability over time and the water-insolubility of (-)-
verbenone make it difficult to administer in water-soluble dosage forms. Therefore,
the object of the present invention is to synthesize (-)-verbenone derivatives that are
in a solid, water-soluble form or which can in any case be readily converted into
water-soluble compounds, while maintaining and possibly increasing the anti-
inflammatory characteristics in respect of the respiratory tract which are typical of (-
)-verbenone.
Novel pharmacologically active derivatives of (-)-verbenone having the general
formula I given below:
wherein, when X = O
Z = H, =CHAr, =C(OH)COOEt, =NOR;
Y = H, NH2, NH3-X1-, NHCOAr. NHCOR, NHCONHR,
NHCONHAr;
X1- = pharmaceutically acceptable anion;
Ar = aryl or heteroaryl, preferably phenyl, 4-chlorophenyl, 2-furyl,
2-thienyl, 2-hydroxypbenyl, 2-acetoxyphenyl;
with the proviso that when X = 0 and Y = H, Z is other than H;
ylth the proviso that when Z = H, X is other than pyrrolidino
3.
R = H, C1-C4 alkyl, C4-C6 cycloalkyl, CH2COOH, CH2COOEt,
CH2COCH3, CH2CN, CH2COCH,COOEt, CH2C6H5;
and, when X = dimethylamino, diethylamino, pyrrolidino, piperidino or morpholino:
Y = H;
Z = H, CONHAr, CONHR;
Ar = aryl, preferably phenyl;
R=H, C1-C4 alkyl, C4-C6 cycloalkyl
have now been found, and form subject-matter of the present invention.
The broken line given in the general formula I is thus intended to indicate two
distinct structures resulting from the different positioning of the two conjugated
double bonds of (-)-verbenone, as is demonstrated more clearly by the general
formulae II and III given below:
wherein:
when Y = H, preferably Z = =CHAr, =C(OH)COOEt, =NOR (that is to say, the Z
siroup and the C-10 are joined by a double bond); or, alternatively,
when Z = H, preferably Y = NH2, NH3+X1-, NHCOAr, NHCOR, NHCONHR;
and wherein:
X1- = 5-sulphosalicylate, tartrate, 10-camphosulphonate;
A? = phenyl, 4-chlorophenyl, 2-furyl, 2-thienyl, 2-hydroxyphenyl, 2-
acetoxyphenyl;
The invention discloses the compound having the formula I
wherein X, Y and Z are as defined in the specification with the proviso that when when X
= O and Y = H, Z is other than H; and, when X = dimethylamino, diethylamino,
pyrrolidino, piperidino or morpholino:
Y = H;
Z = H, CONHAr, CONHR;
Ar = aryl, preferably phenyl;
R = H, C1-C4 alkyl, C4-C6 cycloalkyl;
with the proviso that when when Z = H, X is other than pyrrolidino.
The invention is also for process preparation of compounds of the invention, novel
intermediates and pharmaceutical composition comprising the compounds of the
invention.

Documents:

« Previous Patent

Next Patent »

Patent Number

225233

Indian Patent Application Number

IN/PCT/2001/01026/KOL

PG Journal Number

45/2008

Publication Date

07-Nov-2008

Grant Date

05-Nov-2008

Date of Filing

03-Oct-2001

Name of Patentee

EUPHAR GROUP SRL

Applicant Address

VIA SETTALA, 3, I-20124 MILANO

Inventors:

#	Inventor's Name	Inventor's Address
1	CORVI MORA PAOLO	VIA SCALABRINI, 49, I-29100, PIACENZA
2	RANISE ANGELO	VIA BORZONE, 21, INTERNO 13, I-16132 GENOVA

PCT International Classification Number

C07C 235/62

PCT International Application Number

PCT/EP00/03339

PCT International Filing date

2000-04-13

PCT Conventions:

#	PCT Application Number	Date of Convention	Priority Country
1	MI99A000799	1999-04-16	Italy