Title of Invention	METHOD OF ENRICHING Y-AMINOBUTYRIC ACID AND CEREAL OBTAINED BY THE METHOD .
Abstract	Water is slowly added to dry grain having a water content previously adjusted to 10% to 15% at a water addition rate of 0.5% to 2.0%/h, in orderr to provide a water content of 20% to 30%, and thereafter, the grain is placed into a tank, followed by tempering for 2 to 15 hours.

Full Text

METHOD FOR ENRICHING γ-AMINOBUTYRIC ACID AND CEREAL OBTAINED BY THE METHOD BACKGROUND OF THE INVENTION Field of the Invention The present invention relates to a method for enriching y-aminobutyric acid and grain obtained by the method. Description of the Related Art It has previously been known that a food material containing enriched γ-anmobutyric acid is obtained by immersing in water at least one member selected from the group consisting of rice germ, germ-containing rice bran, rice with germ, wheat germ, and wieat-germ-containing wheat gluten, under conditions consisting of a pH between 2.5 and 7.5 and a temperature of 50°C or lower (refer to Japanese Patent Application Laid-Open No. 7-213252, for example). According to the ai orementioned Japanese Patent Application Laid-Open Nc. 7-213252, it has been discovered that glutamic acid, which is a precursor substance of y-aminobutyric acid, is contained at a high concentration in the surface portions of germ, rice grain, and wheat, and that such glutamic acid is rapidly converted to y-aminobutyric acid when it is immersed in vater. However, in the method described in the aforementioned Japanese Patent Application Laid-Open No. 7-213252, since y-aminobutyric acid is specifically enriched only in the germ portion or bran layer, such y-aminobutyric acid is impaired during grain polishing. In addition, since germ or the like is immersed in water at 500C or lower for approximately 8 hours, the growth of microorganisms is activatedafter such immersion, and there is a risk of causing putrefaction. In order to solve such problems, it is possible to apply a method of immersing brown rice in water containing dissolved oxygen of 20 ppm or more to enrich γ-aminobut.yric acid (refer to Japanese Patent Application Laid-open No. 2000-300196, for example), or a method of intermittently adding hot water at 50°C or lower to brown rice in a dark room using a hot water shower, without immersion in water, in ordsr to adjust humidity and temperature, thereby preventing the undesirable odors caused by fermentation during germination (refer to Japanese Patent Application Laid-Open No. 2000-291423, for example). However, the method described in the aforementioned Japanese Patent Application Laid-open No. 2000-300196, which comprises immersing browr. rice in water containing dissolved oxygen of 20 ppm or more to enrich γ-aminobutyric acid, has been problematic in that immersion is carried out for 16 hours while exchanging water every 6 hours to increase the dissolved oxygen, thereby causing complicated operations. Moreover, in the method described in the aforementioned Japanese Patent Application Laid-Open No. 2 000-291423, which comprises intermittently adding hot weather at 50°C or lower to brown rice using a hot water shower, in order to produce germed brown rice, since the water absorption rate of rice grain has not been considered, there has been a risk of causing grain break. DISCLOSURE OF THE INVENTION Taking into consideration the aforementioned problems, it is a technical object, of the present invention to provide a method of adding water to grain to suppress denaturation of such grain, such as grain break, thereby significantly enriching y-aminobutyric acid contained therein, and to provide grain obtained by the above method. In order to achieve the aforementioned object, thepresent invention relates to a technical means for slowly adding water to dry grain, the water content of which has previously been adjusted between 10% and 15%, at a water addition rate of 0.5% to 2 .0%/h, in order to provide a water content of 2 0% to 3 0%; placing the grain into a r.ank; and tempering it for 2 to 10 hours. By this technical mecins, when water is added to grain, the water addition rate is limited to 0.5% to 2. 0%/h. Thus, the water absorption rate of grain becomes slow, and denaturation of the grain, such as grain break, is suppressed. At the same time, high physiological activity, utilization of the properties of germ with a high water absorption rate, and aerobic properties can be secured. Accordingly, as compared with the conventional method involving immersion in water, this technical means is able to significantly enrich γ-aminobutyric acid. In addition, when water is added to the above described dry grain, cloudy water droplets having a particle diameter of 0.1 mm or less are attached to the surface thereof so that slow addition of water is carried out, thereby allowing the water content to shift from the surface of the grain to its interior. Moreover, the above described hydrated grain may be tempered, while outside air may be introduced into the tank and ventilation may be provided. Furthermore, a gramineous (glasslike) plant such as rice, wheat, or corn, may be used as the above described dry grain. When brown rice is used as the above described, dry grain, water may be added to the brown rice having a water content previously adjusted to approximately 14%, at a rate of less than 0.5%/h, until the water content thereof reaches 17%, and after the water content thereof exceeds 17%, the water addition rate may be gradually increased, to a rate of 0.5% to 2.0%/h. As stated above, according to the present invention, water is slowly added to harvested and dried grain having a water content previously adjusted to 10% to 15% at a water addition rate of 0.5% to 2.0%/h, in order ~o provide a water content of 20% to 30%. Thereafter, the grain is placed into a tank, followed by tempering for 2 to 10 hours . Accordingly, when water is added to the grain, the water addition rate is limited to 0.5% to 2 . 0%/h, so that the water absorption rate of the grain becomes slow. Thus, while suppressing denaturation of the grain, such as grain break, γ-aminobuyric acid can be enriched in the albumen portion thereof. Thus, γ-aminobutyric acid enriched in the albumen portion is not damaged by grain polishing or the subsequent processing. Accordingly, even when the grain is processed into the form of rice with germ, polished rice, or rice flour, y-aminobutyric acid is maintained at a high concentration. Moreover, since the aerobic properties of grain are maintained in this methcd, y-aminobutyric acid can be significantly enriched, as compared with the conventional method involving immersion in water. In contrast, intheprior art technique, when y-aminobutyric acid is generated only in the germ portion or bran ] ayer of grain, such y-aminobutyric acid is almost completely ost by rice polishing or processing (refer to Table 1 below). Furthermore, when wa;er is added to the above described dry grain, cloudy water d::oplets having a particle diameter of 0.1 mm or less are attached to the surface thereof so that slow addition of water is carried out. Thus, fine water droplets that are much mo::e smaller than the water droplets provided by the conventional hot.water shower are attached to the surface of the grain, and thereafter, the water content is uniformly absorbed into the cell tissues of the grain. Accordingly, denaturation of the grain, such as grain break, can be suppressed. The hydrated grain obtained in that way is tempered, while outside air is introduced into the tank and ventilation is provided. Hence, an increase in he temperature in the tank is suppressed, in order to prevent degeneration of the grain, and at the same time, toxic gas generated in the tank can be eliminated. When brown rice is used as the above described dry grain, water is added to the brown rice having a water content previously adjusted to approximately 14%, at a rate of less than 0.5%/h, until the water content thereof reaches 17%. After the water content thereof exceeds 17%, the water addition rate is gradually increased to a rate of 0 .5% to 2 . 0%/h. Thus, the water absorption rate of the grain is suppressed until the water content of the brown rice reaches 17%, and denaturation of the grain, such as grain break, can be thereby prevented. When such brown rice water content exceeds 17%, the water absorption rate is increased, so that grain containing enriched γ-aminobutyric acid can be obtained in a shorter time than is usual. BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a process chart showing one embodiment of a method of enriching GABA FIG. 2 is a view shoeing the relationship between the water content of brown r:.ce and a water addition rate; FIG. 3 is a schematic front view of a device for carrying out the method of enrich;.ng GABA; FIG. 4 is a schematic s Lde view of a water-spraying device; FIG. 5 is a view showir.g a comparison between normal white rice and the functional white rice of the present invention in terms of GABA content inalbumen in the case of a polished rice yield of 90%; FIG. 6 is a view showirg a comparison between normal white rice and the functional white rice of the present invention in terms of magnesium content in albumen in the case of a polished rice yield of 90%; Figure 7 is a view showing a comparison between commercially available wheat flour F and the wheat flour of the present invention in terms of GABA content; and Figure 8 is a view shoeing a comparison between the case of subjecting corn grains to an immersion treatment and the case of subjecting corn grains to a spraying treatment, in terms of the amount of GABA generated. DETAILED DESCRIPTION of THE PREFERRED EMBODIMENTS FIG. 1 is a process chart showing one embodiment of a method of enriching γ-aminobutyric acid (hereinafter referred to as "GABA"). FIG. 2 is a view showing the relationship between the water content of brown rice and a water addition rate. Hereafter, the method of enriching GABA of the present invention will be described with reference to FIGS. 1 and 2. With regard to grain used as a raw material, gramineous (glasslike) plants such as rice, wheat or corn, or cereals containing a large amount of protein, such as soybeans, can be used. When brown rice is used a such grain, for example, harvested and dried grain having a water content previously adjusted to approximately 14% is used (step 1 in FIG.l). Subsequently, water is slowly added to such a raw material, resulting in a water contert between 20% and 30% (step 2 in FIG.l). In order not to cause denaturation of grain, such as grain break, with regard to such a treatnent to slowly add water to grain, water is added to the gredn at a rate of increasing the water content of the grain to 0. 5% per hour (0.5%/h), until the water content thereof reaches .'.7% . After the water content thereof exceeds 17%, the water addition rate is gradually increased, and water is added at a rate between 0 . 5% and 2 . 0%/h (see FIG. 2) . It is desired that a preferred water addition method comprise spraying water to form cloudy water droplets and attaching the water droplets on the surface of brown rice. The diameter of such a cloudy water droplet is preferably set at 0.1 mm or less. After completion of such water addition, the grain is transferred into a tank or the like, and tempering (which means an operation to leave grain for a certain period of time in order to achieve the equ.-iibration of the water added to the grain) is then carried out for 2 to lO hours (step 3 in FIG. 1) . The optimum envirormental temperature is between 16°C and 24°C during such tempering. If outside air is introduced into the tank and ventilation is provided, the environment becomes more preferable. The temperature of water is the same as the temperature of rvnning water, and it is unnecessary to adjust the pH thereo::. A series of operations can be sufficiently carried out at a room, temperature of approximately 20°C. After completion cf the tempering, the grain is dried by a conventional drying nethod, unti1 it has a. water content suitable for rice polishincr (step 4 in FIG. 1) . Thereafter, it is possible to apply a rice polishing method (step 5 in FIG. 1) or a method of pclishing the rice while maintaining a high water content of the grain after completion of the tempering (step 6 in Fig. 1) . Otherwise, it may also be possible that the grain after completion of the tempering be heated as appropriate, without grain polishing, in order to process it in the form of parboiled rice or pregelatinized rice. In the case of the grain obtained in that way, a GABA content and a magnesium content in the albumen thereof at a "polished rice yield of 90% are significantly higher than those of normal white rice. Subsequently, an example of a device for carrying out the method of enriching CABA will be described with reference to FIGS. 3 and 4. FIG. 3 is a schematic front view showing a device for carrying out the method of enriching GABA. FIG. 4 is a schematic side visw showing a water-spraying device. In FIG. 3, reference numeral 1 denotes a water-spraying device. The water-spraying device 1 comprises, as main components, a grain elevator 3 provided in a machine casing 2, dual storage tanks 4,5 for storing the grain elevated by the grain elevator 3, a belt conveyor 6 for transporting the grain discharged from the storage tanks 4,5 in the form of a layer, and a rotary ba.:rel 7 for adding water to the grain transported by the belt conveyor 6. A raw material-supplying hopper 8 for supplying grain as a rawmaterial is provided at the bottom of the grain elevator 3, and a falling gutter 9 equipped with a bidirectional valve is provided at the top of the grain elevator 3 . In the above falling gutter 9, a supply pipe 10 directed towards the storage tank 4 and a supply pipe 11 directed towards the storage tank 5 are provided. At the bottom of the storage tanks 4,5, an opening and closing shutters 12,13 are provided, whereby the grain is fallen at an appropriate flow rate. The belt conveyor 5 is formed by winding an endless conveyor belt 6c around a ;?air of sprockets 6a, 6b. Asupplying gutter 14 for supplying grain into the rotary barrel 7 is providedat the transportation terminating portion of the belt conveyor 6 . In the suppl /ing gutter 14 , a water content sensor 42 for surveying the water content of grain before being supplied into the rotary barrel 7 is provided. The type of this water content sensor 42 is not particularly limited, as long as it is able to precisely measure the water content of grain. Examples of such a water content sensor may include an electric resistance type, a capacitance type, and a near infrared radiation type The surrounding portion of the above rotary barrel 7 is supported by a plurality of motor rollers 15 in a rotatable manner. In addition, in the rotary barrel 7, the grain supplying side is disposed at a higher position, and the grain discharging side is disposed at a lower position. On the grain discharging side of the rotary barrel 7, there is provided a discharging gutter 17 equipped with a switching valve 16 for discharging the grain in order to use it to the next step or for circulating the grain to the water-spraying device 1. Referring to FIG. 4, a plurality of impellers 18 are provided on the internal surface of the above rotary barrel 7 along the direction of the length thereof. When grain is supplied into the rotary barrel 7, the grain is lifted up by the impellers 18 as a result of the rotation of the barrel 7, and it is then fallen imnediately before reaching the upper portion, so that the grain can be stirred. An atomizer 19 is provided in the barrel 7 . As such an atomizer 19, a two-phase flow nozzle for atomizing liquid utilizing a high-speed flow such as compressed air, or a device for atomizing a liquid into the form of particles with a diameter of 0.1 mm or less by ultrasonic wave oscillation and then suspending the particles in the air current in the form of fog using a fan, can also be adopted. In the present embodiment, a two-phase flow nozzle for generating- fine fog with a mean particle diameter between 10 and 50 µm was used as the atomizer 19 (for example, model BIMV02, manufactured by Ikeuchi Company). In FIG. 3 , reference numeral 2 0 denotes a tempering tank. This tempering tank comprises a tank portion 21, a supply valve 22 provided at the upper portion of the tank portion 21, a supply hopper 23 that is collected with the supply valve 22, a discharge valve 24 provided at the bottom portion of the tank portion 21, a discharge- gutter 25 that is connected with the discharge valve 24, and a ventilator 26 for taking fresh air such as outside air into the tank portion 21. An air incorporating port 27 and an air discharging port 28 are provided on the tank portion 21. The above ports are connected with the above ventilato:: 26 via circulation pathways 29, 30, respectively. The ventilator 26 has a simple structure, which comprises a dust prevent..ve filter 31 for eliminating dusts from outside air and a fan 32 for aspirating fresh outside air and supplying it to :he tank portion 21. For example, ventilation can be carried out in the tank portion 21 at an air quantity of 6 m3/ton•1. Reference numeral 33 denotes a transportation means for connecting the water-spraying device 1 in the previous step w:.th the tempering tank 20. Moreover, in FIG. 3, reference numeral 34 denotes a grain polisher. This grain polisher comprises a polishing trochanter 35, which is a:tached to a main axis (not shown in the figure) and has a polishing roll, and a bran-removing polishing tube 3 6 having a porous wall, which is provided around the above polishing trochanter 35. A polishing chamber 37 is provided in a space between the polishing trochanter 35 and the bran-removing polishing tube 36. Also, a discharge port (not shown in the figu:re) is provided at the end portion of the polishing chamber 37 . Moreover, an external resistance 38 comprising a resistance board for pressing towards the above discharge port and an anchor is provided. Reference numeral 39 denotes a supply hopper o:: the grain polisher 34, reference numeral 40 denotes a polished product-discharging gutter of the grain polisher 34, and reference numeral 41 denotes a transportation means for connecting the tempering tank 2 0 in the previous step with the grain polisher 34. Hereafter, the actions of the devices shown in FIGS. 3 and 4 will be described. Grain used as a raw material having a water content previously adjusted to approximately 14%, is supplied from the raw material-supplying hopper 8. The grain is elevated by the grain elevator 3, and it is then stored in the storage tanks 4,5. Thereafter, the grain discharged from the storage tanks 4, 5 is transported by the belt conveyor 6 in the form of a layer, and it is then supplied into the rotary barrel 7 via the supplying gutter 14 . The rotary barrel 7 rotates at a rate of 6 to 12 revolutions /minute in order to stir the grain, and at the same time, water is added by the atomizer 19 in the from of fine fog with a mean particle diameter between 10 and 5 3 µm. During this step, water is added, while the water content of the grain is surveyed by the water content sensor 42 provided in the supplying gutter 14. That is to say, water is added, while the grain is circulated via the grain elevator 3. Thus, water is added to the grain at a water addition rate of 0. 5%/h until the water content thereof reaches 17% . After the water content thereof exceeds 17%, the water addition rate is gradually increased to a rate of 0.5% to 2.0%/h When the water content reaches 20% to 30%, circulation of the grain is terminated, in order to terminate the water addition, and the grain is discharged from the discharging gutter 17. Thereafter, the grain having a water content between 2 0% and 3 0% is supplied to the sapply hopper 23 of the tempering tank 20 by the transportation means 33. The grain supplied to the supply hopper 23 is accumulated and stored in the tank portion 21 by opening the supply valve 22. The grain accumulated and stored in. this way is then subjected to tempering for 2 to 15 hcurs. The optimum environmental temperature applied durir.g such tempering is between 16°C and 24°C. In order to incorpoi ate outside air into the tankportion 21, the ventilator 2 6 may be operated, in order to carry out ventilation. That is, when the fan 32 is operated, outside air, from which dusts have been removed by the dust preventive filter 31, is incorporated, and the outside air is then supplied to the tank portion 21 via the circulation pathway 29. The air is then discharged from the tank portion 21 via the circulat ion pathway 30 . Herein, the air quantity is 6m3/ton-h or less, for example. After completion of the tempering, the grain is discharged from that tempering tank 20 by the discharge valve 24 through the discharge gutter 25. The grain tempered in the tempering tank 20 is supplied to the supply hopper 39 of the grain polisher 34 by the transportation means 41, followed by grain polishing. That is to say, the embodiment shown in FIG. 3 indicates a method for directly polishing the grain with a high water content (a water content between 2 0% and 30%) obtained after completion of the tempering (refer to step 6 in FiG.'l). However, the grain polishing method is not limited thereto, and a commercially available gra:.n-drying machine may be provided as a pre-step of the grain polisher 34. The tempered grain with a high water content is supplied to the polishing chamber 37 of the grain polisher 34, and it is then polished by the polishing trochanter 35. In addition, since the discharge port of the polishing chamber 37 is compressed by the external resistance 38, the inside of the polishing chamber 37 becomes a moderately high-pressure state, so that a bran layer or germ can be peeled, thereby conducting grain polishing. In the case of the c-rain obtained in that way, when a white rice yield is 90%, for example, a GABA content and a magnesium content in albunen are significantly increased, as compared with normal whi :e rice. Example 1 Brown rice was used as ,i rawmaterial, and the water content thereof had previously been adjusted to approximately 14%. Thereafter, slow water addition was carried out, in order to provide a water content of 20% to 30%. In order not to cause rice break, such a slow wa~:er addition treatment was carried out at a rate of 0.5%/h, until the water content reached 17%. After the water content exceeded 17%, the water addition rate was gradually increased to a rate of 0.5% to 0.9%/h. As such water to be added, drinkable water such as running water, distilled water, well water, acidic water,, or electrolytic saline water, is preferabLe. In particular, when sterilization is also intended, a hypochlorite solution having an effective chlorine concentration between 0.1 ppm and 50 ppm may also be used. A preferred water addition method comprises spraying water, in order to add cloudy water droplets onto the surface of brown rice. The diameter of such an atomized water droplet was set at 0.1 mm or less. After completion of such water addition, the brown rice was transferred into a tank or the like, followed by tempering for 2 to 10 hours. The optimum environmental temperature during such tempering was ;;et between 16°C and 24°C, and outside air was introduced into the tank for ventilation. As a water temperature, the temperature of running water was applied. The control of a pH thereof was not necessary. A series of operations were carried out at a room temperature of approximately 20°C. After completion of the tempering, the brown rice or husk was dried by a conventional drying method, until the water content thereof was adjusted to a degree suitable for hulling and polishing. Thereafter, the brown rice or husk was subjected to hulling and polishing. In the case of the polished rice obtained in that way, when a yield was 90%, the GABA content in the albumen thereof was approximately 20 times increased as comparedwith that of normal white rice (see FIG. 5) . The magnesium content in the albumen thereof was approximately 3 times increased, as compared with that of normal white rice, when a yield was 90% (see PIG. 6). Example 2 Brown rice ("Hoshinoyume" or "Kirara") was used as a raw material, and the water cor. tent thereof had previously been adjusted to approximately 14%. Thereafter, slow water addition was carried out, ir. order to provide a water content of 20% to 3 0%. In order not to cause rice break, such a slow water addition treatment was carried out at a rate of 0.5%/h, until the water content reached 17%. After the water content exceeded 17%, the water addition rate was gradually increased to a rate of 0.5% to 0.9%/h. As such water to be added, running water or distilled water is preferably used. When sterilization is also intended, ahypochlorite solution having an effective chlorine concentration between 0.1 ppm and 50 ppm may also be used. A preferred water addition method comprises spraying water, ;.n order to add cloudy water droplets onto the surface of brown rice. The diameter of such an atomized water droplet wc.s set at 0.1 mm or less. After completion of such water addition, the brown rice was transferred into a tank or. the like, followed by tempering for 2 or 3 hours . The optimum, environmental temperature during such tempering was set between 16C'C and 24°C, and outside air was introduced into the tank for ventilation. As a water temperature, the temperature of running water was applied. The control of a pH thereof was not necessary. A series of operations were carried out at a room temperature of approximately 20°C. After completion of the tempering, the brown rice or husk was subjected to evaporation by heating at 130°C for 3 minutes. It was then dried by a conventional drying method, until the water content thereof was adjusted to a degree suitable for hulling and polishing. Thereafter, the brown rice or husk was subjected to hulling and polishing. In the case of the pol-.shed rice obtained in that way, when a yield was 90%, the GABA content thereof was 4.3 times in the albumen portion thereof, the GABA content in the germ potion thereof was 4.4 times, and the GABA content in the bran layer portin thereof was 1.2 times increased, as compared with the case where no enrichment treatment was carried out (refer to Table 1) . In terms of the disintegration rate of rice grain obtained in the. case where the obtained brown rice was immersed in water for 24 hours, the present production method was compared with ;he conventional production method of immersing grain in water for enrichment. As a result, it was found that the disintegration rate of the conventional production method was 79% whereas that of the present production method was 10%. Accordingly, using the present production method, the form of rice can be maintained even during rice boiling, and as a result, brown rice that is excellent in terms of appearance and taste, having only a slight extent of elution of starch, can be obtained. Example 3 Wheat grain was used ,as a raw material, and the water content thereof had previously been adjusted to approximately 12%. Thereafter, slow watei addition was carried out, inorder to provide a water content of 21% to 25%. In order not to cause denaturation of the wheat grain, such a slow water addition treatment was carried out by the aforementioned spray method at a water addition rate of 1.0% to 2.0%/h, until the water content reached 21% to 3 0%. As such water to be added, running water was used. After completion of such water addition, the wheat grain. was transferred into a tank or the like, followed by tempering for 3 to 10 hours. The optimum environmental temperature during such tempering was set between 7°C and 13°C, and outside air was introduced into the tank for ventilation. After completion of the tempering, the wheat grain was dried by a conventional drying method, until the water content thereof was adjusted to a degree suitable for milling. Thereafter, che wheat grain was subjected to milling. When the wheat flour obtained in that way was compared with commercially availabl.e wheat flour F, the GABA content was approximately 3.8 timss increased (see FIG. 7). Example 4 Corn grain was used as a rawmaterial, and the water content thereof had previously been adjusted to approximately 14%. Thereafter, slow water addition was carried out, in order to provide a water content of 1.5% to 30%. In order not to cause denaturation of the corn girain, such a slow water addition treatment was carried out by the aforementioned spray method at a water addition rate oif 1.2% to 2.0%/h. As such water to be added, running water was used. After completion of such water addition, the corn grain was transferred into a tank or the like, followed by tempering for 5 to 15 hours. The optimum environmental temperature during such tempering was set between 10°C and 3 0°C, and outside air was introduced into the tank for ventilation. After completion of the tempering, the corn grain was dried by a conventional drying method, until the water content thereof was adjusted to a degree suitable for hulling, germremovaL, milling, and grinding. Thereafter, the corn grain was subjected to hulling, germ removal, milling, and grinding. When the corn grain obtained in that way was compared with corn grain obtained by continuous immersion, the GABA content was approximately 1.9 to 2.3 times increased (see FIG. 8) . The scope of the .nvention does not extend to the use of grain obtained by the method of the invention as seed. We Claim: 1. A method for enriching y-aminobutyric acid contained in grain such as herein described, comprising the steps of : (a) ^ drying harvested grain until its water content reaches 10% to 15% in order that said grain with water content of 10* to 15% is used. as starting material; (b) slowly adding water to dry grain of step (a) having a water content of 10% ::c 15%, at a water addition rate of 0.5% to 2.0%/h, in order to provide a water content of 20% to 30%; and (c) placing t.he grain int.) a tank and tempering it for 2 to 15 hours. 2. The method for enreachng y-amincbutyric acid as claimed in claim 1, where n when water is added to said dry grain, cloudy wafer droplets having a particle diameter of 0.1 mm or less is attached to the surface thereof so that slow addit on of water becomes possible. 3. The method for enriching y-aminobutyric acid as claimed In claim 1 or ,', vierein said hydrated grain is tempered, while outside air is introduced into the tank and ventilation Is provide. 4. The method for enriching y-aminobutyric acid as claimed in any one of cI airs ] to 3, wherein a gramineous (glasslike) plant such as rice, wheat, or corn, is used as said dry grain. 5. The method for emiching y-aminobutyric acid as claimed in any one of claims 1 to 4, wherein when brown rice is used as said dry grain, water is added to the brown rice having a water content previously adjusted to approximately 14%, at a late of 0.5%/h, until the water csontent thereof reaches 1'%, and after the water content thereof exceeds 17%, the vater addition rate is gradually increased to a rate of 0.5% to 2.0%/h. 6. Grain such as herein described containing enriched y-aminobutyric acid, which is obtained by enriching y- aminobutyric acid contained in the grain by the method as claimed in any one of claims 1 to 5, and then polishing the grain. 7. Grain as claimed in claim 6 which contains 8 mg/100 g or more of y-aminobutyr LC acid in the albumen portion thereof. Water is slowly added to dry grain having a water content previously adjusted to 10% to 15% at a water addition rate of 0.5% to 2.0%/h, in orderr to provide a water content of 20% to 30%, and thereafter, the grain is placed into a tank, followed by tempering for 2 to 15 hours.

Documents:

261-KOLNP-2006-FORM-27.pdf

261-kolnp-2006-granted-abstract.pdf

261-kolnp-2006-granted-assignment.pdf

261-kolnp-2006-granted-claims.pdf

261-kolnp-2006-granted-correspondence.pdf

261-kolnp-2006-granted-description (complete).pdf

261-kolnp-2006-granted-drawings.pdf

261-kolnp-2006-granted-examination report.pdf

261-kolnp-2006-granted-form 1.pdf

261-kolnp-2006-granted-form 18.pdf

261-kolnp-2006-granted-form 3.pdf

261-kolnp-2006-granted-form 5.pdf

261-kolnp-2006-granted-pa.pdf

261-kolnp-2006-granted-reply to examination report.pdf

261-kolnp-2006-granted-specification.pdf