Title of Invention	DEVICE FOR SEPARATING A MIXTURE THAT COMPRISES AT LEAST ONE GASEOUS PHASE AND A LIQUID PHASE
Abstract	Device that is integrated ina chamber that allows separation of a mixture that comprises at least one gaseous fraction and one liquid fraction, whereby said mi8xture is obtained a zone for bringing into contact a liquid phase, a gaseous phase and solid particlles.

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The plant Phyllanthus amarus which is found growing all over the plains of India, is best known for its use in jaundice and more recently for the treatment of Hepatitis-B. However, in traditional medicine the leaf poultice has been used in itch and other skin affections and whole plant including the root is combined with rice water and used as a poultice in ulcers, sores and swellings. Various extracts of Phvllanthus amarus have been suggested for the treatment of other viral infections like Hepatitis-B and AIDS. However as far as we are aware Phvllanthus amarus has not been suggested for the treatment of herpes simplex viral infections. There is a steadily increasing incidence of opportunistic infections caused by several viruses especially herpes simplex virus (HSV), varizella-zoster virus (VZV), cytomegalovirus (CMV) etc. in immunodeficient patients which has triggered of a search for new treatment modalities including those derived from plants. This invention describes a process to obtain an active preparation from aerial parts of Phvllanthus amarus which shows activity against herpes simplex virus and which can be used in the treatment of infection due to herpes simplex virus. In the present invention, it has been possible to obtain an extract consisting mostly of tannins and lignin’s which has activity against herpes simplex virus shown by cenotaphic effect inhibition (CPE). Thus, extraction of aerial parts of Phvllanthus amarus using 70% acetone in water (v/v) gave material which showed CPE inhibition against HSV at 2 mg/ml. inks material was then analysed by HPLC and had a profile shown in Fig.1. The peaks obtained were identified by comparison with the peaks obtained from isolated compounds. The structures of the compounds were determined by comparison of spectroscopic data with literature Information. Thus, the major peaks in the HPLC are corilagin, geraniin, phyllanthin, hypophyllanthin and niranthin when viewed at 220 nm and in addition rooting and ellagic acid when viewed at 254 nm. The main advantages of this invention are : 1) Simple process for the preparation of the active extract. 2) Identification of the major components of the active extract. 3) Identification of the relative amounts of the various components present, thus standardizing the extract composition. Thus, in a typical experiment powdered aerial parts of Phyllanthus amarus were extracted at ambient temperature with acetone: water containing 70% of acetone. After keeping for 24-36 hours with the solvent mixture, the solvent was removed and the remaining aqueous portion was evaporated to dryness under reduced pressure. The process is further illustrated by the following example which is provided by illustration and therefore not to be construed to limit the scope of invention. Example Preparation of extract Aerial parts of Phyllanthus amarus were dried and powdered. 500g of the powdered material was extracted with 2x3500 ml of acetone: water (7:3) at ambient temperature for 2x24 hours. The combined extract was filtered and evaporated to dryness unclear reduced pressure. HPLC Studies A typical HPLC profile of the extract Is shown in Fig.1. HPLC Studies The HPLC was carried out with a LC-10AD (Shimadzu) with Escrow liquid chromatograph and a SPD-M10A photodiode array detector on Lithospheres 100 RP-18 (5μm) column (250x4mm) at two different detection wavelengths of 220 nm and 254 nm respectively. The mobile phase used for the separation was acetonitrile (Solvent-A) and water (Solvent-B) each containing 0.05% H3PO4, at a flow rate of 1 ml/min, using a gradient from 5% A to 90% A in 60 minutes. The injected volume of the sample was 10μ1. A typical HPLC profile of the extract Is shown in Fig.1. We Claim: 1. A process for the preparation of an active extract from the plant Phyllanthus Amarus for the treatment o infection caused by the herpes simplex virus comprising the steps of carrying out at least on extraction of the powdered aerial parts of the sail plant with a solvent comprising acetone-water mixture in the proportion range 1:5 to 1:10 weight/volume; anc removing, by known means, the solvent thereafter. 2. A process as claimed in Claim 1 wherein the acetone-water mixture contains 60%, to 80 % acetone by volume of the mixture. 3. A process for the preparation of an active extract from the plant Phyllanthus Amarus for the treatment of infection caused by the herpes simplex virus, substantially as herein described and as i1lustrated. . 4. Active extract prepared from the plant Phyl1anthus Amarus for the treatment of infection caused by the herpes simplex virus whenever prepared by a process as claimed in any one of the preceding Claims. Dated this 21st day of May 1999

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0503-mas-2002 abstract.pdf

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