Title of Invention	"METHOD FOR TREATING SEEDS, AND SEEDS OBTAINABLE WITH THE METHOD"
Abstract	A method for treating seeds, comprising the following steps: (a) a sterilization step wherein the seeds have an initial temperature of -30°C to 30° C, that they are contacted with steam for 0.1 to 5 seconds, and at a minimum pressure of 0.5 bar absolute, the seeds are contacted with a medium having a condensation temperature of 80-200°C, wherein the medium condenses on the seeds and the temperature of the surface of the seed is raised to 200 °C at most; and (b) a cooling step wherein the sterilized seeds obtained in step (a) cooled, wherein the temperature of the surface is lowered within 10 seconds to 30° C at most, and wherein the conditions of steps (a) and (b) are set such that the germination capacity of the seeds is relatively preserved or increases.

Title of Invention

"METHOD FOR TREATING SEEDS, AND SEEDS OBTAINABLE WITH THE METHOD"

Abstract

A method for treating seeds, comprising the following steps: (a) a sterilization step wherein the seeds have an initial temperature of -30°C to 30° C, that they are contacted with steam for 0.1 to 5 seconds, and at a minimum pressure of 0.5 bar absolute, the seeds are contacted with a medium having a condensation temperature of 80-200°C, wherein the medium condenses on the seeds and the temperature of the surface of the seed is raised to 200 °C at most; and (b) a cooling step wherein the sterilized seeds obtained in step (a) cooled, wherein the temperature of the surface is lowered within 10 seconds to 30° C at most, and wherein the conditions of steps (a) and (b) are set such that the germination capacity of the seeds is relatively preserved or increases.

Full Text	Title: Method for treating seeds, and seeds obtainable with the method The invention relates to a method for treating seeds, and seeds obtainable with the method- In general, seeds are subjected to a sterilization treatment in order to kill the pathogens present on the seeds so that the transmission of pathogens to the crop to grow from the seeds is avoided and harm to the seeds themselves ia prevented. Such a treatment also increases the storage life of seeds. This applies to both seeds for sowing and seeds which are, possibly after germination, suitable for consumption or industrial processing. For seeds intended for reproduction or for consumption or industrial processing after the start of the germination process, it is logically of vital importance that the germination capacity, or the enzyme activity, is preserved. Seeds suitable for direct consumption are usually treated under such conditions that all germination capacity, if present at all, is completely lost. In this context, reference can be made to, for instance, international patent application WO 96/20606, in which seeds, herbs and spices are sterilized by means of a heat treatment which may, for instance, be carried out with steam. While the taste and smell of the seeds which are subjected to such a treatment are well preserved, the germination capacity of seeds which are subjected to such a treatment is completely lost. Seeds whose germination capacity needs to be preserved, by contrast, are nowadays treated with chemicals by, means of which the number of pathogens on the surface of the seeds is reduced, while the germination capacity can indeed be preserved. However, particularly for biological cultivation, and for seeds which are consumed or processed when germinated, the use of such chemical a is highly undesired, while the norms regarding chemical additions for traditional seed production are becoming increasingly stringent. The object of the present invention is therefore to provide a method for treating seeds, in which the seeds are sterilized without chemicals needing to be used, and in which at least a considerable extent of the germination capacity of the seeds is preserved or even increases. It has now surprisingly been found that this can he achieved by a combination of a specific sterilization step and a specific cooling step. The invention thus relates to a method for treating seeds, comprising the following steps: (a) a sterilization step in which, for 0,1 to 60 seconds and at a minimum pressure of 0.5 bar absolute, the seeds are contacted with a medium having a condensation temperature of 80-200°C, wherein the medium condenses on the seeds and the temperature of the surface of the seeds is raised to 200°C at most; and (b) a cooling step in which the sterilized seeds obtained in step (a) are cooled, wherein the temperature of the surface of the seeds is lowered, within 30 seconds at most, to 50°C at most, and the conditions of steps (a) and (b) are set such that the germination capacity of the seeds is relatively preserved or increases. The seeds obtained with the method according to the invention exhibit a long storage life, while the germination capacity is at least to a considerable extent preserved or even increases. Very surprisingly, it has been observed that the seeds treated with the method according'to the invention often cave a better germination capacity than untreated seeds- In the context of the present invention & "relative preservation of germination capacity" is understood to mean that the germination capacity as such is usually preserved, but that, in certain cases, the germination capacity can decrease by 25% at most, that is, a minimum preservation of 75% of the germination capacity such as it is present before the treatment. In the use of the present invention, the condensing medium condenses on. the surface of cold seed in step (a), the surface being very rapidly and uniformly heated, after which, before the heat can penetrate the seed, in step (b), the formed condensate rapidly evaporates so that the surface of the seed is cooled. The condensing medium may, for instance, consist of steam, but other media having a condensation temperature of 80-200°C are also suitable. A skilled person will understand that the parameters to be selected for step (a), such as time, pressure and temperature, depend on the size of the seeds, the type of seed and the properties of the condensing medium. For instance, a certain type of seed having larger dimensions may usually be exposed to a steam treatment longer, while preserving germination capacity, than a type of seed having smaller dimensions. Step (a) is carried out for 0.1-60 seconds, preferably for 0.1-5 seconds, and still more preferably for 0.5-1.5 seconds. In step (a), the surface of the seeds will teach a temperature of 200°C at most, before the seed parts are subjected to step (b). Preferably, in step (a), the surface of the seeds reaches a temperature of 80-150°C. Step (a) is preferably carried out at a pressure of 0,5-20 bar absolute, still more preferably a pressure of 1-6 bar absolute. Preferably, in step (b), the seeds are cooled by means of a vacuum treatment or another manner in which rapid evaporation occurs, Step (b) is carried out such that, within 30 seconds, the temperature of the surface of the seeds is lowered to 50°C at most. Preferably, step (b) is carried out such that, within 10 seconds, the temperature of the surface of the seeds is lowered to 30°C at most. Preferably, step (b) is carried out for 0.5-30 seconds, more preferably for 0.5-5 seconds, and still more preferably for 0.5-1 seconds. In step (a), preferably, steam is used as the condensing medium. The condensing medium may optionally be superheated. The initial temperature of the seeds is preferably -30-30°C, more preferably 0-80°C and still more preferably 1O-26qC. For the sake of completeness, it is noted that all above-mentioned limit ranges comprise both the limit values mentioned above and below. It will be clear to a skilled person that the initial temperature of the seeds is understood to mean the temperature of the seeds just before or at the moment when they are subjected to the sterilization step. Preferably, the method according to the invention is carried out such that the temperature of the germinating part of the seeds does not greatly exceed the initial temperature of the seeds. Preferably, the initial temperature of the germinating part of the seeds is essentially preserved. Although a small increase of the initial temperature will not directly have a great adverse influence on the germination capacity of the seeds, and sometimes even a positive influence, it will be clear that the germination capacity of the seeds will increasingly be harmed according as the temperature of the germinating part of the seeds more greatly exceeds the initial temperature during the treatment. The harm to the germination capacity depends on the maximum temperatures reached by the germinating parts of the seeds and the time during which these temperatures occur, This will differ per type of seed. By means of the method according to the present invention, also, seeds can be treated which are suitable for consumption or industrial processing, The seeds to be treated with the method, according to the invention are those plant parts which can be used for reproduction and can be chosen from the group of seed kernels, bulbs, tubers, cuttings and rhizomes, but are not limited to this group. The invention further relates to seeds which can be obtained by means of the method according to the invention. Example 1 In experiments 1-10, barley seeds were treated with the method according to the invention. The duration and pressure in steps (a) and (b) were varied, and these are shown in Table 1. In experiments 1-5, the initial temperature of the cores of the seeds was 20°C, while in experiments 6-10 the initial temperature of the cores of the seeds was -20°C. In all experiments, the minimum supply temperature of the steam waa 120°C, and froth experiment 5, the steam was superheated to 160°C, It was then determined to what extent the treatment according to the invention had influenced the number of microorganisms on the seed, and what the effect on the germination of the seed was. As a control, also, the contents of microorganisms and the germination were determined of barley seeds which were Not subjected to the treatment according to the invention, and whose initial temperature was 20°C and -20°C, respectively. Table1 (Table Removed) After each treatment, the seeds (8g, approximately 60 seeds) were shaken for 3 hours with 10 ml of sterile demineralized water. Subsequently, 100 µl of a 1:1000 dilution of the water was spread on an LB plate and, for two days, incubation of the LB plates took place in an incubator at a temperature of 37°C. After this, the seeds obtained with the treatments were placed to germinate in Petri dishes between 2x2 layers of filter paper (with 3 and 2.5 ml of water, respectively). Incubation in the dark took place at 20°C for one week. Figs. 1 and 2 show the results of the number of colonies that could be counted on the Petri dishes and the maximum number of seeds that germinated after each treatment. Fig. 1 dearly shows that the number of microorganisms on the seeds decreased when the seeds were treated with the method according to the invention, while Fig. 2 clearly shows that the treatment according to the invention had a positive influence on the germination capacity of the seeds when the treatment took place for a relatively short time. WE CLAIM: 1. A method for treating seeds, comprising the following steps: (a) a sterilization step wherein the seeds have an initial temperature of -30°C to 30° C, that they are contacted with steam for 0.1 to 5 seconds, and at a minimum pressure of 0.5 bar absolute, the seeds are contacted with a medium having a condensation temperature of 80-200°C , wherein the medium condenses on the seeds and the temperature of the surface of the seed is raised to 200 ° C at most; and (b) a cooling step wherein the sterilized seeds obtained in step (a) cooled, wherein the temperature of the surface is lowered within 10 seconds to 30° C at most, and wherein the conditions of steps (a) and (b) are set such that the germination capacity of the seeds is relatively preserved or increases. 2. A method as claimed in claim 1, wherein the step (a) is carried out for 0.1-5 seconds. 3. A method as claimed in claim 2, wherein step (a) is carried out for 0.5-1.5 seconds. 4. A method as claimed any one of claims 1 to 3, wherein, is step (a), the surface of the seeds reaches a temperature of 80-150° C, before the seeds are subjected to step (b). 5. A method as claimed in any one of claims 1 to 4, wherein the step (a) is carried out at a pressure of 0.5-20 bar absolute. 6. A method as claimed in any one of claims 1 to 5, wherein in step (b) the seeds are cooled by means of a vacuum treatment or another manner in which rapid evaporation occurs. 7.. A method as claimed in any one of claims 1 to 6, wherein step (b) is carried out such that, within 10 seconds, the temperature of the surface of the seeds is lowered to 30 ° C at most. 8. A method as claimed n claim 7, wherein step (b) is carried out for 0.5-5 seconds. 9. A method as claimed in any one of claims 1 to 8, wherein, in step (a), steam is used as the condensing medium. 10. A method as claimed in any one of claims 1 to 9 wherein the seeds have an initial temperature of 0-30° C. 11. A method as claimed in any one of claims 1 to 10, wherein the seeds have been chosen from the group of seed kernels, bulbs, tubers, cuttings, and rhizomes.

Full Text

Title: Method for treating seeds, and seeds obtainable with the method
The invention relates to a method for treating seeds, and seeds obtainable with the method-
In general, seeds are subjected to a sterilization treatment in order to kill the pathogens present on the seeds so that the transmission of pathogens to the crop to grow from the seeds is avoided and harm to the seeds themselves ia prevented. Such a treatment also increases the storage life of seeds. This applies to both seeds for sowing and seeds which are, possibly after germination, suitable for consumption or industrial processing. For seeds intended for reproduction or for consumption or industrial processing after the start of the germination process, it is logically of vital importance that the germination capacity, or the enzyme activity, is preserved.
Seeds suitable for direct consumption are usually treated under such conditions that all germination capacity, if present at all, is completely lost. In this context, reference can be made to, for instance, international patent application WO 96/20606, in which seeds, herbs and spices are sterilized by means of a heat treatment which may, for instance, be carried out with steam. While the taste and smell of the seeds which are subjected to such a treatment are well preserved, the germination capacity of seeds which are subjected to such a treatment is completely lost.
Seeds whose germination capacity needs to be preserved, by contrast, are nowadays treated with chemicals by, means of which the number of pathogens on the surface of the seeds is reduced, while the germination capacity can indeed be preserved. However, particularly for biological cultivation, and for seeds which are consumed or processed when germinated, the use of such chemical a is highly undesired, while the norms

regarding chemical additions for traditional seed production are becoming increasingly stringent.
The object of the present invention is therefore to provide a method for treating seeds, in which the seeds are sterilized without chemicals needing to be used, and in which at least a considerable extent of the germination capacity of the seeds is preserved or even increases.
It has now surprisingly been found that this can he achieved by a combination of a specific sterilization step and a specific cooling step.
The invention thus relates to a method for treating seeds, comprising the following steps:
(a) a sterilization step in which, for 0,1 to 60 seconds and at a minimum
pressure of 0.5 bar absolute, the seeds are contacted with a medium having
a condensation temperature of 80-200°C, wherein the medium condenses on
the seeds and the temperature of the surface of the seeds is raised to 200°C
at most; and
(b) a cooling step in which the sterilized seeds obtained in step (a) are
cooled, wherein the temperature of the surface of the seeds is lowered,
within 30 seconds at most, to 50°C at most, and the conditions of steps (a)
and (b) are set such that the germination capacity of the seeds is relatively
preserved or increases.
The seeds obtained with the method according to the invention exhibit a long storage life, while the germination capacity is at least to a considerable extent preserved or even increases. Very surprisingly, it has been observed that the seeds treated with the method according'to the invention often cave a better germination capacity than untreated seeds-
In the context of the present invention & "relative preservation of germination capacity" is understood to mean that the germination capacity as such is usually preserved, but that, in certain cases, the germination capacity can decrease by 25% at most, that is, a minimum preservation of 75% of the germination capacity such as it is present before the treatment.

In the use of the present invention, the condensing medium condenses on. the surface of cold seed in step (a), the surface being very rapidly and uniformly heated, after which, before the heat can penetrate the seed, in step (b), the formed condensate rapidly evaporates so that the surface of the seed is cooled.
The condensing medium may, for instance, consist of steam, but other media having a condensation temperature of 80-200°C are also suitable.
A skilled person will understand that the parameters to be selected for step (a), such as time, pressure and temperature, depend on the size of the seeds, the type of seed and the properties of the condensing medium. For instance, a certain type of seed having larger dimensions may usually be exposed to a steam treatment longer, while preserving germination capacity, than a type of seed having smaller dimensions.
Step (a) is carried out for 0.1-60 seconds, preferably for 0.1-5 seconds, and still more preferably for 0.5-1.5 seconds. In step (a), the surface of the seeds will teach a temperature of 200°C at most, before the seed parts are subjected to step (b). Preferably, in step (a), the surface of the seeds reaches a temperature of 80-150°C.
Step (a) is preferably carried out at a pressure of 0,5-20 bar absolute, still more preferably a pressure of 1-6 bar absolute.
Preferably, in step (b), the seeds are cooled by means of a vacuum treatment or another manner in which rapid evaporation occurs,
Step (b) is carried out such that, within 30 seconds, the temperature of the surface of the seeds is lowered to 50°C at most. Preferably, step (b) is carried out such that, within 10 seconds, the temperature of the surface of the seeds is lowered to 30°C at most.
Preferably, step (b) is carried out for 0.5-30 seconds, more preferably for 0.5-5 seconds, and still more preferably for 0.5-1 seconds. In step (a), preferably, steam is used as the condensing medium. The condensing medium may optionally be superheated.

The initial temperature of the seeds is preferably -30-30°C, more preferably 0-80°C and still more preferably 1O-26qC.
For the sake of completeness, it is noted that all above-mentioned limit ranges comprise both the limit values mentioned above and below.
It will be clear to a skilled person that the initial temperature of the seeds is understood to mean the temperature of the seeds just before or at the moment when they are subjected to the sterilization step. Preferably, the method according to the invention is carried out such that the temperature of the germinating part of the seeds does not greatly exceed the initial temperature of the seeds.
Preferably, the initial temperature of the germinating part of the seeds is essentially preserved. Although a small increase of the initial temperature will not directly have a great adverse influence on the germination capacity of the seeds, and sometimes even a positive influence, it will be clear that the germination capacity of the seeds will increasingly be harmed according as the temperature of the germinating part of the seeds more greatly exceeds the initial temperature during the treatment. The harm to the germination capacity depends on the maximum temperatures reached by the germinating parts of the seeds and the time during which these temperatures occur, This will differ per type of seed.
By means of the method according to the present invention, also, seeds can be treated which are suitable for consumption or industrial processing,
The seeds to be treated with the method, according to the invention are those plant parts which can be used for reproduction and can be chosen from the group of seed kernels, bulbs, tubers, cuttings and rhizomes, but are not limited to this group. The invention further relates to seeds which can be obtained by means of the method according to the invention.

Example 1
In experiments 1-10, barley seeds were treated with the method according to the invention. The duration and pressure in steps (a) and (b) were varied, and these are shown in Table 1. In experiments 1-5, the initial temperature of the cores of the seeds was 20°C, while in experiments 6-10 the initial temperature of the cores of the seeds was -20°C. In all experiments, the minimum supply temperature of the steam waa 120°C, and froth experiment 5, the steam was superheated to 160°C, It was then determined to what extent the treatment according to the invention had influenced the number of microorganisms on the seed, and what the effect on the germination of the seed was. As a control, also, the contents of microorganisms and the germination were determined of barley seeds which were Not subjected to the treatment according to the invention, and whose initial temperature was 20°C and -20°C, respectively.
Table1
(Table Removed)
After each treatment, the seeds (8g, approximately 60 seeds) were shaken for 3 hours with 10 ml of sterile demineralized water. Subsequently, 100 µl of a 1:1000 dilution of the water was spread on an LB plate and, for two days, incubation of the LB plates took place in an incubator at a temperature of 37°C. After this, the seeds obtained with the treatments were placed to germinate in Petri dishes between 2x2 layers of filter paper (with 3 and 2.5 ml of water, respectively). Incubation in the dark took place at 20°C for one week. Figs. 1 and 2 show the results of the number of colonies that could be counted on the Petri dishes and the maximum number of seeds that germinated after each treatment. Fig. 1 dearly shows that the number of microorganisms on the seeds decreased when the seeds were treated with the method according to the invention, while Fig. 2 clearly shows that the treatment according to the invention had a positive influence on the germination capacity of the seeds when the treatment took place for a relatively short time.

WE CLAIM:
1. A method for treating seeds, comprising the following steps:
(a) a sterilization step wherein the seeds have an initial temperature of -30°C to 30° C, that they are contacted with steam for 0.1 to 5 seconds, and at a minimum pressure of 0.5 bar absolute, the seeds are contacted with a medium having a condensation temperature of 80-200°C , wherein the medium condenses on the seeds and the temperature of the surface of the seed is raised to 200 ° C at most; and
(b) a cooling step wherein the sterilized seeds obtained in step (a) cooled, wherein the temperature of the surface is lowered within 10 seconds to 30° C at most, and wherein the conditions of steps (a) and (b) are set such that the germination capacity of the seeds is relatively preserved or increases.

2. A method as claimed in claim 1, wherein the step (a) is carried out for 0.1-5 seconds.
3. A method as claimed in claim 2, wherein step (a) is carried out for 0.5-1.5 seconds.
4. A method as claimed any one of claims 1 to 3, wherein, is step (a), the surface of the seeds reaches a temperature of 80-150° C, before the seeds are subjected to step (b).
5. A method as claimed in any one of claims 1 to 4, wherein the step (a) is carried out at a pressure of 0.5-20 bar absolute.
6. A method as claimed in any one of claims 1 to 5, wherein in step (b) the seeds are cooled by means of a vacuum treatment or another manner in which rapid evaporation occurs.
7.. A method as claimed in any one of claims 1 to 6, wherein step (b) is carried out such that, within 10 seconds, the temperature of the surface of the seeds is lowered to 30 ° C at most.
8. A method as claimed n claim 7, wherein step (b) is carried out for 0.5-5 seconds.
9. A method as claimed in any one of claims 1 to 8, wherein, in step (a), steam is used as the condensing medium.
10. A method as claimed in any one of claims 1 to 9 wherein the seeds have an initial temperature of 0-30° C.
11. A method as claimed in any one of claims 1 to 10, wherein the seeds have been chosen from the group of seed kernels, bulbs, tubers, cuttings, and rhizomes.

Documents:

3441-DELNP-2005-Abstract-(18-07-2008).pdf

3441-delnp-2005-abstract.pdf

3441-DELNP-2005-Claims-(12-02-2009).pdf

3441-DELNP-2005-Claims-(12-03-2009).pdf

3441-DELNP-2005-Claims-(18-07-2008).pdf

3441-delnp-2005-claims.pdf

3441-delnp-2005-complete specification (granted).pdf

3441-DELNP-2005-Correspondence-Others-(18-07-2008).pdf

3441-delnp-2005-correspondence-others.pdf

3441-delnp-2005-description (complete)-18-07-2008.pdf

3441-delnp-2005-description (complete).pdf

3441-delnp-2005-drawings.pdf

3441-DELNP-2005-Form-1-(18-07-2008).pdf

3441-delnp-2005-form-1.pdf

3441-delnp-2005-form-13.pdf

3441-delnp-2005-form-18.pdf

3441-DELNP-2005-Form-2-(18-07-2008).pdf

3441-delnp-2005-form-2.pdf

3441-DELNP-2005-Form-3-(18-07-2008).pdf

3441-delnp-2005-form-3.pdf

3441-DELNP-2005-Form-5-(18-07-2008).pdf

3441-delnp-2005-form-5.pdf

3441-DELNP-2005-GPA-(18-07-2008).pdf

3441-delnp-2005-gpa.pdf

3441-delnp-2005-pct-101.pdf

3441-delnp-2005-pct-210.pdf

3441-delnp-2005-pct-237-(18-07-2008).pdf

3441-delnp-2005-pct-304.pdf

3441-delnp-2005-pct-373-(18-07-2008).pdf

3441-delnp-2005-petition-137-(18-07-2008).pdf

3441-delnp-2005-petition-138-(18-07-2008).pdf

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Patent Number

233094

Indian Patent Application Number

3441/DELNP/2005

PG Journal Number

13/2009

Publication Date

27-Mar-2009

Grant Date

26-Mar-2009

Date of Filing

03-Aug-2005

Name of Patentee

NEDERLANDSE ORGANISATIE VOOR TOEGEPAST - NATUURWETENSCHAPPELIJK ONDERZOEK TNO,

Applicant Address

SCHOEMAKERSTRAAT 97, 2628 VK DELT, THE NETHERLANDS,

Inventors:

#	Inventor's Name	Inventor's Address
1	HENDRICUS CORNELIS VAN DEVENTER,	KRUYDERSTRAAT 7, 7312 TA APELDOORN, NETHERLAND
2	ALBERT VAN DUIJN,	RHIJNGEESTERSTRAATWEG 52, 2341 BV OEGSTGEEST, THE NETHERLANDS
3	RUDY MATHIAS HENRICUS HEIJMANS	DE JANSWEI 16, 6932 MT WESTERVOORST, NETHERLANDS

PCT International Classification Number

A01C 1/08

PCT International Application Number

PCT/NL2004/000066

PCT International Filing date

2004-01-29

PCT Conventions:

#	PCT Application Number	Date of Convention	Priority Country
1	1022549	2003-01-31	Netherlands