Indian Patents. 269171:METHODS FOR STABILIZING OXIDATIVELY UNSTABLE COMPOSITIONS

Title of Invention	METHODS FOR STABILIZING OXIDATIVELY UNSTABLE COMPOSITIONS
Abstract	Ophthalmic compositions and methods of preparing such compositions are disclosed.

Full Text	METHODS FOR STABILIZING OXIDATIVELY UNSTABLE COMPOSITIONS FIELD OF THE INVENTION This invention related to compositions of oxidatively unstable ophthalmic ingredients and methods for preparing such compositions RELATED APPLICATION This application claims priority from a non-provisional filing, U.S. App. Pat. No. U.S. App. Pat. No. 60/783,557 entitled "Methods for Stabilizing Oxidatively Unstable Pharmaceutical Compositions," which was filed on, March 17, 2006. BACKGROUND Therapeutic agents for topical administration to the eye are generally formulated in either a liquid or gel form and must be kept sterile until administration. Accordingly, ophthalmic therapeutic agents are either i packaged asceptically, which is cumbersome and expensive or are heat sterilized. Unfortunately, many therapeutic agents are not oxidatively stable, especially at elevated temperatures. EDTA, Dequest, and Desferal have been used to improve the stability of certain therapeutic agents during autoclaving. However, there remains a need for other compounds capable of stabilizing unstable therapeutic agents that are susceptible to oxidative degradation. This need is met by the following invention. BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 Stability Study with Ketotifen and PAA or EDTA Fig. 2. Stability Study with Ketotifen and PAA or EDTA Fig. 3 Stability Study with Ketotifen and PAA or DTPA DETAILED DESCRIPTION OF THE INVENTION This invention includes a method of stabilizing an ophthalmic composition comprising an oxidatively unstable pharmaceutical ingredient wherein said method comprises adding an effective amount of a stabilizing agent to the ophthalmic composition. As used herein "oxidatively unstable pharmaceutical ingredient" refers to pharmaceutical or nutraceutical compounds used to treat conditions of the eye, and such compound degrade in the presence of oxygen and certain transition metals. Examples of pharmaceutical compounds include antihistamines, antibiotics, antibacterial agents, antiviral agents, antifungal agents, analgesics, anesthetics, antiallergeneic agents, mast cell stabilizers, steroidal and non- steroidal anti-inflammatory agents, angiogenesis inhibitors; antimetabolites, fibrinolytics, neuroprotective drugs, angiostatic steroids, mydriatics.cyclopegic mydriatics; miotics; vasoconstrictors; vasodilators, anticlotting agents; anticancer agents, antisense agents, immunomodulatory agents, carbonic anhydrase inhibitors, integrin antabonistsl; cyclooxygenase inhibitors, VEGF antagonists; immunosuppressant agents and the like. Particularly, examples of pharmaceutical agents include but are not limited to acrivastine, antazoline, i astemizole, azatadine, azelastine, buclizine, bupivacaine, cetirizine, clemastine, cyclizine, cyproheptadine, ebastine, emedastine, ephedrine, eucatropine, i fexofenadine, homatropine, hydroxyzine, ketotifen, levocabastine, levoceterizine, lomefloxacin, meclizine, mepivacaine, mequitazine, methdilazine, methapyrilene, mianserin, naphazoline norastemizole, i norebastine, ofloxacin, oxymetazoline, pheniramine, phenylephrine, physostigmine, picumast, promethazine, scopolamine, terfenadine, tetrahydozoline, thiethylperazine, timolol, trimeprazine, triprolidine, pharmaceutically acceptable salts and mixtures thereof. Preferred pharmaceutical compounds include acrivatine, antazoline, astemizole, azatadine, azelastine, clemastine, cyproheptadine, ebastine, emedastine, eucatropine, fexofenadine, homatropine, hydroxyzine, ketotife, levocabastine, levoceterizine, meclizine, mequitazine, methdialazine, methapyrilene, norastemizole, norebastine, oxymetazoline, physootigmine, picumast, promethazine, scopolamine, terfenadine, tetrahyerozoline, fimilol, trimeprazine, triprolidine, and pharmaceutically acceptable salts thereof. Particularly preferred pharmaceutical compounds include phenarimine, ketotifen, ketotifen fumarate nor ketotifen.olapatadine and mixtures thereof. More particularly preferred pharmaceutical compounds include ketotifen, its pharmaceutically acceptable salts, and mixtures thereof. Examples of nutraceutical compounds include vitamins and supplements such as vitamins A, D, E, lutein, zeaxanthin, lipoic acid, flavonoids, ophthalmicially compatible fatty acids, such as omega 3 and omega 6 fatty acids, combinations thereof, combinations with pharmaceutical compounds and the like. Preferred pharmaceutical or nutraceutical compounds are those that degrade when solutions of these compounds and oxidative catalysts (such as metals and metallic salts) are mixed together at ambient or elevated temperatures, as compared to solutions of these compounds without oxidative catalysts at ambient or elevated temperatures. Particularly preferred pharmaceutical or nutraceutical compounds are those that degrade greater than about 10% when heated to about 120°C for about 20 minutes with oxidative catalysts. The concentration of oxidatively unstable pharmaceutical ingredients in the ophthalmic compositions of the invention range from about 2 μg/mL to about 6.5 g/mL, particularly preferred, about 0.1 μg/mL to about 10,000 μg/mL. The term "ophthalmic composition" refers to liquids, aerosols, or gels that may be topically administered to the eye. The term "stabilizing agent" refers to chelant compositions that inhibit metal catalyzed oxidative degradation of the oxidatively unstable pharmaceutical ingredient. Examples of stabilizing agents include but are not limited to silica, chitin derivative such as chitosan, polyamides such as poly(aspartic acid-co-w-amino acid (See CAN:129:54671, Shibata, Minako et al. Graduate School Environmental Earth Science, Hokkaido University, Sapporo, Japan Macromolecular Symposia (1998), 130, 229-244) and polymeric amides such as poly[iminocarbonyl(2,5-dihydroxy- 1,4-phenylene)carbonylimino-1,4-phenylenemethylene-1,4-phenylene], CAS # 87912-00-3, polymeric lactams such as polyvinylpyrrolidone, polyamino carboxylic acids such as diethylenetriaminepentaacetic acid and triethylenetriaminepentaacetic acid, polymeric amines such as polyallylamine, i crown ethers such as 18-crown-6, 21-crown-7, and 24-crown-8, cellulose and its derivatives, and N,N,N',N',N", N"-hexa(2-pyridyl)-1,3,5- tris(aminomethyl)benzene, and certain macrocyclic ligands such as crown ethers, ligand containing knots and catenands (See, David A. Leigh et al Angew. Chem Int. Ed., 2001, 40, No. 8, pgs. 1538-1542 and Jean-Claude Chambron et al. Pure & Appl. Chem., 1990, Vol. 62, No. 6, pgs. 1027-1034) The preferred stabilizing agents are polyamino carboxylic acids such as diethylenetriaminepentaacetic acid and triethylenetriaminepentaacetic acidi The particulary preferred stabilizing agents are diethylenetriaminepentaacetic acid ("DTPA"), or salts of DTPA such as CaNa3DTPA, ZnNa3DTPA, and Ca2DTPA. The term "effective amount" refers to the amount of stabilizing agent required to inhibit the oxidative degradation of the pharmaceutical ingredient. In most circumstances it is preferred that there is a 1:1 molar ration of metal present in the ophthalmic composition to chelant, is more preferably about 1 of metal to greater than about 1 of chelant compositions, most preferably about 1 of metal to greater than or equal to about 2 of chelant compositions. With respect to concentration limits, it is preferred that the stabilizing agents have a concentration in the ophthalmic composition from about 2.5 μmoles/liter to about, 5000 μmoles/liter more preferably from about 20 μmoles/liter to about 1000 μmoles/liter, more preferably from about 100 μmoles/liter to about 1000 μmoles/liter, most preferably from about 100 μmoles/liter to about 500 μmoles/liter. Aside from the oxidatively unstable pharmaceutical ingredient and the stabilizing agent, the ophthalmic composition contains suitable ophthalmic carriers. Suitable carriers include antioxidants (radical scavengers), demulcents, antibacterial agents, solubilizers, surfactants, buffer agents, tonicity adjusting agents, chelating agents, preservatives, wetting agents, thickeners, water, saline solution, mineral oil, petroleum jelly, water soluble solvents, such as C15-20 alcohols, C15-20 amides, C15-20 alcohols substituted with zwitterions, vegetable oils or mineral oils comprising from 0.5 to 5% by weight hydroxyethylcellulose, ethyl oleate, carboxymethylcellulose, polyvinyl- pyrrolidone and other non-toxic water-soluble polymers for ophthalmic uses, such as, for example cellulose derivatives, such as methylcellulose, alkali metal salts of carboxy-methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, methylhydroxypropyl-cellulose, hydroxypropylcellulose, chitosan and scleroglucan, acrylates or methacrylates, such as salts of poly(acrylic acid) or ethyl acrylate, polyacrylamides, natural products, such as gelatin, alginates, pectins, tragacanth, karaya gum, xanthan gum, carrageenin, agar and acacia, starch derivatives, such as starch acetate and hydroxypropyl starch, and also other synthetic products, such as poloxamers, e.g. Poloxamer F127, polyvinyl alcohol, polyvinylpyrrolidone, polyvinyl methyl ether, polyethylene oxide, preferably cross-linked poly(acrylic acid), such as neutral Carbopol, or mixtures of those polymers. Preferred carriers are water, cellulose derivatives, such as methylcellulose, alkali metal salts of carboxymethylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, methylhydroxypropylcellulose and hydroxypropylcellulose, neutral Carbopol, or mixtures thereof. The concentration of the carrier is, for example, from 0.1 to 100000 times the concentration of the active ingredient combinations thereof and the like. When the ophthalmic composition is an eye drop, preferred carriers include water, pH buffered saline solution, mixtures thereof and the like. The preferred carrier is an aqueous saline solution containing salts including, without limitation, sodium chloride, sodium borate, sodium phosphate, sodium hydrogenphosphate, sodium dihydrogenphosphate, or the corresponding potassium salts of the same. These ingredients are generally combined to form buffered solutions that include an acid and its conjugate base, so that addition of acids and bases cause only a relatively small change in pH. The buffered solutions may additionally include 2-(N- morpholino)ethanesulfonic acid (MES), sodium hydroxide, 2,2- bis(hydroxymethyl)-2,2',2"-nitrilotriethanol, n-tris(hydroxymethyl)methyl-2- aminoethanesulfonic acid, citric acid, sodium citrate, sodium carbonate, sodium bicarbonate, acetic acid, sodium acetate, ethylenediamine tetraacetic acid and the like and combinations thereof. Most preferably, the carrier is a borate buffered or phosphate buffered saline solution. Further the invention includes an ophthalmic composition comprising an oxidatively unstable pharmaceutical ingredient and an effective amount of a stabilizing agent. The terms oxidatively unstable pharmaceutical ingredient, effective amount, and stabilizing agents all have their aforementioned meanings and preferred ranges. Still further the invention includes a method of stabilizing an ophthalmic composition comprising an oxidatively unstable excipient wherein said method comprises adding an effective amount of a stabilizing agent to the ophthalmic i composition. As used herein "oxidatively unstable excipient" refers to a component of ophthalmic compositions that degrades in the presence of oxygen and certain transition metals. Examples of unstable excipients include but are not limited to astringents, demulcents, emollients, hypertonicity agents, oleaginous, agents, tonicity agents mucomimetic agents, and the like. Particularly examples of unstable excipients include but are not limited to cellulose derivatives, carboxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, hyaluronic acid, methylcellulose, Dextran, gelatin, polyols, glycerin, polyethylene glycol, polysorbate, propylene glyxol, polyvinyl alcohol, povidone lanolin, mineral oil, paraffin, petrolatum, white ointment, white petrolatum, white, wax, and yellow wax. The terms "stabilizing agent," and "effective amount" have their aforementioned meanings and preferred ranges. i Yet still further the invention includes an ophthalmic composition i comprising an oxidatively unstable excipient and an effective amount of a stabilizing agent. The terms "oxidatively unstable excipient," "stabilizing agent," and "effective amount" have their aforementioned meanings and preferred ranges. Even further still the invention includes a method of stabilizing an ophthalmic composition comprising an oxidatively unstable pharmaceutical ingredient wherein said method comprises (a) functionalizing said stabilizing agent with a polymerizable group, (b) polymerizing an effective amount of the product of step (a) with at least one type of polymerizable monomer, and (c) contacting the ophthalmic composition comprising an oxidatively unstable pharmaceutical ingredient with the polymer of step (b). The terms oxidatively unstable pharmaceutical ingredient, effective amount, and stabilizing agents all have their aforementioned meanings and preferred ranges. The term functionalizing means chemically bonding a polymerizable group to said stabilizing agent. Examples of a polymerizable group includes but are not limited to methacrylate, acrylate, acrylamide, and styrene. The term polymerizable monomer includes compounds containing olefinic moieties capable of adding to radical species, such as propylene, ethylene and the like. The polymerization product of step (b) may be soluble or insoluble in the ophthalmic composition. It is preferred that the polymerization product of step (b) is not soluble in the ophthalmic composition and as such the polymerization product of step (b) may be in any form such as rods, discs, containers, films and the like. Even further still the invention includes a method of stabilizing an ophthalmic composition comprising an oxidatively unstable excipient wherein said method comprises (a) functionalizing said stabilizing agent with a polymerizable group, (b) polymerizing an effective amount of the product of step (a) with at least one type of polymerizable monomer, and (c) contacting the ophthalmic composition comprising an oxidatively unstable exicipient with the polymer of step (b). i The terms oxidatively unstable excipient, effective amount, stabilizing agents, polymerizable group, and polymerizable monomers all have their aforementioned meanings and preferred ranges. Further still the invention includes a container for an ophthalmic composition comprising an oxidatively unstable pharmaceutical ingredient wherein said container comprises the polymerization product of an effective amount of a stabilizing agent functionalized with a polymerizable group and at least one type of polymerizable momoner. The terms oxidatively unstable pharmaceutical ingredient, effective amount, stabilizing agents, polymerizable group, and polymerizable monomers all have their aforementioned meanings and preferred ranges. Yet, further still the invention includes a container for an ophthalmic composition comprising an oxidatively unstable excipient wherein said container comprises the polymerization product of an effective amount of a stabilizing agent functionalized with a polymerizable group and at least one type of polymerizable momoner. The terms oxidatively unstable excipient, effective amount, stabilizing agents, polymerizable group, and polymerizable monomers all have their aforementioned meanings and preferred ranges. The advantages of the compositions and methods of this invention are numerous. First ketotifen is known as a oxidatively unstable pharmaceutical i ingredient. Compositions containing ketotifen fumarate are known. These compositions contain EDTA and the pH of those compositions is about 5.5. These EDTA solutions stabilize the ketotifen fumarate against oxidative degradation, the pH of these solutions is below the threshold for ocular awareness and it is likely that some patient who use this solution will be uncomfortable due to low pH value of the solution. See Tang, I., Wong, D.M., Yee, D.J. and Harris, M.G. 1996 The pH of multi-purpose soft contact lens solutions. Optom. Vis. Sci. 73:746-749. Adler, F.H. 1959 Physiology of the Eye. Third edition, p.40. Brawner, L.W. and Jessop, D.G. 1962. A review of contact lens solutions. Contacto 6:49-51. It has been discovered that the stabilizing agents of the invention will reduce the degradation of ketotifen fumarate at higher pH values that those of ketotifen fumarate solutions containing EDTA. The pH of ophthalmic compositions of the invention is preferably between about pH 6.6 and about pH 7.2, more preferably between about pH 6.8 and about pH 7.0. Second it has been shown that the shelf life of ketotifen fumarate i solutions containing DTPA are superior to the shelf life of ketotifen fumarate solutions containing either EDTA or PAA. Third, it is known that the application of heat increases the rate of degradation of many pharmaceutical ingredients. i It has been shown that the stabilizing agents of this invention are useful in reducing degradation associated with higher temperature, such as sterilization temperatures. In order to illustrate the invention the following examples are included. These examples do not limit the invention. They are meant only to suggest a i method of practicing the invention. Those knowledgeable in contact lenses as well as other specialties may find other methods of practicing the invention. However, those methods are deemed to be within the scope of this invention. EXAMPLES The following abbreviations are used below PAA Polyacrylic Acid, sodium salt having an average molecular weight of 200,000 EDTA Ethylenediamine tetraacetic acid. Solution A Deionized water containing the following ingredients by weight: NaCI (0.83%), Boric Acid (0.91%), Sodium tetraborate decahydrate (0.1%) Initial Testing to Determine Suitability of Ingredients It is known that excessive quantities of certain transition metals and their salts will degrade ketotifen fumarate. The amount of metals and salts contained within commercially available ingredients varies, so test batches of Solution A were evaluated as follows. 200 g Solution A was mixed at ambient temperature and ketotifen fumarate (5 mg + 2 mg) was added and mixed until homogenous. Six glass vials were filled with 3ml_ of this solution. The vials were stopped with poly tetrafluoroethylene ("PTFE ") and three of the vials were heated for eighteen minutes at 124 °C. Samples of each treated vial (1.0-1.5 mL) were analyzed by HPLC and compared to the untreated controls. If the amount of ketotifen in the treated vials reduced by less than or equal to five percent (≤5%) the ingredients were determined to be suitable for further studies and larger batches of Solution A were prepared from these ingredients. Example 1 Preparation of Ketotifen Fumarate Solutions with PAA. EDTA PAA (2000 μg/mL) was added to Solution A, and 50 μg/ml_ of ketotifen fumarate (approximately 36 μg/mL of ketotifen) was dissolved in the system. EDTA (100 μg/mL) was added to another batch of Solution A, and 50 μg/mL of ketotifen fumarate was dissolved in the system. Negative One and negative twelve (-1.0 and 12.0) diopter etafilcon A contact lenses were added to vials containing 3mL of each of the above solutions. The vials were sealed with PTFE coated rubber stoppers, sterilized at 124 °C for 18 minutes, and stored at ambient temperature for one year. Samples were harvested throughout the year and analyzed for the presence of ketotifen by HPLC. The results are presented in Figures 1 and 2. These results show that there is substantial degradation of the ketotifen over time with PAA and with EDTA. Example 2 Preparation of Ketotifen Fumarate Solutions with PAA. DTPA ; To separate batches of Solution A, Ca2DTPA (100 μg/mL and 300 μg/mL, 213 and 640 μmoles/L respectively) and 2000 μg/mL (0.2%, approximately 18.51 mmole/L methacrylate content) of PAA were added, and approximately 25 μg/mL of ketotifen fumarate (approximately 18 μg/mL of ketotifen) was dissolved in each of the systems. Three mL samples of each solution were added to individual vials containing contact lenses. Each set of vials was closed with PTFE stoppers, sterilized at 124 °C for 18 minutes, and stored at 80°C for a period of two weeks. Samples were harvested at various intervals and analyzed for the presence of ketotifen by HPLC. The results are presented in Figure 3. These results show that there is substantial degradation of the ketotifen over time with PAA but not with the calcium salt of DTPA Example 3 Ketotifen Fumarate Solutions with oxidation catalysts Ketotifen fumarate (50μg/ml_, approximately 36μg/mL of ketotifen) was dissolved in Solution A containing approximately 500 μg/mL of either DTPA (approximately 1272 μmoles/L of DTPA) or the sodium salt of EDTA (Na2C10H1408N2•2 H2O, 1344 μmoles/L of EDTA). Approximately 50 μg/mL of the salts listed in Table 1 were dissolved in each of the solutions and about 3ml_ of each solution was dosed into several vials The vials were sealed With PTFE coated rubber stoppers and were subjected to zero, one, two or three sterilization cycles as indicated in Table 1. One sterilization cycle is eighteen minutes of heating at 124 °C. Samples were analyzed by HPLC for the concentration of ketotifen at the intervals indicated in Table 2. This data shows that in the presence of oxidative catalysts, DTPA reduces the amount of oxidative degradation as compared to EDTA. What is claimed is 1. A method of stabilizing an ophthalmic composition comprising an oxidatively unstable pharmaceutical ingredient wherein said method comprises adding an effective amount of a stabilizing agent to the ophthalmic composition. 2. The method of claim 1 wherein the stabilizing agent is selected from the group consisting of silica, chitin derivative such as chitosan, cellulose its derivatives, and N,N,N',N',N", N"-hexa(2-pyridyl)-1,3,5- tris(aminomethyl)benzene, diethylenetriaminepentaacetic acid, and salts of diethylenetriaminepentaacetic. 3. The method of claim 1 wherein the stabilizing agent is selected from the group conisiting of diethylenetriaminepentaacetic acid, and salts of diethylenetriaminepentaacetic acid. 4. The method of claim 1 wherein the effective amount of the stabilizing agent is about 2.5 μmoles/liter to about 5000 μmoles/liter. 5. The method of claim 1 wherein the effective amount of the stabilizing agent is about 20 μmoles/liter to about 1000 μmoles/liter. 6. The method of claim 1 wherein the effective amount of the stabilizing agent is about 100 μmoles/liter to about 600 μmoles/liter. 7. The method of claim 1 wherein the stabilizing agent is diethylenetriaminepentaacetic acid. 8. The method of claim 1 wherein the stabilizing agent is diethylenetriaminepentaacetic acid or salts there of and the effective amount of said stabilizing agent is about 100 μmoles/liter to about 1000 μmoles/liter 9. The method of claim 1 wherein the oxidatively unstable pharmaceutical ingredient is selected from the group consisting of acrivastine, antazoline, astemizole, azatadine, azelastine, buclizine, bupivacaine, cetirizine, clemastine, cyclizine, cyproheptadine, ebastine, emedastine, eucatropine, fexofenadine, homatropine, hydroxyzine, ketotifen, levocabastine, levoceterizine, lomefloxacin, meclizine, mepivacaine, mequitazine, methdilazine, methapyrilene, mianserin, norastemizole, norebastine, ofloxacin, oxymetazoline, pheniramine, physostigmine, picumast, promethazine, scopolamine, terfenadine, tetrahydozoline, thiethylperazine, timolol, trimeprazine, triprolidine, pharmaceutically acceptable salts and mixtures thereof. 10. The method of claim 1 wherein the oxidatively unstable pharmaceutical ingredient is selected from the group consisting of acrivatine, antazoline, astemizole, azatadine, azelastine, clemastine, cyproheptadine, ebastine, emedastine, eucatropine, fexofenadine, homatropine, hydroxyzine, ketotifen, levocabastine, levoceterizine, meclizine, mequitazine, methdialazine, methapyrilene, norastemizole, norebastine, oxymetazoline, physootigmine, picumast, promethazine, scopolamine, terfenadine, tetrahyerozoline, fimilol, trimeprazine, triprolidine, and pharmaceutically acceptable salts thereof. 11. The method of claim 1 wherein the oxidatively unstable pharmaceutical ingredient is selected from the group consisting of phenarimine, ketotifen, i ketotifen fumarate, nor ketotifen fumarate olopatadine and mixtures thereof. 12. The method of claim 1 wherein the oxidatively unstable pharmaceutical ingredient is selected from the group consisting of ketotifen, its pharmaceutically acceptable salts, and mixtures thereof. i 13. The method of claim 1 wherein the oxidatively unstable pharmaceutical ingredient is selected from the group consisting of vitamins A, D, E, lutein, zeaxanthin, lipoic acid, flavonoids, ophthalmicially compatible fatty acids, such as omega 3 and omega 6 fatty acids and combinations thereof. 14. The method of claim 1 wherein the stabilizing agent is diethylenetriaminepentaacetic acid or salts there of, the effective amount of said stabilizing agent is about 100 μmoles/liter to about 1000 μmoles/liter, and the oxidatively unstable pharmaceutical ingredient is selected from the group consisting of ketotifen, its pharmaceutically acceptable salts, and mixtures thereof. 15. The method of claim 1 wherein the stabilizing agent is the calcium salt diethylenetriaminepentaacetic acid, the effective amount of said stabilizing agent is about 100 μmoles/liter to about 1000 μmoles/liter, and the oxidatively unstable pharmaceutical ingredient is selected from the group consisting of ketotifen, pharmaceutically acceptable salts of ketotifen and mixtures thereof. 16. The method of claim 1 wherein the ophthalmic composition has a pH of about 6.6 to about 7.2. 17. The method of claim 1 wherein the ophthalmic composition has a pH of about 6.8 to about 7.2. 18. An ophthalmic composition comprising an oxidatively unstable pharmaceutical ingredient and an effective amount of a stabilizing agent. 19. The ophthalmic composition of claim 18 wherein the stabilizing agent is selected from the group consisting of silica, chitin derivative such as chitosan, cellulose its derivatives, and N,N,N',N',N", N"-hexa(2-pyridyl)-1,3,5- tris(aminomethyl)benzene, diethylenetriaminepentaacetic acid, and salts of diethylenetriaminepentaacetic acid, and acid. 20. The ophthalmic composition of claim 18 wherein the stabilizing agent is selected from the group conisiting of diethylenetriaminepentaacetic acid, and salts of diethylenetriaminepentaacetic acid. 21. The ophthalmic composition of claim 18 wherein the effective amount of the stabilizing agent is about 2.5 μmoles/liter to about 5000 μmoles/liter. 22. The ophthalmic composition of claim 18 wherein the effective amount of the stabilizing agent is about 100 μmoles/liter to about 1000 μmoles/liter. 23. The ophthalmic composition of claim 18 wherein the effective amount of the stabilizing agent is about 100 μmoles/liter to about 600 urnoles/liter. 24. The ophthalmic composition of claim 18 wherein the stabilizing agent is diethylenetriaminepentaacetic acid 25. The ophthalmic composition of claim 18 wherein the stabilizing agent is diethylenetriaminepentaacetic acid or salts there of and the effective amount of said stabilizing agent is about 100 μmoles/liter to about 1000 μmoles/liter 26. The ophthalmic composition of claim 18 wherein the oxidatively unstable pharmaceutical ingredient is selected from the group consisting of acrivastine, antazoline, astemizole, azatadine, azelastine, buclizine, bupivacaine, cetirizine, clemastine, cyclizine, cyproheptadine, ebastine, emedastine, eucatropine, fexofenadine, homatropine, hydroxyzine, ketotifen, levocabastine, levoceterizine, lomefloxacin, meclizine, mepivacaine, mequitazine, methdilazine, methapyrilene, mianserin, norastemizole, norebastine, ofloxacin, oxymetazoline, pheniramine, physostigmine, picumast, promethazine, scopolamine, terfenadine, tetrahydozoline, thiethylperazine, timolol, trimeprazine, triprolidine, pharmaceutically acceptable salts and mixtures thereof. 27. The ophthalmic composition of claim 18 wherein the oxidatively unstable pharmaceutical ingredient is selected from the group consisting of acrivatine, antazoline, astemizole, azatadine, azelastine, clemastine, cyproheptadine, ebastine, emedastine, eucatropine, fexofenadine, homatropine, hydroxyzine, ketotifen, levocabastine, levoceterizine, meclizine, mequitazine, methdialazine, methapyrilene, norastemizole, norebastine, oxymetazoline, physootigmine, picumast, promethazine, scopolamine, terfenadine, tetrahyerozoline, fimiloj, trimeprazine, triprolidine, and pharmaceutically acceptable salts thereof. 28. The ophthalmic composition of claim 18 wherein the oxidatively unstable pharmaceutical ingredient is selected from the group consisting of phenarimine, ketotifen, ketotifen fumarate nor ketotifen fumarate, olopatadine and mixtures thereof. 29. The ophthalmic composition of claim 18 wherein the oxidatively unstable pharmaceutical ingredient is selected from the group consisting of ketotifen, its pharmaceutically acceptable salts, and mixtures thereof. 30. The ophthalmic composition of claim 18 wherein the oxidatively unstable pharmaceutical ingredient is selected from the group consisting of vitamins A, D, E, lutein, zeaxanthin, lipoic acid, flavonoids, ophthalmicially compatible fatty acids, such as omega 3 and omega 6 fatty acids and combinations thereof. 31. The ophthalmic composition of claim 18 wherein the stabilizing agent is diethylenetriaminepentaacetic acid or salts there of, the effective amount of said stabilizing agent is about 200 μmoles/liter to about 1000 μmoles/liter, and the oxidatively unstable pharmaceutical ingredient is selected from the group consisting of ketotifen, its pharmaceutically acceptable salts, and mixtures thereof. 32. The ophthalmic composition of claim 18 wherein the stabilizing agent is the calcium salt diethylenetriaminepentaacetic acid, the effective amount of said stabilizing agent is about 100 μmoles/liter to about 1000 μmoles/liter, and the oxidatively unstable pharmaceutical ingredient is selected from the group consisting of ketotifen, pharmaceutically acceptable salts of ketotifen and mixtures thereof. 33. The ophthalmic composition of claim 18 wherein the pH is about 6.6 to about 7.2. 34. The ophthalmic composition wherein the pH is about 6.8 to about 72. 35. A method of stabilizing an ophthalmic composition comprising an oxidatively unstable excipient wherein said method comprises adding an effective amount of a stabilizing agent to the ophthalmic composition. 36. The method of claim 35 wherein the oxidatively unstable excipient is selected from the group consisting of cellulose derivatives, carboxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, hyaluronic acid, methylcellulose, Dextran, gelatin, polyols, glycerin, polyethylene glycol, polysorbate, propylene glyxol, polyvinyl alcohol, povidone lanolin, mineral oil, paraffin, petrolatum, white ointment, white petrolatum, white, wax, and yellow wax. 37. The method of claim 35 wherein the stabilizing agent is selected from the group consisting of silica, chitin derivative such as chitosan, cellulose its derivatives, and N,N,N\N',N", N"-hexa(2-pyridyl)-1,3,5- tris(aminomethyl)benzene, diethylenetriaminepentaacetic acid, and salts of diethylenetriaminepentaacetic acid. 38. The method of claim 35 wherein the stabilizing agent is selected from the group conisiting of diethylenetriaminepentaacetic acid, and salts of diethylenetriaminepentaacetic acid. 39. The method of claim 35 wherein the effective amount of the stabilizing agent is about 20 μmoles/liter to about 1000 μmoles/liter. 40. The method of claim 35 wherein the effective amount of the stabilizing agent is about 100 μmoles/liter to about 600 μmoles/liter. 41. The method of claim 35 wherein the stabilizing agent is diethylenetriaminepentaacetic acid. 42. The method of claim 35 wherein the stabilizing agent is diethylenetriaminepentaacetic acid or salts there of and the effective amount of said stabilizing agent is about 100 μmoles/liter to about 1000 \|jmoles/liter ! 43. The method of claim 35 wherein the stabilizing agent is the calcium salt diethylenetriaminepentaacetic acid, the effective amount of said stabilizing agent is about 100 μmoles/liter to about 1000 μmoles/liter, and the oxidatively unstable excipient is selected from the group consisting of carboxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, hyaluronic acid, methylcellulose, Dextran, gelatin, polyols, glycerin, polyethylene glycol, polysorbate, propylene glyxol, polyvinyl alcohol, and mixtures thereof. 44. The method of claim 35 wherein the ophthalmic composition has a pH of about 6.6 to about 7.2. 45. The method of claim 35 wherein the ophthalmic composition has a pH of about 6.8 to about 7.2. 46. An ophthalmic composition comprising an oxidatively unstable excipient and an effective amount of a stabilizing agent. 47. The ophthalmic composition of claim 46 wherein the stabilizing agent is selected from the group consisting of silica, chitin derivative such as chitosan, cellulose its derivatives, and N.N.N'.N'.N", N"-hexa(2-pyridyl)-1,3,5- tris(aminomethyl)benzene, diethylenetriaminepentaacetic acid, and salts of diethylenetriaminepentaacetic acid, and acid. 48. The ophthalmic composition of claim 46 wherein the stabilizing agent is selected from the group conisiting of diethylenetriaminepentaacetic acid, and salts of diethylenetriaminepentaacetic acid. 49. The ophthalmic composition of claim 46 wherein the effective amount of the stabilizing agent is about 20 μmoles/liter to about 1000 μmoles/liter. 50. The ophthalmic composition of claim 46 wherein the effective amount of the stabilizing agent is about 100 μmoles/liter to about 600 μmoles/liter. 51. The ophthalmic composition of claim 46 wherein the stabilizing agent is i diethylenetriaminepentaacetic acid. 52. The ophthalmic composition of claim 46 wherein the stabilizing agent is diethylenetriaminepentaacetic acid or salts there of and the effective amount of said stabilizing agent is about 100 μmoles/liter to about 1000 μmoles/liter 53. The ophthalmic composition of claim 46 wherein the stabilizing agent is the calcium salt diethylenetriaminepentaacetic acid, the effective amount of said stabilizing agent is about 100 μmoles/liter to about 1000 μmoles/liter, and the oxidatively oxidatively unstable excipient is selected from the group consisting of carboxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, hyaluronic acid, methylcellulose, Dextran, gelatin, polyols, glycerin, polyethylene glycol, polysorbate, propylene glyxol, polyvinyl alcohol, and mixtures thereof. 54. The ophthalmic composition of claim 46 wherein the pH is about 6.6 to about 7.2. 55. The ophthalmic composition of claim 46 wherein the pH is about 6.8 to about 7.2. 56. A method of stabilizing an ophthalmic composition comprising an oxidatively unstable pharmaceutical ingredient wherein said method comprises (a) functionalizing said stabilizing agent with a polymerizable group, (b) polymerizing an effective amount of the product of step (a) with at least one type of polymerizable monomer, and (c) contacting the ophthalmic composition comprising an oxidatively unstable pharmaceutical ingredient with the polymer of step (b). 57. The method of claim 56 wherein the stabilizing agent is selected from the group consisting of silica, chitin derivative such as chitosan, cellulose its derivatives, and N.N.N'.N'.N", N"-hexa(2-pyridyl)-1,3,5- tris(aminomethyl)benzene, diethylenetriaminepentaacetic acid, and salts of diethylenetriaminepentaacetic acid, and acid. 58. The method of claim 56 wherein the stabilizing agent is selected from the group consisting of diethylenetriaminepentaacetic acid, and salts of diethylenetriaminepentaacetic acid. 59. A method of stabilizing an ophthalmic composition comprising an oxidatively unstable excipient wherein said method comprises (a) functionalizing said stabilizing agent with a polymerizable group, (b) polymerizing an effective amount of the product of step (a) with at least one type of polymerizable monomer, and (c) contacting the ophthalmic composition comprising an oxidatively unstable exicipient with the polymer of step (b). 60. The method of claim 59 wherein the stabilizing agent is selected from the group consisting of silica, chitin derivative such as chitosan, cellulose its derivatives, and N,N,N',N',N", N"-hexa(2-pyridyl)-1,3,5- tris(aminomethyl)benzene, diethylenetriaminepentaacetic acid, and salts of diethylenetriaminepentaacetic acid, and acid. 61. The method of claim 59 wherein the stabilizing agent is selected from the group consisting of diethylenetriaminepentaacetic acid, and salts of diethylenetriaminepentaacetic acid. 62. A container for an ophthalmic composition comprising an oxidatively unstable excipient wherein said container comprises the polymerization product of an effective amount of a stabilizing agent functionalized with a polymerizable group and at least one type of polymerizable momoner. 63. The container of claim 62 wherein the stabilizing agent is selected from the group consisting of silica, chitin derivative such as chitosan, cellulose its derivatives, and N,N,N',N',N", N"-hexa(2-pyridyl)-1,3,5- tris(aminomethyl)benzene, diethylenetriaminepentaacetic acid, and salts of diethylenetriaminepentaacetic acid, and acid. 64. The container of claim 62 wherein the stabilizing agent is selected from the group consisting of diethylenetriaminepentaacetic acid, and salts of diethylenetriaminepentaacetic acid. 65. A container for an ophthalmic composition comprising an oxidatively unstable excipient wherein said container comprises the polymerization product of an effective amount of a stabilizing agent functionalized with a polymerizable group and at least one type of polymerizable momoner. 66. The container of claim 65 wherein the stabilizing agent is selected from the group consisting of silica, chitin derivative such as chitosan, cellulose its derivatives, and N,N,N',N',N", N"-hexa(2-pyridyl)-1,3,5- tris(aminomethyl)benzene, diethylenetriaminepentaacetic acid, and salts of diethylenetriaminepentaacetic acid, and acid. 67. The container of claim 65 wherein the stabilizing agent is selected from the group consisting of diethylenetriaminepentaacetic acid, and salts of diethylenetriaminepentaacetic acid. Ophthalmic compositions and methods of preparing such compositions are disclosed.

Full Text

METHODS FOR STABILIZING OXIDATIVELY UNSTABLE COMPOSITIONS
FIELD OF THE INVENTION
This invention related to compositions of oxidatively unstable ophthalmic
ingredients and methods for preparing such compositions
RELATED APPLICATION
This application claims priority from a non-provisional filing, U.S. App.
Pat. No. U.S. App. Pat. No. 60/783,557 entitled "Methods for Stabilizing
Oxidatively Unstable Pharmaceutical Compositions," which was filed on, March
17, 2006.
BACKGROUND
Therapeutic agents for topical administration to the eye are generally
formulated in either a liquid or gel form and must be kept sterile until
administration. Accordingly, ophthalmic therapeutic agents are either
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packaged asceptically, which is cumbersome and expensive or are heat
sterilized. Unfortunately, many therapeutic agents are not oxidatively stable,
especially at elevated temperatures.
EDTA, Dequest, and Desferal have been used to improve the stability of
certain therapeutic agents during autoclaving. However, there remains a need
for other compounds capable of stabilizing unstable therapeutic agents that are
susceptible to oxidative degradation. This need is met by the following
invention.
BRIEF DESCRIPTION OF THE DRAWINGS
Fig. 1 Stability Study with Ketotifen and PAA or EDTA
Fig. 2. Stability Study with Ketotifen and PAA or EDTA
Fig. 3 Stability Study with Ketotifen and PAA or DTPA
DETAILED DESCRIPTION OF THE INVENTION
This invention includes a method of stabilizing an ophthalmic
composition comprising an oxidatively unstable pharmaceutical ingredient
wherein said method comprises adding an effective amount of a stabilizing
agent to the ophthalmic composition.
As used herein "oxidatively unstable pharmaceutical ingredient" refers to
pharmaceutical or nutraceutical compounds used to treat conditions of the eye,

and such compound degrade in the presence of oxygen and certain transition
metals. Examples of pharmaceutical compounds include antihistamines,
antibiotics, antibacterial agents, antiviral agents, antifungal agents, analgesics,
anesthetics, antiallergeneic agents, mast cell stabilizers, steroidal and non-
steroidal anti-inflammatory agents, angiogenesis inhibitors; antimetabolites,
fibrinolytics, neuroprotective drugs, angiostatic steroids, mydriatics.cyclopegic
mydriatics; miotics; vasoconstrictors; vasodilators, anticlotting agents;
anticancer agents, antisense agents, immunomodulatory agents, carbonic
anhydrase inhibitors, integrin antabonistsl; cyclooxygenase inhibitors, VEGF
antagonists; immunosuppressant agents and the like. Particularly, examples of
pharmaceutical agents include but are not limited to acrivastine, antazoline,
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astemizole, azatadine, azelastine, buclizine, bupivacaine, cetirizine, clemastine,
cyclizine, cyproheptadine, ebastine, emedastine, ephedrine, eucatropine,
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fexofenadine, homatropine, hydroxyzine, ketotifen, levocabastine,
levoceterizine, lomefloxacin, meclizine, mepivacaine, mequitazine,
methdilazine, methapyrilene, mianserin, naphazoline norastemizole,
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norebastine, ofloxacin, oxymetazoline, pheniramine, phenylephrine,
physostigmine, picumast, promethazine, scopolamine, terfenadine,
tetrahydozoline, thiethylperazine, timolol, trimeprazine, triprolidine,
pharmaceutically acceptable salts and mixtures thereof. Preferred
pharmaceutical compounds include acrivatine, antazoline, astemizole,
azatadine, azelastine, clemastine, cyproheptadine, ebastine, emedastine,
eucatropine, fexofenadine, homatropine, hydroxyzine, ketotife, levocabastine,
levoceterizine, meclizine, mequitazine, methdialazine, methapyrilene,
norastemizole, norebastine, oxymetazoline, physootigmine, picumast,
promethazine, scopolamine, terfenadine, tetrahyerozoline, fimilol, trimeprazine,
triprolidine, and pharmaceutically acceptable salts thereof. Particularly
preferred pharmaceutical compounds include phenarimine, ketotifen, ketotifen
fumarate nor ketotifen.olapatadine and mixtures thereof. More particularly
preferred pharmaceutical compounds include ketotifen, its pharmaceutically
acceptable salts, and mixtures thereof.
Examples of nutraceutical compounds include vitamins and supplements
such as vitamins A, D, E, lutein, zeaxanthin, lipoic acid, flavonoids,

ophthalmicially compatible fatty acids, such as omega 3 and omega 6 fatty
acids, combinations thereof, combinations with pharmaceutical compounds and
the like. Preferred pharmaceutical or nutraceutical compounds are those that
degrade when solutions of these compounds and oxidative catalysts (such as
metals and metallic salts) are mixed together at ambient or elevated
temperatures, as compared to solutions of these compounds without oxidative
catalysts at ambient or elevated temperatures. Particularly preferred
pharmaceutical or nutraceutical compounds are those that degrade greater
than about 10% when heated to about 120°C for about 20 minutes with
oxidative catalysts. The concentration of oxidatively unstable pharmaceutical
ingredients in the ophthalmic compositions of the invention range from about 2
μg/mL to about 6.5 g/mL, particularly preferred, about 0.1 μg/mL to about
10,000 μg/mL.
The term "ophthalmic composition" refers to liquids, aerosols, or gels
that may be topically administered to the eye. The term "stabilizing agent"
refers to chelant compositions that inhibit metal catalyzed oxidative degradation
of the oxidatively unstable pharmaceutical ingredient. Examples of stabilizing
agents include but are not limited to silica, chitin derivative such as chitosan,
polyamides such as poly(aspartic acid-co-w-amino acid (See CAN:129:54671,
Shibata, Minako et al. Graduate School Environmental Earth Science,
Hokkaido University, Sapporo, Japan Macromolecular Symposia (1998),
130, 229-244) and polymeric amides such as poly[iminocarbonyl(2,5-dihydroxy-
1,4-phenylene)carbonylimino-1,4-phenylenemethylene-1,4-phenylene], CAS #
87912-00-3, polymeric lactams such as polyvinylpyrrolidone, polyamino
carboxylic acids such as diethylenetriaminepentaacetic acid and
triethylenetriaminepentaacetic acid, polymeric amines such as polyallylamine,
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crown ethers such as 18-crown-6, 21-crown-7, and 24-crown-8, cellulose and
its derivatives, and N,N,N',N',N", N"-hexa(2-pyridyl)-1,3,5-
tris(aminomethyl)benzene, and certain macrocyclic ligands such as crown
ethers, ligand containing knots and catenands (See, David A. Leigh et al
Angew. Chem Int. Ed., 2001, 40, No. 8, pgs. 1538-1542 and Jean-Claude
Chambron et al. Pure & Appl. Chem., 1990, Vol. 62, No. 6, pgs. 1027-1034)
The preferred stabilizing agents are polyamino carboxylic acids such as

diethylenetriaminepentaacetic acid and triethylenetriaminepentaacetic acidi
The particulary preferred stabilizing agents are diethylenetriaminepentaacetic
acid ("DTPA"), or salts of DTPA such as CaNa3DTPA, ZnNa3DTPA, and
Ca2DTPA. The term "effective amount" refers to the amount of stabilizing agent
required to inhibit the oxidative degradation of the pharmaceutical ingredient.
In most circumstances it is preferred that there is a 1:1 molar ration of metal
present in the ophthalmic composition to chelant, is more preferably about 1 of
metal to greater than about 1 of chelant compositions, most preferably about 1
of metal to greater than or equal to about 2 of chelant compositions. With
respect to concentration limits, it is preferred that the stabilizing agents have a
concentration in the ophthalmic composition from about 2.5 μmoles/liter to
about, 5000 μmoles/liter more preferably from about 20 μmoles/liter to about
1000 μmoles/liter, more preferably from about 100 μmoles/liter to about
1000 μmoles/liter, most preferably from about 100 μmoles/liter to about
500 μmoles/liter.
Aside from the oxidatively unstable pharmaceutical ingredient and the
stabilizing agent, the ophthalmic composition contains suitable ophthalmic
carriers. Suitable carriers include antioxidants (radical scavengers),
demulcents, antibacterial agents, solubilizers, surfactants, buffer agents,
tonicity adjusting agents, chelating agents, preservatives, wetting agents,
thickeners, water, saline solution, mineral oil, petroleum jelly, water soluble
solvents, such as C15-20 alcohols, C15-20 amides, C15-20 alcohols substituted with
zwitterions, vegetable oils or mineral oils comprising from 0.5 to 5% by weight
hydroxyethylcellulose, ethyl oleate, carboxymethylcellulose, polyvinyl-
pyrrolidone and other non-toxic water-soluble polymers for ophthalmic uses,
such as, for example cellulose derivatives, such as methylcellulose, alkali metal
salts of carboxy-methylcellulose, hydroxymethylcellulose,
hydroxyethylcellulose, methylhydroxypropyl-cellulose, hydroxypropylcellulose,
chitosan and scleroglucan, acrylates or methacrylates, such as salts of
poly(acrylic acid) or ethyl acrylate, polyacrylamides, natural products, such as
gelatin, alginates, pectins, tragacanth, karaya gum, xanthan gum, carrageenin,
agar and acacia, starch derivatives, such as starch acetate and hydroxypropyl
starch, and also other synthetic products, such as poloxamers, e.g. Poloxamer

F127, polyvinyl alcohol, polyvinylpyrrolidone, polyvinyl methyl ether,
polyethylene oxide, preferably cross-linked poly(acrylic acid), such as neutral
Carbopol, or mixtures of those polymers. Preferred carriers are water,
cellulose derivatives, such as methylcellulose, alkali metal salts of
carboxymethylcellulose, hydroxymethylcellulose, hydroxyethylcellulose,
methylhydroxypropylcellulose and hydroxypropylcellulose, neutral Carbopol, or
mixtures thereof. The concentration of the carrier is, for example, from 0.1 to
100000 times the concentration of the active ingredient combinations thereof
and the like. When the ophthalmic composition is an eye drop, preferred
carriers include water, pH buffered saline solution, mixtures thereof and the
like. The preferred carrier is an aqueous saline solution containing salts
including, without limitation, sodium chloride, sodium borate, sodium
phosphate, sodium hydrogenphosphate, sodium dihydrogenphosphate, or the
corresponding potassium salts of the same. These ingredients are generally
combined to form buffered solutions that include an acid and its conjugate
base, so that addition of acids and bases cause only a relatively small change
in pH. The buffered solutions may additionally include 2-(N-
morpholino)ethanesulfonic acid (MES), sodium hydroxide, 2,2-
bis(hydroxymethyl)-2,2',2"-nitrilotriethanol, n-tris(hydroxymethyl)methyl-2-
aminoethanesulfonic acid, citric acid, sodium citrate, sodium carbonate, sodium
bicarbonate, acetic acid, sodium acetate, ethylenediamine tetraacetic acid and
the like and combinations thereof. Most preferably, the carrier is a borate
buffered or phosphate buffered saline solution.
Further the invention includes an ophthalmic composition comprising an
oxidatively unstable pharmaceutical ingredient and an effective amount of a
stabilizing agent. The terms oxidatively unstable pharmaceutical ingredient,
effective amount, and stabilizing agents all have their aforementioned
meanings and preferred ranges.
Still further the invention includes a method of stabilizing an ophthalmic
composition comprising an oxidatively unstable excipient wherein said method
comprises adding an effective amount of a stabilizing agent to the ophthalmic
i
composition.

As used herein "oxidatively unstable excipient" refers to a component of
ophthalmic compositions that degrades in the presence of oxygen and certain
transition metals. Examples of unstable excipients include but are not limited to
astringents, demulcents, emollients, hypertonicity agents, oleaginous, agents,
tonicity agents mucomimetic agents, and the like. Particularly examples of
unstable excipients include but are not limited to cellulose derivatives,
carboxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose,
hyaluronic acid, methylcellulose, Dextran, gelatin, polyols, glycerin,
polyethylene glycol, polysorbate, propylene glyxol, polyvinyl alcohol, povidone
lanolin, mineral oil, paraffin, petrolatum, white ointment, white petrolatum,
white, wax, and yellow wax. The terms "stabilizing agent," and "effective
amount" have their aforementioned meanings and preferred ranges.
i
Yet still further the invention includes an ophthalmic composition
i
comprising an oxidatively unstable excipient and an effective amount of a
stabilizing agent. The terms "oxidatively unstable excipient," "stabilizing agent,"
and "effective amount" have their aforementioned meanings and preferred
ranges.
Even further still the invention includes a method of stabilizing an
ophthalmic composition comprising an oxidatively unstable pharmaceutical
ingredient wherein said method comprises
(a) functionalizing said stabilizing agent with a polymerizable group,
(b) polymerizing an effective amount of the product of step (a) with at least
one type of polymerizable monomer, and
(c) contacting the ophthalmic composition comprising an oxidatively
unstable pharmaceutical ingredient with the polymer of step (b).
The terms oxidatively unstable pharmaceutical ingredient, effective amount,
and stabilizing agents all have their aforementioned meanings and preferred
ranges. The term functionalizing means chemically bonding a polymerizable
group to said stabilizing agent. Examples of a polymerizable group includes
but are not limited to methacrylate, acrylate, acrylamide, and styrene. The term
polymerizable monomer includes compounds containing olefinic moieties
capable of adding to radical species, such as propylene, ethylene and the like.
The polymerization product of step (b) may be soluble or insoluble in the

ophthalmic composition. It is preferred that the polymerization product of step
(b) is not soluble in the ophthalmic composition and as such the polymerization
product of step (b) may be in any form such as rods, discs, containers, films
and the like.
Even further still the invention includes a method of stabilizing an
ophthalmic composition comprising an oxidatively unstable excipient wherein
said method comprises
(a) functionalizing said stabilizing agent with a polymerizable group,
(b) polymerizing an effective amount of the product of step (a) with at least
one type of polymerizable monomer, and
(c) contacting the ophthalmic composition comprising an oxidatively
unstable exicipient with the polymer of step (b).
i
The terms oxidatively unstable excipient, effective amount, stabilizing agents,
polymerizable group, and polymerizable monomers all have their
aforementioned meanings and preferred ranges.
Further still the invention includes a container for an ophthalmic
composition comprising an oxidatively unstable pharmaceutical ingredient
wherein said container comprises the polymerization product of an effective
amount of a stabilizing agent functionalized with a polymerizable group and at
least one type of polymerizable momoner. The terms oxidatively unstable
pharmaceutical ingredient, effective amount, stabilizing agents, polymerizable
group, and polymerizable monomers all have their aforementioned meanings
and preferred ranges.
Yet, further still the invention includes a container for an ophthalmic
composition comprising an oxidatively unstable excipient wherein said
container comprises the polymerization product of an effective amount of a
stabilizing agent functionalized with a polymerizable group and at least one
type of polymerizable momoner. The terms oxidatively unstable excipient,
effective amount, stabilizing agents, polymerizable group, and polymerizable
monomers all have their aforementioned meanings and preferred ranges.
The advantages of the compositions and methods of this invention are
numerous. First ketotifen is known as a oxidatively unstable pharmaceutical
i
ingredient. Compositions containing ketotifen fumarate are known. These

compositions contain EDTA and the pH of those compositions is about 5.5.
These EDTA solutions stabilize the ketotifen fumarate against oxidative degradation, the pH of these solutions is below the threshold for ocular
awareness and it is likely that some patient who use this solution will be
uncomfortable due to low pH value of the solution. See Tang, I., Wong, D.M.,
Yee, D.J. and Harris, M.G. 1996 The pH of multi-purpose soft contact lens
solutions. Optom. Vis. Sci. 73:746-749. Adler, F.H. 1959 Physiology of the
Eye. Third edition, p.40. Brawner, L.W. and Jessop, D.G. 1962. A review of
contact lens solutions. Contacto 6:49-51. It has been discovered that the
stabilizing agents of the invention will reduce the degradation of ketotifen
fumarate at higher pH values that those of ketotifen fumarate solutions
containing EDTA. The pH of ophthalmic compositions of the invention is
preferably between about pH 6.6 and about pH 7.2, more preferably between
about pH 6.8 and about pH 7.0.
Second it has been shown that the shelf life of ketotifen fumarate
i
solutions containing DTPA are superior to the shelf life of ketotifen fumarate
solutions containing either EDTA or PAA. Third, it is known that the application
of heat increases the rate of degradation of many pharmaceutical ingredients.
i
It has been shown that the stabilizing agents of this invention are useful in
reducing degradation associated with higher temperature, such as sterilization
temperatures.
In order to illustrate the invention the following examples are included.
These examples do not limit the invention. They are meant only to suggest a
i
method of practicing the invention. Those knowledgeable in contact lenses as
well as other specialties may find other methods of practicing the invention.
However, those methods are deemed to be within the scope of this invention.
EXAMPLES
The following abbreviations are used below
PAA
Polyacrylic Acid, sodium salt having an average molecular weight
of 200,000
EDTA
Ethylenediamine tetraacetic acid.

Solution A
Deionized water containing the following ingredients by weight: NaCI
(0.83%), Boric Acid (0.91%), Sodium tetraborate decahydrate (0.1%)
Initial Testing to Determine Suitability of Ingredients
It is known that excessive quantities of certain transition metals and their
salts will degrade ketotifen fumarate. The amount of metals and salts
contained within commercially available ingredients varies, so test
batches of Solution A were evaluated as follows. 200 g Solution A was
mixed at ambient temperature and ketotifen fumarate (5 mg + 2 mg) was
added and mixed until homogenous. Six glass vials were filled with 3ml_
of this solution. The vials were stopped with poly tetrafluoroethylene
("PTFE ") and three of the vials were heated for eighteen minutes at
124 °C. Samples of each treated vial (1.0-1.5 mL) were analyzed by
HPLC and compared to the untreated controls. If the amount of
ketotifen in the treated vials reduced by less than or equal to five percent
(≤5%) the ingredients were determined to be suitable for further studies
and larger batches of Solution A were prepared from these ingredients.
Example 1
Preparation of Ketotifen Fumarate Solutions with PAA. EDTA
PAA (2000 μg/mL) was added to Solution A, and 50 μg/ml_ of ketotifen
fumarate (approximately 36 μg/mL of ketotifen) was dissolved in the system.
EDTA (100 μg/mL) was added to another batch of Solution A, and 50 μg/mL of
ketotifen fumarate was dissolved in the system. Negative One and negative
twelve (-1.0 and 12.0) diopter etafilcon A contact lenses were added to vials
containing 3mL of each of the above solutions. The vials were sealed with
PTFE coated rubber stoppers, sterilized at 124 °C for 18 minutes, and stored at
ambient temperature for one year. Samples were harvested throughout the
year and analyzed for the presence of ketotifen by HPLC. The results are
presented in Figures 1 and 2. These results show that there is substantial
degradation of the ketotifen over time with PAA and with EDTA.

Example 2
Preparation of Ketotifen Fumarate Solutions with PAA. DTPA ;
To separate batches of Solution A, Ca2DTPA (100 μg/mL and 300
μg/mL, 213 and 640 μmoles/L respectively) and 2000 μg/mL (0.2%,
approximately 18.51 mmole/L methacrylate content) of PAA were added, and
approximately 25 μg/mL of ketotifen fumarate (approximately 18 μg/mL of
ketotifen) was dissolved in each of the systems. Three mL samples of each
solution were added to individual vials containing contact lenses. Each set of
vials was closed with PTFE stoppers, sterilized at 124 °C for 18 minutes, and
stored at 80°C for a period of two weeks. Samples were harvested at various
intervals and analyzed for the presence of ketotifen by HPLC. The results are
presented in Figure 3. These results show that there is substantial degradation
of the ketotifen over time with PAA but not with the calcium salt of DTPA
Example 3
Ketotifen Fumarate Solutions with oxidation catalysts
Ketotifen fumarate (50μg/ml_, approximately 36μg/mL of ketotifen) was
dissolved in Solution A containing approximately 500 μg/mL of either DTPA
(approximately 1272 μmoles/L of DTPA) or the sodium salt of EDTA
(Na2C10H1408N2•2 H2O, 1344 μmoles/L of EDTA). Approximately 50 μg/mL of
the salts listed in Table 1 were dissolved in each of the solutions and about
3ml_ of each solution was dosed into several vials The vials were sealed With
PTFE coated rubber stoppers and were subjected to zero, one, two or three
sterilization cycles as indicated in Table 1. One sterilization cycle is eighteen
minutes of heating at 124 °C. Samples were analyzed by HPLC for the
concentration of ketotifen at the intervals indicated in Table 2. This data
shows that in the presence of oxidative catalysts, DTPA reduces the amount of
oxidative degradation as compared to EDTA.

What is claimed is
1. A method of stabilizing an ophthalmic composition comprising an
oxidatively unstable pharmaceutical ingredient wherein said method comprises
adding an effective amount of a stabilizing agent to the ophthalmic composition.
2. The method of claim 1 wherein the stabilizing agent is selected from the
group consisting of silica, chitin derivative such as chitosan, cellulose its
derivatives, and N,N,N',N',N", N"-hexa(2-pyridyl)-1,3,5-
tris(aminomethyl)benzene, diethylenetriaminepentaacetic acid, and salts of
diethylenetriaminepentaacetic.
3. The method of claim 1 wherein the stabilizing agent is selected from the
group conisiting of diethylenetriaminepentaacetic acid, and salts of
diethylenetriaminepentaacetic acid.
4. The method of claim 1 wherein the effective amount of the stabilizing
agent is about 2.5 μmoles/liter to about 5000 μmoles/liter.
5. The method of claim 1 wherein the effective amount of the stabilizing
agent is about 20 μmoles/liter to about 1000 μmoles/liter.
6. The method of claim 1 wherein the effective amount of the stabilizing
agent is about 100 μmoles/liter to about 600 μmoles/liter.
7. The method of claim 1 wherein the stabilizing agent is
diethylenetriaminepentaacetic acid.
8. The method of claim 1 wherein the stabilizing agent is
diethylenetriaminepentaacetic acid or salts there of and the effective amount of
said stabilizing agent is about 100 μmoles/liter to about 1000 μmoles/liter
9. The method of claim 1 wherein the oxidatively unstable pharmaceutical
ingredient is selected from the group consisting of acrivastine, antazoline,

astemizole, azatadine, azelastine, buclizine, bupivacaine, cetirizine, clemastine,
cyclizine, cyproheptadine, ebastine, emedastine, eucatropine, fexofenadine,
homatropine, hydroxyzine, ketotifen, levocabastine, levoceterizine,
lomefloxacin, meclizine, mepivacaine, mequitazine, methdilazine,
methapyrilene, mianserin, norastemizole, norebastine, ofloxacin,
oxymetazoline, pheniramine, physostigmine, picumast, promethazine,
scopolamine, terfenadine, tetrahydozoline, thiethylperazine, timolol,
trimeprazine, triprolidine, pharmaceutically acceptable salts and mixtures
thereof.
10. The method of claim 1 wherein the oxidatively unstable pharmaceutical
ingredient is selected from the group consisting of acrivatine, antazoline,
astemizole, azatadine, azelastine, clemastine, cyproheptadine, ebastine,
emedastine, eucatropine, fexofenadine, homatropine, hydroxyzine, ketotifen,
levocabastine, levoceterizine, meclizine, mequitazine, methdialazine,
methapyrilene, norastemizole, norebastine, oxymetazoline, physootigmine,
picumast, promethazine, scopolamine, terfenadine, tetrahyerozoline, fimilol,
trimeprazine, triprolidine, and pharmaceutically acceptable salts thereof.
11. The method of claim 1 wherein the oxidatively unstable pharmaceutical
ingredient is selected from the group consisting of phenarimine, ketotifen,
i
ketotifen fumarate, nor ketotifen fumarate olopatadine and mixtures thereof.
12. The method of claim 1 wherein the oxidatively unstable pharmaceutical
ingredient is selected from the group consisting of ketotifen, its
pharmaceutically acceptable salts, and mixtures thereof.
i
13. The method of claim 1 wherein the oxidatively unstable pharmaceutical
ingredient is selected from the group consisting of vitamins A, D, E, lutein,
zeaxanthin, lipoic acid, flavonoids, ophthalmicially compatible fatty acids, such
as omega 3 and omega 6 fatty acids and combinations thereof.

14. The method of claim 1 wherein the stabilizing agent is
diethylenetriaminepentaacetic acid or salts there of, the effective amount of
said stabilizing agent is about 100 μmoles/liter to about 1000 μmoles/liter, and
the oxidatively unstable pharmaceutical ingredient is selected from the group
consisting of ketotifen, its pharmaceutically acceptable salts, and mixtures
thereof.
15. The method of claim 1 wherein the stabilizing agent is the calcium salt
diethylenetriaminepentaacetic acid, the effective amount of said stabilizing
agent is about 100 μmoles/liter to about 1000 μmoles/liter, and the oxidatively
unstable pharmaceutical ingredient is selected from the group consisting of
ketotifen, pharmaceutically acceptable salts of ketotifen and mixtures thereof.
16. The method of claim 1 wherein the ophthalmic composition has a pH of
about 6.6 to about 7.2.
17. The method of claim 1 wherein the ophthalmic composition has a pH of
about 6.8 to about 7.2.
18. An ophthalmic composition comprising an oxidatively unstable
pharmaceutical ingredient and an effective amount of a stabilizing agent.
19. The ophthalmic composition of claim 18 wherein the stabilizing agent is
selected from the group consisting of silica, chitin derivative such as chitosan,
cellulose its derivatives, and N,N,N',N',N", N"-hexa(2-pyridyl)-1,3,5-
tris(aminomethyl)benzene, diethylenetriaminepentaacetic acid, and salts of
diethylenetriaminepentaacetic acid, and acid.
20. The ophthalmic composition of claim 18 wherein the stabilizing agent is
selected from the group conisiting of diethylenetriaminepentaacetic acid, and
salts of diethylenetriaminepentaacetic acid.

21. The ophthalmic composition of claim 18 wherein the effective amount of
the stabilizing agent is about 2.5 μmoles/liter to about 5000 μmoles/liter.
22. The ophthalmic composition of claim 18 wherein the effective amount of
the stabilizing agent is about 100 μmoles/liter to about 1000 μmoles/liter.
23. The ophthalmic composition of claim 18 wherein the effective amount of
the stabilizing agent is about 100 μmoles/liter to about 600 urnoles/liter.
24. The ophthalmic composition of claim 18 wherein the stabilizing agent is
diethylenetriaminepentaacetic acid
25. The ophthalmic composition of claim 18 wherein the stabilizing agent is
diethylenetriaminepentaacetic acid or salts there of and the effective amount of
said stabilizing agent is about 100 μmoles/liter to about 1000 μmoles/liter
26. The ophthalmic composition of claim 18 wherein the oxidatively unstable
pharmaceutical ingredient is selected from the group consisting of acrivastine,
antazoline, astemizole, azatadine, azelastine, buclizine, bupivacaine, cetirizine,
clemastine, cyclizine, cyproheptadine, ebastine, emedastine, eucatropine,
fexofenadine, homatropine, hydroxyzine, ketotifen, levocabastine,
levoceterizine, lomefloxacin, meclizine, mepivacaine, mequitazine,
methdilazine, methapyrilene, mianserin, norastemizole, norebastine, ofloxacin,
oxymetazoline, pheniramine, physostigmine, picumast, promethazine,
scopolamine, terfenadine, tetrahydozoline, thiethylperazine, timolol,
trimeprazine, triprolidine, pharmaceutically acceptable salts and mixtures
thereof.
27. The ophthalmic composition of claim 18 wherein the oxidatively unstable

pharmaceutical ingredient is selected from the group consisting of acrivatine,
antazoline, astemizole, azatadine, azelastine, clemastine, cyproheptadine,
ebastine, emedastine, eucatropine, fexofenadine, homatropine, hydroxyzine,
ketotifen, levocabastine, levoceterizine, meclizine, mequitazine, methdialazine,

methapyrilene, norastemizole, norebastine, oxymetazoline, physootigmine,
picumast, promethazine, scopolamine, terfenadine, tetrahyerozoline, fimiloj,
trimeprazine, triprolidine, and pharmaceutically acceptable salts thereof.
28. The ophthalmic composition of claim 18 wherein the oxidatively unstable
pharmaceutical ingredient is selected from the group consisting of phenarimine,
ketotifen, ketotifen fumarate nor ketotifen fumarate, olopatadine and mixtures
thereof.
29. The ophthalmic composition of claim 18 wherein the oxidatively unstable
pharmaceutical ingredient is selected from the group consisting of ketotifen, its
pharmaceutically acceptable salts, and mixtures thereof.
30. The ophthalmic composition of claim 18 wherein the oxidatively unstable
pharmaceutical ingredient is selected from the group consisting of vitamins A,
D, E, lutein, zeaxanthin, lipoic acid, flavonoids, ophthalmicially compatible fatty
acids, such as omega 3 and omega 6 fatty acids and combinations thereof.
31. The ophthalmic composition of claim 18 wherein the stabilizing agent is
diethylenetriaminepentaacetic acid or salts there of, the effective amount of
said stabilizing agent is about 200 μmoles/liter to about 1000 μmoles/liter, and
the oxidatively unstable pharmaceutical ingredient is selected from the group
consisting of ketotifen, its pharmaceutically acceptable salts, and mixtures
thereof.
32. The ophthalmic composition of claim 18 wherein the stabilizing agent is
the calcium salt diethylenetriaminepentaacetic acid, the effective amount of
said stabilizing agent is about 100 μmoles/liter to about 1000 μmoles/liter, and
the oxidatively unstable pharmaceutical ingredient is selected from the group
consisting of ketotifen, pharmaceutically acceptable salts of ketotifen and
mixtures thereof.

33. The ophthalmic composition of claim 18 wherein the pH is about 6.6 to
about 7.2.
34. The ophthalmic composition wherein the pH is about 6.8 to about 72.
35. A method of stabilizing an ophthalmic composition comprising an
oxidatively unstable excipient wherein said method comprises adding an
effective amount of a stabilizing agent to the ophthalmic composition.
36. The method of claim 35 wherein the oxidatively unstable excipient is
selected from the group consisting of cellulose derivatives,
carboxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose,
hyaluronic acid, methylcellulose, Dextran, gelatin, polyols, glycerin,
polyethylene glycol, polysorbate, propylene glyxol, polyvinyl alcohol, povidone
lanolin, mineral oil, paraffin, petrolatum, white ointment, white petrolatum,
white, wax, and yellow wax.
37. The method of claim 35 wherein the stabilizing agent is selected from
the group consisting of silica, chitin derivative such as chitosan, cellulose its
derivatives, and N,N,N\N',N", N"-hexa(2-pyridyl)-1,3,5-
tris(aminomethyl)benzene, diethylenetriaminepentaacetic acid, and salts of
diethylenetriaminepentaacetic acid.
38. The method of claim 35 wherein the stabilizing agent is selected from
the group conisiting of diethylenetriaminepentaacetic acid, and salts of
diethylenetriaminepentaacetic acid.
39. The method of claim 35 wherein the effective amount of the stabilizing
agent is about 20 μmoles/liter to about 1000 μmoles/liter.
40. The method of claim 35 wherein the effective amount of the stabilizing
agent is about 100 μmoles/liter to about 600 μmoles/liter.

41. The method of claim 35 wherein the stabilizing agent is
diethylenetriaminepentaacetic acid.
42. The method of claim 35 wherein the stabilizing agent is
diethylenetriaminepentaacetic acid or salts there of and the effective amount of
said stabilizing agent is about 100 μmoles/liter to about 1000 |jmoles/liter !
43. The method of claim 35 wherein the stabilizing agent is the calcium salt
diethylenetriaminepentaacetic acid, the effective amount of said stabilizing
agent is about 100 μmoles/liter to about 1000 μmoles/liter, and the oxidatively
unstable excipient is selected from the group consisting of
carboxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose,
hyaluronic acid, methylcellulose, Dextran, gelatin, polyols, glycerin,
polyethylene glycol, polysorbate, propylene glyxol, polyvinyl alcohol, and
mixtures thereof.
44. The method of claim 35 wherein the ophthalmic composition has a pH of
about 6.6 to about 7.2.
45. The method of claim 35 wherein the ophthalmic composition has a pH of

about 6.8 to about 7.2.
46. An ophthalmic composition comprising an oxidatively unstable excipient
and an effective amount of a stabilizing agent.
47. The ophthalmic composition of claim 46 wherein the stabilizing agent is
selected from the group consisting of silica, chitin derivative such as chitosan,
cellulose its derivatives, and N.N.N'.N'.N", N"-hexa(2-pyridyl)-1,3,5-
tris(aminomethyl)benzene, diethylenetriaminepentaacetic acid, and salts of
diethylenetriaminepentaacetic acid, and acid.

48. The ophthalmic composition of claim 46 wherein the stabilizing agent is
selected from the group conisiting of diethylenetriaminepentaacetic acid, and
salts of diethylenetriaminepentaacetic acid.
49. The ophthalmic composition of claim 46 wherein the effective amount of
the stabilizing agent is about 20 μmoles/liter to about 1000 μmoles/liter.
50. The ophthalmic composition of claim 46 wherein the effective amount of
the stabilizing agent is about 100 μmoles/liter to about 600 μmoles/liter.
51. The ophthalmic composition of claim 46 wherein the stabilizing agent is
i
diethylenetriaminepentaacetic acid.
52. The ophthalmic composition of claim 46 wherein the stabilizing agent is
diethylenetriaminepentaacetic acid or salts there of and the effective amount of
said stabilizing agent is about 100 μmoles/liter to about 1000 μmoles/liter
53. The ophthalmic composition of claim 46 wherein the stabilizing agent is
the calcium salt diethylenetriaminepentaacetic acid, the effective amount of
said stabilizing agent is about 100 μmoles/liter to about 1000 μmoles/liter, and
the oxidatively oxidatively unstable excipient is selected from the group
consisting of carboxymethylcellulose, hydroxyethylcellulose,
hydroxypropylcellulose, hyaluronic acid, methylcellulose, Dextran, gelatin,
polyols, glycerin, polyethylene glycol, polysorbate, propylene glyxol, polyvinyl
alcohol, and mixtures thereof.
54. The ophthalmic composition of claim 46 wherein the pH is about 6.6 to
about 7.2.
55. The ophthalmic composition of claim 46 wherein the pH is about 6.8 to
about 7.2.

56. A method of stabilizing an ophthalmic composition comprising an
oxidatively unstable pharmaceutical ingredient wherein said method comprises
(a) functionalizing said stabilizing agent with a polymerizable group,
(b) polymerizing an effective amount of the product of step (a) with at least
one type of polymerizable monomer, and
(c) contacting the ophthalmic composition comprising an oxidatively
unstable pharmaceutical ingredient with the polymer of step (b).

57. The method of claim 56 wherein the stabilizing agent is selected from
the group consisting of silica, chitin derivative such as chitosan, cellulose its
derivatives, and N.N.N'.N'.N", N"-hexa(2-pyridyl)-1,3,5-
tris(aminomethyl)benzene, diethylenetriaminepentaacetic acid, and salts of
diethylenetriaminepentaacetic acid, and acid.
58. The method of claim 56 wherein the stabilizing agent is selected from
the group consisting of diethylenetriaminepentaacetic acid, and salts of
diethylenetriaminepentaacetic acid.
59. A method of stabilizing an ophthalmic composition comprising an
oxidatively unstable excipient wherein said method comprises

(a) functionalizing said stabilizing agent with a polymerizable group,
(b) polymerizing an effective amount of the product of step (a) with at least
one type of polymerizable monomer, and
(c) contacting the ophthalmic composition comprising an oxidatively
unstable exicipient with the polymer of step (b).
60. The method of claim 59 wherein the stabilizing agent is selected from
the group consisting of silica, chitin derivative such as chitosan, cellulose its
derivatives, and N,N,N',N',N", N"-hexa(2-pyridyl)-1,3,5-
tris(aminomethyl)benzene, diethylenetriaminepentaacetic acid, and salts of
diethylenetriaminepentaacetic acid, and acid.

61. The method of claim 59 wherein the stabilizing agent is selected from
the group consisting of diethylenetriaminepentaacetic acid, and salts of
diethylenetriaminepentaacetic acid.
62. A container for an ophthalmic composition comprising an oxidatively
unstable excipient wherein said container comprises the polymerization product
of an effective amount of a stabilizing agent functionalized with a polymerizable
group and at least one type of polymerizable momoner.
63. The container of claim 62 wherein the stabilizing agent is selected from
the group consisting of silica, chitin derivative such as chitosan, cellulose its
derivatives, and N,N,N',N',N", N"-hexa(2-pyridyl)-1,3,5-
tris(aminomethyl)benzene, diethylenetriaminepentaacetic acid, and salts of
diethylenetriaminepentaacetic acid, and acid.
64. The container of claim 62 wherein the stabilizing agent is selected from
the group consisting of diethylenetriaminepentaacetic acid, and salts of
diethylenetriaminepentaacetic acid.
65. A container for an ophthalmic composition comprising an oxidatively
unstable excipient wherein said container comprises the polymerization product
of an effective amount of a stabilizing agent functionalized with a polymerizable
group and at least one type of polymerizable momoner.
66. The container of claim 65 wherein the stabilizing agent is selected from
the group consisting of silica, chitin derivative such as chitosan, cellulose its
derivatives, and N,N,N',N',N", N"-hexa(2-pyridyl)-1,3,5-
tris(aminomethyl)benzene, diethylenetriaminepentaacetic acid, and salts of
diethylenetriaminepentaacetic acid, and acid.
67. The container of claim 65 wherein the stabilizing agent is selected from
the group consisting of diethylenetriaminepentaacetic acid, and salts of
diethylenetriaminepentaacetic acid.

Ophthalmic compositions and methods of preparing such compositions are disclosed.

Documents:

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Patent Number

269171

Indian Patent Application Number

3769/KOLNP/2008

PG Journal Number

41/2015

Publication Date

09-Oct-2015

Grant Date

07-Oct-2015

Date of Filing

15-Sep-2008

Name of Patentee

JOHNSON & JOHNSON VISION CARE, INC.

Applicant Address

7500 CENTURION PARKWAY, SUITE 100 JACKSONVILLE, FLORIDA

Inventors:

#	Inventor's Name	Inventor's Address
1	FRANK MOLOCK	1543 WILDFERN DRIVE, ORANGE PARK, FL 32003
2	SHIVKUMAR MAHADEVAN	1905 WHITE DOGWOOD LANE, ORANGE PARK, FL 32003

PCT International Classification Number

A61K 9/00,A61K 47/18

PCT International Application Number

PCT/US2007/064142

PCT International Filing date

2007-03-16

PCT Conventions:

#	PCT Application Number	Date of Convention	Priority Country
1	60/783,557	2006-03-17	U.S.A.